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ZmL75 is required for colonization by arbuscular mycorrhizal fungi and for saline-alkali tolerance in maize 认领 引用 被引量：3
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作者 Jie Liu Boming Yang +9 位作者 Xunji Chen Tengfei Zhang Huairen Zhang Yimo Du Qian Zhao Zhaogui Zhang Darun Cai Juan Liu Huabang Chen Li Zhao 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2025年第3期334-345,共12页
Saline-alkali soil severely reduces the productivity of crops,including maize(Zea mays).Although several genes associated with saline-alkali tolerance have been identified in maize,the underlying regulatory mechanism ... Saline-alkali soil severely reduces the productivity of crops,including maize(Zea mays).Although several genes associated with saline-alkali tolerance have been identified in maize,the underlying regulatory mechanism remains elusive.Here,we report a direct link between colonization by arbuscular mycorrhizal fungi(AMF)and saline-alkali tolerance in maize.We identify s75,a natural maize mutant that cannot survive under moderate saline-alkali soil conditions or establish AM symbioses.The saline-alkali hypersensitive phenotype of s75 is caused by a 1340-bp deletion in Zm00001d033915,designated as ZmL75.This gene encodes a glycerol-3-phosphate acyltransferase localized in the endoplasmic reticulum,and is responsible for AMF colonization.ZmL75 expression levels in roots correspond with the root length colonization(RLC)rate during early vegetative development.Notably,the s75 mutant line shows a complete loss of AMF colonization,along with alterations in the diversity and structure of its root fungal microbiota.Conversely,overexpression of ZmL75 increases the RLC rate and enhances tolerance to saline-alkali soil conditions.These results suggest that ZmL75 is required for symbiosis with AMF,which directly improves saline-alkali tolerance.Our findings provide insights into maize-AMF interactions and offer a potential strategy for maize improvement. 展开更多
关键词 Maize Saline-alkali ZmL75 AMF colonization Tolerance
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Community dynamics during de novo colonization of the nascent peri-implant sulcus 认领 引用 被引量：2
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作者 Tamires Pereira Dutra Nicolas Robitaille +2 位作者 Khaled Altabtbaei Shareef M.Dabdoub Purnima S.Kumar 《International Journal of Oral Science》 SCIE CAS CSCD 2025年第3期361-367,共7页
Dental implants have restored masticatory function to over 100000000 individuals,yet almost 1000000 implants fail each year due to peri-implantitis,a disease triggered by peri-implant microbial dysbiosis.Our ability t... Dental implants have restored masticatory function to over 100000000 individuals,yet almost 1000000 implants fail each year due to peri-implantitis,a disease triggered by peri-implant microbial dysbiosis.Our ability to prevent and treat peri-implantitis is hampered by a paucity of knowledge of how these biomes are acquired and the factors that engender normobiosis.Therefore,we combined a 3-month interventional study of 15 systemically and periodontally healthy adults with whole genome sequencing,finescale enumeration and graph theoretics to interrogate colonization dynamics in the pristine peri-implant sulcus.We discovered that colonization trajectories of implants differ substantially from adjoining teeth in acquisition of new members and development of functional synergies.Source-tracking algorithms revealed that this niche is initially seeded by bacteria trapped within the coverscrew chamber during implant placement.These pioneer species stably colonize the microbiome and exert a sustained influence on the ecosystem by serving as anchors of influential hubs and by providing functions that enable cell replication and biofilm maturation.Unlike the periodontal microbiome,recruitment of new members to the peri-implant community occurs on nepotistic principles.Maturation is accompanied by a progressive increase in anaerobiosis,however,the predominant functionalities are oxygen-dependent over the 12-weeks.The peri-implant community is easily perturbed following crown placement,but demonstrates remarkable resilience;returning to pre-perturbation states within three weeks.This study highlights important differences in the development of the periodontal and peri-implant ecosystems,and signposts the importance of placing implants in periodontally healthy individuals or following the successful resolution of periodontal disease. 展开更多
关键词 whole genome sequencingfinescale enu masticatory function microbial dysbiosis de novo colonization peri implantitis dental implants peri implant sulcus whole genome sequencing
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Inoculation and Colonization:British Medical Activities in Xizang and Their Significance(1900-1907) 认领 引用
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作者 TANG Yiyun 《Cultural and Religious Studies》 2025年第3期131-134,共4页
In the early years of the 20th century,Britain sought to compete with Russia for dominance in East Asia,and set its sights on the strategic location of Xizang.Smallpox virus epidemics have been documented in Xizang si... In the early years of the 20th century,Britain sought to compete with Russia for dominance in East Asia,and set its sights on the strategic location of Xizang.Smallpox virus epidemics have been documented in Xizang since the 7th century,and smallpox remained a major public health issue in Xizang until the early 20th century.The British colonizers learned from their experience of passing on biological science and technology in India and carried out a series of medical activities in Xizang,the most influential of which was the smallpox vaccination for the people of Gyantse and other places.This paper examines the history of the fight against smallpox in Xizang from 1900 to 1907,and studies the historical process of British colonial expansion in Xizang,as well as the interaction between the Qing government and the British invaders in medical and healthcare events such as vaccination in Xizang. 展开更多
