Exploring the geological genesis of selenium(Se)in Se-rich soil of the Hetao Plain,a major grain production base of corn,wheat,potato,sunflower and zucchini in northern China,is vital for the rational development of S...Exploring the geological genesis of selenium(Se)in Se-rich soil of the Hetao Plain,a major grain production base of corn,wheat,potato,sunflower and zucchini in northern China,is vital for the rational development of Se-rich land resources and the environmental geochemical assessment.In this study,element geochemical characterization,geospatial analysis and mathematical statistics were employed to investigate the factors influencing the spatial distribution of soil Se in the Hetao Plain,focusing on the differences in soil chemical weathering intensity,rare earth elements distribution pattern and soil sedimentary sequence characteristics between the study area and typical surrounding parent materials.The results indicated that the soil Se content in the study area ranged from 0.07 mg/kg to 0.72 mg/kg,with an average value of 0.24 mg/kg,which was higher than the regional background values.Moreover,this suggested that local Se enrichment occurred in the soil of study area,and that the Se distribution was restricted by factors including soil clay minerals,constant elements and soil organic matter(SOM)contents.The characteristics of soil parent material in the study area differed significantly from those of the piedmont diluvial parent material,whereas were similar to those of the Yellow River floodplain and lacustrine parent materials,revealing that the soil parent material in the study area mainly came from the water system transportation.Through combining the characteristics of soil sedimentary sequences in the study area with the location of the Yellow River ancient channel,it is suggested that the origin of high Se soil in the central and northern belt zone of the study area is associated with the Yellow River ancient channel,where the enrichment pattern is“channel sedimentary type”.In addition,the development and utilization potential of Se-rich cultivated land was explored based on the biological effects of Se.It was concluded that the Se content of crops(corn,sunflower and zucchini)in the study area was higher than that of similar crops in China,that crop species and soil Se content were the dominant influencing factors of crop Se content in the study area,and that the crops growing in Se-rich areas had higher Se enrichment rate than those growing in non-Se-rich areas.This exhibited a high development and utilization potential of the investigated cultivated land for Se-rich crop production.展开更多
In recent years,the incidence of chronic inflammatory bowel disease(IBD)has been increasing,posing a serious threat to human health.Medication can lead to off-target,systemic side effects and serious complications,and...In recent years,the incidence of chronic inflammatory bowel disease(IBD)has been increasing,posing a serious threat to human health.Medication can lead to off-target,systemic side effects and serious complications,and safer treatments are urgently needed.Selenium(Se)supplementation can alleviate IBD by modulating gut flora and increasing antioxidant enzyme activities.Glutathione peroxidase(GPx)is a selenoprotein with good antioxidant capacity to eliminate inflammation and protect the intestinal.Herein,this paper comprehensively reviews the recent research progresses of Se-containing GPxs(GPx1-4)in alleviating IBD with oxidation stress as the main thread,summaries the underlying mechanisms of Se-containing GPx on the regulation of IBD.The different effects of inorganic Se,organic Se,and Se nanoparticles in the treatment of IBD are discussed.展开更多
Selenium(Se),an essential trace element,plays a critical role in protecting the toxicity of methylmercury(MeHg).However,its detoxification mechanism for alleviating MeHg-induced damage remains largely unexplored.This ...Selenium(Se),an essential trace element,plays a critical role in protecting the toxicity of methylmercury(MeHg).However,its detoxification mechanism for alleviating MeHg-induced damage remains largely unexplored.This study focused on the antagonistic effects of Se supplementation on the toxic responses induced by MeHg pretreatment in Caenorhabditis elegans.Our results showed that following a 20 h pre-exposure to MeHg,4 h exposure to Se effectively and rapidly antagonized the reproductive and neurological impairments induced by MeHg.Meanwhile,we found that the total Hg content decreased from 166±46.0 to 109±18.7µg/g after the addition of Se.Apart from inhibiting the bioaccumulation of Hg,Se supplementation reduced MeHg-induced reactive oxygen species(ROS)and promoted mitochondrial fusion to improve mitochondrial quality.In addition,MeHg-induced autophagy could be alleviated by increasing lysosome activity after the addition of Se.Further studies revealed that Se supplementation modulated the expression of gss-1 and gst-4,regulating glutathione(GSH)synthesis and elevated MeHg-decreased GSH content from 45.5%to 79.7%.These findings suggested that Se recovered MeHg-induced reproductive and neurological damage by modulating mitochondrial function and GSH synthesis,providing valuable insights for developing novel therapeutic strategies against MeHg toxicity.展开更多
As an essential micronutrient,selenium(Se)plays crucial roles in maintaining cutaneous homeostasis through multifaceted mechanisms including redox regulation,immunomodulation,and anti-tumorigenic activity.While epidem...As an essential micronutrient,selenium(Se)plays crucial roles in maintaining cutaneous homeostasis through multifaceted mechanisms including redox regulation,immunomodulation,and anti-tumorigenic activity.While epidemiological and preclinical studies substantiate the therapeutic promise of Se in managing dermatological pathologies such as psoriasis,atopic dermatitis,and cutaneous malignancies,conventional Se formulations face translational challenges due to suboptimal bioavailability and doselimiting hepatotoxicity.Recent advancements in nanobiotechnology have catalyzed the emergence of Se nanoparticles(Se NPs)as next-generation therapeutic platforms.These engineered nanostructures exhibit superior pharmacokinetic profiles characterized by enhanced epithelial permeability,stimuli-responsive drug release kinetics,and targeted biodistribution.This comprehensive review systematically examines:(1)pathophysiological barriers in dermatologic therapy;(2)Se-mediated molecular circuitry;(3)nanoenabled theranostic breakthroughs;(4)preclinical validation of Se NPs-based combinatorial regimens.By critically evaluating structure-activity relationships of various nanoformulations,we delineate a translational roadmap bridging nanomaterial design principles with clinical needs in precision dermatology.This synthesis aims to accelerate the clinical deployment of Se nanotechnology while addressing key regulatory and manufacturing challenges.展开更多
Copper(Cu)contamination impairs crop performance.Selenium(Se),a beneficial element for plants,has been implicated in mitigating heavy-metal stress.However,the role of Se against Cu toxicity in tobacco(Nicotiana tabacu...Copper(Cu)contamination impairs crop performance.Selenium(Se),a beneficial element for plants,has been implicated in mitigating heavy-metal stress.However,the role of Se against Cu toxicity in tobacco(Nicotiana tabacum L.)remains incompletely characterized.Using Cu-stressed tobacco seedlings with Se supplementation,we show that excess Cu disrupted chloroplast structure,perturbed photorespiration,and interfered with chlorophyll biosynthesis and disrupted the Calvin–Benson cycle(including Ribulose-1,5-bisphosphate(RuBP)regeneration),thereby reducing photosynthetic efficiency.Se preserved chloroplast integrity and enhanced pigment synthesis,improving leaf photosynthetic performance.Se application also significantly decreased soil available Cu,which lowered plant Cu uptake and translocation,while concurrently promoting mineral nutrient acquisition.Moreover,Se modulated antioxidant defenses to mitigate oxidative damage and maintain cellular structure and function.At the metabolic level,Se appeared to confer Cu tolerance through regulation of glutathione metabolism and amino-acid pathways(notably histidine,arginine,and proline),accompanied by changes in glutamate,glutathione,and phosphoserine.Collectively,this study suggested that Se might alleviate Cu phytotoxicity through multiple,concerted pathways—including lowering soil-available Cu,reducing plant Cu uptake,safeguarding chloroplast/photosynthetic processes,and modulating antioxidant and amino-acid metabolism.展开更多
