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Lactobacillus acidophilus attenuates polyethylene glycol-induced susceptibility to Citrobacter rodentium infection via microbiota modulation 认领 引用 被引量：1
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作者 Guan-Jun Kou Jing Shen +2 位作者 Li-Xiang Li Xiu-Li Zuo Yan-Qing Li 《World Journal of Gastroenterology》 SCIE CAS 2026年第11期90-101,共12页
BACKGROUND Polyethylene glycol(PEG),a globally prevalent laxative,is extensively applied for bowel preparation prior to colonoscopy or abdominal surgery due to its exceptional safety profile.Nevertheless,emerging evid... BACKGROUND Polyethylene glycol(PEG),a globally prevalent laxative,is extensively applied for bowel preparation prior to colonoscopy or abdominal surgery due to its exceptional safety profile.Nevertheless,emerging evidence suggests that PEG administration might induce significant alterations in colonic microbiota composition,with dysbiotic effects persisting for over two weeks.The potential implications of such microbiotic perturbations concerning opportunistic pathogen colonization and invasion remain elusive.AIM To investigate whether PEG increases the risk of intestinal infection,and whether Lactobacillus acidophilus(LAC)can mitigate the adverse effects and thereby reduce the risk of subsequent enteric infections.METHODS To investigate this,we employed a murine model infected with Citrobacter rodentium(CR),a surrogate for enteropathogenic Escherichia coli and enterohemorrhagic Escherichia coli,following PEG-induced bowel preparation.RESULTS The severity of infectious enteritis was assessed,and the prophylactic efficacy of LAC supplementation was evaluated.Our findings indicate that PEG administration significantly elevated CR load,enhanced virulence gene expression,and exacerbated intestinal inflammation post-infection,with the infection window extending approximately 14 days.Through fecal metagenomic analysis and co-housing experiments,we demonstrated that PEGassociated enteritis is critically mediated by gut microbiota dysbiosis.Furthermore,supplementation with LAC was shown to mitigate susceptibility to CR infection.CONCLUSION Our results suggest that PEG administration microbiota-dependently increases intestinal infection susceptibility,highlighting the therapeutic potential of LAC in restoring microbial homeostasis. 展开更多
关键词 Polyethylene glycol Citrobacter rodentium Enteropathogenic Escherichia coli Enterohemorrhagic Escherichia coli Gut microbiota Lactobacillus acidophilus
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Citrobacter freundii作用下球状碳酸盐矿物的形态演化过程研究 认领 引用 被引量：11
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作者 马恒 李福春 +1 位作者 苏宁 李学林 《高校地质学报》 CAS 北大核心 2009年第3期429-436,共8页
不少微生物都具有形成球状碳酸盐矿物的能力。对形成过程的动态跟踪研究是了解球状碳酸盐矿物形成机理的关键。利用光学显微镜和电子显微镜对Citrobacter freundii作用下球状碳酸盐矿物的形成过程进行了跟踪研究。结果表明,纳米晶体在... 不少微生物都具有形成球状碳酸盐矿物的能力。对形成过程的动态跟踪研究是了解球状碳酸盐矿物形成机理的关键。利用光学显微镜和电子显微镜对Citrobacter freundii作用下球状碳酸盐矿物的形成过程进行了跟踪研究。结果表明,纳米晶体在有机物质的包裹下逐渐聚集,最终生长为具有辐射状内部结构和菱形表面纹理的球状构造。在球状构造形成之前,矿物集合体经历了花形、哑铃形、花菜形、纺锤形以及类似于十字形的多种过渡形态。对最初、过渡和最终形态进行总结,有助于理解微生物作用下的球状碳酸盐矿物形成机理,为识别微生物活动在沉积环境中遗留的痕迹提供更多有用的信息。以往的研究极少涉及Citrobacter freundii诱导碳酸盐矿物的形成。研究结果将丰富微生物促进球状碳酸盐矿化的研究内容。 展开更多
关键词 Citrobacter freundii 生物矿化 碳酸盐矿物 球状 哑铃状
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Citrobacter sp.LW-3对偶氮染料甲基橙的降解脱色特性研究 认领 引用 被引量：8
