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Building Information Modeling-Based Secondary Development System for 3D Modeling of Underground Pipelines 认领 引用 被引量：6
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作者 Jun Chen Rao Hu +1 位作者 Xianfeng Guo Feng Wu 《Computer Modeling in Engineering & Sciences》 SCIE EI 2020年第5期647-660,共14页
Underground pipeline networks constitute a major component of urban infrastructure,and thus,it is imperative to have an efficient mechanism to manage them.This study introduces a secondary development system to effici... Underground pipeline networks constitute a major component of urban infrastructure,and thus,it is imperative to have an efficient mechanism to manage them.This study introduces a secondary development system to efficiently model underground pipeline networks,using the building information modeling(BIM)-based software Revit.The system comprises separate pipe point and tubulation models.Using a Revit application programming interface(API),the spatial position and attribute data of the pipe points are extracted from a pipeline database,and the corresponding tubulation data are extracted from a tubulation database.Using the Family class in Revit API,the cluster in the self-built library of pipe point is inserted into the spatial location and the attribute data is added;in the same way,all pipeline instances in the pipeline system are created.The extension and localization of the model accelerated the modeling speed.The system was then used in a real construction project.The expansion of the model database and rapid modeling made the application of BIM technology in three-dimensional visualization of underground pipeline networks more convenient.Furthermore,it has applications in pipeline engineering construction and management. 展开更多
关键词 Building information modeling secondary development underground pipeline 3D modeling visualization.
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VviAGL11 self-regulates and targets hormone-and secondary metabolism-related genes during seed development 认领 引用 被引量：5
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作者 Alessandra Amato Maria Francesca Cardone +7 位作者 Nallatt Ocarez Fiammetta Alagna Benedetto Ruperti Chiara Fattorini Riccardo Velasco Nilo Mejía Sara Zenoni Carlo Bergamini 《Horticulture Research》 SCIE CSCD 2022年第1期2866-2880,共15页
VviAGL11,the Arabidopsis SEEDSTICK homolog,has been proposed to have a causative role in grapevine stenospermocarpy.An association between a mutation in the coding sequence(CDS)and the seedless phenotype was reported,... VviAGL11,the Arabidopsis SEEDSTICK homolog,has been proposed to have a causative role in grapevine stenospermocarpy.An association between a mutation in the coding sequence(CDS)and the seedless phenotype was reported,however,no working mechanisms have been demonstrated yet.We performed a deep investigation of the full VviAGL11 gene sequence in a collection of grapevine varieties belonging to several seedlessness classes that revealed three different promoter-CDS combinations.By investigating the expression of the three VviAGL11 alleles,and by evaluating their ability to activate the promoter region,we observed that VviAGL11 self-activates in a specific promoter-CDS combination manner.Furthermore,by transcriptomic analyses on ovule and developing seeds in seeded and seedless varieties and co-expression approaches,candidate VviAGL11 targets were identified and further validated through luciferase assay and in situ hybridization.We demonstrated that VviAGL11 Wild Type CDS activates Methyl jasmonate esterase and Indole-3-acetate beta-glucosyltransferase,both involved in hormone signaling and Isoflavone reductase,involved in secondary metabolism.The dominant-negative effect of the mutated CDS was also functionally ectopically validated in target induction.VviAGL11 was shown to co-localize with its targets in the outer seed coat integument,supporting its direct involvement in seed development,possibly by orchestrating the crosstalk among MeJA,auxin,and isoflavonoids synthesis.In conclusion,the VviAGL11 expression level depends on the promoter-CDS allelic combination,and this will likely affect its ability to activate important triggers of the seed coat development.The dominant-negative effect of the mutated VviAGL11 CDS on the target genes activation was molecularly validated.A new regulatory mechanism correlating VviAGL11 haplotype assortment and seedlessness class in grapevine is proposed. 展开更多
关键词 investigating expression arabidopsis seedstick seedless phenotype vviagl gene sequence grapevine stenospermocarpyan hormone signaling secondary metabolism seed development
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Virtual Reality and Visual Simulation Technology in Platform Construction with Four Bucket Foundations Based on Secondary Development of Vega 认领 引用 被引量：2
