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Valosin-containing protein,neural proteopathies,and implications for neural regeneration 认领 引用
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作者 Jae-Geun Lee Eun-Ji Lee +1 位作者 Hoon Ryu Jeong-Soo Lee 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第7期2920-2921,共2页
Proteostasis,also known as protein homeostasis,is a tightly regulated cellular quality control process that ensures the balance of protein synthesis,folding,posttranslational modifications,and degradation.Maintaining ... Proteostasis,also known as protein homeostasis,is a tightly regulated cellular quality control process that ensures the balance of protein synthesis,folding,posttranslational modifications,and degradation.Maintaining proteostasis is vital for cellular function,organismal health,and longevity.The disru ption of proteostasis can lead to a range of detrimental effects,including accelerated aging,compromised cellular function,and even cell death,manifesting in numerous human diseases(Hipp et al.,2019). 展开更多
关键词 valosin containing protein protein homeostasis protein homeostasisis neural proteopathies quality control process posttranslational modifications proteostasis protein synthesis
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Proteomic Analysis Revealing Protein Expression in Rice Grain Containing Differential Protein and Amino Acid Contents 认领 引用
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作者 Krishnendu CHATTOPADHYAY Arabinda MAHANTY +6 位作者 Nutan MOHARANA Shuvendu Shekhar MOHAPATRA Torit Baran BAGCHI Koushik CHAKRABORTY Joydeep BANERJEE Bimal Prasanna MOHANTY Trilochan MOHAPATRA 《Rice science》 SCIE CAS CSCD 2026年第1期9-14,I0012-I0027,共6页
CR Dhan 310(CRD310),a biofortified rice variety,contains a significantly higher level of grain protein compared with its recurrent parent Naveen(NV),as well as most adapted high-yielding rice varieties in India.Althou... CR Dhan 310(CRD310),a biofortified rice variety,contains a significantly higher level of grain protein compared with its recurrent parent Naveen(NV),as well as most adapted high-yielding rice varieties in India.Although a limited investigation depicted that CRD310 contained higher levels of glutelin and some essential amino acids,detailed biochemical,molecular,and cellular mechanisms remain to be studied.As one of the means to identify the proteins and understand the underlying mechanism of higher proteins accumulation in grains of CRD310,the comparative proteomics was undertaken on grains of CRD310 and NV at the yellow ripening stage. 展开更多
关键词 grain protein rice grain comparative proteomics essential amino acids identify proteins protein accumulation biochemical mechanisms amino acidsdetailed
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Opportunities and challenges in protein structure prediction 认领 引用
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作者 Wenkai Wang Baoquan Su +1 位作者 Chenxiao Xiang Jianyi Yang 《Quantitative Biology》 CAS CSCD 2026年第2期17-24,共8页
Deep learning methods,particularly exemplified by AlphaFold2,have revolutionized the field of protein structure prediction-an achievement recognized by the 2024 Nobel Prize in Chemistry awarded to its core developers.... Deep learning methods,particularly exemplified by AlphaFold2,have revolutionized the field of protein structure prediction-an achievement recognized by the 2024 Nobel Prize in Chemistry awarded to its core developers.Despite this remarkable achievement,the broader protein folding problem is far from solved.Key challenges—each representing opportunities for future breakthroughs—include single-sequence structure prediction,modeling protein dynamics,accurately predicting multimeric complexes,and effectively incorporating experimental constraints.Here we review recent progress in these key frontiers and share our perspective on future directions. 展开更多
关键词 cryo-EM deep learning protein complexes protein dynamics protein structure prediction single-sequence prediction
Dementia risk prediction in individuals with prediabetes or diabetes:A novel multi-protein score with biological pathway analysis 认领 引用
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作者 Yuanyuan Zhang Yu Huang +8 位作者 Junyu Xue Yiwei Zhang Sisi Yang Yanjun Zhang Ziliang Ye Xiaoqin Gan Yiting Wu Fanfan Hou Xianhui Qin 《General Psychiatry》 CAS CSCD 2026年第1期55-64,共10页
