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Structural evolution, chemical and antioxidant properties of pectic polysaccharides from sesame hull roasted at various temperatures 认领 引用
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作者 Jinyuan Hu Xu Fang +6 位作者 Huixian Guo Rui Wang Wenting Yin Xiaoshuang Cai Huamin Liu Xinliang Zhu Xuede Wang 《Grain & Oil Science and Technology》 EI CAS 2026年第1期21-29,共9页
Roasting constitutes a critical stage in sesame oil production,where the thermal degradation of pectic polysaccharide initiates fundamental chemical transformations including caramelization,Maillard processes,lipid ox... Roasting constitutes a critical stage in sesame oil production,where the thermal degradation of pectic polysaccharide initiates fundamental chemical transformations including caramelization,Maillard processes,lipid oxidation and Strecker degradation.This study systematically examines the structural,chemical and oil antioxidant capacity of sesame hull-derived pectic polysaccharides(URA and URB)under 160–220℃,and their impacts on sesame oil oxidative stability.The results demonstrate temperature-dependent molecular restructuring of URA and URB with molecular weights increasing proportionally and reduced total yields by 42.73%until 220℃.Furfural and organic acids were identified as primary pyrolysis products,accompanied by volatile aromatic compounds including furans,benzenes,and phenols.Sesame oil with the mixture of URA and URB(HSO-URA/B)exhibited optimal thermal antioxidant performance,demonstrating improved sesame oil oxidative stability as evidenced by peroxide value(0.07–0.81 g/100 g)and oxidative stability index(22.6 h).This work provides a scientific foundation for optimizing sesame oil quality while transforming hull byproducts into valueadded food ingredients. 展开更多
关键词 Sesame hull Pectic polysaccharides Antioxidant capacity Roasting Degradation
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Suppressive effect of pectic polysaccharides extracted from Rauwolfia verticillata(Lour.) Baill.var.hainanensis Tsiang on inflammation by regulation of NF-κB pathway and interleukin-17 in mice with dextran sulphatesodium-induced ulcerative colitis 认领 引用 被引量：7
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作者 Xin-Pu Miao Xiao-Ning Sun +4 位作者 Lu-Jia Cui Qin-Fang Cao Gui-Feng Zhuang Tao-Zhi Deng Dong-Yan Zhang 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2015年第2期147-152,共6页
Objective:To investigate the effects of pectic polysaccharides extracted from Rauwolfia verticillata(Lour.) Baill.var.hainanensis Tsiang on an experimental murine colitis model.Methods:Experimental colitis was induced... Objective:To investigate the effects of pectic polysaccharides extracted from Rauwolfia verticillata(Lour.) Baill.var.hainanensis Tsiang on an experimental murine colitis model.Methods:Experimental colitis was induced by dextran sulfate sodium(DSS),and mice were divided into 4 groups:control.DSS alone.DSS plus SASP,DSS plus pectic polysaccharides.The disease activity index(DAI) and histological score were observed.The tumor necrosis factor(TNF)-α and interleukin(IL)-17 levels were measured by enzyme-linked immunosorbent assay.I κ B and NF-κB p65 expression were assessed by western blot analysis.Myeloperoxidase(MPO) activity was determined by using MPO assay kit.Re.sults:Administration of pectic polysaccharides significantly reduced the severity of DSS-induced colitis as assessed by DAT and histological score,and resulted in down regulation of MPO activity and NF-κB p65 expression and subsequent degradation of IκB protein,strikingly reduced the production of TNF-α and IL-17.Conclusions:Pectic polysaccharides extracted from Rauvolfia verticillata(Lour.)Baill.var.hainanensis Tsiang exerts beneficial effects in experimental colitis and may therefore provide a useful therapeutic approach for the treatment of UC. 展开更多
关键词 Pectic polysaccharides Ulcerative colitis Nuclear factor Dextran sulfate sodium-induced colitis Interleukin-17
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A novel phenol-bound pectic polysaccharide from Decalepis hamiltonii with multi-step ulcer preventive activity 认领 引用 被引量：6
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作者 BM Srikanta MN Siddaraju Shylaja M Dharmesh 《World Journal of Gastroenterology》 SCIE CAS 2007年第39期5196-5207,共12页
AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar... AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar composition [rhamnose: arabinose: xylose: galactose in the ratio of 16:50:2:32 (w/w), with 141 mg/g of uronic acid] was examined for anti-ulcer potency in vivo against swim/ ethanol stress-induction in animal models. Ulcer index, antioxidant/antioxidant enzymes, H+, K+-ATPase and gastric mucin levels were determined to assess the anti- ulcer potency. Anti-H pylori activity was also determined by viable colony count and electron microscopic studies. RESULTS: SRPP, containing phenolics at 0.12 g GAE/g, prevented stress-induced gastric ulcers in animal models by 80%-85%. Down regulation of gastric mucin 2-3 fold, antioxidant/antioxidant enzymes and upregulation of 3 fold of H+, K+-ATPase in ulcerous animals were normalized upon treatment with SRPP. Histopathological analysis revealed protection to the disrupted gastric mucosal layer and epithelial glands. SRPP also inhibited H+, K+-ATPase in vitro, at an IC50 of 77 μg/mL as opposed to that of 19.3 μg/mL of Lansoprazole and H pylori growth at Minimum Inhibitory Concentration (MIC) of 150 μg/mL. In addition, free radical scavenging (IC50-40 μg/mL) and reducing power (3200 U/g) activities were also observed. CONCLUSION: SRPP, with defined sugar composition and phenolics, exhibited multi-potent free radical scavenging, antioxidant, anti-H pylori, inhibition of H+, K+-ATPase and gastric mucosal protective activities. In addition, SRPP is non-toxic as opposed to other known anti-ulcer drugs, and therefore may be employed as a potential alternative for ulcer management. 展开更多
关键词 Swallow root pectic polysaccharide Gastriculcer H^＋, K^＋-ATPase Histopathology Antioxidant activity H pylori
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Effect of modification technologies on characteristics and bioactivities of the pectic polysaccharide from cantaloupe peels 认领 引用 被引量：1
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作者 Mingze Gong Ruizhan Chen +5 位作者 Hui Sun Helong Bai Cui Yang Dongxue Li Wenjing Wu Yongtang Wang 《Food Bioscience》 SCIE 2025年第9期1238-1255,共18页
In order to further reveal the correlation between characteristics and activities,the effects of separation and modifications(sulfation(SM),ultrasound(UM),high temperature and high pressure(HM),and enzymatic hy-drolys... In order to further reveal the correlation between characteristics and activities,the effects of separation and modifications(sulfation(SM),ultrasound(UM),high temperature and high pressure(HM),and enzymatic hy-drolysis(EM))on the physicochemical characteristics,functional properties,antioxidant,hypoglycemic and hypolipidemic activities of Cantaloupe peels pectin polysaccharides(CPs)were systematically compared.Results showed that the separation and modification techniques were chief factors affecting the yield,chemical composition,molecular weight(Mw),degree of esterification(DE),water solubility(WS),emulsifying ability(EA),emulsion stability(ES),thermal stability,helical structure,antioxidant capacity,hypoglycemic and hypolipidemic activity.Among the eight products,isolated polysaccharide(MCP-1)and HM polysaccharides(HMCP-4)displayed higher antioxidant and hypoglycemic activities,mainly attributed to lower Mw,DE and higher uronic acid content(UAC),proved that Mw and UAC are the main factors affecting the physicochemical char-acteristics and bioactivity of polysaccharides.The results reveal that HM of polysaccharide can significantly change their physicochemical and functional properties,increase bioactivities and broaden the application in food and pharmaceutical industries. 展开更多
关键词 Pectic polysaccharide Modification technology Characteristics Bioactivity Relationship
Pectic polysaccharides from unripe and mature kiwifruit alleviate ulcerative colitis by modulating gut microbiota and restoring the intestinal barrier 认领 引用 被引量：1
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作者 Kai-Xin Jiang Jin-Lei Geng +7 位作者 Qin Yuan Jie Li Wen Liu Hong-Yan Liu Yi-Chen Hu Liang Zou Shengpeng Wang Ding-Tao Wu 《Food Bioscience》 SCIE 2025年第9期3486-3500,共15页
