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Morphological changes and gibberellin-related gene expression patterns during fruit growth and development of Amomum villosum 认领 引用 被引量：1
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作者 LÜ Bingding ZHANG Yuyi +5 位作者 TANG Liyun HU Jiajia SU Jing XU Jie HE Zhuohang HE Guozhen 《中山大学学报（自然科学版）（中英文）》 CAS CSCD 北大核心 2026年第3期19-32,共14页
The fruit of Amomum villosum is highly valued for its medicinal and edible properties.However,the fruit abscission rate is exceptionally high,resulting in low unit yield.To better understand the fruit growth and devel... The fruit of Amomum villosum is highly valued for its medicinal and edible properties.However,the fruit abscission rate is exceptionally high,resulting in low unit yield.To better understand the fruit growth and development,this study investigated the characteristic changes of fruits and the expression patterns of GA-related genes in both fruits and fruit stalks.Results revealed that it takes approximately 90 days for the ovary of A.villosum to reach maturity.Fruit growth pattern exhibited a slow-fast-slow trend,with the peak of fruit weight accumulation occurring between the 14 and 25 days after artificial pollination(DAP).Before the 27 DAP,significant changes were observed in the ovary,fruit color,pericarp thickness,and fruit thorn length.Additionally,ten candidate GA-related genes,including 1 GA13ox,1 GA20ox,1 GA3ox,2 GA2oxs,4 GID1cs,and 1 DELLA,were analyzed using bioinformatics tools.The GA-related genes in fruit and fruit stalk exhibited significant correlations with fruit growth/development and fruit dropping.Overall,the fruit growth and development law,and the expression patterns of GA-related genes provided a theoretical foundation for further functional study and the screening of candidate genes for potential applications in A.villosum. 展开更多
关键词 Amomum villosum gibberellin fruit development gene expression pattern bioinformatic
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Molecular evolutionary analysis and expression patterns of nitrogen assimilation-related gene families in Lycium ruthenicum under salt stress 认领 引用
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作者 Jianwei Qi Jun Zhao +6 位作者 Songsong Lu Qianwen Song Luna Xing Weibo Du Xiaowei Zhang Xiaolei Zhou Yanjun Ma 《Fruit Research》 2025年第1期162-177,共16页
Nitrogen assimilation plays a critical role in the response of plants to salt stress.In this study,bioinformatics and computational biology methods were employed to identify members of the Lycium GS,GOGAT,and GDH gene... Nitrogen assimilation plays a critical role in the response of plants to salt stress.In this study,bioinformatics and computational biology methods were employed to identify members of the Lycium GS,GOGAT,and GDH gene families,and analyzed their expression patterns and transcriptional regulatory networks under salt stress in black wolfberry(Lycium ruthenicum).In addition,the adaptive evolutionary mechanisms of the black wolfberry Fd-GOGAT gene were explored.The results showed that the genomes or transcriptomes of Lycium species contain two to four GS genes,two GOGAT genes,and three to four GDH genes.Following NaCl stress,the expression of Fd-GOGAT and NADH-GOGAT in the leaves of black wolfberry was downregulated with increasing time and NaCl concentration;in the roots,Fd-GOGAT and NADH-GOGAT were upregulated by 1.79-fold and 1.40-fold,respectively,12 h after severe NaCl stress.GS2 and GS1.1 are highly expressed in leaves and roots,respectively,and their expression is significantly upregulated under NaCl stress.The expression of GDH in leaves is downregulated under salt stress,whereas in roots,the expression of NADP-GDH and GDH2 is upregulated under NaCl stress.C2H2-63,a key transcription factor regulating nitrogen assimilation in black wolfberry under salt stress,is likely to promote nitrogen assimilation through its upregulated expression.Additionally,seven positively selected sites(p.Glu220,p.Ala388,p.Ala880,p.Ser921,p.Leu1319,p.Ser1350,and p.Ser1570)were found in the Fd-GOGAT protein of black wolfberry.Compared to Ningxia wolfberry(L.barbarum),these mutation sites enhance the affinity of Fd-GOGAT for its substrate Glu,which helps to increase the catalytic efficiency of Fd-GOGAT in black wolfberry under salt stress.In conclusion,the adaptation of salt stress-related nitrogen assimilation genes in black wolfberry involves both gene expression regulation and adaptive evolution.Enhancing the GS/GOGAT pathway under salt stress is a primary strategic choice for nitrogen assimilation in black wolfberry. 展开更多
关键词 transcriptional regulatory networks computational biology methods salt stress genomes transcriptome molecular evolutionary analysis expression patterns nitrogen assimilation adaptive evolutionary mechanisms
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The age-related expression patterns of Larix kaempferi AP2 subfamily genes and functional dissection of LkTOE1-2 in seed formation and germination 认领 引用
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作者 Zha-Long Ye Xiang-Yi Li +2 位作者 Man-Li Nong Xiao-Mei Sun Wanfeng Li 《Forestry Research》 2025年第1期50-58,共9页
