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Niche vs.habitat:Insights of aging microplastics and wetland types on bacterial community assembly 认领 引用
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作者 Yansong Shi Longrui Liang +1 位作者 Liang Meng Jingwen Hou 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第1期221-232,共12页
Microorganisms can colonize the surface of microplastics(MPs)to form a distinctive microbiome,known as a“plastisphere”which is regarded as an anthropogenic niche for microbial growth.However,bacterial community asse... Microorganisms can colonize the surface of microplastics(MPs)to form a distinctive microbiome,known as a“plastisphere”which is regarded as an anthropogenic niche for microbial growth.However,bacterial community assembly in virgin and aging MP plastispheres across different habitats is poorly understood.This study aims to assess the variations in bacterial community assembly across different niches and habitats with an in situ ex-periment,in which constructed forest wetland(FW),natural lake wetland(LW),and lotus pond wetland(LP)were habitats,and plastispheres of virgin and aging low-density polyethylene(LDPE)MPs,as well as surround-ing wetland soils were niches.Significant niche-related differences in bacterial communities were observed,with lower diversity and enrichment of potential plastic-degrading bacteria in the plastisphere than in the soil bacterial communities.Furthermore,habitat-related differences exerted a more pronounced influence on the beta-diversity patterns of the bacterial communities.The linear regression analyses indicated that the local species pool con-tributed more to bacterial community assembly in the LW wetland,whereas the relative abundance of species was the primary factor in the LP wetland.The null model analysis indicated that plastisphere bacterial communi-ties were predominantly driven by the stochastic process,with a more deterministic assembly observed in the LP wetland and soil bacterial communities.Additionally,the primary ecological process shaping plastisphere com-munities shifted from drift in the virgin LDPE to homogenising dispersal in the aging LDPE.This study provides new insights into the fate and ecological impacts of MPs in wetlands,thereby facilitating the effective regulations of plastic pollution. 展开更多
关键词 Plastisphere Community assembly mechanism Local species pool Stochastic assembly Homogeneous process
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Carbon Dot Self-Assembly:Bottom-Up Fabrication,Morphological Evolution,Luminescence Regulation,and Advanced Applications 认领 引用
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作者 Kai Chen Zhi-Min Zhao +3 位作者 Zhi-Hua Liu Li Xu Junbo Gong Bing-Zhi Li 《Aggregate》 EI CAS CSCD 2026年第5期85-111,共27页
Carbon dots(CDs)have garnered widespread interest in biosensing,optoelectronics,and biomedicine due to their exceptional fluorescence,biocompatibility,and low cost.While individual CDs exhibit tunable properties,their... Carbon dots(CDs)have garnered widespread interest in biosensing,optoelectronics,and biomedicine due to their exceptional fluorescence,biocompatibility,and low cost.While individual CDs exhibit tunable properties,their organization into higher-order architectures via self-assembly enables emergent collective functionalities that are inaccessible to discrete nanoparticles.This review presents a systematic overview of the design principles and recent advances in CD self-assembly.We first summarize synthetic strategies and the morphological evolution of assemblies,spanning zero-dimensional nanoparticles to three-dimensional macroscopic materials.We then discuss how self-assembly modulates photophysical properties,followed by an analysis of the underlying supramolecular mechanisms.Subsequently,representative applications enabled by these assemblies are highlighted.Finally,we outline key challenges related to scalability,stability,and the prediction of structure-property relationships,and provide an outlook on leveraging self-assembly to fully realize the potential of CDs for advanced functional materials. 展开更多
关键词 assembly mechanism carbon dots circularly polarized luminescence morphological control self-assembly
Chromosome-level genome assembly and population genomics analysis of Camellia rubituberculata provide insights into adaptation to karst habitats 认领 引用 被引量：2
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作者 Chao Yan Ming-tai An +4 位作者 Ming Tang Xin-xiang Bai Xu Xiao Zhao-hui Ran Zhi Li 《Plant Diversity》 SCIE CAS CSCD 2026年第2期246-261,共16页
Karst flora confined to isolated‘habitat islands'evolve specialized adaptations and unique traits,serving as ideal models for investigating adaptive evolution and species diversification mechanisms.Camellia rubit... Karst flora confined to isolated‘habitat islands'evolve specialized adaptations and unique traits,serving as ideal models for investigating adaptive evolution and species diversification mechanisms.Camellia rubituberculata,endemic to the karst habitats of Guizhou,China,can serve as a model for adaptive evolution and diversity in karst-endemic woody species.However,the lack of a chromosome-level genome for this species has limited in-depth studies on its adaptations to karst and posed a barrier to its genetic improvement.In this study,a chromosome-level genome assembly of C.rubituberculata was generated,with 15 pseudo-chromosomes and a genome size of 2.50 Gb(scaffold N50=168.34 Mb,55,302 protein-coding genes).Comparative genomics revealed two whole-genome duplications(WGDs),namely,an ancient γ-event(~120 Mya)and a subsequent genus-wide event(~86 