Thinned unripe kiwifruit,a significant byproduct of commercial kiwifruit production,contains abundant pectic polysaccharides comparable to mature kiwifruit.Although acidic heteropolysaccharides from mature kiwifruit e... Thinned unripe kiwifruit,a significant byproduct of commercial kiwifruit production,contains abundant pectic polysaccharides comparable to mature kiwifruit.Although acidic heteropolysaccharides from mature kiwifruit exhibit potent anti-inflammatory activity,the protective effects of unripe kiwifruit-derived pectic polysaccharides against ulcerative colitis(UC)remain unclear,ultimately limiting their potential utilizations.Therefore,to bridge this knowledge gap and facilitate their utilization,the chemical structures and anti-UC effects of two pectic polysaccharides,TKP(from unripe kiwifruit)and MKP(from mature kiwifruit),were systematically studied and compared.Results demonstrated that TKP and MKP share nearly identical primary chemical structures,predominantly comprising homogalacturonan(HG)and rhamnogalacturonan I(RG-I)domains.Particularly,TKP exhibited a longer side chain length(14.37 vs.8.21)and higher RG-I content(73.62 mol%vs.50.02 mol%)than MKP.Furthermore,both TKP and MKP significantly alleviated dextran sulfate sodium(DSS)-induced UC in mice.Notably,TKP exhibited superior effects to MKP in several aspects,such as enhanced butyrate production,more effective restoration of goblet cell populations,enhanced upregulation of tight junction protein ZO-1,and greater reduction in pro-inflammatory cytokines(IL-1β,IL-6,and TNF-α).Both TKP and MKP effectively suppressed pathogenic bacteria(e.g.,Escherichia-Shigella and Oscillibacter)while promoted beneficial bacteria(e.g.,Akkermansia and Bacteroides).Notably,TKP significantly and selectively enhanced Paraprevotella growth,an effect not observed within MKP.These differential effects between TKP and MKP may be linked to their structural differences.Collectively,these findings provide valuable insights to develop TKP as a promising functional food or ingredient for preventing and ameliorating intestinal inflammatory disorders. 展开更多
关键词 Kiwifruit pectic polysaccharide Rhamnogalacturonan I Intestinal inflammation Intestinal barrier function Gut microbiota
Impact of esterification degree of pectic polysaccharides from quinoa microgreen on protective effects against dextran sulfate sodium-induced colitis in mice 认领 引用
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作者 Wang Guo Bo Li +7 位作者 Mao-Qing Wang Yang Cao Qin Yuan Wen Liu Yi-Chen Hu Liang Zou Shengpeng Wang Ding-Tao Wu 《Food Bioscience》 SCIE 2025年第12期1533-1546,共14页
Quinoa microgreen,as a nutrient-dense and health-promoting vegetable,contains abundant rhamnogalacturonan-I enriched pectic polysaccharides.However,their protective effects against ulcerative co-litis(UC)and the influ... Quinoa microgreen,as a nutrient-dense and health-promoting vegetable,contains abundant rhamnogalacturonan-I enriched pectic polysaccharides.However,their protective effects against ulcerative co-litis(UC)and the influence of structural features like degree of esterification(DE)are poorly understood.Consequently,to overcome this gap in understanding,we systematically assessed the anti-UC activity of quinoa microgreen pectic polysaccharides(QMP)exhibiting distinct DE levels:QMP-HDE(28.49%),QMP-MDE(16.8%),and QMP-LDE(4.4%).Results demonstrated that all these pectic polysaccharides significantly mitigated dextran sulfate sodium-induced UC through upregulating tight junction proteins,suppressing intestinal proinflammatory cytokines,promoting short-chain fatty acid production,and resorting gut microbiota homeosta-sis.Notably,low-DE QMP-LDE demonstrated superior efficacy to high-DE QMP-HDE in restoring intestinal barrier integrity(enhanced upregulation of tight junction proteins and reduced serum lipopolysaccharide)and in elevating anti-inflammatory cytokine interleukin-10.Although all pectic polysaccharides suppressed pathogenic genera(Escherichia-Shigella,Klebsiella,and Helicobacter),they differentially modulated beneficial bacteria(Lactobacillus and Muribaculaceae).QMP-HDE selectively enhanced Lactobacillus abundance,whereas QMP-LDE specifically enriched Muribaculaceae.These differential effects among QMP-HDE and QMP-LDE are closely linked to their DE levels.Collectively,our findings shed light on the structure-anti-UC activity relationship of QMP pectic polysaccharides,supporting their potential application as functional foods to prevent intestinal in-flammatory disorders. 展开更多
