Gastric cancer(GC)is characterized by high morbidity and mortality rates.Chinese agarwood comprises the resin-containing wood of Aquilaria sinensis(Lour.)Gilg.,traditionally utilized for treating asthma,cardiac ischem...Gastric cancer(GC)is characterized by high morbidity and mortality rates.Chinese agarwood comprises the resin-containing wood of Aquilaria sinensis(Lour.)Gilg.,traditionally utilized for treating asthma,cardiac ischemia,and tumors.However,comprehensive research regarding its anti-GC effects and underlying mechanisms remains limited.In this study,Chinese agarwood petroleum ether extract(CAPEE)demonstrated potent cytotoxicity against human GC cells,with half maximal inhibitory concentration(IC50)values for AGS,HGC27,and MGC803 cells of 2.89,2.46,and 2.37μg·mL−1,respectively,at 48 h.CAPEE significantly induced apoptosis in these GC cells,with B-cell lymphoma-2(BCL-2)associated X protein(BAX)/BCL-2 antagonist killer 1(BAK)likely mediating CAPEE-induced apoptosis.Furthermore,CAPEE induced G0/G1phase cell cycle arrest in human GC cells via activation of the deoxyribonucleic acid(DNA)damage-p21-cyclin D1/cyclin-dependent kinase 4(CDK4)signaling axis,and increased Fe2+,lipid peroxides and reactive oxygen species(ROS)levels,thereby inducing ferroptosis.Ribonucleic acid(RNA)sequencing,real-time quantitative polymerase chain reaction(RT-qPCR),and Western blotting analyses revealed CAPEE-mediated upregulation of heme oxygenase-1(HO-1)in human GC cells.RNA interference studies demonstrated that HO-1 knockdown reduced CAPEE sensitivity and inhibited CAPEE-induced ferroptosis in human GC cells.Additionally,CAPEE administration exhibited robust in vivo anti-GC activity without significant toxicity in nude mice while inhibiting tumor cell growth and promoting apoptosis in tumor tissues.These findings indicate that CAPEE suppresses human GC cell growth through upregulation of the DNA damage-p21-cyclin D1/CDK4 signaling axis and HO-1-mediated ferroptosis,suggesting its potential as a candidate drug for GC treatment.展开更多
By growth rate method, the inhibitory effects of five solvent extracts from Helianthus tuberosus leaves against Phytophthora capsici were studied in the test. The results showed that different solvent extracts all had...By growth rate method, the inhibitory effects of five solvent extracts from Helianthus tuberosus leaves against Phytophthora capsici were studied in the test. The results showed that different solvent extracts all had inhibitory effect against P. capsici, while 12.5 mg/mL of extracts from H. tuberosus leaves with petroleum ether and ethyl acetate as solvents had the highest inhibitory effect against P. capsici, reaching 100%. In case of various solvent extracts with different concentration gradients, ethyl acetate extract had the most significant inhibitory effect; when the concentration was 5 mg/mL, the inhibitory effect of ethyl acetate extract had reached 100% ; when the concentration reduced to 2.5 mg/mL, the inhibitory effect was still (27.91 ±2. 076) %, significantly higher than that of other solvent extracts at the same concentration. The 50 times dilution of ethyl acetate extract from H. tuberosus leaves was selected for pot test against pepper blight. , and the results showed that its control effect against pepper blight reached 100.00%, superior than that of chemical agent 25% metalaxyl WP 400 times dilution.展开更多
[Objective] This study aimed to investigate the effects of different irrigation modes on chemical constituent and aroma substance contents and sensory quality in flue-cured tobacco leaves. [Method] Field experiments w...[Objective] This study aimed to investigate the effects of different irrigation modes on chemical constituent and aroma substance contents and sensory quality in flue-cured tobacco leaves. [Method] Field experiments were conduced with two treatments, including watering irrigation and micro-spraying irrigation. In watering irrigation treatment, flue-cured tobacco seedlings were respectively watered by artificial root irrigation once at rosette stage(May 26) and vigorous growth stage(June 18), 1kg/plant each time; in micro-spraying treatment, flue-cured tobacco seedlings were watered by artificial root irrigation once at rosette stage(May 26), 1 L/plant. [Result]Compared to watering irrigation mode, micro-spraying irrigation could extremely significantly increase the content of main aromatic substance in middle and upper leaves.Contents of main aromatic substance non-volatile organic acids, volatile organic acids, petroleum ether extracts, neophytadiene and neutral aromatic substances in C3F tobacco leaves were improved by 24.4%, 32.6%, 20.7%, 33.8% and 26.3%, respectively; contents of main aromatic substance non-volatile organic acids, volatile organic acids, petroleum ether extracts, eophytadiene and neutral aromatic substances in B2F tobacco leaves were improved by 16.3%, 6.0%, 6.1%, 10.8% and8.1%, respectively. In addition, micro-spraying irrigation could extremely significantly reduce the nicotine content in middle and upper leaves and improve the aromatic coordination, mellow taste and smoldering property. [Conclusion] In flue-cured tobacco production, micro-spraying technology is an important means to improve the internal quality of flue-cured tobacco leaves, which can be adopted to effectively solve the problem of declining quality of flue-cured tobacco leaves resulted from the dry climate at maturation stage in seasonal frequent-drought areas.展开更多
基金supported by the Natural Science Foundation of Beijing City(No.J230034)the Fundamental Research Funds for the Central Universities(No.2023-JYB-JBQN-051)the Talent Cultivation Project of Beijing University of Chinese Medicine(No.JZPY202206).