Conifers pose challenges for breeding programs due to their extended vegetative phases.Despite the critical role of APETALA2(AP2)in regulating phase transitions,the AP2/ERF superfamily remains largely unexplored in gy... Conifers pose challenges for breeding programs due to their extended vegetative phases.Despite the critical role of APETALA2(AP2)in regulating phase transitions,the AP2/ERF superfamily remains largely unexplored in gymnosperms.Here,the first genome-wide analysis of the AP2/ERF superfamily in a conifer,Larix kaempferi(Japanese larch)is presented,and 374 members were identified.Among all eight paralogs,four euAP2 lineage genes,TARGET OF EATs(TOEs),exhibit age-decreased expression patterns.Functional characterization of LkTOE1-2 demonstrates its involvement in somatic embryogenesis and seed germination.Importantly,the RUBY reporter system confirmed an enhanced promoter activity in somatic embryo maturation.Over-expression of LkTOE1-2 in Arabidopsis accelerates seed germination by 23.77%,bolting by 6.93%,and flowering by 5.92%.This study provides not only an expanded genomic resource for gymnosperms but also a candidate gene(LkTOE1-2)to shorten the juvenile phase of larch via molecular breeding. 展开更多
关键词 molecular breeding coniferlarix kaempferi japanese euap lineage genestarget age related expression patterns ap erf superfamily somatic embryogenesis juvenile phase seed germination
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Assignment and expression patterns of porcine muscle-specific isoform of phosphoglycerate mutase gene 认领 引用 被引量：6
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作者 Haifang Qiu Shuhong Zhao +2 位作者 Xuewen Xu Martine Yerle Bang Liu 《Journal of Genetics and Genomics》 SCIE CAS 北大核心 2008年第5期257-260,共4页
It has been reported that the muscle-specific isoform （type M, PGAM2） of phosphoglycerate mutase （PGAM） is a housekeeping enzyme; it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the gl... It has been reported that the muscle-specific isoform （type M, PGAM2） of phosphoglycerate mutase （PGAM） is a housekeeping enzyme; it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In this study, the cDNA of the porcine Pgam2 was cloned. This gene contains an open reading frame of 765 bp encoding a protein of 253 residues, and the predicted protein sequences share high similarity with other mammalians, 96% identity with humans, and 94% identity with mouse and rats. Pgam2 was mapped to SSC18q13-q21 by the RH panel. In this region, there are several QTLs, such as fat ratio, lean percentage, and diameter of muslce fiber, which affect meat production and quality. The reverse transcriptase-polymerase chain reaction revealed that the porcine Pgam2 gene was mainly expressed in the muscle tissue （skeletal muscle and cardiac muscle）, and was expressed highly at skeletal muscle development stages （embryonic periods： 33, 65, and 90 days post-conception （dpo）; postnatal pigs： 4 days and adult）. This indicates that the Pgam2 gene plays an important role in muscle growth and development. In addition, it was demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei. 展开更多
关键词 assignment expression patterns subcellular localization porcine pgma2
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Genome-Wide Analysis and Expression Patterns of the YUCCA Genes in Maize 认领 引用 被引量：11
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作者 Wenlan Li Xiangyu Zhao Xiansheng Zhang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2015年第12期707-710,共4页
Auxin plays important roles in various aspects of plant growth and development （Zhao, 2010）. In Arabidopsis, a number of YUCCA （YUC） genes, which are involved in auxin biosyn- thesis, have been identified （Zhao e... Auxin plays important roles in various aspects of plant growth and development （Zhao, 2010）. In Arabidopsis, a number of YUCCA （YUC） genes, which are involved in auxin biosyn- thesis, have been identified （Zhao et al., 2001; Woodward et al., 2005; Cheng et al., 2006, 2007; Kim et al., 2007; Chen et al., 2014）. YUC genes encode flavin monooxygenases （FMOs） that convert indole-3-pyruvate （IPA） to indole-3-acetic acid （IAA） （Zhao, 2012）. The Arabidopsis YUC family is comprised of 11 members （Zhao et al., 2001; 展开更多
关键词 Genome-Wide Analysis and Expression Patterns of the YUCCA Genes in Maize SPI
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Transcriptomic analyses to summarize gene expression patterns that occur during leaf initiation of Chinese cabbage 认领 引用 被引量：1
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作者 XiaoXue Sun Zihan Liu +4 位作者 Rui Liu Johan Bucher Jianjun Zhao Richard G.F.Visser Guusje Bonnema 《Horticulture Research》 SCIE CSCD 2024年第4期251-260,共10页