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作者 王莉 李琛琛 蒋海兵 《环境科学学报》 CAS CSCD 北大核心 2014年第9期2213-2218,共6页
从长期受染料污染的土壤中分离出一株能对甲基橙高效降解脱色的柠檬酸盐杆菌Citrobacter sp.LW-3.菌株LW-3在添加了0.5%(g/100 mL)葡萄糖的MSM-1培养基中,16 h使100 mg·L-1甲基橙降解掉90.56%.该菌株对甲基橙降解脱色可在完全好氧... 从长期受染料污染的土壤中分离出一株能对甲基橙高效降解脱色的柠檬酸盐杆菌Citrobacter sp.LW-3.菌株LW-3在添加了0.5%(g/100 mL)葡萄糖的MSM-1培养基中,16 h使100 mg·L-1甲基橙降解掉90.56%.该菌株对甲基橙降解脱色可在完全好氧条件下,脱色与木质素过氧化物酶、NADH-DCIP原酶、核黄素还原酶有关.菌株LW-3降解甲基橙的适宜温度较广,20~40℃24 h均能使超过80%的甲基橙降解脱色,适宜pH范围为6.0~8.0.通气量对菌株LW-3影响较小,24 h各装液量组(25~200 mL)甲基橙均能降解近90%.同时,菌株LW-3具有较广的染料降解谱,3 d内可使20 mg·L-1另3种偶氮染料、两种三苯甲烷类染料和一种蒽醌类染料很好脱色.菌株LW-3在染料废水的生物处理方面有较强的实际应用价值. 展开更多
关键词 甲基橙 Citrobacter sp.LW-3 降解脱色特性 脱色酶 染料降解谱
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微生物燃料电池中一株产电菌Citrobacter freundii的分离及特性研究 认领 引用 被引量：4
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作者 李颖 孙永明 +3 位作者 孔晓英 李连华 李东 袁振宏 《太阳能学报》 EI CAS CSCD 北大核心 2012年第11期1968-1972,共5页
通过形态、生理生化特性及系统进化分析表明,从微生物燃料电池中分离出的产电菌株LY-3与弗氏柠檬酸杆菌(Citrobacter freundii)最相近,将其命名为Citrobacter freundii LY-3。循环伏安扫描显示,该菌株具有电化学活性,在微生物燃料电池中... 通过形态、生理生化特性及系统进化分析表明,从微生物燃料电池中分离出的产电菌株LY-3与弗氏柠檬酸杆菌(Citrobacter freundii)最相近,将其命名为Citrobacter freundii LY-3。循环伏安扫描显示,该菌株具有电化学活性,在微生物燃料电池中,以牛肉膏为燃料产生的最大电压为0.43V,最大功率密度为98.2mW/m2。 展开更多
关键词 微生物燃料电池 Citrobacter freundii 系统进化树 电化学活性
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Citrobacter sp.EBS8对施氏矿物和黄钾铁矾的还原溶解及相转化行为的影响 认领 引用 被引量：1
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作者 姜梦戈 赖伟斌 +3 位作者 曾宇飞 卢桂宁 党志 郭楚玲 《环境科学学报》 CAS CSCD 北大核心 2020年第11期3858-3867,共10页
铁硫酸盐次生矿物广泛存在于酸性矿山废水(AMD)污染流域沉积物中,是多种有毒有害重金属的沉淀库.硫酸盐还原菌对矿物的还原作用可破坏矿物的稳定性从而引起共沉淀重金属的释放.在前期研究中发现柠檬酸杆菌Citrobacter sp.存在于AMD流域... 铁硫酸盐次生矿物广泛存在于酸性矿山废水(AMD)污染流域沉积物中,是多种有毒有害重金属的沉淀库.硫酸盐还原菌对矿物的还原作用可破坏矿物的稳定性从而引起共沉淀重金属的释放.在前期研究中发现柠檬酸杆菌Citrobacter sp.存在于AMD流域沉积物中,且在还原条件下具有较强的硫酸盐还原能力.因此,探究其对铁硫次生矿物的还原溶解及矿物晶相转化行为的影响尤为重要.本研究选取矿区沉积物中典型的铁硫次生矿物施氏矿物和黄钾铁矾为研究对象,在实验室条件下考察了从AMD污染沉积物中筛选到的菌株Citrobacter sp. EBS8对矿物的还原溶解及相转化的影响.结果表明,在缺氧、中性pH及电子供体足量时,在EBS8的作用下施氏矿物和黄钾铁矾均出现了明显的还原溶解现象.菌株EBS8可利用乳酸盐作为电子供体还原SO42-和Fe(Ⅲ).SEM结果显示,施氏矿物在反应初期出现表面毛刺不平现象,黄钾铁矾发生由外向内的逐层溶解,矿物转化过程中都出现空壳形貌.XRD检测到施氏矿物组的主要转化产物为菱铁矿和蓝铁矿,黄钾铁矾体系中主要是蓝铁矿和马基诺矿.这些结果有助于了解AMD污染沉积物中铁硫酸盐矿物的转化行为及为AMD污染治理提供理论支撑. 展开更多
关键词 铁硫酸盐次生矿物 Citrobacter sp. 还原 溶解 相转化
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Citrobacter farmeri降解间甲酚的特性分析 认领 引用 被引量：2
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作者 彭丽花 董佳 《工业用水与废水》 CAS 2011年第1期28-31,53,共4页
从某焦化厂废水好氧生物处理的污泥中分离出一株能以间甲酚为唯一碳源、能源生长代谢的菌株Citro-bacter farmeri。考察了间甲酚在不同初始浓度下Citrobacter farmeri细菌生长动力学,4种等浓度酚类物质的降解先后顺序,分别添加共基质葡... 从某焦化厂废水好氧生物处理的污泥中分离出一株能以间甲酚为唯一碳源、能源生长代谢的菌株Citro-bacter farmeri。考察了间甲酚在不同初始浓度下Citrobacter farmeri细菌生长动力学,4种等浓度酚类物质的降解先后顺序,分别添加共基质葡萄糖和蛋白胨对低浓度和高浓度间甲酚降解的影响,以及在添加共基质葡萄糖时Citrobacter farmeri降解高浓度间甲酚过程中产生的酶活。结果表明,当间甲酚初始质量浓度低于375 mg/L时,Citrobacter farmeri菌株生长符合一级动力学方程;Citrobacter farmeri降解4种酚类物质的降解顺序依次为苯酚>邻甲酚>间甲酚>萘酚;外加葡萄糖或蛋白胨对质量浓度低于375 mg/L的间甲酚的降解没有影响,但对高于375mg/L的间甲酚降解有明显的促进作用,在最佳葡萄糖添加量为800 mg/L或最佳蛋白胨添加量为640 mg/L时,缩短降解时间分别为30 h和29 h;对酶活的测定发现儿茶酚1,2-双加氧酶和儿茶酚2,3-双加氧酶有明显增大,初步判断Citrobacter farmeri以邻位和间位裂解的途径对间甲酚进行降解。 展开更多
关键词 Citrobacter farmeri 生物降解 间甲酚 代谢途径 邻苯二酚双加氧酶
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Establishment and Comparison of Pulsed-field Gel Electrophoresis,Multiple-locus Variable Number Tandem Repeat Analysis and Automated Ribotyping Methods for Subtyping of Citrobacter Strains 认领 引用 被引量：1
