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Antitumor effects of different Ganoderma lucidum spore powder in cell-and zebrafish-based bioassays 认领 引用 被引量：27
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作者 Yue-jiao Shi Hua-xian Zheng +4 位作者 Zhu-ping Hong Han-bo Wang Ying Wang Ming-yan Li Zhen-hao Li 《Journal of Integrative Medicine》 SCIE CAS CSCD 2021年第2期177-184,共8页
Objective:Ganoderma lucidum spore(GLS)is gaining recognition as a medicinal part of G.lucidum and has been reported to possess various pharmacological properties,such as antitumor activity.In this work,wall-broken GLS... Objective:Ganoderma lucidum spore(GLS)is gaining recognition as a medicinal part of G.lucidum and has been reported to possess various pharmacological properties,such as antitumor activity.In this work,wall-broken GLS powder(BGLSP)and wall-removed GLS powder(RGLSP),two kinds of GLS powder with different manufacturing techniques,were compared in terms of contents of active constituents and in vivo and in vitro antitumor effects.Methods:The ultraviolet and visible spectrophotometry method was used to determine the contents of polysaccharides and total triterpenoids in BGLSP and RGLSP.Seventeen individual triterpenoids were further quantified using ultra-high-performance liquid chromatography and quantitative analysis of multicomponents by single marker.The antitumor effects of BGLSP and RGLSP were evaluated using in vitro cell viability assay against human gastric carcinoma SGC-7901,lung carcinoma A549 and lymphoma Ramos and further validated by in vivo zebrafish xenograft models with transplanted SGC-7901,A549 and Ramos,Results:The results showed that the contents of polysaccharides,total triterpenoids and individual triterpenoids of RGLSP were significantly higher than those of BGLSP.Although both BGLSP and RGLSP inhibited the three tumor cell lines in vitro in a dose-dependent manner,the inhibitory effects of RGLSP were much better than those of BGLSP.In the in vivo zebra fish assay,RGLSP exhibited more potent inhibitory activities against tumors transpla nted into the zebra fish compared with BGLSP,and the inhibition rates of RGLSP reached approximately 78%,31%and 83%on SGC-7901,A549 and Ramos,respectively.Conclusion:The results indicated that the antitumor effects of GLS were positively correlated with the contents of the polysaccha rides and triterpenoids and demonstrated that the wall-removing manufacturing technique could significantly improve the levels of active constituents,and thereby enhance the antitumor activity. 展开更多
关键词 Ganoderma lucidum Tumor Cancer cell Zebrafish Wall-broken Ganoderma lucidum spore powder Wall-removed Ganoderma lucidum spore powder
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Light repair effect of polysaccharides component from white Ganoderma lucidum and Laminaria japonica fermentation broth attenuates epidermal barrier dysfunction via TRPV4-Keap-1/Nrf2 pathway 认领 引用
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作者 Xinrui Jiang Qianru Sun +6 位作者 Yunxia Chen Ning Su Jiaxuan Fang Zixin Song Meng Li Changtao Wang Dongdong Wang 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期1820-1833,共14页
Epidermal photoinflammation has emerged as an atypical pathological response that poses a growing global threat to human health and well-being.In this study,a polysaccharide component(named LJGp)was isolated from Lami... Epidermal photoinflammation has emerged as an atypical pathological response that poses a growing global threat to human health and well-being.In this study,a polysaccharide component(named LJGp)was isolated from Laminaria japonica and white Ganoderma lucidum fermentation broth,and its reparative effects on UVB-induced epidermal damage were investigated.LJGp was found to restore the migration ability of HaCaT cells,increase antioxidant enzyme activities,and clear excessive intracellular ROS.At 1500μg/mL,ROS levels were reduced to about 50%of those in the UVB group,while IL-6 expression decreased to a level comparable with the control group.At the same time,LJGp increased barrier functional proteins,inhibited desmosome degradation,and maintained the stability of the epidermal barrier.Mechanistic results further showed that LJGp regulated the TRPV4-Keap-1/Nrf2 pathway,where UVB-induced TRPV4 activation suppressed Nrf2 activity and promoted AP-1-mediated inflammation.By reducing TRPV4 overexpression and restoring Nrf2 function,LJGp alleviated barrier dysfunction and improved epidermal repair.These results provide supporting data and a scientific basis for the development and utilization of LJGp as an epidermal photoinflammatory relief agent. 展开更多
关键词 Laminaria japonica Fermented polysaccharides Epidermal photoinflammation Skin barrier TRPV4-Keap-1/Nrf2 signaling pathway Ganoderma lucidum
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Fermentation Production of Ganoderma lucidum by Bacillus subtilis Ameliorated Ceftriaxone-induced Intestinal Dysbiosis and Improved Intestinal Mucosal Barrier Function in Mice 认领 引用 被引量：5
