Pinus radiata(D.Don)dominates New Zealand's forestry industry,constituting 91%of plantations,and is among the world's most important plantation species.Given the socio-economic and environmental importance of ...Pinus radiata(D.Don)dominates New Zealand's forestry industry,constituting 91%of plantations,and is among the world's most important plantation species.Given the socio-economic and environmental importance of this species,it is important to have accurate and precise projections over time to make efficient decisions for forest management and greenfield investments in afforestation projects,especially for permanent carbon forests.Future projections of any natural resource systems rely on modeling;however,the acceleration of climate change makes future projections of yield less certain.These challenges also impact national expectations of the contribution planted forests will provide to address climate change and meet international commitments under the Paris Agreement.Using a large national-scale set of contemporary ground-measured data(2013–2023),this study investigates the performance of two growth models developed over 30 years ago that are widely used by NZ plantation growers:1)the Pumice Plateau Model 1988(PPM88)and 2)the 300-index(including a model variant of regional drift).Model simulations were made using the FORECASTER modeling suite with geographic boundaries to adjust for drift in space and time.Basal area(BA,m2⋅ha-1)and volume(m3⋅ha-1)were simulated,and standard errors and goodness-of-fit metrics calculated up to a typical rotation age of 30 years.Model residuals were then separated and analysed for the main plantation growing regions.The models overpredicted observed growth by between 6.8%and 16.2%,but model predictions and errors varied significantly between regions.The results of this study provided clear evidence of divergence between the outputs of both models and the measured data.Finally,this study suggests future measures to address challenges posed by these discrepancies that will provide better information for forest management and investment decisions in a changing climate.展开更多
Plant functional traits are key for understanding the adaptive strategies to environments,and fine roots play a crucial role in nutrient acquisition.Examining the variation of functional traits of fine root and soil p...Plant functional traits are key for understanding the adaptive strategies to environments,and fine roots play a crucial role in nutrient acquisition.Examining the variation of functional traits of fine root and soil physicochemical properties,and investigating their coupling relationships and dominating factors,could provide a theoretical foundation for ecological restoration in the burned forest.We established 12 plots within Pinus tabuliformis Carrière forests subjected to light,moderate and severe fire severities.Through detailed analysis of fine roots and soil physicochemical properties,we evaluated the variations and coupling effects in fine root functional traits and soil properties using the Coupling Coordination Degree Model and Partial Least Squares Path Modeling.Our results showed significant differences in the functional traits of fine root and soil physicochemical properties across fire severities(P<0.05).The coupling coordination degrees between fine root functional features and soil physicochemical properties ranged from 0.4 to 0.6,with the following order:unburned,moderate,severe and light severity.Forest fire negatively impacted the coupling coordination degree indirectly,primarily influenced by the direct positive effects of fine root morphological traits(e.g.,specific root length)and soil nutrient properties(e.g.,nitrogen and available phosphorus).The synergistic recovery of fine root-soil systems in Pinus tabuliformis forests was most pronounced following moderate fire severity,showing a medium-level coordination degree.For light-severity fires,enhancing fine root morphological characteristics through soil warming is recommended.In contrast,it is suggested to apply appropriate nitrogen and soil fertilizers for improving soil conditions after severe fire.展开更多
Global change drivers,including drought and nitrogen(N)deposition,exert a wide-ranging influence on tree growth and fitness.However,our current understanding of their combined effects is still limited.Non-structural c...Global change drivers,including drought and nitrogen(N)deposition,exert a wide-ranging influence on tree growth and fitness.However,our current understanding of their combined effects is still limited.Non-structural carbohydrate(NSC)storage is an important physiological trait for tree acclimation to drought.It acts as an important mobile carbon reserve to support tree function when carbon fixation or transport are reduced under drought.It is crucial to investigate how tree species with different NSC storage characteristics(e.g.,storage level,partitioning)respond to drought events,and how N alters these patterns.We investigated the combined effects of drought(80%reduction in precipitation)and N addition(0,30,and 120 kg/ha/year)on the growth and NSC storage of Pinus koraiensis and Fraxinus mandshurica(dominant species in the forests of Northeast China)saplings over two consecutive growing seasons.The results indicated that P.koraiensis exhibited high tolerance to drought,with growth unaffected by drought alone until the mid-growing season in the second year.However,N addition reversed its drought acclimation by impairing root development and exacerbating carbon shortage.In contrast,F.mandshurica was sensitive to drought,it had significantly reduced growth at harvest despite a large amount of NSC accumulation.The present study highlights the contrasting effects of N deposition on drought adaptation in coexisting conifer and temperate broadleaf species,the conifer showing a higher risk of carbon deficiency with increasing N deposition(i.e.,a stronger reversal effect of N addition),whereas an earlier cessation of growth under drought defines a larger carbon safety margin for broadleaved species.These results have important implications for the development of adaptive forest management strategies such as to enhance the protection of conifers in the context of global change.展开更多
The level of genetic variation within a breeding population affects the effectiveness of selection strategies for genetic improvement.The relationship between genetic variation level within Pinus tabuliformis breeding...The level of genetic variation within a breeding population affects the effectiveness of selection strategies for genetic improvement.The relationship between genetic variation level within Pinus tabuliformis breeding populations and selection strategies or selection effectiveness is not fully investigated.Here,we compared the selection effectiveness of combined and individual direct selection strategies using half-and full-sib families produced from advanced-generation P.tabuliformis seed orchard as our test populations.Our results revealed that,within half-sib families,average diameter at breast height(DBH),tree height,and volume growth of superior individuals selected by the direct selection strategy were higher by 7.72%,18.56%,and 31.01%,respectively,than those selected by the combined selection strategy.Furthermore,significant differences(P<0.01)were observed between the two strategies in terms of the expected genetic gains for average tree height and volume.In contrast,within full-sib families,the differences in tree average DBH,height,and volume between the two selection strategies were relatively minor with increase of 0.17%,2.73%,and 2.21%,respectively,and no significant differences were found in the average expected genetic gains for the studied traits.Half-sib families exhibited greater phenotypic and genetic variation,resulting in improved selection efficiency with the direct selection strategy but also introduced a level of inbreeding risk.Based on genetic distance estimates using molecular markers,our comparative seed orchard design analysis showed that the Improved Adaptive Genetic Programming Algorithm(IAPGA)reduced the average inbreeding coefficient by 14.36% and 14.73% compared to sequential and random designs,respectively.In conclusion,the combination of the direct selection strategy with IAPGA seed orchard design aimed at minimizing inbreeding offered an efficient approach for establishing advanced-generation P.tabuliformis seed orchards.展开更多
The defense mechanisms induced in wild Chinese pine(Pinus tabuliformis)in response to herbivores are not well characterized,especially in the field.To address this knowledge gap,we established a biological model syste...The defense mechanisms induced in wild Chinese pine(Pinus tabuliformis)in response to herbivores are not well characterized,especially in the field.To address this knowledge gap,we established a biological model system to evaluate proteome variations in pine needles after feeding by the Chinese pine caterpillar(Dendrolimus tabulaeformis),a major natural enemy and dominant herbivore.Quantitative tandem mass tag(TMT)proteomics and bioinformatics were utilized to systematically identify differentially abundant proteins implicated in the induced defense response of Chinese pine.We validated key protein changes using parallel reaction monitoring(PRM)technology.Pathway analysis revealed that the induced defenses involved phenylpropanoid,coumarin,and flavonoid biosynthesis,among other processes.To elucidate the regulatory patterns underlying pine resistance,we determined the activities of defense enzymes and levels of physiological and biochemical compounds.In addition,the expression of upstream genes for key proteins was validated by qRT-PCR.Our results provide new molecular insights into the induced defense mechanisms in Chinese pine against this caterpillar in the field.A better understanding of these defense strategies will inform efforts to breed more-resistant pine varieties.展开更多
