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GEPS of meat-derived Pediococcus pentosaceus LL-07 displays potential probiotic properties against dextran sulfate-induced colonic damage in mice 认领 引用
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作者 Kuan Lu Xueya Wang +1 位作者 Ying Zhou Qiujin Zhu 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第5期2184-2197,共14页
Exopolysaccharides(EPS)have attracted attention due to their potential role in intestinal repair and stabilization of gut microorganisms.This study aims to investigate the influences of EPS(GEPS)produced by Pediococcu... Exopolysaccharides(EPS)have attracted attention due to their potential role in intestinal repair and stabilization of gut microorganisms.This study aims to investigate the influences of EPS(GEPS)produced by Pediococcus pentosaceus LL-07 on dextran sodium sulfate(DSS)-induced colitis and to reveal the underlying mechanisms.Results showed that GEPS intervention significantly reduced weight loss and colon length shortening in colitis mice,and suppressed the expression of inflammation-related enzymes(myeloperoxidase(MPO),cyclooxygenase-2(COX-2),and inducible nitric oxide synthase(iNOS)),and pro-inflammatory cytokines(interleukin 6(IL-6),tumor necrosis factor-alpha(TNF-α),and IL-1β).The anti-inflammatory factor IL-10,malondialdehyde,and peroxidation stress factors(catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)),were increased.The tight junction proteins(Occludin,Claudin-3,and ZO-1)associated with the intestinal barrier were also increased.The gut microbiota was significantly improved,with an increase in the beneficial bacteria(Ligilactobacillus,Lactobacillus,Alloprevotella,etc.)and a decrease in the harmful bacteria(EscherichiaShigella,etc.).In conclusion,GEPS can improve DSS-induced colitis by inhibiting inflammatory responses,maintaining the intestinal barrier,and regulating the balance of the gut microbiota.These results help accelerate the development of GEPS as a new agent for the treatment of colitis. 展开更多
关键词 Pediococcus pentosaceus Exopolysaccharide Ulcerative colitis Gut microbiota
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SPP1在山羊早期胎盘发育过程中的时序表达及DNA甲基化调控作用 认领 引用
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作者 孙智鹏 程文强 +7 位作者 马骧 罗一鸣 廖宇豪 赵军印 方兴强 杨松建 王明明 赵永聚 《畜牧兽医学报》 CAS CSCD 北大核心 2026年第6期3299-3314,共16页
旨在阐明分泌型磷酸蛋白1(secreted phosphoprotein 1,SPP1)在山羊妊娠早期胎盘组织中的时序表达特征及DNA甲基化调控作用,并探讨DNA甲基化对胎盘滋养层细胞功能的影响。本研究选取健康、体况相近的成年大足黑山羊母羊,按妊娠第20、25... 旨在阐明分泌型磷酸蛋白1(secreted phosphoprotein 1,SPP1)在山羊妊娠早期胎盘组织中的时序表达特征及DNA甲基化调控作用,并探讨DNA甲基化对胎盘滋养层细胞功能的影响。本研究选取健康、体况相近的成年大足黑山羊母羊,按妊娠第20、25、30、45和60天分组,每组3只;采集胎盘组织后,利用苏木精-伊红(H&E)和过碘酸-雪夫(PAS)染色观察胎盘结构发育与滋养层细胞分布,利用免疫组织化学(IHC)、RT-qPCR、Western blot分析SPP1的定位及表达动态,并利用RT-qPCR检测其受体基因的时序表达变化;利用甲基化特异性PCR(MS-PCR)检测不同时期胎盘组织SPP1启动子区甲基化水平;培养并鉴定滋养层细胞,经5-Aza处理后评估DNMT1、DNMT3b、SPP1表达及细胞增殖和侵袭变化。结果显示,随着妊娠进展,胎儿滋养层逐渐由接触转向浅层侵袭,与母体肉阜形成紧密嵌合结构。SPP1在胎儿侧第25天和母体侧第20天胎盘组织中表达最高(P0.05)。细胞试验进一步表明,5-Aza可抑制DNMT1和DNMT3b表达并上调SPP1,同时减弱滋养层细胞增殖能力,促进其侵袭(P<0.05)。综上,SPP1在山羊妊娠早期胎盘中呈现阶段与部位特异性表达模式,与整合素、CD44及Wnt5a等协同参与胚胎附植和胎盘形成,其表达及滋养层细胞功能受DNA甲基化调控。本研究为解析反刍动物胎盘发育机制,以及提升母羊繁殖效率提供了理论依据。 展开更多
关键词 山羊 SPP1 胎盘发育 滋养层细胞 DNA甲基化
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Comparative Analysis of Tumor Microbiota Identifies a Metastatic-Specific Bacterial Signature,Highlighting Streptococcus spp.As the Predominant Hub across Cancers 认领 引用
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作者 Nevena Todorovic Sara Bertorello +6 位作者 Giulia Nannini Serena Pillozzi Simonetta Bianchi Maria Raffaella Ambrosio Elena Niccolai Simone Baldi Amedeo Amedei 《Oncology Research》 SCIE 2026年第5期367-382,共16页