Background Individuals with prediabetes or diabetes face elevated dementia risk,yet robust prediction tools and mechanistic insights remain limited.Aims This study aimed to develop and validate a proteinbased risk sco... Background Individuals with prediabetes or diabetes face elevated dementia risk,yet robust prediction tools and mechanistic insights remain limited.Aims This study aimed to develop and validate a proteinbased risk score for dementia prediction in this high-risk population while elucidating underlying biological pathways and therapeutic targets.Methods Utilising data from 10 433 UK Biobank participants with prediabetes or diabetes and proteomic profiling(2911 plasma proteins measured),we developed a dementia protein risk score in a training set(n=6514)and validated it in testing(n=2790) and external cohorts(n=1129).Results In the training set,23 out of 2911 proteins were selected.In the testing set,compared with the basic model(age and sex,C-index:0.78;95% confidence interval [CI]0.74-0.82),the dementia protein risk score(C-index:0.84;95% CI 0.81-0.88) significantly improved the performance in predicting incident dementia(C-index increase:0.06;95% CI 0.02-0.12),while cardiovascular risk factors,ageing and dementia incidence risk factors(C-index:0.80;95% Cl0.76-0.83) and apolipoprotein E(APOE;age and sex included,C-index:0.81;95% CI 0.77-0.85) had no significant improvement.Six key proteins(glial fibrillary acidic protein [GFAP],neurofilament light polypeptide[NEFL],Brevican core protein [BCAN],protein MENT[MENT],APOE and growth/differentiation factor 15[GDF15]) captured the most predictive power.Pathway analyses implicated extracellular matrix remodelling and cholesterol metabolism,whereas Mendelian randomisation identified causal roles for APOE,haematopoietic prostaglandin D synthase(HPGDS),BAG family molecular chaperone regulator 3(BAG3) and GDF15.Nine proteins were prioritised as druggable targets,including HPGDS,with existing Food and Drug Administration-approved drugs.Conclusions This study establishes a highly accurate protein-based risk score for dementia prediction(including 6-23 proteins) in individuals with prediabetes or diabetes,uncovering actionable biological pathways and therapeutic targets.The findings enable precision risk stratification and accelerate translational opportunities for dementia prevention in this population. 展开更多
关键词 prediabetes plasma proteins proteomic profiling multi protein score dementia p mechanistic insights develop validate proteinbased risk score dementia risk prediction
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Bromodomain-containing protein 4 knockdown promotes neuronal ferroptosis in a mouse model of subarachnoid hemorrhage 认领 引用 被引量：3
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作者 Peng Lu Fan Zhang +8 位作者 Lei Yang Yijing He Xi Kong Kecheng Guo Yuke Xie Huangfan Xie Bingqing Xie Yong Jiang Jianhua Peng 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第2期715-729,共15页
Neuronal cell death is a common outcome of multiple pathophysiological processes and a key factor in neurological dysfunction after subarachnoid hemorrhage.Neuronal ferroptosis in particular plays an important role in... Neuronal cell death is a common outcome of multiple pathophysiological processes and a key factor in neurological dysfunction after subarachnoid hemorrhage.Neuronal ferroptosis in particular plays an important role in early brain injury.Bromodomain-containing protein 4,a member of the bromo and extraterminal domain family of proteins,participated in multiple cell death pathways,but the mechanisms by which it regulates ferroptosis remain unclear.The primary aim of this study was to investigate how bromodomain-containing protein 4 affects neuronal ferroptosis following subarachnoid hemorrhage in vivo and in vitro.Our findings revealed that endogenous bromodomain-containing protein 4 co-localized with neurons,and its expression was decreased 48 hours after subarachnoid hemorrhage of the cerebral cortex in vivo.In addition,ferroptosis-related pathways were activated in vivo and in vitro after subarachnoid hemorrhage.Targeted inhibition of bromodomain-containing protein 4 in neurons increased lipid peroxidation and intracellular ferrous iron accumulation via ferritinophagy and ultimately led to neuronal ferroptosis.Using cleavage under targets and tagmentation analysis,we found that bromodomain-containing protein 4 enrichment in the Raf-1 promoter region decreased following oxyhemoglobin stimulation in vitro.Furthermore,treating bromodomain-containing protein 4-knockdown HT-22 cell lines with GW5074,a Raf-1 inhibitor,exacerbated neuronal ferroptosis by suppressing the Raf-1/ERK1/2 signaling pathway.Moreover,targeted inhibition of neuronal bromodomain-containing protein 4 exacerbated early and long-term neurological function deficits after subarachnoid hemorrhage.Our findings suggest that bromodomain-containing protein 4 may have neuroprotective effects after subarachnoid hemorrhage,and that inhibiting ferroptosis could help treat subarachnoid hemorrhage. 展开更多
关键词 bromodomain-containing protein 4 cell death early brain injury ferritinophagy ferroptosis neurological deficits neuron oxidative stress RAF proto-oncogene serinehreonine-protein kinase(Raf-1) subarachnoid hemorrhage