Mya),after which gene families linked to karst adaptation(e.g.,photosynthesis)were significantly expanded.Selective sweep analysis showed that selected genes were associated with phytohormone transmission and metabolism.Genes functionally annotated as involved in stress responses—including SAUR,BSK,NCL,CDPK,and NDPK—participate in calcium homeostasis and ion transport pathways under karstspecific stresses.MYB transcription factors,which are crucial in plant responses to stresses,including drought,may be key for adaptation to the high salinity and drought stress in karst environments.The divergent selection in wild and cultivated groups highlight key adaptations in plant hormone transduction and calcium transport.By elucidating karst adaptation in C.rubituberculata,this work establishes essential genomic resources for advancing genetic evolution research and molecular breeding across Camellia species. 展开更多
关键词 Camellia rubituberculata Endemic species Genome assembly Karst adaptation Population genetics
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Complete genome assembly of the Xian rice variety IR64 as a valuable source in genomics and breeding research 认领 引用 被引量：1
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作者 Min Li Tingting Sheng +13 位作者 Linjun Yu Shuyue Zheng Shijiao Li Shuran Zhou Fengcai Wu Fan Zhang Chaopu Zhang Erbao Liu Yingyao Shi Xue Mi Xueru Zhang Zhikang Li Yanru Cui Wensheng Wang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2026年第3期551-554,共4页
IR64 is an elite Xian/indica variety developed by International Rice Research Institute(IRRl)in 1985,which has been the most widely grown variety and core breeding parent in South/Southeast Asia(Mackill and Khush,2018... IR64 is an elite Xian/indica variety developed by International Rice Research Institute(IRRl)in 1985,which has been the most widely grown variety and core breeding parent in South/Southeast Asia(Mackill and Khush,2018).IR64 has been utilized to develop stress-tolerant(such as drought-adapted and submergenceresistant)near-isogenic lines,underscoring its great potential in agricultural genomics(Tanaka et al.,2020). 展开更多
关键词 Xian rice IR core breeding parent breeding research agricultural genomics tanaka genomics genome assembly stress tolerance
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Environmental DNA reveals multi-trophic biodiversity patterns and community assembly mechanisms across contrasting river systems on the Qinghai-Xizang Plateau 认领 引用 被引量：1
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作者 Qi Lu Si-Yu Zhang +2 位作者 Jianqing Du Yongqin Liu Meng Yao 《Journal of Systematics and Evolution》 SCIE CSCD 2026年第1期138-155,共18页
The Hindu Kush Himalayan(HKH)region sustains the headwaters of major Asian rivers and harbors unique alpine biodiversity,and yet,it is highly sensitive to climate change.Biodiversity organization and community assembl... The Hindu Kush Himalayan(HKH)region sustains the headwaters of major Asian rivers and harbors unique alpine biodiversity,and yet,it is highly sensitive to climate change.Biodiversity organization and community assembly processes across trophic levels within its river ecosystems remain poorly understood.Here,we used multi-marker environmental DNA(eDNA)metabarcoding targeting four biological groups(cyanobacteria,diatoms,invertebrates,and vertebrates)to assess multi-trophic biodiversity patterns in two alpine rivers of the region:the Yellow River source region(YR)and the middle-upper Nujiang River(NJ).These two systems differ markedly in geography,hydroclimate,vegetation,and human disturbance intensity.We identified 1695 operational taxonomic units and revealed pronounced differences in biodiversity pattern and community composition between rivers.Phototrophs and invertebrates showed higherαdiversity in NJ,whereas vertebrates were richer in YR.βdiversity was mainly driven by species turnover in both rivers,with a stronger distance-decay pattern in YR.Correlations between environmental variables andαandβdiversity varied across groups and rivers,with geographic and climatic factors exerting stronger effects in NJ.iCAMP and pNST analyses revealed that stochastic processes dominated community assembly in both rivers,whereas deterministic processes were relatively stronger in YR compared to NJ.Accordingly,co-occurrence networks revealed cohesive communities in YR but more modular ones in NJ,indicating contrasting ecological stability regimes.Overall,our study provides an integrated,multi-trophic perspective on how environmental gradients shape riverine biodiversity and ecological interactions,informing adaptive conservation strategies under accelerating environmental change in the HKH region. 展开更多
关键词 alpine freshwater biodiversity community assembly ecological networks hydroclimatic gradients
A Fully-Actuated Pose Adjustment Method for Wing-Fuselage Assembly via Point-Cloud Gap Optimization 认领 引用
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作者 Xiao Xu Yang Zhang +4 位作者 Runze Liu Haoyu Wu Qihang Chen Yongkang Lu Wei Liu 《Instrumentation》 2026年第2期109-124,共16页