关键词 smallpox vaccination British colonization Xizang Qing government medical diplomacy
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Colonization and development of the gut microbiome in calves 认领 引用 被引量：14
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作者 Yufeng Du Ya Gao +6 位作者 Mingyang Hu Jinxiu Hou Linhai Yang Xianghuang Wang Wenjuan Du Jianxin Liu Qingbiao Xu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第4期1329-1343,共15页
Colonization and development of the gut microbiome are crucial for the growth and health of calves.In this review,we summarized the colonization,beneficial nutrition,immune function of gut microbiota,function of the g... Colonization and development of the gut microbiome are crucial for the growth and health of calves.In this review,we summarized the colonization,beneficial nutrition,immune function of gut microbiota,function of the gut barrier,and the evolution of core microbiota in the gut of calves of different ages.Homeostasis of gut microbiome is beneficial for nutritional and immune system development of calves.Disruption of the gut microbiome leads to digestive diseases in calves,such as diarrhea and intestinal inflammation.Microbiota already exists in the gut of calf fetuses,and the colonization of microbiota continues to change dynamically under the influence of various factors,which include probiotics,diet,age,and genotype.Colonization depends on the interaction between the gut microbiota and the immune system of calves.The abundance and diversity of these commensal microbiota stabilize and play a critical role in the health of calves. 展开更多
关键词 Calf Colonization Development Gut health Gut microbiome
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Natural Colonization of Rice by Arbuscular Mycorrhizal Fungi in Different Production Areas 认领 引用 被引量：9
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作者 Lina BERNAOLA Grace CANGE +3 位作者 Michael O.WAY Jeffrey GORE Jarrod HARDKE Michael STOUT 《Rice science》 SCIE CSCD 2018年第3期169-174,共6页
Interactions between plants and soil microorganisms can influence the other interactions in which plants participate, including interactions with herbivores. Many fungi, including arbuscular mycorrhizal fungi(AMF), fo... Interactions between plants and soil microorganisms can influence the other interactions in which plants participate, including interactions with herbivores. Many fungi, including arbuscular mycorrhizal fungi(AMF), form symbiotic relationships with the roots they inhabit, and potentially alter defense against pests. The objective of this study was to document the extent of root colonization by AMF on non-flooded rice plants grown under conditions typical of commercial fields. We hypothesized that AMF naturally colonized rice plants in different rice producing field locations. Rice plant samples were collected from areas across the southern United States, including Texas, Mississippi, Arkansas and two research stations in Louisiana. We quantified the amount of AMF colonization in insecticide-free rice plants over three consecutive years(2014–2016). The results revealed natural colonization of AMF in all rice producing areas. In all the three years of survey, rice-AMF associations were the greatest in Arkansas followed by Mississippi and Texas. This research will help draw attention to natural colonization of AMF in rice producing areas that can impact future rice research and production by facilitating agricultural exploitation of the symbiosis. 展开更多
关键词 arbuscular mycorrhizal fungus rice root colonization soil quality agriculture
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Characterization of flgK gene and FlgK protein required for H pylori Colonization-from cloning to clinical relevance 认领 引用 被引量：5
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作者 Jiunn-Jong Wu Bor-Shyang Sheu +2 位作者 Ay-Huey Huang Shin-Ting Lin Hsiao-Bai Yang 《World Journal of Gastroenterology》 SCIE CAS 2006年第25期3989-3993,共5页
AIM： To characterize the role of flgK and its protein product in Hpylori colonization. METHODS： The PCR cloning method identified the flgK gene. An isogenic flgK mutant was constructed by gene replacement and confir... AIM： To characterize the role of flgK and its protein product in Hpylori colonization. METHODS： The PCR cloning method identified the flgK gene. An isogenic flgK mutant was constructed by gene replacement and confirmed by Southern blot analysis and PCR analysis. The recombinant FlgK protein （r-FlgK） was purified. Electron microscopy （EM） was applied to demonstrate the flagella of H pylori. An in vitro motility test was assessed in semisolid medium. The densities of H pylori colonization with either the wild-type strain or its flgK mutant were compared among BALB/c mice with or without pre-immunization with r-FlgK. The serological responses to r-FlgK were analyzed for 70 clinical patients with different densities of H pylori colonization. RESULTS： From a duodenal ulcer strain, the flgK gene was cloned and it contained 1821 bp, with a 95.7% identity to the published sequences. No flagella were observed under EM for the mutant strain, which had a loss of motility. Hpylori density was lower in the BALB/c mice inoculated by the mutant or with pre-immunization with r-FlgK compared to unimmunized mice or mice inoculated by the wild-type strain （P 〈 0.05）. In the H pylori-infected patients, the serological responses to r-FlgK were uniformly low in titer.CONCLUSION： FlgK encoded by flgK is important for flagella formation and H pylori motility. Deficiency in FlgK or an enhanced serological response to r-FlgK can interfere with Hpylori colonization. FlgK of Hpylori could be a novel target for vaccination. 展开更多
关键词 H pylori Colonization Isogenic mutant BALB/c mice Flagella Vaccine.
