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Bacillus subtilis enhances maize yield by restricting cadmium translocation and modulating ion homeostasis 认领 引用
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作者 Haseeb Ahmad Jiu Yang Mao +7 位作者 Rayyan Khan Muhammad Ali Shah Kashif Khan Muhammad Asad Naseer Shahid Ali Rida Rashid Rui He Xun Bo Zhou 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第5期311-327,共17页
Cadmium(Cd)pollution in soils severely impacts maize growth.This study investigates the potential application of Bacillus subtilis to soil(BSS)as a bioremediator to mitigate Cd-induced stress during the V8 and VT grow... Cadmium(Cd)pollution in soils severely impacts maize growth.This study investigates the potential application of Bacillus subtilis to soil(BSS)as a bioremediator to mitigate Cd-induced stress during the V8 and VT growth stages of maize.The effects of B.subtilis on maize growth,photosynthesis,antioxidant defense,cellular ultrastructure,osmo-regulatory substances,ion homeostasis,and yield under Cd stress were evaluated.Cd exposure(Cd25 and Cd50 mg/kg)significantly reduced plant height,shoot biomass,leaf area,chlorophyll content,and photosynthetic efficiency by 8%-47%while increasing oxidative stress markers(superoxide anion(O2•-),hydrogen peroxide(H2O2),malondialdehyde(MDA))up to 3-fold.Co-exposure of B.subtilis with Cd stress(Cd25BSS and Cd50BSS)improved these parameters,enhancing plant growth and photosynthetic performance by 7%-40%and reduced stress biomarkers by 8%-38%.Cd25BSS also enhanced superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbate peroxidase(APX),and glutathione reductase(GR)by 12%-34%and upregulated antioxidant genes(ZmSOD1,ZmPOD4,ZmCAT1,ZmAPX1,and ZmGR1)compared to Cd25.Additionally,Cd25BSS increased osmotic regulation through higher soluble sugars and proteins,contributing to cellular stability under stress.Ion homeostasis was improved by increasing essential nutrients from 6%-70%while reducing Cd accumulation by 25%-85%in different maize tissues at both growth stages.Moreover,yield exhibited a strong positive correlation with growth parameters and essential ions(nitrogen,phosphorous,potassium,iron,and zinc),while showing negative correlation with MDA and Cd accumulation.This study highlights B.subtilis as an effective strategy for mitigating Cd stress,improving maize productivity in contaminated soils,and supporting sustainable agricultural practices. 展开更多
关键词 Cadmium Bacillus subtilis Maize Antioxidant defense Ionome Yield
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The potential of metabolites released by Bacillus subtilis CH21 for the effective control of the harmful cyanobacterium Raphidiopsis raciborskii 认领 引用
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作者 Wei Zhang Xu Zeng +4 位作者 Jiayi Dong Yibin Su Erik Jeppesen Liqing Wang Sicheng Yin 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第6期572-580,共9页
Microorganism-based biocontrol strategies for harmful cyanobacterial blooms have gained increasing attention due to their ecological compatibility and species-specific targeting.Despite this,research on algicidal bact... Microorganism-based biocontrol strategies for harmful cyanobacterial blooms have gained increasing attention due to their ecological compatibility and species-specific targeting.Despite this,research on algicidal bacteria effectiveness against the invasive cyanobacterium Raphidiopsis raciborskii remains limited.Research has been conducted on the Bacillus subtilis strain CH21,which was isolated from Chaohu Lake in China.exhibiting potent indirect anticyanobacterial activity against R.raciborskii.Within 48 h of exposure,the cell-free filtrate of strain CH21 achieved 96.2%removal efficiency of R.raciborskii cells.Physiological and morphological analyses revealed severe disruption of the photosynthetic apparatus(Fv/Fm and Yield values reduced to zero within 16 h),oxidative stress(elevation in malondialdehyde content and reactive oxygen species,reduction in superoxide dismutase and catalase activity),and cellular damage,including pseudovacuole collapse,the degradation of polyphosphate granules and cytoplasmic leakage.Metabolomic profiling identified surfactin-class lipopeptides as putative bioactive agents,which demonstrated exceptional stability across a wide thermal(25–121°C)and pH(3–13)range.These findings highlight B.subtilis CH21 as a promising candidate for targeted biocontrol of R.raciborskii blooms,supporting the development of environmentally mitigation technologies to be used in cases where it is difficult to control R.raciborskii by external nutrient loading reduction or if the response to such loading reductions are slow. 展开更多
关键词 Harmful cyanobacterial blooms Algicidal bacteria Raphidiopsis raciborskii Bacillus subtilis
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Impact of dietary supplementation of Bacillus subtilis on the metabolic profiles and microbial ecology of weanling pigs experimentally infected with a pathogenic Escherichia coli 认领 引用
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作者 Kwangwook Kim Sangwoo Park +2 位作者 Cynthia Jinno Peng Ji Yanhong Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期1020-1033,共14页