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Serum protein induced by vitamin K absence or antagonist-Ⅱpredicts aggressive tumor biology in alpha-fetoprotein-normal hepatocellular carcinoma 认领 引用 被引量：1
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作者 Zaigham Abbas Darayus P Gazder +2 位作者 Muhammad Ali Qadeer Minaam Abbas Zeeshan Hyder 《World Journal of Gastrointestinal Oncology》 SCIE 2026年第2期89-96,共8页
BACKGROUND Patients with hepatocellular carcinoma(HCC)beyond the Milan criteria or with portal vein tumor thrombosis are often excluded from the transplant list owing to aggressive biology and recurrence risk.While hi... BACKGROUND Patients with hepatocellular carcinoma(HCC)beyond the Milan criteria or with portal vein tumor thrombosis are often excluded from the transplant list owing to aggressive biology and recurrence risk.While high alpha-fetoprotein(AFP)signals aggressiveness,the behavior of normal AFP HCC with elevated protein induced by vitamin K absence/antagonist-Ⅱ(PIVKA-Ⅱ)is less defined.AIM To assess the prognostic value of PIVKA-Ⅱin normal AFP HCC.METHODS Retrospective cohort of 113 patients with normal AFP and normal or elevated PIVKA-Ⅱ.“Aggressive”tumors were defined as beyond Milan and/or portal vein tumor thrombosis(n=63);others were non-aggressive(n=50).Receiver operating characteristic curve analysis identified PIVKA-Ⅱcut-offs.RESULTS This study included 78 men and 35 women;mean age 58.4±11.1 years;62.8%with decompensated cirrhosis.PIVKA-Ⅱwas higher in aggressive tumors:Median 2785 mAU/mL(interquartile range:222-8152)vs 239 mAU/mL(interquartile range:55-727),P400 mAU/mL was strongly associated with aggressive tumor phenotype(adjusted odds ratio=5.16,P=0.001).All the 29 patients with≥4000 mAU/mL were in the aggressive group(PPV:1.0).All thresholds were dataset-derived.CONCLUSION In normal AFP HCC,PIVKA-Ⅱdiscriminates aggressive biology.A cut-off of 1609.5 mAU/mL balances sensitivity and specificity;400 mAU/mL favors sensitivity;≥4000 mAU/mL delineates an ultra-high-risk subgroup.Findings support the incorporation of PIVKA-Ⅱinto risk stratification. 展开更多
关键词 Alpha fetoprotein Protein induced by vitamin K absence/antagonist-Ⅱ Des-gamma carboxy prothrombin Hepatocellular carcinoma Biomarkers Aggressive tumor
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Proteolytic shedding of the prion protein:Uncovering“new”biological implications of a conserved cleavage event 认领 引用
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作者 Feizhi Song Valerija Kovac +4 位作者 Behnam Mohammadi Josephine E.Pippi Vladka Curin Serbec Markus Glatzel Hermann C.Altmeppen 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第6期2329-2330,共2页
Novel insights into complex biological processes very often critically depend on the establishment of new potent read-out tools and improved protocols.A lot has been learned over the past four decades on physiological... Novel insights into complex biological processes very often critically depend on the establishment of new potent read-out tools and improved protocols.A lot has been learned over the past four decades on physiological functions and,importantly,disease-related roles of the prion protein(PrP),a relatively broadly expressed membrane-anchored glycoprotein with high levels in several cell types of the nervous and immune system and with well-established key roles in different progressive and fatal neurodegenerative protein misfolding diseases(proteopathies). 展开更多
关键词 prion protein prp protein misfolding diseases prion protein neurodegenerative diseases read tools insights complex biological processes disease related roles physiological functions
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A missing link in the METTL3 chain:Chaperone regulation of METTL3 as a BAG:HSP70 client protein 认领 引用
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作者 Joseph M.Irvin Gail V.W.Johnson 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第9期4273-4274,共2页
Following translation,newly synthesized proteins must navigate a turbulent free energy landscape that threatens to trap them in misfolded or nonfunctional conformation.To reach their native states,many proteins are as... Following translation,newly synthesized proteins must navigate a turbulent free energy landscape that threatens to trap them in misfolded or nonfunctional conformation.To reach their native states,many proteins are assisted by molecular chaperones that transiently facilitate folding and assembly without being part of the final structure.One such molecular scaffold is the heat shock protein 70(HSP70) chaperone family,which acts in concert with Bcl-2-associated athanogene(BAG) co-chaperones to regulate a wide array of clients(Figure 1). 展开更多
关键词 mettl molecular scaffold bag co chaperones client protein heat shock protein molecular chaperones protein folding