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Helicobacter pylori intragastric colonization and migration:Endoscopic manifestations and potential mechanisms 认领 引用 被引量：5
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作者 Tong Mu Zhi-Ming Lu +4 位作者 Wen-Wen Wang Hua Feng Yan Jin Qian Ding Li-Fen Wang 《World Journal of Gastroenterology》 SCIE CAS 2023年第30期4616-4627,共12页
After being ingested and entering the human stomach,Helicobacter pylori(H.pylori)adopts several effective strategies to adhere to and colonize the gastric mucosa and move to different regions of the stomach to obtain ... After being ingested and entering the human stomach,Helicobacter pylori(H.pylori)adopts several effective strategies to adhere to and colonize the gastric mucosa and move to different regions of the stomach to obtain more nutrients and escape from the harsher environments of the stomach,leading to acute infection and chronic gastritis,which is the basis of malignant gastric tumors.The endoscopic manifestations and pathological features of H.pylori infection are diverse and vary with the duration of infection.In this review,we describe the endoscopic manifestations of each stage of H.pylori gastritis and then reveal the potential mechanisms of bacterial intragastric colonization and migration from the perspective of endoscopists to provide direction for future research on the effective therapy and management of H.pylori infection. 展开更多
关键词 Helicobacter pylori Colonization Endoscopy Gastritis Infection
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Factors that mediate colonization of the human stomach by Helicobacter pylori 认领 引用 被引量：10
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作者 Ciara Dunne Brendan Dolan Marguerite Clyne 《World Journal of Gastroenterology》 SCIE CAS 2014年第19期5610-5624,共15页
Helicobacter pylori(H.pylori)colonizes the stomach of humans and causes chronic infection.The majority of bacteria live in the mucus layer overlying the gastric epithelial cells and only a small proportion of bacteria... Helicobacter pylori(H.pylori)colonizes the stomach of humans and causes chronic infection.The majority of bacteria live in the mucus layer overlying the gastric epithelial cells and only a small proportion of bacteria are found interacting with the epithelial cells.The bacteria living in the gastric mucus may act as a reservoir of infection for the underlying cells which is essential for the development of disease.Colonization of gastric mucus is likely to be key to the establishment of chronic infection.How H.pylori manages to colonise and survive in the hostile environment of the human stomach and avoid removal by mucus flow and killing by gastric acid is the subject of this review.We also discuss how bacterial and host factors may together go some way to explaining the susceptibility to colonization and the outcome of infection in different individuals.H.pylori infection of the gastric mucosa has become a paradigm for chronic infection.Understanding of why H.pylori is such a successful pathogen may help us understand how other bacterial species colonise mucosal surfaces and cause disease. 展开更多
关键词 Helicobacter pylori Colonization Infection Gastric mucosa Urease Flagella Polymorphisms Adhesins CagA Type IV secretion system
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The odd one out or a hidden generalist:Hawaiian Melicope (Rutaceae)do not share traits associated with successful island colonization 认领 引用 被引量：3
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作者 Claudia Paetzold Michael Kiehn +2 位作者 Kenneth R.Wood Warren L.Wagner Marc S.Appelhans 《Journal of Systematics and Evolution》 SCIE CAS CSCD 2018年第6期621-636,共16页
Oceanic islands are unique in their species composition,which is defined by arrival of colonizers via long distance dispersal followed by establishment of species followed in some cases by adaptive radiation.Evolution... Oceanic islands are unique in their species composition,which is defined by arrival of colonizers via long distance dispersal followed by establishment of species followed in some cases by adaptive radiation.Evolutionary biologists identified traits facilitating successful colonization of islands as including polyploidy,self-compatibility, herbaceousness and ability for long-distance dispersal.Successful establishment and evolutionary diversification of lineages on islands often involves shifts to woodiness and shifts in methods of outcrossing as well as changes in dispersal ability.The genus Meflicope colonized numerous archipelagos throughout the Pacific including the Hawaiian Islands,where the lineage comprises currently 54endemic species and represents the largest radiation of woody plants on the islands.The wide distributional range of the genus illustrates its high dispersibility,most likely, due to adaption to bird dispersal.Here we investigate ploidy in the genus using flow cytometry and chromosome counting.We find the genus to be paleopolyploid with 2n =4x =36,a ploidy level characterizing the entire subfamily Amyridoideae and dating back to at least the Palaeocene.Therefore Hawaiian MeHcope have not undergone recent polyploidization prior to colonization of the islands.Thus Nlelicope retained colonization success while exhibiting a combination of traits that typically characterize well established island specialists while lacking some traits associated to successful colonizers. 展开更多