Background:Cancer remains one of the leading global health challenges,with lung cancer(LC),breast cancer(BC),and colorectal cancer(CRC)among the most prevalent and deadly malignancies.The intratumoral microbiota(IM),a... Background:Cancer remains one of the leading global health challenges,with lung cancer(LC),breast cancer(BC),and colorectal cancer(CRC)among the most prevalent and deadly malignancies.The intratumoral microbiota(IM),a distinct microbial ecosystem within tumor tissues,has recently emerged as a potential modulator of carcinogenesis,immune responses,and metastatic progression.However,comparative cross-cancer analyses remain limited.Therefore,this study aimed to compare the IM across these cancer types,with particular emphasis on distinguishing metastatic from non-metastatic malignancies,to identify tumor-specific microbial signatures with potential relevance for biomarker discovery,patient stratification,and microbiota-informed therapeutic strategies.Methods:We performed 16S rRNA gene sequencing to profile the IM in formalin-fixed,paraffin-embedded(FFPE)samples from 20 BC patients,20 CRC patients and 15 non-small cell lung cancer(NSCLC)patients.Results:BC samples exhibited the highest genus-level richness,whereas CRC samples showed significantly greater overall alpha diversity,consistent with the microbial complexity of the gut environment.NSCLC samples displayed the most balanced microbial distribution,as reflected by the highest Shannon index value.Stratification by metastatic status revealed distinct microbial signatures:16 genera were exclusive to metastatic tumors and 49 to non-metastatic ones.In BC specifically,the class Clostridia and the family Burkholderiaceae were enriched in non-metastatic samples,accompanied by functional shifts in pantothenate and coenzyme A biosynthesis,lysine metabolism,and lipid A pathways.Microbial network analysis further revealed differences in ecological community structure and keystone taxa:Streptococcus spp.predominated as hubs in metastatic tumors,whereas Neisseria spp.were central in non-metastatic networks.Conclusions:Overall,our findings highlight cancer-type and metastasis-specific microbial signatures,supporting a potential role for the IM in tumor progression and offering novel avenues for biomarker discovery and therapeutic targeting. 展开更多
关键词 Intratumoral microbiota lung cancer breast cancer colorectal cancer metastasis Streptococcus spp.
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Therapeutic potential of Prevotella spp. in metabolic disorders: integrating herbal medicine and gut microbiome 认领 引用
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作者 Song-Yi Han Jing-Hua Wang 《Traditional Medicine Research》 2026年第2期12-19,共8页
Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short... Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions. 展开更多
关键词 gut microbiota Prevotella spp. herbal medicine metabolic diseases microbial metabolite
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Hydroalcoholic Extracts of Achillea spp.from Greece:A Study on Phenolic Content and Their Biological Activities 认领 引用
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作者 Olga S.Tsiftsoglou Vladimir Mihailovic +4 位作者 Nikola Sreckovic Jelena S.Katanic Stankovic Kyriakos Michail Dimitriadis Michalis K.Stefanakis Diamanto Lazari 《Phyton-International Journal of Experimental Botany》 SCIE 2026年第1期34-48,共15页
Achillea species are known for their healing properties since ancient times.There is extensive literature on their pharmacological action due to their bioactive compounds.The present study aimed to investigate the ant... Achillea species are known for their healing properties since ancient times.There is extensive literature on their pharmacological action due to their bioactive compounds.The present study aimed to investigate the antioxidant and antimicrobial effects of hydroalcoholic extracts from the inflorescences and leaves of the species Achillea crithmifolia Waldst.and Kit.,A.grandifolia Friv.and A.millefolium L.The phytochemical profiles of all extracts were evaluated both by NMR spectroscopy and LC-MS analysis,and the results were consistent with the spectrophotometrically determined total phenolic(TP:125.42-191.98 mg/g)and total flavonoid(TF:47.34-180.02 mg/g)contents.All the extracts were tested for their antioxidant activity using DPPH and ABTS•+radical scavenging assay,as well as ferrous ion chelating ability and reducing power tests.All the extracts showed moderate antioxidant activity,compared to the reference substance BHT.Additionally,the antibacterial activity of the extracts was evaluated against major food-borne pathogens,showing moderate antimicrobial effects. 展开更多
