期刊文献+
任意字段
题名或关键词
题名
关键词
摘要
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到1,986篇文章
< 1 2 … 100 >
每页显示 20 50 100
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
显示方式：
Combined volatile metabolome and transcriptome analysis of 60 peach cultivars provide new insights into the formation of aroma and the identification of associated genes 认领 引用 被引量：2
1
作者 Wenyi Duan Zhenyu Yao +7 位作者 Chengkui Qiao Junren Meng Shihang Sun Lei Pan Liang Niu Guochao Cui Zhiqiang Wang Wenfang Zeng 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第5期1025-1038,共14页
Volatile organic compounds(VOCs)are the main chemical compounds that determine the characteristic aroma and flavor of fruit.In this study,we identified a total of 97 VOCs,including 5C6 compounds,12 aldehydes,4 alcohol... Volatile organic compounds(VOCs)are the main chemical compounds that determine the characteristic aroma and flavor of fruit.In this study,we identified a total of 97 VOCs,including 5C6 compounds,12 aldehydes,4 alcohols,10 esters,13 lactones,18 terpenes,8 norisoprenoids,8 ketones,3 hydrocarbons,8 phenylalanine derivates,and 8 other compounds,in 60 peach cultivars using headspace solid-phase microextraction(HS-SPME)combined with gas chromatography-mass spectrophotometry(GC-MS).A wide range of VOCs were detected in these germplasm resources with respect to both quantity and concentration.Correlation analysis with different physiological traits demonstrated that the genetic background exerts a significant influence on the composition and content of VOCs among peach cultivars.For example,the content of norisoprenoid was significantly lower in yellow-fleshed peach than in white-fleshed peach,and lactones were almost undetectable in stony hard peach.Among the 97 VOCs,26 exhibited odor activity values(OAVs)exceeding 1,suggesting that these compounds act as key odorants in the peach VOC composition.Moreover,six structural genes associated with the synthesis of γ-decalactone and(Z)-3-hexenyl acetate and five genes linked to aldehyde and 1-octen-3-one biosynthesis were identified through weighted gene co-expression network analysis(WGCNA).Additionally,15 transcription factors(TFs)were identified as potentially regulating VOC synthesis.Overall,these data provide insight into the factors contributing to the differences in aroma qualities among peach cultivars,which can help to promote the development of peach breeding. 展开更多
关键词 Volatile aroma compounds Peach cultivars OAVs Transcriptome
暂未订购 下载PDF
Complete mitochondrial genome map and transcriptome reveal the mechanisms of a novel neau type CMS in Brassica rapa 认领 引用 被引量：1
2
作者 Yan Liu Xingliang Wang +5 位作者 Jiaqi Liu Xianfeng Zhang Wei Luan Jiaxi Li Ran Gu Yaowei Zhang 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第7期1667-1680,共14页
Cytoplasmic male sterility(CMS)is a widespread phenomenon in crops and is beneficial for commercial hybrid breeding.Although CMS systems in Brassica species are extensively utilized,they are derived from relatively li... Cytoplasmic male sterility(CMS)is a widespread phenomenon in crops and is beneficial for commercial hybrid breeding.Although CMS systems in Brassica species are extensively utilized,they are derived from relatively limited sources,and the underlying molecular mechanisms are not well understood.In this study,a novel CMS line,named Neau,was identified through distant hybridization between Matthiola incana and Brassica rapa.Neau CMS exhibits agronomic traits similar to its maintainer line of Chinese cabbage and displays sterility at the tetrad stage.The mitochondrial genomes of Neau CMS and its maintainer line were assembled to 359596 bp and 219771 bp,respectively,encoding 99 and 66 unknown open reading frames(orfs).Six orfs were identified as specific to Neau CMS with sequence alignments,with orf154 and two copies of orf138 identified as candidate genes based on their transmembrane structures and their ability to encode toxic proteins that inhibited Escherichia coli cell growth.The expression of orf154 was induced during the tetrad stage by quantitative real-time polymerase chain reaction(qRT-PCR),whereas orf138a showed higher expression at the microspore mononuclear stage in Neau CMS.Transcriptome analysis revealed reduced expressions of most pollen development-related genes,as well as genes involved in peroxidase/oxidative stress responses and the electron transfer chain in Neau CMS.Additionally,Neau CMS exhibited increased levels of O2.-,H2O2,and malondialdehyde(MDA),alongside reduced activities of SOD,CAT,and POD.Both ATP content and ATPase activity were down-regulated in Neau CMS.Overexpression of orf138 and orf154 in Arabidopsis thaliana resulted in reductions in pollen quantity,viability,and seed production.These findings suggest that orf138 and orf154 are key candidate genes responsible for the sterility observed in Neau CMS in Chinese cabbage. 展开更多
关键词 Distant hybridization CMS Brassica.rapa Transcriptome
暂未订购 下载PDF
Transcriptome and metabolome analysis reveals regulatory network associated with light-independent anthocyanin biosynthesis in blueberry fruit 认领 引用
3
作者 Xuyan Li Shouwen Wang +7 位作者 Hongxue Li Fangyun Qian Haiyang Wang Jingying Wang Lulu Zhai Baosheng Shi Junwei Huo Shaomin Bian 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第3期584-602,共19页