关键词 bird dispersal colonization establishment long distance dispersal Pacific polyploidy
Mycorrhizal colonization and distribution of arbuscular mycorrhizal fungi associated with Michelia champaca L.under plantation system in northeast India 认领 引用 被引量：1
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作者 Das Panna Kayang Highland 《Journal of Forestry Research》 CAS 2010年第2期137-142,I0003,I0004,共6页
Arbuscular mycorrhizal fungi（AMF） and dark septate endophyte（DSE） colonization were investigated in three different plantation sites（Umdihar,Umsaw and Mawlein） of Meghalaya,northeast India.Isolation and identifi... Arbuscular mycorrhizal fungi（AMF） and dark septate endophyte（DSE） colonization were investigated in three different plantation sites（Umdihar,Umsaw and Mawlein） of Meghalaya,northeast India.Isolation and identification of the AMF spore were conducted to evaluate the AMF diversity and host preference in terms of AMF species distribution and abundance in the plantation sites.Results showed that AMF colonization was significantly higher than dark septate endophyte colonization（p〉0.05）.AMF and DSE colonization had a narrow range of colonization,varying from 50.91%-58.95% and 1.84%-4.11%,respectively.Spore density varied significantly in all the sites（p〉0.05）.Out of 29 species identified from 7 genera,the species from Glomus was found to be highly abundant.Sorenson coefficient（Cs） ranged from 0.35-7.0.Species richness varied from 2.0-2.9 in the sites.Total species richness was significantly correlated with total relative abundance（p=0.001）.The distribution,abundance and principal component analysis plot suggest that Glomus macrocarpum,G.multicaulis,G.constrictum and Acaulospora sp 1 were the most host preferred species which possibly may favour the host with proper nutrient acquisition and growth. 展开更多
关键词 arbuscular mycorrhizal colonization dark septate endophyte colonization Glomus Michelia champaca
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Biofilm-overproducing Bacillus subtilis B12ΔYwcc decreases Cd uptake in Chinese cabbage through increasing Cd-immobilizing related gene abundance and root surface colonization 认领 引用 被引量：4
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作者 Yanyan Ge Zhanbiao Ge +2 位作者 Jinwei Zheng Xiafang Sheng Linyan He 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2022年第10期84-93,共10页
Biofilm-producing bacteria can decrease Cd uptake in vegetables, but mechanisms underlying this effect are poorly characterized. In this study, two mutant strains B12ΔYwcc and B12ΔSlr R were constructed from a biofi... Biofilm-producing bacteria can decrease Cd uptake in vegetables, but mechanisms underlying this effect are poorly characterized. In this study, two mutant strains B12ΔYwcc and B12ΔSlr R were constructed from a biofilm-producing Bacillus subtilis strain B12. Then, the impacts of strain B12 and its high biofilm-producing mutant strain B12ΔYwcc and low biofilmproducing mutant strain B12ΔSlr R on Cd availability and uptake in Chinese cabbage and the related mechanisms were investigated in the Cd-polluted soil. Strain B12 and its mutants B12ΔYwcc and B12ΔSlr R increased the dry biomasses of edible tissues by 54%–130% compared with the controls. Strain B12 and its mutant B12ΔYwcc reduced the soil available Cd content by 36%–50% and root and edible tissue Cd contents by 23%–50% compared with the controls. Furthermore, the mutant strain B12ΔYwcc reduced the edible tissue Cd content by40% and increased the polysaccharide content by 23%, invertase activity by 139%, and gene copies of the cum A by 4.5-fold, eps A by 7.1-fold, and cad A by 4.3-fold, which were involved in Cd adsorption in the rhizosphere soils, respectively, compared with strain B12. The polysaccharide content and cum A, eps A, and cad A gene copy numbers showed significantly reverse correlations with the available Cd content. Notably, the mutant strain B12ΔYwcc showed better ability to colonize the vegetable root surface than strain B12. These findings demonstrated that the biofilm-overproducing mutant strain B12ΔYwcc increased the polysaccharide production and Cd-immobilizing related cum A, eps A, and cad A gene copies, resulting in lower Cd availability and accumulation in Chinese cabbage in the Cd-polluted soil. 展开更多
关键词 Biofilm-producing Bacillus subtilis Mutant strain with biofilm overproduction Cd uptake in vegetable Cd-stabilizing related genes Bacterial colonization and biofilm formation
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From mainland to islands:colonization history in the tree frog Kurixalus(Anura:Rhacophoridae) 认领 引用 被引量：2
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作者 Guo-Hua Yu Li-Na Du +3 位作者 Ji-Shan Wang Ding-Qi Rao Zheng-Jun Wu Jun-Xing Yang 《Current Zoology》 SCIE CAS CSCD 2020年第6期667-675,共9页