Background Our previous study demonstrated that dietary supplementation of Bacillus subtilis enhanced growth performance and intestinal integrity in weaned pigs challenged with enterotoxigenic Escherichia coli(ETEC).T... Background Our previous study demonstrated that dietary supplementation of Bacillus subtilis enhanced growth performance and intestinal integrity in weaned pigs challenged with enterotoxigenic Escherichia coli(ETEC).Therefore,this study aimed to explore the impact of Bacillus subtilis on gut health and its role in modulating host–microbe interactions in post-weaning pigs.Results ETEC infection disrupted key metabolic pathways in distal colon,including glutathione,beta-alanine,and pyrimidine metabolism,indicating increased oxidative stress,impaired nucleotide balance,and amino acid catabolic stress.Bacillus subtilis supplementation induced distinct metabolomic and microbiome profiles in colon digesta of weaned pigs challenged with ETEC.Bacillus subtilis-treated pigs under ETEC challenge exhibited significant enrichment in amino acid-and energy-related pathways such as arginine biosynthesis,phenylalanine metabolism,pantothenate and CoA biosynthesis.ETEC infection induced microbial dysbiosis in the distal colon,resulting in decrease(P<0.05)in abundance of Streptococcaceae and Enterobacteriaceae compared to healthy controls.Bacillus subtilis supplementation mitigated the ETEC-induced disruptions by increasing the relative abundance of beneficial bacterial families,including Lachnospiraceae and Bacteroidaceae.Conclusion Supplementation of Bacillus subtilis improves intestinal health and resilience against ETEC challenge by mitigating infection-induced metabolic disruptions and gut dysbiosis in weaned pigs. 展开更多
关键词 Bacillus subtilis Colon microbiota Enterotoxigenic Escherichia coli F18 Metabolomics Weaned pigs
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Growth and Yield Responses of Soledad Chili Pepper(Capsicum annuum L.)to the Application of Chitosan and Bacillus subtilis 认领 引用
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作者 Adolfo Amador Mendoza Rosalba Guadalupe Gomez Raymundo +5 位作者 Ana Rosa Ramírez Seañez Hipolito Hernández Hernández Rogelio Enrrique Palacios Torres Nelda Xanath Martínez Galero Miguelángel García Muñoz Saribel Zilli Gutiérrez 《Phyton-International Journal of Experimental Botany》 SCIE 2026年第1期151-186,共36页
The cultivation of Soledad pepper(Capsicum annuum L.)is essential in Oaxaca and Veracruz,but it faces issues with pests and diseases,which affect yield and cause economic losses.To mitigate these impacts,farmers have ... The cultivation of Soledad pepper(Capsicum annuum L.)is essential in Oaxaca and Veracruz,but it faces issues with pests and diseases,which affect yield and cause economic losses.To mitigate these impacts,farmers have started using biostimulants such as chitosan and plant growth promoting bacteria instead of agrochemicals due to their environmental and health benefits.This study evaluated the effect of Bacillus subtilis and chitosan,both individually and combined,on the growth,yield,and fruit quality of Soledad pepper under greenhouse conditions.Four treatments were applied at different stages of the crop cycle:Q(Chitosan),BS(Bacillus subtilis),Q+BS(Chitosan+Bacillus subtilis),and T(Control).The results showed that the combination of chitosan and Bacillus subtilis significantly improved plant growth,especially in height and stem diameter.The chitosan treatment produced the highest number of fruits and plant weight,while the combination of chitosan and Bacillus subtilis enhanced fruit quality,increasing characteristics such as size,weight,pericarp thickness,and physicochemical parameters,notably in brix degrees,citric acid percentage,and pH,outperforming the individual treatments and the control. 展开更多
关键词 Capsicum annuum L. chitosan Bacillus subtilis fruit quality
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Corrigendum to“Transcriptomic and biochemical analysis of the mechanism of sodium gluconate promoting the degradation of benzo[a]pyrene by Bacillus subtilis MSC4”[Journal of Environmental Sciences,Volume 152,June 2025,Pages 39-53] 认领 引用
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《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第2期789-791,共3页
Rui Chena,b,Tangbing Cui a,b,∗a School of Biology and Biological Engineering,South China University of Technology,Guangzhou 510006,China b Guangdong Key Laboratory of Fermentation and Enzyme Engineering,South China Un... Rui Chena,b,Tangbing Cui a,b,∗a School of Biology and Biological Engineering,South China University of Technology,Guangzhou 510006,China b Guangdong Key Laboratory of Fermentation and Enzyme Engineering,South China University of Technology,Guangzhou 510006,China The authors regret that the published version of this article contained several errors and omissions,which are described and corrected below.1.Figs.3 and 4(figure order and legends).In the published article,Figs.3 and 4 were inadvertently published in reversed order.The figures should be swapped so that the figure content matches its caption.The correct figures and their legends are provided on the following page.2.Title correction.The compound name in the published title was incorrectly typeset as“benzo[a]pyrene”The correct spelling is“benzo[a]pyrene.”3.Text corrections in Section 2.4.Several typographical errors occurred in Section 2.4(“Up-regulation of acetoin,lactate,and kanosamine biosynthesis under sodium gluconate treatment”). 展开更多
关键词 benzo pyrene degradation correction transcriptomic analysis biochemical analysis corrigendum sodium gluconate Bacillus subtilis Msc enzyme engineeringsouth