Wing-fuselage assembly is a critical process in aircraft manufacturing,and the gap distribution of the wing-fuselage assembly frames directly determines the stress state of the connection interface and the structural ... Wing-fuselage assembly is a critical process in aircraft manufacturing,and the gap distribution of the wing-fuselage assembly frames directly determines the stress state of the connection interface and the structural load-carrying capacity after assembly.However,most existing pose adjustment methods either do not consider the global gap distribution or optimize the gap only based on theoretical computer-aided design(CAD)models,making it difficult to effectively control the actual gap distribution under real manufacturing errors.In addition,traditional positioners adopt a master-slave driving mode,in which passive axes suffer from following errors due to the lack of independent control,thereby limiting the execution accuracy of pose adjustment.To address these problems,this paper proposes a fullyactuated pose adjustment method for wing-fuselage assembly via point-cloud gap optimization.First,point cloud data of the wing-fuselage assembly frames in a unified assembly coordinate system are obtained through laser scanning combined with Enhanced Reference System(ERS)reference point registration.Assembly gaps are then defined along prescribed region-wise assembly directions,and a multi-surface global gap evaluation model is established.Second,a two-stage optimization strategy combining coarse pre-alignment and fine pose adjustment is adopted,in which insertion-depth correction is decoupled from rotational gap-uniformity optimization to solve the optimal target assembly pose.Finally,the optimized pose is converted into multi-axis synchronous motion commands for fully-actuated positioners through fifth-order polynomial trajectory planning and inverse kinematic mapping,and the force-position data of the positioners are effectively monitored throughout the pose adjustment process.The proposed method is compared with a traditional manual assembly method on a wing-fuselage assembly experimental platform.The results show that the proposed method reduces the in-plane gap standard deviations of the upper and right assembly surfaces from 2.19 mm and 1.34 mm to 1.70 mm and 1.04 mm,respectively,significantly improving gap uniformity.Meanwhile,the positioner motion remains continuous and smooth during pose adjustment,and no abnormal force fluctuations occur at the support points.The proposed method provides an integrated and executable solution for highprecision wing-fuselage assembly by linking measured-point-cloud-based global gap optimization,fully-actuated pose adjustment,and real-time force-position monitoring,thereby supporting assembly safety assessment and quality traceability. 展开更多
关键词 wing-fuselage assembly 3D point cloud measurement assembly gap pose optimization fully-actuated pose adjustment
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Rules Governing General Assembly of Microbial Communities in Engineered Biotreatment Processes 认领 引用
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作者 Yong-Chao Wang Ya-Hui Lv +4 位作者 Ye Deng Yu-Ting Lin Guan-Yu Jiang John C.Crittenden Can Wang 《Engineering》 SCIE EI CSCD 2026年第5期260-269,共10页
The principles of microbial community assembly in engineered ecosystems(particularly biotreatment processes)exposed to high-intensity environmental disturbances remain poorly understood.In this study,we conducted a me... The principles of microbial community assembly in engineered ecosystems(particularly biotreatment processes)exposed to high-intensity environmental disturbances remain poorly understood.In this study,we conducted a meta-analysis of microbial communities across nine datasets from bioreactors operated under varied conditions.Null model-based analysis indicated that stochastic processes predominantly govern community assembly during contaminant-free selection phases or stable operation.In contrast,deterministic processes consistently govern initial acclimation of activated sludge.These processes then shift toward stochastic dominance as the operation stabilizes.The presence of environmental disturbances—such as shock,refeeding,or stress—during operation increases the relative contribution of deterministic processes to community structuring.Notably,low bioreactor performance is associated with deterministic assembly,whereas sustained stable operation corresponds to stochastic dynamics,irrespective of reactor type or condition.These findings were integrated across four categories of biotreatment operations to illustrate how specific disturbances influence microbial community succession and performance stability.This work advances the theoretical understanding of microbial dynamics in engineered systems and offers practical insights into linking community assembly mechanisms with bioreactor function. 展开更多
关键词 Microbial community assembly Engineered ecosystems Environmental disturbances Performance Operation stability
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Insight into the differences in airborne bioaerosols dynamic assembly between hospital and school environments in summer 认领 引用
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作者 Lili Wang Yupeng He +4 位作者 Xueying Li Xin Liu Li Chen Kangbo Tong Yang Yun 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第3期436-444,共9页
Hospital-influenced bioaerosols pose critical health risks due to their pathogenic potential,yet their dynamic assembly mechanisms during vulnerable time period remain poorly characterized.Through parallel sampling of... Hospital-influenced bioaerosols pose critical health risks due to their pathogenic potential,yet their dynamic assembly mechanisms during vulnerable time period remain poorly characterized.Through parallel sampling of hospital-and school-influenced bioaerosol during summer periods,we revealed distinct diurnal fluctuations and site-specific microbial assembly patterns.HYSPLIT trajectory modeling demonstrated that school-influenced bioaerosols exhibited greater meteorological source diversity than hospital counterparts,promoting stochastic assembly processes.Conversely,co-occurrence network analysis identified deterministic assembly driven by hospital bioaerosols capacity to utilize particulate-bound polycyclic aromatic hydrocarbons(PAHs).Notably,Proteobacteria-associated pathogens exhibited environmental resilience and were significantly more abundant in hospital aerosols compared to school environments.These findings challenge the conventional view of bioaerosols as passively dispersed entities,establishing a mechanistic link between anthropogenic pollution sources and pathogenic bioaerosol persistence in summer ecosystems.Our work provides critical insights for predicting microbial risks in healthcare-associated atmospheric environments. 展开更多