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作者 ZHANG Xiao Ai BAI Xue Mei +2 位作者 YE Chang Yun REN Zhi Hong XU Jian Guo 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2012年第6期653-662,共10页
Objective To establish and compare the pulsed-field gel electrophoresis （PFGE）, multiple-locus variable number tandem repeat analysis （MLVA） and automated ribotyping for subtyping of Citrobacter strains. Methods P... Objective To establish and compare the pulsed-field gel electrophoresis （PFGE）, multiple-locus variable number tandem repeat analysis （MLVA） and automated ribotyping for subtyping of Citrobacter strains. Methods PFGE protocol was optimized in terms of plug preparation procedure, restriction enzymes and configuration of electrophoretic parameters. MLVA method was evaluated by finding variable number tandem repeats in two genomes of Citrobacter strains. The ribotyping was performed by using the automated RiboPrinter system. Results We optimized the plug preparation procedure, focused on the cell suspension concentration （turbidity of 2.5 to 3.5）, SDS addition （no SDS needed） and lysis time （1 h）, and selected the appropriate restriction enzyme （Xbal） and the electrophoretic parameters （1.0 s-20.0 s for 19 h） of PFGE. There was nearly no discriminatory power of MLVA between Citrobacter strains. For 51 Citrobacter strains, automated ribotyping gave a D-value of 0.9945, while PFGE gave a D-value of 0.9969. Both PFGE and automated ribotyping clustered strains from the same sources （with the same species from the same place at the same time identified as the same source） and divided strains from different sources （from different years, places and hosts） into different subtypes. Conclusion PFGE protocol established in this paper and automated ribotyping are suitable for application in Citrobacter subtyping. 展开更多
关键词 Citrobacter Pulsed-field gel electrophoresis Multiple-locus variable number tandem repeatanalysis Ribotyping Molecular subtyping
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Effect of surfactant on phenanthrene metabolic kinetics by Citrobacter sp. SA01 认领 引用 被引量：5
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作者 Feng Li Lizhong Zhu Dong Zhang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2014年第11期2298-2306,共9页
To attain a better understanding of the effects of surfactants on the metabolic kinetics of hydrophobic organic compounds, the biodegradation of phenanthrene by Citrobacter sp. SA01 was investigated in a batch experim... To attain a better understanding of the effects of surfactants on the metabolic kinetics of hydrophobic organic compounds, the biodegradation of phenanthrene by Citrobacter sp. SA01 was investigated in a batch experiment containing Tween 80, sodium dodecyl benzene sulfonate and liquid mineral salt medium. The Monod model was modified to effectively describe the partition, phenanthrene biodegradation and biopolymer production. The results showed that Tween 80 and sodium dodecyl benzene sulfonate （each at 50 rag/L） enhanced phenanthrene metabolism and poly-β-hydroxybutyrate production as indicated by the increasing amounts of intermediates Coy 17.2% to 47.9%）, and percentages of poly-β- hydroxybutyrate （by 107.3% and 33.1%） within the cell dry weight when compared to their absence. The modified Monod model was capable of predicting microbial growth, phenanthrene depletion and biopolymer production. Furthermore, the Monod kinetic coefficients were largely determined by the surfactant-enhanced partition, suggesting that partitioning is a critical process in surfactant-enhanced bioremediation of hydro- phobic organic compounds. 展开更多