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作者 张浦阳 丁红岩 +1 位作者 白智华 朱奇 《Transactions of Tianjin University》 EI CAS 2013年第6期398-403,共6页
The platform with bucket foundations can penetrate and migrate by underpressure/positive pressure technique caused by pumping water out/in the bucket. However, the construction process of bucket foundations cannot be ... The platform with bucket foundations can penetrate and migrate by underpressure/positive pressure technique caused by pumping water out/in the bucket. However, the construction process of bucket foundations cannot be clearly observed and effectively controlled due to the special nature of sea environment. By using an advanced simulation development tool of Multigen Creator, the visual construction simulation program for the platform with bucket foundations was developed to set up the virtual reality system with interaction control and observation in every view angle based on the secondary development technology of Vega platform. The application results show that the method is feasible and effective by simulating the whole construction process for the platform with four bucket foundations. 展开更多
关键词 bucket foundation virtual reality secondary development simulation Vega
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Effects of Plant Secondary Metabolite Nicotine on Growth and Development of Frankliniella occidentalis 认领 引用 被引量：1
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作者 Liu Jiani Chen Zebin +2 位作者 Yu Lei Xu Shengguang Zhang Yuyu 《Plant Diseases and Pests》 2018年第2期21-24,36,共4页
[Objective] The paper was to explore the chemical and molecular mechanisms of Frankliniella occidentalis, an important quarantine pest, against toxic substances. [Method] F. occidentalis were evaluated after continuou... [Objective] The paper was to explore the chemical and molecular mechanisms of Frankliniella occidentalis, an important quarantine pest, against toxic substances. [Method] F. occidentalis were evaluated after continuous exposure to artificial diets containing nicotine from the 2^（nd） and the 3^（rd）instars for five generations, to determine the larval weight, pupal weight, and larval development duration. [Result] The larval development delayed and nicotine treatment significantly inhibited the weight gain of larvae. Compared to the control, the inhibition rates of larval weight and pupal weight were declined from 45% to 20% and from 25% to 4% respectively after feeding the 2^（nd）instars with nicotine for five generations.Nicotine delayed the larval growth and prolonged the generation duration of F. occidentalis. Nicotine showed more significant inhibitory effect on the lower instar larvae. With the increasing generations of secondary culture, the inhibitory effect of nicotine on larval weight and pupal weight was weakened. The developmental period of larvae was shortened, and the generation duration of F. occidentalis was shortened. [Conclusion] Nicotine affects the growth and development of F. occidentalis. F. occidentalis will gradually increase the adaptability to nicotine toxic substances after selfregulation for a few generations, and relieve the inhibitory effect of toxic substances. 展开更多
关键词 Plant secondary metabolite Nicotine Frankliniella occidentolis Growth and development
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Optimization techniques for the secondary development of old gas fields in the Sichuan Basin and their application 认领 引用
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作者 Xia Chongshuang Liu Linqing +1 位作者 Zhang Li Peng Xian 《Natural Gas Industry B》 2016年第6期595-606,共12页
After nearly 60 years of development,many old gasfields in the Sichuan Basin have come to middleelate development stages with low pressure and low yield,and some are even on the verge of abandonment,but there are plen... After nearly 60 years of development,many old gasfields in the Sichuan Basin have come to middleelate development stages with low pressure and low yield,and some are even on the verge of abandonment,but there are plenty remaining gas resources still undeveloped.Analysis shows that gasfields which have the conditions for the secondary development are faced with many difficulties.For example,it is difficult to produce low permeable reserves and to unset the hydraulic seal which is formed by active formation water.In this paper,therefore,the technical route and selection conditions of old gasfields for the secondary development were comprehensively elaborated with its definition as the beginning.Firstly,geological model forward modeling and production performance inversion characteristic curve diagnosis are performed by using the pressure normalization curve and the identification and quantitative description method for multiple sets of storageeseepage body of complex karst fractureecavity systems is put forward,after the multiple storageeseepage body mode of fractureecavity systems is established.Combined with the new occurrence mode of gas and water in U-shape pipes,a