Low-valence-selenium-substituted glucose,a novel organoselenium compound,has emerged as a promising candidate with significant biological application potential.This review highlights its role as an efficient and low-t...Low-valence-selenium-substituted glucose,a novel organoselenium compound,has emerged as a promising candidate with significant biological application potential.This review highlights its role as an efficient and low-toxicity selenium source in agriculture,livestock farming,and medicine.In agriculture,it enhances selenium uptake in crops,improves plant stress resistance,and reduces heavy metal toxicity.In animal feed,it boosts selenium levels and antioxidant capacity in livestock,thereby improving their health and productivity.Additionally,its medical applications include the development of selenium catalysts for pharmaceutical synthesis and selenium-enriched materials with antibacterial and anticancer properties.This article provides a comprehensive review of its synthesis,biological efficacy,and future prospects.展开更多
Geometrical configurations at the nanometer scale are inherently linked to electronic properties,offering exciting opportunity to engineer the latter through precise structural control.The honeycomb structure,a promin...Geometrical configurations at the nanometer scale are inherently linked to electronic properties,offering exciting opportunity to engineer the latter through precise structural control.The honeycomb structure,a prominent geometry in two-dimensional materials like graphene,has become a versatile platform for advancing energy technologies,quantum computing,and nanoscale sensing.Achieving a perfect honeycomb network at large scale remains challenging but desired,especially when atomic defects and disorder can severely impact materials'properties and performances.Intrinsic topological defects often persist due to the conformational flexibility of the precursor skeletons,which allows precursor monomers to deform despite variations in preparation parameters.To address this challenge,we employ a tripod molecular precursor,p TBPT,combined with ultrahigh vacuum on-surface synthesis.Networks comprising rings of different edges are initially formed after deposition of p TBPT on Cu(111)at room temperature to 420 K.At low coverage(~0.015 monolayer)selenium doping,we achieve the fabrication of ordered honeycomb networks with much improved structural homogeneity.Selenium doping facilitated the formation of ordered two-dimensional metal-organic nanostructure from 360 K to 480 K.The disorder-order transition of molecular networks through selenium doping on Cu(111)is explored through high-resolution scanning tunneling microscopy(STM).A persistent homology method is resorted to quantify the degree of order of our patterns.The regulation of energy diagrams in the absence or presence of the selenium atom is revealed by density functional theory(DFT)calculations.These findings can enrich the on-surface synthesis toolbox of conformationally flexible precursors,for the design of ordered nanoarchitectures,and for future development of engineered honeycomb nanomaterials.展开更多
Selenium(Se)serves as a trace element essential for the human body owing to its significant physiological functions and extensive pharmacological effects.The Se required by the human body is primarily obtained from so...Selenium(Se)serves as a trace element essential for the human body owing to its significant physiological functions and extensive pharmacological effects.The Se required by the human body is primarily obtained from soil-derived foods.This study revealed Se-rich soils covering a certain area and Se-rich edible wild mushrooms with high Se accumulation rates in Chuxiong,central Yunnan Province,China through a geochemical survey of soil quality.Furthermore,this study investigated the Se migration and transformation mechanisms in the soil-wild mushroom system,aiming to provide a scientific basis for the development and planning of Se-rich green foods in the study area.Using the geochemical data of samples collected from topsoils,deep soils,and wild mushrooms and their root soils in Nanhua County,Chuxiong,this study analyzed the Se contents in soils and wild mushrooms and their root soils and explored the mechanisms and influencing factors of Se enrichment in wild mushrooms.The results indicate that the topsoils in the study area exhibit Se contents ranging from 0.07 mg/kg to 0.95 mg/kg,with an arithmetic average of 0.25 mg/kg.The Se-rich soils cover an area of 356 km2,which accounts for 13.07% of the total topsoil area.The wild mushrooms in the study area display Se contents varying from 0.004 mg/kg to 47.10 mg/kg,with a median of 0.977 mg/kg.The analyses of Moran’s index and semivariogram indicate that the Se content distributions in both topsoils and deep soils in the study area exhibit distinct spatial structures.Specifically,the semivariogram model for the Se content in the deep soils emerges as a Gaussian model,and the Se content exhibits a nugget-to-sill ratio of 21.72%,suggesting that the Se content in deep soils is primarily influenced by structural factors such as parent materials.Se in the soils originates primarily from soil-forming parent rocks,with the origin of the Se-rich soils closely related to Triassic black shales,thin coal seams,and metamorphic rocks in the Ailao Mountain area.The wild mushrooms in the study area enjoy significantly higher Se content than other reported naturally Se-rich agricultural products,with a Se accumulation rate of up to 92.31%and an overall over-limit ratio of Pb and Cd of merely 11.54%,suggesting that the study area has substantial potential for the development of naturally Se-rich green foods.The wild mushrooms in the study area exhibit bioconcentration factors(BCFs)of Se ranging from 0.02 to 157.00(median:4.26),with Se bioavailability decreasing in the order of Boletus edulis,Boletus aereus,Leccinum nigrescens,Ramaria botrytoides,and Russula virescens.For the Se absorption and enrichment in the wild mushrooms,the primary controlling factor is identified as the wild mushroom species.Furthermore,they are significantly influenced by the Se content in soils but are minimally affected by the physicochemical indicators of soils.展开更多
The article presents the study of the effect of nanocomposites(NCs)based on selenium(Se),copper(Cu),and manganese(Mn)nanoparticles(NPs)embedded in a matrix of natural polysaccharides—arabinogalactan(AG),carrageenan(C...The article presents the study of the effect of nanocomposites(NCs)based on selenium(Se),copper(Cu),and manganese(Mn)nanoparticles(NPs)embedded in a matrix of natural polysaccharides—arabinogalactan(AG),carrageenan(CAR),and starch(ST)—on the content of chlorophylls(Chls)and carotenoids in potato tissues in vitro.Potatoes were grown for 28 days on Murashige-Skoog(MS)medium with the addition of a NC,then pigments were isolated from leaf tissues,and their content was determined spectrophotometrically.Both a stimulating effect and an inhibitory effect of different NCs on the pigment content were found.Se and Cu NCs in the AG matrices(Se/AG and Cu/AG NCs)increased the Chl content both in the leaf tissues of healthy potato plants and plants infected with the phytopathogenic bacterium Clavibacter sepedonicus(Cms).Mn NCs increased the content of carotenoids and Chls in potatoes infected with Cms.A decrease in the pigment content was detected when growing potatoes on a medium with the addition of Cu NC based on a ST matrix(Cu/ST NC),which is probably due to the high content of NPs in this NC(20%).It was concluded that NCs based on an AG matrix are capable of exerting a stimulating effect on the content of potato pigments,which can be used to increase the yield of this crop.展开更多
Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation a...Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.展开更多