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Solid-state fermentation of soybean residue by Bacillus subtilis for surfactin production and its application prospects 认领 引用
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作者 ZHAO Feng XU Xiaomeng +2 位作者 LIU Jifang CHEN Mengyang ZHENG Mengyao 《微生物学通报》 CAS CSCD 北大核心 2026年第3期1247-1262,共16页
[Background]Surfactin is a biosurfactant with remarkable surface/interfacial activity.Surfactin production suffers from high costs of carbon sources and severe foaming problem during fermentation.Unreasonable utilizat... [Background]Surfactin is a biosurfactant with remarkable surface/interfacial activity.Surfactin production suffers from high costs of carbon sources and severe foaming problem during fermentation.Unreasonable utilization of soybean residue(okara)can cause resource waste and environmental pollution.[Objective]To achieve sustainable production of surfactin and valueadded conversion of okara,we explored foam-free production of surfactin by Bacillus subtilis using okara as a low-cost substrate and evaluated its application prospects.[Methods]We evaluated and compared the feasibility of B.subtilis utilizing okara to synthesize surfactin through liquid and solid-state fermentation methods.Biosurfactants were extracted from solid-state culture via a weak alkaline water extraction method.The products were identified by HPLC-MS,and the physicochemical properties of the produced surfactin were analyzed.The solid-state medium for fermentation of okara was optimized by the response surface method.The viable count of B.subtilis in solid-state fermentation residue was determined by the plate colony counting method.[Results]The conversion rates of okara to surfactin were 0.6%−0.8%and 1.2%−1.5%in liquid and solid-state fermentation,respectively.Interestingly,solid-state fermentation of okara by B.subtilis achieved both high-yield and foam-free production of surfactin.Five surfactin homologues were produced from okara,mainly including surfactin-C13(34.16%),surfactin-C14(23.95%),and surfactin-C15(35.14%).The produced surfactin,with a critical micelle concentration of 35.0 mg/L,decreased water surface tension to(26.0±0.1)mN/m and emulsified crude oil with emulsifying activity index(EI24)(73.1±3.2)%.It was stable at 4−121℃,pH 5.0−11.0,and NaCl<150 g/L.Okara,NH4Cl,and CaCl2·2H2O were significant components in the solid-state medium.The surfactin yield was increased by 52.1%through solid-state medium optimization.Adding wheat straw further enhanced surfactin production by improving aeration in the solid-state medium.B.subtilis AnPL-1 produced(263.2±7.8)mg surfactin in the optimized solid-state medium containing 14.8 g okara and 1.5 g wheat straw.The conversion rate of okara to surfactin was enhanced to 1.8%.In addition,the residue of solid-state fermentation was expected to be microbial fertilizer since it contained 4.27×1010CFU/g of B.subtilis.[Conclusion]This study established a promising way for foam-free production of surfactin and value-added conversion of okara. 展开更多
关键词 biosurfactants okara solid-state fermentation Bacillus subtilis response surface method
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Transcriptomic and biochemical analysis of the mechanism of sodium gluconate promoting the degradation of benzo [a] pyrene by Bacillus subtilis MSC4 认领 引用 被引量：3
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作者 Rui Chen Tangbing Cui 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2025年第6期39-53,共15页
Benzo[a]pyrene(B[a]P)is a carcinogenic environmental pollutant widely present in the environment and can enter the human body through the food chain.It is therefore essential to treat and remediate the B[a]P-contamina... Benzo[a]pyrene(B[a]P)is a carcinogenic environmental pollutant widely present in the environment and can enter the human body through the food chain.It is therefore essential to treat and remediate the B[a]P-contaminated environment.Microbial remediation of B[a]Pcontaminated environments is considered to be one of the most effective strategies,and the addition of biostimulants is a feasible method to further improve the effectiveness of microbial remediation.In this study,we used Bacillus subtilis MSC4 to screen for the stimulation of sodium gluconate,which promoted B[a]P degradation.Based on biochemical and transcriptomic analyses,Sodium gluconate was found to significantly increase the biomass of MSC4 and the expression of most genes involved in B[a]P degradation.Activities of central carbon metabolism,fatty acidβ-oxidation and oxidative phosphorylation were all promoted.The significant increase in acid-induced oxalate decarboxylase expression indicates a decrease in intracellular pH,which promoted the synthesis of acetoin and lactate.Genes involved in the nitrogen cycle,especially nitrification and denitrification,were significantly up-regulated,contributing to B[a]P degradation.Genes involved in the synthesis of enzyme cofactors,including thiamine,molybdenum cofactors,NAD and heme,were up-regulated,which contributes to increasing enzyme activity in metabolic pathways.Up-regulation of genes in flagella assembly,chemotaxis,and lipopeptide synthesis is beneficial for the dissolution and uptake of B[a]P.Genes related to the sugar transport system were upregulated,which facilitates the transport and absorption of monosaccharides and oligosaccharides by MSC4.This study provides a theoretical basis for the further application of sodium gluconate in the treatment of PAH-contaminated sites. 展开更多