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作者 LIU He FAN Qing-Jie +2 位作者 LIU Yin-Hui LI Ming YUAN Jie-Li 《Digital Chinese Medicine》 CAS 2020年第3期199-212,共14页
Objective This study aimed to develop a type of Ganoderma lucidum(G.lucidum)-probiotic fermentation broth that can effectively improve the intestinal mucosal barrier function of mice with ceftriaxone-induced intestina... Objective This study aimed to develop a type of Ganoderma lucidum(G.lucidum)-probiotic fermentation broth that can effectively improve the intestinal mucosal barrier function of mice with ceftriaxone-induced intestinal dysbiosis.Methods By means of absorbance of optical density(OD)value and phenol-concentrated sulfuric acid measurement of polysaccharide content,the probiotic species can grow on the medium of G.lucidum were screened out,and the concentration of the medium of G.lucidum was determined,and the fermentation broth was prepared for subsequent experiments.Thirty-two SPF grade male BALB/c mice were randomly divided into four groups(eight mice in each group),namely control group(CON),intestinal mucosal barrier damage model group(CS),fermentation broth intervention group(FT)and G.lucidum medium intervention group(GL),respectively.The intestinal dysregulation model was induced by intra-gastric administration of 0.2 mL ceftriaxone sodium(twice a day for seven consecutive days).From day 8,the FT group and GL group were gavage with 0.2 mL fermentation broth and G.lucidum medium,respectively.On day 15,all mice were sacrificed.To draw the weight curve and measure the cecal index;pathological examination of colon tissues with HE staining;serum levels of LPS,IL-10,TNF and IL-6 were detected by ELISA.Flow cytometry was used to analyze the changes of CD3+T cells,CD4+T cells,CD8+T cells and macrophages in spleen.16S rRNA sequencing was performed to detect the intestinal microbiota structure of mice.Results Bacillus subtilis can decompose and utilize G.lucidum fruiting body medium,and the suitable concentration of G.lucidum fruiting body medium is 33.2 mg/mL.The effect of Bacillus subtilis-G.lucidum fermentation broth on the damage of intestinal mucosal barrier caused by ceftriaxone sodium was reduced,the body weight of mice recovered and colon swelling improved,colon histopathological injury was alleviated,inflammatory cell infiltration was alleviated,serum IL-10 increased significantly,LPS、TNF-αand IL-6 decreased significantly compared with model group,and the proportion of T cells and intestinal dysbiosis was improved.Conclusions The experimental results suggest that Bacillus subtilis-G.lucidum fermentation broth can effectively improve the intestinal barrier function damage,immune dysfunction and intestinal dysbiosis caused by antibiotic overdose,and has a certain regulatory effect on intestinal mucosal barrier function. 展开更多
关键词 Ganoderma lucidum Bacillus subtilis Traditional Chinese medicine fermentation Ganoderma lucidum acid Intestinal mucosal barrier function Intestinal dysbiosis Regulate Tcell responses
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Ganoderma lucidum:a comprehensive review of phytochemistry,efficacy,safety and clinical study 认领 引用 被引量：17
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作者 Sijia Wu Siyuan Zhang +5 位作者 Bo Peng Dechao Tan Mingyue Wu Jinchao Wei Yitao Wang Hua Luo 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第2期568-596,共29页
Ganoderma lucidum,one of the most well-known edible fungi,is believed to be very beneficial for longevity and vitality.A long usage history suggests that G.lucidum has various clinical therapeutic effects.And experime... Ganoderma lucidum,one of the most well-known edible fungi,is believed to be very beneficial for longevity and vitality.A long usage history suggests that G.lucidum has various clinical therapeutic effects.And experimental studies have confirmed that G.lucidum has multiple pharmacological effects,including antitumor,anti-microbial,anti-HIV protease,and antidiabetic activity and so on.With the deepening of research,more than 300 compounds have been isolated from G.lucidum.There is an increasing population of G.lucidum-based products,and its international development is expanding.Currently,G.lucidum has drawn much attention to its chemical composition,therapeutic effect,clinical value,and safety.This paper provides a comprehensive review of these aspects to enhance the global promotion of G.lucidum. 展开更多
关键词 Ganoderma lucidum Phytochemistry Efficacy Safety Clinical study
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Spectrum-effect relationship of immunologic activity of Ganoderma lucidum by UPLC-MS/MS and component knock-out method 认领 引用 被引量：9
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作者 Changqin Li Yiping Cui +5 位作者 Jie Lu Lijun Meng Changyang Ma Zhenhua Liu Yan Zhang Wenyi Kang 《Food Science and Human Wellness》 SCIE CAS CSCD 2021年第3期278-288,共11页