关键词 Phenanthrene Biodegradation Surfactant Kinetics Citrobacter sp.
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Citrobacter sp. CK3对活性红KN-3B的脱色反应动力学研究 认领 引用
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作者 赖正 熊小京 +2 位作者 郑天凌 林光辉 张章堂 《环境工程学报》 CAS CSCD 北大核心 2012年第8期2892-2896,共5页
采用脱色菌Citrobacter sp. CK3,以活性红KN-3B染料为处理对象,在厌氧批式反应条件下,系统考察了pH值,温度和染料浓度对脱色反应速度的影响;通过动力学模拟及反应过程中染料的UV-Vis扫描图分析,探讨了脱色反应机理。结果表明:Citrobacte... 采用脱色菌Citrobacter sp. CK3,以活性红KN-3B染料为处理对象,在厌氧批式反应条件下,系统考察了pH值,温度和染料浓度对脱色反应速度的影响;通过动力学模拟及反应过程中染料的UV-Vis扫描图分析,探讨了脱色反应机理。结果表明:Citrobacter sp. CK3对活性红KN-3B的脱色反应的适宜pH为7～9;脱色反应速度在温度为32℃时达到最大。染料初浓度从57 mg/L逐渐增大到458 mg/L时脱色率逐渐降低。脱色过程中染料的偶氮键发生断裂,脱色反应符合二级反应动力学。 展开更多
关键词 Citrobacter sp. CK3 活性红R180 脱色反应 动力学模拟
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Infective crystalline keratopathy due to Citrobacter koseri 认领 引用
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作者 Lim Thiam-Hou Evelyn Li Min Tai +1 位作者 Shamel Sonny Teo Khairy Ibrahim Mohtar 《Journal of Acute Disease》 2021年第1期36-38,共3页
Rationale:Infectious keratopathy is an ocular emergency with the potential to cause irreversible blindness.Patient's concerns:A 63-year-old diabetic man presented with a 3-day history of painful red right eye.He h... Rationale:Infectious keratopathy is an ocular emergency with the potential to cause irreversible blindness.Patient's concerns:A 63-year-old diabetic man presented with a 3-day history of painful red right eye.He had a history of multiple ocular surgeries in the affected eye without recent ocular trauma.Diagnosis:Infective crystalline keratopathy secondary to Citrobacter koseri.Interventions:Topical corticosteroids were discontinued,and dual topical antibiotic therapy of moxifloxacin 5%and gentamycin 0.3%were applied.Outcomes:Twelve hours after the start of treatment,the crystalline nature of the infiltrate disappeared,with enlargement of the epithelial defect.The antibiotic regime was continued and the lesion healed within a week of presentation with residual scarring.Lessons:Infective crystalline keratopathy classically presents with intrastromal branching fern-like opacities and minimal anterior segment inflammation in an immunosuppressed eye.The mainstay of management is corticosteroid discontinuation and the administration of empirical antibiotics until the results of the culture and sensitivity are available.In Citrobacter-related cases,treatment may result in a suppurative appearance before healing of the lesion. 展开更多
关键词 Infectious crystalline keratopathy Citrobacter koseri Gram-negative bacteria
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Citrobacter rodentium, a Gut Pathogen: The Yin and the Yang of Its Pathophysiology, Immunity and Clinical Manifestation in Mice 认领 引用
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作者 Tania Rahman Md. Ferdous Seraj Md. Monirul Islam 《Advances in Microbiology》 CAS 2018年第9期699-718,共20页