The origin and colonization history of Kurixalus,a genus of small arboreal tree frogs breeding exclusively in shallow swamps,is under disputed.On the basis of comprehensive sampling program,the evolutionary history of... The origin and colonization history of Kurixalus,a genus of small arboreal tree frogs breeding exclusively in shallow swamps,is under disputed.On the basis of comprehensive sampling program,the evolutionary history of Kurixalus is investigated based on 3 mitochondrial genes.Our results indicate that the genus Kurixalus originated in the Asian mainland and subsequently arrived at its current distribution in Borneo,Taiwan,Ryukyu,and Hainan islands by a series of dispersal events.Moreover,the colonization of Taiwan from mainland Asia has occurred 2 times.The initial colonization of Taiwan occurred at 3.46-8.68 Mya(95%highest posterior density),which rejects the hypothesis that Kurixalus probably originated from Taiwan during the early Oligocene and favors the model of Neogene-origin rather than the model of Quaternary-origin for Taiwan Residents Kurixalus.Kurixalus eiffingeri has dispersed from Taiwan to the Ryukyus once or 2 times pending more data.Both transoceanic dispersal and landbridge dispersal have played a role in the colonization process;the former resulted in the colonization of Taiwan and the Ryukyus and the latter led to the colonizati on of Bor neo and Hain an. 展开更多
关键词 colonization Kurixalus landbridge Neogene-origin Taiwan Island transoceanic dispersal
Colonization by Klebsiella variicola FH-1 stimulates soybean growth and alleviates the stress of Sclerotinia sclerotiorum 认领 引用 被引量：2
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作者 ZHAI Qian-hang PAN Ze-qun +6 位作者 ZHANG Cheng YU Hui-lin ZHANG Meng GU Xue-hu ZHANG Xiang-hui PAN Hong-yu ZHANG Hao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第9期2729-2745,共17页
Sclerotinia stem rot,caused by Sclerotinia sclerotiorum,is a destructive soil-borne disease leading to huge yield loss.We previously reported that Klebsiella variicola FH-1 could degrade atrazine herbicides,and the ve... Sclerotinia stem rot,caused by Sclerotinia sclerotiorum,is a destructive soil-borne disease leading to huge yield loss.We previously reported that Klebsiella variicola FH-1 could degrade atrazine herbicides,and the vegetative growth of atrazine-sensitive crops(i.e.,soybean)was significantly increased in the FH-1-treated soil.Interestingly,we found that FH-1 could promote soybean growth and induce resistance to S.sclerotiorum.In our study,strain FH-1 could grow in a nitrogen-free environment,dissolve inorganic phosphorus and potassium,and produce indoleacetic acid and a siderophore.The results of pot experiments showed that K.variicola FH-1 promoted soybean plant development,substantially improving plant height,fresh weight,and root length,and induced resistance against S.sclerotiorum infection in soybean leaves.The area under the disease progression curve(AUDPC)for treatment with strain FH-1 was significantly lower than the control and was reduced by up to 42.2%within 48 h(P<0.001).Moreover,strain FH-1 rcovered the activities of catalase,superoxide dismutase,peroxidase,phenylalanine ammonia lyase,and polyphenol oxidase,which are involved in plant protection,and reduced malondialdehyde accumulation in the leaves.The mechanism of induction of resistance appeared to be primarily resulted from the enhancement of transcript levels of PR10,PR12,AOS,CHS,and PDF1.2 genes.The colonization of FH-1 on soybean root,determined using CLSM and SEM,revealed that FH-1 colonized soybean root surfaces,root hairs,and exodermis to form biofilms.In summary,K.variicola FH-1 exhibited the biological control potential by inducing resistance in soybean against S.sclerotiorum infection,providing new suggestions for green prevention and control. 展开更多
关键词 sclerotinia stem rot of soybean Klebsiella variicola FH-1 inducing resistance root colonization biofilm
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Non-thermal Plasma Suppresses Bacterial Colonization on Skin Wound and Promotes Wound Healing in Mice 认领 引用 被引量：6
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作者 俞莺 谭明 +9 位作者 陈宏翔 吴志洪 许莉 李娟 曹经江 杨寅生 肖学敏 连昕 卢新培 涂亚庭 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2011年第3期390-394,共5页
The present study evaluated the effect of non-thermal plasma on skin wound healing in BalB/c mice.Two 6-mm wounds along the both sides of the spine were created on the back of each mouse（n=80） by using a punch biops... The present study evaluated the effect of non-thermal plasma on skin wound healing in BalB/c mice.Two 6-mm wounds along the both sides of the spine were created on the back of each mouse（n=80） by using a punch biopsy.The mice were assigned randomly into two groups,with 40 animals in each group：a non-thermal plasma group in which the mice were treated with the non-thermal plasma;a control group in which the mice were left to heal naturally.Wound healing was evaluated on postoperative days（POD） 4,7,10 and 14（n=5 per group in each POD） by percentage of wound closure.The mice was euthanized on POD 1,4,7,10,14,21,28 and 35（n=1 in each POD）.The wounds were removed,routinely fixed,paraffin-embedded,sectioned and HE-stained.A modified scoring system was used to evaluate the wounds.The results showed that acute inflammation peaked on POD 4 in non-thermal plasma group,earlier than in control group in which acute inflammation reached a peak on POD 7,and the acute inflammation scores were much lower in non-thermal group than in control group on POD 7（P0.05）.The amount of granular tissue was greater on POD 4 and 7 in non-thermal group than in control group（P0.05）.The re-epithelialization score and the neovasularization score were increased significantly in non-thermal group when compared with control group on POD 7 and 10（P0.05 for all）.The count of bacterial colonies was 103 CFU/mL on POD 4 and 20 CFU/mL on POD 7,significantly lower than that in control group（109 CFU/mL on POD 4 and 1012 CFU/mL on the POD 7）（P0.05）.It was suggested that the non-thermal plasma facilitates the wound healing by suppressing bacterial colo-nization. 展开更多