关键词 Transcriptomic Biodegradation Benzo[a]pyrene Bacillus subtilis Sodium gluconate
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Comparative metabolomics reveal histamine biodegradation mechanism by salt stressed Bacillus subtilis JZXJ-7 认领 引用
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作者 Rundong Wang Yijia Deng +3 位作者 Yuhao Zhang Xuepeng Li Ravi Gooneratne Jianrong Li 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第3期925-940,共16页
Bacillus subtilis JZXJ-7 isolated from shrimp paste can significantly degrade histamine under salt stress but the mechanism is unclear.This study aims to evaluate the effect of 170 and 340 mmol/L Na Cl on B.subtilis J... Bacillus subtilis JZXJ-7 isolated from shrimp paste can significantly degrade histamine under salt stress but the mechanism is unclear.This study aims to evaluate the effect of 170 and 340 mmol/L Na Cl on B.subtilis JZXJ-7 growth,histamine degradation,antioxidant enzymes(catalase(CAT),superoxide dismutase(SOD)and glutathione S-transferase(GST))activities and Na+/K+-ATPase activity.Furthermore,comparative metabolomics was used to investigate histamine biodegradation mechanism by B.subtilis JZXJ-7 subjected to salt stress.Both 170 and 340 mmol/L Na Cl promoted B.subtilis JZXJ-7 growth in late stages of reproduction(32-48 h),increased h istamine degradation rate by 64.85%and 79.87%(P<0.05),Na+/K+-ATPase activity to 6.28(P<0.05)and 11.63 U/mg(P<0.01)respectively.N a Cl treatment significantly increased the activities of CAT,GST and SOD(P<0.05),a mino acids and its metabolites(33.39%),benzene and substituted derivatives(12.05%),heterocyclic compounds(10.62%),organic acids and derivatives(9.75%),aldehydes,ketones,esters(5.59%)and nucleotides and its metabolites(4.58%).Metabolite set enrichment analysis revealed Na Cl induced differential metabolic pathways of D-glutamine,D-glutamate,L-arginine,L-proline,histidine and glycerophospholipids,L-lysine degradation,and aminoacyl-t RNA biosynthesis.Exposure to 340 mmol/L Na Cl up-regulated carbohydrate,glutathione and glycerophospholipid metabolism.The new insights into the mechanism of salt stress to promote B.subtilis JZJX-7 growth,energy metabolic pathways and to degrade histamine provide the theoretical basis for application of B.subtilis JZXJ-7 in food fermentation industry. 展开更多
关键词 Bacillus subtilis JZXJ-7 Histamine Salt stress Antioxidant enzymes Metabolomics
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Green synthesis of silver nanoparticles from Bacillus subtilis-mediated feather hydrolysate:antimicrobial,larvicidal against culex pipiens,and anticancer activities 认领 引用
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作者 Mohammed H.Alruhaili Samy Selim +6 位作者 Eslam Adly Mohanned T.Alharbi Bassam M.Al-ahmadi Mutasem S.Almehayawi Soad K.Al Jaouni Salem S.Salem Samah H.Abu-Hussien 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期1865-1890,共26页
This study presents a novel dual-stage bioprocessing approach that transforms poultry feather waste into multifunctional silver nanoparticles(FWH-AgNPs)with enhanced bioactivity.Bacillus subtilis degradation of feathe... This study presents a novel dual-stage bioprocessing approach that transforms poultry feather waste into multifunctional silver nanoparticles(FWH-AgNPs)with enhanced bioactivity.Bacillus subtilis degradation of feather waste produced bioactive hydrolysate(FWH)with dramatically altered chemical composition,generating novel compounds including 9,12,15-octadecatrienoic acid methyl ester(25.66%)and cyclopropaneoctanoic acid methyl ester(23.02%).The FWH effectively synthesized spherical AgNPs(30-69 nm)with strong colloidal stability(−44.5 mV zeta potential)and characteristic surface plasmon resonance(420 nm).FWH-AgNPs demonstrated superior antimicrobial efficacy with 4-eightfold improved minimum inhibitory concentrations against Pseudomonas aeruginosa(125μg/mL),methicillin-resistant Staphylococcus aureus(250μg/mL),Aspergillus brasiliensis(275μg/mL),and Candida albicans(125μg/mL).Comparable enhancements were also observed for Serratia marcescens(300μg/mL)and Bacillus cereus(325μg/mL),further confirming the broad-spectrum antimicrobial potential of FWH-AgNPs.Anticancer evaluation revealed selective cytotoxicity toward MCF-7 breast cancer cells(IC50:294.7μg/L)with favorable selectivity index(2.68)over normal fibroblasts.Optimized FWH-AgNPs achieved 87.38%larvicidal mortality against Culex pipiens,validated through Box-Behnken methodology.Mechanistic studies revealed systematic disruption of larval metabolism,including protein depletion,carbohydrate exhaustion,and acetylcholinesterase inhibition,coupled with severe midgut epithelial damage.Molecular docking identifiedα1-sitosterol as the primary bioactive compound with strong binding affinities to antimicrobial targets(−7.1 to−7.4 kcal/mol)and cancer receptors(−7.0 to−9.5 kcal/mol).This integrated approach successfully addresses environmental waste management while generating high-value nanomaterials for biomedical and vector control applications,establishing a new paradigm for circular bioeconomy applications. 展开更多
关键词 Feather waste bioprocessing Bacillus subtilis Silver nanoparticles Antimicrobial activity Anticancer therapy Larvicidal activity
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Bacillus subtilis ge28,a potential biocontrol agent for controlling rust rot in Panax ginseng caused by Cylindrocarpon destructans 认领 引用