Pathogenic strains of E. coli including enteropathogenic E. coli (EPEC), enterohemorrhagic E. coli (EHEC), enterotoxigenic E. coli (ETEC) are principle causes for diarrhoea in many parts of the globe. Citrobacter rode... Pathogenic strains of E. coli including enteropathogenic E. coli (EPEC), enterohemorrhagic E. coli (EHEC), enterotoxigenic E. coli (ETEC) are principle causes for diarrhoea in many parts of the globe. Citrobacter rodentium (C. rodentium), a gram negative bacterium, is a murine pathogen that also utilizes type III secretion system and similar virulence factors to EPEC and EHEC and forms comparable attaching/effacing lesions in the intestines as EPEC and EHEC. The infection caused by C. rodentium in mice is usually self-limiting and results in only minor systemic effects with higher mortality in some susceptible mouse strains. All these characteristics have made the bacteria a commonly used model to study host immune responses to pathogenic E. coli infection. In this review, we focus on the impact of virulence factors of the pathogen;different immune components involved in the immune response and summarize their role during C. rodentium infection. 展开更多
关键词 Citrobacter rodentium Attaching and Effacing Pathogen Locus of Enterocyte Effacement Transmissible Murine Colonic Hyperplasia Colitis Mucosal Immune Response
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Isolation,Identification and Antibiotics Susceptibility Test of Citrobacter freundii from Procambarus clarkia 认领 引用 被引量：1
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作者 Chen Honglian Song Guangtong +2 位作者 He Jixiang Hou Guanjun Wang Yongjie 《Animal Husbandry and Feed Science》 CAS 2014年第5期238-241,共4页
This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia. Pathogenic bacteria from hepatopancreas of the diseased P. clarkia were examined using convention... This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia. Pathogenic bacteria from hepatopancreas of the diseased P. clarkia were examined using conventional methods,and then were isolated. The further tests and analysis of the isolated strain were developed,including the regression experiment to P. clarkia,the morphology,physiological and biochemical characteristics,sequence analysis of their 16 S rRNA and gyr B genes,and the susceptibility test to antibiotics. Large colonies with similar morphology and color were obtained. Strain X120523 was identified as Citrobacter freundii,proved to have strong pathogenicity,and was susceptible to quinolones and aminoglycosides. 展开更多
关键词 Procambarus clarkia Isolation and identification Citrobacter freundii 16S rRNA gene gyr B gene Antibiotics susceptibility test
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Evaluation of the Antimicrobial Activity of Stryphnodendron barbatiman against Citrobacter freundii 认领 引用
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作者 Nara Cde Souza Marcio N.Gomes +7 位作者 Rafael R.G.Maciel Romário Jda Silva Tarquin F.Trescher Filipe D.S.Gorza Graciela C.Pedro Kennedy C.S.Correa Marcio C.R.Souza Josmary R.Silva 《Materials Sciences and Applications》 2013年第12期780-785,共6页
