期刊文献+
任意字段
题名或关键词
题名
关键词
摘要
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到303篇文章
< 1 2 … 16 >
每页显示 20 50 100
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
显示方式：
Long noncoding RNA X-inactive specific transcript regulates NLR family pyrin domain containing 3/caspase-1-mediated pyroptosis in diabetic nephropathy 认领 引用 被引量：11
1
作者 Jia Xu Qin Wang +4 位作者 Yi-Fan Song Xiao-Hui Xu He Zhu Pei-Dan Chen Ye-Ping Ren 《World Journal of Diabetes》 SCIE 2022年第4期358-375,共18页
BACKGROUND NLRP3-mediated pyroptosis is recognized as an essential modulator of renal disease pathology.Long noncoding RNAs(lncRNAs)are active participators of diabetic nephropathy(DN).X inactive specific transcript(X... BACKGROUND NLRP3-mediated pyroptosis is recognized as an essential modulator of renal disease pathology.Long noncoding RNAs(lncRNAs)are active participators of diabetic nephropathy(DN).X inactive specific transcript(XIST)expression has been reported to be elevated in the serum of DN patients.AIM To evaluate the mechanism of lncRNA XIST in renal tubular epithelial cell(RTEC)pyroptosis in DN.METHODS A DN rat model was established through streptozotocin injection,and XIST was knocked down by tail vein injection of the lentivirus LV sh-XIST.Renal metabolic and biochemical indices were detected,and pathological changes in the renal tissue were assessed.The expression of indicators related to inflammation and pyroptosis was also detected.High glucose(HG)was used to treat HK2 cells,and cell viability and lactate dehydrogenase(LDH)activity were detected after silencing XIST.The subcellular localization and downstream mechanism of XIST were investigated.Finally,a rescue experiment was carried out to verify that XIST regulates NLR family pyrin domain containing 3(NLRP3)/caspase-1-mediated RTEC pyroptosis through the microRNA-15-5p(miR-15b-5p)/Toll-like receptor 4(TLR4)axis.RESULTS XIST was highly expressed in the DN models.XIST silencing improved renal metabolism and biochemical indices and mitigated renal injury.The expression of inflammation and pyroptosis indicators was significantly increased in DN rats and HG-treated HK2 cells;cell viability was decreased and LDH activity was increased after HGtreatment. Silencing XIST inhibited RTEC pyroptosis by inhibiting NLRP3/caspase-1. Mechanistically,XIST sponged miR-15b-5p to regulate TLR4. Silencing XIST inhibited TLR4 by promotingmiR-15b-5p. miR-15b-5p inhibition or TLR4 overexpression averted the inhibitory effect ofsilencing XIST on HG-induced RTEC pyroptosis.CONCLUSIONSilencing XIST inhibits TLR4 by upregulating miR-15b-5p and ultimately inhibits renal injury inDN by inhibiting NLRP3/caspase-1-mediated RTEC pyroptosis. 展开更多
关键词 Diabetic nephropathy Pyroptosis Renal tubular epithelial cell Long noncoding RNA X-inactive specific transcript microRNA-15b-5p Toll-like receptor 4 NLR family pyrin domain containing 3/caspase-1 pathway
暂未订购 下载PDF
Pivotal role of long non-coding ribonucleic acid-X-inactive specific transcript in regulating immune checkpoint programmed death ligand 1 through a shared pathway between miR-194-5p and miR-155-5p in hepatocellular carcinoma 认领 引用 被引量：10
2
作者 Sara M Atwa Heba Handoussa +2 位作者 Karim M Hosny Margarete Odenthal Hend M El Tayebi 《World Journal of Hepatology》 2020年第12期1211-1227,共17页