Anthocyanins are vital secondary metabolites contributing to fruit pigmentation and antioxidative properties.While light is a well-known regulator of anthocyanin biosynthesis,the molecular basis of light-independent a... Anthocyanins are vital secondary metabolites contributing to fruit pigmentation and antioxidative properties.While light is a well-known regulator of anthocyanin biosynthesis,the molecular basis of light-independent anthocyanin accumulation remains underexplored.In this study,integrated analysis of metabolome and transcriptome showed that the anthocyanin content in blueberry(Vaccinium corymbosum‘Bluetta’)fruit was slightly decreased by light-impermeable bagging treatment,while anthocyanin biosynthetic genes were transcriptionally inhibited to different levels,suggesting a slight influence of the bagging treatment on anthocyanin accumulation.Further observation showed that fruit bagging did not alter ethylene production but decreased ABA content.Noticeably,two VcMYBA/MYB1s were not transcriptionally altered by the light-impermeable bagging treatment.Consistently,histochemical GUS analysis and pharmacological manipulation suggested light-independent and ethylene-inducible expression of VcMYBA/MYB1.Moreover,WGCNA analysis revealed 3759 genes positively associated with MYBA/MYB1 such as ethylene-associated genes,etc.Additionally,VcbZIP55s and VcCOP1s were activated and inactivated by the bagging treatment,respectively.These findings provided a framework of light-independent anthocyanin biosynthesis in blueberry fruit. 展开更多
关键词 Vaccinium corymbosum Bagging Light-independent regulation Transcriptome Metabolome Anthocyanins MYBA Ethylene signaling
暂未订购 下载PDF
Comparative transcriptome analysis reveals key genes and pathways involved in the development of adventitious roots in tomato 认领 引用
4
作者 Hexuan Wang Xinyi Zhang +8 位作者 Guohao Yang Xinyi Jia Jiayi Gao Haoran Wang Jingbin Jiang Jingfu Li He Zhang Xiangyang Xu Huanhuan Yang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第7期2859-2877,共19页
The evolutionary development of adventitious roots(ARs)in plants enhances their capacity to adapt to various stress conditions.A thorough analysis of the influencing factors in their morphological construction holds s... The evolutionary development of adventitious roots(ARs)in plants enhances their capacity to adapt to various stress conditions.A thorough analysis of the influencing factors in their morphological construction holds significant theoretical value and practical guidance for overcoming rooting obstacles in cuttings,as well as for cultivating superior varieties characterized by broad adaptability and stress resistance.In this study,we investigated the molecular mechanisms underlying the development of ARs in tomato(Solanum lycopersicum)by performing transcriptome sequencing(RNA-seq).We analyzed the transcription profiles of relevant genes in the"Y962"strain,which exhibits spontaneous AR formation,and the"W961"strain,which does not form ARs.Our findings indicate that the AR induction stage represents an active phase of development,during which we identified 1,676 overlapping genes across the three comparison groups,highlighting the most differentially expressed genes.Functional enrichment analysis showed that they were most closely related to response to auxin,and were also dependent on the crosstalk between other hormones and carbohydrates.Furthermore,through the measurement of endogenous auxin levels and the induction tests with exogenous auxin,it was established that the formation of ARs is closely linked to the accumulation and transport of auxin.Notably,the auxin efflux SIPIN3,which was enriched in the auxin response pathway,exhibited significantly high expression during the induction phase of ARs.The slpin3 mutant,generated using the CRISPR/Cas9 editing system,exhibited a significant reduction in the number of ARs,highlighting the close relationship between polar transport regulated by SIPIN3 and auxin-induced AR formation.In summary,this study not only enriches the developmental network of AR formation in tomatoes with a wealth of data but also elucidates the potential mechanisms for promoting AR development by targeting SIPIN3. 展开更多
关键词 tomato adventitious roots transcriptome auxin S/PIN3
暂未订购 下载PDF
Transcriptome-based analysis reveals a role for PpCDF5 in the promotion of anthocyanin accumulation at a low nighttime temperature 认领 引用
5
作者 Shulin Yang Duanni Wang +6 位作者 Yuhao Gao Jiaxin Zhang Xuan Luo Junbei Ni Yuanwen Teng Jun Su Songling Bai 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第2期285-299,共15页
For red pear,the anthocyanin content is a crucial factor determining the fruit skin color,which affects consumer preferences.Low overnight temperatures promote anthocyanin accumulation,but the molecular mechanism resp... For red pear,the anthocyanin content is a crucial factor determining the fruit skin color,which affects consumer preferences.Low overnight temperatures promote anthocyanin accumulation,but the molecular mechanism responsible is unclear.In this study,‘Hongzaosu’pear(Pyrus pyrifolia×Pyrus communis)fruit were treated with a low nighttime temperature(LNT,16℃)or a warm nighttime temperature(WNT,26℃),with sampling conducted within two diurnal cycles.The results showed that LNT promoted anthocyanin accumulation in the fruit skin.The structural anthocyanin biosynthetic genes PpCHS,PpF3H,and PpUFGT exhibited a rhythmic increase in expression at night under LNT.To examine the underlying mechanism,RNA sequencing was conducted using pear calli exposed to LNT and WNT for different durations(24,48,72,or 96 h).Transcriptome analysis revealed 285 differentially expressed genes(DEGs)common to all pairwise comparisons of LNT-and WNT-treated calli of‘Clapp's Favorite’(P.communis)at the sampling time points.KEGG pathway and gene ontology enrichment analyses indicated that the common DEGs were enriched in secondary metabolic processes and phenylpropanoid metabolic processes,which are associated with anthocyanin biosynthesis.The transcription factor PpCDF5,which was responsive to LNT,was selected for further study.Dual-luciferase assays showed that PpCDF5 activated the transcription of anthocyanin biosynthetic genes PpMYB10,PpCHS,PpF3H,PpDFR,PpANS,and PpUFGT.The yeast one-hybrid and EMSA assays demonstrated that PpCDF5 directly binds to the PpF3H promoter,which contains an AAAG motif.Overexpression of PpCDF5 in pear calli and transient overexpression in pear fruit both increased anthocyanin accumulation.The results indicate that PpCDF5 is involved in LNT-induced anthocyanin biosynthesis in pear fruit and provide insights into the molecular regulation of commercial fruit coloration. 展开更多