关键词 Quinoa microgreen Pectic polysaccharides Chemical structure Esterification degree Gut inflammation Intestinal microbiota
Improvement effects of pectic polysaccharide isolated from Saururus chinensis leaves on dextran sulfate sodium-induced ulcerative colitis in BALB/c mice 认领 引用 被引量：3
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作者 Seung-U Son A.Youn Nam +2 位作者 So Min Kim YangKook Rho Kwang-Soon Shin 《Food Bioscience》 SCIE 2022年第6期415-424,共10页
In this study,the improvement effects of oral administration of polysaccharides isolated from Saururus chinensis on a DSS-induced ulcerative colitis mouse model were evaluated.To achieve this,S.chinensis-derived crude... In this study,the improvement effects of oral administration of polysaccharides isolated from Saururus chinensis on a DSS-induced ulcerative colitis mouse model were evaluated.To achieve this,S.chinensis-derived crude polysaccharides(SCP)were extracted using hot water extraction and ethanol precipitation.SCP is composed primarily of eight monosaccharides,including galacturonic acid(46.6%),galactose(10.4%),arabinose(5.6%),and rhamnose(3.8%),that are typical sugar components of pectic polysaccharides.Oral administration of SCP recovered body weight loss and significantly alleviated various ulcerative colitis symptoms such as stool consistency,blood in stool,and blood in the anus.Additionally,the DSS-treated group that exhibited a decrease in colon length,and SCP treatment significantly reduced colon length shortening in a dose-dependent manner.Levels of inflammatory factors such as interleukin-6,tumor necrosis factor-α,and myeloperoxidase were lower in the SCP-treated groups than they were in the DSS-treated group.In contrast,Alcian blue-periodic acid Schiff staining revealed that SCP treatment effectively maintained the amount of mucus and goblet cells in the intestinal lumen compared to that in the DSS-treated group.The short-chain fatty acid content decreased by DSS administration was recovered through oral administration of SCP,and the amount of butyric acid was significantly improved.Consequently,the aforementioned results suggest that SCP isolated from S.chinensis is a potent candidate for use as a new health functional food and pharmaceutical ingredient for colitis treatment. 展开更多
关键词 Saururus chinensis Pectic polysaccharide DSS-Induced ulcerative colitis Anti-Inflammatory
Dietary pectic oligosaccharide supplementation improves rat reproductive performance via regulating intestinal volatile fatty acids during middle gestation 认领 引用 被引量：2
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作者 Minghui Liu Xiangbing Mao +9 位作者 Daiwen Chen Bing Yu Jun He Ping Zheng Jie Yu Junqiu Luo Yuheng Luo Jianping Wang Quyuan Wang Huifen Wang 《Animal Nutrition》 SCIE CAS 2020年第2期210-216,共7页
As a kind of green additive,pectic oligosaccharide(POS) may regulate some physiological functions of animals,such as gut health,antioxidant capacity,immunity and lipid metabolism.This study aimed to identify whether P... As a kind of green additive,pectic oligosaccharide(POS) may regulate some physiological functions of animals,such as gut health,antioxidant capacity,immunity and lipid metabolism.This study aimed to identify whether POS administration can improve maternal reproduction,and to determine the possible metabolism.A total of 48 pregnant Wistar rats randomly allotted into 2 groups,and each group was fed a diet supplemented with 0 or 800 mg/kg of POS.Pectic oligosaccharide administration increased rat born number(P <0.05),did not affect rat embryo number on d 7 of gestation,but increased rat fetus number on d 14 of gestation(P <0.05).On d 14 of gestation,POS treatment improved Lactobacillus and Bifidobacterium populations and volatile fatty acid concentrations of cecal digesta(P <0.05),hormone(progesterone and nitric oxide) and cytokine(interleukin 2) concentrations of serum(P <0.05),and antioxidant capacity of serum(increased total antioxidant capacity and decreased malondialdehyde) and placenta(increased total superoxide dismutase,decreased malondialdehyde)(P <0.05) in pregnant rats.These results suggest that POS administration improved rat reproduction via decreasing fetus loss in middle gestation.This was due to the increased volatile fatty acid concentrations in rat gut improving hormone and inflammatory-cytokine productions,and antioxidant capacity. 展开更多