Introduction Petrochemical feedstocks have been influential in pro-viding modern luxuries such as chemicals,energy,and materials.However,issues such as waste accumulation,environmental pollution,climate change,and res...Introduction Petrochemical feedstocks have been influential in pro-viding modern luxuries such as chemicals,energy,and materials.However,issues such as waste accumulation,environmental pollution,climate change,and resource depletion are common side-effects of this technology.With a growing population and its associated demand,there is an urgent need to switch to a cleaner means of production,whilst maintaining similar or better benefits than existing technology(Feng et al.2013;Neiva et al.2018,2020).展开更多
Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China,known as the mixed broadleaf-Korean pine forest.Although carbohydrate metabolism pathways in the seed embryo are kno...Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China,known as the mixed broadleaf-Korean pine forest.Although carbohydrate metabolism pathways in the seed embryo are known to play crucial roles during seed dormancy and germination in P.koraiensis,it remains unclear whether these metabolic pathways function differentially across tissues.P.koraiensis seeds that had undergone different durations of moist chilling in their natural environment,yielding seeds with relatively deeper primary physiological dormancy(DDS)and seeds with released primary physiological dormancy(RDS).A non-targeted metabolomic analysis was conducted on the radicle and hypocotyl-cotyledon portions of both DDS and RDS,before and after a two-week incubation under favorable conditions.Under germination conditions,RDS and DDS showed divergent metabolic profiles,especially regarding carbohydrate metabolism.Specifically,RDS seeds showed significantly reduced substrates of respiratory metabolic pathways in both radicles and hypocotyl-cotyledons.Conversely,the intermediates of the carbohydrate metabolism pathway(particularly the tricarboxylic acid cycle)accumulating in radicles of DDS seeds under germination conditions.Moreover,in RDS,the carbohydrate metabolic pathways were more prevalent in the hypocotyl-cotyledon,while lysine degradation and ascorbate and aldarate metabolism were the dominant metabolic pathways in radicles.In contrast,the tricarboxylic acid cycle showed higher activity in DDS radicles compared to hypocotyl-cotyledons.We further demonstrated that carbohydratemetabolic pathways continue to play a dominant role in both dormancy maintenance and germination processes of P.koraiensis seeds.Notably,the carbohydratemetabolism in radicles likely exerts more critical regulatory functions in these two physiological processes compared to that in cotyledon and hypocotyl tissues.展开更多
Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis ind...Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis induction,and immune-modulatory activities of extracts from three herbal medicines with historical use in traditional medicine—Acanthopanax sessiliflorus,Phragmites communis,and Pinus densiflora,as well as their combined extract(GMAS 01/COM),on human lung cancer cells(A549)and normal cell lines,including murine macrophages(RAW 264.7)and human keratinocytes(HaCaT).Methods:Plant extracts were prepared using aqueous extraction,sonication,and rotary evaporation.The total phenolic and flavonoid contents were quantified using the Folin-Ciocalteu and AlCl3 colorimetric methods,respectively.Antioxidant potential was evaluated via 2,2-Diphenyl-1-picrylhydrazyl(DPPH)scavenging and reducing power assays.Cytotoxicity was assessed using an MTT assay,while reactive oxygen species(ROS)generation was quantified using a 2′,7′-Dichlorodihydrofluorescein diacetate(DCFH-DA)assay.Anticancer properties,including colony formation inhibition and migration suppression,were examined using colony formation and wound healing assays.The expression of apoptotic and inflammatory mediators was analysed through qPCR.Results:GMAS 01 selectively induced apoptosis in A549 cells without cytotoxic effects on RAW264.7 and HaCaT cells.Mechanistically,it elevated intracellular ROS and activated the intrinsic mitochondrial apoptotic pathway,evidenced by p53 upregulation,increased Bax,and decreased Bcl-2 expression.GMAS 01 also significantly inhibited colony formation and migration in A549 cells.In RAW264.7 cells,it reduced nitric oxide(NO)production and downregulated iNOS,COX-2,IL-6,and IL-8,indicating strong immunomodulatory activity.Conclusion:GMAS 01 exhibits potent antioxidant,anti-inflammatory,and anticancer effects,likely mediated through ROS-induced mitochondrial apoptosis.However,mechanistic interpretations are limited by the absence of protein-level validation and pathway inhibition studies.Upcoming studies should aim to verify the underlying mechanisms and evaluate their potential for real-world application.展开更多
To investigate the genetic variation patterns of multiple traits in Pinus sibirica half_sibling families introduced to the Greater Khingan Range,this study aims to establish a comprehensive trait evaluation system bas...To investigate the genetic variation patterns of multiple traits in Pinus sibirica half_sibling families introduced to the Greater Khingan Range,this study aims to establish a comprehensive trait evaluation system based on genetic parameters and identify fast-growing,high-quality genetic materials.The findings provide scientific support for advanced-generation seed orchard development and multiobjective genetic improvement.A total of 66 half-sibling families from a 26-year-old trial plantation of the Tomsk seed source were evaluated.Eleven traits were measured,including growth traits(tree height,diameter at breast height,volume,and crown width),morphological traits(lateral branch diameter),wood quality traits(Pilodyn value),and needle traits(fresh weight,dry weight,moisture content,needle length,and needle width).Genetic parameters were estimated using variance component decomposition.Superior families with favorable performance in both growth and wood density traits were identified using Best Linear Unbiased Prediction(BLUP)weighted by genetic correla-tion coefficients.Additionally,individual tree selection was conducted based on growth traits using the index selection method.Significant genetic differences among families(Z ratio>1.50)were observed for 10 traits,including growth,wood density,and needle traits.The phenotypic coefficient of variation(PCV:5.05%-62.50%)and genetic coefficient of variation(GCV:2.19%-13.81%)exhibited substantial heterogeneity.Volume displayed the highest variation(PCV=62.50%,GCV=13.81%),while the highest family heritability was observed for the needle length-to-width ratio(h2=78.30%),and the highest individual heritability was rec-orded for needle moisture content(H2=95.22%).Genetic correlations analysis revealed strong positive associ-ations between volume and tree height(r=0.880),diameter at br-east height(r=0.968),and Pilodyn value(r=0.508).Using the BLUP method,13 superior families(e.g.,Families 59,11 and 51)were identified,with an average genetic gain in volume of 13.377%and a family retention rate of 70%,significantly exceeding the population mean(65.10%).Th-rough index selection,94 elite individual trees were selected,52.14%of which originated from superior families.The genetic gain in individual tree volume reached 26.80%,with the within family gain for elite individuals increasing to 28.47%.This study establishes the first multitrait genetic evaluation model for P.sibirica and proposes a“familyindividual”collaborative selection strategy.The selected superior families achieved avolume genetic gain of 3.864%-32.307%and an overall geneticgain of 2.729%-20.069%,while elite individual trees exhibited a volume genetic gain of 16.328%-52.716%.These findings would provide critical technical support for multi-objective breeding and seed orchard development in cold-temperate coniferous species.展开更多
Pinus armandii Franch.,a key native conifer in China,faces severe Dendroctonus armandi infestation,threatening forest ecosystems.Reducing infestation probability and enhancing resistance are essential for transforming...Pinus armandii Franch.,a key native conifer in China,faces severe Dendroctonus armandi infestation,threatening forest ecosystems.Reducing infestation probability and enhancing resistance are essential for transforming Pinus armandii forests.This study investigates the correlation between stand structure,topographic factors,and the probability of Pinus armandii infestation by Dendroctonus armandi.Based on these correlations,it selects suitable mixedspecies combinations of native tree species with low infestation probabilities that are adapted to regional characteristics.A random survey was conducted in 58 plots(6,021 trees)in Shennongjia.Logistic regression and analysis of variance(ANOVA)revealed:(1)Infestation rate increased with elevation,peaking at 84.38% above 2,000 m;between 1,500-2,100 m,probability rose 4.3%per 100 m elevation gain;(2)Steeper slopes(>25°)reduced infestation(46.03%),with risk decreasing 1.9%per 1°slope increase(0°-40°);(3)Larger DBH(>30 cm)trees had higher infestation(82.93%),increasing 4.5%per 1 cm DBH;(4)Higher species mingling(four neighboring non-Pinus trees)lowered infestation to 63.39%,reducing risk by 54.3%per mingling unit;(5)Healthy Pinus armandii were frequently neighbored by Litsea pungens,Carpinus cordata,Phellodendron chinense,and Betula platyphylla.Prioritizing slopes>25°and elevations<2,000 m for afforestation,mixed with Litsea pungens,Carpinus cordata,or Betula platyphylla,can mitigate infestation.These findings provide actionable strategies to enhance Pinus armandii forest resilience against Dendroctonus armandi threats.展开更多