This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network mode... This study was designed to elucidate the immunoregulation of Ganoderma lucidum.HPLC fingerprint and spectrum-effect relationship of G.lucidum were established to predict the active compounds and BP neural network model was established to predict the efficacy.Then the target compounds were identified by high resolution mass spectrometry.The results indicated that there are both enhanced immunity and immunosuppressive components in G.lucidum.BP neural network was trained with the common peak area and immune effi cacy index of G.lucidum fi ngerprint as samples,and a combined evaluation system of G.lucidum fi ngerprint effi cacy was established.The correlation coeffi cient R of BP network model was 0.98643,and the error of pharmacodynamic prediction results was in the ideal range.Eight compounds were identifi ed by high resolution mass spectrometry.The compounds related to immune activity in G.lucidum were determined in this study. 展开更多
关键词 Ganoderma lucidum RAW 264.7 cell Spectrum-effect relationships Component knock-out BP neural network
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In vitro and in vivo protective effects of proteoglycan isolated from mycelia of Ganoderma lucidum on carbon tetrachlorideinduced liver injury 认领 引用 被引量：13
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作者 Xiao-Jun Yang Jing Liu +4 位作者 Lin-Bai Ye Fan Yang Li Ye Jin-Rong Gao Zheng-Hui Wu 《World Journal of Gastroenterology》 SCIE CAS 2006年第9期1379-1385,共7页
AIM： To investigate the possible mechanism of the protective effects of a bioactive fraction, Ganoderma lucidum proteoglycan （GLPG）isolated from Ganoderma luddum mycelia, against carbon tetrachloride-induced liver ... AIM： To investigate the possible mechanism of the protective effects of a bioactive fraction, Ganoderma lucidum proteoglycan （GLPG）isolated from Ganoderma luddum mycelia, against carbon tetrachloride-induced liver injury. METHODS： A liver injury model was induced by carbon tetrachloride. Cytotoxicity was measured by MTY assay. The activities of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were determined with an automatic multifunction-biochemical analyzer and the levels of superoxide dismutase （SOD） and TNF-α were determined following the instructions of SOD kit and TNF radioimmunoassay kit. Uver sections were stained with hematoxylin and eosin （H＆E） for histological evaluation and examined under light microscope. RESULTS： We found that GLPG can alleviate the L-02 liver cells injury induced by carbon tetrachloride （CCh） through the measurements of ALT and AST activities and the administration of GLPG to L-02 cells did not display any toxicity. Furthermore, histological analysis of mice liver injury induced by CCh with or without GLPG pretreatment indicated that GLPG can significantly suppress the toxicity induced by CCh in mice liver. We also found that GLPG reduced TNF-α level induced by CCh in the plasma of mice, whereas increased SOD activity in the rat serum. CONCLUSION： GLPG has hepatic protective activity against CCl4 induced injury both in vitro and in vivo. The possible antihepatotoxic mechanisms may be related to the suppression of TNF-α level and the free radical scavenging activity. 展开更多
关键词 Ganoderma lucidum proteoglycan （GLPG） Carbon tetrachloride （CCl4） Uver injury Hepatic protective activity
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Submerged fermentation production and characterization of intracellular triterpenoids from Ganoderma lucidum using HPLC-ESI-MS 认领 引用 被引量：20
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作者 Mei-lin CUI Huan-yi YANG Guo-qing HE 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2015年第12期998-1010,共13页
As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were ... As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% （0.041 g/ml） and yeast extract 1.89% （0.0189 g/ml）, pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry （HPLC-ESI-MS）, 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H20 and CO2, as well as cleavage of the D-ring. 展开更多
关键词 Ganoderma lucidum Wort Response surface methodology Ganoderic acids HPLC-ESI-MS
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Ganoderma lucidum spore oil(GLSO),a novel antioxidant,extends the average life span in Drosophila melanogaster 认领 引用 被引量：8
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作者 Yi Zhang Hongfei Cai +5 位作者 Zhu Tao Cheng Yuan Zhaojian Jiang Juyan Liu Hiroshi Kurihara Wendong Xu 《Food Science and Human Wellness》 SCIE CAS CSCD 2021年第1期38-44,共7页