关键词 Pectic oligosaccharide Rat reproductive performance Volatile fatty acids Hormone and cytokine generations Antioxidant capacity
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Continuous hydrolysis of mango peel pectin for the production of antibacterial pectic oligosaccharides in packed-bed reactor using immobilized polygalacturonase 认领 引用 被引量：2
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作者 Jie Long Xingfei Li +8 位作者 Lin Xue Zhengjun Xie Aiquan Jiao Yuxiang Bai Xing Zhou Long Chen Chao Qiu Xueming Xu Zhengyu Jin 《Food Bioscience》 SCIE 2022年第6期1519-1530,共12页
The aim of this study was to continuously hydrolyze mango peel pectin,an agricultural waste,into pectic oligosaccharides(POS)with antibacterial activity.A packed-bed reactor(PBR)with immobilized polygalacturonase was ... The aim of this study was to continuously hydrolyze mango peel pectin,an agricultural waste,into pectic oligosaccharides(POS)with antibacterial activity.A packed-bed reactor(PBR)with immobilized polygalacturonase was designed,and the effects of the process conditions(flow rate,substrate concentration,and enzyme amount)were evaluated.The optimal conditions were obtained using response surface analysis,and a stable POS production with a yield of 94.56%was achieved.The PBR retained 82%of its initial activity after continuous operation for 72 h.The POS products exhibited good antibacterial activity against E.coli,S.aureus,B.subtilis,and S.typhimurium.The destructive effects of POS on the membrane system integrity were observed via scanning electron microscopy and the leakage of intracellular nucleic acids.Thus,the PBR is a powerful tool for continuous POS production,and hence,it can be applied as an alternative to chemical preservatives in the food industry. 展开更多
关键词 Pectic oligosaccharides(POS) Mango peel pectin Continuous hydrolysis Immobilized polygalacturonase Antibacterial activity
人参果果胶体外消化和酵解特性研究 认领 引用
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作者 焦丽丽 孙茜文 +2 位作者 任婷 姜子烨 李波 《轻工学报》 CAS 北大核心 2026年第2期21-31,共11页
【目的】探究人参果果胶(GBPA)与大鼠肠道菌群的互作机制。【方法】通过建立体外模拟消化-发酵(无菌大鼠粪便)模型,分析GBPA消化特性及其对肠道菌群结构和短链脂肪酸(SCFAs)水平的影响。【结果】在消化阶段,GBPA还原糖(CR)含量由0.041 m... 【目的】探究人参果果胶(GBPA)与大鼠肠道菌群的互作机制。【方法】通过建立体外模拟消化-发酵(无菌大鼠粪便)模型,分析GBPA消化特性及其对肠道菌群结构和短链脂肪酸(SCFAs)水平的影响。【结果】在消化阶段,GBPA还原糖(CR)含量由0.041 mg/mL显著升高至0.163 mg/mL,相对分子质量降低了11.55%,但单糖组成整体无明显变化;发酵48.00 h后,GBPA相对分子质量进一步下降,降幅为51.97%,单糖比例呈现动态变化。此外,GBPA可显著提高拟杆菌属(Bacteroides)、杜博西氏菌属(Dubosiella)和副拟杆菌属(Parabacteroides)相对丰度,分别为对照组的1.30倍、12.03倍和11.48倍;同时使啮齿杆菌属(Rodentibacter)相对丰度降低了82.44%。GBPA还能促进SCFAs生成,进而改善T2DM酯代谢紊乱。【结论】GBPA的结构在消化系统中较稳定,经肠道菌群发酵降解后,主要通过改善菌群结构及促进SCFAs生成来实现益生元效应。 展开更多
关键词 人参果 果胶 消化特性 酵解特性 肠道菌群 短链脂肪酸
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Effect of pectic oligosaccharide on probiotic survival and physicochemical properties of hydrogel beads for synbiotic encapsulation of Lactobacillus bulgaricus 认领 引用 被引量：2
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作者 Ye-eun Lee Yu-Ra Kang Yoon Hyuk Chang 《Food Bioscience》 SCIE 2023年第1期490-497,共8页
The present study was carried out to evaluate the effects of different concentrations of pectic oligosaccharide(POS)on the physicochemical properties of Lactobacillus bulgaricus(L.bulgaricus)loaded-synbiotic hydrogel ... The present study was carried out to evaluate the effects of different concentrations of pectic oligosaccharide(POS)on the physicochemical properties of Lactobacillus bulgaricus(L.bulgaricus)loaded-synbiotic hydrogel beads and the survival of L.bulgaricus.Low methoxyl(LM)pectin-resistant starch(RS)hydrogel beads,named RS beads,were coated/filled with different concentrations of POS:0.8%(RS-POS0.8 beads),1.0%(RS-POS1.0 beads),and 1.2%(RS-POS1.2 beads).POS treatment resulted in smooth beads with less surface dent by hydrogen bonding between RS beads and POS,which was confirmed by SEM and XRD analyses.The encapsulation efficiency of RS beads and RS-POS beads ranged from 90.40%to 92.03%.In simulated gastric fluid(SGF)and bile salt solution,free L.bulgaricus completely died but L.bulgaricus encapsulated in RS-POS1.2 beads only slightly decreased.This indicates that RS-POS1.2 beads exhibited higher protective effects on L.bulgaricus under SGF condition and bile salts.Furthermore,considering the relatively higher storage stability of L.bulgaricus loaded in RS-POS1.2 beads,the present results suggest that RS beads coated/filled with POS could be used for synbiotic encapsulation,protection,and delivery of probiotics. 展开更多