关键词 Achillea spp. phenolics antioxidant activity antibacterial activity
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Network pharmacology and AI:illuminating the path to precision herbal medicine in Ganoderma spp. 认领 引用
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作者 Aman Sharma Sonali Khanal +6 位作者 Divyesh Suvedi Neelesh Yadav Rachna Verma Dinesh Kumar Ashwani Tapwal Lukas Peter Vinod Kumar 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2026年第5期559-573,共15页
Ganoderma species are promising sources of bioactive natural products with substantial pharmacological potential and have long been valued in traditional Chinese medicine(TCM)for their diverse therapeutic properties.H... Ganoderma species are promising sources of bioactive natural products with substantial pharmacological potential and have long been valued in traditional Chinese medicine(TCM)for their diverse therapeutic properties.However,their complex multi-component,multi-target nature presents significant challenges in elucidating pharmacodynamic mechanisms and optimizing clinical applications.Recent advances in network pharmacology(NP)and artificial intelligence(AI)offer innovative strategies to address these challenges.NP integrates compound-target-pathway-disease networks,while AI enhances predictive modeling,target prioritization,and the analysis of large-scale pharmacological data.Together,these approaches facilitate mechanistic interpretation,rational formulation,and personalized use of Ganodermaderived medicines.This review highlights recent progress in applying NP and AI to identify key bioactive constituents and therapeutic pathways of Ganoderma spp.,while also addressing limitations related to data quality,standardization,and clinical translation.By emphasizing the synergy between traditional TCM theory and modern computational technologies,this integrative approach advances natural medicine research and holds promise for strengthening the scientific foundation and global acceptance of Ganoderma-based therapeutics. 展开更多
关键词 Traditional Chinese Medicine Ganoderma spp. Artificial intelligence Fungi Herbal medicine Bioactive compounds Therapeutic pathways
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Methylophiopogonanone a attenuates pulmonary fibrosis by inhibiting SPP1-mediated macrophage polarization via the PI3K/Akt pathway 认领 引用
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作者 Fan Yang Tian Li +8 位作者 Weiran Jia Dingjie Xu Liangdan Sun Yanlei Ge Yiwei Shi Hong Xu Xudong Song Fuyu Jin Na Mao 《Animal Models and Experimental Medicine》 CAS CSCD 2026年第3期520-536,共17页
Background:Idiopathic pulmonary fibrosis(IPF)is an irreversible,fatal lung dis-ease.Methylophiopogonanone A(MOA),derived from the Chinese medicinal herb Ophiopogon japonicus,has been shown to exhibit anti-inflammatory... Background:Idiopathic pulmonary fibrosis(IPF)is an irreversible,fatal lung dis-ease.Methylophiopogonanone A(MOA),derived from the Chinese medicinal herb Ophiopogon japonicus,has been shown to exhibit anti-inflammatory and antioxidant properties.However,the effects of MOA on pulmonary fibrosis remain unclear.This study aims to evaluate the antifibrotic effect of MOA.Methods:The antifibrotic efficacy of MOA was evaluated in a bleomycin(BLM)-induced pulmonary fibrosis mouse model,using pirfenidone(PFD)as a positive control.Assessments included histopathology,micro-computed tomography(mi-cro-CT),lung function tests,and serum biochemistry.In vitro,RAW 264.7 murine