文献检索是系统评价(SR)的基石,但其报告质量长期存在不足,严重影响研究的透明度与可重复性。PRISMA-S(PRISMA for Searching)作为PRISMA 2020的扩展版本,为文献检索报告提供了包含16个条目的专项规范。本文旨在对PRISMA-S进行系统性中...文献检索是系统评价(SR)的基石,但其报告质量长期存在不足,严重影响研究的透明度与可重复性。PRISMA-S(PRISMA for Searching)作为PRISMA 2020的扩展版本,为文献检索报告提供了包含16个条目的专项规范。本文旨在对PRISMA-S进行系统性中文解读,基于其原始发布文献,在逐条阐释其内涵与要求的基础上,进一步探讨其跨学科的普适性、作为“报告规范”的内在局限,并针对中文数据库等本土化应用挑战提出实践建议。本文旨在为国内学者、期刊编辑及审稿人提供清晰、实用的PRISMA-S应用指引,以期提升我国SR文献检索部分的报告质量,促进SR研究的规范化与国际化。展开更多
[背景]近年来,车载信息系统(In-vehicle Information System,IVIS)在商用车市场呈现快速普及态势。这种基于触摸屏的人机交互系统虽然提升了信息可视化程度,但同时也带来了不容忽视的驾驶分心问题,对行车安全构成了潜在威胁。[目标]探...[背景]近年来,车载信息系统(In-vehicle Information System,IVIS)在商用车市场呈现快速普及态势。这种基于触摸屏的人机交互系统虽然提升了信息可视化程度,但同时也带来了不容忽视的驾驶分心问题,对行车安全构成了潜在威胁。[目标]探究交互任务复杂度及人口统计学参数对驾驶员与IVIS界面交互时的认知负荷及行为表现的影响。[方法]使用受试者内-受试者间的重复测量实验设计,招募60名驾驶员开展驾驶模拟器实验。通过广义估计方程及其事后检验,探究不同自变量对驾驶员注视分配、转向熵、行驶速度、操作完成时间及主客观认知负荷的影响,并通过Python编程软件对驾驶员与IVIS交互时的注视行为进行可视化。[结果]复杂度高的任务会导致驾驶员的注意力分散,驾驶表现较差(转向熵、操作完成时间增大),主客观认知负荷也呈增高趋势;男性驾驶员的平均行驶速度比女性高9.45 km/h;年长组驾驶员(41~60岁)虽任务操作完成时间较长,但风险感知能力更高。[应用]可为未来智能汽车IVIS交互优化及个性化人机交互设计提供指导,进而提升人机交互可靠性。展开更多
Microseismic monitoring technology is widely used in tunnel and coal mine safety production.For signals generated by ultra-weak microseismic events,traditional sensors encounter limitations in terms of detection sensi...Microseismic monitoring technology is widely used in tunnel and coal mine safety production.For signals generated by ultra-weak microseismic events,traditional sensors encounter limitations in terms of detection sensitivity.Given the complex engineering environment,automatic multi-classification of microseismic data is highly required.In this study,we use acceleration sensors to collect signals and combine the improved Visual Geometry Group with a convolutional block attention module to obtain a new network structure,termed CNN_BAM,for automatic classification and identification of microseismic events.We use the dataset collected from the Hanjiang-to-Weihe River Diversion Project to train and validate the network model.Results show that the CNN_BAM model exhibits good feature extraction ability,achieving a recognition accuracy of 99.29%,surpassing all its counterparts.The stability and accuracy of the classification algorithm improve remarkably.In addition,through fine-tuning and migration to the Pan Ⅱ Mine Project,the network demonstrates reliable generalization performance.This outcome reflects its adaptability across different projects and promising application prospects.展开更多
OBJECTIVE:To investigate the mechanism of Dan Ze mixture(丹泽合剂,DZM)in the treatment of lipotoxic cardiomyopathy.METHODS:Ultra-performance liquid chromatography tandem mass spectrometry was employed to characterize ...OBJECTIVE:To investigate the mechanism of Dan Ze mixture(丹泽合剂,DZM)in the treatment of lipotoxic cardiomyopathy.METHODS:Ultra-performance liquid chromatography tandem mass spectrometry was employed to characterize the serum migration constituents of DZM.A lipotoxic cardiomyopathy rat model was established through high-fat diet and intervened by different doses of DZM.The cardiac function was assessed using echocardiography,and hematoxylin and eosin,oil red O,and Masson staining were conducted to evaluate morphological changes,lipid accumulation,and fibrosis in myocardial tissue.Serum myocardial enzyme activity,lipid levels,and lipid content of myocardial tissue were measured,while fluorescent staining and colorimetry were used to assess oxidation levels in myocardial tissue.Mitochondrial membrane potential was detected by 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanineiodide(JC-1).Transmission electron microscopy was employed to observe ultrastructure and mitochondrial structure changes in myocardial tissue.Fluorescence double staining and colocalization were utilized to observe the binding of autophagosomes and mitochondria,while immunohistochemical staining was used to detect the expression of mitophagy-related proteins.Terminal deoxynucleoitidyl transferase mediated nick end labeling staining