关键词 Bioaerosol Community assembly Component utilization Polycyclic aromatic hydrocarbons
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An efficientself-adaptive planning algorithm for shield machine attitude and segment assembly considering machine-segment interaction 认领 引用
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作者 Gan Wang Qian Fang +3 位作者 Jun Wang Zhenhua Ru Jianying Wei Jinkun Huang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2026年第7期5109-5124,共16页
Both shield machine attitude and segment assembly play crucial roles in ensuring the construction quality of shield tunnels.This study proposes an efficientSelf-Adaptive Planning Algorithm(SAPA)for shield machine atti... Both shield machine attitude and segment assembly play crucial roles in ensuring the construction quality of shield tunnels.This study proposes an efficientSelf-Adaptive Planning Algorithm(SAPA)for shield machine attitude control and segment assembly,which jointly considers shield machine attitude planning and segment assembly point selection.A series of efficientthree-dimensional(3D)computational methods is developed for multiple key indicators,including distance deviation,angular deviation,cylinder stroke difference,tail clearance,and stagger-jointed assembly.With the developed selfadaptive weighting method,SAPA achieves a balanced optimization of all indicators,ensuring compliance with all control criteria during shield tunneling.Importantly,SAPA fully accounts for the interaction between the shield machine and segments during shield advancement and segment assembly.A series of analyses reveals that SAPA significantlyoutperforms the traditional fixed-weight method,primarily due to the self-adaptive weighting method.Among the evaluated optimization algorithms,Broyden-Fletcher-Goldfarb-Shanno(BFGS)algorithm demonstrates the best overall performance while satisfying engineering computational speed constraints.As the calculation interval increases,the overall performance of SAPA declines,while computational time decreases exponentially.A calculation interval of 0.1 m is recommended as it provides a favorable balance between accuracy and efficiency.Compared to the traditional trajectory correction method,SAPA enables fully autonomous trajectory correction with greater efficiency,effectively avoiding over-correction and reducing labor costs. 展开更多
关键词 Shield tunnel Shield machine attitude Segment assembly Self-adaptive Optimization
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Effect of electrostatic assembly of silver nanoparticle on microstructure and properties of laser additive manufactured TC4-ELI biomaterials 认领 引用
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作者 Yan-bing GUO Xin TANG +3 位作者 Zhi-kang SHEN Zhi-xi ZHANG Kai FENG Li ZENG 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2026年第5期1508-1526,共19页
The incorporation of 2 wt.% silver(Ag)nanoparticles into TC4-ELI titanium alloy via electrostatic assembly was investigated,followed by laser powder bed fusion additive manufacturing.The objective was to enhance the a... The incorporation of 2 wt.% silver(Ag)nanoparticles into TC4-ELI titanium alloy via electrostatic assembly was investigated,followed by laser powder bed fusion additive manufacturing.The objective was to enhance the alloy’s mechanical properties,corrosion resistance,and antibacterial performance to test its potential application in body implants.The results show that electrostatically assembled TC4-ELI/Ag samples achieve a relative density of 99.5%,an ultimate tensile strength of 1250.3 MPa,and an elongation of 16.13%,significantly surpassing unmodified TC4-ELI and ball-milled TC4-ELI/Ag samples.The electrochemical analysis confirms enhanced corrosion resistance,with TAM3 samples(electrostatically assembled)exhibiting superior passivation and a lower corrosion current density of 0.239μA/cm².Antibacterial tests demonstrate the best performance for TAM3,with a marked reduction in bacterial growth attributed to the uniform dispersion and controlled release of Ag ions. 展开更多
关键词 TC4-ELI/Ag nanocomposites electrostatic assembly microstructure mechanical properties corrosion resistance antibacterial activity against E.coli
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Chromosome-level genome assembly of the endangered Magnolia sinostellata:Insights into genome evolution,cold stress resistance,and terpenoid biosynthesis 认领 引用
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作者 Huijuan Zhou Fan Wu +12 位作者 Hengzhao Liu Jiayu Ma Huiling Yan Renna Li Lu Fan Fangbing Ding Yuwei Linghu Bin Xie Xiaoai Fang Shu Yang Ming Yue Peng Zhao Yaling Wang 《Journal of Systematics and Evolution》 SCIE CSCD 2026年第2期260-282,共23页