new calculation technology for natural gas reserves of multiple fractureecavity systems with strong water invasion is developed.Secondly,a numerical model of poreecavityefracture triple media is built,and simulation and result evaluation technology for the production pattern of“drainage by horizontal wells+gas production by vertical wells”in bottom-water fracture and cavity gas reservoirs with strong water invasion is developed.Thirdly,the geological model of gas reservoirs is reconstructed with the support of the integration technologies which are formed based onfine gas reservoir description.Low permeable reserves of gas reservoirs are evaluated based on each classification.The effective producing ratio is increased further by using the technologies of well pattern optimization,horizontal-well geosteering and staged acid fracturing.And fourthly,overall simulation,optimization and prediction technology for regional pipeline net-works is developed by building a multi-node multi-link gas transmission pipeline network model.Application shows that this technology plays an important role in productivity construction,recovery factor improvement,production decline delay and production stabilization of old gasfields. 展开更多
关键词 Sichuan Basin Old gas fiekd Secondary development Active water invasion Multiple sets of storage-seepage body Drainage system Well pattern Pipe network Low permeable reserves producing Recovery factor
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The novel gene BrYL1,encoding an inosine monophosphate dehydrogenase,is involved in chloroplast development in Chinese cabbage 认领 引用
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作者 Shuangjuan Yang Wenhua Zhao +9 位作者 Zhaojun Wang Xiaochun Wei Yanyan Zhao Zhiyong Wang Henan Su Wenjing Zhang Haohui Yang Lin Li Yuxiang Yuan Xiaowei Zhang 《Horticultural Plant Journal》 SCIE CAS CSCD 2025年第6期2281-2284,共4页
Purines are building blocks for DNA and RNA,found as the energy currency of cells(ATP and GTP),used as signaling molecules(cGMP,cAMP and ATP),and served as precursors for synthesizing primary products such as polysacc... Purines are building blocks for DNA and RNA,found as the energy currency of cells(ATP and GTP),used as signaling molecules(cGMP,cAMP and ATP),and served as precursors for synthesizing primary products such as polysaccharides,sucrose,and phospholipids as well as secondary products(Stasolla et al.,2003;Pareek et al.,2020).Thus,the synthesis of purines is a critical pathway in the cells of all living organisms.Purines can be synthesized through two pathways:de novo and salvage(Stasolla et al.,2003).Recent investigations revealed that purine synthesis is vital for the proper development of chloroplasts in plants.In Arabidopsis,CIA1 encodes the enzyme glutamine phosphoribosyl pyrophosphate amidotransferase,which catalyzes the first committed step of purine de novo biosynthesis,the loss-of-function mutant cia1 shows small,pale-green mosaic leaves(Hung et al.,2004).In rice,both VAL1 and GARS encode glycinamide ribonucleotide synthetase that mediates the second step in purine biosynthesis. 展开更多
关键词 inosine monophosphate dehydrogenase signaling molecules cgmpcamp gene chloroplast development secondary products stasolla Chinese cabbage Bryl primary products
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Integration of metabolome and transcriptome reveals flavonoid metabolic network during the developmental stage of flower buds in Cornus wilsoniana 认领 引用
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作者 Jiaqi Cai Haoyu Chao +1 位作者 Ming Chen Dijun Chen 《Medicinal Plant Biology》 2025年第1期276-288,共13页
Secondary metabolites(SMs)are crucial for plant adaptation and human health,yet the regulatory mechanisms underlying their biosynthesis remain incompletely understood.Cornus wilsoniana,a woody oil crop and medicinal p... Secondary metabolites(SMs)are crucial for plant adaptation and human health,yet the regulatory mechanisms underlying their biosynthesis remain incompletely understood.Cornus wilsoniana,a woody oil crop and medicinal plant,is renowned for its rich flavonoid content,yet the genetic and metabolic basis of its secondary metabolism remains largely unexplored.Here,we integrate transcriptomic and metabolomic analyses to dissect the regulatory landscape governing flavonoid biosynthesis during flower bud development.Comparative analyses between high-yield and low-yield genotypes across distinct developmental stages reveal a coordinated reprogramming of flavonoid pathways,with significant shifts in gene expression and metabolite accumulation.WGCNA links critical biosynthetic genes to co-expression modules enriched in hormone signaling,redox homeostasis,and light perception,underscoring the interplay between developmental and environmental cues in shaping metabolic fluxes.Furthermore,comparative genomics reveals 17 candidate genes forming a complete anthocyanin and proanthocyanidin biosynthetic network.Our findings illuminate the molecular framework underlying flavonoid metabolism in C.wilsoniana,providing a foundation for metabolic engineering and genetic improvement strategies aimed at enhancing bioactive compound production for medicinal and agricultural applications. 展开更多