BACKGROUND Anti-programmed death therapy has thrust immunotherapy into the spotlight.However,such therapy has a modest response in hepatocellular carcinoma(HCC).Epigenetic immunomodulation is a suggestive combinatoria... BACKGROUND Anti-programmed death therapy has thrust immunotherapy into the spotlight.However,such therapy has a modest response in hepatocellular carcinoma(HCC).Epigenetic immunomodulation is a suggestive combinatorial therapy with immune checkpoint blockade.Non-coding ribonucleic acid(ncRNA)driven regulation is a major mechanism of epigenetic modulation.Given the wide range of ncRNAs that co-opt in programmed cell-death protein 1(PD-1)/programmed death ligand 1(PD-L1)regulation,and based on the literature,we hypothesized that miR-155-5p,miR-194-5p and long non-coding RNAs(lncRNAs)X-inactive specific transcript(XIST)and MALAT-1 are involved in a regulatory upstream pathway for PD-1/PD-L1.Recently,nutraceutical therapeutics in cancers have received increasing attention.Thus,it is interesting to study the impact of oleuropein on the respective study key players.AIM To explore potential upstream regulatory ncRNAs for the immune checkpoint PD-1/PD-L1.METHODS Bioinformatics tools including microrna.org and lnCeDB software were adopted to detect targeting of miR-155-5p,miR-194-5p and lncRNAs XIST and MALAT-1 to PD-L1 mRNA,respectively.In addition,Diana tool was used to predict targeting of both aforementioned miRNAs to lncRNAs XIST and MALAT-1.HCC and normal tissue samples were collected for scanning of PD-L1,XIST and MALAT-1 expression.To study the interaction among miR-155-5p,miR-194-5p,lncRNAs XIST and MALAT-1,as well as PD-L1 mRNA,a series of transfections of the Huh-7 cell line was carried out.RESULTS Bioinformatics software predicted that miR-155-5p and miR-194-5p can target PDL1,MALAT-1 and XIST.MALAT-1 and XIST were predicted to target PD-L1 mRNA.PD-L1 and XIST were significantly upregulated in 23 HCC biopsies compared to healthy controls;however,MALAT-1 was barely detected.MiR-194 induced expression elevated the expression of PD-L1,XIST and MALAT-1.However,overexpression of miR-155-5p induced the upregulation of PD-L1 and XIST,while it had a negative impact on MALAT-1 expression.Knockdown of XIST did have an impact on PD-L1 expression;however,following knockdown of the negative regulator of X-inactive specific transcript(TSIX),PD-L1 expression was elevated,and abolished MALAT-1 activity.Upon co-transfection of miR-194-5p with siMALAT-1,PD-L1 expression was elevated.Co-transfection of miR-194-5p with siXIST did not have an impact on PD-L1 expression.Upon co-transfection of miR-194 with siTSIX,PD-L1 expression was upregulated.Interestingly,the same PD-L1 expression pattern was observed following miR-155-5p cotransfections.Oleuropein treatment of Huh-7 cells reduced the expression profile of PD-L1,XIST,and miR-155-5p,upregulated the expression of miR-194-5p and had no significant impact on the MALAT-1 expression profile.CONCLUSION This study reported a novel finding revealing that opposing acting miRNAs in HCC,have the same impact on PD-1/PD-L1 immune checkpoint by sharing a common signaling pathway. 展开更多