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TaPRR95 regulates wheat flowering time and yield partially by interacting with the CONSTANS-like protein 认领 引用
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作者 Xinxin Cheng Lijun Zhang +9 位作者 Ying Xu Yongchao Hao Peng Jiang Shams ur Rehman Shisheng Chen Lingrang Kong Shuxin Zhang Hongwei Wang Yan Zhao Guiping Wang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第7期3086-3089,共4页
Highlights·A TaPRR95-A haplotype reduces flowering time and increases grain yield in wheat.·Protein-protein interaction between TaPRR95-A and TaCOL16-D modulates the TaFT1-mediated flowering pathway.·A ... Highlights·A TaPRR95-A haplotype reduces flowering time and increases grain yield in wheat.·Protein-protein interaction between TaPRR95-A and TaCOL16-D modulates the TaFT1-mediated flowering pathway.·A validated functional marker enables rapid identification of the target haplotype and provides a practical tool for markerassisted selection in wheat breeding.As a staple cereal crop cultivated worldwide,common wheat(Triticum aestivum L.)accounts for approximately 35%of global dietary energy requirements(Sharma and Sharma 2025).However,agricultural yields are currently under significant pressure due to the combined impacts of anthropogenic climate change and geopolitical conflicts(Ortiz-Bobea et al.2021;Hultgren et al.2025),which threatens global food security.These challenges necessitate the urgent development of molecular breeding approaches to improve wheat production sustainably. 展开更多
关键词 taprr validated functional marker identification target haplotype flowering time constans protein yield markerassisted selection protein protein interaction
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Investigating the interactive adhesion ability between Limosilactobacillus reuteri DSM17938 and HT-29 cells regulated by soybean proteins and soybean peptides 认领 引用
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作者 Yinxiao Zhang Chi Zhang +3 位作者 Jingyi Wang He Li Xinqi Liu Xianyong Liu 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第5期2198-2206,共9页
In this study,digested soybean protein(dspr)and peptides(dspe)were found to have different effects on the adhesion ability of Limosilactobacillus reuteri DSM17938 and HT-29 cells.Results found that dspr and dspe could... In this study,digested soybean protein(dspr)and peptides(dspe)were found to have different effects on the adhesion ability of Limosilactobacillus reuteri DSM17938 and HT-29 cells.Results found that dspr and dspe could increase L.reuteri adhesion ability by affecting their auto-aggregation and hydrophobicity during their stationary phase.The moonlighting protein may play a dominant role affected by dspe in the adhesion process of L.reuteri from stationary phase.Moreover,dspr and dspe also enhanced the adhesion ability of HT-29cells to L.reuteri DSM17938,and they might play different role at different stages of adhesion.dspr mainly promoted the expression of adhesion-related genes before L.reuteri adhesion.dspe increased them after the adhesion of L.reuteri from log phase and dspr mainly enhanced their expression after stationary phase L.reuteri adhesion.Soybean protein and peptides may therefore be considered as a potential effective modulator of L.reuteri adhesion to intestinal tract. 展开更多
关键词 Soybean protein Soybean peptides Limosilactobacillus reuteri DSM17938 Adhesion Surface protein
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Ferulic acid alleviates the reduction of beef myofibrillar protein digestibility mediated by protein oxidation:insight from peptide release 认领 引用
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作者 Yantao Yin Yongcun Lu +2 位作者 Naiyong Xiao Shucheng Liua Wangang Zhang 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第3期1179-1188,共10页
This study investigated the protective effect of ferulic acid(FA) on the digestibility of oxidized beef myofibrillar protein(MP).MP were treated with varying concentrations of FA(20,40,and 80 μmol/g pro) and then exp... This study investigated the protective effect of ferulic acid(FA) on the digestibility of oxidized beef myofibrillar protein(MP).MP were treated with varying concentrations of FA(20,40,and 80 μmol/g pro) and then exposed to oxidation.The results showed that FA inhibited carbonyl in a dose-dependent manner.Fluorescence spectroscopy and molecular docking studies revealed FA was bonded to MP via hydrophobic interactions and hydrogen bonds.Sodium dodecyl sulfate polyacrylamide gel electrophoresis and transmission electron microscope analysis indicated that FA mitigated the aggregation of oxidized MP.Peptidomic analysis showed FA protected 34.24% peptides recover from oxidation loss,and theses recovered peptide were mainly distributed in hydrophobic regions and lysine sites.In vitro digestion showed FA remarkably improved digestibility of oxidized MP(P < 0.05),with 80 μmol/g pro FA mitigating 67.85% loss in digestibility.Overall,FA effectively preserved the release of peptides by inhibiting hydrophobic aggregation and oxidation of lysine residues,thereby alleviating the decrease in digestibility of oxidized MP. 展开更多