The genus Magnolia belongs to Magnoliaceae,an early diverging lineage of the Magnoliales,and is cultivated globally for its high ornamental and commercial values.As a large genus in the family Magnoliaceae,Magnolia sp... The genus Magnolia belongs to Magnoliaceae,an early diverging lineage of the Magnoliales,and is cultivated globally for its high ornamental and commercial values.As a large genus in the family Magnoliaceae,Magnolia species are regarded as highly valuable in phylogenetic and conservation biological studies.However,the whole genome data of Magnolia is still relatively insufficient.Here,we present a high-quality,chromosome-level genome sequence of Magnolia sinostellata(1.86 Gb)with a scaffold N50 of 85.33 Mb.The 19 M.sinostellata genome chromosomes revealed 11 main duplications representing the subgenome.Comparative genomics analysis revealed that the variance in the number of abiotic stress resistance genes among Magnoliid species are related to different environmental adaptations.Most of the genes related to MAPK signaling and stress resistance pathways in the investigated M.sinostellata species are expanded,compared to the other species.Furthermore,the comparative genomics analysis of three Magnolia assemblies,M.sinostellata,Magnolia biondii,and Magnolia sieboldii revealed that large inversions were enriched in terpenoid metabolic pathways,stress resistance and flavonoid biosynthesis,and DNA replication proteins.Using transcriptome sequencing data,we analyzed the expression levels of genes related to terpenoid biosynthesis(terpene synthase)and ICE-CBF-COR gene models related to cold tolerance in various tissues and the buds under different temperature conditions.The high-quality assembly of M.sinostellata and the ICE-CBF-COR bioinformatic analysis cascade provide valuable resources for studying the phylogeny and evolution of Magnoliaceae and angiosperms,while the candidate genes will provide foundational support for molecular breeding in Magnolia species. 展开更多
关键词 chromosome duplication chromosome-scale genome assembly comparative genome ICE-CBF-COR signaling pathway Magnolia sinostellata terpenoid biosynthesis
Impact of organic carbon composition on bacterial community assembly in organic and mineral soil layers under phosphorus addition in a Robinia pseudoacacia plantation 认领 引用
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作者 Ahejiang SAILIKE Yujie LIANG +10 位作者 Rong FU Hongjian HAO Rong WANG Ning PENG Shicai LI Zhouchang YU Fangxin ZHENG Wei ZHANG Yangyang LIU Peizhi YANG Zhixin ZHANG 《Pedosphere》 SCIE CAS CSCD 2026年第2期554-567,共14页
Increasing phosphorus(P)inputs significantly affect the nutrient cycle of terrestrial ecosystems.The community structure and assembly processes of soil bacteria,which are the primary participants in nutrient cycling w... Increasing phosphorus(P)inputs significantly affect the nutrient cycle of terrestrial ecosystems.The community structure and assembly processes of soil bacteria,which are the primary participants in nutrient cycling within terrestrial ecosystems,are affected by P inputs.However,the assembly processes and driving mechanisms of soil bacterial communities in artificial forest ecosystems in response to P inputs remain unclear.Therefore,in this study,an experiment was conducted in an artificial Robinia pseudoacacia forest on the Loess Plateau to investigate the effects of P additions(0,0.5,1,2,and 4 g P m-2year-1)on soil organic carbon(SOC)composition,bacterial community characteristics(total,abundant,and rare taxa),and assembly processes in the organic horizon(OH)and mineral horizon(MH).The results showed that P addition significantly changed soil physicochemical properties,such as available P(AP),SOC content,and SOC composition in terms of labile and recalcitrant SOC fractions.The proportion of labile SOC fraction increased in OH and decreased in MH,whereas the recalcitrant SOC fraction showed the opposite trend.In OH,the proportion of eutrophic bacteria increased,whereas the proportion of oligotrophic bacteria decreased.In contrast to those in OH,the changes in eutrophic and oligotrophic bacteria in MH exhibited the opposite trend.The diversity of bacterial communities in OH did not change significantly with increasing P levels.In MH,the diversity of total and rare taxa increased,while the diversity of abundant taxa decreased.Under P addition,the total and rare bacterial taxa showed stochastic assembly,whereas the assembly of abundant taxa shifted from stochastic to deterministic processes in OH and from deterministic to stochastic processes in MH.The partial-least squares path modeling revealed that under P addition,the assembly of total and rare bacterial communities in OH and MH was regulated by AP and labile SOC fraction,respectively.Meanwhile,the assembly of abundant taxa in OH and MH was influenced by labile and recalcitrant SOC fractions,respectively.Our findings highlight the critical role of SOC composition across different bacterial taxa,with abundant taxa exhibiting heightened sensitivity to environmental filtration and the availability of SOC resources.Our study provides new insights into the community assembly and driving mechanisms of soil bacteria in artificial forest soils under P addition. 展开更多
关键词 abundant taxa afforestation artificial forest bacterial community assembly mineral horizon organic horizon rare taxa soil organic C
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Genomic assembly,rescue,and characterization of a functional pseudorabies virus 认领 引用
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作者 Hengrui Hu Xiquan Ke +7 位作者 Han Xiao Xianfei Shang Qiran Yin Jiang Li Xiangmin Li Zhihong Hu Ping Qian Manli Wang 《Virologica Sinica》 SCIE CAS CSCD 2026年第1期97-106,共10页