关键词 non-thermal plasma wound healing BalB/c mice bacterial colonization
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Colonization and Probiotic Effect of Metschnikowia sp. C14 in the Intestine of Juvenile Sea Cucumber, Apostichopus japonicus 认领 引用 被引量：2
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作者 LI Ming BAO Pengyun +3 位作者 SONG Jian DING Jianfeng LIU Yubin MA Yuexin 《Journal of Ocean University of China》 SCIE CAS CSCD 2020年第1期225-231,共7页
Viable cell count was used to determine whether Metschnikowia sp.C14 can colonize the intestine of juvenile sea cucumber Apostichopus japonicus.Sea cucumber individuals were divided into two groups,which were fed the ... Viable cell count was used to determine whether Metschnikowia sp.C14 can colonize the intestine of juvenile sea cucumber Apostichopus japonicus.Sea cucumber individuals were divided into two groups,which were fed the control diet for 38 days or the C14-supplemented diet at 105 cells g−1 diet for 28 days,then the control diet from day 29 to day 38.The number of C14 cells in the intestine of sea cucumber fed the C14-supplemented diet significantly increased from day 7 to day 28,and decreased from day 29 to day 38.Sea cucumber fed with the diet containing C14 showed a significant increase in trypsin activity and lipase activity from day 21 to day 33 compared with the control.Feeding C14 significantly improved the phagocytic activity and respiratory burst in coelomocytes from day 21 to day 35 and from day 14 to day 38,respectively.In addition,there was an obvious enhancement in lysozyme activity(from day 21 to day 38 or day 33),phenoloxidase activity(from day 21 to day 28)and total nitric oxide synthase activity(from day 14 to day 38)in coelomic fluid supernatant and/or coelomocyte cell lysate supernatant compared with the control.There were significant positive correlations between the number of C14 cells colonizing the intestine and trypsin activity of the intestine,lysozyme activity of the coelomic fluid supernatant and coelomocyte lysate supernatant from sea cucumber.These data suggested that the number of C14 cells should be maintained at 105 cfu(colony-forming units)g−1 intestine material for the maximum benefit. 展开更多
关键词 Apostichopus japonicas feeding duration colonization Metschnikowia sp.C14 digestive enzyme activity immune parameter
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Relationships between plant colonization and soil characteristics in the natural recovery of an earthquaketriggered debris flow gully in the Wanglang National Nature Reserve,China 认领 引用 被引量：2
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作者 XU Bo WANG Jin-niu +1 位作者 SHI Fu-sun WU Ning 《Journal of Mountain Science》 SCIE CSCD 2016年第1期59-68,共10页
Fragmentation and loss of habitats due to natural disasters, like earthquakes and earthquaketriggered debris flows are existing threats to the long- term survival of the giant panda （Ailuropoda melanoleuca）. To bett... Fragmentation and loss of habitats due to natural disasters, like earthquakes and earthquaketriggered debris flows are existing threats to the long- term survival of the giant panda （Ailuropoda melanoleuca）. To better understand natural recovery processes of the damaged habitat, field investigation and laboratory analysis were used to analyze relationships between plant colonization and soil characteristics in an over 3o-year natural recovery of a damaged giant panda habitat in a debris flow gully after the 1976 Songpan-Pingwu earthquake in Sichuan Province, China. Four different damaged sites were selected that located at the center of the gully （center）, on a flat alluvial fan （fan）, in a side slope of the gully （slope）, and at the ecotone between the gully and native forest （ecotone）. Vegetation characteristics, soil physicochemical properties, and microbial biomass in the different sites and soil depths were measured. After the natural recovery, the soil fertility, water retention, and microbial biomass were highest at ecotone, followed by fan, slope, and center. Only a few perennial herbs colonized at center; shrubs started to invade at fan and slope, and the native trees dominated the community of ecotone. Furthermore, Fargesia spathacea （food for the giant panda） started to be re-established at ecotone, and the community characteristic of ecotone recovered similarly to the native habitat. These results suggested that improving the soil fertility, water retaining capacity and microbial biomass is fundamental to the plant colonization, particular for F. spathacea＇s re- establishment in a damaged giant panda habitat. 展开更多
关键词 Plant colonization Soil physicochemicalproperties Soil microbial biomass Natural recovery Giant panda Habitat