关键词 Pear Anthocyanin Low nighttime temperature Transcriptome analysis PpCDF5
暂未订购 下载PDF
Integrated transcriptome and metabolome analysis reveals light induction of anthocyanin rapid accumulation in red-fleshed peach after pre-harvest bagging 认领 引用
6
作者 Jia Wei Fengjie He +6 位作者 Kexin Sun Li Wang Yiming Yin Junbei Ni Songling Bai Yuanwen Teng Huijuan Jia 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第3期539-554,共16页
Red-fleshed fruits are valued for their vibrant color and high anthocyanin content.Pre-harvest fruit bagging enhances fruit peel pigmentation,but its effect on flesh coloration remains poorly characterized.This study ... Red-fleshed fruits are valued for their vibrant color and high anthocyanin content.Pre-harvest fruit bagging enhances fruit peel pigmentation,but its effect on flesh coloration remains poorly characterized.This study revealed that removing bags from‘Gengcunyangtao’red-fleshed peach fruits triggers the rapid and uniform accumulation of anthocyanins in the flesh,resulting in anthocyanin levels that exceed those in unbagged fruits.The exposure to light after bag removal triggered significant increases in anthocyanin levels within 24 h.This was accompanied by the rapid upregulation of light-responsive and flavonoid biosynthetic gene expression levels within 6 h.A metabolomic analysis indicated that anthocyanin precursors,especially p-coumaric acid,accumulated before bag removal,thereby increasing substrate availability for rapid anthocyanin synthesis.On the basis of a weighted gene co-expression network analysis,MYB transcription factors,anthocyanin transporters,glutathione S-transferase,and multidrug and toxic compound extrusion(MATE)were identified as key regulators that coordinate precursor storage along with light-induced transcriptional activation.Notably,PpMYB4 binds to the promoter of PpGSTF14 and activates its expression,thereby promoting anthocyanin accumulation.The study findings elucidated the temporal coordination of metabolic priming and light-responsive transcriptional regulation driving rapid anthocyanin biosynthesis,with possible implications for improving peach fruit flesh coloration. 展开更多
关键词 Red-fleshed peach Anthocyanin Pre-harvest fruit bagging Light exposure Transcriptome analysis Metabolome analysis
暂未订购 下载PDF
Elucidating the protective mechanisms of Chenpi against alcoholic liver injury through integrated analysis of biochemical markers,microbiome,and transcriptome 认领 引用
7
作者 Yanfang Liao Zhaoping Pan +5 位作者 Fuhua Fu Mingfang Peng Yanjiao Fu Wenbin Xiao Yang Shan Jiajing Guo 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第4期1920-1933,共14页
Alcoholic liver injury(ALI)has emerged as a significant public health concern,necessitating the urgent identification of medicinal-food resources for its prevention and treatment.As a natural medicinal food resource,C... Alcoholic liver injury(ALI)has emerged as a significant public health concern,necessitating the urgent identification of medicinal-food resources for its prevention and treatment.As a natural medicinal food resource,Chenpi(CHE)is rich in various flavonoid bioactive compounds and exhibits potential antioxidant,anti-inflammatory,and hepatoprotective effects.However,the effect and mechanism of CHE in preventing and improving ALI are not yet known.This study sought to elucidate CHE's hepatoprotective mechanisms in ALI from the biochemical markers,microbiome and transcriptome perspectives.Results showed that CHE ameliorated ALI by improving oxidative stress,inflammatory cytokines and liver function.16S r RNA analysis revealed that CHE alleviated ALI pathological progression primarily by restoring gut microbiota composition.Transcriptomic analysis showed that CHE improved ALI mainly related to MAPK signaling pathway.Pearson correlation analysis revealed that Candidatus_Saccharimonas,Turicibacter,and Clostridium_sensu_stricto_1 play key roles in the process by which CHE ameliorates alcohol-induced inflammation and pathological angiogenesis.These findings revealed that CHE has the potential to be used as a functional food to prevent or improve ALI. 展开更多
关键词 Chenpi Alcoholic liver injury Microbiome Transcriptome
暂未订购 下载PDF
Unraveling the mechanism of Ligilactobacillus salivarius AR612 in response to glucose stress from the insights of genome and transcriptome analysis 认领 引用
8
作者 Yong Yang Xin Song +3 位作者 Guangqiang Wang Yongjun Xia Zhiqiang Xiong Lianzhong Ai 《Food Science and Human Wellness》 SCIE CAS CSCD 2026年第2期687-703,共17页