With recent advances in synthetic biology methods,the genomes of several large DNA viruses have been de novo synthesized and assembled,leading to the functional rescue of the respective viruses.Pseudorabies virus(PRV)... With recent advances in synthetic biology methods,the genomes of several large DNA viruses have been de novo synthesized and assembled,leading to the functional rescue of the respective viruses.Pseudorabies virus(PRV),a large DNA virus belonging to the family Herpesviridae,causes severe diseases in swine,resulting in significant economic losses to the global pig farming industry.Genome editing is crucial for attenuating virulence and developing safer vaccines for PRV.However,its complex repetitive sequences and extremely high GC-rich genome pose significant challenges for genetic manipulation.In this study,we developed a PRV genome assembly platform using yeast-based transformation-associated recombination(TAR)technology.The genome of a prevalent genotype Ⅱ variant strain,PRV-GX-2011(GenBank number PV405324.1),was divided into nine A-level fragments and cloned into vectors via TAR.Subsequently,three B-level fragments were generated by recombining three A-level fragments each.In vitro CRISPR/Cas9-mediated editing was introduced to insert an egfp gene into the non-coding intergenic region between UL23 and UL22 genes.Infectious viruses were rescued by co-transfection of linearized B-level fragments in Vero cells,and an isolated virus,PRV-GX-Syn1,was purified via plaque assay.While PRV-GX-Syn1 exhibited reduced viral titer and smaller plaque size compared to the parental strain,its morphological characteristics remained indistinguishable from the parental virus.In BALB/c mice,PRV-GX-Syn1 caused lethal infection,producing lung pathology comparable to the parental strain.This TAR-based platform offers faster and more flexible genomic modification of PRV,facilitating both basic research and PRV-based vaccine vectors. 展开更多
关键词 Pseudorabies virus(PRV) Genome assembly TAR CRISPR/Cas9 Attenuated vaccine vector Virus rescue
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Achieving photo-controlled reversible multicolor luminescent supramolecular assembly by multicharged β-cyclodextrin 认领 引用
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作者 Rong Zhang Zhiyi Yu +1 位作者 Hengyi Zhang Yu Liu 《Chinese Chemical Letters》 SCIE CAS CSCD 2026年第6期489-496,共8页
Multivalent supramolecular assemblies not only can efficiently enhance the photophysical properties of functional molecules,but also are able to intelligently modulate the luminescence behavior.Herein,the photo-contro... Multivalent supramolecular assemblies not only can efficiently enhance the photophysical properties of functional molecules,but also are able to intelligently modulate the luminescence behavior.Herein,the photo-controlled reversible multicolor luminescent supramolecular assembly was constructed on agarose substrate by non-covalent interactions,which was composed of multichargedβ-cyclodextrin(AMCD)with adamantane-modified spiropyrane derivative(Adam-SP)and tetraphenylethene derivatives(TPE).Firstly,the positively charged AMCD and negatively charged TPE form a binary assembly AMCD@TPE through electrostatic interaction,significantly enhancing the luminescence of TPE at 480 nm with a quantum yield(QY)jump from 0.68%to 31.24%.Moreover,the binary assembly AMCD@TPE and Adam-SP further formed a ternary assembly Adam-MC@AMCD@TPE through strong host-guest interaction,which not only achieved photo-regulated multicolor reversible fluorescence in both aqueous solution and agarose hydrogel under alternating visible light irradiation and dark treatment,but also produced adjustable luminescence changes under thermal stimulation.The ternary assembly Adam-MC@AMCD@TPE was successfully applied to photo-controlled multiple anti-counterfeiting. 展开更多
关键词 Supramolecular assembly Multicharged cyclodextrin Multicolor luminescence Photo-responsiveness Dynamic anti-counterfeiting
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Deep Learning-Based Toolkit Inspection:Object Detection and Segmentation in Assembly Lines 认领 引用
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作者 Arvind Mukundan Riya Karmakar +1 位作者 Devansh Gupta Hsiang-Chen Wang 《Computers, Materials & Continua》 SCIE EI 2026年第1期1255-1277,共23页
Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone t... Modern manufacturing processes have become more reliant on automation because of the accelerated transition from Industry 3.0 to Industry 4.0.Manual inspection of products on assembly lines remains inefficient,prone to errors and lacks consistency,emphasizing the need for a reliable and automated inspection system.Leveraging both object detection and image segmentation approaches,this research proposes a vision-based solution for the detection of various kinds of tools in the toolkit using deep learning(DL)models.Two Intel RealSense D455f depth cameras were arranged in a top down configuration to capture both RGB and depth images of the toolkits.After applying multiple constraints and enhancing them through preprocessing and augmentation,a dataset consisting of 3300 annotated RGB-D photos was generated.Several DL models were selected through a comprehensive assessment of mean Average Precision(mAP),precision-recall equilibrium,inference latency(target≥30 FPS),and computational burden,resulting in a preference for YOLO and Region-based Convolutional Neural Networks(R-CNN)variants over ViT-based models due to the latter’s increased latency and resource requirements.YOLOV5,YOLOV8,YOLOV11,Faster R-CNN,and Mask R-CNN were trained on the annotated dataset and evaluated using key performance metrics(Recall,Accuracy,F1-score,and Precision).YOLOV11 demonstrated