关键词 Hepatocellular carcinoma X-inactive specific transcript MiR-155-5p MiR-194-5p Programmed cell-death protein 1/Programmed death ligand 1 Immune checkpoint
暂未订购 下载PDF
SPOROCYTELESS Is a Novel Embryophyte-Specific Transcription Repressor that Interacts with TPL and TCP Proteins in Arabidopsis 认领 引用 被引量：6
3
作者 Guang-Hui Chen Jia-Ying Sun +2 位作者 Man Liu Jie Liu Wei-Cai Yang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2014年第12期617-625,共9页
Germlines in plants are formed de novo during post-embryonic development, while little is known about the mechanism that controls this process. In Arabidopsis, the earliest gene controlling this process is SPOROCYTELE... Germlines in plants are formed de novo during post-embryonic development, while little is known about the mechanism that controls this process. In Arabidopsis, the earliest gene controlling this process is SPOROCYTELESS （SPL）. A decade ago, we showed that loss of SPL function abolished sporogenesis in both male and female organs of Arabidopsis. However, its function is unclear up to now. In this study, we showed that SPL belongs to a novel transcription repressor family specific in embryophyte, which consists of 173 members in the land plants so far. All of them contain a conserved SPL-motif in their N-terminal and an ethylene-responsive element binding factor-associated amphiphilic repression （EAR） motif in the C-terminal, therefore designated as SPL-like, EAR-containing proteins （SPEARs）. Consis- tently, SPL acts as a transcriptional repressor in yeast and tobacco cells, and SPEAR proteins are able to form homodimer and/or het- erodimer with each other in vitro. Furthermore, SPEARs interact with the TOPLESS （TPL） co-repressors via the EAR motif and TCP family transcription factors in yeast cells. Together, we propose that SPL and SPEARs most likely belong to a novel transcription repressor family in land plants which may play a variety of developmental roles in plants. 展开更多
关键词 Germline specification Sporogenesis SPL Transcription repressor Arabidopsis
暂未订购 下载PDF
The basic helix-loop-helix(bHLH)gene family in Clinacanthus nutans:insights into tissue-specific expression,stress responses,and methyl jasmonate(MeJA)responsiveness 认领 引用
4
作者 Chang An Denghang Wu +6 位作者 Ningwei Li Yixin Yao Lin Lu Yan Cheng Zongshen Zhang Ping Zheng Yuan Qin 《Medicinal Plant Biology》 2025年第1期96-109,共14页
Clinacanthus nutans is valued for its medicinal properties,largely attributed to bioactive compounds such as flavonoids,polyphenols,and other secondary metabolites.These compounds accumulate differentially across tiss... Clinacanthus nutans is valued for its medicinal properties,largely attributed to bioactive compounds such as flavonoids,polyphenols,and other secondary metabolites.These compounds accumulate differentially across tissues and are influenced by environmental cues and phytohormones like methyl jasmonate(MeJA).Transcription factors play pivotal roles in mediating these responses,with the basic helix-loop-helix(bHLH)gene family emerging as key regulators of hormone-and stress-responsive gene expression involved in secondary metabolism.In this study,182 bHLHs were identified in C.nutans,labeled CnbHLH001 to CnbHLH182 according to their chromosomal locations.Phylogenetic analysis grouped