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Colonization Characteristics and Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Iris lactea in Songnen Saline-alkaline Grassland 认领 引用 被引量：3
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作者 Chunxue Yang Yajie Liu +1 位作者 Wenna Zhao Na Wang 《Phyton-International Journal of Experimental Botany》 SCIE 2021年第3期719-729,共11页
To understand arbuscular mycorrhizal(AM)fungi resources and develop AM fungal species in ornamental plants with saline-alkaline tolerances,Iris lactea,which grows in the Songnen saline-alkaline grassland with a high o... To understand arbuscular mycorrhizal(AM)fungi resources and develop AM fungal species in ornamental plants with saline-alkaline tolerances,Iris lactea,which grows in the Songnen saline-alkaline grassland with a high ornamental value,was selected as the experimental material,and the colonization characteristics of its roots and the AM fungal diversity in its rhizosphere were explored.The results of the observations and calculations of mycorrhizae from ten different samples showed that AM fungi colonized the roots of I.lactea and formed Arum-type mycorrhizal structures.There was a significant correlation between soil spore density and pH value,while the colonization rate showed a fluctuating trend with increasing pH values.The observed colonization intensities were of Levels II(1%–10%)or III(11%–50%),and the vesicle abundances were of grades A2 or A3 among different sites.AM fungi produced a large number of mycelia and vesicles in the roots of I.lactea after colonization.Thirty-seven species belonging to 15 genera of AM fungi were isolated from the rhizosphere of I.lactea and identified by morphological identification.Funneliformis and Glomus were the dominant genera,accounting for 21.79%and 20.85%of the total number,respectively.F.mosseae and Rhizophagus intraradices were isolated in all samples with importance values of 58.62 and 51.19,respectively.These results are expected to provide a theoretical basis for the analysis of the salt tolerance mechanism of I.lactea and for the discovery,exploration and further screening of AM fungal resources with salinity tolerances in saline-alkaline soils. 展开更多
关键词 Iris lactea colonization characteristics morphology of AM fungal spores saline-alkaline soils
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Biodiversity of arbuscular mycorrhizal colonization and spore population in different agroforestry trees and crop species growing in Dinajpur,Bangladesh 认领 引用 被引量：5
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作者 M. A. U. Mridha P. P. Dhar 《Journal of Forestry Research》 CAS 2007年第2期91-96,共6页
Biodiversity of arbuscular mycorrhizal colonization and spore population was investigated in different agroforestry trees and crop species collected from different locations of Dinajpur district of Bangladesh. Roots a... Biodiversity of arbuscular mycorrhizal colonization and spore population was investigated in different agroforestry trees and crop species collected from different locations of Dinajpur district of Bangladesh. Roots and rhizosphere soils of Albizia procera Benth., Capsicum frutescens L, Curcuma domestica Vahl., Dalbergia sissoo Roxb. and Swietenia macrophylla King. from Dashmail; C. domestica, D. sissoo, Eucalyptus camaldulensis Dehnn., Gmelina arborea （Roxb） DC and Oryza sativa L. from Kantaji and C. domestica, D. sissoo, Litchi chinensis Sonn. and O. sativa from Ramsagar were collected. Roots and soils were assessed following standard methods. The range of AM colonization was recorded 36%-79% from Dashmail. The highest AM colonization was recorded in C. frutescens （79%） and the lowest was in C. domestica （36%）. The range of colonization was recorded as 33%-70% from Kantaji. The highest AM colonization was recorded in G. arborea （70%） and the lowest was in O. sativa （33%）. The range of AM colonization was recorded as 35%-70% from Ramsagar. The highest AM colonization was recorded in D. sissoo （70%） and the lowest was in O. sativa （35%）. Arbuscular mycorrhizal spore population varied from 54 to 140/100g dry soil in the soils from Dashmail. The highest was in the soils of D. sissoo （140） and the lowest was in C. domestica （54）. The spore population varied from 63 to 221 in Kantaji. The highest was in G. arborea （221） and the lowest was in O. sativa （63）. The range population in Ramsagar varied from 69 to 160. The highest was recorded in D. sissoo （160） and the lowest was in L. chinensis （69）. No significant relationship of soil pH and soil OM with AM colonization and with spore population was observed. Simpson＇s index of diversity （Ds） and Shannon＇s index of diversity- （Hs） were highest in the soil of D. sissoo from Kantaji and the lowest in the soils of O. sativa from Ramsagar. Biodiversity of AM colonization, spore population and the distribution of AM fungi in the rhizosphere soils of different agroforestry plants indicated the occurrence of AM fungi, mycotrophic nature of the trees and crop species, contribution and necessity of AM fungi and the AM dependence of the agroforestry plants growing in Dinajpur district of Bangladesh. 展开更多
关键词 Biodiversity Agroforestry Mycorrhiza Colonization Spore population.