monocyte/macrophage cells were stimulated with BLM,lipopolysaccharide(LPS),interleukin 4(IL-4),recombinant secreted phosphoprotein 1(SPP1)protein,or Spp1 overexpression(OE-spp1)and treated with MOA,PFD,spp1 shRNA(sh-spp1),or the PI3K inhibitor LY294002.Transcriptomics,molecular docking,microscale ther-mophoresis(MST),immunohistochemistry,immunofluorescence,and Western blot were used for mechanistic exploration.Results:MOA administration significantly attenuated BLM-induced lung fibrosis and collagen deposition,improved lung function,and did not induce hepatorenal toxicity.Integrated transcriptomic and bioinformatics analyses identified SPP1 as a key potential target.Molecular docking simulation and MST assays further con-firmed a favorable binding affinity between SPP1 and MOA.MOA potently inhib-ited both M1 and M2 macrophage polarization in vivo and in vitro.Mechanistically,MOA attenuated BLM-induced pulmonary fibrosis by suppressing SPP1-mediated macrophage polarization via inhibition of the PI3K/Akt pathway.Conclusions:This study identifies that MOA is a promising natural compound that al-leviates pulmonary fibrosis by inhibiting SPP1-mediated macrophage polarization via the PI3K/Akt pathway. 展开更多
关键词 macrophage polarization methylophiopogonanone A PI3K/Akt pathway pulmonary fibrosis SPP1
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Identification of Pediococcus acidilactici J1 and partial physicochemical characterization of its exopolysaccharide 认领 引用
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作者 WANG Jinlong JIANG Jing +1 位作者 YANG Ruoxi ZHAO Dan 《黑龙江大学自然科学学报》 CAS 2026年第1期85-93,共9页
Exopolysaccharides(EPS)are widely used in the food,pharmaceutical,biomaterial and other fields.Lactic acid bacteria(LAB),which are generally regarded as safe,are among the main microbial sources of EPS.In this study,a... Exopolysaccharides(EPS)are widely used in the food,pharmaceutical,biomaterial and other fields.Lactic acid bacteria(LAB),which are generally regarded as safe,are among the main microbial sources of EPS.In this study,an EPS-producing strain,designated Pediococcus acidilactici J1,was isolated and purified from a fruit fermentation broth and identified based on its morphology,API 50 CHL carbohydrate fermentation profile,16S rDNA sequence analysis,and the partial physicochemical properties of the EPS produced by strain J1(J1 EPS).The colonies of strain J1 are round,grayish-white,with smooth surfaces and neatly defined edges.A 1434 bp 16S rDNA gene sequence is obtained and shows the highest similarity to Pediococcus acidilactici 3-161 in homology analysis.The EPS yield of J1 reaches a maximum of 18.63 g·L-1 at 36 h,with a production rate of 0.52 g·L-1·h-1,which is slightly higher than that reported for other strains.In addition,J1 EPS exhibits high water solubility of(98.19±1.62)%,water-holding capacity of(406.52±6.55)%,surface hydrophobicity,good thermal stability and strong emulsifying activity.These results provide a basis for the potential application of Pediococcus acidilactici J1 EPS as a functional biopolymer in the food industry and related fields. 展开更多
关键词 Pediococcus acidilactici API identification exopolysaccharide characterization
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基于单细胞RNA转录组测序揭示CXCL9꞉SPP1极化在非酒精性脂肪性肝病进展中的驱动机制 认领 引用
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作者 鹿瑶 倪文娟 +1 位作者 陈荣翠 王玮 《中南大学学报（医学版）》 CAS CSCD 北大核心 2026年第5期924-936,共13页