关键词 transcriptome woody oil crop secondary metabolism flavonoid metabolic network secondary metabolites sms transcriptomic metabolomic analyses metabolome flower bud development
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Teacher Professionalism and Professional Development of Chinese Secondary Teachers 认领 引用
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作者 何亚平 叶瑛 曹海鑫 《海外英语》 2011年第14期359-363,共5页
The paper refers to some classic texts on professionalism/professionalization which offer a spectrum of different interpretations and shed light on the possible confusion of the terms,with critique on the classic func... The paper refers to some classic texts on professionalism/professionalization which offer a spectrum of different interpretations and shed light on the possible confusion of the terms,with critique on the classic functionalist claims and the emergence of new professionalism.The paper illustrates the history of Chinese secondary teachers over the last few decades,and tries to examine the implications of western conception of teacher professionalism and the professional development of Chinese secondary teachers.With the current trend toward globalization,Chinese teachers professionalism will take on a look of global professionalism(dominated by western professionalism),while with China's own context playing a role in its development. 展开更多
关键词 Chinese secondary teachers teacher professionalism professional development globalization
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Simulation Analysis Module of High-speed Rail Bearings Based on Secondary Development in ADAMS 认领 引用 被引量：4
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《Computer Aided Drafting,Design and Manufacturing》 2013年第3期46-50,共5页
This paper develops a strong secondary development based on ADAMS feature which creates high-speed rail bearings for simulation analysis module. This thesis is in the case of non-circular pattern instructions of how t... This paper develops a strong secondary development based on ADAMS feature which creates high-speed rail bearings for simulation analysis module. This thesis is in the case of non-circular pattern instructions of how to achieve rapid roller modeling, with analysis of functions and parameters required for the design of the simulation module of the high-speed rail bearing, as well as the design of dialog boxes, the environment and file structure. The specific modules is based on the secondary development language provided by ADAMS ! View. Through the menus, dialog boxes which input parameters, it can achieve high iron bearing automatic modeling, dynamic analysis and post-processing to simplify the analysis of high-speed rail bearing operations, as well as improving the high-speed rail bearing development efficiency. 展开更多
关键词 ADAMS secondary development high-speed rail bearing simulation analysis
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China’s Digital Development 认领 引用
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作者 ARASSY EP RAZAFINDRATOVOLAHY SARAH 《ChinAfrica》 2025年第12期58-59,共2页
Technology is transforming China’s economy,governance and quality of life What fascinates me most about China’s digital development is how deeply it has transformed the economy,not only in cities,but across farms,fa... Technology is transforming China’s economy,governance and quality of life What fascinates me most about China’s digital development is how deeply it has transformed the economy,not only in cities,but across farms,factories,and daily life.Digitalisation here is not a privilege of the elite but a nationwide strategy applied to the primary,secondary,and tertiary sectors with purpose and precision. 展开更多
关键词 quality life governance secondary sector economy digital development transformation nationwide strategy primary sector
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Research on the Professional Development Mode of Primary and Secondary School English Teachers 认领 引用
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作者 Lijun Wang Danxia Liu 《International Journal of Technology Management》 2016年第5期47-49,共3页
In this paper, we conduct research on the professional development mode of primary and the secondary school English teachers. Teacher training is an effective way to promote the teachers’ professional development tha... In this paper, we conduct research on the professional development mode of primary and the secondary school English teachers. Teacher training is an effective way to promote the teachers’ professional development that is an important content of teacher education, and teachers’ pre-service training, entry education together constitute a complete content of teacher education, teacher training, of course and is also the need of teachers’ lifelong learning, for the national government attached great importance to teachers’ training work. English teaching is a kind of creative and complicated work, and whole teaching activity involves teachers, students, teaching objective and the teaching content, teaching means and teaching links such as basic elements. This paper proposes the novel perspective of the professional development mode for teachers that will be meaningful. 展开更多