关键词 Meat Protein oxidation Protein digestibility Ferulic acid Peptidomic
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Tropomyosin 3 regulates tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma to promote malignant progression in gastric cancer 认领 引用
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作者 Zhen-Qi Yue Yi-Wu Yuan +5 位作者 He-Song Xu Xue-Qin Li Jin-Heng Gan Yong-Hui Zou Luo-Jun Fan Lin Xin 《World Journal of Gastroenterology》 SCIE CAS 2026年第22期88-110,共23页
BACKGROUND Tropomyosin 3(TPM3)has been implicated in the progression of several cancers;however,its specific role and underlying molecular mechanisms in gastric cancer(GC)remain unclear.The current research aimed to i... BACKGROUND Tropomyosin 3(TPM3)has been implicated in the progression of several cancers;however,its specific role and underlying molecular mechanisms in gastric cancer(GC)remain unclear.The current research aimed to investigate the role of TPM3 in the onset and advancement of GC,along with the related molecular pathways.AIM To investigate TPM3’s role in enhancing GC malignancy and elucidates the underlying molecular mechanisms.METHODS TPM3 expression in GC tissues was evaluated using bioinformatics analysis.Protein expression levels were determined using western blotting,and mRNA levels were measured through quantitative real-time PCR.Cellular functional assays,including cell counting kit-8 assay,5-ethynyl-2-deoxyuridine incorporation,colony formation,wound-healing,and transwell migration/invasion,were conducted to assess the effects of TPM3 and tyrosine 3-monooxygenaseryptophan 5-monooxygenase activation protein gamma(YWHAG)on GC cell proliferation,migration,invasion,and metastatic potential.Cell cycle progression and apoptosis were analyzed using flow cytometry.A subcutaneous xenograft model in nude mice was employed to assess the tumorigenic potential of GC cells,while a tail vein injection model was used to evaluate their invasive and metastatic potential in vivo.RESULTS TPM3 was significantly upregulated in GC tissues and cells,and its high expression correlated with poor patient prognosis.Silencing TPM3 markedly inhibited GC cell proliferation,invasion,and metastasis while promoting apoptosis.TPM3 interacted with YWHAG,and TPM3 knockdown reduced YWHAG expression.Notably,the inhibitory effects of TPM3 silencing on GC progression were partially reversed by overexpressing YWHAG,confirming YWHAG as a key downstream effector.Furthermore,TPM3 knockdown suppressed activation of the mitogen-activated protein kinase pathway in a YWHAG-dependent manner.In vivo experiments demonstrated that increased TPM3 expression significantly promoted GC tumor growth and metastasis,whereas silencing YWHAG effectively attenuated this effect.CONCLUSION TPM3 promotes GC progression by regulating YWHAG and activating the mitogen-activated protein kinase signaling pathway.These findings identify the TPM3/YWHAG axis as a potential therapeutic target for GC intervention. 展开更多
关键词 Tropomyosin 3 Tyrosine 3-monooxygenaseryptophan 5-monooxygenase activation protein gamma Mitogenactivated protein kinase signaling pathway Gastric cancer Molecular mechanism
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Role of prion protein in mediating the synaptotoxic effects of tau oligomers:Implications for Alzheimer’s disease and related tauopathies 认领 引用
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作者 Roberto Chiesa Luana Fioriti +1 位作者 Gianluigi Forloni Claudia Balducci 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第8期3527-3528,共2页
Alzheimer’s disease(AD)and other tauopathies are characterized by the accumulation of misfolded tau protein,which forms toxic oligomers that contribute to synaptic dysfunction and neuronal loss.Here,we briefly discus... Alzheimer’s disease(AD)and other tauopathies are characterized by the accumulation of misfolded tau protein,which forms toxic oligomers that contribute to synaptic dysfunction and neuronal loss.Here,we briefly discuss recent findings indicating that the cellular prion protein(PrPC)plays a critical role in mediating the synaptotoxic effects of tau oligomers(TauOs),offering new insights into disease pathogenesis and potential therapeutic strategies. 展开更多
关键词 tau oligomers tauos offering cellular prion protein prpc plays tau oligomers neuronal loss synaptic dysfunction disease pathogenesis toxic oligomers misfolded tau proteinwhich
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Succinylation:A novel regulatory axis in cholelithiasis-insights from lysine acetyltransferase 2A/adenosine monophosphate-activated protein kinase signaling 认领 引用