Essential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential.Essential oils were characterized using GC-MS and GC/FID.In vitro antimicrobial,phytotoxic,antioxidant,and ins...Essential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential.Essential oils were characterized using GC-MS and GC/FID.In vitro antimicrobial,phytotoxic,antioxidant,and insecticidal activities were carried out using the direct contact and the fumigant assays,respectively.The chemical profile of the essential oils of the resins of P.pinea and P.brutia included mainlyα-pinene(21.39%and 25.40%),β-pinene(9.68%and 9.69%),and caryophyllene(9.12%and 4.81%).The essential oils of P.pinea and P.brutia exerted notable antimicrobial activities on Micrococcus luteus and Bacillus subtilis,insecticidal activities on Ephestia kuehniella eggs,phytotoxic activities on Lactuca sativa,Lepidium sativum,and Portulaca oleracea,as well as antioxidant potential.Indications of the biological activities of the essential oils suggest their use in the formulation of ecofriendly and biocompatible pharmaceuticals.展开更多
The Qinling Mountains is not only the geographical boundary between North and South China,but also the boundary between subtropical and warm temperate zones.It plays an important role in the geo-ecological pattern of ...The Qinling Mountains is not only the geographical boundary between North and South China,but also the boundary between subtropical and warm temperate zones.It plays an important role in the geo-ecological pattern of China.However,there is controversy about the specific location of this geographical boundary in academic community due to the complexity,transition and heterogeneity of the transitional zone,as well as the differences in the delimitation indicators and research purposes.To further reveal the characteristics of the North-South transitional zone and clarify the specific location of the geo-ecological boundary between North and South China,combined with SRTM topographic data,temperature and precipitation data,Pinus massoniana forest and Pinus tabulaeformis forest,which represent subtropical coniferous forest in South China and temperate coniferous forest in North China respectively,were chosen to analyze their spatial distributions in the Qinling-Daba Mountains and the climatic conditions at their boundary with the climatic indexes of annual precipitation,the coldest month(January) average temperature,the warmest month(July) average temperature and the annual average temperature.The results show that:(1) Pinus massoniana and Pinus tabulaeformis forests and the climate indicators of their boundary can be used as one of the vegetation-climate indexes for the delimitation of subtropical and warm temperate zones.The boundary between the subtropical coniferous forest(Pinus massoniana forest) and temperate coniferous forest(Pinus tabulaeformis forest) is located along the south slope of Funiu Mountain to the north edge of Hanzhong Basin(the south slope of Qinling Mountains) at an altitude of 1000–1200 m,where the climatic indictors are stable:the annual precipitation is about 750–1000 mm,the annual average temperature is about 12–14℃,the coldest monthly average temperature is 0–4℃,and the warmest monthly average temperature is about 22–26℃.(2) It can be more scientifically to delimitate the boundary of subtropical and warm temperate zones in China by comprehensively considering the vegetation-climate indicators.Additionally,the boundary between subtropical and warm temperate zones in Qinling-Daba Mountains should be a transitional zone consisting of the boundaries of coniferous forests,broad-leaved forests and shrubs between subtropical and warm temperate zones.The results provide a scientific basis for the selection of delimitation index of subtropical and warm temperate zones.展开更多
Southeast Asia(SEA)has seen strong climatic oscillations and fluctuations in sea levels during the Quaternary.The impact of past climate changes on the evolution and distribution of local flora in SEA is still poorly ...Southeast Asia(SEA)has seen strong climatic oscillations and fluctuations in sea levels during the Quaternary.The impact of past climate changes on the evolution and distribution of local flora in SEA is still poorly understood.Here we aim to infer how the Quaternary climate change affects the evolutionary process and range shifts in two pine species.We investigated the population genetic structure and diversity using cytoplasmic DNA markers,and performed ecological niche modeling to reconstruct the species past distribution and to project range shift under future climates.We found substantial gene flow across the continuous distribution of the subtropical Pinus yunnanensis.In contrast,the tropical Pinus kesiya showed a strong population structure in accordance with its disjunct distribution across montane islands in Indochina and the Philippines.A broad hybrid zone of the two species occurs in southern Yunnan.Asymmetric introgression from the two species was detected in this zone with dominant mitochondrial gene flow from P.yunnanensis and chloroplast gene flow from P.kesiya.The observed population structure suggests a typical postglaciation expansion in P.yunnanensis,and a glacial expansion and interglacial contraction in P.kesiya.Ecological niche modeling supports the inferred demographic history and predicts a decrease in range size for P.kesiya under future climates.Our results suggest that tropical pine species in SEA have undergone evolutionary trajectories different from high latitude species related to their Quaternary climate histories.We also illustrate the need for urgent conservation actions in this fragmented landscape.展开更多
In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growt...In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growth of these seedlings.In particular,the effects of these fungi on the fungal community structure in the rhizosphere soil of the seedlings were investigated.Inoculation with Trichoderma harzianum E15 and Suillus luteus N94 significantly(P<0.05)promoted the growth of the Pinus sylvestris seedlings.The non-metric multidimensional scaling(NMDS)results indicated a significant difference(P<0.05)between the fungal community structures in the rhizosphere soil of the annual and biennial seedlings.In the rhizosphere soil of annual seedlings,the main fungi were Ascomycota,Basidiomycota,Zygomycota.Ascomycota,Basidiomycota,Mortierellomycota,and p-unclassified-k-Fungi were the main fungi in the rhizosphere soil of biennial seedlings.The dominant genus in the rhizosphere soil and a key factor promoting the growth of the annual and the biennial seedlings was Trichoderma,Suillus,respectively.Both of them were negatively correlated with the relative abundance of microbial flora in the symbiotic environment.Trichoderma had a significant promoting effect on the conversion of total phosphorus,total nitrogen,ammonium nitrogen,nitrate nitrogen,and the organic matter in the rhizosphere soil of the seedlings,while Suillus significantly promoted the conversion of organic matter and total phosphorus.展开更多
We employed capacitance to evaluate the kinship and interspecific variation of homoploid hybrid conifer Pinus densata,P.tabuliformis,P.yunnanensis and artificial hybrids of P.tabuliformis(maternal parent)and P.yunnane...We employed capacitance to evaluate the kinship and interspecific variation of homoploid hybrid conifer Pinus densata,P.tabuliformis,P.yunnanensis and artificial hybrids of P.tabuliformis(maternal parent)and P.yunnanensis(paternal parent)which were cultivated and selected in the common garden experiment.By measuring capacitance spectra under different voltage frequencies,we could differentiate different germplasms based on the electrical response.We aims to demonstrate that P.densata as the hybrid of P.tabuliformis and P.yunnanensis based on the capacitance values of the species,and to provide new evidence to the previously known biological evidence,as well as and the parental effect on the hybrids.Our results revealed that capacitance values between the species are significantly different in the spectra where P.yunnanensis positioned at the lowest and P.densata was much higher than all other species,indicating that P.densata had possessed a great capacity to store electrical energy.The capacitance spectra of P.densata and the artificial hybrid are not similar,which rejected our hypothesis.Both of the capacitance values of P.densata and the hybrids were closer to P.tabuliformis than to P.yunnanensis,which shows that the maternal influence was stronger than the paternal influence.Correlation analysis on the relationship between capacitance and fitnessrelated characteristics showed that capacitance is negatively correlated to mortality rate,and positively correlated with second-year survival rate.High capacitance values of P.densata and some of the hybrids reveal their superior adaptability to harsh environment in the Tibet Plateau.We concluded that capacitance as a new indicator for plant fitness and evolution evidence of homoploid hybrid conifers.展开更多