The implementation of multifunctional application scenarios for mobile terminal devices has increased the energy density requirements of batteries.Increasing the charging voltage can rapidly increase the specific capa...The implementation of multifunctional application scenarios for mobile terminal devices has increased the energy density requirements of batteries.Increasing the charging voltage can rapidly increase the specific capacity of layered transition metal oxides;however,it also exacerbates the release of lattice oxygen and the contraction of the unit cell.Ternary materials are designed in a secondary particle state to meet the requirements of power battery applications.Therefore,to create ternary materials that can operate under ultrahigh voltages,attention should be given to both surface modification and particle integrity maintenance.By utilizing elemental selenium(Se)with a low melting point,easy sublimation,and multiple variable valence states,deep grain boundary modification was implemented inside the particles.The performance of the cathode material was evaluated through pouch cells,and the improvement mechanism was explored through molecular dynamics simulation calculations.Under the protection of a three-dimensional Se-rich modified layer,LiNi1/3Co1/3Mn1/3O2achieved stable operation at ultrahigh voltages(4.6 V vs.Li/Li+);a sacrificial protection mechanism based on the chronic decomposition of the Se-rich layer was proposed to explain the efficacy of Se modification in stabilizing ternary materials.This deep grain boundary modification based on elemental Se provides a new solution for the ultrahigh-voltage operation of transition metal oxides and provides a scientific basis and technical support for solving the interface contact problem of all-solid-state batteries.展开更多
Gray blight caused by Pseudopestalotiopsis sp.,is a major foliar disease that significantly reduces both yield and quality of tea[Camellia sinensis(L.)Kuntze].Although the combined application of selenium(Se)and melat...Gray blight caused by Pseudopestalotiopsis sp.,is a major foliar disease that significantly reduces both yield and quality of tea[Camellia sinensis(L.)Kuntze].Although the combined application of selenium(Se)and melatonin(MT)has been shown to effectively mitigate this disease,the underlying mechanisms by which the resistance is enhanced in tea plants remain poorly understood.In the present study,we investigated the inhibitory effects of Se and MT on Ps.camelliae-sinensis and its role in enhancing disease resistance of‘Shuchazao’tea plant.The results showed that Se significantly inhibited mycelial growth,with an EC50 of 5.80μg·mL-1,while MT alone had no significant effect.However,the combination of Se and MT(5.8 mg·L-1+200μmol·L-1)exerted a stronger inhibitory effect on the fungal growth.In vivo experiments demonstrated that Se and MT reduced the lesion diameter by 66.84%and decreased disease severity by 75.8%in tea plants.Transcriptomic and metabolomic analyses revealed that Se and MT regulated secondary metabolite biosynthesis,particularly that of triterpenoids and flavonoids,which are critical for disease resistance.Specifically,Se and MT treatment reduced the accumulation of tenadhesion-related triterpenoids and significantly increased the production of the antifungal compounds Tangeretin and Cinchonain Ib∗.These findings suggest the synergistic effects of Se and MT in controlling tea gray blight through direct pathogen inhibition and the induction of defense systems in the host plant via the regulation of higher bioactive compounds and signaling pathways.The combined application of Se and MT offers a promising alternative to chemical fungicides for managing gray blight disease of tea,while also enabling MT-biofortified and/or Se-enriched green tea.展开更多
为解决某高校教务系统处理流程重复度高、操作频繁、人工效率低和人工出错率高等问题,对Web应用的机器人流程自动化RPA(Robotic Process Automation)进行研究分析,设计教务系统登录、课表查询和成绩录入等功能模块的RPA解决方案,引入钉...为解决某高校教务系统处理流程重复度高、操作频繁、人工效率低和人工出错率高等问题,对Web应用的机器人流程自动化RPA(Robotic Process Automation)进行研究分析,设计教务系统登录、课表查询和成绩录入等功能模块的RPA解决方案,引入钉钉机器人、Selenium、SpringBoot框架和OCR等技术来实现RPA.在某高校教务系统的实验验证表明,机器人节省教师手工操作时间最小70.14%,最大值可达94.53%,对越复杂的任务机器人操作优势越显著.机器人操作准确率接近100%,可以连续不间断工作,减少人力成本,为其它Web应用的RPA提供参考价值,也为企业数字化升级和流程变革提供新的思路.展开更多
Selenium-enriched tea is highly valued for its nutritional and health benefits due to its rich selenium content and various bioactive compounds,such as tea polyphenols,theanine,and catechins.However,the limited availa...Selenium-enriched tea is highly valued for its nutritional and health benefits due to its rich selenium content and various bioactive compounds,such as tea polyphenols,theanine,and catechins.However,the limited availability of selenium resources in soil significantly impedes the development of the seleniumenriched tea industry.Selenium-reducing bacteria,as functional microorganisms capable of efficiently converting inorganic selenium into selenium nanoparticles(SeNPs),play a crucial role in selenium enrichment and stabilization processes in tea plants.In this study,a highly selenium-tolerant strain,Raoultella ornithinolytica S-1,was successfully isolated,demonstrating an exceptional tolerance to sodium selenite concentrations of up to 200 mM.This strain not only efficiently reduces sodium selenite to SeNPs,but also exhibits multiple growth-promoting functions,including nitrogen fixation,phosphorus solubilization,siderophore production,and IAA synthesis.Furthermore,the S-1 bio-nano selenium microbial agent was successfully prepared using mannitol as the carbon source,tryptone as the nitrogen source,and calcium chloride as the inorganic salt at 5 mM sodium selenite and 4%inoculum.Foliar spraying of the microbial agent significantly increased selenium content in the summer-autumn tea fresh leaves,enhancing the levels of refreshing taste compounds,such as soluble sugars,theanine,and other free amino acids.Concurrently,it reduced the content of bitter substances(tea polyphenols and catechins)and harmful elements(aluminium,cadmium,and fluorine),thereby improving the quality and safety of the summer-autumn tea.This study expands the application of selenium-enriched microorganisms and provides an innovative solution for the development of selenium-enriched tea.展开更多
Background Selenium(Se)is an essential soil mineral that can be incorporated into animal feedstuffs.Because of a lack of soil Se in some regions,organic or inorganic supplementation strategies must be implemented to p...Background Selenium(Se)is an essential soil mineral that can be incorporated into animal feedstuffs.Because of a lack of soil Se in some regions,organic or inorganic supplementation strategies must be implemented to prevent deficiencies and promote optimal ovine health.Therefore,the objectives of this study were to assess how inorganic versus organic Se supplementation influenced ewe Se status and immune function during late gestation and postpartum.Dorset Rideau ewes(n=110)were fed a Se deficient diet from gestation d 110 through postpartum d 49 and received one of four daily oral Se treatments diluted in 5 mL of sugar water:0 mg Se,0.3 mg inorganic Se,0.3 mg organic Se,and 0.6 mg organic Se.Throughout the trial,the ewes received various immune challenges,including intramuscular immunizations with a novel antigen(ovalbumin;OVA)on trial d 0 and 10,an intradermal OVA challenge on d 20,and a lipopolysaccharide(LPS)endotoxin challenge on trial d 49.Results The organic Se treatment groups had higher serum Se concentrations on most trial days compared to the 0.3 mg inorganic and control groups(P0.05).However,4 h post-LPS injection,the serum albumin concentration was significantly lower in the 0.3 mg inorganic group compared to both organic Se groups,potentially indicating a higher degree of inflammation in the ewes supplemented with the inorganic Se.Conclusions The results of this study indicate that organic Se supplementation can promote a higher Se status in ewes over time,but Se supplementation during this study period did not affect tested immunological parameters.This lack of difference in immune responsiveness between groups may be due to an absence of true serum Se deficiencies in the Se-deficient group or the levels of Se supplementation being insufficient to significantly improve immunocompetence.展开更多