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作者 He-Rui Wei Fan Xiao 《World Journal of Gastroenterology》 SCIE CAS 2026年第9期171-173,共3页
Cholelithiasis represents a common clinical condition within the digestive tract and continues to pose a substantial global health challenge,largely due to its high rate of recurrence and a scarcity of effective non-s... Cholelithiasis represents a common clinical condition within the digestive tract and continues to pose a substantial global health challenge,largely due to its high rate of recurrence and a scarcity of effective non-surgical interventions.Although protein succinylation has been widely characterized as a post-translational modification,its implications in cholelithiasis pathogenesis had remained poorly defined.A groundbreaking study by Wang et al demonstrates that lysine acetyltransferase 2A-mediated succinylation of adenosine monophosphate-activated protein kinase suppresses cholelithiasis.This work not only provides novel mechanistic insights into cholelithiasis but also establishes succinylation as a promising therapeutic target,thereby addressing a critical knowledge gap in the field. 展开更多
关键词 Cholelithiasis Succinylation Lysine acetyltransferase 2A Adenosine monophosphate-activated protein kinase Protein post-translational modification
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Protein post-translational modification in plants:regulation and beyond 认领 引用 被引量：1
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作者 Zhenxiang Li Liqian Qin +7 位作者 Dongjiao Wang Tianzhen Cui Yanlan Liang Shoujian Zang Tingting Sun Wanying Zhao Qibin Wu Youxiong Que 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第5期969-984,共16页
Post-translational modification(PTM)of proteins is a crucial regulatory mechanism in plant cells,enabling rapid and purposeful regulation of their functions.Modified proteins play various roles in signaling pathways,i... Post-translational modification(PTM)of proteins is a crucial regulatory mechanism in plant cells,enabling rapid and purposeful regulation of their functions.Modified proteins play various roles in signaling pathways,including plant growth and development,plant metabolism,and the response to adversity stress.In recent years,there has been an increase in the number of studies focusing on plant PTM maps and functional analysis.Here we aim to review the PTM types in plants,especially in horticultural plants and tropical crops,the interactions between and among PTMs,and more importantly,the underlying pathways and functions.Additionally,the potential application of PTMs in breeding is briefly discussed.This paper focuses on plant PTMs and provides a foundation for further investigation into the functions and regulatory mechanisms of PTMs in plant proteins as well as for crop improvement. 展开更多
关键词 Protein post-translational modification PTM types Pathways and functions Crosstalk Regulatory mechanism Crop improvement
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Potential mechanism of the effect of heat stress on milk protein synthesis revealed by integrated metabolomic and proteomic analyses 认领 引用 被引量：1
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作者 Jia Zeng Diming Wang +3 位作者 Huizeng Sun Hongyun Liu Feng‑Qi Zhao Jianxin Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第2期809-821,共13页
Background This study was conducted to investigate the impact of varying degrees of heat stress on milk protein synthesis in dairy cows using comprehensive analyses of metabolomics and proteomics.Eighteen dairy cows w... Background This study was conducted to investigate the impact of varying degrees of heat stress on milk protein synthesis in dairy cows using comprehensive analyses of metabolomics and proteomics.Eighteen dairy cows were subjected to no heat stress(No-HS),mild heat stress(Mild-HS),and moderate heat stress(Mod-HS).Blood and milk samples were collected to determine the content and composition of amino acids(AA),and milk samples were used for metabolomic and proteomic analyses.Results Milk protein yield was significantly lower under Mild-HS and Mod-HS than No-HS(P<0.001).During Mild-HS,no significant difference was found in total AA concentration in both arterial(P=0.545)and venous blood(P=0.057),but arterial AA supply to the mammary gland significantly increased(P=0.045)when compared with No-HS.Under Mod-HS,the supply(P<0.001)and uptake(P=0.001)of total AA in the mammary gland decreased significantly,affecting the availability of precursors necessary for milk protein synthesis.Milk metabolomic analysis revealed that Mod-HS significantly impacted nucleotide metabolism,energy metabolism,and protein synthesis processes including translation,folding,and transport.Milk proteomic analysis showed significant downregulation of ribosomal and heat shock proteins which are crucial for protein synthesis and folding.Conclusions These findings suggest that heat stress disrupts AA utilization and energy metabolism in the mammary gland,leading to the reduced efficiency in milk protein synthesis and lowered milk protein yield.This study offers valuable insights into the metabolic and proteomic changes in dairy cows under heat stress,highlighting potential strategies to mitigate the adverse effects of heat stress on dairy production and milk quality. 展开更多