them into 26 subfamilies,with 10 classified as orphans.Structural characterization revealed subfamily-specific motif architectures and exon–intron structures.Gene duplication analysis indicated that segmental duplication was a major driver of CnbHLH family expansion,and expression analysis suggested both conserved and divergent expression among duplicated gene pairs.Tandemly duplicated genes such as CnbHLH044 to CnbHLH047 showed similar expression patterns.Synteny analysis showed that CnbHLHs in Acanthaceae species are more similar to each other than to genes in other species,suggesting a possible Acanthaceae-specific expansion.Furthermore,this study provides the first genome-wide analysis of CnbHLHs expression in response to abiotic stresses.Many CnbHLHs showed tissuespecific expression and responded differentially to MeJA and stress treatments.These findings provide a foundation for future research to explore the regulatory mechanisms of CnbHLHs and their potential to improve the medicinal properties of C.nutans. 展开更多
关键词 environmental cues stress responses secondary metabolitesthese tissue specific expression clinacanthus nutans bioactive compounds methyl jasmonate meja transcription factors basic helix loop helix genes
暂未订购 下载PDF
Transcription factors specificity protein and nuclear receptor 4A1 in pancreatic cancer 认领 引用 被引量：2
5
作者 Stephen Safe Rupesh Shrestha +3 位作者 Kumaravel Mohankumar Marcell Howard Erik Hedrick Maen Abdelrahim 《World Journal of Gastroenterology》 SCIE CAS 2021年第38期6387-6398,共12页
Specificity protein(Sp)transcription factors(TFs)Sp1,Sp3 and Sp4,and the orphan nuclear receptor 4A1(NR4A1)are highly expressed in pancreatic tumors and Sp1 is a negative prognostic factor for pancreatic cancer patien... Specificity protein(Sp)transcription factors(TFs)Sp1,Sp3 and Sp4,and the orphan nuclear receptor 4A1(NR4A1)are highly expressed in pancreatic tumors and Sp1 is a negative prognostic factor for pancreatic cancer patient survival.Results of knockdown and overexpression of Sp1,Sp3 and Sp4 in pancreatic and other cancer lines show that these TFs are individually pro-oncogenic factors and loss of one Sp TF is not compensated by other members.NR4A1 is also a prooncogenic factor and both NR4A1 and Sp TFs exhibit similar functions in pancreatic cancer cells and regulate cell growth,survival,migration and invasion.There is also evidence that Sp TFs and NR4A1 regulate some of the same genes including survivin,epidermal growth factor receptor,PAX3-FOXO1,α5-andα6-integrins,β1-,β3-andβ4-integrins;this is due to NR4A1 acting as a cofactor and mediating NR4A1/Sp1/4-regulated gene expression through GC-rich gene promoter sites.Several studies show that drugs targeting Sp downregulation or NR4A1 antagonists are highly effective inhibitors of Sp/NR4A1-regulated pathways and genes in pancreatic and other cancer cells,and the triterpenoid celastrol is a novel dual-acting agent that targets both Sp TFs and NR4A1. 展开更多
关键词 Specificity protein Nuclear receptor 4A1 Pancreatic cancer Transcription factors Ligand inhibitors Nuclear receptor 4A antagonists
暂未订购 下载PDF
Identification of epididymis-specific transcripts in the mouse and rat by transcriptional profiling 认领 引用 被引量：9
6