Bisphenol A(BPA)is an industrial pollutant that can cause immune impairment.Selenium acts as an antioxidant,as selenium deficiency often accompanies oxidative stress,resulting in organ damage.This study is the first t...Bisphenol A(BPA)is an industrial pollutant that can cause immune impairment.Selenium acts as an antioxidant,as selenium deficiency often accompanies oxidative stress,resulting in organ damage.This study is the first to demonstrate that BPA and/or selenium deficiency induce pyroptosis and ferroptosis-mediated thymic injury in chicken and chicken lymphoma cell(MDCC-MSB-1)via oxidative stress-induced endoplasmic reticulum(ER)stress.We established a broiler chicken model of BPA and/or selenium deficiency exposure and collected thymus samples as research subjects after 42 days.The results demonstrated that BPA or selenium deficiency led to a decrease in antioxidant enzyme activities(T-AOC,CAT,and GSH-Px),accumulation of peroxides(H2O2 and MDA),significant upregulation of ER stress-relatedmarkers(GRP78,IER 1,PERK,EIF-2α,ATF4,and CHOP),a significant increase in iron ion levels,significant upregulation of pyroptosis-related gene(NLRP3,ASC,Caspase1,GSDMD,IL-18 and IL-1β),significantly increase ferroptosis-related genes(TFRC,COX2)and downregulate GPX4,HO-1,FTH,NADPH.In vitro experiments conducted in MDCC-MSB-1 cells confirmed the results,demonstrating that the addition of antioxidant(NAC),ER stress inhibitor(TUDCA)and pyroptosis inhibitor(Vx765)alleviated oxidative stress,endoplasmic reticulum stress,pyroptosis,and ferroptosis.Overall,this study concludes that the combined effects of oxidative stress and ER stress mediate pyroptosis and ferroptosis in chicken thymus induced by BPA exposure and selenium deficiency.展开更多
Selenium is one of the important trace elements in the human body.Its deficiency will directly affect human health.With people's attention to health,the content of selenium in food has gradually attracted attentio...Selenium is one of the important trace elements in the human body.Its deficiency will directly affect human health.With people's attention to health,the content of selenium in food has gradually attracted attention.However,detecting selenium compounds in complex samples remains a challenge.In this work,we built an online heating-reaction device.This device combines the electrospray extraction ionization mass spectrometry(EESI-MS)with the heating reaction device,which can simultaneously detect various selenium compounds in complex liquid samples.Under acidic conditions,the sample was heated and catalyzed by a heating reaction device,so that the SeO~(2-)3and O-phenylenediamine(OPD)could generate 1,3-dihydro-2,1,3-benzoselenadiazole.Based on the above reactions,we can detect organic selenium,inorganic selenium and other compounds in liquid samples by organic mass spectrometry.In this experiment,we determined the content of three forms of selenium:selenomethionine(SeMet),l-selenocystine(SeCys(2)),and sodium selenite.The calibration curves for SeMet,SeCys(2),and sodium selenite showed strong linearity within a range of 0.50-50.00μg/L.The limits of detection(LOD)for the three compounds were 0.22,0.27,and 0.41μg/L,respectively.The limits of quantification(LOQ)were 0.68,0.81,and 1.23μg/L,respectively.Spiked recoveries at three levels ranged from 98.8%to 106.1%.In addition,this method can simultaneously detect three selenium compounds and three other specific chemical components in tea infusion samples,providing a rapid and efficient method for identifying tea quality.展开更多
The effects of long-term moisture changes on the migration,release,and bioavailability of selenium in soil are complex.Due to the lack of effective monitoring methods for precise quantification,its dynamic behavior is...The effects of long-term moisture changes on the migration,release,and bioavailability of selenium in soil are complex.Due to the lack of effective monitoring methods for precise quantification,its dynamic behavior is still unclear.Based on the DGT(Diffusive Gradients in Thin-films)technology,this study sets up three moisture control scenarios:continuous wet,wet-dry alternating,and continuous dry,and carries out a 6-month soil moisture control experiment.In the experiment,the DGT device collected the diffusion gradient data of soil selenium under different scenarios,and analyzed the migration characteristics of selenium in combination with the adsorption isotherm.Meanwhile,the release rate,migration coefficient,and bioavailability parameters of selenium are calculated by fitting the first-order kinetic model,further verifying the reliability and applicability of the DGT data.The experimental results demonstrate that under continuous wet conditions,the release rate of soil selenium reaches 1.85µg·cm-2·h-1,with a migration coefficient of 0.012 cm2·h-1and a bioavailability parameter of 0.74;under wet-dry alternating conditions,they are 1.42µg·cm-2·h-1,0.01 cm2·h-1,and 0.68,respectively;under continuous dry conditions,the release rate of soil selenium is the smallest,at 0.88µg·cm-2·h-1,with a migration coefficient of 0.004 cm-2·h-1and a bioavailability parameter of 0.5.The results of this experiment reveal the dynamic behavior of soil selenium under different moisture conditions and reflect the high efficiency of DGT technology in dynamic monitoring and quantitative analysis of soil selenium behavior,providing a scientific basis for the optimal management of rhizosphere soil selenium.展开更多
Wound dressings with tissue adhesion,good mechanical,antioxidant and anti-inflammatory performance are urgently needed.In this work,we present a multifunctional selenium nanoparticles(SeNPs)/citric acid/gelatin/hydrox...Wound dressings with tissue adhesion,good mechanical,antioxidant and anti-inflammatory performance are urgently needed.In this work,we present a multifunctional selenium nanoparticles(SeNPs)/citric acid/gelatin/hydroxysuccinimide-grafted polyacrylic acid nanocomposite hydrogel adhesive(SCA) specifically designed for wound healing applications.The SCA was prepared via a one-pot processing,where SeNPs synthesized via chemical reduction were incorporated.These SeNPs not only endowed SCA with robust wet adhesion ability,excellent stretchability,and skin-matched elasticity modulus by serving as a physical crosslinker to modulate swelling equilibrium and molecular slippage,but also enhanced the biocompatibility and free radical scavenging capacity of SCA.Furthermore,in vivo evaluation of full-thickness cutaneous defects of rats revealed that SCA effectively reduced inflammation,promoted wound closure,and increased collagen deposition.All these results demonstrated that the developed SCA offers a promising therapeutic strategy for wound healing applications.展开更多
基金financially funded by Science and Technology Innovation Foundation of Comprehensive Survey & Command Center for Natural Resources (KC20220006)the projects of China Geological Survey (DD20243000, DD20191015)
摘要Exploring the geological genesis of selenium(Se)in Se-rich soil of the Hetao Plain,a major grain production base of corn,wheat,potato,sunflower and zucchini in northern China,is vital for the rational development of Se-rich land resources and the environmental geochemical assessment.In this study,element geochemical characterization,geospatial analysis and mathematical statistics were employed to investigate the factors influencing the spatial distribution of soil Se in the Hetao Plain,focusing on the differences in soil chemical weathering intensity,rare earth elements distribution pattern and soil sedimentary sequence characteristics between the study area and typical surrounding parent materials.The results indicated that the soil Se content in the study area ranged from 0.07 mg/kg to 0.72 mg/kg,with an average value of 0.24 mg/kg,which was higher than the regional background values.Moreover,this suggested that local Se enrichment occurred in the soil of study area,and that the Se distribution was restricted by factors including soil clay minerals,constant elements and soil organic matter(SOM)contents.The characteristics of soil parent material in the study area differed significantly from those of the piedmont diluvial parent material,whereas were similar to those of the Yellow River floodplain and lacustrine parent materials,revealing that the soil parent material in the study area mainly came from the water system transportation.Through combining the characteristics of soil sedimentary sequences in the study area with the location of the Yellow River ancient channel,it is suggested that the origin of high Se soil in the central and northern belt zone of the study area is associated with the Yellow River ancient channel,where the enrichment pattern is“channel sedimentary type”.In addition,the development and utilization potential of Se-rich cultivated land was explored based on the biological effects of Se.It was concluded that the Se content of crops(corn,sunflower and zucchini)in the study area was higher than that of similar crops in China,that crop species and soil Se content were the dominant influencing factors of crop Se content in the study area,and that the crops growing in Se-rich areas had higher Se enrichment rate than those growing in non-Se-rich areas.This exhibited a high development and utilization potential of the investigated cultivated land for Se-rich crop production.