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作者 Yuan Gao Junchi Wang +4 位作者 Shan Zhong Rong Wang Jianhe Wei Wanlong Ding Yong Li 《Medicinal Plant Biology》 2025年第1期337-346,共10页
Panax ginseng is a traditional herbal medicine used worldwide.Rust rot caused by Cylindrocarpon destructans infects the root of P.ginseng and usually results in severe yield losses and quality deterioration.Quality co... Panax ginseng is a traditional herbal medicine used worldwide.Rust rot caused by Cylindrocarpon destructans infects the root of P.ginseng and usually results in severe yield losses and quality deterioration.Quality control of P.ginseng is crucial to ensure its medicinal value.However,the increasing application of chemical fungicides for controlling severe epidemics of diseases including rust rot in ginseng-producing regions every year has led to significant environmental pollution,fungicide residues in harvested ginseng,and an increased risk of phytopathogen resistance.In this study,an endophytic Bacillus subtillis strain,temporarily named ge28,which has shown strong antagonistic activity to C.destructans,was isolated from healthy roots of P.ginseng.In vitro and pot experiments showed fermentation and the supernatant of ge28 exhibited significant biocontrol efficacy on rust rot.The whole genome of ge28 was 4,065,429 bp,containing 4,162 functional genes.Furthermore,three clusters of non-ribosomal peptide synthetase genes conferring the synthesis of lipopeptides,a group of secondary metabolites exhibiting antagonistic properties,including surfactin,fengycin,and bacillibactin,were identified through genome analysis.Surfactin and fengycin were confirmed to be present in the supernatant of ge28 by UPLC-ESI-QTOF-MS analysis.In conclusion,this study demonstrated that the ge28 strain possesses substantial biocontrol potential against the rust rot of P.ginseng,and its antagonistic mechanisms were preliminarily clarified.These results should accelerate the utilization of ge28 to enhance the yield and quality of P.ginseng through reduced fungicide application. 展开更多
关键词 Ge rust rot Bacillus subtilis Biocontrol cylindrocarpon destructans panax ginseng chemical fungicides traditional herbal medicine
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Enhanced antioxidant activity of brown seaweed Laminaria japonica by fermentation using isolated Bacillus subtilis 认领 引用 被引量：1
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作者 Yueh-Hao Ronny Hung Ya-Han Chang +2 位作者 Hsuan-Ju Lin Li-Ho Chiang Hong-Ting Victor Lin 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期1112-1125,共14页
Bioactive compounds from the brown seaweed Laminaria japonica have received considerable attention from the food industries to explore their biological activities.However,effectively producing bioactive compounds from... Bioactive compounds from the brown seaweed Laminaria japonica have received considerable attention from the food industries to explore their biological activities.However,effectively producing bioactive compounds from seaweed requires further investigation because of the complex composition of seaweed cell walls.In the present study,Bacillus subtilis strains were isolated and screened for the production of extracellular hydrolytic enzymes for the fermentation of L.japonica to enhance its antioxidant activity.Three B.subtilis strains were successfully isolated from commercial natto products,and their hydrolytic abilities were evaluated using fibrinogen,alginate,and cellulose plates.Strain B2 exhibited great application potential in degrading seaweed cell wall polysaccharides compared with other strains.Subsequently,strain B2 was utilized for seaweed fermentation,and chemical composition alterations(including phenolic compounds,total sugar,and reducing sugar)of the fermented broth were analyzed.A high sulfate content was detected in the polysaccharide fraction of the fermented seaweed.Finally,the antioxidant activities(ferrous iron-chelating ability,reducing power,ABTS radical-scavenging ability,and DPPH radical-scavenging ability)of the supernatant,polysaccharide,and polyphenol fractions from the fermented seaweed were assessed and compared with those of the nonfermented groups.Results indicated that fermentation with B.subtilis could enhance the antioxidant activity in supernatant,polysaccharide,and polyphenol fractions.Fermentation acted positively on the bioconversion of L.japonica extract,thereby improving the antioxidant properties.Overall,this study provides valuable insights into enhancing the antioxidant activities of L.japonica through fermentation. 展开更多
关键词 Laminaria japonica Bacillus subtilis Fermentation Antioxidant Polysaccharide Phenolic compounds
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Enhancement of Menaquinone-7 production in Bacillus subtilis by optimizing the medium components through response surface methodology 认领 引用 被引量：1
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作者 Rui Zhang Han Wang +1 位作者 Li Wang Zhiming Zheng 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期1466-1483,共18页