Medicinal plants have been presented as a valuable source of preservation of human health. In special, Stryphnodendron barbatiman has been employed due to its antimicrobial activity. This plant is rich in tannins and ... Medicinal plants have been presented as a valuable source of preservation of human health. In special, Stryphnodendron barbatiman has been employed due to its antimicrobial activity. This plant is rich in tannins and has been used in popular medicine for the treatment of gastrointestinal disorder, treatment of lesions, and also as anti-inflammatory microbicide. Citrobacter freundii is a member of the family Enterobacteriaceae and is one of the major causes of opportunistic infections. This microorganism is a bacterium (bacillus) aerobic gram-negative with a length in the range of 1 to 5 mm. C. freundii is commonly found in water, soil, food and occasionally in the gastrointestinal tract of animals and humans. In this paper, we have demonstrated the antibacterial activity of S. barbatiman by observing cellular death by using inhibition halo approach. Atomic force microscopy and FT-IR spectroscopy results suggested that interaction between the main active components of S. barbatiman with cellular wall of C. freundii gives rise to cellular wall damage, and then leads this microorganism to death. 展开更多
关键词 Stryphnodendron barbatiman Citrobacter freundii Atomic Force Microscopy Antimicrobial Activity
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Letter to the Editor:Probiotics counteract polyethylene glycol bowel preparation dysbiosis and infection risk 认领 引用
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作者 Yang Fu 《World Journal of Gastroenterology》 SCIE CAS 2026年第28期157-160,F0003,共4页
Polyethylene glycol(PEG)bowel preparation,though widely used,can significantly disrupt gut microbiota composition.Kou et al recently published study in the World Journal of Gastroenterology,which demonstrated that PEG... Polyethylene glycol(PEG)bowel preparation,though widely used,can significantly disrupt gut microbiota composition.Kou et al recently published study in the World Journal of Gastroenterology,which demonstrated that PEG-treated mice challenged with Citrobacter rodentium exhibit higher pathogen loads,elevated virulence gene expression,and worsened colitis,mediated by microbiota dysbiosis.Importantly,Lactobacillus acidophilus supplementation restored microbial balance and mitigated infection severity,suggesting PEG-induced loss of colonization resistance exposes a 14-day infection window.This paradigm aligns with a rare human case of septic shock after PEG bowel preparation,highlighting potential opportunistic infection risk.Probiotic Lactobacillus strengthen the epithelial barrier and modulate immunity,and clinical trials support their efficacy and safety in gastrointestinal contexts.We discuss the translational significance of these findings and advocate microbiota-protective strategies(probiotic supplementation,optimal timing of endoscopy,and dietary adjustments)during bowel preparation. 展开更多
关键词 Polyethylene glycol Bowel preparation Dysbiosis Citrobacter rodentium Probiotics Colonoscopy Infection risk
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Immobilization of Lactobacillus plantarum NCIMB 8826 ameliorates Citrobacter rodentium induced lesions and enhances the gut inflammatory response in C57BL/6 weanling mice 认领 引用 被引量：1