In Chinese cabbage,rosette leaves expose their adaxial side to the light converting light energy into chemical energy,acting as a source for the growth of the leafy head.In the leafy head,the outer heading leaves expo... In Chinese cabbage,rosette leaves expose their adaxial side to the light converting light energy into chemical energy,acting as a source for the growth of the leafy head.In the leafy head,the outer heading leaves expose their abaxial side to the light while the inner leaves are shielded from the light and have become a sink organ of the growing Chinese cabbage plant.Interestingly,variation in several ad/abaxial polarity genes is associated with the typical leafy head morphotype.The initiation of leaf primordia and the establishment of leaf ad/abaxial polarity are essential steps in the initiation of marginal meristem activity leading to leaf formation.Understanding the molecular genetic mechanisms of leaf primordia formation,polar differentiation,and leaf expansion is thus relevant to understand leafy head formation.As Brassica’s are mesa-hexaploids,many genes have multiple paralogues,complicating analysis of the genetic regulation of leaf development.In this study,we used laser dissection of Chinese cabbage leaf primordia and the shoot apical meristem(SAM)to compare gene expression profiles between both adaxial and abaxial sides and the SAM aiming to capture transcriptome changes underlying leaf primordia development.We highlight genes with roles in hormone pathways and transcription factors.We also assessed gene expression gradients along expanded leaf blades from the same plants to analyze regulatory links between SAM,leaf primordia and the expanding rosette leaf.The catalogue of differentially expressed genes provides insights in gene expression patterns involved in leaf development and form a starting point to unravel leafy head formation. 展开更多
关键词 leaf primordi leafy headthe leafy head leafy headin transcriptomic analyses Chinese cabbage gene expression patterns sink organ
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Detection of Cytokine Expression Patterns in the Peripheral Blood of Patients with Acute Leukemia by Antibody Microarray Analysis 认领 引用 被引量：1
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作者 李青 李梅 +4 位作者 吴耀辉 朱晓健 曾辰 邹萍 陈智超 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2014年第2期176-180,共5页
The cytokines of acute leukemia （AL） patients have certain expression patterns, forming a complex network involved in diagnosis, progression, and prognosis. We collected the serum of different AL patients before and... The cytokines of acute leukemia （AL） patients have certain expression patterns, forming a complex network involved in diagnosis, progression, and prognosis. We collected the serum of different AL patients before and after complete remission （CR） for detection of cytokines by using an antibody chip. The expression patterns of cytokines were determined by using bioinformatics computational analysis. The results showed that there were significant differences in the cytokine expression patterns between AL patients and normal controls, as well as between acute myeloid leukemia （AML） and acute lymphoblastic leukemia （ALL）. In confirmatory test, ELISA revealed the expression of uPAR in AL. Moreover, the bioinformatic analysis showed that the differentially expressed cytokines among the AL groups were involved in different biological behaviors and were closely related with the development of the disease. It was concluded that the cytokine expression pattern of AL patients is significantly different from that of healthy volunteers. Also, differences of cytokine expression patterns exist between AML and ALL, and between before and after CR in the same subtype of AL, which holds important clinical significance for revealing disease progression. 展开更多
关键词 cytokine microarray acute leukemia expression patterns bioinformatics
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Identification of the Calcineurin B-like gene family and gene expression patterns in response to low temperature stress in Prunus mume 认领 引用 被引量：1
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作者 Haolin Liu Lihong Hao +6 位作者 Xuesi Zhang Yetong Zhang Hongru Wang Jingcan Wang Ziguang Liu Qin Zhang Ping Li 《Tropical Plants》 2024年第1期370-382,共13页
The CBL gene family is an important family in the Ca2+mediated signal transduction pathway in plants and plays a crucial role in plant stress responses and growth development.However,research on the response of mem... The CBL gene family is an important family in the Ca2+mediated signal transduction pathway in plants and plays a crucial role in plant stress responses and growth development.However,research on the response of members of the Prunus mume CBL gene family to low temperature stress remains scarce.In this study,we systematically analyzed the protein physicochemical properties,chromosome localization,phylogenetic evolution,gene structure,conserved domains,cis-acting elements,and gene expression patterns in response to low temperature stress of members of the P.mume CBL gene family using bioinformatics tools.Six PmCBL gene family members were identified in the P.mume genome.Phylogenetic trees were constructed,revealing three subfamilies named Group I,Group II,and Group III.In the P.mume gene family,PmCBL4 and PmCBL5 were paralogous genes.The members of the P.mume CBL gene family were unevenly distributed on three chromosomes.The CBL encoding protein,the number of isoelectric points(pI),the number of introns and exons of the six gene families were different.Analysis of the upstream 700 bp promoter sequences of the P.mume CBL gene family revealed the presence of various types of cis-acting elements involved in non-biological stress responses.Among the six identified genes,each gene exhibited different expression patterns in response to low temperature.Among them,the up-regulated expression of PmCBL5 was the largest,and the expression of PmCBL1,PmCBL3 and PmCBL5 showed the up-regulated trend.These results indicated that PmCBL1,PmCBL3,PmCBL5,and PmCBL6 were key genes involved in the response of P.mume to low temperature stress.This study provided comprehensive and systematic analysis of the P.mume CBL gene family members and identified key genes involved in the response to low temperature stress,thereby providing genetic resources for molecular breeding programs aimed at enhancing cold resistance in P.mume. 展开更多