关键词 Dairy cow Heat stress Milk protein synthesis Multi??omics
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Low-density lipoprotein receptor–related protein 1 mediatesα-synuclein transmission from the striatum to the substantia nigra in animal models of Parkinson's disease 认领 引用 被引量：1
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作者 Hanjiang Luo Caixia Peng +5 位作者 Chengli Wu Chengwei Liu Qinghua Li Shun Yu Jia Liu Min Chen 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第4期1595-1606,共12页
α-Synuclein accumulation and transmission are vital to the pathogenesis of Parkinson's disease,although the mechanisms underlying misfoldedα-synuclein accumulation and propagation have not been conclusively dete... α-Synuclein accumulation and transmission are vital to the pathogenesis of Parkinson's disease,although the mechanisms underlying misfoldedα-synuclein accumulation and propagation have not been conclusively determined.The expression of low-density lipoprotein receptor–related protein 1,which is abundantly expressed in neurons and considered to be a multifunctional endocytic receptor,is elevated in the neurons of patients with Parkinson's disease.However,whether there is a direct link between low-density lipoprotein receptor–related protein 1 andα-synuclein aggregation and propagation in Parkinson's disease remains unclear.Here,we established animal models of Parkinson's disease by inoculating monkeys and mice withα-synuclein pre-formed fibrils and observed elevated low-density lipoprotein receptor–related protein 1 levels in the striatum and substantia nigra,accompanied by dopaminergic neuron loss and increasedα-synuclein levels.However,low-density lipoprotein receptor–related protein 1 knockdown efficiently rescued dopaminergic neurodegeneration and inhibited the increase inα-synuclein levels in the nigrostriatal system.In HEK293A cells overexpressingα-synuclein fragments,low-density lipoprotein receptor–related protein 1 levels were upregulated only when the N-terminus ofα-synuclein was present,whereas anα-synuclein fragment lacking the N-terminus did not lead to low-density lipoprotein receptor–related protein 1 upregulation.Furthermore,the N-terminus ofα-synuclein was found to be rich in lysine residues,and blocking lysine residues in PC12 cells treated withα-synuclein pre-formed fibrils effectively reduced the elevated low-density lipoprotein receptor–related protein 1 andα-synuclein levels.These findings indicate that low-density lipoprotein receptor–related protein 1 regulates pathological transmission ofα-synuclein from the striatum to the substantia nigra in the nigrostriatal system via lysine residues in theα-synuclein N-terminus. 展开更多
关键词 α-synuclein dopaminergic neurodegeneration internalization low-density lipoprotein receptor-related protein 1 lysine pre-formed fibril movement disorder nigrostriatal system Parkinson's disease transmission
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Processing effects on the characteristics of oat protein solutions: The role of enzymatic treatment and pH-shifting with mild heating 认领 引用
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作者 Anthony Suryamiharja Xiping Gong +2 位作者 Minghe Wang Kevin Mis Solval Hualu Zhou 《Grain & Oil Science and Technology》 EI CAS 2026年第1期1-10,共10页
Oat milk has gained widespread consumer acceptance for its creamy texture,β-glucan content,and environmental sustainability.However,its relatively low protein content(typically 2-3 g/serving)presents a nutritional li... Oat milk has gained widespread consumer acceptance for its creamy texture,β-glucan content,and environmental sustainability.However,its relatively low protein content(typically 2-3 g/serving)presents a nutritional limitation,largely due to poor protein solubility.This study investigated how processing conditions influence protein content and functional stability in oat-based systems by applying two treatments:(1)α-amylase enzymatic hydrolysis,and(2)pH-shifting(from pH 7 to 12 and back)with mild heating(50℃for 10 or 30 min).Oat protein solutions were formulated from two sources:oat flour(OF)and oat protein isolate(OPI).Results suggests that α-amylase pretreatment effectively reduced starch-driven viscosity in OF,facilitating better sample handling and centrifugation.Following pH-shifting and heat treatment,both OF and OPI solutions showed significantly improved protein solubility,with protein content increased from 2.0 to~6.5 g/serving.These changes were accompanied by reduced precipitation,smaller particle sizes,and more negative zeta potential values,indicating enhanced colloidal stability.SDS-PAGE analysis revealed the presence of low-molecular-weight protein fractions,supporting increased solubilization.Fluorescence microscopy confirmed the formation of smaller,more uniformly dispersed particles in treated samples compared to controls.However,noticeable darkening or browning occurred under high-pH heating,indicating potential challenges in color control.The findings provide useful information for future industrial applications and product innovation in the plant-based beverage sector. 展开更多