Menaquinone-7,a form of vitamin K2,plays a critical role in the treatment of hemorrhagic diseases caused by vitamin K deficiency and in the prevention of bone fractures.Microbial fermentation has emerged as a promi... Menaquinone-7,a form of vitamin K2,plays a critical role in the treatment of hemorrhagic diseases caused by vitamin K deficiency and in the prevention of bone fractures.Microbial fermentation has emerged as a promising method for MK-7 production due to its high product optical purity and significant physiological activity.However,the current production efficiency of MK-7 remains insufficient to meet industrial demands.In this study,we employed a combination of single-factor experiments,Plackett-Burman design,steepest ascent experiments,and Box-Behnken design to optimize the fermentation medium for MK-7 production by Bacillus subtilis BS-ΔackA.Through regression analysis and consideration of practical production constraints,the optimal fermentation medium parameters were determined as follows:20 g/L sucrose,20.7 g/L glycerol,47.3 g/L soy peptone,4 g/L yeast extract,and 1.9 g/L KH2PO4,0.1 g/L MgSO4·7 H2O.Under these optimized conditions,the MK-7 yield reached 154.6±1.32 mg/L.The experimental results demonstrated excellent stability and reliability,aligning closely with the model predictions.This study significantly enhances MK-7 production at the shake-flask fermentation level,providing valuable insights for large-scale industrial applications. 展开更多
关键词 Bacillus subtilis MK-7 Medium optimization Response surface methodology
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Corrosion behavior of aluminum alloys by Bacillus subtilis in artificial seawater 认领 引用
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作者 Ya-hui Hu Fu-yan Kang +5 位作者 Qian-yun Tan Shu-ying Pu Li-xiang Yang Xiao-ya Ding Wei-hua Li Fa-qian Liu 《Journal of Iron and Steel Research International》 SCIE EI CAS CSCD 2025年第8期2596-2608,共13页
The impact of Bacillus subtilis(B.subtilis)on the corrosion behavior(microbiologically influenced corrosion)of two aluminum alloys—7075 aluminum alloy(AA7075)and 2024 aluminum alloy(AA2024)—is investigated in a high... The impact of Bacillus subtilis(B.subtilis)on the corrosion behavior(microbiologically influenced corrosion)of two aluminum alloys—7075 aluminum alloy(AA7075)and 2024 aluminum alloy(AA2024)—is investigated in a high-salinity environment.The corrosion mechanism is explored by observing the bacterial growth process and the corresponding measurements.The assessment involves the analysis of surface morphology,corrosion mass loss,and electrochemical tests.Surface morphology reveals the development of B.subtilis and biofilm formation on the surface of the aluminum alloys.Both mass loss and electrochemical tests illustrate the corrosion inhibition effect of B.subtilis on aluminum alloys in a high-salt environment.AA7075 alloy was more affected by the biofilm and had a more pronounced corrosion inhibition effect.After 10 d of testing,in B.subtilis group,AA7075 and AA2024 bode values were elevated by 0.32×104 and 0.24×104Ω,respectively.The open circuit potential of AA2024 decreased by−0.107 A/cm2 under the influence of bacteria,and the dynamic potentiodynamic polarization curve shifted significantly to the left. 展开更多
关键词 Aluminum alloy Bacillus subtilis Electrochemistry Microbiological corrosion Biofilm
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Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase 认领 引用 被引量：17
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作者 刘燕 陶晶 +3 位作者 阎豫君 李彬 李晖 李春 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2011年第1期128-134,共7页
The seed germination and tomato seedling tests showed that Bacillus subtilis SL-13 could promote the sprouting and seedling growth of tomato.The fresh and dry weight of tomato seedlings increased 42.86%and 18.75%,resp... The seed germination and tomato seedling tests showed that Bacillus subtilis SL-13 could promote the sprouting and seedling growth of tomato.The fresh and dry weight of tomato seedlings increased 42.86%and 18.75%,respectively.The control efficacies of the SL-13 to tomato Rhizoctonia rot were 20.65%and 35.23%in the greenhouse and field,respectively.The growth of the plant-pathogenic fungus Rhizoctonia solani was considerably inhibited in the presence of the strain SL-13 culture supernatant.The main antifungal protein was detected to be chitinase through vitro assay.The chitinase was purified with DEAE-Sepharose fast flow ion exchange column chromatography and Sephadex G-75 gel filtration for further characterization.The optimal pH and temperature for the chitinase activity were 7.0 and 50°C,respectively.It was demonstrated that the enzyme was stable at pH 5-9 and 40-60°C.70%of the enzyme activity was retained when incubated at 121°C and 0.11 MPa for 20 min,and the enzyme was not sensitive to protease K and ultraviolet radiation.Thus it is suitable for effective biological control in relatively unstable environment. 展开更多
关键词 Bacillus subtilis growth-promoting diseases control antifungal chitinase purification Rhizoctonia solani
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Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29 认领 引用 被引量：24
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作者 Jing LI Qian YANG +3 位作者 Li-hua ZHAO Shu-mei ZHANG Yu-xia WANG Xiao-yu ZHAO 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS 2009年第4期264-272,共9页