关键词 prunus mume CBL gene family calcineurin B protein gene expression patterns low temperature stress cbl gene signal transduction pathway protein physicochemical propertieschromosome localizationphylogenetic
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Expression Patterns of Inducible Cre Recombinase Driven by Differential Astrocyte-Specific Promoters in Transgenic Mouse Lines 认领 引用 被引量：8
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作者 Neng-Yuan Hu Ya-Ting Chen +9 位作者 Qian Wang Wei Jie Yi-Si Liu Qiang-Long You Ze-Lin Li Xiao-Wen Li Sophie Reibel Frank W.Pfrieger Jian-Ming Yang Tian-Ming Gao 《Neuroscience Bulletin》 SCIE CAS CSCD 2020年第5期530-544,共15页
Astrocytes are the most abundant cell type in the central nervous system(CNS).They provide trophic support for neurons,modulate synaptic transmission and plasticity,and contribute to neuronal dysfunction.Many transgen... Astrocytes are the most abundant cell type in the central nervous system(CNS).They provide trophic support for neurons,modulate synaptic transmission and plasticity,and contribute to neuronal dysfunction.Many transgenic mouse lines have been generated to obtain astrocyte-specific expression of inducible Cre recombinase for functional studies;however,the expression patterns of inducible Cre recombinase in these lines have not been systematically characterized.We generated a new astrocyte-specific Aldh1 l1-CreERT2knock-in mouse line and compared the expression pattern of Cre recombinase between this and five widely-used transgenic lines(hGfap-CreERT2from The Jackson Laboratory and The Mutant Mouse Resource and Research Center,Glast-CreERT2,Cx30-CreERT2,and Fgfr3-iCreERT2)by crossing with Ai14 mice,which express tdTomato fluorescence following Cre-mediated recombination.In adult Aldh1 l1-CreERT2:Ai 14 transgenic mice,tdTomato was detected throughout the CNS,and five novel morphologicallydefined types of astrocyte were described.Among the six evaluated lines,the specificity of Cre-mediated recombination was highest when driven by Aldh1 l1 and lowest when driven by hGfap;in the latter mice,co-staining between tdTomato and NeuN was observed in the hippocampus and cortex.Notably,evident leakage was noted in Fgfr3-iCreERT2mice,and the expression level of tdTomato was low in the thalamus when Cre recombinase expression was driven by Glast and in the capsular part of the central amygdaloid nucleus when driven by Cx30.Furthermore,tdTomato was clearly expressed in peripheral organs in four of the lines.Our results emphasize that the astrocyte-specific CreERT2transgenic lines used in functional studies should be carefully selected. 展开更多
关键词 Astrocytes Cre recombinase Expression pattern Aldh111 Morphology
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Preliminary analysis of two NAC transcription factor expression patterns in Larix olgensis 认领 引用
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作者 Qing Cao Peiqi An +3 位作者 Sufang Zhang Junhui Wang Hanguo Zhang Lei Zhang 《Journal of Forestry Research》 SCIE CAS CSCD 2022年第2期601-609,共9页
The NAC transcription factor family is plant-specific with various biological functions.However,there are few studies on the NAC gene involving coniferous species.Bioinformatics research and expression analysis of NAC... The NAC transcription factor family is plant-specific with various biological functions.However,there are few studies on the NAC gene involving coniferous species.Bioinformatics research and expression analysis of NAC genes in Larix olgensis can be used to analyse the function of the NAC gene in the future.Screening of excellent genetic materials and molecular breeding have been utilized to cultivate high-quality,stress-resistant larches.According to the transcriptome data for L.olgensis,the genes Uni-gene81490 and Unigene70699 with complete ORFs(open reading frames)were obtained by conserved domain analy-sis and named LoNAC1 and LoNAC2,respectively.The cDNAs of LoNAC1 and LoNAC2 were 1971 bp and 1095 bp in length,encoding 656 and 364 amino acids,respectively.The molecular weights of the proteins encoded by the two genes were predicted to be 72.61 kDa and 41.13 kDa,and subcellular localization analysis indicated that the proteins were concentrated in the nucleus.The results of real-time quantitative PCR analysis showed that at different growth stages and in different tissues of L.olgensis,the relative expression levels of the two NAC genes were highest in the stem,and the expression differences were more obvious in non-lignified tissues.After drought,salt and alkali stress and hormone treatment,expression was induced to different degrees.The expression levels of LoNAC1 and LoNAC2 in semi-lignified L.olgensis were higher than in the other two periods(non-lignified and lignified),and expression levels significantly increased under drought and salt stress.Relative expression levels changed under hormone treatment.It is speculated that these two genes may not only be related to drought and salt stress and secondary growth but may also be induced by hormones such as abscisic acid.Overall,LoNAC1 and LoNAC2 are genetic materials that can be used for molecular breeding of larch. 展开更多