Understanding how tree species respond to drought in their natural environment is needed to predict forest adaptation and management practices under global environmental changes.This study was carried out to determine...Understanding how tree species respond to drought in their natural environment is needed to predict forest adaptation and management practices under global environmental changes.This study was carried out to determine and compare physiological and biochemical responses to variations in environmental conditions during summer drought of mixed natural stands of Pinus nigra Arn.subsp.pallasiana(Lamb.)Holmboe and Pinus brutia Ten.Midday xylem water potential(ψmd),water relations,photosynthetic pigments,total soluble sugar and proline contents were investigated during the growing season.ψmd followed a similar seasonal trend in both species but P.nigra subsp.pallasiana had higher ψmd than P.brutia.The ψmd gradually decreased from June,reached its lowest value in August,and then increased again.Gradual decreases in the osmotic potential at turgor loss point(ψnTLP)were observed during the summer.Generally,ψnTLP was lower in P.brutia.Total soluble sugars decreased from April to June for P.brutia,then increased and stayed relatively constant August to October.Similar changes were found at lower values in P.nigra subsp.pallasiana.Prolin accumulation and photosynthetic pigments were higher in P.brutia.The results indicate that physiological and biochemical responses of both species against changing environmental conditions were in different degrees but followed similar trends.P.nigra subsp.pallasiana is more sensitive to summer drought than P.brutia in their natural environment.展开更多
By investigation, studying and discussion for 9 years, the determination that it should be Pinus sibirica growing in the Mohe county, Daxing'anling Mountains area, China, rather thanpinus koraiensis there, is made...By investigation, studying and discussion for 9 years, the determination that it should be Pinus sibirica growing in the Mohe county, Daxing'anling Mountains area, China, rather thanpinus koraiensis there, is made to correct a false argument in some works from 1950s' till now that Pinus koraiensis area limit reach Daxing'anling Mountain. The results are as follows: (1) The species so called as Pinus koraiensis found in northwest of Daxing'anling Mountain is Pinus Sibirica indeed; There arc no natural distribution of Pinus Koraiensis in Daxing'anling Mountain. Its northwest area limit only reach Shcngshan, Aihui county; (3) There is about 500km of space between the area limits of Pinus koraiensis and that of Pinus sibirica, no mixed growing area. According to above, a sketch map is made to describe different area limit of the two species at Daxing'anling Mountain and nearby.展开更多
Pine wilt disease(PWD)is one of the most devastating diseases of Pinu s spp.and is caused by the pine wood nematode(PWN),Bursaphelenchus xylophilus(Steiner&Buhrer)Nickle.To study adaptation of PWN to survive in ho...Pine wilt disease(PWD)is one of the most devastating diseases of Pinu s spp.and is caused by the pine wood nematode(PWN),Bursaphelenchus xylophilus(Steiner&Buhrer)Nickle.To study adaptation of PWN to survive in hosts that differ in resistance,we examined the self-regulatory characteristics of PWN at the biological and molecular levels early in the interaction.Two-year-old susceptible Pinus thunbergii and resistant Pinus taeda were selected for this experiment,and changes in PWNs after inoculation were assessed.q RT-PCR was used to detect changes in genes related to PWN pathogenicity and detoxification.The results showed that the migration and reproductive abilities of PWNs in P.thunbergii were stronger than those of PWNs in P.taeda.After 7 d,the number of nematodes in P.thunbergii was approximately 3.2-fold higher than in P.taeda.After 15 d,the number of nematodes in P.thunbergii was approximately twofold higher than that in P.taeda.Because PWN can adjust its sex ratio after infection,we compared the sex ratio of uninoculated PWNs,to that in the two pine species.In P.thunbergii,the female to male ratio first decreased and then stabilized over time;in P.taeda first decreased and then increased.Relative fat accumulation in PWNs increased significantly after the PWNs entered the tree body;the accumulation rate in P.thunbergii was higher than in P.taeda at 7 d,but lower after 15 d.Scanning electron microscopy(SEM)showed significantly more bacteria on the surface of PWNs in P.taeda compared with PWNs in susceptible P.thunbergii.At 12 h after inoculation,the expression of genes related to cell-wall degradation(Bx Beta1-4 and Bxpel1),effectors(Bx CDP1,Bx Sap B1),and active oxygen metabolism(Bxy-ctl-1 and Bx GST3)was 2–6×higher in the resistant pine than in the susceptible one.In contrast,in PWNs,the expression of autophagy-related genes Bx ATG1 and Bx ATG16 was 1.5–2 times higher in P.thunbergii than in P.taeda.These results indicate that the interaction between PWNs and pine trees with different resistance levels elicits a series of physiological and molecular adaptations that affect nematode reproduction and virulence.This study will help elucidate the adaptive mechanisms of PWN in different pine trees.展开更多
The dwarf mountain pine(Pinus mugo)and the Pyrenean pine(P. uncinata)constitute a pair of closely related coniferous taxa of poorly resolved evolutionary history and affinity,which inhabit numerous stands scattered ov...The dwarf mountain pine(Pinus mugo)and the Pyrenean pine(P. uncinata)constitute a pair of closely related coniferous taxa of poorly resolved evolutionary history and affinity,which inhabit numerous stands scattered over subalpine environments of European mountain ranges.The aim of the study was to investigate their phylogeography and mutual relationships,shedding new light on their taxonomy and the past of the alpine flora.Previous evolutionary reconstructions of the mountain pines relied mainly on bi-parentally or paternally inherited markers that quickly homogenize between populations,showing rather shallow and recent differentiation of gene pools.Therefore,to contrast these pictures,we analyzed diversity and differentiation within a large set of new mitochondrial loci,inherited in maternal line and distributed by seeds at short geographical distances.Samples of the taxa were taken from 27 natural populations representing their rangewide distributions-17 populations of P.mugo and 10 of P.uncinata.All markers appeared polymorphic,providing a total of 31 multilocus haplotypes.Two of the loci proved to be species-diagnostic and nearly fixed between analyzed samples.Distribution of mitotypes indicate that allopatric populations of the taxa constitute separate mitochondrial haplogroups,and the two mountain pines have independent evolutionary history.However,introgression of P.mugo mitotypes by P.uncinata specimens revealed in the species contact zone in Western Alps shows that their speciation is not fully completed.展开更多
基金funded by Scion's Strategic Science Investment Fund(SSIF)the Forest Growers Levy Trust(FGLT)through the Resilient Forests Programme(Task No.A89220)。
摘要Pinus radiata(D.Don)dominates New Zealand's forestry industry,constituting 91%of plantations,and is among the world's most important plantation species.Given the socio-economic and environmental importance of this species,it is important to have accurate and precise projections over time to make efficient decisions for forest management and greenfield investments in afforestation projects,especially for permanent carbon forests.Future projections of any natural resource systems rely on modeling;however,the acceleration of climate change makes future projections of yield less certain.These challenges also impact national expectations of the contribution planted forests will provide to address climate change and meet international commitments under the Paris Agreement.Using a large national-scale set of contemporary ground-measured data(2013–2023),this study investigates the performance of two growth models developed over 30 years ago that are widely used by NZ plantation growers:1)the Pumice Plateau Model 1988(PPM88)and 2)the 300-index(including a model variant of regional drift).Model simulations were made using the FORECASTER modeling suite with geographic boundaries to adjust for drift in space and time.Basal area(BA,m2⋅ha-1)and volume(m3⋅ha-1)were simulated,and standard errors and goodness-of-fit metrics calculated up to a typical rotation age of 30 years.Model residuals were then separated and analysed for the main plantation growing regions.The models overpredicted observed growth by between 6.8%and 16.2%,but model predictions and errors varied significantly between regions.The results of this study provided clear evidence of divergence between the outputs of both models and the measured data.Finally,this study suggests future measures to address challenges posed by these discrepancies that will provide better information for forest management and investment decisions in a changing climate.