作者 Daniel S. Johnston Terry T. Turner +3 位作者 Joshua N. Finger Tracy L. Owtscharuk Gregory S. Kopf Scott A. Jelinsky 《Asian Journal of Andrology》 SCIE CAS 2007年第4期522-527,共6页
As part of our efforts to identify novel contraceptive targets in the epididymis we performed transcriptional profiling on each of the 10 and 19 segments of the mouse and rat epididymidis, respectively, using Affymetr... As part of our efforts to identify novel contraceptive targets in the epididymis we performed transcriptional profiling on each of the 10 and 19 segments of the mouse and rat epididymidis, respectively, using Affymetrix whole genome microarrays. A total of 17 096 and 16 360 probe sets representing transcripts were identified as being expressed in the segmented mouse and rat epididymal transcriptomes, respectively. Comparison of the expressed murine transcripts against a mouse transcriptional profiling database derived from 22 other mouse tissues identified 77 transcripts that were expressed uniquely in the epididymis. The expression of these genes was further evaluated by reverse transcription polymerase chain reaction （RT-PCR） analysis of RNA from 21 mouse tissues. RT-PCR analysis confirmed epididymis-specific expression of Defensin Beta 13 and identified two additional genes with expression restricted only to the epididymis and testis. Comparison of the 16 360 expressed transcripts in the rat epididymis with data of 21 other tissues from a rat transcriptional profiling database identified 110 transcripts specific for the epididymis. Sixty-two of these transcripts were further investigated by qPCR analysis. Only Defensin 22 （E3 epididymal protein） was shown to be completely specific for the epididymis. In addition, 14 transcripts showed more than 100-fold selective expression in the epididymis. The products of these genes might play important roles in epididymal and/or sperm function and further investigation and validation as contraceptive targets are warranted. The results of the studies described in this report are available at the Mammalian Reproductive Genetics （MRG） Database （http：//mrg. genetics.washington.edu/）. （Asian J Androl 2007July; 9： 522-527） 展开更多
关键词 mouse rat epididymis transcriptional profiling contraception microarray epididymis-specific epididymis-selective
暂未订购 下载PDF
An in silicoAnalysis of Upstream Regulatory Modules (URMs) of Tapetum Specific Genes to Identify Regulatory cis-Elements and Transcription Factors 认领 引用 被引量：1
7
作者 Preeti Apurve Sharma Pradeep Kumar Burma 《American Journal of Molecular Biology》 CAS 2018年第1期13-25,共13页
The present work presents an iin silicoi analysis of Upstream Regulatory Modules (URMs) of genes expressed in tapetum specific manner in dicotyledon and monocotyledon plants. In the current analysis, we identified sev... The present work presents an iin silicoi analysis of Upstream Regulatory Modules (URMs) of genes expressed in tapetum specific manner in dicotyledon and monocotyledon plants. In the current analysis, we identified several motifs conserved in these URMs of which ten were observed to be part of known icisi-elements using tools and databases like MEME, PLACE, MAST and TFSEARCH. We also identified that binding sites for two transcription factors, DOF and WRKY71 were found to be present in majority of the URMs. 展开更多