基金supported by the National Natural Science Foundation of China General Project(31972050)Guangxi Natural Science Foundation(2023GXNSFDA026044).
摘要In recent years,the incidence of chronic inflammatory bowel disease(IBD)has been increasing,posing a serious threat to human health.Medication can lead to off-target,systemic side effects and serious complications,and safer treatments are urgently needed.Selenium(Se)supplementation can alleviate IBD by modulating gut flora and increasing antioxidant enzyme activities.Glutathione peroxidase(GPx)is a selenoprotein with good antioxidant capacity to eliminate inflammation and protect the intestinal.Herein,this paper comprehensively reviews the recent research progresses of Se-containing GPxs(GPx1-4)in alleviating IBD with oxidation stress as the main thread,summaries the underlying mechanisms of Se-containing GPx on the regulation of IBD.The different effects of inorganic Se,organic Se,and Se nanoparticles in the treatment of IBD are discussed.
基金supported by the National Key Research and Development Program of China(No.2023YFC3708303)Anhui Provincial Natural Science Foundation(No.2308085UM08)the National Natural Science Foundation of China(No.22376200).
摘要Selenium(Se),an essential trace element,plays a critical role in protecting the toxicity of methylmercury(MeHg).However,its detoxification mechanism for alleviating MeHg-induced damage remains largely unexplored.This study focused on the antagonistic effects of Se supplementation on the toxic responses induced by MeHg pretreatment in Caenorhabditis elegans.Our results showed that following a 20 h pre-exposure to MeHg,4 h exposure to Se effectively and rapidly antagonized the reproductive and neurological impairments induced by MeHg.Meanwhile,we found that the total Hg content decreased from 166±46.0 to 109±18.7µg/g after the addition of Se.Apart from inhibiting the bioaccumulation of Hg,Se supplementation reduced MeHg-induced reactive oxygen species(ROS)and promoted mitochondrial fusion to improve mitochondrial quality.In addition,MeHg-induced autophagy could be alleviated by increasing lysosome activity after the addition of Se.Further studies revealed that Se supplementation modulated the expression of gss-1 and gst-4,regulating glutathione(GSH)synthesis and elevated MeHg-decreased GSH content from 45.5%to 79.7%.These findings suggested that Se recovered MeHg-induced reproductive and neurological damage by modulating mitochondrial function and GSH synthesis,providing valuable insights for developing novel therapeutic strategies against MeHg toxicity.
基金provided by the General Program of Natural Science Foundation of Guangdong Province(No.2023A1515010015)Educational Reform Research Project on Resident Standardized Training at the Dermatology Hospital of Southern Medical University(Nos.ZP202401,ZP202404)Science and Technology Project for Social Development in Dongguan City(No.20221800905452)。
摘要As an essential micronutrient,selenium(Se)plays crucial roles in maintaining cutaneous homeostasis through multifaceted mechanisms including redox regulation,immunomodulation,and anti-tumorigenic activity.While epidemiological and preclinical studies substantiate the therapeutic promise of Se in managing dermatological pathologies such as psoriasis,atopic dermatitis,and cutaneous malignancies,conventional Se formulations face translational challenges due to suboptimal bioavailability and doselimiting hepatotoxicity.Recent advancements in nanobiotechnology have catalyzed the emergence of Se nanoparticles(Se NPs)as next-generation therapeutic platforms.These engineered nanostructures exhibit superior pharmacokinetic profiles characterized by enhanced epithelial permeability,stimuli-responsive drug release kinetics,and targeted biodistribution.This comprehensive review systematically examines:(1)pathophysiological barriers in dermatologic therapy;(2)Se-mediated molecular circuitry;(3)nanoenabled theranostic breakthroughs;(4)preclinical validation of Se NPs-based combinatorial regimens.By critically evaluating structure-activity relationships of various nanoformulations,we delineate a translational roadmap bridging nanomaterial design principles with clinical needs in precision dermatology.This synthesis aims to accelerate the clinical deployment of Se nanotechnology while addressing key regulatory and manufacturing challenges.
基金This work was supported by the Projects of Science and Technology Department of Henan Province(No.212102110445)the Natural Science Foundation of Henan Province(No.222300420176)the Talents Project of Henan Agriculture University(Nos.30500846 and 30500999).
摘要Copper(Cu)contamination impairs crop performance.Selenium(Se),a beneficial element for plants,has been implicated in mitigating heavy-metal stress.However,the role of Se against Cu toxicity in tobacco(Nicotiana tabacum L.)remains incompletely characterized.Using Cu-stressed tobacco seedlings with Se supplementation,we show that excess Cu disrupted chloroplast structure,perturbed photorespiration,and interfered with chlorophyll biosynthesis and disrupted the Calvin–Benson cycle(including Ribulose-1,5-bisphosphate(RuBP)regeneration),thereby reducing photosynthetic efficiency.Se preserved chloroplast integrity and enhanced pigment synthesis,improving leaf photosynthetic performance.Se application also significantly decreased soil available Cu,which lowered plant Cu uptake and translocation,while concurrently promoting mineral nutrient acquisition.Moreover,Se modulated antioxidant defenses to mitigate oxidative damage and maintain cellular structure and function.At the metabolic level,Se appeared to confer Cu tolerance through regulation of glutathione metabolism and amino-acid pathways(notably histidine,arginine,and proline),accompanied by changes in glutamate,glutathione,and phosphoserine.Collectively,this study suggested that Se might alleviate Cu phytotoxicity through multiple,concerted pathways—including lowering soil-available Cu,reducing plant Cu uptake,safeguarding chloroplast/photosynthetic processes,and modulating antioxidant and amino-acid metabolism.
基金the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Yangzhou University)(No.SJCX25_2352)Yangzhou Key Research and Development Program:Industry Foresight and Key Core Technology(No.YZ2023019)+1 种基金Cooperation Project of Yangzhou City with Yangzhou University(No.YZ2023209)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)for financial support.
摘要Low-valence-selenium-substituted glucose,a novel organoselenium compound,has emerged as a promising candidate with significant biological application potential.This review highlights its role as an efficient and low-toxicity selenium source in agriculture,livestock farming,and medicine.In agriculture,it enhances selenium uptake in crops,improves plant stress resistance,and reduces heavy metal toxicity.In animal feed,it boosts selenium levels and antioxidant capacity in livestock,thereby improving their health and productivity.Additionally,its medical applications include the development of selenium catalysts for pharmaceutical synthesis and selenium-enriched materials with antibacterial and anticancer properties.This article provides a comprehensive review of its synthesis,biological efficacy,and future prospects.