摘要Plant functional traits are key for understanding the adaptive strategies to environments,and fine roots play a crucial role in nutrient acquisition.Examining the variation of functional traits of fine root and soil physicochemical properties,and investigating their coupling relationships and dominating factors,could provide a theoretical foundation for ecological restoration in the burned forest.We established 12 plots within Pinus tabuliformis Carrière forests subjected to light,moderate and severe fire severities.Through detailed analysis of fine roots and soil physicochemical properties,we evaluated the variations and coupling effects in fine root functional traits and soil properties using the Coupling Coordination Degree Model and Partial Least Squares Path Modeling.Our results showed significant differences in the functional traits of fine root and soil physicochemical properties across fire severities(P<0.05).The coupling coordination degrees between fine root functional features and soil physicochemical properties ranged from 0.4 to 0.6,with the following order:unburned,moderate,severe and light severity.Forest fire negatively impacted the coupling coordination degree indirectly,primarily influenced by the direct positive effects of fine root morphological traits(e.g.,specific root length)and soil nutrient properties(e.g.,nitrogen and available phosphorus).The synergistic recovery of fine root-soil systems in Pinus tabuliformis forests was most pronounced following moderate fire severity,showing a medium-level coordination degree.For light-severity fires,enhancing fine root morphological characteristics through soil warming is recommended.In contrast,it is suggested to apply appropriate nitrogen and soil fertilizers for improving soil conditions after severe fire.
基金supported by the National Natural Science Foundation of China(32122059)Natural Science Foundation of Zhejiang Province(LQ21C030001,LQ22C160002)+2 种基金National Key Research and Development Program of China(2021YFD2200405,2021YFD2200402)the Youth Innovation Promotion Association of CAS(2023205)the Jiyang College Innovation Promotion Foundation(RQ2020B03).
摘要Global change drivers,including drought and nitrogen(N)deposition,exert a wide-ranging influence on tree growth and fitness.However,our current understanding of their combined effects is still limited.Non-structural carbohydrate(NSC)storage is an important physiological trait for tree acclimation to drought.It acts as an important mobile carbon reserve to support tree function when carbon fixation or transport are reduced under drought.It is crucial to investigate how tree species with different NSC storage characteristics(e.g.,storage level,partitioning)respond to drought events,and how N alters these patterns.We investigated the combined effects of drought(80%reduction in precipitation)and N addition(0,30,and 120 kg/ha/year)on the growth and NSC storage of Pinus koraiensis and Fraxinus mandshurica(dominant species in the forests of Northeast China)saplings over two consecutive growing seasons.The results indicated that P.koraiensis exhibited high tolerance to drought,with growth unaffected by drought alone until the mid-growing season in the second year.However,N addition reversed its drought acclimation by impairing root development and exacerbating carbon shortage.In contrast,F.mandshurica was sensitive to drought,it had significantly reduced growth at harvest despite a large amount of NSC accumulation.The present study highlights the contrasting effects of N deposition on drought adaptation in coexisting conifer and temperate broadleaf species,the conifer showing a higher risk of carbon deficiency with increasing N deposition(i.e.,a stronger reversal effect of N addition),whereas an earlier cessation of growth under drought defines a larger carbon safety margin for broadleaved species.These results have important implications for the development of adaptive forest management strategies such as to enhance the protection of conifers in the context of global change.
基金financially supported by the Biological BreedingNational Science and Technology Major Project(2023ZD0405806)the National Key R&D Program for the 14th Five-Year Plan in China(2022YFD2200304).
摘要The level of genetic variation within a breeding population affects the effectiveness of selection strategies for genetic improvement.The relationship between genetic variation level within Pinus tabuliformis breeding populations and selection strategies or selection effectiveness is not fully investigated.Here,we compared the selection effectiveness of combined and individual direct selection strategies using half-and full-sib families produced from advanced-generation P.tabuliformis seed orchard as our test populations.Our results revealed that,within half-sib families,average diameter at breast height(DBH),tree height,and volume growth of superior individuals selected by the direct selection strategy were higher by 7.72%,18.56%,and 31.01%,respectively,than those selected by the combined selection strategy.Furthermore,significant differences(P<0.01)were observed between the two strategies in terms of the expected genetic gains for average tree height and volume.In contrast,within full-sib families,the differences in tree average DBH,height,and volume between the two selection strategies were relatively minor with increase of 0.17%,2.73%,and 2.21%,respectively,and no significant differences were found in the average expected genetic gains for the studied traits.Half-sib families exhibited greater phenotypic and genetic variation,resulting in improved selection efficiency with the direct selection strategy but also introduced a level of inbreeding risk.Based on genetic distance estimates using molecular markers,our comparative seed orchard design analysis showed that the Improved Adaptive Genetic Programming Algorithm(IAPGA)reduced the average inbreeding coefficient by 14.36% and 14.73% compared to sequential and random designs,respectively.In conclusion,the combination of the direct selection strategy with IAPGA seed orchard design aimed at minimizing inbreeding offered an efficient approach for establishing advanced-generation P.tabuliformis seed orchards.
基金supported by the Science and Technology Development Program of Hebei Agricultural University,the Research on Molecular Mechanisms of Population Differentiation and Adaptation of Forest Pests and Insects under Environmental Stress(grant No.:30771739)Forest Pests and Diseases(grant No.:1528003)the National Natural Science Foundation of China for the study of community regulatory mechanisms of insect pest pandemics in larch plantation forests(Grant No.:32371882).