In ancient China,Ganoderma lucidum was believed to be a medical fungus that could increase lifespan.Recently,pharmacologic studies have found that polysaccharide peptides and triterpenoids extracted from Ganoderma luc... In ancient China,Ganoderma lucidum was believed to be a medical fungus that could increase lifespan.Recently,pharmacologic studies have found that polysaccharide peptides and triterpenoids extracted from Ganoderma lucidum have various physiological effects as active compounds.However,the effects of spore oil isolated from Ganoderma lucidum remains unknown.In this study,the biological effects of Ganoderma lucidum spore oil(GLSO)were evaluated using a Drosophila melanogaster model.Compared with untreated groups,groups treated with GLSO had significantly longer average and maximum lifespan in both normal conditions and under oxidative stress.The activities of various antioxidant enzymes were measured to determine the antioxidant effect of GLSO.GLSO treatment markedly enhanced total superoxide dismutase(SOD)and catalase(CAT)activity and decreased levels of malondialdehyde(MDA).Further,we found dose-dependent increases in the mRNA expression of Cu,Zn-SOD,Mn-SOD,and CAT in GLSO-treated groups.These results suggest that GLSO may effectively eliminate free radicals and extend lifespan in Drosophila.Future work should investigate the value of GLSO as a functional food for the prevention of aging in larger animal models. 展开更多
关键词 Ganoderma lucidum spore oil Antioxidant activity Drosophila melanogaster Anti-aging
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Apoptosis induction of colorectal cancer cells HTL-9 in vitro by the transformed products of soybean isoflavones by Ganoderma lucidum 认领 引用 被引量：10
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作者 Mei-lin CUI Huan-yi YANG Guo-qing HE 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2017年第12期1101-1112,共12页
Soybean isoflavones have been one of the potential preventive candidates for antitumor research in recent years. In this paper, we first studied the transformation of soybean isoflavones with the homogenized slurry of... Soybean isoflavones have been one of the potential preventive candidates for antitumor research in recent years. In this paper, we first studied the transformation of soybean isoflavones with the homogenized slurry of Ganoderma lucidum. The resultant transformed products（TSI） contained（703.21±4.35） mg/g of genistein, with transformed rates of 96.63% and 87.82% of daidzein and genistein, respectively, and TSI also could enrich the bioactive metabolites of G. lucidum. The antitumor effects of TSI on human colorectal cancer cell line HTL-9, human breast cancer cell line MCF-7, and human immortalized gastric epithelial cell line GES-1 were also studied. The 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide（MTT） assay showed that TSI could dramatically reduce the viability rates of HTL-9 cells and MCF-7 cells without detectable cytotoxicity on GES-1 normal cells when the TSI concentration was lower than 100 μg/ml. With 100 μg/ml of TSI, HTL-9 cells were arrested in the G1 phase, and late-apoptosis was primarily induced, accompanied with partial early-apoptosis. TSI could induce primarily earlyapoptosis by arresting cells in the G1 phase of MCF-7 cells. For HTL-9 cells, Western-blot and reverse-transcriptase polymerase chain reaction（RT-PCR） analysis showed that TSI（100 μg/ml） can up-regulate the expression of Bax, Caspase-3, Caspase-8, and cytochrome c（Cyto-c）, indicating that TSI could induce cell apoptosis mainly through the mitochondrial pathway. In addition, the expression of p53 was up-regulated, while the expression of Survivin and nuclear factor κB（NF-κB） was down-regulated. All these results showed that TSI could induce apoptosis of HTL-9 cells by the regulation of multiple apoptosis-related genes. 展开更多
关键词 Soybean isoflavones Ganoderma lucidum Transformation Antiproliferative activity Apoptosis
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GPP(composition of Ganoderma lucidum polysaccharides and Polyporus umbellatus polysaccharides) protects against DSS-induced murine colitis by enhancing immune function and regulating intestinal flora 认领 引用 被引量：6
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作者 Liyuan Li Yannan Guo +6 位作者 Qing Huang Xiaojin Shi Qingqing Liu Fang Wang Qingfei Liu Kang Yu Zhao Wang 《Food Science and Human Wellness》 SCIE CAS CSCD 2022年第4期795-805,共11页
Previous study have demonstrated that a compound composed of water-soluable Ganoderma lucidum polysaccharides(GLP)and Polyporus umbellatus polysaccharides(PUP)in a ratio of 3:1 named GPP enhances innate immune functio... Previous study have demonstrated that a compound composed of water-soluable Ganoderma lucidum polysaccharides(GLP)and Polyporus umbellatus polysaccharides(PUP)in a ratio of 3:1 named GPP enhances innate immune function in mice through enhancing the function of macrophage cells and activity of natural killer(NK)cells.Here in our research,we further investigated the effect of GPP on the diversity and composition of intestinal flora,and explored its effect on colitis model mice.The immunoregulatory verification experiments of GPP were conducted in both normal and DSS-induced mice model.Our research showed that GPP increased the diversity of intestinal microorganisms in mice with the extension of administration time.Daily GPP intake attenuated DSS-induced colon injury,protected the splenic lymphocyte proliferation ability,enhanced the serum hemolysin synthesis,and increased peripheral phagocytes and NK cell activity in model mice.Comparisons of the predominant gene pathways of the bacterial microbiota showed that DNA repair and recombination,base mismatch repair pathways was stronger in GPP-treatment group than in control group,indicating the possible molecular mechanisms of immune function regulation.Our study showed that GPP regulated immune function in both health and colitis model,and had a positive effect on maintaining intestinal flora homeostasis. 展开更多