关键词 Development Mode Primary and Secondary English Teachers Literature Review
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A Study on the Professional Development Strategies of English Teachers in Rural Primary and Secondary Schools 认领 引用
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作者 Yuling Zuo 《管理科学与研究（中英文版）》 2019年第1期31-34,共4页
In the new round of development, the government should not only focus on the development of large enterprises, but also on small enterprises that can grow explosively. According to the growth characteristics of unicor... In the new round of development, the government should not only focus on the development of large enterprises, but also on small enterprises that can grow explosively. According to the growth characteristics of unicorn enterprises, the "Guidelines for the Cultivation of High-Growth Enterprises" should be formulated at the provincial level. On the basis of the clear definition of highgrowth enterprises, the classification criteria are defined. A set of government support mechanisms for enterprise selection, approval and support should be established. In addition, a set of positive support policies should be adopted for different types of enterprises in land use, financing, taxation, technology, talent recruitment and other aspects. 展开更多
关键词 Rural primary and secondary schools English teachers Professional development problems and countermeasures
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Development of EFL Students' Writing in Secondary Education 认领 引用
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作者 Ana Cristina Lahuerta Martinez Alberto Isusi Alabarte 《Journal of Linguistics and Education Research》 2018年第1期1-13,共13页
The aim of this paper is to explore and measure language learners'performance in L2 writing production using the complexity,accuracy,and fluency constructs.A total of 123 secondary education students took part in ... The aim of this paper is to explore and measure language learners'performance in L2 writing production using the complexity,accuracy,and fluency constructs.A total of 123 secondary education students took part in the study.Results are manifold.In the first place,they show that the measures of fluency,accuracy,grammatical and lexical complexity progress in a significant way:fourth grade students outperform first graders in the aforementioned measures.Secondly,fewer correlations between the writing measures used and the general quality of the compositions are found among the older students than among the younger ones,indicating that the correlations change depending on learners'age.Thirdly,1st year students exhibit a higher ratio of errors,both in general and also by error category,although only two types decrease significantly in 4th year students:syntactic and spelling errors.Lastly,we find that errors tend to develop in a non-linear way. 展开更多
关键词 L2 writing development Accuracy Fluency Grammatical complexity Lexical complexity Secondary education
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The Effective Development of Moral Education Work of Secondary Vocational Class Teachers 认领 引用
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作者 LIUYilin 《外文科技期刊数据库（文摘版）教育科学》 2022年第2期093-096,共4页
In the current education work, the most concerned about the moral education, because it has a strong influence, if we still stay in the traditional concept and standards, not only can not achieve ideal results, but al... In the current education work, the most concerned about the moral education, because it has a strong influence, if we still stay in the traditional concept and standards, not only can not achieve ideal results, but also may encounter many new problems. Objectively speaking, in carrying out moral work in the process of the secondary vocational school, the teacher in charge, to see their own problems, form the accurate cognition, take scientific and reasonable teaching means, strictly implement the global education to the task, and in the correct concept, standards organization daily teaching, the comprehensive progress of full preparation for the future. This paper mainly discusses the effective content of moral education work in secondary vocational school teachers, and puts forward some targeted measures, hoping to play a good help. 展开更多
关键词 secondary teacher moral education effective development exploration and discussion