was employed for the identification of apoptosis in myocardial tissue,while quantitative real-time reverse transcriptase polymerase chain reaction(q RT-PCR)and Western blot were utilized for the detection of apoptosis,B-cell lymphoma-2 adenovirus E1B 19 k Da-interacting protein 3(BNIP3)/mitophagy signaling pathway-related genes and proteins.In palmitic acid-induced Rat H9C2 cardiomyocytes(H9c2)cells,various cellular parameters including cell viability,lactate dehydrogenase release,apoptosis rate,oxidative stress level,mitochondrial structure and function,and mitophagy level were assessed after the treatment of DZM drug-containing serum for a duration of 24 h.The cellular expressions of BNIP3/mitophagy signaling pathway relevant genes and proteins were further evaluated using q RT-PCR and Western blot techniques.RESULTS:A total of 295 prototypes(e.g.,phenolic acids,quinones,terpenoids)were identified in serum of rats after oral administration of DZM.In vivo,DZM therapy has been shown to effectively enhance cardiac function,mitigate high-fat diet-induced myocardial structural damage and lipid accumulation.Furthermore,DZM has demonstrated the ability to reduce lipid levels,attenuate cell apoptosis,combat oxidative stress,enhance mitochondrial structure and function,and activate the BNIP3/mitophagy signaling pathway.Furthermore,the silencing of BNIP3 has been shown to exacerbate palmitic acid-induced damages in H9c2 cells,while inhibiting the BNIP3/mitophagy signaling pathway can mitigate the inhibitory effects of DZM on palmitic acidinduced apoptosis,lipid deposition and oxidative stress.CONCLUSION:This study presents preliminary evidence for the therapeutic efficacy of DZM on lipotoxic cardiomyopathy through the activating BNIP3/mitophagy signaling pathway.展开更多
摘要文献检索是系统评价(SR)的基石,但其报告质量长期存在不足,严重影响研究的透明度与可重复性。PRISMA-S(PRISMA for Searching)作为PRISMA 2020的扩展版本,为文献检索报告提供了包含16个条目的专项规范。本文旨在对PRISMA-S进行系统性中文解读,基于其原始发布文献,在逐条阐释其内涵与要求的基础上,进一步探讨其跨学科的普适性、作为“报告规范”的内在局限,并针对中文数据库等本土化应用挑战提出实践建议。本文旨在为国内学者、期刊编辑及审稿人提供清晰、实用的PRISMA-S应用指引,以期提升我国SR文献检索部分的报告质量,促进SR研究的规范化与国际化。
摘要[背景]近年来,车载信息系统(In-vehicle Information System,IVIS)在商用车市场呈现快速普及态势。这种基于触摸屏的人机交互系统虽然提升了信息可视化程度,但同时也带来了不容忽视的驾驶分心问题,对行车安全构成了潜在威胁。[目标]探究交互任务复杂度及人口统计学参数对驾驶员与IVIS界面交互时的认知负荷及行为表现的影响。[方法]使用受试者内-受试者间的重复测量实验设计,招募60名驾驶员开展驾驶模拟器实验。通过广义估计方程及其事后检验,探究不同自变量对驾驶员注视分配、转向熵、行驶速度、操作完成时间及主客观认知负荷的影响,并通过Python编程软件对驾驶员与IVIS交互时的注视行为进行可视化。[结果]复杂度高的任务会导致驾驶员的注意力分散,驾驶表现较差(转向熵、操作完成时间增大),主客观认知负荷也呈增高趋势;男性驾驶员的平均行驶速度比女性高9.45 km/h;年长组驾驶员(41~60岁)虽任务操作完成时间较长,但风险感知能力更高。[应用]可为未来智能汽车IVIS交互优化及个性化人机交互设计提供指导,进而提升人机交互可靠性。
基金supported by the Key Research and Development Plan of Anhui Province(202104a05020059)the Excellent Scientific Research and Innovation Team of Anhui Province(2022AH010003)support from Hefei Comprehensive National Science Center is highly appreciated.
摘要Microseismic monitoring technology is widely used in tunnel and coal mine safety production.For signals generated by ultra-weak microseismic events,traditional sensors encounter limitations in terms of detection sensitivity.Given the complex engineering environment,automatic multi-classification of microseismic data is highly required.In this study,we use acceleration sensors to collect signals and combine the improved Visual Geometry Group with a convolutional block attention module to obtain a new network structure,termed CNN_BAM,for automatic classification and identification of microseismic events.We use the dataset collected from the Hanjiang-to-Weihe River Diversion Project to train and validate the network model.Results show that the CNN_BAM model exhibits good feature extraction ability,achieving a recognition accuracy of 99.29%,surpassing all its counterparts.The stability and accuracy of the classification algorithm improve remarkably.In addition,through fine-tuning and migration to the Pan Ⅱ Mine Project,the network demonstrates reliable generalization performance.This outcome reflects its adaptability across different projects and promising application prospects.