balanced excellence with 93.0%precision,89.9%recall,and a 90.6%F1-score in object detection,as well as 96.9%precision,95.3%recall,and a 96.5%F1-score in instance segmentation with an average inference time of 25 ms per frame(≈40 FPS),demonstrating real-time performance.Leveraging these results,a YOLOV11-based windows application was successfully deployed in a real-time assembly line environment,where it accurately processed live video streams to detect and segment tools within toolkits,demonstrating its practical effectiveness in industrial automation.The application is capable of precisely measuring socket dimensions by utilising edge detection techniques on YOLOv11 segmentation masks,in addition to detection and segmentation.This makes it possible to do specification-level quality control right on the assembly line,which improves the ability to examine things in real time.The implementation is a big step forward for intelligent manufacturing in the Industry 4.0 paradigm.It provides a scalable,efficient,and accurate way to do automated inspection and dimensional verification activities. 展开更多
关键词 Tool detection image segmentation object detection assembly line automation Industry 4.0 Intel RealSense deep learning toolkit verification RGB-D imaging quality assurance
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Dynamic assembly stabilizes buried 2D perovskite 认领 引用
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作者 Liyan Yang Tao Wang 《Science China Materials》 SCIE EI CAS CSCD 2026年第7期4309-4310,共2页
Perovskite solar cells(PSCs)have emerged as one of the leading candidates in third-generation photovoltaic technology.The perovskite film with uniform crystallization[1],from the top interface,through the bulk,and dow... Perovskite solar cells(PSCs)have emerged as one of the leading candidates in third-generation photovoltaic technology.The perovskite film with uniform crystallization[1],from the top interface,through the bulk,and down to the buried interface,is a premise for PSCs to achieve high efficiency and long-term stability.For the top interface[2]and bulk[3],strategies such as additive,interface modification,and strain homogenization have been proven effective in regulating the crystallization behavior of perovskite.However,the buried interface presents a grand challenge. 展开更多
关键词 perovskite solar cells pscs perovskite solar cells dynamic assembly buried interface crystallization behavior additiveinterface modificationand strain homogenization stability
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Telomere-to-telomere,gap-free assembly of the Rosa rugosa reference genome 认领 引用 被引量：2
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作者 Dan Liu Kun Liu +8 位作者 Boqiang Tong Haili Guo Kai Qu Ting Xu Rengang Zhang Wei Zhao Xiaoman Xie Longxin Wang Kaihua Jia 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第6期1450-1464,共15页
Rosa,a genus esteemed worldwide for its ornamental plants,has encountered barriers in functional genomic studies and further genetic enhancement due to incomplete sequences and floating regions in previously sequenced... Rosa,a genus esteemed worldwide for its ornamental plants,has encountered barriers in functional genomic studies and further genetic enhancement due to incomplete sequences and floating regions in previously sequenced genomes.Our groundbreaking study introduced a meticulously assembled,continuous,and fully bridged reference genome for Rosa rugosa,constructed through a sophisticated combination of PacBio High-Fidelity,ONT ultra-long reads,and Hi-C data.This robust assembly spanned 444.55 Mb and encompassed 34109 protein-coding genes.We have uniquely assembled each chromosome into single,gap-free structures,successfully identifying all 14 telomeres and seven centromeres,a feat not achieved previously.The centromeric regions were distinguished by tandem repeats,primarily composed of centromere-specific 159-bp monomers,and a significant enrichment of ATHILA/Gypsy long terminal repeat retrotransposons in proximal regions.Our research highlighted recent tandem duplications as instrumental in bolstering R.rugosa's stress tolerance,environmental adaptability,and enhanced anthocyanin synthesis.Furthermore,our study ventured into uncharted territory by predicting transcription factors potentially regulating anthocyanin biosynthesis through the employment of gene co-expression networks,providing new avenues for research.This comprehensive reference genome not only serves as a cornerstone for in-depth exploration of genomic architecture and functionalities in R.rugosa but also acts as a catalyst for innovative breeding strategies and genetic refinement within the genus. 展开更多
关键词 Rosa Telomere-to-telomere genome assembly Anthocyanin biosynthesis
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Assembly and integration of micro-led displays:a review of transfer methods targeting near-perfect yield 认领 引用 被引量：1
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作者 Seong Woo Hong Hyeon Ho Shin +5 位作者 Seol Ahn Hyeon Ji Yang Kyung Jun Lee Myungwon Lee Ji Woong Cho Yei Hwan Jung 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2026年第2期284-314,共31页