An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl （DEAE）-52 cellulose and gel filtration chromatogr... An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl （DEAE）-52 cellulose and gel filtration chromatography on Bio-Gel P-100. The protein was absorbed on DEAE-cellulose and Bio-Gel P-100. The purified antifungal fraction was designated as B29I, with a molecular mass of 42.3 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）, pl value 5.69 by isoelectric focusing （IEF）-PAGE, and 97.81% purity by high performance liquid chromatography （HPLC）. B291 exhibited inhibitory activity on mycelial growth in Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme, and Sclerotinia sclerotiorum. The 50% inhibitory concentrations （IC50） of its antifungal activity toward Fusarium oxysporum and Rhizoctonia solani were 45 and 112 μmol/L, respectively. B29I also demonstrated an inhibitory effect on conidial spore germination of Fusariurn oxysporum and suppression of germ-tube elongation, and induced distortion, tumescence, and rupture of a portion of the germinated spores. 展开更多
关键词 Bacillus subtilis Antifungal protein Purification
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干旱胁迫下枯草芽孢杆菌(Bacillus subtilis)对柠条(Caragana korshinskii)和沙冬青(Ammopiptanthus mongolicus)种子萌发及幼苗生长的影响 认领 引用 被引量：16
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作者 王雨 刘振婷 +5 位作者 高广磊 杜凤梅 张英 丁国栋 任悦 曹红雨 《中国沙漠》 CSCD 北大核心 2022年第5期73-81,共9页
土壤微生物具有丰富的功能多样性和极强的环境适应性,微生物固沙成为防沙治沙技术研发的新兴领域。枯草芽孢杆菌(Bacillus subtilis)是常用的微生物固沙材料,但已有研究多聚焦于其防风固沙效果,较少注意其对沙生植物定植和生长生理的影... 土壤微生物具有丰富的功能多样性和极强的环境适应性,微生物固沙成为防沙治沙技术研发的新兴领域。枯草芽孢杆菌(Bacillus subtilis)是常用的微生物固沙材料,但已有研究多聚焦于其防风固沙效果,较少注意其对沙生植物定植和生长生理的影响。为揭示枯草芽孢杆菌对沙生植物种子萌发和幼苗生长生理特征的影响,以柠条(Caragana korshinskii)和沙冬青(Ammopiptanthus mongolicus)为研究对象,开展室内盆栽试验,对比分析不同干旱梯度和菌剂浓度下种子萌发、幼苗生长和生理生化特征。结果表明:(1)重度干旱显著抑制柠条种子萌发和幼苗生长(P0.01),但对二者叶绿素、丙二醛和脯氨酸含量存在极显著影响(P<0.01)。枯草芽孢杆菌可以通过影响两种沙生植物生理生化活动间接影响种子萌发和幼苗生长,提高植物抗逆性。 展开更多
关键词 枯草芽孢杆菌(Bacillus subtilis) 沙冬青(Ammopiptanthus mongolicus) 柠条(Caragana korshinskii) 干旱胁迫 种子萌发 幼苗生长
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Removal of ammonium-N from ammonium-rich sewage using an immobilized Bacillus subtilis AYC bioreactor system 认领 引用 被引量：12
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作者 Jingjing Xiao Changxiong Zhu +2 位作者 Dongyuan Sun Ping Guo Yunlong Tian 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2011年第8期1279-1285,共7页
A self-design bioreactor system employing a fixed bed operation process with immobilized Bacillus subtilis AYC beads Ibr NH4^-N removal from slightly polluted water was proposed.Polyvinyl alcohol and Na-alginate were ... A self-design bioreactor system employing a fixed bed operation process with immobilized Bacillus subtilis AYC beads Ibr NH4^-N removal from slightly polluted water was proposed.Polyvinyl alcohol and Na-alginate were used as a gel matrix to entrap Bacillus subtilis AYC to form the immobilized beads.The NH4+-N removal process was studied in a intermittent operation mode to examine the start-up and steady state behaviors of the immobilized AYC in the reactor.The results indicated that the reactor was in the start-up state during the first week.NH4+-N began to be steadily removal since the second week,and the nitrogen removal rate was between 84.61%and 96.19%when the hydraulic retention time(HRT)was 30 rain.To apply Bacillus subtilis AYC to develop a practical nitrogen removal system and further understand its nitrogen removal ability,the bioreactor was continuously operated under different experimental perameters.The results showed that under the optimum conditions of an HRT of 20 min and DO of 3.77-5.80 mg/L,the NH4+-N removal rate reached 99.55%.The NH4+-N removal rate increased as the C/N ratio increased.However,a high C]N may cause a high residual carbon level in the effluent,therefore,the most suitable C/N ratio was 10.In addition,the results showed that the bioreactor system could remove many types of nitrogen such as NH4+-N,NO3--N and organic-N,and had a good performance for inorganic nitrogen removal from sewage. 展开更多
关键词 immobilized Bacillus subtilis AYC beads biologic nitrogen removal bioreactor effect
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The PhoR/PhoP two-component system regulates fengycin production in Bacillus subtilis NCD-2 under low-phosphate conditions 认领 引用 被引量：8
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作者 GUO Qing-gang DONG Li-hong +5 位作者 WANG Pei-pei LI She-zeng ZHAO Wei-song LU Xiu-yun ZHANG Xiao-yun MA Ping 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第1期149-157,共9页
Bacillus subtilis strain NCD-2 is an excellent biocontrol agent for plant soil-borne diseases, and the lipopeptide fengycin is one of the active antifungal compounds in strain NCD-2. The regulator PhoP and its sensor ... Bacillus subtilis strain NCD-2 is an excellent biocontrol agent for plant soil-borne diseases, and the lipopeptide fengycin is one of the active antifungal compounds in strain NCD-2. The regulator PhoP and its sensor kinase PhoR compose a two-component system in B. subtilis. In this study, the phoR- and phoP-knockout mutants were constructed by in-frame deletion and the role of PhoR/PhoP on the production of fengycin was determined. Inactivation of phoR or phoP in B. subtilis decreased its inhibition ability against Botrytis cinerea growth in vitro compared to the strain NCD-2 wild type. The lipopeptides were extracted from strain NCD-2 wild type and its mutant strains by hydrochloric acid precipitate, and the lipopeptides from phoR-null mutant orphoP-null mutant almost lost the inhibition ability against B. cinerea growth compared to the lipopeptides from strain NCD-2 wild type. Fast protein liquid chromatography （FPLC） analysis of the lipopeptides showed that inactivation of phoR or phoP genes reduced the production of fengycin by strain NCD-2. The fengycin production abilities were compared for bacteria under low-phosphate medium （LPM） and high-phosphate medium （HPM）, respectively. Results indicated that the regulation of fengycin production by the PhoR/PhoP two-component system occurred in LPM but not in HPM. Reverse transcriptionaI-PCR confirmed that the fengycin synthetase gene fenC was positively regulated by phoP when cultured in LPM. All of these characteristics could be partially restored by complementation of intact phoR or phoP gene in the mutant. These data indicated that the PhoR/PhoP two-component system greatly regulated fengycin production and antifungal ability in B. subtilis NCD-2 mainly under low-phosphate conditions. 展开更多