关键词 Oat protein Protein enrichment Enzymatical treatment pH-shifting Heating
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ZNF219 inhibits porcine epidemic diarrhea virus by degrading viral S2 protein 认领 引用
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作者 Wenli Zhao Ning Kong +13 位作者 Daoliang Lan Yu Zhang Hailong Liu Chunyun Liu Wenzhen Qin Xinyu Yang Tian Liu Wu Tong Hai Yu Hao Zheng Guangzhi Tong Shengsong Xie Guangxu Xing Tongling Shan 《Virologica Sinica》 SCIE CAS CSCD 2026年第2期303-313,共11页
Since its discovery,porcine epidemic diarrhea virus(PEDV)has significantly affected the agricultural economy worldwide.The available commercialized coronavirus vaccines cannot adequately control emerging strains.There... Since its discovery,porcine epidemic diarrhea virus(PEDV)has significantly affected the agricultural economy worldwide.The available commercialized coronavirus vaccines cannot adequately control emerging strains.Therefore,investigating the correlation between viruses and antiviral host factors is necessary.In this study,we showed that zinc finger protein 219(ZNF219)was upregulated by viral nonstructural protein 12(nsp12)upon PEDV challenge.Moreover,ZNF219 inhibited the replication of PEDV through selective autophagic degradation of the PEDV S2 protein.ZNF219 recruited TRAF6,the ubiquitin E3 ligase,to ubiquitinate the PEDV S2 protein.After recognition,the ubiquitinated PEDV S2 protein was delivered to autolysosomes via the cargo receptor p62 for degradation by autophagy,thus inhibiting the proliferation of PEDV.To summarize,after sensing PEDV infection by recognizing the viral nsp12 protein,host cells upregulated the intracellular expression of ZNF219,which degraded the viral S2 protein by activating autophagy,thus suppressing viral replication.Our study revealed a novel antiviral mechanism involving ZNF219 and provided a novel target for preventing and treating PEDV. 展开更多
关键词 Zinc finger protein 219(ZNF219) Porcine epidemic diarrhea virus (PEDV) TRAF6 P62 PEDV S2 protein Selective autophagy
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Sustained release of branched-chain amino acids from slow-digesting whey enhances endurance running performance and improves skeletal muscle protein synthesis in mice 认领 引用
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作者 Juncai Leng Tingyi Zhou +5 位作者 Yiming Jiang Beibei Wang Shuoming Liu Shiqi Zhang Li Li Wei Zhao 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第1期191-201,共11页
Post-exercise whey protein isolate(WPI)supplement is beneficial for skeletal muscle recovery due to the stimulation of branched chain amino acids(BCAAs).This implies us that intake slow digestion rate of protein to su... Post-exercise whey protein isolate(WPI)supplement is beneficial for skeletal muscle recovery due to the stimulation of branched chain amino acids(BCAAs).This implies us that intake slow digestion rate of protein to sustain BCAAs releasing rate may facilitate muscle protein synthesis.To examine this hypothesis,we conducted a series of protein supplements including modified slow-digesting whey(SDW),whey,hydrolyzed whey and casein,orally to mice undergoing endurance running.Our results showed that the SDW gavage constant supplied BCAAs in the serum of mice within 6 h and significantly enhanced(P<0.01)endurance exercise capacity,compared to other groups.In addition,the SDW supplementation increased the crosssectional area of mice gastrocnemius fibers,as well as their muscle and liver glycogen content.It also increased the testosterone/cortisol ratio in serum and interleukin-6(IL-6)levels in muscle,while it decreased the tumor necrosis factor-alpha(TNF-α)levels and oxidative stress in muscle.Moreover,it may activate mechanistic target of rapamycin signaling by upregulating mRNA(bcat-1 and pgc-1α)expression.Thus,our findings illustrate that prolonged BCAAs supply duration promotes mice endurance running capacity and skeletal muscle growth,contributing to the advancement of sports nutrition practices. 展开更多
关键词 Whey protein isolate(WPI) Branched chain amino acids(BCAAs) Endurance running Muscle protein synthesis
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