This study examined the potential response mechanisms of Ligilactobacillus salivarius AR612 to glucose stress through whole-genome and comparative transcriptome analysis.We obtained the basic genome information of L.s... This study examined the potential response mechanisms of Ligilactobacillus salivarius AR612 to glucose stress through whole-genome and comparative transcriptome analysis.We obtained the basic genome information of L.salivarius AR612.The full genome length of L.salivarius AR612 was 1970245 bp,with a GC content of 33.01%and 1894 coding genes.Moreover,we identified many genes associated with genetic adaptations to various stress factors,including temperature,p H,osmotic pressure,bile salts,and oxidative stress.Physiological analysis revealed that the growth and morphology of AR612 changed significantly under glucose stress,with a decrease in the maximum growth and irregular cell morphology.Furthermore,a comparison of transcriptome data indicated that glucose stress induced changes in the number of differential genes.Moreover,AR612 could respond to extracellular glucose stress by changing the expression of genes related to cell morphology,carbohydrate metabolism,amino acid metabolism,fatty acid synthesis,and nucleotide metabolism.This study provides valuable theoretical insights for future research on the adaptation of L.salivarius AR612 to nutritional stress and its application in industrial processes. 展开更多
关键词 Ligilactobacillus salivarius AR612 Whole genome Transcriptome Glucose stress Differentially expressed genes
暂未订购 下载PDF
Molecular basis of glucose homeostasis in the Pacific oyster(Crassostrea gigas):insights from transcriptome and hexokinase phylogeny analysis 认领 引用
9
作者 Zhitong LIU Xiaojing MIAO Fei XU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2026年第3期1176-1186,共11页
Glucose homeostasis is a fundamental physiological process in both vertebrates and invertebrates,yet its regulatory mechanisms in molluscs remain largely unexplored.This study investigates temporal hemolymph glucose d... Glucose homeostasis is a fundamental physiological process in both vertebrates and invertebrates,yet its regulatory mechanisms in molluscs remain largely unexplored.This study investigates temporal hemolymph glucose dynamics and associated molecular responses in the Pacific oyster Crassostrea gigas.Annual monitoring revealed significant seasonal variation in hemolymph glucose concentrations,with post-spawning oysters exhibiting the lowest levels.A glucose injection experiment demonstrated rapid uptake kinetics,followed by a return to baseline,suggesting the presence of efficient metabolic regulation.Transcriptomic analysis on hepatopancreas tissue identified cgHK2-2 as the dominant hexokinase isoform induced by hyperglycemia,while other HK genes(cgHK2 and cgHK2-like)showed tissue-specific but non-inducible expression profiles in the investigated tissue.Furthermore,upregulation of cgPPP1R3B and cgPCSK1 indicate possibly conserved glycogen metabolism and insulinlike signaling pathways.Phylogenetic analysis revealed divergent evolutionary trajectories of hexokinase in protostomes versus chordates,with oysters lacking a clear glucokinase orthologue.These findings highlight key molecular players in oyster glucose metabolism and suggest both conserved and lineagespecific regulatory strategies. 展开更多
关键词 glucose homeostasis hexokinase PPP1R3B PCSK 1 transcriptome analysis seasonal variation
暂未订购 下载PDF
Integrated methylome–transcriptome profiling reveals epigenetic regulation of immune activation pathways and CSN3-associated lactation repression in bovine subclinical mastitis 认领 引用
10
作者 Shraddha Dwivedi Amit Kumar +7 位作者 Ujjwal Kumar De Anuj Chauhan Ravi Kant Agrawal Shivani Khanna Amritanshu Upadhyay Ayushi Singh Prem Chand Devatwal Triveni Dutt 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第4期1908-1926,共19页
Background Subclinical mastitis(SCM)is a major constraint in dairy production and is driven by complex host–pathogen interactions.Although transcriptional responses associated with SCM have been widely investigated,t... Background Subclinical mastitis(SCM)is a major constraint in dairy production and is driven by complex host–pathogen interactions.Although transcriptional responses associated with SCM have been widely investigated,the epigenetic mechanisms that stably regulate these programs remain less well characterized,particularly in crossbred cattle populations.This study aimed to characterize DNA methylation-based regulatory networks by integrating whole-genome methylation and transcriptome data from milk somatic cells of Vrindavani(Bos taurus×Bos indicus)cattle.Whole-genome methylation(n=6)and corresponding transcriptome profiling(n=6)were performed on milk somatic cells from SCM-affected and healthy control cows.Results Differential methylation analysis(q-value<0.05)identified 62,940 differentially methylated cytosines(DMCs),7,706 differentially methylated regions(DMRs),and 6,203 differentially methylated genes(DMGs),with a predominant bias toward hypomethylation in SCM.Integrative analysis using stringent thresholds for both methylation(≥10%)and expression change(|log2 fold change|≥1;P of GMM<0.001)identified 1,407 differentially methylated and expressed genes(DMEGs).Functional enrichment analysis revealed 47 KEGG pathways and 30 Gene Ontology biological process terms(FDR<0.05),primarily associated with immune signaling and inflammatory responses.In contrast,a subset of DMEGs showed methylationassociated repression of lactation-and metabolism-related genes.Selected genes were experimentally validated by qPCR,including upregulation of the inflammatory mediator S100A8 and downregulation of CSN3(κ-casein),a key milk protein gene.Conclusions These findings provide an integrated view of the DNA methylation and transcriptional landscape of SCM in milk somatic cells and demonstrate that epigenetic remodeling is associated with coordinated activation of immune pathways alongside repression of lactation-associated functions.The results contribute to understanding the molecular basis of subclinical mastitis and may inform future efforts toward biomarker development and epigenetically informed strategies for improving disease resilience in dairy cattle. 展开更多