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The Composition and Promotion Strategy of Teachers'Curriculum Development Ability in Secondary Vocational Schools 认领 引用
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作者 Yingji Li 《Journal of Contemporary Educational Research》 2020年第11期79-83,共5页
The curriculum development ability of secondary vocational school teachers is a necessary professional ability for teachers.It has important practical significance and practical value for promoting secondary vocationa... The curriculum development ability of secondary vocational school teachers is a necessary professional ability for teachers.It has important practical significance and practical value for promoting secondary vocational education curriculum and teaching reform.On the basis of analyzing the connotation and composition of curriculum development ability of teachers in secondary vocational schools,this article proposes three strategies to improve the curriculum development ability of teachers in secondary vocational schools. 展开更多
关键词 Secondary vocational school teacher Ability curriculum development Composition Strategy
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The Way of the Novice English Teachers' Professional Development in Secondary School 认领 引用
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作者 YANG Manli 《International English Education Research》 2016年第4期55-57,共3页
In the modern times, the professional development of teachers is a hot topic among the educational world. Facilitating the professional development of teachers is one of a most important way to improve the teachers＇ ... In the modern times, the professional development of teachers is a hot topic among the educational world. Facilitating the professional development of teachers is one of a most important way to improve the teachers＇ personalities. However, how to facilitate the professional development of the novice English teacher in the secondary school is still a big and headache problem in front of all education managers and all novice English teachers. For this reason, it has a huge practical significance to look for some ways to research the professional development of the novice English teachers in secondary school. The objective of this research is to promote the professional development of novice English teachers in secondary school through a wide range of ways. It also aims to promote the whole personalities of all English teachers and the whole English teaching qualities. This paper wiU research in how to promote and facilitate the professional development of the novice English teachers in secondary school from the parts of country, school and teachers. Specificly speaking, from the perspective of country, it contains the way of some short trains for novice teachers from superior educational department and some educational action research; From the perspective of the secondary school, it includes school-based in-service training, the exploitation of school-based curriculum and learning team; From the perspective of teacher themselves, it includes teaching reflection, observing other teachers＇ class and using micro-teaching to promote teachers＇ teaching skills. 展开更多
关键词 secondary school novice English teachers the professional development
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Design Practice of Green Campus in Primary and Secondary Schools Based on Sustainable Development 认领 引用
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作者 WANGXiaoyang 《外文科技期刊数据库（文摘版）工程技术》 2022年第6期104-107,共4页
In the current architectural design concept, sustainable development has become the mainstream of architectural design, and this architectural idea has gradually extended to the campus. The design of green campus has ... In the current architectural design concept, sustainable development has become the mainstream of architectural design, and this architectural idea has gradually extended to the campus. The design of green campus has attracted more and more attention in recent years, and the exploration of green campus construction in China is at the initial stage, so there are still many problems in the overall practice, especially in the design of green campus in primary and secondary schools. In the green campus design of primary and secondary schools, it is necessary to carry out green design of campus buildings on the basis of meeting the basic functional requirements of the campus, and at the same time, aiming at the universal problems in the design, adopt corresponding strategies to solve them. 展开更多
关键词 sustainable development primary and secondary schools campus design green campus
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Research on the evaluation index system of development work of the transformation of the secondary school of newly-built local undergraduate colleges and universities in our country 认领 引用