关键词 Ganoderma lucidum polysaccharides Polyporus umbellatus polysaccharides Immune function Intestinal flora Colitis
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SOLUTION PROPERTIES OF ANTITUMOR CARBOXYMETHYLATED DERIVATIVES OFα-(1→3)-D-GLUCAN FROM GANODERMA LUCIDUM 认领 引用 被引量：6
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作者 Li-na Zhang Mei Zhang +1 位作者 Jing-hua Chen Fan-bo Zeng 《Chinese Journal of Polymer Science》 SCIE EI CAS 2001年第3期283-289,共7页
Fractions of a water insolubleα-(1→3)-D-glucan(GL)extracted from Ganoderma lucidum were carboxy-methylated(CM)to obtain water-soluble carboxymethylated derivatives(CM-GL)having a degree of substitution(DS)of0.38~0.5... Fractions of a water insolubleα-(1→3)-D-glucan(GL)extracted from Ganoderma lucidum were carboxy-methylated(CM)to obtain water-soluble carboxymethylated derivatives(CM-GL)having a degree of substitution(DS)of0.38~0.51.Weight-average molecular weight M_w and intrinsic viscosity[η]of the samples CM-GL were measured by gelpermeation chromatography combined with laser light scattering(GPC-LLS)and viscometry.The CM-GL exhibits a stifferchain in aqueous solution at 25℃than the original glucan,The antitumor activities against Ehrlich ascites carcinoma(EAC,5×106)of the carboxymethylated derivatives from theα-glucan and curdlan,aβ-glucan,are significantly higher than thoseof the original glucans.The effects of the relatively low molecular weight,expanded chains and better water-solubility of theCM-GL on the enhancement of antitumor activity could not be neglected.The chain stiffness decreased speedily withincrease of temperature from 40 to 60℃or NaOH concentration from 0.1 to 0.4 in the solution,respectively,and the changeof the chain stiffness is reversible. 展开更多
关键词 Solution properties Antitumor activity Carboxymethylated derivative α-D-glucan Ganoderma lucidum
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Randomized clinical trial of an ethanol extract of Ganoderma lucidum in men with lower urinary tract symptoms 认领 引用 被引量：2
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作者 Masanori Noguchi Tatsuyuki Kakuma +7 位作者 Katsuro Tomiyasu Akira Yamada Kyogo Itoh Fumiko Konishi Shoichiro Kumamoto Kuniyoshi Shimizu Ryuichiro Kondo Kei Matsuoka 《Asian Journal of Andrology》 SCIE CAS 2008年第5期777-785,共9页
Aim： To evaluate the safety and efficacy of an extract of Ganoderma lucidum that shows the strongest 5α-reductase inhibitory activity among the extracts of 19 edible and medicinal mushrooms by a double-blind, placeb... Aim： To evaluate the safety and efficacy of an extract of Ganoderma lucidum that shows the strongest 5α-reductase inhibitory activity among the extracts of 19 edible and medicinal mushrooms by a double-blind, placebo-controlled, randomized and dose-ranging study in men with lower urinary tract symptoms （LUTS）. Methods： In this trial, we randomly assigned 88 men over the age of 49 years who had slight-to-moderate LUTS to 12 weeks of treatment with G. lucidum extract （6 mg once a day） or placebo. The primary outcome measures were changes in the International Prostate Symptom Score （IPSS） and variables of uroflowmetry. Secondary outcome measures included changes in prostate size, residual urinary volume after voiding, laboratory values and the reported adverse effects. Results： G. lucidum was effective and significantly superior to placebo for improving total IPSS with 2.1 points decreasing at the end of treatment （mean difference, -1.18 points; 95% confidence interval, -1.74 to -0.62; P 〈 0.0001）. No changes were observed with respect to quality of life scores, peak urinary flow, mean urinary flow, residual urine, prostate volume, serum prostate-specific antigen or testosterone levels. Overall treatment was well tolerated with no severe adverse effects. Conclusion： The extract of G. lucidum was well tolerated and improved IPSS scores. These results encouraged a further, large-scale evaluation of phytotherapy for a long duration using the extract of G. lucidum on men with LUTS. 展开更多
关键词 lower urinary tract symptoms phytotherapy outcome randomized trial Ganoderma lucidum
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Ganoderma Lucidum Spore Oil enhances the effect of cyclophosphamide via inhibiting programmed death-1 and prolongs the survival of H22 tumor-bearing mice 认领 引用 被引量：2