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Confocal Laser Scanning Microscope Evaluation of Early Bacterial Colonization on Zirconium Oxide and Titanium Surfaces:An in vivo Study 认领 引用 被引量：1
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作者 王敏 白轶 +3 位作者 YANG Hui ZOU Haixiao 夏海斌 王贻宁 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2013年第2期396-399,共4页
To investigate the bacterial colonization on zirconium oxide and titanium surfaces in vivo quantitatively using a confocal laser scanning microscope （CLSM）. Ten samples of zirconium oxide ceramic and commercially pu... To investigate the bacterial colonization on zirconium oxide and titanium surfaces in vivo quantitatively using a confocal laser scanning microscope （CLSM）. Ten samples of zirconium oxide ceramic and commercially pure titanium were fabricated and polished using silicon carbide abrasive paper. One sample from each group was evaluated topographic pattern under a scanning electron microscope. One sample from each group was to evaluate roughness using a profilometer. Eight volunteers were selected. The samples were cemented at the buccal surfaces of upper first molars. All samples were removed after 48 hours, immersed in SYTO-9 and propidium iodide fluorescent to stain for adherent bacteria and obseIved with CLSM. Fewer bacteria were observed in zirconia group than titanium group. However, there was no statistical difference between two groups. The experimental results demonstrate that zirconium oxide may be considered as a promising material for dental implant abutments. 展开更多
关键词 dental implant abutment zirconium oxide bacterial colonization confocal laser scanning microscope
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Celecoxib inhibits Helicobacter pylori colonization-related factors 认领 引用 被引量：1
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作者 Wang, Jing Wang, Wei-Hong +1 位作者 Li, Jiang Liu, Fang-Xun 《World Journal of Gastroenterology》 SCIE CAS 2010年第7期846-853,共8页
AIM:To investigate the effect of celecoxib,a selective COX-2 inhibitor,on Helicobacter pylori(H.pylori) colonization-related factors and its mechanism.METHODS:After co-incubation with celecoxib,morphology of H.pylori ... AIM:To investigate the effect of celecoxib,a selective COX-2 inhibitor,on Helicobacter pylori(H.pylori) colonization-related factors and its mechanism.METHODS:After co-incubation with celecoxib,morphology of H.pylori strain 26695 was observed under a transmission electron microscope.Flagella motility was assessed by stab agar motility test.Adherence of H.pylori to AGS cells was determined by enzyme linked immunosorbent assay.Levels of mRNA expression in flagellar genes(flaA,flaB),urease genes(ureA,ureB)and adhesin genes(babA,sabA,alpA,alpB,hpaA,hopZ)were measured by real-time polymerase chain reaction.RESULTS:Separation and non-integrity of bacterial cell wall,rarefaction and asymmetry of cytoplasm,and even lysis of H.pylori were observed in the presence of celecoxib.When H.pylori strains were incubated in the presence of celecoxib,their flagellar motility and adherence to AGS cells were inhibited.The expression of ureA,ureB,babA,sabA,alpA,alpB,hpaA,hopZ was up-regulated while the expression of flaA,flaB was down-regulated in the presence of celecoxib.CONCLUSION:Celecoxib inhibits flagellar motility and adherence of H.pylori to AGS cells,and destructs their normal structure in vitro. 展开更多
关键词 Helicobacter pylori Celecoxib Colonization Ultrastructure
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