关键词 DNA methylation Epigenomics Immune system Milk proteins Transcriptome
暂未订购 下载PDF
Deep Reinforcement Learning-Driven Multi-Omics Integration for Constructing gtAge:A Novel Aging Clock from the IgG N-Glycome and Blood Transcriptome 认领 引用
11
作者 Yao Xia Syed Mohammed Shamsul Islam +3 位作者 Xingang Li Abdul Baten Xuerui Tan Wei Wang 《Engineering》 SCIE EI CSCD 2026年第2期100-112,共13页
Previous studies have demonstrated that the immunoglobulin G(IgG)N-glycome and transcriptome are potential biochemical signatures of chronological and biological ages,and several aging clocks have been developed.By in... Previous studies have demonstrated that the immunoglobulin G(IgG)N-glycome and transcriptome are potential biochemical signatures of chronological and biological ages,and several aging clocks have been developed.By integrating the IgG N-glycome and transcriptome,we propose a novel aging clock,gtAge.We developed a deep reinforcement learning-based multiomics integration method called AlphaSnake.The results showed that AlphaSnake achieved a predicted coefficient of determination(R2)value of0.853,outperforming the concatenation-based integration method(R2=0.820)The gtAge estimated by AlphaSnake explained up to 85.3%of the variance in chronological age,which was higher than that in age predicted from IgG N-glycome solely(gAge;R2=0.290)and age predicted from transcriptome solely(tAge;R2=0.812).We also found that the delta age-the difference between the predicted age and chronological age-was associated with several age-related phenotypes.Both delta gtAge and tAge were negatively associated with high-density lipoprotein(p=0.02 and p=0.022,respectively),whereas delta gAge was positively correlated with cholesterol(p=0.006),triglyceride(p=0.002),fasting plasma glucose(p=0.014),low-density lipoprotein(p=0.006),and glycated hemoglobin(p=0.039).These findings suggest that gtAge,tAge,and gAge are potential biomarkers for biological age. 展开更多
关键词 Aging clock IgG N-glycome Transcriptome Multiomics integration Deep reinforcement learning Biological age
暂未订购 下载PDF
Population-level analyses reveal contrasting landscapes of gene expression divergence and evolution between male and female transcriptome lineages in the house mouse 认领 引用
12
作者 Kaizong Wei Chen Xie +6 位作者 Xianghui Zhang Aftab Ahmad Lei Duan Mingji Chu Yuanxiao Gao Diethard Tautz Wenyu Zhang 《Journal of Systematics and Evolution》 SCIE CSCD 2026年第3期470-484,共15页
While sex-biased gene expression and its evolutionary dynamics across taxa have been extensively investigated,systematic separate characterization of the evolutionary patterns in transcriptome divergence between male ... While sex-biased gene expression and its evolutionary dynamics across taxa have been extensively investigated,systematic separate characterization of the evolutionary patterns in transcriptome divergence between male and female lineages remains underexplored.Here,we analyze a comprehensive RNA-seq data set from the house mouse complex,spanning multiple organs across subspecies and species,to delineate the evolutionary trajectories of gene expression in males and females in intra-and inter-species contrasts.For both sexes,we find specific gene expression divergence patterns across the surveyed organs,with a particularly high divergence rate at early evolutionary stages of separation.Comparative analysis between sexes demonstrates male reproductive organs,particularly the testis,displaying accelerated evolutionary rates of expression divergence.Strikingly,testicular long non-coding RNA genes show the most pronounced acceleration,with differences emerging already after a few thousand years of population separation.In contrast,somatic organs and female reproductive auxiliary tissues show no major sex-specific evolutionary dynamics.Genes with sex-biased expression substantially contribute to differentially expressed genes across evolutionary transitions,though without predominant directional bias toward either sex.Notably,these differentially expressed genes display significant over-representation on autosomes.A general functional divergence process is found between male and female transcriptomes across organs mainly driven by sex-specific differentially expressed genes.Collectively,our findings establish a new evolutionary framework for sex-specific expression divergence and provide novel insights into the role of reproductive constraints in shaping transcriptome evolution in mammals. 展开更多
关键词 house mouse complex population transcriptomics sex-biased gene expression sex-specific gene expression evolution transcriptome divergence
Parental exposure to ocean acidification impacts the larval development and transcriptome of the Pacific oyster Crassostrea gigas 认领 引用
13
作者 Hailun FU Wen TENG +3 位作者 Shoudu ZHANG Zhangjie PENG Jiulin CHAN Linlin ZHANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2026年第1期433-444,共12页