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作者 JIANG Zhaojian CAI Hongfei +7 位作者 YUAN Cheng CAO Lin XU Wendong HAN Yaming ZHANG Qin LI Jing WANG Qin LIU Juyan 《Journal of Traditional Chinese Medicine》 SCIE CSCD 2024年第4期652-659,共8页
OBJECTIVE:To investigate the effect of Ganoderma Lucidum Spore Oil(GLSO)on the tumor growth and survival of H22 tumor-bearing mice treated with cyclophosphamide(CTX),and explore the underlying mechanism.METHODS:Allogr... OBJECTIVE:To investigate the effect of Ganoderma Lucidum Spore Oil(GLSO)on the tumor growth and survival of H22 tumor-bearing mice treated with cyclophosphamide(CTX),and explore the underlying mechanism.METHODS:Allograft H22 hepatocellular carcinoma mouse model was applied to investigate the effect of GLSO on the tumor growth and survival of animals,and Kaplan-Meier survival analysis was used to analyze the life span.Plasma biochemical examination was used to determine the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),urea(UREA)and creatinine(CRE).Western blot analysis was performed to detect Programmed Death-1(PD-1),Programmed Death Ligand 1(PD-L1),Janus Kinase 2(JAK2),phosphorylated Signal Transducer and Activator of Transcription 3(p-STAT3),and Signal Transducer and Activator of Transcription 3(STAT3)expression.RESULTS:GLSO increased the anti-tumor effect of CTX and prolonged the survival of H22 tumor-bearing mice treated with CTX.Meanwhile,GLSO increased the thymus index and showed no obvious toxicity to liver functions of animals.GLSO also decreased the level of UREA in H22 tumor-bearing mice treated with CTX.Furthermore,GLSO could inhibit the expression of PD-1 in spleen,which was independent of JAK2 expression and STAT3 phosphorylation.However,GLSO did not affect the expression of PD-L1,JAK2,and p-STAT3 in tumor tissue.CONCLUSION:GLSO could strengthen the anti-tumor effect of CTX and prolong the life span of H22 tumorbearing mice,while the underlying mechanism might be relevant to the amelioration effect of thymus function and inhibition of PD-1 expression in spleen.Furthermore,these findings implied the promising role of GLSO in combination with CTX to extend the survival of patients in clinical chemotherapy of hepatocellular carcinoma. 展开更多
关键词 carcinoma,hepatocellular cyclophosphamide survival programmed cell death 1 receptor Ganoderma Lucidum SporeOil
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Antitumor Activity of the Ganoderma Lucidum Spore Alcohol Extract in Vitro 认领 引用 被引量：4
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作者 杨新林 朱鹤孙 +1 位作者 徐建兰 匡群 《Journal of Beijing Institute of Technology》 EI CAS 1997年第4期40-44,共5页
Several cancer cell lines(epithelioma cells or leukemia cells)from human being or mouse were first used to study the antitumor activity of the Ganoderma lucidum spore alcohol extract(GLSAE)in vitro by the MTT test A c... Several cancer cell lines(epithelioma cells or leukemia cells)from human being or mouse were first used to study the antitumor activity of the Ganoderma lucidum spore alcohol extract(GLSAE)in vitro by the MTT test A comparision was made between the sporodermbroken(SB)and sporoderm nonbroken(SN)GLSAE It was showed that both GLSAE SB and GLSAE SN could inhibit the proliferation of these cancer cells,but the activity of GLSAE SB was much higher than that of GLSAE SN These results suggested that Ganoderma lucidum spore could probably be used for tumor treatment 展开更多
关键词 Ganoderma lucidum spore alcohol extract antitumor activity,cancer cell line
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Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers 认领 引用 被引量：1
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作者 CHAO Wen-zheng TANG Chuan-hong +2 位作者 ZHANG Jing-song YU Ling Honda Yoichi 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第1期130-138,共9页
The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding. Hunong 5 has high commercial value due to its high polysaccharide and triterpene content, This is the first report of using a DNA pooling meth... The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding. Hunong 5 has high commercial value due to its high polysaccharide and triterpene content, This is the first report of using a DNA pooling method to develop a stable sequence characterized amplified region （SCAR） marker for rapid identification of the G. lucidum Hunong 5 cultivar. The SCAR marker was developed by first generating and sequencing a distinctive inter simple sequence repeat （ISSR） fragment （882 bp） from G. lucidum Hunong 5 cultivar. A stable SCAR primer pair GLH5F/GLH5R were obtained to identify the cultivar and the SCAR marker is a DNA fragment of 773 bp. 展开更多
关键词 DNA pooling Ganoderma lucidum Hunong 5 cultivar ISSR marker SCAR marker
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Studies on Active Polysaccharides from Ganoderma lucidum vith Immune Activity 认领 引用 被引量：5