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作者 ZHAO Yinggang 《International English Education Research》 2016年第5期65-67,共3页
The newly-built local undergraduate colleges and universities positively respond to the call of national policy and successively joined the rank of transition to the application of technology-based development of univ... The newly-built local undergraduate colleges and universities positively respond to the call of national policy and successively joined the rank of transition to the application of technology-based development of university, in order to seek their own development path. Whether the transformation development work of secondary college can succeed or not directly decide whether the transformation work of the whole college can carry on smoothly or not. This essay, by establishing eighteen secondary indicators, investigates seven aspects of the newly-built local undergraduate colleges and universities secondary college transformation work, such as the general idea, specialty construction, talents training mode, teaching staff construction, integration of production with teaching, social services and quality assurance, hoping that it can play a reference to the work of other colleges and universities. 展开更多
关键词 The newly-built local undergraduate colleges and universities Secondary college The transformation development work Assessment index system
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Rapid Modeling of an Excavator Working Device Based on the Secondary Development of Pro/E 认领 引用
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作者 CHENG Jie-jie WANG Chun-yan +2 位作者 WANG Hui BAI Peng-wei MA Xiao-fang 《International Journal of Plant Engineering and Management》 2012年第3期173-177,共5页
In order to shorten the design cycle of the excavator working device, we have proposed a rapid modeling method for the excavator working device which uses parameters. Based on the Prooolkit, which is secondary develop... In order to shorten the design cycle of the excavator working device, we have proposed a rapid modeling method for the excavator working device which uses parameters. Based on the Prooolkit, which is secondary development tool of Pro/E4.0,and combined with Vs C＋＋2005 programming software. It developed a flexible set of MFC visualization-friendly interfaces. Users can enter data in the visual interface according to their needs and it will generate a new part model quickly. So it improves the design quality, shortens the design cycle, and makes the cost lower significantly. 展开更多
关键词 excavator working device rapid modeling parameters Prooolkit secondary development Pro/engineer
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CsTs,a C-type lectin receptor-like kinase,regulates the development trichome development and cuticle metabolism in cucumber(Cucumis sativus) 认领 引用 被引量：1
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作者 Duo Lv HaiFan Wen +10 位作者 Gang Wang Juan Liu ChunLi Guo Jingxian Sun Keyan Zhang ChaoHan Li Jiaqi You Ming Pan Huanle He Run Cai Junsong Pan 《Horticulture Research》 SCIE CSCD 2024年第10期251-262,共12页
Cucumber(Cucumis sativus)fruit spines are a classic material for researching the development of multicellular trichomes.Some key genes that influence trichome development have been confirmed to be associated with cuti... Cucumber(Cucumis sativus)fruit spines are a classic material for researching the development of multicellular trichomes.Some key genes that influence trichome development have been confirmed to be associated with cuticle biosynthesis and secondary metabolism.However,the biological mechanisms underlying trichome development,cuticle biosynthesis,and secondary metabolism in cucumber remain poorly understood.CsTs,a C-type lectin receptor-like kinase gene,reportedly causes a tender trichome phenotype in cucumber when it mutates.In this study,the role of CsTs in cucumber fruit spines morphogenesis was confirmed using gene editing technology.Sectioning and cell wall component detection were used to analyse the main reason of tender fruit spines in the ts mutant.Subsequently,transcriptome data and a series of molecular biology experiments were used to further investigate the relationship between CsTs and cytoskeletal homeostasis in cucumber.CsTs overexpression partially compensated for the abnormal trichome phenotype of an Arabidopsis homolog mutant.Genetic hybridization and metabolic analysis indicated that CsTs and CsMict can affect trichome development and cuticle biosynthesis in the same pathway.Our findings provide important background information for further researching on the molecular mechanism underlying cucumber trichome development and contribute to understanding the biological function of C-type lectin receptor-like kinases. 展开更多
关键词 C type lectin receptor kinase development multicellular trichomessome secondary metabolism cucumber cuticle biosynthesis CSTS secondary metabolismhoweverthe Cucumis sativus
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