Atmospheric carbon dioxide(CO2)levels are escalating at an unprecedented rate,leading to the phenomenon of ocean acidification(OA).Parental exposure to acidification has the potential to enhance offspring resilienc... Atmospheric carbon dioxide(CO2)levels are escalating at an unprecedented rate,leading to the phenomenon of ocean acidification(OA).Parental exposure to acidification has the potential to enhance offspring resilience through cross-generation plasticity.In this study,we analyzed larval growth and transcriptomic profiles in the Pacific oyster,Crassostrea gigas,a species of significant ecological relevance,under both control and elevated CO2conditions experienced by their parental generation.Our findings indicate that the oyster populations exposed to OA exhibited a higher incidence of abnormalities during the D-shaped larval stage,followed by accelerated growth at the eyed stage.Through a comparative transcriptomic investigation of eyed larvae(25 d after fertilization),we observed that parental exposure to OA substantially influenced the gene expression in the offspring.Genes associated with lipid catabolism and shell formation were notably upregulated in oysters with parental OA exposure,potentially playing a role in cross-generational conditioning and conferring resilience to OA stressors.These results underscore the profound impact of OA on oyster larval development via cross-generational mechanisms and shed light on the molecular underpinnings of cross-generation plasticity. 展开更多
关键词 cross-generation plasticity oyster ocean acidification(OA) larval development transcriptome
暂未订购 下载PDF
Full-Length Transcriptome Analysis of the Pituitary of Pre-Matured and Artificially Matured Female Japanese Eel(Anguilla japonica) 认领 引用
14
作者 GAO Yu LI Jiangling +10 位作者 FENG Zhenguang HUANG Mingxi LI Wen XU Ruihan CHEN Ruoxue BAN Yu LIANG Jingjie HUANG Yan XIE Yangjie CHEN Jianchun CHEN Tiansheng 《Journal of Ocean University of China》 SCIE CAS CSCD 2026年第3期1041-1054,共14页
The Japanese eel(Anguilla japonica)is a catadromous migratory fish with a high economic value.The gonads of captive eels do not mature naturally.Under artificial maturation,the hypothalamic-pituitary-gonadal(HPG)axis ... The Japanese eel(Anguilla japonica)is a catadromous migratory fish with a high economic value.The gonads of captive eels do not mature naturally.Under artificial maturation,the hypothalamic-pituitary-gonadal(HPG)axis is activated,and the maturation coefficient of the gonads increased significantly.Prior research investigations into Japanese eel breeding have primarily focused on elucidating the mechanisms underlying gonadal development.However,the problems plaguing the artificial breeding of Japanese eels have remained unsolved.Additionally,the insights into the molecular mechanisms within the pituitary,which acts as an upstream regulatory hub,and its impact on ovarian development are limited.To address these,the pre-matured pituitary(PP)and artificially matured pituitary(AP)tissues from female Japanese eels were subjected to transcriptomics using a method combining PacBio Iso-seq with Illumina RNA-Seq.PacBio sequencing produced 19576 filtered consensus sequences from PP and 39828 from AP.Then,we predicted gene regulatory elements such as alternative polyadenylation,transcription factors,and long noncoding RNAs.A total of 3016 differentially expressed genes(DEGs)were identified based on the Illumina RNA-seq data.Further gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed.Several DEGs that were upregulated in the pituitary were identified.These genes,including nr5a2,pgr,greb1,smad4,bmp5,ar,lhb,adcy5,and tgfbr3,most likely regulate downstream gonadal development via the pituitary.In conclusion,this study obtained the differential expression and full-length transcriptomes of the pituitary in the pre-matured and artificially matured female Japanese eel.These results offer substantial evidence for developing more efficient sex hormones during the captive breeding of eels,while also furnishing a scientific basis for the conservation of eel resources. 展开更多
关键词 Anguilla japonica pituitary transcriptome analysis gonad maturation
暂未订购 下载PDF
Transcriptome analysis of genes and pathways associated with growth performance in eyestalks of Exopalaemon carinicauda under different light intensities 认领 引用
15
作者 Guifang JIA Chengsong ZHANG Fuhua LI 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2026年第3期1253-1266,共14页
Light is one of the important environmental factors on life activities of aquatic animals.Eyestalks are a multifunctional organ of crustaceans that conducts light signals,produces neurohormones,and regulates growth.Ho... Light is one of the important environmental factors on life activities of aquatic animals.Eyestalks are a multifunctional organ of crustaceans that conducts light signals,produces neurohormones,and regulates growth.However,the mechanism of growth regulation by light intensity remains poorly studied.We evaluated the growth performance of ridgetail white prawn(Exopalaemon carinicauda)under different light intensities(0,100,200,and 400 lx),and determined the regulatory mechanism of growth in response to light intensity through transcriptome analysis of eyestalks.Results show that light intensity at 100 lx significantly improved growth performance in growth rate,specific growth rate,and biomass increase rate,while 0-lx and 400-lx conditions inhibited the growth.A total of 931 DEGs were identified between the 100-lx and 0-lx groups,of which 411 were upregulated and 520 were downregulated.In addition,456 DEGs were identified between groups of 100-lx and 400-lx,including 155 upregulated DEGs and 301 downregulated DEGs.The light intensity at 100 lx significantly improved the growth performance by upregulating the oxidative phosphorylation,Toll and Imd signaling pathway,and ribosome pathway,and downregulating the tyrosine metabolism,phagosome,lysosome,autophagy,and Hippo signaling pathway,whereas 0 lx and 400 lx inhibited growth in the opposite patterns.In total,eight growth-regulation-related candidate genes,i.e.,hemocyanin,NADH dehydrogenase,cytochrome c oxidase,ATP synthase,cathepsin L,phenoloxidase activating factor,CLIP domain-containing serine protease,and 40S/60S ribosomal protein were identified.The optimal light intensity for improved growth of E.carinicauda was about 100 lx when reared indoor.These data shall provide key information for further understanding of the regulatory mechanism of light intensity on the growth performance of this species. 展开更多