基金Scientific Research Project of Hebei Province Administration of Traditional Chinese Medicine:to Explore the Protective Effect and Mechanism of Zexie Decoction on Lipotoxic Cardiomyopathy based on the p-mitogen-activated protein kinases/Peroxisome proliferator-activated receptorγcoactivator 1-alpha(p MAPK/PGC-1α)Signaling Pathway(No.2022096)Medical Science Research Project of Hebei Province:the Effect of 23-acetyl Alismol-B on Mitochondrial Function in Palmitic Acid-induced H9c2 Cells Was Investigated based on the Ca2+-Cyclic Adenosine Monophosphate(c AMP)-Response Element Binding Protein/c AMP Response Element(CREB/CRE)-PGC-1αSignaling Pathway(No.20221490)+1 种基金Hebei province natural science fund project:Study on the Mechanism of Danshen Zexie Decoction in Activating Nuclear Factor Erythroid 2-related Factor 2 Signaling Pathway to Trigger 0mi/Htr A2,Restoring Autophagic Flux and Enhancing Metabolism-Related Fatty Liver Disease(No.H2023423064)Hebei graduate student innovation ability funding training project:to Investigate the Protective Effects and Underlying Mechanisms of Zexie Decoction on Lipotoxic Cardiomyopathy,with A Focus on the PGC-1a Signaling Pathway(No.CXZZBS2022096)。
摘要OBJECTIVE:To investigate the mechanism of Dan Ze mixture(丹泽合剂,DZM)in the treatment of lipotoxic cardiomyopathy.METHODS:Ultra-performance liquid chromatography tandem mass spectrometry was employed to characterize the serum migration constituents of DZM.A lipotoxic cardiomyopathy rat model was established through high-fat diet and intervened by different doses of DZM.The cardiac function was assessed using echocardiography,and hematoxylin and eosin,oil red O,and Masson staining were conducted to evaluate morphological changes,lipid accumulation,and fibrosis in myocardial tissue.Serum myocardial enzyme activity,lipid levels,and lipid content of myocardial tissue were measured,while fluorescent staining and colorimetry were used to assess oxidation levels in myocardial tissue.Mitochondrial membrane potential was detected by 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanineiodide(JC-1).Transmission electron microscopy was employed to observe ultrastructure and mitochondrial structure changes in myocardial tissue.Fluorescence double staining and colocalization were utilized to observe the binding of autophagosomes and mitochondria,while immunohistochemical staining was used to detect the expression of mitophagy-related proteins.Terminal deoxynucleoitidyl transferase mediated nick end labeling staining was employed for the identification of apoptosis in myocardial tissue,while quantitative real-time reverse transcriptase polymerase chain reaction(q RT-PCR)and Western blot were utilized for the detection of apoptosis,B-cell lymphoma-2 adenovirus E1B 19 k Da-interacting protein 3(BNIP3)/mitophagy signaling pathway-related genes and proteins.In palmitic acid-induced Rat H9C2 cardiomyocytes(H9c2)cells,various cellular parameters including cell viability,lactate dehydrogenase release,apoptosis rate,oxidative stress level,mitochondrial structure and function,and mitophagy level were assessed after the treatment of DZM drug-containing serum for a duration of 24 h.The cellular expressions of BNIP3/mitophagy signaling pathway relevant genes and proteins were further evaluated using q RT-PCR and Western blot techniques.RESULTS:A total of 295 prototypes(e.g.,phenolic acids,quinones,terpenoids)were identified in serum of rats after oral administration of DZM.In vivo,DZM therapy has been shown to effectively enhance cardiac function,mitigate high-fat diet-induced myocardial structural damage and lipid accumulation.Furthermore,DZM has demonstrated the ability to reduce lipid levels,attenuate cell apoptosis,combat oxidative stress,enhance mitochondrial structure and function,and activate the BNIP3/mitophagy signaling pathway.Furthermore,the silencing of BNIP3 has been shown to exacerbate palmitic acid-induced damages in H9c2 cells,while inhibiting the BNIP3/mitophagy signaling pathway can mitigate the inhibitory effects of DZM on palmitic acidinduced apoptosis,lipid deposition and oxidative stress.CONCLUSION:This study presents preliminary evidence for the therapeutic efficacy of DZM on lipotoxic cardiomyopathy through the activating BNIP3/mitophagy signaling pathway.