摘要The defense mechanisms induced in wild Chinese pine(Pinus tabuliformis)in response to herbivores are not well characterized,especially in the field.To address this knowledge gap,we established a biological model system to evaluate proteome variations in pine needles after feeding by the Chinese pine caterpillar(Dendrolimus tabulaeformis),a major natural enemy and dominant herbivore.Quantitative tandem mass tag(TMT)proteomics and bioinformatics were utilized to systematically identify differentially abundant proteins implicated in the induced defense response of Chinese pine.We validated key protein changes using parallel reaction monitoring(PRM)technology.Pathway analysis revealed that the induced defenses involved phenylpropanoid,coumarin,and flavonoid biosynthesis,among other processes.To elucidate the regulatory patterns underlying pine resistance,we determined the activities of defense enzymes and levels of physiological and biochemical compounds.In addition,the expression of upstream genes for key proteins was validated by qRT-PCR.Our results provide new molecular insights into the induced defense mechanisms in Chinese pine against this caterpillar in the field.A better understanding of these defense strategies will inform efforts to breed more-resistant pine varieties.
摘要Introduction Petrochemical feedstocks have been influential in pro-viding modern luxuries such as chemicals,energy,and materials.However,issues such as waste accumulation,environmental pollution,climate change,and resource depletion are common side-effects of this technology.With a growing population and its associated demand,there is an urgent need to switch to a cleaner means of production,whilst maintaining similar or better benefits than existing technology(Feng et al.2013;Neiva et al.2018,2020).
基金supported by the National Natural Science Foundation of China(31901300).
摘要Pinus koraiensis is the dominant and constructive species of the zonal vegetation in Northeast China,known as the mixed broadleaf-Korean pine forest.Although carbohydrate metabolism pathways in the seed embryo are known to play crucial roles during seed dormancy and germination in P.koraiensis,it remains unclear whether these metabolic pathways function differentially across tissues.P.koraiensis seeds that had undergone different durations of moist chilling in their natural environment,yielding seeds with relatively deeper primary physiological dormancy(DDS)and seeds with released primary physiological dormancy(RDS).A non-targeted metabolomic analysis was conducted on the radicle and hypocotyl-cotyledon portions of both DDS and RDS,before and after a two-week incubation under favorable conditions.Under germination conditions,RDS and DDS showed divergent metabolic profiles,especially regarding carbohydrate metabolism.Specifically,RDS seeds showed significantly reduced substrates of respiratory metabolic pathways in both radicles and hypocotyl-cotyledons.Conversely,the intermediates of the carbohydrate metabolism pathway(particularly the tricarboxylic acid cycle)accumulating in radicles of DDS seeds under germination conditions.Moreover,in RDS,the carbohydrate metabolic pathways were more prevalent in the hypocotyl-cotyledon,while lysine degradation and ascorbate and aldarate metabolism were the dominant metabolic pathways in radicles.In contrast,the tricarboxylic acid cycle showed higher activity in DDS radicles compared to hypocotyl-cotyledons.We further demonstrated that carbohydratemetabolic pathways continue to play a dominant role in both dormancy maintenance and germination processes of P.koraiensis seeds.Notably,the carbohydratemetabolism in radicles likely exerts more critical regulatory functions in these two physiological processes compared to that in cotyledon and hypocotyl tissues.
摘要Objectives:The phytochemical investigation of traditional herbal medicines holds significant promise for modern drug discovery,particularly in cancer therapy.This study aimed to evaluate the cytotoxicity,apoptosis induction,and immune-modulatory activities of extracts from three herbal medicines with historical use in traditional medicine—Acanthopanax sessiliflorus,Phragmites communis,and Pinus densiflora,as well as their combined extract(GMAS 01/COM),on human lung cancer cells(A549)and normal cell lines,including murine macrophages(RAW 264.7)and human keratinocytes(HaCaT).Methods:Plant extracts were prepared using aqueous extraction,sonication,and rotary evaporation.The total phenolic and flavonoid contents were quantified using the Folin-Ciocalteu and AlCl3 colorimetric methods,respectively.Antioxidant potential was evaluated via 2,2-Diphenyl-1-picrylhydrazyl(DPPH)scavenging and reducing power assays.Cytotoxicity was assessed using an MTT assay,while reactive oxygen species(ROS)generation was quantified using a 2′,7′-Dichlorodihydrofluorescein diacetate(DCFH-DA)assay.Anticancer properties,including colony formation inhibition and migration suppression,were examined using colony formation and wound healing assays.The expression of apoptotic and inflammatory mediators was analysed through qPCR.Results:GMAS 01 selectively induced apoptosis in A549 cells without cytotoxic effects on RAW264.7 and HaCaT cells.Mechanistically,it elevated intracellular ROS and activated the intrinsic mitochondrial apoptotic pathway,evidenced by p53 upregulation,increased Bax,and decreased Bcl-2 expression.GMAS 01 also significantly inhibited colony formation and migration in A549 cells.In RAW264.7 cells,it reduced nitric oxide(NO)production and downregulated iNOS,COX-2,IL-6,and IL-8,indicating strong immunomodulatory activity.Conclusion:GMAS 01 exhibits potent antioxidant,anti-inflammatory,and anticancer effects,likely mediated through ROS-induced mitochondrial apoptosis.However,mechanistic interpretations are limited by the absence of protein-level validation and pathway inhibition studies.Upcoming studies should aim to verify the underlying mechanisms and evaluate their potential for real-world application.
基金funded by the Key Science and Technology Program of the State Forestry Administration(Project No.:2001-31(1))the Scientific Research Project of Greater Khingan Range Forestry Group Co.,Ltd.(Project No.:s2023001)。
摘要To investigate the genetic variation patterns of multiple traits in Pinus sibirica half_sibling families introduced to the Greater Khingan Range,this study aims to establish a comprehensive trait evaluation system based on genetic parameters and identify fast-growing,high-quality genetic materials.The findings provide scientific support for advanced-generation seed orchard development and multiobjective genetic improvement.A total of 66 half-sibling families from a 26-year-old trial plantation of the Tomsk seed source were evaluated.Eleven traits were measured,including growth traits(tree height,diameter at breast height,volume,and crown width),morphological traits(lateral branch diameter),wood quality traits(Pilodyn value),and needle traits(fresh weight,dry weight,moisture content,needle length,and needle width).Genetic parameters were estimated using variance component decomposition.Superior families with favorable performance in both growth and wood density traits were identified using Best Linear Unbiased Prediction(BLUP)weighted by genetic correla-tion coefficients.Additionally,individual tree selection was conducted based on growth traits using the index selection method.Significant genetic differences among families(Z ratio>1.50)were observed for 10 traits,including growth,wood density,and needle traits.The phenotypic coefficient of variation(PCV:5.05%-62.50%)and genetic coefficient of variation(GCV:2.19%-13.81%)exhibited substantial heterogeneity.Volume displayed the highest variation(PCV=62.50%,GCV=13.81%),while the highest family heritability was observed for the needle length-to-width ratio(h2=78.30%),and the highest individual heritability was rec-orded for needle moisture content(H2=95.22%).Genetic correlations analysis revealed strong positive associ-ations between volume and tree height(r=0.880),diameter at br-east height(r=0.968),and Pilodyn value(r=0.508).Using the BLUP method,13 superior families(e.g.,Families 59,11 and 51)were identified,with an average genetic gain in volume of 13.377%and a family retention rate of 70%,significantly exceeding the population mean(65.10%).Th-rough index selection,94 elite individual trees were selected,52.14%of which originated from superior families.The genetic gain in individual tree volume reached 26.80%,with the within family gain for elite individuals increasing to 28.47%.This study establishes the first multitrait genetic evaluation model for P.sibirica and proposes a“familyindividual”collaborative selection strategy.The selected superior families achieved avolume genetic gain of 3.864%-32.307%and an overall geneticgain of 2.729%-20.069%,while elite individual trees exhibited a volume genetic gain of 16.328%-52.716%.These findings would provide critical technical support for multi-objective breeding and seed orchard development in cold-temperate coniferous species.