关键词 Bacillus subtilis two-component system Iipopeptides transcriptional factor PhoR/PhoP
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A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis 认领 引用 被引量：13
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作者 Jing Ye Yunjie Li +5 位作者 Yuqing Bai Ting Zhang Wei Jiang Ting Shi Zijian Wu Yi-Heng P.Job Zhang 《Bioresources and Bioprocessing》 SCIE 2022年第1期997-1008,共12页
To mimic the Escherichia coliT7 protein expression system,we developed a facile T7 promoter-based protein expression system in an industrial microorganism Bacillus subtilis.This system has two parts:a new B.subtilisst... To mimic the Escherichia coliT7 protein expression system,we developed a facile T7 promoter-based protein expression system in an industrial microorganism Bacillus subtilis.This system has two parts:a new B.subtilisstrain SCK22 and a plasmid pHT7.To construct strain SCK22,the T7 RNA polymerase gene was inserted into the chromosome,and several genes,such as two major protease genes,a spore generation-related gene,and a fermentation foam generation-related gene,were knocked out to facilitate good expression in high-density cell fermentation.The gene of a target protein can be subcloned into plasmid pHT7,where the gene of the target protein was under tight control of the T7 promoter with a ribosome binding site(RBS)sequence of B.subtilis(i.e.,AAG GAG G).A few recombinant proteins(i.e.,green fluorescent protein,α-glucan phosphorylase,inositol monophosphatase,phosphoglucomutase,and 4-α-glucanotransferase)were expressed with approximately 25-40%expression levels relative to the cellular total proteins estimated by SDS-PAGE by using B.subtilisSCK22/pHT7-derived plasmid.A fed-batch high-cell density fermentation was conducted in a 5-L fermenter,producing up to 4.78 g/L inositol monophosphatase.This expression system has a few advantageous features,such as wide applicability for recombinant proteins,high protein expression level,easy genetic operation,high transformation efficiency,good genetic stability,and suitability for high-cell density fermentation. 展开更多
关键词 Bacillus subtilis T7 expression system Recombinant protein expression High cell-density fermentation
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Effect of dietary supplementation of Bacillus subtilis B10 on biochemical and molecular parameters in the serum and liver of high-fat diet-induced obese mice 认领 引用 被引量：7
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作者 Kai LEI Ya-li LI +4 位作者 Yang WANG Jing WEN Hong-zhao WU Dong-you YU Wei-fen LI 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2015年第6期487-495,共9页
While a high-fat diet（HFD） is assumed to be related to fat-mediated oxidative stress decreasing antioxidant enzyme activity, probiotics are believed to have positive effects on the regulation of HFD-induced obesity ... While a high-fat diet（HFD） is assumed to be related to fat-mediated oxidative stress decreasing antioxidant enzyme activity, probiotics are believed to have positive effects on the regulation of HFD-induced obesity as well as lipid metabolism, energy homeostasis, and anti-oxidation. Because Bacillus subtilis B10 has beneficial effects on the abnormal lipid metabolism and the oxidative stress in HFD-induced obese mice, ICR mice were randomly assigned into an HFD group and the HFD was supplemented with 0.1%（w/w） Bacillus subtilis B10（HFD＋B10 group）. Thereafter, 30-d treatments were run, and then hepatic lipid level and antioxidant status were measured. The expression of genes related to lipid metabolism and oxidative stress in the liver was determined by reverse-transcription quantitative polymerase chain reaction（RT-q PCR）. We found that HFD-induced obese mice treated with B10 showed a decrease in weight gain, serum glucose activity as well as hepatic triglyceride（TG）, glutamic oxaloacetic transaminase（GOT）, and glutamic pyruvic transaminase（GPT） activities. In addition, the gene expressions of antioxidant genes, glutathione reductase（GR）, xanthine oxidase（XO）, heat-shock protein 90（Hsp90）, and lipid synthesis gene 3β-hydroxysteroid-？24 reductase（DHCR24） in the HFD＋B10 group were down-regulated, suggesting alleviation of oxidative stress, while the lipolysis gene 3-hydroxy-3-methylglutaryl-Co A synthase 2（HMGCS2）, energy metabolism gene peroxisome proliferator-activated receptor α（PPARα） and the gene encoding tumor-suppressor protein p53 were up-regulated. The regulatory and positive effect of dietary supplementation of probiotic B10 suggests that it has a beneficial effect on the homeostasis of the lipid metabolism and on alleviating oxidative stress in HFD-induced obese mice. 展开更多
关键词 Bacillus subtilis High-fat diet Oxidative stress Lipid metabolism
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