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作者 何云庆 李荣芷 +3 位作者 陈琪 林志彬 夏冬 马莉 《Journal of Chinese Pharmaceutical Sciences》 CAS 1992年第1期79-81,共3页
从云头状灵芝[Ganoderma Lucidum(Leyss.ex Fr.)Karst.]中分离得到三种灵芝多糖BN3A，BN3B及BN3C，它们均表现明显免疫调节作用。从灵芝多糖BN3B及BN3C中各分离得到四个多糖均一体，对其中主要成分BN3C1，BN3C3，BN3B1及BN3B3进行了物... 从云头状灵芝[Ganoderma Lucidum(Leyss.ex Fr.)Karst.]中分离得到三种灵芝多糖BN3A，BN3B及BN3C，它们均表现明显免疫调节作用。从灵芝多糖BN3B及BN3C中各分离得到四个多糖均一体，对其中主要成分BN3C1，BN3C3，BN3B1及BN3B3进行了物理及化学研究，它们的平均分子量依次为1.6×10^4，2.5×10^4，3.5×10^4及4.0×10^4。经完全酸水解、红外光谱测定、过碘酸氧化、甲酸生成、Smith降解等证明，BN3B1及BN3C1均为β－（1→6)(1→3）甙键相连的葡聚糖。BN3B3为阿拉伯半乳聚糖。BN3C3为由葡萄糖和阿拉伯糖组成的肽多糖。它们均为β－（1→6）（1→3）甙键相连。 展开更多
关键词 Ganoderma lucidum Ganoderma polysaccharides Immune activity
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Effects of Ganoderma lucidum spore powder on astrocyte expression and glutamine synthetase activity in the hippocampal region of epileptic rats 认领 引用 被引量：2
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作者 Shiling Zhang Shuqiu Wang 《Neural Regeneration Research》 SCIE CAS 2008年第12期1304-1307,共4页
BACKGROUND： Recent studies have demonstrated that astrocyte dysfunction plays a central role in inhibiting epileptic seizures and that regulation of astrocyte function may be a new target for treatment of epilepsy. O... BACKGROUND： Recent studies have demonstrated that astrocyte dysfunction plays a central role in inhibiting epileptic seizures and that regulation of astrocyte function may be a new target for treatment of epilepsy. OBJECTIVE： To observe the effects of Ganoderma lucidum spore powder （GLSP） on astrocyte morphology and glutamine synthetase （COS） activity in the hippocampal region of epileptic rats. DESIGN, TIME AND SETTING： A randomized, controlled animal experiment was performed at the Function Laboratory, College of Basic Medicine, Jiamusi University between October and December 2006. MATERIALS： A total of 30 Sprague Dawley （SD） rats were randomized to three groups （n = 10）： control, model, and GLSP. GLSP was sourced from Jiamusi Wild Ganoderma Lucidum Planting Base and prepared to 30 g/L with physiological saline before use. Pentylenetetrazol （PTZ） （10 g/L） was provided by Sigma Company, USA. METHODS： The control group received intraperitoneal （i.p.） and intragastric （i.g.） physiological saline. Following epilepsy induction by i.p. administration of PTZ （35 mg/kg）, rats from the mode/and GLSP groups were ig injected with physiological saline and GLSP （300 mg/kg）, respectively. Each compound was administered once per day, for a total of 28 successive days. Epileptic seizure convulsions were graded 0-5. A higher grade indicated more severe epilepsy. Only those rats showing stage 2 or higher convulsions at least 5 times successively were included in further experiments. MAIN OUTCOME MEASURES： Immediately alter injection, seizure activity was monitored for 30 minutes to determine the latent period and seizure duration; simultaneously, astrocyte numbers and GS activity in the hippocampal region of rats with epilepsy were detected by immunohistochemistry. RESULTS： All 30 rats were included in the final analysis. On day 28, following PTZ administration epileptic seizures were not found in the control group. In the GLSP group, rats exhibited rhythmic head nodding or facial spasms, and the latent period was significantly longer than that of the model group （P 〈 0.05）. In the model group, the majority of rats exhibited myoclonus or generalized convulsions, and epileptic seizure duration was slightly （but not significantly） longer than that in the GLSP group （P 〉 0.05）. Within 2-3 minutes of PTZ injection, the model group exhibited grade 4 or 5 epileptic seizures, and the GLSP group mostly showed grade 2 or 3, and occasionally grade 4 or 5, epileptic seizures. All rats recovered within 30 minutes. The model group exhibited significantly increased astrocytes （P 〈 0.05）, with thicker cellular processes and a higher number of cellular processes, and significantly decreased GS activity （P 〈 0.05） tban the control group. The astrocyte count was significantly decreased but GS activity was significantly increased in the GLSP group when compared with the model group （P 〈 0.05）; however, astrocyte appearance was similar in both groups （P 〈 0.05）. CONCLUSION： GLSP can effectively inhibit astrocyte numbers and elevate GS activity in the hippocampal region of rats with epilepsy, thereby reducing epileptic seizures. 展开更多
关键词 Ganoderma lucidum spore powder astrocyte glutamine synthetase epilepsy
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Renal protective effect of Ganoderma lucidum 认领 引用 被引量：1
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作者 Dan-dan ZHONG Zheng-wei XIE +6 位作者 Bo-yue HUANG Shuai ZHU Guo-qian WANG Hong ZHOU Shu-qian LIN Zhi-bin LIN Bao-xue YANG 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2018年第4期254-255,共2页