关键词 Pectic oligosaccharide Synbiotic encapsulation Hydrogel bead Probiotic
The impact of Kakadu Plum(Terminalia ferdinandiana)fruit powder and its pectic oligosaccharides on the growth,survival and antimicrobial activity of probiotic bacteria in milk. 认领 引用 被引量：1
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作者 Batlah Almutairi Mark S.Turner +1 位作者 Mary T.Fletcher Yasmina Sultanbawa 《Food Bioscience》 SCIE 2023年第6期3720-3728,共9页
Prebiotics are essential for improving the growth and survival of probiotics in products,which leads to main-taining the balance of the gut microbiome for overall health.Therefore,the aim of this study was to develop ... Prebiotics are essential for improving the growth and survival of probiotics in products,which leads to main-taining the balance of the gut microbiome for overall health.Therefore,the aim of this study was to develop a novel source of prebiotic from an Australian native fruit Terminalia ferdinandiana(Kakadu plum[KP])and its pectic oligosaccharide(POS)extract by studying their prebiotic effect on growth,survival and milk acidification of six probiotic bacteria.Lactococcus lactis 537,Lactobacillus acidophilus ATCC 4356,Lactobacillus plantarum 299v,Lactobacillus rhamnosus GG,Lactobacillus reuteri ATCC 55730 and Lactobacillus casei ATCC 393 were cultured in skim milk containing 1%and 3%of KP,or 1%of POS derived from KP pectin or apple pectin.Based on the results,the stimulation effect of KP and KP POS on bacteria growth and acidification of milk was found to be species-dependent.A higher prebiotic potential was shown by 1%KP for most strains,while 3%KP significantly enhanced the antimicrobial activity of nisin-producing Lc.lactis 537 supernatant against Staphylococcus aureus.KP POS was more effective than KP in assisting probiotic survival during storage over 14 days at 4◦C and maintaining the pH value of milk of 6 or above during fermentation.Analysis of the monosaccharides and uronic acids of POS from KP revealed mostly glucose,fructose,galactose,arabinose and galacturonic acid.The KP and its POS could be classified as novel prebiotics which support improved growth,survival and antimicrobial ac-tivity of specific probiotic bacteria,and areas for future research are recommended. 展开更多
关键词 Prebiotic Lactococcus lactis 537 Australian native plant Nisin Pectic oligosaccharide
Optimization of high-pressure processing-assisted extraction of pectic polysaccharides from three berries 认领 引用 被引量：1
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作者 Xinxin Hu Chengxiao Yu +4 位作者 Shokouh Ahmadi Yuying Wang Xingqian Ye Zhiqiang Hou Shiguo Chen 《Food Quality and Safety》 SCIE CAS CSCD 2023年第1期1-12,共12页
Background and objectives:High-pressure processing(HPP)is a promising assistive method to extract pectic polysaccharides with high rhamnogalacturonan I(RG-I)domain and berries are sources of such pectic polysaccharide... Background and objectives:High-pressure processing(HPP)is a promising assistive method to extract pectic polysaccharides with high rhamnogalacturonan I(RG-I)domain and berries are sources of such pectic polysaccharides.This study extracts pectic polysaccharides from goji berry,raspberry,and cranberry,examines how HPP influences the pectic polysaccharide structure of three berries,and provides a basis for the extraction and modification of pectic polysaccharides with specific structure and bioactivity.Materials and methods:An orthogonal test was performed to optimize the HPP-assisted alkali method to extract the high yield and high RG-I content pectic polysaccharides from three berries.Structural information of pectic polysaccharides extracted by the HPP method and conventional methods were compared from the perspectives of monosaccharide composition,molecular weight,Fourier transform infrared spectroscopy,andnuclearmagneticresonancespectroscopy.Results:For raspberry,the optimal conditions consisted of a pressure of 50o MPa,a pH of 13,and a pressure-holding time of 12 min,while the optimal conditions for goji berry and cranberry were both 40o MPa,pH 13,and 15 min.Under the optimal conditions,the yields for goji berry,raspberry,and cranberry were 10.49%,16.63%,and 17.52%,respectively,and RG-l contents were 81.85%,83.30%,and 63.22%,respectively.HPP showed an effect to degrade homogalacturonan backbones and side chains and increase the RG-I content to some extent.Conclusion:HPP-assisted alkali method was revealed to be an efficient method to extract high RG-I content pectic polysaccharides,especially for cranberry,and was a potential method to modify pectic polysaccharide structure in a certain way. 展开更多