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作者 Emmanuel Kyereh Diana Coulon +3 位作者 Ingeborg Langohr Anne Raggio Michael Keenan Subramaniam Sathivel 《Food Production, Processing and Nutrition》 CAS 2022年第1期447-458,共12页
Infectious diarrhea is a major cause of infant mortality in most developing countries. In this research, we evaluated the potential of immobilized Lactobacillus plantarum NCIMB 8826 on weanimix infant cereal and its e... Infectious diarrhea is a major cause of infant mortality in most developing countries. In this research, we evaluated the potential of immobilized Lactobacillus plantarum NCIMB 8826 on weanimix infant cereal and its effectiveness in reducing the severity of Citrobacter rodentium-induced diarrhea in weanling mice. Thirty-six C57BL/6 weanling mice were placed into four groups (n = 9 each;negative, positive, prevention and cure). Mice received either L. plantarum (109 CFU/g) immobilized on weanimix infant cereal 3 days before C. rodentium (109 CFU/ ml) infection (Prevention) or 3 days after C. rodentium infection (Cure). A positive control group was infected with C. rodentium only, while a nega-tive control group received neither L. plantarum nor C. rodentium. Positive control mice showed colonic mucosal and submucosal inflammation, erosion, and mucosal epithelia hyperplasia with the C. rodentium infection. Mice in the prevention and cure groups had less severe histologic alterations in the colon. Some beneficial effect of L. plantarum was observed in cecal short-chain fatty acid concentrations, which stimulates water and electrolytes absorption to reduce diarrhea. Our findings demonstrated that L. plantarum NCIMB 8826 could be immobilized on weanimix infant cereal to help reduce diarrhea during weaning. 展开更多
关键词 Probiotics Citrobacter rodentium Lactobacillus plantarum Inflammation Weanimix
Citrobacter Causing Septic Arthritis: A Case Report 认领 引用
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作者 Kimberly K.Levine Mary Dahl +1 位作者 Abubakar Tauseef Joseph Thirumalareddy 《Infectious Diseases & Immunity》 CSCD 2024年第3期146-148,共3页
The genus Citrobacter commonly found in water and soil as well as the intestinal and urinary tracts of animals and humans.Although Citrobacter infections are infrequently diagnosed in environmental contexts,the rising... The genus Citrobacter commonly found in water and soil as well as the intestinal and urinary tracts of animals and humans.Although Citrobacter infections are infrequently diagnosed in environmental contexts,the rising incidence of nosocomial manifestations underscores the importance of considering this organism in the differential diagnosis of hospital-acquired infections.We present a case of patient with septic arthritis of the shoulder who had been originally admitted for the management of acute-on-chronic congestive heart failure exacerbation and severe sepsis.On hospital day 1,joint aspiration cultures and Gram stain confirmed the presence of Citrobacter koseri,a pathogen not commonly associated with septic arthritis,and ceftriaxone was administered to target it.On hospital day 7,antibiotic was transitioned to levofloxacin,every other day for 4 weeks.The patient was discharged to an acute rehabilitation facility after a 14-day hospital stay.This case stresses the importance of maintaining a broad differential diagnosis for any patients who present with fever and joint inflammation or swelling,especially in a postoperative setting. 展开更多