关键词 Larix olgensis NAC transcription factor Bioinformatics analysis Expression patterns
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Molecular characterization and developmental expression patterns of thyroid hormone receptors(TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata 认领 引用 被引量：4
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作者 Qinqin Lou Yinfeng Zhang +4 位作者 Dongkai Ren Haiming Xu Yaxian Zhao Zhanfen Qin Wuji Wei 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2014年第10期2084-2094,共11页
Considering some advantages of Rana nigromaculata as an experimental species, we propose that this species, like Xenopus laevis, could be used to assay thyroid hormone（TH） signaling disrupting actions. To validate t... Considering some advantages of Rana nigromaculata as an experimental species, we propose that this species, like Xenopus laevis, could be used to assay thyroid hormone（TH） signaling disrupting actions. To validate the utilizability of R. nigromaculata, we investigated the responsiveness of R. nigromaculata to a TH receptor（TR） agonist（T3） and antagonist（amiodarone） by analyzing expression, based on characterizing TR cDNA and developmental expression patterns. With high levels of identity with the corresponding genes in X. laevis, both TRα and TRβ in R. nigromaculata exhibited roughly similar developmental expression patterns to those of X. laevis, in spite of some species-specific differences. Both TRα and TRβ expression had greater changes in the liver and intestine than in the tail and brain during metamorphosis. T3 exposure for 2 days induced more dramatic increases of TRβ expression in stage 27 than in stage34 tadpoles but not in stage 42 tadpoles, showing that the responsiveness of R. nigromaculata to TH decreased with development and disappeared at the onset of metamorphic climax.Corresponding to greater changes of TRβ expression in the liver and intestine than in the tail and brain during metamorphosis, the liver and intestine had higher responsiveness to exogenous T3 than the tail and brain. Amiodarone inhibited T3-induced TRβ expression. Our results show that R. nigromaculata can be used as a model species for assaying TH signaling disrupting actions by analyzing TRβ expression, and intestine tissues at stage 27 are ideal test materials due to high responsiveness and easy accessibility. 展开更多
关键词 Rana nigromaculata Thyroid hormone receptor Thyroid hormone signaling Developmental expression pattern Responsiveness
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Genome-wide identification and characterization of the JAZ gene family and its expression patterns under various abiotic stresses in Sorghum bicolor 认领 引用 被引量：5
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作者 DU Qiao-li FANG Yuan-peng +3 位作者 JIANG Jun-mei CHEN Mei-qing LI Xiang-yang XIE Xin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2022年第12期3540-3555,共16页
The jasmonate ZIM domain(JAZ)protein belongs to the TIFY((TIF[F/Y]XG)domain protein)family,which is composed of several plant-specific proteins that play important roles in plant growth,development,and defense respons... The jasmonate ZIM domain(JAZ)protein belongs to the TIFY((TIF[F/Y]XG)domain protein)family,which is composed of several plant-specific proteins that play important roles in plant growth,development,and defense responses.However,the mechanism of the sorghum JAZ family in response to abiotic stress remains unclear.In the present study,a total of 17 JAZ genes were identified in sorghum using a Hidden Markov Model search.In addition,real-time quantification polymerase chain reaction(RT-qPCR)was used to analyze the gene expression patterns under abiotic stress.Based on phylogenetic tree analysis,the sorghum JAZ proteins were mainly divided into nine subfamilies.A promoter analysis revealed that the SbJAZ family contains diverse types of promoter cis-acting elements,indicating that JAZ proteins function in multiple pathways upon stress stimulation in plants.According to RT-qPCR,SbJAZ gene expression is tissuespecific.Additionally,under cold,hot,polyethylene glycol,jasmonic acid,abscisic acid,and gibberellin treatments,the expression patterns of SbJAZ genes were distinctly different,indicating that the expression of SbJAZ genes may be coordinated with different stresses.Furthermore,the overexpression of SbJAZ1 in Escherichia coli was found to promote the growth of recombinant cells under abiotic stresses,such as PEG 6000,NaCl,and 40℃ treatments.Altogether,our findings help us to better understand the potential molecular mechanisms of the SbJAZ family in sorghum in response to abiotic stresses. 展开更多
关键词 Sorghum bicolor gene family identification JAZ family abiotic stress expression pattern
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Expression patterns of OCT4, NANOG, and SOX2 in goat preimplantation embryos from in vivo and in vitro 认领 引用 被引量：2
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作者 YU Xiao-li ZHAO Xiao-e +1 位作者 WANG Hua-yan MA Bao-hua 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第7期1398-1406,共9页