关键词 Pectic polysaccharide High-pressure processing Rhamnogalacturonan-I Structural characteristics
Production of pectic extracts from sugar beet pulp with antiproliferative activity on a breast cancer cell line 认领 引用
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作者 Jacqueline CONCHA Caroline WEINSTEIN Maria Elvira ZUNIGA 《Frontiers of Chemical Science and Engineering》 SCIE CSCD 2013年第4期482-489,共8页
In the last years, sugar beet pectins have been the subject of several investigations involving extraction methodologies, chemical composition and functional properties. The structure of pectins, which depends on the ... In the last years, sugar beet pectins have been the subject of several investigations involving extraction methodologies, chemical composition and functional properties. The structure of pectins, which depends on the extraction method, is decisive in their capacity to induce apoptosis on several cancer cell lines like colon, prostate and breast. In this work, sugar beet pectin extraction was performed in the following steps： lipid extraction with hexane, removal of soluble complex carbohydrates and proteins, and enzymatic treatment with amyloglucosidase, protease, and pectinase. The enzymatic treatment was carried out with Rohapect DA6L under the following conditions： 50℃, pH 4.0, 2% enzyme/substrate （E/S） ratio, 15 h, and a solid to liquid ratio of 1 ： 10. The pectic extract showed a degree of polymerization （DP） profile of 55.8% with DP≥7; 4.9% with DP6; 5.8% between DP2 and DP6 ; 4.7% with DP2; and 28.8% with DP1. The pectic extract was examined for its antiproliferative activity on the MCF-7 breast cancer cell line. At a concentration range of 12.5-25 mg/mL the pectic extract killed 80.6% of the cells, exhibiting a higher antiproliferative activity than 4-hydroxytamoxifen （4- OHT）, a classical anticancer drug, which killed 56.5% of the cells. 展开更多
关键词 pectic extracts antiproliferative activity,breast cancer enzymatic treatment
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果胶低聚糖制备、生物活性及其在动物生产中的应用 认领 引用
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作者 张磊 龙淼 《饲料工业》 CAS 北大核心 2026年第11期26-33,共8页
果胶低聚糖是果胶降解后产生的一类低分子量聚合物,也是现今一类极具潜力的新型益生元物质。文章综述了目前果胶低聚糖的主要结构、来源与普适化的制备方法,并着重分析了其生物活性及目前在畜禽等动物生产中的应用。旨在进一步总结其在... 果胶低聚糖是果胶降解后产生的一类低分子量聚合物,也是现今一类极具潜力的新型益生元物质。文章综述了目前果胶低聚糖的主要结构、来源与普适化的制备方法,并着重分析了其生物活性及目前在畜禽等动物生产中的应用。旨在进一步总结其在促进动物生长并改善健康状况的优势,为最大限度地发挥果胶低聚糖功能及价值,促进其在饲料添加剂领域的进一步研究和量产应用提供依据。 展开更多
关键词 果胶低聚糖 功能性低聚糖 制备 生物活性 饲料添加剂
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海南萝芙木果胶多糖通过MAPK/NF⁃κB信号通路调控小鼠溃疡性结肠炎的焦亡机制研究 认领 引用 被引量：6
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作者 王柳丹 陈旭永 +1 位作者 吴海东 苗新普 《海南医科大学学报》 CAS 北大核心 2025年第10期721-731,共11页
目的:研究海南萝芙木果胶多糖(pectic polysaccharides,PPs)对葡聚糖硫酸钠(dextran sulfate sodium salt,DSS)诱导的小鼠溃疡性结肠炎(ulcerative colitis,UC)的治疗作用及其通过介导丝裂原活化蛋白激酶(mitogen⁃activated protein kin... 目的:研究海南萝芙木果胶多糖(pectic polysaccharides,PPs)对葡聚糖硫酸钠(dextran sulfate sodium salt,DSS)诱导的小鼠溃疡性结肠炎(ulcerative colitis,UC)的治疗作用及其通过介导丝裂原活化蛋白激酶(mitogen⁃activated protein kinase,MAPK)/核因子κB(nuclear factor kappa⁃B,NF⁃κB)/核苷酸结合寡聚化结构域样受体蛋白3(nucleotide⁃binding oligomerization domain⁃like receptorprotein 3,NLRP3)信号通路对结肠上皮细胞焦亡的影响。方法:将24只6~8周龄的C57雄性小鼠随机分成3组:正常对照组、模型组、和果胶多糖治疗组,每组8只。除正常组外,其余2组分别予以5%葡聚糖硫酸钠盐(DSS)自由饮用7 d制备UC小鼠模型,成功造模后,果胶多糖治疗组小鼠继续灌胃治疗7 d。记录小鼠体重变化、便血情况和疾病活动指数(DAI),分离完整结肠测量长度;苏木素⁃伊红(HE)染色观察结肠组织病理变化;免疫组化法检测检测Caspase⁃1、IL⁃1β与TNF⁃α的表达,Western blotting检测结肠组织细胞焦亡(Caspase⁃1、IL⁃18、IL⁃1β、NLRP3、Cleaved⁃GSDMD)和MAPK/NF⁃κB通路(p38、p⁃p38、p65、p⁃p65、JNK、p⁃JNK、ERK、p⁃ERK)蛋白表达;通过透射电镜观察细胞焦亡形态;双荧光素酶报告基因实验和ChIP实验检测NF⁃κB p65与NLRP3启动子的结合情况。结果:与模型组相比,PPs治疗组能减缓小鼠体重下降,抑制结肠缩短、减轻结肠病理损伤,DAI评分低于DSS模型组(P<0.05),结肠上皮细胞焦亡相关因子NLRP3、Caspase⁃1、IL⁃1β、IL⁃18、TNF⁃α、Cleaved⁃GSDMD的蛋白表达显著降低(P<0.01),结肠组织中p38、p⁃p38、p65、p⁃p65、JNK、p⁃JNK、ERK、p⁃ERK蛋白的表达显著降低(P<0.05)。透射电镜观察到细胞焦亡形态在部分细胞呈现焦亡的改变;双荧光素酶报告基因实验和ChIP实验证实NF⁃κB p65基因靶向作用于NLRP3基因的启动子区域,PPs通过增强促进NLRP3的转录。结论:PPs可通过MAPK/NF⁃κB信号通路抑制细胞焦亡,从而抑制DSS小鼠结肠炎症;NF⁃κB转录结合NLRP3启动子可能是影响结直肠细胞焦亡的关键机制。 展开更多