关键词 Exopalaemon carinicauda eyestalk growth performance light intensity transcriptome
暂未订购 下载PDF
Impact of machine learning-driven analysis of blood transcriptomes in multiple sclerosis 认领 引用
16
作者 Alessandro Digilio Cinthia Farina 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第8期3557-3558,共2页
Multiple sclerosis(MS)is a chronic disorder of the central nervous system characterized by multifocal lesions where inflammation,demyelination,and neurodegeneration occur(Jakimovski et al.,2024).MS diagnosis primarily... Multiple sclerosis(MS)is a chronic disorder of the central nervous system characterized by multifocal lesions where inflammation,demyelination,and neurodegeneration occur(Jakimovski et al.,2024).MS diagnosis primarily relies on the demonstration of dissemination in time and space of the lesions based on clinical,magnetic resonance imaging(MRI),and cerebrospinal fluid assessments(Jakimovski et al.,2024). 展开更多
关键词 multifocal lesions inflammation cerebrospinal fluid assessments jakimovski blood transcriptomes diagnosis multiple sclerosis ms multiple sclerosis resonance imaging mri
暂未订购 下载PDF
Integrated hepatic transcriptome and metabolome reveal the mechanisms of Jiangtang Tiaozhi formula on improving glycolipid metabolic disorder 认领 引用 被引量：1
17
作者 Jia-Xing Tian Yan-Jiao Zhang +7 位作者 Yu-Xin Zhang Jia-Hua Wei Xin-Yi Fang Run-Yu Miao Kai-Le Ma Hui-Fang Guan Xin-Miao Wang Hao-Ran Wu 《World Journal of Diabetes》 SCIE 2026年第2期127-143,共17页
BACKGROUND Glycolipid metabolic disorder includes a series of chronic diseases that are closely associated with disturbances in both glucose and lipid metabolism.Jiangtang Tiaozhi formula(JTTZF)demonstrating significa... BACKGROUND Glycolipid metabolic disorder includes a series of chronic diseases that are closely associated with disturbances in both glucose and lipid metabolism.Jiangtang Tiaozhi formula(JTTZF)demonstrating significant hypoglycemic,lipidmodifying,and anti-inflammatory effects.However,the specific molecular mechanisms underlying JTTZF’s hepatoprotective effects and its ability to ameliorate glycolipid metabolic disorder remain largely unexplored.AIM To investigate how JTTZF improves glycolipid metabolic disorder using hepatic transcriptome and metabolome analyses.METHODS To induce glycolipid metabolic disorder,male C57BL/6J mice were fed a high-fat diet(HFD)for 12 weeks,after which they received an 8-week administration of JTTZF.Liver tissues were analyzed using transcriptomics and metabolomics.Real-time quantitative polymerase chain reaction validated key gene expression.RESULTS Metabolomics data revealed that JTTZF significantly regulated HFD-induced alterations in glycolipid metabolism,with notable changes in pathways such as the pentose phosphate pathway,steroid hormone biosynthesis,and purine metabolism.Transcriptomics profiles indicated that JTTZF exerted regulatory effects on lipid and glucose metabolism,primarily through pathways including peroxisome proliferators-activated receptor signaling,drug metabolism other enzymes,and regulation of lipolysis in adipocytes.Real-time quantitative polymerase chain reaction confirmed that JTTZF modulated pivotal genes associated with fatty acid synthesis,lipolysis,insulin resistance,energy metabolism,and inflammation.These findings suggest that JTTZF may act through multiple pathways to improve glycolipid metabolic disorder.CONCLUSION JTTZF can ameliorate the glycolipid metabolic disorder induced by HFD-diet by regulating lipid metabolism and improving insulin tolerance. 展开更多
关键词 Glycolipid metabolic disorder Hepatic transcriptome Hepatic metabolome Jiangtang Tiaozhi formula Multiomics
暂未订购 下载PDF
Transcriptome profiling of ocular surface ectoderm derived from embryonic stem cells,and identification of CACNG6 and AQP3 as its surface markers 认领 引用
18
作者 Lu Sun Yu-Ming Li +8 位作者 Yu-Wen Song Yi-Chen Yang Lian Duan Yang Gao Jian-Xin Li Yan-Kun Yu Kun-Peng Pang Guang-Fu Dang Can-Wei Zhang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2026年第8期1440-1450,共11页
●AIM:To investigate the transcriptional profiling of ocular surface ectoderm(OSE)derived from human embryonic stem cells(hESC),and identified CACNG6 and AQP3 as the surface markers of OSE.●METHODS:hESCs were differe... ●AIM:To investigate the transcriptional profiling of ocular surface ectoderm(OSE)derived from human embryonic stem cells(hESC),and identified CACNG6 and AQP3 as the surface markers of OSE.●METHODS:hESCs were differentiated into OSE,neuroectoderm(NE),surface ectoderm(SE),and other surface ectoderm(OE)cells in vitro.RNA-seq was performed to analyze transcriptomic profiling of hESC-derived OSE,NE,OE,and SE.The differential expressed genes(DEGs)were identified,and Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)databases,and protein-protein interaction(PPI)network analyses were performed to screen the signals and hub genes associated to OSE commitment.Also,the highly expressed transcription factors(TFs)and membrane proteins(MPs)in OSE cells were identified.