Micro-light-emitting diodes(micro-LEDs)are widely recognized for their superior brightness,efficiency,and durability,offering transformative potential for next-generation displays and emerging applications.However,the... Micro-light-emitting diodes(micro-LEDs)are widely recognized for their superior brightness,efficiency,and durability,offering transformative potential for next-generation displays and emerging applications.However,the path to commercialization remains hindered by a critical barrier:achieving an extremely high production yield.Unlike conventional displays,micro-LED displays require the precise transfer of millions of individual micro-LED chips,even the slightest defects can significantly affect the overall yield.This review focuses on the technological challenges of pushing micro-LED assembly yield to extremely high thresholds of 99.99999%.We explore six key transfer methods—elastomeric transfer,roll-to-roll printing,electrostatic and electromagnetic assembly,laser transfer,microvacuum assembly,and fluidic self-assembly—and analyze their respective impacts on yield.Recent advancements in each method are discussed,with an emphasis on strategies to overcome yield challenges.By framing yield as the central metric and not as a side concern,this review aims to provide a roadmap for overcoming bottlenecks in micro-LED assembly and enabling industrial-scale deployment. 展开更多
关键词 assembly integration micro-led displays transfer method yield
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Effects of host niche and genotype on the diversity and community assembly of the fungal community in peas(Pisum sativum L.) 认领 引用 被引量：1
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作者 Yu Wang Linying Xu +7 位作者 Liquan Zhang Rui Zhang Qiong Liu Hongquan Liu Tao Yang Haoqing Zhang Tida Ge Li Wang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第2期529-539,共11页
Fungi play crucial roles in nutrient acquisition,plant growth promotion,and the enhancement of resistance to both abiotic and biotic stresses.However,studies on the fungal communities associated with peas (Pisum sativ... Fungi play crucial roles in nutrient acquisition,plant growth promotion,and the enhancement of resistance to both abiotic and biotic stresses.However,studies on the fungal communities associated with peas (Pisum sativum L.) remain limited.In this study,we systematically investigated the ecological effects of host niches (soil,root,stem,leaf,and pod) and genotypes on the diversity and composition of fungal communities in peas using a multi-level approach that encompassed pattern recognition (β-diversity decomposition),mechanism validation (neutral community model testing),and dynamic tracking methods (migration pathway source-tracking).The results revealed that the dominant fungal phyla across niches and genotypes were Ascomycota,Basidiomycota,and Mortierellomycota,and the community structures of the soil–plant continuum were primarily determined by the pea niches rather than genotypes.β-diversity decomposition was largely attributed to species replacement rather than richness differences,indicating strong niche specificity and microbial replacement across microhabitats.Neutral model analysis revealed that stochastic processes influenced genotypeassociated communities,while deterministic processes played a dominant role in niche-based community assembly.Source-tracking analysis identified niche-to-niche fungal migration,with Erysiphe,Fusarium,Cephaliophora,Ascobolus,Alternaria,and Aspergillus as the key genera.Migration rates from exogenous to endogenous niches were low (1.3–61.5%),whereas those within exogenous (64.4–83.7%) or endogenous (73.9–96.4%) compartments were much higher,suggesting that the pea epidermis acts as a selective barrier that filters and enriches microbial communities prior to internal colonization.This study provides comprehensive insights into the mechanisms of host filtering,enrichment and microbial sourcing,which increases our understanding of the assembly rules of the pea-associated fungal microbiome. 展开更多
关键词 β-diversity decomposition fungal community assembly pea source-sink relationships host niche genotype
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GAEP:a comprehensive genome assembly evaluating pipeline 认领 引用 被引量：9
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作者 Yong Zhang Hong-Wei Lu Jue Ruan 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2023年第10期747-754,共8页
With the rapid development of sequencing technologies,especially the maturity of third-generation sequencing technologies,there has been a significant increase in the number and quality of published genome assemblies.... With the rapid development of sequencing technologies,especially the maturity of third-generation sequencing technologies,there has been a significant increase in the number and quality of published genome assemblies.The emergence of these high-quality genomes has raised higher requirements for genome evaluation.Although numerous computational methods have been developed to evaluate assembly quality from various perspectives,the selective use of these evaluation methods can be arbitrary and inconvenient for fairly comparing the assembly quality.To address this issue,we have developed the Genome Assembly Evaluating Pipeline(GAEP),which provides a comprehensive assessment pipeline for evaluating genome quality from multiple perspectives,including continuity,completeness,and correctness.Additionally,GAEP includes new functions for detecting misassemblies and evaluating the assembly redundancy,which performs well in our testing.GAEP is publicly available at http://gffzz188fe103f8f1460asnufu9uo0xc0k69bk.ffgz.tsg.suse.edu.cn/zyoptimistic/GAEP under the GPL3.0 License.With GAEP,users can quickly obtain accurate and reliable evaluation results,facilitating the comparison and selection of high-quality genome assemblies. 展开更多
关键词 Genome assembly Assembly quality Assembly metrics Assembly evaluation pipeline Misassembly detection Misassembly breakpoint Assembly redundancy
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