OBJECTIVE Non-alcoholic fatty liver disease(NAFLD) encompasses a series of patho.logic changes ranging from steatosis to steatohepatitis,which may progress to cirrhosis and hepatocel.lular carcinoma.The purpose of thi... OBJECTIVE Non-alcoholic fatty liver disease(NAFLD) encompasses a series of patho.logic changes ranging from steatosis to steatohepatitis,which may progress to cirrhosis and hepatocel.lular carcinoma.The purpose of this study was to determine whether Ganoderma lucidum polysaccha.ride peptide(GLPP) has therapeutic effect on NAFLD.METHODS ob/ob mouse model and ApoC3 transgenic mouse model were used for exploring the effect of GLPP on NAFLD.Key metabolic path.ways and enzymes were identified by metabolomics combining with KEGG and PIUmet analyses and key enzymes were detected by Western blotting.Hepatosteatosis models of HepG2 cells and primary hepatocytes were used to further confirm the therapeutic effect of GLPP on NAFLD.RESULTS GLPP administrated for a month alleviated hepatosteatosis,dyslipidemia,liver dysfunction and liver insulin resistance.Pathways of glycerophospholipid metabolism,fatty acid metabolism and primary bile acid biosynthesis were involved in the therapeutic effect of GLPP on NAFLD.Detection of key enzymes revealed that GLPP reversed low expression of CYP7 A1,CYP8 B1,FXR,SHP and high expression of FGFR4 in ob/ob mice and ApoC3 mice.Besides,GLPP inhibited fatty acid synthesis by reducing the expression of SREBP1 c,FAS and ACC via a FXR-SHP dependent mechanism.Additionally,GLPP reduced the accumulation of lipid droplets and the content of TG in HepG2 cells and primary hepato.cytes induced by oleic acid and palmitic acid.CONCLUSION GLPP significantly improves NAFLD via regulating bile acid synthesis dependent on FXR-SHP/FGF pathway,which finally inhibits fatty acid synthesis,indicating that GLPP might be developed as a therapeutic drug for NAFLD. 展开更多
关键词 Ganoderma lucidum polysaccharide peptide NAFLD insulin resistance hepatosteatosis nuclear receptors
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Determination of ganoderic acids in rat plasma after oral administration of total triterpenoids from Ganoderma lucidum 认领 引用 被引量：2
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作者 薛彬彬 郭晓宇 车庆明 《Journal of Chinese Pharmaceutical Sciences》 CAS 2008年第3期236-240,共5页
To investigate the absorption of total triterpenoids from Ganoderma lucidum in rats. HPLC-DAD and LC-MS methods were used to identify ganoderic acids in rat plasma after oral administration of total triterpenoids from... To investigate the absorption of total triterpenoids from Ganoderma lucidum in rats. HPLC-DAD and LC-MS methods were used to identify ganoderic acids in rat plasma after oral administration of total triterpenoids from G. lucidum by comparing their HPLC retention behaviors, UV absorption spectra, and mass spectra with authentic samples. Five ganoderic acids, ganoderic acid C2, C6, G, B and A were simultaneously detected in rat plasma. Ganoderic acids can be directly absorbed into circulation after oral administration of total triterpenoids from G. lucidum in rats. 展开更多
关键词 Ganoderma lucidum Triterpenoids Ganoderic acids LC-MS HPLC-DAD
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Heme oxygenase-1/carbon monoxide signaling participates in the accumulation of triterpenoids of Ganoderma lucidum 认领 引用 被引量：1
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作者 Meilin CUI Yuchang MA Youwei YU 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2021年第11期941-953,共13页
Ganoderic triterpenoids(GTs)are the primary bioactive constituents of the Basidiomycotina fungus,Ganoderma lucidum.These compounds exhibit antitumor,anti-hyperlipidemic,and immune-modulatory pharmacological activities... Ganoderic triterpenoids(GTs)are the primary bioactive constituents of the Basidiomycotina fungus,Ganoderma lucidum.These compounds exhibit antitumor,anti-hyperlipidemic,and immune-modulatory pharmacological activities.This study focused on GT accumulation in mycelia of G.lucidum mediated by the heme oxygenase-1(HO-1)/carbon monoxide(CO)signaling.Compared with the control,hemin(10μmol/L)induced an increase of 60.1%in GT content and 57.1%in HO-1 activity.Moreover,carbon monoxide-releasing molecule-2(CORM-2),CO donor,increased GT content by 56.0%and HO-1 activity by 18.1%.Zn protoporphyrin IX(ZnPPIX),a specific HO-1 inhibitor,significantly reduced GT content by 26.0%and HO-1 activity by 15.8%,while hemin supplementation reversed these effects.Transcriptome sequencing showed that HO-1/CO could function directly as a regulator involved in promoting GT accumulation by regulating gene expression in the mevalonate pathway,and modulating the reactive oxygen species(ROS)and Ca2+pathways.The results of this study may help enhance large-scale GT production and support further exploration of GT metabolic networks and relevant signaling cross-talk. 展开更多
关键词 Ganoderma lucidum Triterpenoid Heme oxygenase-1(HO-1)/carbon monoxide(CO)signaling Transcriptome sequencing
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