目的:非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)是全球流行的慢性肝脏疾病,NAFLD的进展与Kupffer细胞(Kupffer cells,KCs)密切相关。本研究旨在分析NAFLD中KCs的C-X-C趋化因子配体9:分泌型磷蛋白1(C-X-C motif chem... 目的:非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)是全球流行的慢性肝脏疾病,NAFLD的进展与Kupffer细胞(Kupffer cells,KCs)密切相关。本研究旨在分析NAFLD中KCs的C-X-C趋化因子配体9:分泌型磷蛋白1(C-X-C motif chemokine ligand 9:secreted phosphoprotein 1,CS)极化特征及其与内皮细胞(endothelial cells,ECs)通信的机制。方法:通过高通量基因表达数据库(Gene Expression Omnibus,GEO)GSE129516数据集获取3例NAFLD小鼠和3例对照组小鼠肝脏单细胞RNA转录组测序数据,采用Seurat进行数据整合、质量控制、聚类及注释。对KCs与ECs进行亚群划分,并进行基因本体论(Gene Ontology,GO)与京都基因和基因组数据库(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析。进一步纳入小鼠肝脏单细胞数据集GSE270583对CS极化进行交叉验证。采用CellChat推断KCs亚群与ECs亚群的细胞间通信网络。将20只雄性C57BL/6J小鼠分为对照组(n=10)和高脂饮食(high-fat diet,HFD)组(n=10),每周测量小鼠体重,喂养20周时检测小鼠葡萄糖耐量和胰岛素耐量后处死小鼠,留取血清和肝脏进行后续实验。采用苏木精-伊红(hematoxylin and eosin,HE)染色和油红O染色观察肝脏病理学变化,在肝组织中通过免疫荧光染色验证CS极化与M1/M2极化特征。结果:共识别出了8类细胞的29个细胞簇。与对照组相比,NAFLD组的KCs比例升高而ECs比例降低。在M1/M2与CS极化下,解析出CD80+KCs、CD206+KCs、S100A4+KCs、分泌型磷蛋白1(secreted phosphoprotein 1,SPP1)+KCs及C-X-C趋化因子配体9(C-X-C motif chemokine ligand 9,CXCL9)+KCs等亚群,其中CD80+KCs与SPP1+KCs群体显著扩增,其特征基因主要富集于核因子-κB(nuclear factor-kappa B,NF-κB)与肿瘤坏死因子(tumor necrosis factor,TNF)信号通路。过氧化物氧化还原酶(peroxiredoxin,PRDX)3在CD80+KCs中上调,在CD206+KCs中下调。ECs可分为Apoa2+ECs、PRDX3+ECs及Col4a1+ECs 3个亚群。在NAFLD组中,PRDX3+ECs亚群异常活跃,并伴随PRDX1高表达及谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)的下调。进一步在单细胞数据集GSE270583中进行外部验证,结果显示,SPP1+KCs与CXCL9+KCs可明显分离,且NAFLD组中SPP1+KCs比例升高,支持CS极化在NAFLD中的跨队列可重复性。CellChat分析显示,来自CD80+KCs和SPP1+KCs指向PRDX3+ECs的TNF受体超家族成员1A(TNF receptor superfamily member 1A,TNFRSF1A)信号通路被激活。HFD喂养20周后,小鼠体重较对照组显著增加,并伴发葡萄糖耐量受损及胰岛素敏感性降低(均P<0.05)。HFD组的丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate transferase,AST)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)水平均显著高于对照组(均P<0.05)。HE染色和油红O染色显示,HFD组小鼠肝脏出现明显的排列紊乱、脂质空泡和脂质沉积等变化。免疫荧光染色显示,在肝组织中HFD组PRDX3、CD80表达增多;与对照组相比,HFD组肝脏KCs中SPP1表达升高,而CXCL9表达降低。这些结果与单细胞转录组测序结果一致。结论:NAFLD中KCs存在CS极化特征,SPP1+KCs和CD80+KCs比例升高,并与PRDX3+ECs之间的TNF-TNFRSF1A通信增强。HFD小鼠肝组织中SPP1、CXCL9、CD80和PRDX3表达变化与单细胞分析结果基本一致。CS极化可能参与NAFLD肝脏炎症微环境改变的机制,有望为理解NAFLD炎症-纤维化进程提供新的研究方向。 展开更多
关键词 非酒精性脂肪性肝病 单细胞RNA转录组测序 CXCL9:SPP1极化 Kupffer细胞 PRDX3
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中原名师孵化名师SPP多维培育模型的探索 认领 引用
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作者 马冬冬 《河南教育（教师教育）（下）》 2026年第4期37-38,共2页
为落实《河南省新时代中小学教师梯队攀升体系建设方案》《中原名师管理实施方案》,把中原名师工作室建设成为先进教学理念的“发射器”、教学研讨的“集散地”、青年教师成长的“助推器”、名师培养的“孵化器”,搭建教师专业提升的阶... 为落实《河南省新时代中小学教师梯队攀升体系建设方案》《中原名师管理实施方案》,把中原名师工作室建设成为先进教学理念的“发射器”、教学研讨的“集散地”、青年教师成长的“助推器”、名师培养的“孵化器”,搭建教师专业提升的阶梯与支架,中原名师马冬冬综合实践工作室经过几年的探索,构建了中原名师孵化名师SPP多维培育模型。一、SPP多维培育模型解读(一)理论基础SPP模型是基于“知行合一”的教育理论和陈鹤琴先生“活教育”教育思想建构的一种对话研修模型。 展开更多
关键词 中原名师 SPP多维培育模型 教师梯队攀升体系建设
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SPP1在T1期结直肠癌中的表达与淋巴结转移 之间的关系 认领 引用
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作者 裴佳欣 王世钰 +2 位作者 刘慧敏 尹玉 蔡永萍 《临床医学进展》 2026年第3期1058-1068,共11页
目的:探讨分泌性磷蛋白1 (Secreted Phosphoprotein 1, SPP1)在T1期结直肠癌中的表达与淋巴结转移之间的关系。方法:在209例T1期结直肠癌和10例T2~T3期微乳头型结直肠癌石蜡包埋组织中通过免疫组织化学方法检测SPP1表达并分析其表达与... 目的:探讨分泌性磷蛋白1 (Secreted Phosphoprotein 1, SPP1)在T1期结直肠癌中的表达与淋巴结转移之间的关系。方法:在209例T1期结直肠癌和10例T2~T3期微乳头型结直肠癌石蜡包埋组织中通过免疫组织化学方法检测SPP1表达并分析其表达与淋巴结转移以及其他病理特征之间的关系。结果:1、与高级别上皮内瘤变相比,SPP1在腺癌间质中的表达显著升高(P < 0.001),且在肿瘤浸润前沿的表达高于肿瘤中央(P < 0.001)。2、肿瘤浸润前沿与肿瘤中央SPP1高表达与肿瘤出芽、低分化肿瘤细胞簇的分级呈正相关。3、与非特殊类型腺癌相比,微乳头型腺癌中观察到SPP1的表达升高(P < 0.05)。4、肿瘤浸润前沿与肿瘤中央SPP1高表达均与脉管侵犯呈正相关(P < 0.05)。5、Logistic多因素回归分析结果显示,肿瘤浸润前沿SPP1高表达和脉管侵犯均是发生淋巴结转移的独立预测因素(P < 0.05)。结论:肿瘤浸润前沿SPP1高表达是T1期结直肠癌发生淋巴结转移的独立预测因素,提示可能用于识别具有淋巴结转移风险的T1期结直肠癌患者,有助于指导临床治疗方案的选择。 展开更多
关键词 T1期结直肠癌 SPP1 淋巴结转移 肿瘤出芽 低分化肿瘤细胞簇 微乳头型腺癌
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SPP1在肺腺癌中的表达及与M2型巨噬细胞 浸润的关系 认领 引用
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作者 刘梦玉 刘百龙 《临床医学进展》 2026年第5期2127-2140,共14页
目的:探讨分泌型磷蛋白1 (SPP1)在肺腺癌中的表达及其与M2型巨噬细胞浸润的关系。方法:基于TCGA数据库及GEPIA、TIMER等平台分析SPP1在肺腺癌中的表达、生存相关性及免疫浸润特征;收集50例肺腺癌及对应癌旁组织,采用免疫组织化学和免疫... 目的:探讨分泌型磷蛋白1 (SPP1)在肺腺癌中的表达及其与M2型巨噬细胞浸润的关系。方法:基于TCGA数据库及GEPIA、TIMER等平台分析SPP1在肺腺癌中的表达、生存相关性及免疫浸润特征;收集50例肺腺癌及对应癌旁组织,采用免疫组织化学和免疫荧光检测SPP1及CD206表达,并分析其临床意义。结果:生物信息学分析显示,SPP1在肺腺癌组织中高表达,并与较差总体生存及髓系免疫细胞浸润增加相关。临床样本验证显示,SPP1在肺腺癌组织中的高表达率高于癌旁组织,并与TNM分期及疾病进展相关。SPP1高表达组无进展生存期呈缩短趋势,但差异未达统计学意义,且多因素Cox分析未提示其为独立预后因素。免疫荧光结果显示,SPP1表达与CD206+ M2型巨噬细胞密度呈正相关。结论:SPP1在肺腺癌中高表达,并与肿瘤进展及M2型巨噬细胞浸润增加相关,可能参与肺腺癌免疫微环境重塑,但其独立预后价值仍需进一步验证。 展开更多
关键词 肺腺癌 SPP1 肿瘤相关巨噬细胞 预后 肿瘤微环境
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The Application Value of Chest CT Combined with Serum Vanin-1 and SPP1 in Diagnosing the Severity of Chronic Obstructive Pulmonary Disease 认领 引用