基金financial support from the National Research Foundation Singapore(No.NRF-CRP26-2021RS0002)。
摘要Geometrical configurations at the nanometer scale are inherently linked to electronic properties,offering exciting opportunity to engineer the latter through precise structural control.The honeycomb structure,a prominent geometry in two-dimensional materials like graphene,has become a versatile platform for advancing energy technologies,quantum computing,and nanoscale sensing.Achieving a perfect honeycomb network at large scale remains challenging but desired,especially when atomic defects and disorder can severely impact materials'properties and performances.Intrinsic topological defects often persist due to the conformational flexibility of the precursor skeletons,which allows precursor monomers to deform despite variations in preparation parameters.To address this challenge,we employ a tripod molecular precursor,p TBPT,combined with ultrahigh vacuum on-surface synthesis.Networks comprising rings of different edges are initially formed after deposition of p TBPT on Cu(111)at room temperature to 420 K.At low coverage(~0.015 monolayer)selenium doping,we achieve the fabrication of ordered honeycomb networks with much improved structural homogeneity.Selenium doping facilitated the formation of ordered two-dimensional metal-organic nanostructure from 360 K to 480 K.The disorder-order transition of molecular networks through selenium doping on Cu(111)is explored through high-resolution scanning tunneling microscopy(STM).A persistent homology method is resorted to quantify the degree of order of our patterns.The regulation of energy diagrams in the absence or presence of the selenium atom is revealed by density functional theory(DFT)calculations.These findings can enrich the on-surface synthesis toolbox of conformationally flexible precursors,for the design of ordered nanoarchitectures,and for future development of engineered honeycomb nanomaterials.
基金supported by the projects of the China Geological Survey (DD20220987, DD20242954, and ZD20220211)
摘要Selenium(Se)serves as a trace element essential for the human body owing to its significant physiological functions and extensive pharmacological effects.The Se required by the human body is primarily obtained from soil-derived foods.This study revealed Se-rich soils covering a certain area and Se-rich edible wild mushrooms with high Se accumulation rates in Chuxiong,central Yunnan Province,China through a geochemical survey of soil quality.Furthermore,this study investigated the Se migration and transformation mechanisms in the soil-wild mushroom system,aiming to provide a scientific basis for the development and planning of Se-rich green foods in the study area.Using the geochemical data of samples collected from topsoils,deep soils,and wild mushrooms and their root soils in Nanhua County,Chuxiong,this study analyzed the Se contents in soils and wild mushrooms and their root soils and explored the mechanisms and influencing factors of Se enrichment in wild mushrooms.The results indicate that the topsoils in the study area exhibit Se contents ranging from 0.07 mg/kg to 0.95 mg/kg,with an arithmetic average of 0.25 mg/kg.The Se-rich soils cover an area of 356 km2,which accounts for 13.07% of the total topsoil area.The wild mushrooms in the study area display Se contents varying from 0.004 mg/kg to 47.10 mg/kg,with a median of 0.977 mg/kg.The analyses of Moran’s index and semivariogram indicate that the Se content distributions in both topsoils and deep soils in the study area exhibit distinct spatial structures.Specifically,the semivariogram model for the Se content in the deep soils emerges as a Gaussian model,and the Se content exhibits a nugget-to-sill ratio of 21.72%,suggesting that the Se content in deep soils is primarily influenced by structural factors such as parent materials.Se in the soils originates primarily from soil-forming parent rocks,with the origin of the Se-rich soils closely related to Triassic black shales,thin coal seams,and metamorphic rocks in the Ailao Mountain area.The wild mushrooms in the study area enjoy significantly higher Se content than other reported naturally Se-rich agricultural products,with a Se accumulation rate of up to 92.31%and an overall over-limit ratio of Pb and Cd of merely 11.54%,suggesting that the study area has substantial potential for the development of naturally Se-rich green foods.The wild mushrooms in the study area exhibit bioconcentration factors(BCFs)of Se ranging from 0.02 to 157.00(median:4.26),with Se bioavailability decreasing in the order of Boletus edulis,Boletus aereus,Leccinum nigrescens,Ramaria botrytoides,and Russula virescens.For the Se absorption and enrichment in the wild mushrooms,the primary controlling factor is identified as the wild mushroom species.Furthermore,they are significantly influenced by the Se content in soils but are minimally affected by the physicochemical indicators of soils.
基金supported by the federal program№0277-2021-0004(121031300011-7)of the Ministry of Science and Higher Education of the Russian Federation with the basic project“Study of molecular mechanisms of physiological processes and allelopathy in plant-microbe relationships”.
摘要The article presents the study of the effect of nanocomposites(NCs)based on selenium(Se),copper(Cu),and manganese(Mn)nanoparticles(NPs)embedded in a matrix of natural polysaccharides—arabinogalactan(AG),carrageenan(CAR),and starch(ST)—on the content of chlorophylls(Chls)and carotenoids in potato tissues in vitro.Potatoes were grown for 28 days on Murashige-Skoog(MS)medium with the addition of a NC,then pigments were isolated from leaf tissues,and their content was determined spectrophotometrically.Both a stimulating effect and an inhibitory effect of different NCs on the pigment content were found.Se and Cu NCs in the AG matrices(Se/AG and Cu/AG NCs)increased the Chl content both in the leaf tissues of healthy potato plants and plants infected with the phytopathogenic bacterium Clavibacter sepedonicus(Cms).Mn NCs increased the content of carotenoids and Chls in potatoes infected with Cms.A decrease in the pigment content was detected when growing potatoes on a medium with the addition of Cu NC based on a ST matrix(Cu/ST NC),which is probably due to the high content of NPs in this NC(20%).It was concluded that NCs based on an AG matrix are capable of exerting a stimulating effect on the content of potato pigments,which can be used to increase the yield of this crop.
基金supported by the National Natural Science Foundation of China(32302774)Beijing Innovation Consortium of Agriculture Research System(BAIC04)+1 种基金China Agriculture Research System(CARS-40)the Agricultural Science and Technology Innovation Program of the Feed Research Institute of the Chinese Academy of Agricultural Sciences(CAASIFR-ZDRW202402).
摘要Background Organic selenium(Se)has gained recognition in poultry nutrition as a feed additive to boost production and Se deposition in eggs and tissues,owing to its high bioavailability,efficient tissue accumulation and minimal toxicity.Selenium-enriched yeast(SeY)is a well-established source,while selenium-enriched lactobacilli(SeL),a newer alternative,offers the added benefits of probiotics.This study examined the effects of SeY and SeL on egg quality,antioxidant capacity,Se deposition,and gut health in laying hens.After a two-week pre-treatment with a Sedeficient diet(SeD),450 Hy-Line Brown laying hens(30-week-old)were assigned into five dietary groups with six replicates of 15 hens each.The groups included a SeD,SeD supplemented with 1.5 mg Se/kg from SeY(SeY15),or 1.5,3.0,and 6.0 mg Se/kg from SeL(SeL15,SeL30,SeL60).The feeding trial lasted for 12 weeks.Results SeY15 and SeL15 improved the feed-to-egg ratio(P<0.05)in the latter stages.Haugh units were significantly increased(P<0.05)in the SeY15 and SeL30 groups,while darker yolk color(P<0.05)was observed in the SeY15,SeL15,and SeL60 groups.All Se-supplemented diets increased Se content in whole eggs,albumen,and yolk(P<0.05),while SeL groups showed a dose-dependent effect.Antioxidant enzyme activities increased,and MDA content decreased in the serum(P<0.05),with SeY15 showing the highest GSH-Px levels(P<0.05).SeL60 increased serum alkaline phosphatase and aspartate transaminase,and distorted the liver architecture(P<0.05).Se-diets reduced concentrations of reactive oxygen species(ROS)in the ileum and liver(P<0.05).SeL15 improved the ileal villus height-tocrypt depth ratio(P<0.05).SeY15 and/or SeL15 up-regulated TXNRD1 and SEPHS1 mRNA while down-regulating SCLY expression in the liver.SeY15 altered ileal microbiota by increasing both beneficial and pathogenic bacteria,whereas SeL15 predominantly boosted beneficial bacteria.Conclusion SeL integrates the antioxidant properties of organic Se with the probiotic benefits on gut health,resulting in a performance-enhancing effect comparable to that of SeY.However,high SeL level(6.0 mg Se/kg)compromised productivity and metabolic functions while enhancing Se deposition.