●RESULTS:Transcriptome analysis revealed that OSE development is dually regulated by signals associated with both SE and NE development.The signaling pathways such as Hippo,encoding extracellular matrix(ECM)-receptor interaction,and transforming growth factor-β(TGF-β)might delineate the surface ectodermal phenotype of OSE,with FN1,COL1A1,and TGFB1 identified as hub genes.Additionally,pathways such as axon guidance,might elucidate the influence of NE in OSE commitment,and with PAX6,LHX2,FOXG1,SOX2,MSI1,and DCLK1 recognized as the hub genes.Genes implicated in retinoic acid(RA)synthesis(ALDH1A1,ALDH1A3,and RDH10)exhibited high expression in OSE,indicating the significant role of the RA signaling pathway in OSE development.Furthermore,OSEspecific transcription factors and surface markers(CACNG6 and AQP3)were identified.●CONCLUSION:This study reveals the transcriptome profiling of OSE,which could provide insights into the characteristics of OSE and the underlying molecular mechanisms involved in its derivation. 展开更多
关键词 ocular surface ectoderm transcriptome human embryonic stem cells corneal epithelial development surface markers
暂未订购 下载PDF
Letter to the Editor:Hepatic transcriptome and metabolome analysis of Jiangtang Tiaozhi formula 认领 引用
19
作者 Shu-Meng Huang Di-Guang Wen 《World Journal of Diabetes》 SCIE 2026年第6期216-218,共3页
Jiangtang Tiaozhi formula(JTTZF)has been widely used in the management of glycolipid metabolic disorders,yet its underlying molecular mechanisms remain incompletely understood.Tian et al recently published a study in ... Jiangtang Tiaozhi formula(JTTZF)has been widely used in the management of glycolipid metabolic disorders,yet its underlying molecular mechanisms remain incompletely understood.Tian et al recently published a study in the World Journal of Diabetes,which reported a comprehensive hepatic transcriptomic and metabolomic analysis that provides important insights into the regulatory effects of JTTZF on glucose and lipid homeostasis.This multi-omics strategy offers a valuable systems-level perspective on the pharmacological actions of this traditional Chinese medicine formulation.In this commentary,we highlight the strengths of the study and discuss several aspects that warrant further investigation to enhance mechanistic depth and translational relevance.These include dose-response relationships,cellular heterogeneity,causal validation of key pathways,identification of active constituents,and long-term safety evaluation.We also emphasize the potential role of host-related factors,such as immune regulation and gut microbiota-associated metabolic interactions,in influencing therapeutic responsiveness. 展开更多
关键词 Glycolipid metabolic disorder Jiangtang Tiaozhi formula Hepatic transcriptome Hepatic metabolome Multi-omics analysis Insulin resistance Lipid metabolism Glucose metabolism
暂未订购 下载PDF
A genome-wide association study integrated with transcriptome analysis to identify boron efficiency-related candidate genes and favorable haplotypes in Brassica napus L. 认领 引用
20
作者 Ziwei Zhang Haoqiang Zhai +2 位作者 Yingpeng Hua Sheliang Wang Fangsen Xu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第7期2723-2738,共16页
Rapeseed(Brassica napus L.)is a major oil crop worldwide that is vigorously promoted for cultivation in China.Boron(B)is an essential micronutrient for plant growth and development.However,the agricultural soils in ra... Rapeseed(Brassica napus L.)is a major oil crop worldwide that is vigorously promoted for cultivation in China.Boron(B)is an essential micronutrient for plant growth and development.However,the agricultural soils in rapeseed planting areas often show either B deficiency or severe B deficiency.Increasing the resistance to B deficiency is a pivotal goal in the breeding of rapeseed,yet the genetic basis for variations in B efficiency-related traits remains unclear.In this study,a natural population with 391 rapeseed accessions and a nutrient solution system were used to investigate B efficiency-related traits,including relative root length(RRL),shoot dry weight(SDW),root dry weight(RDW),and B efficiency coefficient(BEC),all of which exhibited extensive phenotypic variations under B deficiency.Through a genome-wide association study(GWAS)of B efficiency-related traits using high-density SNP markers obtained from whole-genome resequencing,106 significantly associated SNPs were identified by employing both the general linear model and the mixed linear model.Among these SNP loci,two prominent SNP clusters were detected on chrA03:14,087,835-14,764,672 and chrC03:20,110,319-22,135,492at low B levels across three repeated experiments of multiple traits.Integrating those results with a transcriptome analysis,four genes exhibiting higher differentially expressed fold-change along with favorable haplotypes within the promoter or coding region,BnaA03g29020D,BnaA03g29440D,BnaC03g33010D,and BnaC03g34490D,were identified as candidate genes that could potentially be involved in efficient B utilization,and their favorable haplotypes were found to improve seedling growth and productivity under B deficiency.Considering the lack of B mineral resources in China,the rapid and accurate identification of more B-efficient alleles and studying the genetic mechanism underlying crop responses to B deficiency have important theoretical and practical significance for cultivating B-efficient varieties and maintaining green,sustainable agriculture. 展开更多
关键词 rapeseed boron deficiency GWAS transcriptomic analysis favorable haplotypes B efficiency
暂未订购 下载PDF
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
上一页 1 2 … 100 下一页 到第 页
在线咨询 使用帮助 返回顶部 意见反馈