关键词 Citrobacter koseri Bacteremia Septic arthritis
克氏原螯虾弗氏柠檬酸杆菌的分离鉴定与药敏试验 认领 引用 被引量：27
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作者 陈红莲 宋光同 +2 位作者 何吉祥 侯冠军 王永杰 《淡水渔业》 CSCD 北大核心 2014年第1期73-77,共5页
为确定引起养殖克氏原螯虾(Procambarus clarkii)患病的病原种类及耐药情况,从患病克氏原螯虾肝胰腺取样进行常规细菌学检查,对分离株进行回归感染试验、形态观察、生理生化特性检测、16S rRNA和gyrB基因分析及药敏试验。结果显示:从患... 为确定引起养殖克氏原螯虾(Procambarus clarkii)患病的病原种类及耐药情况,从患病克氏原螯虾肝胰腺取样进行常规细菌学检查,对分离株进行回归感染试验、形态观察、生理生化特性检测、16S rRNA和gyrB基因分析及药敏试验。结果显示:从患病克氏原螯虾肝胰腺分离到大量菌落形态和色泽一致的细菌,代表菌株X120523为弗氏柠檬酸杆菌(Citrobacter freundii),具有较强致病性,对20种抗生素中的喹诺酮类和氨基糖苷类抗生素敏感。 展开更多
关键词 克氏原螯虾(Procambarus clarkii) 弗氏柠檬酸杆菌(Citrobacter freundii) 分离鉴定 16S rRNA基因 gyrB基因 药敏试验
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41种中草药对3种鲟源病原菌的体外抑菌效果 认领 引用 被引量：16
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作者 杨移斌 曹海鹏 +4 位作者 夏永涛 杨婷 赵蕾 胡鲲 杨先乐 《淡水渔业》 CSCD 北大核心 2013年第4期80-84,共5页
采用平板打孔法在观察了弗氏柠檬酸杆菌(Citrobacter freundii)、鲁氏耶尔森氏菌(Yersinia ruckeri)、嗜水气单胞菌(Aeromonas hydrophila)等3种鲟源病原菌对41种中草药敏感性的基础上,进一步采用二倍稀释法测定了具有良好抑菌活性的中... 采用平板打孔法在观察了弗氏柠檬酸杆菌(Citrobacter freundii)、鲁氏耶尔森氏菌(Yersinia ruckeri)、嗜水气单胞菌(Aeromonas hydrophila)等3种鲟源病原菌对41种中草药敏感性的基础上,进一步采用二倍稀释法测定了具有良好抑菌活性的中草药对3种病原菌的最小抑菌浓度和最小杀菌浓度。实验结果表明,3种病原菌对乌梅、石榴皮、地榆、杞子等均高度敏感,对杜仲、柴胡、丹参、熟地黄等均中度敏感,对麦冬、当归、胖大海、玉竹均不敏感。此外,乌梅、石榴皮、地榆、杞子对3种病原菌的最小抑菌浓度和最小杀菌浓度分别为7.81 mg/mL和15.625 mg/mL、15.625 mg/mL和31.25 mg/mL、15.625 mg/mL和31.25 mg/mL以及15.625 mg/mL和31.25 mg/mL,而杜仲、柴胡、丹参、熟地黄等中度敏感中草药对3种病原菌的最小抑菌浓度和最小杀菌浓度均分别高于31.25 mg/mL和62.5 mg/mL。 展开更多
关键词 鲟(Acipenser sp.) 弗氏柠檬酸杆菌(Citrobacter freundii) 鲁氏耶尔森氏菌(Yersinia ruckeri) 嗜水气单胞菌(Aeromonas hydrophila) 中草药 抑菌效果
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大鲵源致病性弗氏柠檬酸杆菌的分离、鉴定和药物敏感性 认领 引用 被引量：15
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作者 苏英 吴荣华 +4 位作者 申君宇 李伟平 何滔 李云 丁诗华 《淡水渔业》 CSCD 北大核心 2019年第4期56-61,共6页
为鉴定患病大鲵(Andrias davidianus)病原菌,从大鲵肠、胃、脾脏中分离得到菌株int1705,分离菌株经形态特征观察、生理生化特性测定、16S rRNA和aspC管家基因序列测定及系统发育学分析,鉴定为弗氏柠檬酸杆菌(Citrobacter freundii)。经... 为鉴定患病大鲵(Andrias davidianus)病原菌,从大鲵肠、胃、脾脏中分离得到菌株int1705,分离菌株经形态特征观察、生理生化特性测定、16S rRNA和aspC管家基因序列测定及系统发育学分析,鉴定为弗氏柠檬酸杆菌(Citrobacter freundii)。经人工感染,结果显示:菌株int1705能使健康大鲵发病并致死,半致死浓度(LD50)为3.16×10 6 CFU/mL,且发病症状与自然发病相似。药敏试验结果显示,菌株int1705对多粘菌素B、头孢他啶、诺氟沙星、氧氟沙星、复方新诺明等10种药物敏感。 展开更多
关键词 大鲵(Andrias davidianus) 弗氏柠檬酸杆菌(Citrobacter freundii) 致病性 药敏试验
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虹鳟弗氏柠檬酸杆菌的分离鉴定与多位点序列分型 认领 引用 被引量：11
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作者 张明洋 胡安东 +2 位作者 程振涛 姜海波 文明 《基因组学与应用生物学》 CAS CSCD 北大核心 2020年第6期2521-2528,共8页
本研究旨在对虹鳟体内分离菌进行分子鉴定分型及耐药性分析。通过形态学观察、生理生化反应、16S rRNA基因比对进化分析及管家基因多位点序列分型等方法,确定分离菌的种属地位、分析种内分子变异特征;利用纸片扩散法与PCR方法,分析分离... 本研究旨在对虹鳟体内分离菌进行分子鉴定分型及耐药性分析。通过形态学观察、生理生化反应、16S rRNA基因比对进化分析及管家基因多位点序列分型等方法,确定分离菌的种属地位、分析种内分子变异特征;利用纸片扩散法与PCR方法,分析分离菌耐药表型与基因型之间关系;进行人工感染小鼠试验,评估分离菌致病力强弱。结果显示,分离菌(GZBJ2017)为革兰氏阴性杆菌;16S rRNA基因与弗氏柠檬酸杆菌16S r RNA基因同源性达95.4%,结合生理生化反应结果,确定分离菌为弗氏柠檬酸杆菌;分离菌7个管家基因均发生变异,多位点序列分型鉴定为新序列型,与标准菌株ST-132亲缘关系最近;分离菌对头孢曲松、多粘菌素B、环丙沙星、氧氟沙星4种药物敏感,对四环素、庆大霉素、泰乐菌素、复方新诺明、青霉素、卡那霉素6种药物耐药;分离菌携带Sul1、Sul2、Intl1等3种耐药基因,与耐药表型相符;人工感染试验结果显示,该菌对小鼠具有一定的致病力。本研究对本次虹鳟发病的病因作出了准确诊断,为虹鳟弗氏柠檬酸杆菌感染的防治与分子流行病学研究提供依据。 展开更多
关键词 虹鳟(Oncorhynchus mykiss) 弗氏柠檬酸杆菌(Citrobacter freundii) 分离鉴定 多位点序列分型 耐药基因 药敏试验
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