摘要Gastric cancer(GC)is characterized by high morbidity and mortality rates.Chinese agarwood comprises the resin-containing wood of Aquilaria sinensis(Lour.)Gilg.,traditionally utilized for treating asthma,cardiac ischemia,and tumors.However,comprehensive research regarding its anti-GC effects and underlying mechanisms remains limited.In this study,Chinese agarwood petroleum ether extract(CAPEE)demonstrated potent cytotoxicity against human GC cells,with half maximal inhibitory concentration(IC50)values for AGS,HGC27,and MGC803 cells of 2.89,2.46,and 2.37μg·mL−1,respectively,at 48 h.CAPEE significantly induced apoptosis in these GC cells,with B-cell lymphoma-2(BCL-2)associated X protein(BAX)/BCL-2 antagonist killer 1(BAK)likely mediating CAPEE-induced apoptosis.Furthermore,CAPEE induced G0/G1phase cell cycle arrest in human GC cells via activation of the deoxyribonucleic acid(DNA)damage-p21-cyclin D1/cyclin-dependent kinase 4(CDK4)signaling axis,and increased Fe2+,lipid peroxides and reactive oxygen species(ROS)levels,thereby inducing ferroptosis.Ribonucleic acid(RNA)sequencing,real-time quantitative polymerase chain reaction(RT-qPCR),and Western blotting analyses revealed CAPEE-mediated upregulation of heme oxygenase-1(HO-1)in human GC cells.RNA interference studies demonstrated that HO-1 knockdown reduced CAPEE sensitivity and inhibited CAPEE-induced ferroptosis in human GC cells.Additionally,CAPEE administration exhibited robust in vivo anti-GC activity without significant toxicity in nude mice while inhibiting tumor cell growth and promoting apoptosis in tumor tissues.These findings indicate that CAPEE suppresses human GC cell growth through upregulation of the DNA damage-p21-cyclin D1/CDK4 signaling axis and HO-1-mediated ferroptosis,suggesting its potential as a candidate drug for GC treatment.
基金Supported by Applied Basic Research Project of Qinghai Provincial Science and Technology Department(2014-ZJ-735)
摘要By growth rate method, the inhibitory effects of five solvent extracts from Helianthus tuberosus leaves against Phytophthora capsici were studied in the test. The results showed that different solvent extracts all had inhibitory effect against P. capsici, while 12.5 mg/mL of extracts from H. tuberosus leaves with petroleum ether and ethyl acetate as solvents had the highest inhibitory effect against P. capsici, reaching 100%. In case of various solvent extracts with different concentration gradients, ethyl acetate extract had the most significant inhibitory effect; when the concentration was 5 mg/mL, the inhibitory effect of ethyl acetate extract had reached 100% ; when the concentration reduced to 2.5 mg/mL, the inhibitory effect was still (27.91 ±2. 076) %, significantly higher than that of other solvent extracts at the same concentration. The 50 times dilution of ethyl acetate extract from H. tuberosus leaves was selected for pot test against pepper blight. , and the results showed that its control effect against pepper blight reached 100.00%, superior than that of chemical agent 25% metalaxyl WP 400 times dilution.
基金Supported by Science and Technology Project of Yunnan Zhongyan Industry Co.,Ltd.(2010YL01-2)Science and Technology Project of Hongyun Honghe Tobacco(Group) Co.Ltd.(HYHH2012YL01)~~
摘要[Objective] This study aimed to investigate the effects of different irrigation modes on chemical constituent and aroma substance contents and sensory quality in flue-cured tobacco leaves. [Method] Field experiments were conduced with two treatments, including watering irrigation and micro-spraying irrigation. In watering irrigation treatment, flue-cured tobacco seedlings were respectively watered by artificial root irrigation once at rosette stage(May 26) and vigorous growth stage(June 18), 1kg/plant each time; in micro-spraying treatment, flue-cured tobacco seedlings were watered by artificial root irrigation once at rosette stage(May 26), 1 L/plant. [Result]Compared to watering irrigation mode, micro-spraying irrigation could extremely significantly increase the content of main aromatic substance in middle and upper leaves.Contents of main aromatic substance non-volatile organic acids, volatile organic acids, petroleum ether extracts, neophytadiene and neutral aromatic substances in C3F tobacco leaves were improved by 24.4%, 32.6%, 20.7%, 33.8% and 26.3%, respectively; contents of main aromatic substance non-volatile organic acids, volatile organic acids, petroleum ether extracts, eophytadiene and neutral aromatic substances in B2F tobacco leaves were improved by 16.3%, 6.0%, 6.1%, 10.8% and8.1%, respectively. In addition, micro-spraying irrigation could extremely significantly reduce the nicotine content in middle and upper leaves and improve the aromatic coordination, mellow taste and smoldering property. [Conclusion] In flue-cured tobacco production, micro-spraying technology is an important means to improve the internal quality of flue-cured tobacco leaves, which can be adopted to effectively solve the problem of declining quality of flue-cured tobacco leaves resulted from the dry climate at maturation stage in seasonal frequent-drought areas.