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作者 Yufei Wei Yijie Cui +1 位作者 Wei Zhang Xueyao Wang 《Journal of Clinical and Nursing Research》 2026年第1期354-359,共6页
Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100... Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100 COPD subjects from our hospital from January 2020 to December 2023 were included and randomly divided into a healthy control group and an experimental group(50 cases each).The healthy control group underwent slow vital capacity measurement using a spirometer,while the experimental group underwent high-resolution thin-slice CT scans and serum Vanin-1 and SPP1 concentration measurements.Pulmonary function parameters,symptom burden,biomarker concentrations,and imaging characteristics were compared between the two groups.Results:The FEV1/FVC ratio in the experimental group(58.3±7.2)was lower than that in the healthy control group(92.1±4.8);the total CAT score(22.4±3.5)was higher than that in the healthy control group(3.1±1.2);both Vanin-1(18.7±2.3μg/L)and SPP1(25.6±4.1μg/L)levels were higher than those in the healthy control group;LAA%-950(38.7±6.2%)and WA%(68.5±5.3%)were significantly higher than those in the healthy control group(all p<0.001).Conclusion:Chest CT combined with serum Vanin-1 and SPP1 can accurately quantify the pathological progression of COPD,providing a dual basis for clinical staging and individualized intervention. 展开更多
关键词 Chronic obstructive pulmonary disease Chest CT Vanin-1 SPP1 Disease assessment
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SMARCA4缺失型非小细胞肺癌的SPP1表达及其与PD-L1的关系 认领 引用 被引量：3
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作者 吴娟 黄曦 +5 位作者 李佳嘉 魏雨晴 张丽琴 俞咏梅 陆志伟 张鹤 《中国临床药理学与治疗学》 CAS CSCD 北大核心 2025年第4期477-486,共10页
目的:分析SMARCA4缺失型非小细胞肺癌中分泌性磷蛋白1(SPP1)及细胞程序性死亡-配体1(PD-L1)的表达,为罕见病理类型肺癌后续治疗研究提供科学依据。方法:回顾性分析12例SMARCA4缺失型非小细胞肺癌患者的临床与病理特征,根据其形态学分为... 目的:分析SMARCA4缺失型非小细胞肺癌中分泌性磷蛋白1(SPP1)及细胞程序性死亡-配体1(PD-L1)的表达,为罕见病理类型肺癌后续治疗研究提供科学依据。方法:回顾性分析12例SMARCA4缺失型非小细胞肺癌患者的临床与病理特征,根据其形态学分为腺癌与低分化癌两组,分析SPP1与PD-L1在两组的表达与关系。结果:所有患者均检出SPP1表达且其在低分化癌组表达水平较腺癌组显著升高(P=0.015);PD-L1表达者占6/7(5例未测),较之腺癌组,PD-L1亦在低分化癌组高表达(P=0.048),两组的PD-L1表达差异与SPP1表达差异一致。结论:SMARCA4缺失型非小细胞肺癌具有较高的SPP1及PD-L1阳性表达,病理形态为低分化癌者更甚,SPP1与PD-L1在SMARCA4缺失型非小细胞肺癌中的表达情况可能具有正相关性,其关联机制及免疫治疗中作用有待后续研究进一步探讨。 展开更多
关键词 NSCLC SMARCA4缺失 SPP1 PD-L1 免疫治疗
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Desiccation and osmotic resistance mechanism of Cronobacter spp.isolated from powdered infant formula 认领 引用 被引量：1
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作者 Shiqian Fu Danliangmin Song +5 位作者 Xue Qin Lihan Wang Qianyu Zhao Chaoxin Man Xinyan Yang Yujun Jiang 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第3期968-980,共13页
Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter... Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time. 展开更多
关键词 Powdered infant formula Cronobacter spp. Desiccation resistance Osmotic resistance Transcriptomics
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龙胆苦苷通过调节肝星状细胞中MIF-SPP1信号通路预防巨噬细胞介导的肝纤维化机制研究 认领 引用 被引量：2
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作者 王继绪 朱英斌 +1 位作者 陈茂丽 韩永峰 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2025年第7期593-602,共10页
目的探究龙胆苦苷(GPS)通过调节肝星状细胞中巨噬细胞迁移抑制因子(MIF)-分泌型磷蛋白1(SPP1)信号通路预防巨噬细胞介导的肝纤维化机制。方法将LX-2细胞分为对照组、转化生长因子β(TGF-β)组、TGF-β联合GPS(25、50、100、150)μmol/mL... 目的探究龙胆苦苷(GPS)通过调节肝星状细胞中巨噬细胞迁移抑制因子(MIF)-分泌型磷蛋白1(SPP1)信号通路预防巨噬细胞介导的肝纤维化机制。方法将LX-2细胞分为对照组、转化生长因子β(TGF-β)组、TGF-β联合GPS(25、50、100、150)μmol/mL组,EDU检测细胞增殖、Transwell TM检测细胞侵袭、Western blot法检测平滑肌激动蛋白(α-SMA)与一型胶原蛋白(COL1A1)蛋白表达。分离M1型巨噬细胞条件培养基(M1-CM)用于处理TGF-β组、TGF-β联合GPS组LX-2细胞,同时检测细胞上清液中诱导型一氧化氮合酶(iNOS)、精氨酸酶1(Arg1)浓度,细胞增殖与侵袭能力,以及α-SMA与COL1A1蛋白表达。生物信息学分析GPS、肝纤维化与巨噬细胞相关基因的靶点交集,药物亲和反应的靶点稳定性(DARTS)实验和Western blot法验证GPS对MIF的调控作用。进一步将LX-2细胞分为对照组、TGF-β组、TGF-β联合M2-CM组、TGF-β和oe-NC联合M2-CM组、TGF-β和oe-MIF联合M2-CM组,分析细胞上清液iNOS、Arg1浓度及细胞增殖、侵袭、α-SMA与COL1A1蛋白表达变化。