基金funded by the National Natural Science Foundation of China(Grant No.32371873)the National Natural Science Foundation of China(Grant No.32071683).
摘要Pinus armandii Franch.,a key native conifer in China,faces severe Dendroctonus armandi infestation,threatening forest ecosystems.Reducing infestation probability and enhancing resistance are essential for transforming Pinus armandii forests.This study investigates the correlation between stand structure,topographic factors,and the probability of Pinus armandii infestation by Dendroctonus armandi.Based on these correlations,it selects suitable mixedspecies combinations of native tree species with low infestation probabilities that are adapted to regional characteristics.A random survey was conducted in 58 plots(6,021 trees)in Shennongjia.Logistic regression and analysis of variance(ANOVA)revealed:(1)Infestation rate increased with elevation,peaking at 84.38% above 2,000 m;between 1,500-2,100 m,probability rose 4.3%per 100 m elevation gain;(2)Steeper slopes(>25°)reduced infestation(46.03%),with risk decreasing 1.9%per 1°slope increase(0°-40°);(3)Larger DBH(>30 cm)trees had higher infestation(82.93%),increasing 4.5%per 1 cm DBH;(4)Higher species mingling(four neighboring non-Pinus trees)lowered infestation to 63.39%,reducing risk by 54.3%per mingling unit;(5)Healthy Pinus armandii were frequently neighbored by Litsea pungens,Carpinus cordata,Phellodendron chinense,and Betula platyphylla.Prioritizing slopes>25°and elevations<2,000 m for afforestation,mixed with Litsea pungens,Carpinus cordata,or Betula platyphylla,can mitigate infestation.These findings provide actionable strategies to enhance Pinus armandii forest resilience against Dendroctonus armandi threats.
基金supported by the Scientific Research Fund(OKU-BAP)of Osmaniye Korkut Ata University,Osmaniye,Turkey
摘要Essential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential.Essential oils were characterized using GC-MS and GC/FID.In vitro antimicrobial,phytotoxic,antioxidant,and insecticidal activities were carried out using the direct contact and the fumigant assays,respectively.The chemical profile of the essential oils of the resins of P.pinea and P.brutia included mainlyα-pinene(21.39%and 25.40%),β-pinene(9.68%and 9.69%),and caryophyllene(9.12%and 4.81%).The essential oils of P.pinea and P.brutia exerted notable antimicrobial activities on Micrococcus luteus and Bacillus subtilis,insecticidal activities on Ephestia kuehniella eggs,phytotoxic activities on Lactuca sativa,Lepidium sativum,and Portulaca oleracea,as well as antioxidant potential.Indications of the biological activities of the essential oils suggest their use in the formulation of ecofriendly and biocompatible pharmaceuticals.
基金National Natural Science Foundation of China,No.41871350Scientific and Technological Basic Resources Survey Project,No.2017FY100900。
摘要The Qinling Mountains is not only the geographical boundary between North and South China,but also the boundary between subtropical and warm temperate zones.It plays an important role in the geo-ecological pattern of China.However,there is controversy about the specific location of this geographical boundary in academic community due to the complexity,transition and heterogeneity of the transitional zone,as well as the differences in the delimitation indicators and research purposes.To further reveal the characteristics of the North-South transitional zone and clarify the specific location of the geo-ecological boundary between North and South China,combined with SRTM topographic data,temperature and precipitation data,Pinus massoniana forest and Pinus tabulaeformis forest,which represent subtropical coniferous forest in South China and temperate coniferous forest in North China respectively,were chosen to analyze their spatial distributions in the Qinling-Daba Mountains and the climatic conditions at their boundary with the climatic indexes of annual precipitation,the coldest month(January) average temperature,the warmest month(July) average temperature and the annual average temperature.The results show that:(1) Pinus massoniana and Pinus tabulaeformis forests and the climate indicators of their boundary can be used as one of the vegetation-climate indexes for the delimitation of subtropical and warm temperate zones.The boundary between the subtropical coniferous forest(Pinus massoniana forest) and temperate coniferous forest(Pinus tabulaeformis forest) is located along the south slope of Funiu Mountain to the north edge of Hanzhong Basin(the south slope of Qinling Mountains) at an altitude of 1000–1200 m,where the climatic indictors are stable:the annual precipitation is about 750–1000 mm,the annual average temperature is about 12–14℃,the coldest monthly average temperature is 0–4℃,and the warmest monthly average temperature is about 22–26℃.(2) It can be more scientifically to delimitate the boundary of subtropical and warm temperate zones in China by comprehensively considering the vegetation-climate indicators.Additionally,the boundary between subtropical and warm temperate zones in Qinling-Daba Mountains should be a transitional zone consisting of the boundaries of coniferous forests,broad-leaved forests and shrubs between subtropical and warm temperate zones.The results provide a scientific basis for the selection of delimitation index of subtropical and warm temperate zones.
基金supported by the Strategic Priority Research Program of CAS(no.XDA20070301 to Z.-K.Z.)the National Natural Science Foundation of China(NSFC 31770701,NSFC 31870425)+1 种基金a joint NSFC-Yunnan Government Grant(U1502264)supported by a grant from the CASTWAS President's Fellowship Program.
摘要Southeast Asia(SEA)has seen strong climatic oscillations and fluctuations in sea levels during the Quaternary.The impact of past climate changes on the evolution and distribution of local flora in SEA is still poorly understood.Here we aim to infer how the Quaternary climate change affects the evolutionary process and range shifts in two pine species.We investigated the population genetic structure and diversity using cytoplasmic DNA markers,and performed ecological niche modeling to reconstruct the species past distribution and to project range shift under future climates.We found substantial gene flow across the continuous distribution of the subtropical Pinus yunnanensis.In contrast,the tropical Pinus kesiya showed a strong population structure in accordance with its disjunct distribution across montane islands in Indochina and the Philippines.A broad hybrid zone of the two species occurs in southern Yunnan.Asymmetric introgression from the two species was detected in this zone with dominant mitochondrial gene flow from P.yunnanensis and chloroplast gene flow from P.kesiya.The observed population structure suggests a typical postglaciation expansion in P.yunnanensis,and a glacial expansion and interglacial contraction in P.kesiya.Ecological niche modeling supports the inferred demographic history and predicts a decrease in range size for P.kesiya under future climates.Our results suggest that tropical pine species in SEA have undergone evolutionary trajectories different from high latitude species related to their Quaternary climate histories.We also illustrate the need for urgent conservation actions in this fragmented landscape.
摘要In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growth of these seedlings.In particular,the effects of these fungi on the fungal community structure in the rhizosphere soil of the seedlings were investigated.Inoculation with Trichoderma harzianum E15 and Suillus luteus N94 significantly(P<0.05)promoted the growth of the Pinus sylvestris seedlings.The non-metric multidimensional scaling(NMDS)results indicated a significant difference(P<0.05)between the fungal community structures in the rhizosphere soil of the annual and biennial seedlings.In the rhizosphere soil of annual seedlings,the main fungi were Ascomycota,Basidiomycota,Zygomycota.Ascomycota,Basidiomycota,Mortierellomycota,and p-unclassified-k-Fungi were the main fungi in the rhizosphere soil of biennial seedlings.The dominant genus in the rhizosphere soil and a key factor promoting the growth of the annual and the biennial seedlings was Trichoderma,Suillus,respectively.Both of them were negatively correlated with the relative abundance of microbial flora in the symbiotic environment.Trichoderma had a significant promoting effect on the conversion of total phosphorus,total nitrogen,ammonium nitrogen,nitrate nitrogen,and the organic matter in the rhizosphere soil of the seedlings,while Suillus significantly promoted the conversion of organic matter and total phosphorus.