基金supported by the National Natural Science Foundation of China (52302259)the China Postdoctoral Science Foundation (CPSF) under Grant Number 2023M741479+4 种基金the Postdoctoral Fellowship Program of CPSF under Grant Number GZB20240280the Jiangxi Provincial Natural Science Foundation (20224ACB218006)the financial support from High-level Talent Research Special Funds of Jiangxi University of Science and Technology (Grant No. 205200100670)the Jiangxi Provincial Key Laboratory of Power Energy Storage Batteries and Materials (2024SSY10011)the Major Scientific and Technological Research R&D Special Project of Jiangxi Province(20244AFI92002)
摘要The implementation of multifunctional application scenarios for mobile terminal devices has increased the energy density requirements of batteries.Increasing the charging voltage can rapidly increase the specific capacity of layered transition metal oxides;however,it also exacerbates the release of lattice oxygen and the contraction of the unit cell.Ternary materials are designed in a secondary particle state to meet the requirements of power battery applications.Therefore,to create ternary materials that can operate under ultrahigh voltages,attention should be given to both surface modification and particle integrity maintenance.By utilizing elemental selenium(Se)with a low melting point,easy sublimation,and multiple variable valence states,deep grain boundary modification was implemented inside the particles.The performance of the cathode material was evaluated through pouch cells,and the improvement mechanism was explored through molecular dynamics simulation calculations.Under the protection of a three-dimensional Se-rich modified layer,LiNi1/3Co1/3Mn1/3O2achieved stable operation at ultrahigh voltages(4.6 V vs.Li/Li+);a sacrificial protection mechanism based on the chronic decomposition of the Se-rich layer was proposed to explain the efficacy of Se modification in stabilizing ternary materials.This deep grain boundary modification based on elemental Se provides a new solution for the ultrahigh-voltage operation of transition metal oxides and provides a scientific basis and technical support for solving the interface contact problem of all-solid-state batteries.
基金supported by the National Key Research and Development Program of China(Grant Nos.2024YFD2001100,2024YFD2001105,2017YFD0201600)the National Natural Science Foundation of China(Grant No.31401545)+1 种基金the Natural Science Foundation of Anhui Province(Grant No.1408085MC68)the Natural Science Fund of Education Department of Anhui Province(Grant No.KJ2017A158).
摘要Gray blight caused by Pseudopestalotiopsis sp.,is a major foliar disease that significantly reduces both yield and quality of tea[Camellia sinensis(L.)Kuntze].Although the combined application of selenium(Se)and melatonin(MT)has been shown to effectively mitigate this disease,the underlying mechanisms by which the resistance is enhanced in tea plants remain poorly understood.In the present study,we investigated the inhibitory effects of Se and MT on Ps.camelliae-sinensis and its role in enhancing disease resistance of‘Shuchazao’tea plant.The results showed that Se significantly inhibited mycelial growth,with an EC50 of 5.80μg·mL-1,while MT alone had no significant effect.However,the combination of Se and MT(5.8 mg·L-1+200μmol·L-1)exerted a stronger inhibitory effect on the fungal growth.In vivo experiments demonstrated that Se and MT reduced the lesion diameter by 66.84%and decreased disease severity by 75.8%in tea plants.Transcriptomic and metabolomic analyses revealed that Se and MT regulated secondary metabolite biosynthesis,particularly that of triterpenoids and flavonoids,which are critical for disease resistance.Specifically,Se and MT treatment reduced the accumulation of tenadhesion-related triterpenoids and significantly increased the production of the antifungal compounds Tangeretin and Cinchonain Ib∗.These findings suggest the synergistic effects of Se and MT in controlling tea gray blight through direct pathogen inhibition and the induction of defense systems in the host plant via the regulation of higher bioactive compounds and signaling pathways.The combined application of Se and MT offers a promising alternative to chemical fungicides for managing gray blight disease of tea,while also enabling MT-biofortified and/or Se-enriched green tea.
摘要为解决某高校教务系统处理流程重复度高、操作频繁、人工效率低和人工出错率高等问题,对Web应用的机器人流程自动化RPA(Robotic Process Automation)进行研究分析,设计教务系统登录、课表查询和成绩录入等功能模块的RPA解决方案,引入钉钉机器人、Selenium、SpringBoot框架和OCR等技术来实现RPA.在某高校教务系统的实验验证表明,机器人节省教师手工操作时间最小70.14%,最大值可达94.53%,对越复杂的任务机器人操作优势越显著.机器人操作准确率接近100%,可以连续不间断工作,减少人力成本,为其它Web应用的RPA提供参考价值,也为企业数字化升级和流程变革提供新的思路.
基金supported by the research and developmentprogram of China Se-enriched industry research institute(Grant No.2024FXZX-01)the Key Research and Development Program ofShaanxi(Grant No.2024NC-ZDCYL-04-07 and 2025NC-YBXM-240)the China Agricul-tural Research System of the Ministry of Finance and the Ministry ofAgriculture and Rural Affairs(CARS-19).
摘要Selenium-enriched tea is highly valued for its nutritional and health benefits due to its rich selenium content and various bioactive compounds,such as tea polyphenols,theanine,and catechins.However,the limited availability of selenium resources in soil significantly impedes the development of the seleniumenriched tea industry.Selenium-reducing bacteria,as functional microorganisms capable of efficiently converting inorganic selenium into selenium nanoparticles(SeNPs),play a crucial role in selenium enrichment and stabilization processes in tea plants.In this study,a highly selenium-tolerant strain,Raoultella ornithinolytica S-1,was successfully isolated,demonstrating an exceptional tolerance to sodium selenite concentrations of up to 200 mM.This strain not only efficiently reduces sodium selenite to SeNPs,but also exhibits multiple growth-promoting functions,including nitrogen fixation,phosphorus solubilization,siderophore production,and IAA synthesis.Furthermore,the S-1 bio-nano selenium microbial agent was successfully prepared using mannitol as the carbon source,tryptone as the nitrogen source,and calcium chloride as the inorganic salt at 5 mM sodium selenite and 4%inoculum.Foliar spraying of the microbial agent significantly increased selenium content in the summer-autumn tea fresh leaves,enhancing the levels of refreshing taste compounds,such as soluble sugars,theanine,and other free amino acids.Concurrently,it reduced the content of bitter substances(tea polyphenols and catechins)and harmful elements(aluminium,cadmium,and fluorine),thereby improving the quality and safety of the summer-autumn tea.This study expands the application of selenium-enriched microorganisms and provides an innovative solution for the development of selenium-enriched tea.