将LX-2细胞分为对照组、TGF-β组、TGF-β联合oe-NC组、TGF-β联合oe-MIF组、TGF-β和oe-MIF联合GPS组,Western blot法测定MIF与SPP1蛋白表达。构建大鼠肝纤维化模型,探究GPS对体内肝纤维化的潜在治疗作用。结果与对照组相比,TGF-β组LX-2细胞增殖与侵袭能力增加,α-SMA与COL1A1蛋白表达增强,而GPS干预能够抑制TGF-β条件LX-2细胞增殖与侵袭,并降低α-SMA与COL1A1蛋白表达。与对照组相比,TGF-β组细胞上清液中iNOS浓度上调、Arg1浓度下降,并且M1-CM处理在TGF-β干预的基础上,进一步增加了iNOS浓度、降低了Arg1浓度,同时促进了细胞增殖与侵袭,上调了α-SMA与COL1A1蛋白表达,而GPS能够逆转M1-CM干预的结果。生物信息学分析发现MIF为GPS、肝纤维化与巨噬细胞相关基因的靶点交集之一,且GPS能够靶向并抑制其表达。相比于TGF-β组,M2-CM干预后细胞上清液中iNOS浓度下降、Arg1浓度增加,LX-2细胞增殖与侵袭能力降低,α-SMA与COL1A1蛋白表达减弱,然而过表达MIF后,逆转了M2-CM的干预效果。Western blot结果显示,相比于对照组,TGF-β组MIF与SPP1蛋白表达增强,过表达MIF后MIF与SPP1蛋白表达进一步增强,而GPS干预则抑制了MIF与SPP1蛋白表达。动物实验中,GPS干预治疗能够减轻肝纤维化大鼠肝损伤,并抑制肝组织中MIF与SPP1、α-SMA与COL1A1蛋白表达。结论GPS可能通过抑制肝星状细胞中MIF-SPP1信号通路预防巨噬细胞介导的肝纤维化。 展开更多
关键词 巨噬细胞 龙胆苦苷(GPS) 肝星状细胞 肝纤维化 巨噬细胞迁移抑制因子(MIF) 分泌型磷蛋白1(SPP1)
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Regulation of ethyl carbamate by BTN2 of Saccharomyces cerevisiae during mixed-culture Huangjiu fermentation with Pediococcus pentoses 认领 引用
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作者 Ruosi Fang Huqi Zhou +8 位作者 Chuanchuan Shi Tangchao Chen Jingjin Hu Wenjing Lin Xueqi Tang Hanwen Xu Anan Zhou Zhongxiang Fang Gongnian Xiao 《Food Innovation and Advances》 2025年第4期480-489,共10页
Ethyl carbamate(EC)is a naturally occurring potential carcinogen in fermented foods.Our prior research indicated that BTN2 knockout affected arginine metabolism in Saccharomyces cerevisiae,and the inhibitory effect wa... Ethyl carbamate(EC)is a naturally occurring potential carcinogen in fermented foods.Our prior research indicated that BTN2 knockout affected arginine metabolism in Saccharomyces cerevisiae,and the inhibitory effect was more pronounced in mixed cultures with Pediococcus pentoses(PP).Consequently,in this study,we investigated the potential mechanisms of EC regulation by BTN2 knockout strains in single-and mixed-culture fermentation systems in Huangjiu and determined their fundamental qualities.The findings revealed that the BTN2 knockout strain could reduce the EC content by decreasing the amount of EC precursors and that mixed fermentation with PP could impede the reaction between urea and ethanol,thereby exerting a more favorable EC abatement effect.The BTN2 knockout strain had a positive effect on free amino acids in Huangjiu,thus improving the flavor.Additionally,transcriptome analysis demonstrated that the knockout of BTN2 and the addition of PP affected gene expression levels,paticularly genes associated with transcription factor activity and amino acid transport in S.cerevisiae,which subsquently affected the metabolic pathways during the fermentation process. 展开更多
关键词 fermented foodsour saccharomyces cerevisiaeand pediococcus pentoses pp consequentlyin ethyl carbamate ec mixed cultures btn knockout arginine metabolism ethanol
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From bulb development to postharvest treatments:advances in Hippeastrum spp.research and industry applications 认领 引用
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作者 Lingmei Shao Liuyan Yang +3 位作者 Xin Li Lin Zhou Jiao Zhu Yongchun Zhang 《Ornamental Plant Research》 2025年第1期193-202,共10页
Hippeastrum spp.,renowned as highly sought-after perennial bulbous flowers,have gained increasing popularity in landscape gardening and festive markets due to their unique ornamental value.However,the high cost of bul... Hippeastrum spp.,renowned as highly sought-after perennial bulbous flowers,have gained increasing popularity in landscape gardening and festive markets due to their unique ornamental value.However,the high cost of bulbs and the lack of advanced techniques for regulating bulb development pose significant challenges to their industrialization.This review synthesizes recent progress in the study of Hippeastrum bulb morphology,developmental regulatory mechanisms,and postharvest treatments.It highlights the effects of factors such as light,temperature,nutrients,and phytohormones on bulb growth while also summarizing cultivation techniques and strategies for regulating bulb development.Based on current research,regulating bulb development emerges as a pivotal approach for improving bulb quality,reducing production costs,and enhancing both ornamental and medicinal potential.This review aims to provide a theoretical foundation for advancing the understanding of Hippeastrum bulb development and promoting its industrial-scale production,thereby fostering further research in this field. 展开更多