关键词 溃疡性结肠炎 果胶多糖 海南萝芙木 MAPK信号通路 核因子⁃κB 细胞焦亡
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果胶寡糖抑制结肠癌细胞DLD-1增殖及其机制研究 认领 引用
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作者 许云舒 徐田田 +3 位作者 龙明鑫 李佳琪 杨婷 许青松 《大连民族大学学报》 CAS 2025年第3期193-196,247,共4页
为了探讨果胶寡糖抑制人结肠癌细胞DLD-1的增殖作用及其相关机制,采用CCK-8法检测果胶寡糖对细胞增殖的抑制作用,流式细胞仪检测果胶寡糖诱导结肠癌细胞的凋亡情况,RT-PCR方法检测细胞中抗凋亡基因表达,Western blot方法检测细胞中抗凋... 为了探讨果胶寡糖抑制人结肠癌细胞DLD-1的增殖作用及其相关机制,采用CCK-8法检测果胶寡糖对细胞增殖的抑制作用,流式细胞仪检测果胶寡糖诱导结肠癌细胞的凋亡情况,RT-PCR方法检测细胞中抗凋亡基因表达,Western blot方法检测细胞中抗凋亡蛋白(Bcl-2)和促凋亡蛋白(Bax和Caspase-3)的表达。实验结果标明:浓度为1000μg·mL-1的果胶寡糖处理DLD-1细胞48 h后,细胞活力下降到61%,细胞凋亡率为31.67%;果胶寡糖通过降低Bcl-2蛋白表达水平,升高Bax蛋白的表达水平,提高Bax/Bcl-2比率,激活Caspase-3蛋白诱导DLD-1细胞的凋亡,发挥抑制结肠癌细胞的作用。为开发新型抗结肠癌药物提供一定理论依据。 展开更多
关键词 果胶寡糖 人结肠癌 DLD-1细胞 凋亡 Bax蛋白
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黄秋葵嫩果黄酮与果胶多糖分级制备工艺研究 认领 引用
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作者 单纪苗 董晓弟 +4 位作者 刘丽清 张舜琪 王荣春 鲁斌 孟兆青 《农产品加工》 2025年第14期38-44,共7页
以黄秋葵嫩果鲜品为原料,研究黄酮与果胶多糖的分级制备工艺。通过对不同长度的黄秋葵嫩果中黄酮类功效成分进行检测,筛选原料长度范围。在单因素试验基础上,通过正交试验研究黄酮醇提与果胶多糖水提工艺。结果表明,原料最佳长度为10~13... 以黄秋葵嫩果鲜品为原料,研究黄酮与果胶多糖的分级制备工艺。通过对不同长度的黄秋葵嫩果中黄酮类功效成分进行检测,筛选原料长度范围。在单因素试验基础上,通过正交试验研究黄酮醇提与果胶多糖水提工艺。结果表明,原料最佳长度为10~13 cm,黄酮乙醇提取的最佳工艺为料液比1∶2(g∶mL),乙醇体积分数80%,提取时间1 h,提取次数2次;果胶多糖水提取最佳工艺为料液比1∶4,提取时间1 h,提取次数3次。黄酮及果胶多糖提取物出膏率分别为4.4%和1.4%。此种鲜品分级提取方式能较好地保留功效成分,提高原料利用率,为黄秋葵嫩果鲜品深加工及功效成分开发利用提供参考。 展开更多
关键词 黄秋葵嫩果 鲜品 黄酮 果胶多糖 分级提取
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Pectate lyase is a factor in the adaptability for Heterodera glycines infecting tobacco 认领 引用 被引量：5
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作者 TIAN Zhong-ling SHI Hong-li +1 位作者 Munawar Maria ZHENG Jing-wu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第3期618-626,共9页
The soybean cyst nematode, Heterodeara glycines, is a serious pathogen of soybean, and reported to be the host of a wide range of Fabaceae. In the present study, the host specificity and reproductivity of two populati... The soybean cyst nematode, Heterodeara glycines, is a serious pathogen of soybean, and reported to be the host of a wide range of Fabaceae. In the present study, the host specificity and reproductivity of two populations of H. glycines collected from soybean and tobacco were identified and characterized. The comparative identity between β-1,4-endoglucanase, pectate lyase and chorismate mutase of H. glycines parasitizing on soybean and tobacco were 99, 97 and 98%, respectively. The qR T-PCR analysis indicated that the expression of pectate lyase 2 gene was significantly higher in second-stage juveniles of H. glycines Henan population parasitizing on tobacco than that of H. glycines Shanxi population parasitizing on soybean. In addition, the pectic acid content of cell wall was significantly higher(45%) in the roots of tobacco than the roots of soybean. Our results indicate that the changes in transcript parasitism genes may be a result of long-term evolution illustrating how a plant-parasitic nematode adapts to the host environment for optimal infestation and survival. 展开更多
关键词 s: Heterodera glycines tobacco parasitism genes β-1,4-endoglucanase pectate lyase chorismate mutase pectic acid
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基于遗传算法和间隔偏最小二乘的苹果硬度特征波长分析研究 认领 引用 被引量：27
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作者 屠振华 籍保平 +3 位作者 孟超英 朱大洲 史波林 庆兆珅 《光谱学与光谱分析》 SCIE EI CAS 北大核心 2009年第10期2760-2764,共5页
利用傅里叶近红外光谱(FT-NIRS)测定了苹果的硬度。通过使用几种基于遗传算法和间隔偏最小二乘法的特征波长选取方法,包括动态向后间隔偏最小二乘(dynamic backward version of interval PLS,dynamic biPLS)、动态向后间隔偏最小二乘结... 利用傅里叶近红外光谱(FT-NIRS)测定了苹果的硬度。通过使用几种基于遗传算法和间隔偏最小二乘法的特征波长选取方法,包括动态向后间隔偏最小二乘(dynamic backward version of interval PLS,dynamic biPLS)、动态向后间隔偏最小二乘结合遗传算法(dynamic biPLS & GA-PLS)和反复的遗传算法(iterative GA-PLS),分析了苹果硬度的特征波长。结果表明,运用遗传算法和间隔偏最小二乘选择特征波长后,不但可以降低模型的复杂度,同时能够达到提高模型预测精度的效果。在此基础上,研究分析了苹果硬度特征波长的物理化学意义。由于果胶是在苹果成熟过程中一种和硬度有很大关联的物质,通过比较苹果硬度的特征波长和果胶的特征吸收峰,发现两者具有有很好的一致性。因此,采用遗传算法和间隔偏最小二乘法得到的苹果硬度的特征波长能够反映果胶的吸收信息,从而解释了近红外技术检测苹果硬度的机理。 展开更多
关键词 近红外光谱 硬度 遗传算法 间隔偏最小二乘法 苹果 果胶
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