The transcription factors, including OCT4, NANOG, and SOX2, played crucial roles in the maintenance of self-renewal and pluripotency in embryonic stem cells （ESCs）. They expressed in preimplantation mammalian develo... The transcription factors, including OCT4, NANOG, and SOX2, played crucial roles in the maintenance of self-renewal and pluripotency in embryonic stem cells （ESCs）. They expressed in preimplantation mammalian development with spa- tio-temporal pattern and took part in regulation of development. However, their expression and roles in goat had not been reported. In the present study, the expression of OCT4, NANOG, and SOX2 in goat preimplantation embryos both in vivo and in vitro were detected by real-time RCR and immunofluorescence. For in vivo fertilized embryos, the transcripts of OCT4, NANOG, and SOX2 could be detected from oocytes to blastocyst stage, their expression in morula and blastocyst stages was much higher than other stage. OCT4 protein was detected from oocyte to blastocyst, but the fluorescence was more located-intensive with nuclei from 8-cell stage, its expression present in both inner cell mass （ICM） and trophoblast cells （TE） at blastocyse stage. NANOG protein was similar to OCT4, the signaling of fluorescence completely focused on cell nuclei, while the SOX2 firstly showed nuclei location in morula. Comparing to in vivo fertilized embryo, the mRNA of these three transcription factors could be detected at 8-cell stage in parthenogenetic embryos （in vitro）. Thereafter, the expressional level rose gradually along with embryo development. The locations of OCT4 and NANOG proteins were similar to in vivo fertilized embryos, and they located in cell nuclei from morula to blastocyst stage, while SOX2 protein firstly could be detected in cell nuclei at 8-cell stage. These differences suggested that OCT4, NANOG, and SOX2 played different function in regulating development of goat preimplantation embryos. These results may provide a novel insight to goat embryo development and be useful for goat ESCs isolation. 展开更多
关键词 goat OCT4 NANOG SOX2 embryo expression pattern
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Temporal and Spatial Expression Patterns of Sox1 Gene in Xenopus laevis Embryo 认领 引用 被引量：4
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作者 马莉 赵树华 +1 位作者 孔清华 毛炳宇 《Zoological Research》 北大核心 2007年第4期403-408,共6页
We describe the temporal and spatial expression pattern of Sox 1 gene during Xenopus laevis early development and compare the expression patterns of Sox 1-3 in the developing eye and brain. Alignment of Sox 1-3 amino ... We describe the temporal and spatial expression pattern of Sox 1 gene during Xenopus laevis early development and compare the expression patterns of Sox 1-3 in the developing eye and brain. Alignment of Sox 1-3 amino acid sequences shows a high conservation within the HMG-box DNA binding domains. RT-PCR analysis indicates that Sox 1 is expressed throughout development from the unfertilized egg to at least the tadpole stage, although at different expression levels. The transcripts of XSox 1 are detected in the animal pole at cleavage and blastrula stages and mainly in the central nervous system （CNS） and the developing eye at neurula stages. The study of the developmental expression of XSox 1 will aid in the elucidation of the function of SoxB 1 subgroup genes in vertebrate neurogenesis. 展开更多
关键词 Sox 1 Sox2 Sox3 Xenopus laevis Expression pattern
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Wheat Lysin-Motif-Containing Proteins Characterization and Gene Expression Patterns under Abiotic and Biotic Stress 认领 引用
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作者 Minjie Liu Na Gao +2 位作者 Yaqiong Zhao Yingpeng Wu Zongying Yuan 《Phyton-International Journal of Experimental Botany》 SCIE 2022年第11期2367-2382,共16页
Lysin motif(LysM)-containing proteins(LYPs)are important pattern recognition receptors in plants.However,the evolutionary history and characteristics of LYP genes remain largely unclear in wheat.In this study,62 LYPs ... Lysin motif(LysM)-containing proteins(LYPs)are important pattern recognition receptors in plants.However,the evolutionary history and characteristics of LYP genes remain largely unclear in wheat.In this study,62 LYPs were identified at genome wide in wheat.Based on phylogenetic and domain analysis,wheat LYPs were classified into 6 subgroups(group LysMe,LysMn,LYP,LYK,LysMFbox).Syntenic analysis showed the evolution of LYP genes in wheat.RNA-seq data showed that 22 genes were not expressed at any tissue or stress stimulation period.Some LYP and LYK genes were tissue-or stage-specific.The majority of TaLYK5s,TaLYK6s,TaLYP2s and TaLysMns genes were induced under chitin,flg22 and fungal treatment.qRT-PCR analysis showed that 4 genes were upregulated during Puccinia triticina infection with a peak at 18 h post inoculation.Our findings suggested that wheat LYPs may have specific roles in response to fungal infection and provided insights into the function and characteristics of wheat LYP genes. 展开更多
关键词 Wheat lysin motif containing protein evolution expression pattern
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Expression Patterns and Functions of Cardiac Pigment Epithelium-Derived Factor During Cardiac Development 认领 引用