基金Ontario Ministry of Agriculture,Food and Agribusiness(OMAFA)Natural Sciences and Engineering Research Council of Canada(NSERC)+1 种基金AdisseoOntario Sheep Farmers。
摘要Background Selenium(Se)is an essential soil mineral that can be incorporated into animal feedstuffs.Because of a lack of soil Se in some regions,organic or inorganic supplementation strategies must be implemented to prevent deficiencies and promote optimal ovine health.Therefore,the objectives of this study were to assess how inorganic versus organic Se supplementation influenced ewe Se status and immune function during late gestation and postpartum.Dorset Rideau ewes(n=110)were fed a Se deficient diet from gestation d 110 through postpartum d 49 and received one of four daily oral Se treatments diluted in 5 mL of sugar water:0 mg Se,0.3 mg inorganic Se,0.3 mg organic Se,and 0.6 mg organic Se.Throughout the trial,the ewes received various immune challenges,including intramuscular immunizations with a novel antigen(ovalbumin;OVA)on trial d 0 and 10,an intradermal OVA challenge on d 20,and a lipopolysaccharide(LPS)endotoxin challenge on trial d 49.Results The organic Se treatment groups had higher serum Se concentrations on most trial days compared to the 0.3 mg inorganic and control groups(P0.05).However,4 h post-LPS injection,the serum albumin concentration was significantly lower in the 0.3 mg inorganic group compared to both organic Se groups,potentially indicating a higher degree of inflammation in the ewes supplemented with the inorganic Se.Conclusions The results of this study indicate that organic Se supplementation can promote a higher Se status in ewes over time,but Se supplementation during this study period did not affect tested immunological parameters.This lack of difference in immune responsiveness between groups may be due to an absence of true serum Se deficiencies in the Se-deficient group or the levels of Se supplementation being insufficient to significantly improve immunocompetence.
基金supported by the National Natural Science Foundation of China Regional Joint Innovation Fund (No.U22A20524)the Heilongjiang Province Natural Science Foundation Key projects (No.ZD2023C002).
摘要Bisphenol A(BPA)is an industrial pollutant that can cause immune impairment.Selenium acts as an antioxidant,as selenium deficiency often accompanies oxidative stress,resulting in organ damage.This study is the first to demonstrate that BPA and/or selenium deficiency induce pyroptosis and ferroptosis-mediated thymic injury in chicken and chicken lymphoma cell(MDCC-MSB-1)via oxidative stress-induced endoplasmic reticulum(ER)stress.We established a broiler chicken model of BPA and/or selenium deficiency exposure and collected thymus samples as research subjects after 42 days.The results demonstrated that BPA or selenium deficiency led to a decrease in antioxidant enzyme activities(T-AOC,CAT,and GSH-Px),accumulation of peroxides(H2O2 and MDA),significant upregulation of ER stress-relatedmarkers(GRP78,IER 1,PERK,EIF-2α,ATF4,and CHOP),a significant increase in iron ion levels,significant upregulation of pyroptosis-related gene(NLRP3,ASC,Caspase1,GSDMD,IL-18 and IL-1β),significantly increase ferroptosis-related genes(TFRC,COX2)and downregulate GPX4,HO-1,FTH,NADPH.In vitro experiments conducted in MDCC-MSB-1 cells confirmed the results,demonstrating that the addition of antioxidant(NAC),ER stress inhibitor(TUDCA)and pyroptosis inhibitor(Vx765)alleviated oxidative stress,endoplasmic reticulum stress,pyroptosis,and ferroptosis.Overall,this study concludes that the combined effects of oxidative stress and ER stress mediate pyroptosis and ferroptosis in chicken thymus induced by BPA exposure and selenium deficiency.
基金financially supported by Jiangxi University of Chinese Medicine School-level Science and Technology Innovation Team Development Program(No.CXTD22005)PhD research startup fund of Jiangxi University of Chinese Medicine(No.2023BSZR005)。
摘要Selenium is one of the important trace elements in the human body.Its deficiency will directly affect human health.With people's attention to health,the content of selenium in food has gradually attracted attention.However,detecting selenium compounds in complex samples remains a challenge.In this work,we built an online heating-reaction device.This device combines the electrospray extraction ionization mass spectrometry(EESI-MS)with the heating reaction device,which can simultaneously detect various selenium compounds in complex liquid samples.Under acidic conditions,the sample was heated and catalyzed by a heating reaction device,so that the SeO~(2-)3and O-phenylenediamine(OPD)could generate 1,3-dihydro-2,1,3-benzoselenadiazole.Based on the above reactions,we can detect organic selenium,inorganic selenium and other compounds in liquid samples by organic mass spectrometry.In this experiment,we determined the content of three forms of selenium:selenomethionine(SeMet),l-selenocystine(SeCys(2)),and sodium selenite.The calibration curves for SeMet,SeCys(2),and sodium selenite showed strong linearity within a range of 0.50-50.00μg/L.The limits of detection(LOD)for the three compounds were 0.22,0.27,and 0.41μg/L,respectively.The limits of quantification(LOQ)were 0.68,0.81,and 1.23μg/L,respectively.Spiked recoveries at three levels ranged from 98.8%to 106.1%.In addition,this method can simultaneously detect three selenium compounds and three other specific chemical components in tea infusion samples,providing a rapid and efficient method for identifying tea quality.
摘要The effects of long-term moisture changes on the migration,release,and bioavailability of selenium in soil are complex.Due to the lack of effective monitoring methods for precise quantification,its dynamic behavior is still unclear.Based on the DGT(Diffusive Gradients in Thin-films)technology,this study sets up three moisture control scenarios:continuous wet,wet-dry alternating,and continuous dry,and carries out a 6-month soil moisture control experiment.In the experiment,the DGT device collected the diffusion gradient data of soil selenium under different scenarios,and analyzed the migration characteristics of selenium in combination with the adsorption isotherm.Meanwhile,the release rate,migration coefficient,and bioavailability parameters of selenium are calculated by fitting the first-order kinetic model,further verifying the reliability and applicability of the DGT data.The experimental results demonstrate that under continuous wet conditions,the release rate of soil selenium reaches 1.85µg·cm-2·h-1,with a migration coefficient of 0.012 cm2·h-1and a bioavailability parameter of 0.74;under wet-dry alternating conditions,they are 1.42µg·cm-2·h-1,0.01 cm2·h-1,and 0.68,respectively;under continuous dry conditions,the release rate of soil selenium is the smallest,at 0.88µg·cm-2·h-1,with a migration coefficient of 0.004 cm-2·h-1and a bioavailability parameter of 0.5.The results of this experiment reveal the dynamic behavior of soil selenium under different moisture conditions and reflect the high efficiency of DGT technology in dynamic monitoring and quantitative analysis of soil selenium behavior,providing a scientific basis for the optimal management of rhizosphere soil selenium.
基金supported by National Natural Science Foundation of China(No.52403042)China Postdoctoral Science Foundation(No.2023M742472)。
摘要Wound dressings with tissue adhesion,good mechanical,antioxidant and anti-inflammatory performance are urgently needed.In this work,we present a multifunctional selenium nanoparticles(SeNPs)/citric acid/gelatin/hydroxysuccinimide-grafted polyacrylic acid nanocomposite hydrogel adhesive(SCA) specifically designed for wound healing applications.The SCA was prepared via a one-pot processing,where SeNPs synthesized via chemical reduction were incorporated.These SeNPs not only endowed SCA with robust wet adhesion ability,excellent stretchability,and skin-matched elasticity modulus by serving as a physical crosslinker to modulate swelling equilibrium and molecular slippage,but also enhanced the biocompatibility and free radical scavenging capacity of SCA.Furthermore,in vivo evaluation of full-thickness cutaneous defects of rats revealed that SCA effectively reduced inflammation,promoted wound closure,and increased collagen deposition.All these results demonstrated that the developed SCA offers a promising therapeutic strategy for wound healing applications.