关键词 bulb development hippeastrum spp regulatory mechanisms medicinal potential ornamental value landscape gardening postharvest treatments cultivation techniques
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BDSBAS和WAAS对单频SPP精度增强对比分析 认领 引用
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作者 陈红英 陈韵陶 《全球定位系统》 CSCD 2025年第3期82-87,共6页
星基增强系统(Satellite-Based Augmentation System,SBAS)在民航等精密定位领域的重要性逐渐凸显,但目前尚缺乏针对北斗星基增强系统(BeiDou Satellite-Based Augmentation System,BDSBAS)和广域增强系统(Wide Area Augmentation Syste... 星基增强系统(Satellite-Based Augmentation System,SBAS)在民航等精密定位领域的重要性逐渐凸显,但目前尚缺乏针对北斗星基增强系统(BeiDou Satellite-Based Augmentation System,BDSBAS)和广域增强系统(Wide Area Augmentation System,WAAS)在单频单点定位(single point positioning,SPP)应用中的性能差异对比,因此本文深入分析了SBAS对单频定位的精度增强.文章通过使用中国境内和北美地区的国际GNSS服务组织(International GNSS Service,IGS)多系统GNSS实验(Multi-GNSS Experiment,MGEX)数据,对比分析了BDSBAS和WAAS地球静止轨道(geostationary orbit,GEO)卫星的单频SPP定位效果,探讨了不同增强策略对轨道、钟差和电离层延迟的改正,验证了SBAS对定位精度的提升效果.结果显示,在各测站中,WAAS的表现优于BDSBAS,对均方根(root mean square,RMS)的最大改善达到60%,而BDSBAS在中国及周边地区也具有良好的适应性,对RMS的最大改善能达到50%,结合轨道、钟差和电离层延迟改正的策略,特别是SF_SBAS_ORB_ION在定位精度上表现最佳,在电离层影响显著的条件下,显著减少了误差并提高了定位稳定性.文章为SBAS在单频精密定位中的应用提供了新的数据支撑和参考. 展开更多
关键词 星基增强系统(SBAS) 定位增强 单频单点定位(SPP) 轨道误差 电离层延迟
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The Predictive Value of SPP1 Gene Expression for the Survival of Advanced Liver Cancer Treated with Transarterial Chemoembolization 认领 引用
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作者 Yu Cai Pu Yan +3 位作者 Chang Tian Yuqing Li Yuanyuan Jia Siqi Wang 《Proceedings of Anticancer Research》 2025年第1期97-107,共11页
Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization... Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization treatment.Method:Bioinformatics methods,including gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,were used to identify genes related to survival prognosis in hepatocellular carcinoma(HCC)patients.A retrospective analysis of 115 advanced liver cancer patients treated between January 2016 and October 2017 was conducted.Patients were categorized into SPP1 high-expression(n=89)and low-expression groups(n=26).Additionally,115 healthy individuals served as the control group.The relationship between SPP1 expression and clinical pathological features was analyzed.A 60-month follow-up and logistic regression analysis identified risk factors affecting survival.Results:SPP1 mRNA expression was significantly higher in liver cancer patients compared to healthy controls(P<0.05).SPP1 expression levels were significantly associated with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging(P<0.05).High SPP1 expression,along with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,were independent risk factors for survival(P<0.05).The 60-month survival rate was 17.39%,with a median survival of 40 months in the low-expression group versus 18 months in the high-expression group(P<0.05).Conclusion:SPP1 expression is significantly upregulated in advanced liver cancer patients and has predictive value for postoperative survival following hepatic artery chemoembolization treatment.SPP1,combined with clinical indicators such as tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,may serve as a prognostic biomarker for interventional treatment outcomes. 展开更多
关键词 SPP1 Transarterial chemoembolization Advanced liver cancer Survival period Predictive value
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