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作者 Xing-hui Li Yan-di Wu +10 位作者 Tong-sheng Huang Teng Wu Xin-lu Fu Jiang Qian Yan Zou Cong-hui Shen Shi-jie Xiong Zi-qi Feng Hui-ting Zheng Yuan-jun Ji Wei-bin Cai 《Cardiovascular Innovations and Applications》 2023年第1期10-20,共11页
Objective:This study describes the expression profiles and roles of cardiac pigment epithelium-derived factor(PEDF)during cardiac development.Methods:Gene datasets from the Gene Expression Omnibus(GEO)database were us... Objective:This study describes the expression profiles and roles of cardiac pigment epithelium-derived factor(PEDF)during cardiac development.Methods:Gene datasets from the Gene Expression Omnibus(GEO)database were used to analyze the correlation between cardiac PEDF expression and heart disease.Western blotting,immunohistochemistry,histological staining and echocardiography were used to assess the expression patterns and functions of PEDF during cardiac development.Results:Analysis of GEO data sets indicated that the expression of cardiac PEDF correlated with the occurrence and development of various heart diseases.Western blotting of various tissues in mice at 30 postnatal days of age indicated higher PEDF expression in the heart and aorta than the liver.Immunohistochemical results demonstrated that the ex-pression of cardiac PEDF significantly decreased after birth,mainly because of a significant decrease in PEDF expres-sion in the cytoplasm.Histological staining and echocardiography indicated that PEDF deficiency had no significant effects on cardiac structure,cardiac function and vascular hemodynamics in 8-week-old mice.Conclusion:Cardiac PEDF shows high expression and dynamic changes during cardiac development,but has no effects on cardiac structure,function and vascular hemodynamics. 展开更多
关键词 Expression pattern Pigment epithelium-derived factor Cardiac development
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Pan-genome-wide identification and co-expression analysis of rapeseed SBT genes reveals that BnaSBT1.4 negatively regulates the salt-stress response 认领 引用
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作者 Shengting Li Xiaodong Li +8 位作者 Boyu Meng Daifei Song Yuanyi Mao Lin Liu Wei Chang Yonghai Fan Mingchao Qian Shahid Ullah Khan Kun Lu 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第5期1245-1249,共5页
Subtilases(SBTs)play important roles in plant development and resistance to environmental stresses.However,a systematic pan-genome characterization and functional analysis of the SBT family in polyploid crops had not ... Subtilases(SBTs)play important roles in plant development and resistance to environmental stresses.However,a systematic pan-genome characterization and functional analysis of the SBT family in polyploid crops had not been performed.In this study,we performed a pan-genome analysis of 17 rapeseed genomes and identified 1844 SBT family members,which were divided into 82 orthologous gene groups(OGGs)and classified into six subfamilies.Analysis of gene duplication analysis and gene expression patterns reveled that SBT subfamily 1A contains many core OGGs.The expression levels of the subfamily 1 OGG member Bna SBT.CR04 increased under salt-stress treatment. 展开更多
关键词 gene duplication analysis rapeseed genomes rapeseed sbt genes salt stress response gene expression patterns co expression analysis pan genome analysis orthologous gene groups oggs
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Expression level and spatial distribution of opsin gene in Oratosquilla oratoria compound eye 认领 引用
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作者 Fangrui LOU Xiaowen DUAN +2 位作者 Jiaoli ZHOU Yue DING Zhiqiang HAN 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2026年第1期340-356,共17页
Due to the unique microstructure and diverse opsin genes of the trinocular compound eye,stomatopoda possess an extraordinary ability to perceive multiple properties of light.They not only can detect natural light(NL)a... Due to the unique microstructure and diverse opsin genes of the trinocular compound eye,stomatopoda possess an extraordinary ability to perceive multiple properties of light.They not only can detect natural light(NL)and linearly polarized light(LPL),but also are the only animals capable of recognizing circularly polarized light(CPL).Here,we integrated single-cell RNA sequencing,previously published Illumina data,and in-situ hybridization(ISH)to quantify and localize functional opsin genes in Oratosquilla oratoria,a common stomatopoda species in the China Sea.A total of high-quality 31777 cells were captured for the first time in the O.oratoria compound eye,which were classified into 25 cell subpopulations,and hypothesized that cluster 22 is a critical cell subpopulation responsible for light(whether NL,LPL,or CPL)response in O.oratoria.Furthermore,we propose that the long-wavelengthsensitive opsin gene(lws)gene family,retinol dehydrogenase(rdh),voltage-gated ion channel(vgic),arrestin(arr),and myosin(myo)collectively mediate the light response in O.oratoria.Considering that very few vision-related opsin genes show differential expression in right-handed CPL(RCPL)-vs.-dark(DL),which provides additional evidence that stomatopoda cannot recognize RCPL.Meanwhile,we believe that UV-stimulated scaffold protein A(uvssa)and red pigment concentrating hormone(rpch)play special contributions in the left-handed CPL(LCPL)environment response.ISH revealing that 16 lws,6 middle-wavelength-sensitive(mws),and 2 ultraviolet(uv)opsin genes were expressed in the photoreceptors of the O.oratoria compound eye.Although the inability to determine the functional types of cell subpopulations limits the resolution of opsin genes,these findings systematically elucidate the specific expression patterns of opsin genes in O.oratoria and represent a significant step toward refining the visual ecological theory of O.oratoria and other stomatopod species. 展开更多