摘要We employed capacitance to evaluate the kinship and interspecific variation of homoploid hybrid conifer Pinus densata,P.tabuliformis,P.yunnanensis and artificial hybrids of P.tabuliformis(maternal parent)and P.yunnanensis(paternal parent)which were cultivated and selected in the common garden experiment.By measuring capacitance spectra under different voltage frequencies,we could differentiate different germplasms based on the electrical response.We aims to demonstrate that P.densata as the hybrid of P.tabuliformis and P.yunnanensis based on the capacitance values of the species,and to provide new evidence to the previously known biological evidence,as well as and the parental effect on the hybrids.Our results revealed that capacitance values between the species are significantly different in the spectra where P.yunnanensis positioned at the lowest and P.densata was much higher than all other species,indicating that P.densata had possessed a great capacity to store electrical energy.The capacitance spectra of P.densata and the artificial hybrid are not similar,which rejected our hypothesis.Both of the capacitance values of P.densata and the hybrids were closer to P.tabuliformis than to P.yunnanensis,which shows that the maternal influence was stronger than the paternal influence.Correlation analysis on the relationship between capacitance and fitnessrelated characteristics showed that capacitance is negatively correlated to mortality rate,and positively correlated with second-year survival rate.High capacitance values of P.densata and some of the hybrids reveal their superior adaptability to harsh environment in the Tibet Plateau.We concluded that capacitance as a new indicator for plant fitness and evolution evidence of homoploid hybrid conifers.
基金The work was supported by the Suleyman Demirel University,Scientific Research Projects Coordination Unit(Project Number:4513-YL1-15).
摘要Understanding how tree species respond to drought in their natural environment is needed to predict forest adaptation and management practices under global environmental changes.This study was carried out to determine and compare physiological and biochemical responses to variations in environmental conditions during summer drought of mixed natural stands of Pinus nigra Arn.subsp.pallasiana(Lamb.)Holmboe and Pinus brutia Ten.Midday xylem water potential(ψmd),water relations,photosynthetic pigments,total soluble sugar and proline contents were investigated during the growing season.ψmd followed a similar seasonal trend in both species but P.nigra subsp.pallasiana had higher ψmd than P.brutia.The ψmd gradually decreased from June,reached its lowest value in August,and then increased again.Gradual decreases in the osmotic potential at turgor loss point(ψnTLP)were observed during the summer.Generally,ψnTLP was lower in P.brutia.Total soluble sugars decreased from April to June for P.brutia,then increased and stayed relatively constant August to October.Similar changes were found at lower values in P.nigra subsp.pallasiana.Prolin accumulation and photosynthetic pigments were higher in P.brutia.The results indicate that physiological and biochemical responses of both species against changing environmental conditions were in different degrees but followed similar trends.P.nigra subsp.pallasiana is more sensitive to summer drought than P.brutia in their natural environment.
摘要By investigation, studying and discussion for 9 years, the determination that it should be Pinus sibirica growing in the Mohe county, Daxing'anling Mountains area, China, rather thanpinus koraiensis there, is made to correct a false argument in some works from 1950s' till now that Pinus koraiensis area limit reach Daxing'anling Mountain. The results are as follows: (1) The species so called as Pinus koraiensis found in northwest of Daxing'anling Mountain is Pinus Sibirica indeed; There arc no natural distribution of Pinus Koraiensis in Daxing'anling Mountain. Its northwest area limit only reach Shcngshan, Aihui county; (3) There is about 500km of space between the area limits of Pinus koraiensis and that of Pinus sibirica, no mixed growing area. According to above, a sketch map is made to describe different area limit of the two species at Daxing'anling Mountain and nearby.
基金supported by the major emergency project in science and technology of National Forestry and Grassland Administration(ZD202001)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
摘要Pine wilt disease(PWD)is one of the most devastating diseases of Pinu s spp.and is caused by the pine wood nematode(PWN),Bursaphelenchus xylophilus(Steiner&Buhrer)Nickle.To study adaptation of PWN to survive in hosts that differ in resistance,we examined the self-regulatory characteristics of PWN at the biological and molecular levels early in the interaction.Two-year-old susceptible Pinus thunbergii and resistant Pinus taeda were selected for this experiment,and changes in PWNs after inoculation were assessed.q RT-PCR was used to detect changes in genes related to PWN pathogenicity and detoxification.The results showed that the migration and reproductive abilities of PWNs in P.thunbergii were stronger than those of PWNs in P.taeda.After 7 d,the number of nematodes in P.thunbergii was approximately 3.2-fold higher than in P.taeda.After 15 d,the number of nematodes in P.thunbergii was approximately twofold higher than that in P.taeda.Because PWN can adjust its sex ratio after infection,we compared the sex ratio of uninoculated PWNs,to that in the two pine species.In P.thunbergii,the female to male ratio first decreased and then stabilized over time;in P.taeda first decreased and then increased.Relative fat accumulation in PWNs increased significantly after the PWNs entered the tree body;the accumulation rate in P.thunbergii was higher than in P.taeda at 7 d,but lower after 15 d.Scanning electron microscopy(SEM)showed significantly more bacteria on the surface of PWNs in P.taeda compared with PWNs in susceptible P.thunbergii.At 12 h after inoculation,the expression of genes related to cell-wall degradation(Bx Beta1-4 and Bxpel1),effectors(Bx CDP1,Bx Sap B1),and active oxygen metabolism(Bxy-ctl-1 and Bx GST3)was 2–6×higher in the resistant pine than in the susceptible one.In contrast,in PWNs,the expression of autophagy-related genes Bx ATG1 and Bx ATG16 was 1.5–2 times higher in P.thunbergii than in P.taeda.These results indicate that the interaction between PWNs and pine trees with different resistance levels elicits a series of physiological and molecular adaptations that affect nematode reproduction and virulence.This study will help elucidate the adaptive mechanisms of PWN in different pine trees.
基金The authors acknowledge with thanks the financial support from the Polish National Science Centre(Grant No.UMO-2015/19/B/NZ9/00024)The authors also thank Krystyna Boratynska from the Institute of Dendrology,Polish Academy of Sciences,for providing plant material.
摘要The dwarf mountain pine(Pinus mugo)and the Pyrenean pine(P. uncinata)constitute a pair of closely related coniferous taxa of poorly resolved evolutionary history and affinity,which inhabit numerous stands scattered over subalpine environments of European mountain ranges.The aim of the study was to investigate their phylogeography and mutual relationships,shedding new light on their taxonomy and the past of the alpine flora.Previous evolutionary reconstructions of the mountain pines relied mainly on bi-parentally or paternally inherited markers that quickly homogenize between populations,showing rather shallow and recent differentiation of gene pools.Therefore,to contrast these pictures,we analyzed diversity and differentiation within a large set of new mitochondrial loci,inherited in maternal line and distributed by seeds at short geographical distances.Samples of the taxa were taken from 27 natural populations representing their rangewide distributions-17 populations of P.mugo and 10 of P.uncinata.All markers appeared polymorphic,providing a total of 31 multilocus haplotypes.Two of the loci proved to be species-diagnostic and nearly fixed between analyzed samples.Distribution of mitotypes indicate that allopatric populations of the taxa constitute separate mitochondrial haplogroups,and the two mountain pines have independent evolutionary history.However,introgression of P.mugo mitotypes by P.uncinata specimens revealed in the species contact zone in Western Alps shows that their speciation is not fully completed.