关键词 Tapetum Specific Promoter cis-Elements Transcription Factors
暂未订购 下载PDF
PpBBX32 and PpZAT5 modulate temperature-dependent and tissue-specific anthocyanin accumulation in peach fruit 认领 引用 被引量：2
8
作者 Dan Huang Lei Xue +6 位作者 Yueqin Lu Mengfei Liu Kui Lin-Wang Andrew C.Allan Bo Zhang Kunsong Chen Changjie Xu 《Horticulture Research》 SCIE CSCD 2024年第10期31-47,共17页
Anthocyanins are important compounds for fruit quality and nutrition.The R2R3 MYB transcription factor PpMYB10.1 is known to be critical for regulating anthocyanin accumulation in peach.However,regulatory factors upst... Anthocyanins are important compounds for fruit quality and nutrition.The R2R3 MYB transcription factor PpMYB10.1 is known to be critical for regulating anthocyanin accumulation in peach.However,regulatory factors upstream of PpMYB10.1 which control temperature-dependent,cultivar-contrasted and tissue-specific anthocyanin accumulation remain to be determined.In this study,differential anthocyanin accumulation in the outer flesh near the peel(OF)of peach[Prunus persica(L.)Batsch]was observed between cultivars‘Zhonghuashoutao’and‘Dongxuemi’,as well as among different storage temperatures and different fruit tissues of‘Zhonghuashoutao’.By cross-comparisons of RNA-Seq data of samples with differential anthocyanin accumulation,transcription factor genes PpBBX32 and PpZAT5 were identified.These were functionally characterized as two positive regulators for anthocyanin accumulation via transient expression and genetic transformation.Various interaction assays revealed that both PpBBX32 and PpZAT5 can directly activate the PpMYB10.1 promoter and meanwhile interact at protein level as a PpZAT5-PpBBX32-PpMYB10.1 complex.Furthermore,the results of in silico analysis and exogenous application of methyl jasmonate(MeJA)indicated that MeJA favored anthocyanin accumulation,while it was also found that anthocyanin accumulation as well as PpBBX32 and PpZAT5 expression correlated significantly with endogenous JA and JA-Ile in different fruit tissues.In summary,PpBBX32 and PpZAT5 are upstream activators of PpMYB10.1,allowing JAs to take part in temperature-dependent and tissue-specific anthocyanin accumulation by modulating their expression.This work enriches the knowledge of the transcriptional regulatory mechanisms for differential anthocyanin accumulation under internal and external factors. 展开更多
关键词 temperature dependent peach fruit tissue specific ppbbx ppmyb anthocyanin accumulation r r myb transcription factor outer flesh
暂未订购 下载PDF
lncRNA XIST靶向miR-21-5p对脂多糖诱导的心肌细胞凋亡、炎症反应的影响 认领 引用
9
作者 周晓铎 张松雨 +2 位作者 李燕 王琰淏 韩克丽 《中国免疫学杂志》 CAS CSCD 北大核心 2026年第4期782-787,共6页
目的:探究长链非编码RNA(lncRNA)X染色体失活特异转录本(XIST)对脂多糖(LPS)诱导的心肌细胞凋亡、炎症反应的影响及潜在机制。方法:体外培养大鼠心肌H9c2细胞,分为对照组、LPS组、si-NC组、si-XIST组、si-XIST+anti-NC组、si-XIST+anti-... 目的:探究长链非编码RNA(lncRNA)X染色体失活特异转录本(XIST)对脂多糖(LPS)诱导的心肌细胞凋亡、炎症反应的影响及潜在机制。方法:体外培养大鼠心肌H9c2细胞,分为对照组、LPS组、si-NC组、si-XIST组、si-XIST+anti-NC组、si-XIST+anti-miR-21-5p组。CCK-8法测定细胞活力;流式细胞术测定细胞凋亡;ELISA检测上清炎症因子IL-1β、IL-6和TNF-α水平;RT-qPCR检测细胞lncRNA XIST、miR-21-5p、程序性细胞死亡蛋白4(PDCD4)mRNA表达;Western blot检测细胞PDCD4、BCL2相关X蛋白(Bax)、B细胞淋巴瘤-2(Bcl-2)、cleaved caspase-3、cleaved caspase-9蛋白表达。结果:与对照组相比,LPS组H9c2细胞活力、Bcl-2蛋白水平降低,凋亡率、上清IL-1β、TNF-α和IL-6水平、lncRNA XIST、PDCD4 mRNA和蛋白表达、Bax、cleaved caspase-3和cleaved caspase-9蛋白水平升高(P<0.05);与si-NC组相比,si-XIST组H9c2细胞活力、Bcl-2蛋白水平升高,凋亡率、上清IL-1β、TNF-α和IL-6水平、lncRNA XIST、PDCD4 mRNA和蛋白表达、Bax、cleaved caspase-3和cleaved caspase-9蛋白水平降低(P<0.05);下调miR-21-5p可明显减弱lncRNA XIST敲低对LPS诱导的H9c2细胞凋亡和炎症反应的抑制作用。结论:敲低lncRNA XIST可能通过上调miR-21-5p,抑制PDCD4表达,减轻LPS诱导的H9c2细胞炎症反应,并抑制细胞凋亡。 展开更多