关键词 Oratosquilla oratoria opsin gene expression pattern single-cell RNA sequencing in-situ hybridization
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Genome-wide characterization and expression analysis of the cultivated peanut AhPR10 gene family mediating resistance to Aspergillus flavus 认领 引用
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作者 Qi Zhao Mengjie Cui +11 位作者 Tengda Guo Lei Shi Feiyan Qi Ziqi Sun Pei Du Hua Liu Yu Zhang Zheng Zheng Bingyan Huang Wenzhao Dong Suoyi Han Xinyou Zhang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第1期56-67,共12页
The pathogenesis-related protein PR10 plays a vital role in plant growth,development,and stress responses.This study systematically identified and analyzed PR10 genes in cultivated peanut(Arachis hypogaea L.),examinin... The pathogenesis-related protein PR10 plays a vital role in plant growth,development,and stress responses.This study systematically identified and analyzed PR10 genes in cultivated peanut(Arachis hypogaea L.),examining their phylogenetic relationships,conserved motifs,gene structures,and syntenic relationships.The analysis identified 54 Ah PR10 genes,which were classified into eight groups based on phylogenetic relationships,supported by gene structure and conserved motif characterization.Analysis of chromosomal distribution and synteny demonstrated that segmental duplications played a crucial role in the expansion of the Ah PR10 gene family.The identified Ah PR10 genes exhibited both constitutive and inducible expression patterns.Significantly,Ah PR10-7,Ah PR10-33,and Ah PR10-41 demonstrated potential importance in peanut resistance to Aspergillus flavus.In vitro fungistatic experiments demonstrated that recombinant Ah PR10-33 effectively inhibited A.flavus mycelial growth.These findings provide valuable insights for future investigations into Ah PR10 functions in protecting peanut from A.flavus infection. 展开更多
关键词 cultivated peanut PR10 phylogenetic analysis expression pattern Aspergillus avus
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Expression Analysis and Transcription Factor Prediction of Fibroblast Growth Factor 10 During Differentiation of Rat L6 Myoblasts 认领 引用
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作者 Shuying FU Yifei HUANG +7 位作者 Gaojian LI Changseng LI Zhiyin DONG Zilan YANG Qiuyu SONG Jingwen ZENG Yu MEN Meiying FENG 《Asian Agricultural Research》 2026年第3期34-38,44,共5页
[Objectives]To characterize the expression pattern of Fibroblast growth factor 10(FGF10)during the differentiation of rat L6 myoblasts and to identify potential key transcription factors(TFs)regulating its expression ... [Objectives]To characterize the expression pattern of Fibroblast growth factor 10(FGF10)during the differentiation of rat L6 myoblasts and to identify potential key transcription factors(TFs)regulating its expression through bioinformatics approaches.[Methods]Rat L6 myoblasts were induced to differentiate by culturing them in DMEM supplemented with 2%donor horse serum(DHS).Morphological changes were observed using an inverted microscope.Cell samples were collected prior to induction(day 0)and on days 1,3,5,and 7 post-induction.The relative expression levels of FGF10 mRNA and protein at each time point were quantified using RT-qPCR and Western blot analysis,respectively.Furthermore,a 2000 bp sequence upstream of the transcription start site of the rat Fgf10 gene was extracted as the promoter region.Putative TF binding sites were predicted using four databases(TRANSFAC,JASPAR,HOCOMOCO,and CISBP),and high-confidence candidates were screened to construct a regulatory network.[Results]Morphological observations confirmed successful differentiation,as evidenced by the appearance of binucleated myotubes on day 3 and the formation of numerous thick,multinucleated myotubes by day 7.Both RT-qPCR and Western blot analysis demonstrated a significant dynamic expression pattern of FGF10.Expression levels were markedly upregulated during the early phase(days 1-3),reaching a peak on day 3(P<0.01),followed by a decline to basal levels during the late phase(days 5-7).Cross-validation across multiple databases identified 48 high-confidence TFs,among which Elf5,Tcf3,Nkx3-2,Zic2,Tcf7,and Egr1 were consistently predicted by all four databases.[Conclusions]FGF10 exhibits high expression levels during the early stage of differentiation,indicating its crucial role in the initiation of myogenesis.The six identified TFs serve as core candidate regulators of Fgf10 expression,offering novel insights into the molecular mechanisms underlying muscle development. 展开更多
关键词 L6 myoblast Differentiation FGF10 Transcription factor Expression pattern
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