关键词 长链非编码RNA X染色体失活特异转录本 脂多糖 心肌细胞 miR-21-5p 凋亡 炎症反应
暂未订购 下载PDF
急性呼吸窘迫综合征患儿血清ELK1、RUNX1水平与病情严重程度及预后的关系 认领 引用
10
作者 张迎旭 赵媛媛 张孝兴 《检验医学与临床》 CAS 2026年第3期296-302,共7页
目的探讨急性呼吸窘迫综合征(ARDS)患儿血清红细胞转化特异性转录因子样基因1(ELK1)、Runt相关转录因子1(RUNX1)水平与病情严重程度及预后的关系。方法选取2021年1月至2023年1月该院收治的205例ARDS患儿作为ARDS组,另选取同期在该院体检... 目的探讨急性呼吸窘迫综合征(ARDS)患儿血清红细胞转化特异性转录因子样基因1(ELK1)、Runt相关转录因子1(RUNX1)水平与病情严重程度及预后的关系。方法选取2021年1月至2023年1月该院收治的205例ARDS患儿作为ARDS组,另选取同期在该院体检的112例健康儿童作为对照组。根据病情严重程度将ARDS患儿分为轻度ARDS组(53例)、中度ARDS组(83例)、重度ARDS组(69例)。根据入院后28 d内是否发生死亡,将ARDS患儿分为预后不良组和预后良好组。采用酶联免疫吸附试验检测血清ELK1、RUNX1水平。采用Spearman相关分析ARDS患儿血清ELK1、RUNX1水平与病情严重程度的相关性。采用多因素Logistic回归分析ARDS患儿预后不良的影响因素。绘制受试者工作特征(ROC)曲线分析血清ELK1、RUNX1对ARDS患儿预后不良的预测价值。结果ARDS组血清ELK1水平高于对照组,RUNX1水平低于对照组,差异均有统计学意义(P<0.05)。重度ARDS组血清ELK1水平高于轻度ARDS组、中度ARDS组,且中度ARDS组高于轻度ARDS组,差异均有统计学意义(P<0.05)。重度ARDS组血清RUNX1水平低于轻度ARDS组、中度ARDS组,且中度ARDS组低于轻度ARDS组,差异均有统计学意义(P<0.05)。Spearman相关分析结果显示,ARDS患儿血清ELK1水平与病情严重程度呈正相关(rs=0.797,P<0.001),血清RUNX1水平与病情严重程度呈负相关(rs=-0.783,P<0.001)。预后不良组呼气末正压、ELK1水平高于预后良好组,氧合指数、清蛋白、RUNX1水平低于预后良好组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,氧合指数、清蛋白和RUNX1水平升高是ARDS患儿预后不良的保护因素(P<0.05),血清ELK1水平升高是ARDS患儿预后不良的危险因素(P<0.05)。ROC曲线分析结果显示,血清ELK1、RUNX1联合预测ARDS患儿预后不良的曲线下面积(AUC)为0.875,大于血清ELK1、RUNX1单独预测的AUC(Z=3.215、3.376,P=0.001、0.001)。结论ARDS患儿血清ELK1水平升高,RUNX1水平降低,二者水平与病情严重程度和预后有关,血清ELK1、RUNX1联合检测对ARDS患儿预后不良有较高的预测价值。 展开更多
关键词 急性呼吸窘迫综合征 红细胞转化特异性转录因子样基因1 Runt相关转录因子1 病情严重程度 预后
暂未订购 下载PDF
lncRNA XIST靶向miR-182-5p/HIF-2α分子轴对滋养层细胞生物学行为特性影响的机制研究 认领 引用
11
作者 黄燕 张林静 +2 位作者 汪洪林 张婷 李波 《中国比较医学杂志》 CAS 北大核心 2026年第6期11-20,共10页
目的探究长链非编码RNA(lncRNA)X染色体失活特异性转录本(XIST)调控滋养层细胞生物学活性的分子机制。方法体外培养人绒毛膜滋养层细胞HTR-8/Svneo,分为对照组、干扰空载组(转染siNC)和si-XIST-1组(转染si-XIST-1)、si-XIST-1+anti-NC组... 目的探究长链非编码RNA(lncRNA)X染色体失活特异性转录本(XIST)调控滋养层细胞生物学活性的分子机制。方法体外培养人绒毛膜滋养层细胞HTR-8/Svneo,分为对照组、干扰空载组(转染siNC)和si-XIST-1组(转染si-XIST-1)、si-XIST-1+anti-NC组(共转染si-XIST-1和anti-NC)、si-XIST-1+anti-182-5p组(共转染si-XIST-1和anti-182-5p)。采用RT-qPCR检测细胞中XIST、miR-182-5p和低氧诱导因子2α(HIF-2α)mRNA表达;CCK-8实验和EdU实验检测细胞增殖活力;流式细胞术检测细胞凋亡;划痕愈合实验、Transwell实验检测细胞迁移、侵袭能力;Western blot检测细胞中HIF-2α、cleaved-caspase-3、Bcl-2、Bax、基质金属蛋白(MMP)-2、MMP-9蛋白表达;双荧光素酶报告实验验证miR-182-5p与XIST、HIF-2α的靶向关系。结果与对照组、干扰空载组比较,si-XIST-1组XIST、HIF-2αmRNA水平、凋亡率及HIF-2α、cleaved-caspase-3、Bax蛋白水平降低,miR-182-5p水平、细胞活力、EdU阳性率、划痕愈合率、侵袭细胞数及Bcl-2、MMP-2、MMP-9蛋白水平升高(P<0.05);与si-XIST-1组和si-XIST-1+anti-NC组比较,si-XIST-1+anti-182-5p组HIF-2αmRNA水平、凋亡率及HIF-2α、cleaved-caspase-3、Bax蛋白水平升高,miR-182-5p水平、细胞活力、EdU阳性率、划痕愈合率、侵袭细胞数及Bcl-2、MMP-2、MMP-9蛋白水平降低(P<0.05)。lncRNA XIST可靶向HTR-8/Svneo细胞中的miR-182-5p,HIF-2α是miR-182-5p的靶标。结论敲低lncRNA XIST可能是通过竞争性结合miR-182-5p来抑制HIF-2α表达,进而增强HTR-8/Svneo细胞增殖、迁移和侵袭能力,并抑制细胞凋亡。 展开更多
关键词 子痫前期 长链非编码RNA X染色体失活特异性转录本 microRNA-182 低氧诱导因子2α 滋养层细胞
暂未订购 下载PDF
急性脑梗死患者血清ITLN-1、DUSP1、KLF14水平与溶栓前NIHSS评分及静脉溶栓后血管再闭塞的关系 认领 引用
12
作者 李海云 贾艳丽 +2 位作者 闫晓杰 郭佳丽 王天俊 《检验医学与临床》 CAS 2026年第11期1467-1473,1479,共7页
目的探讨急性脑梗死(ACI)患者血清凝集素-1(ITLN-1)、双重特异性磷酸酶1(DUSP1)、Krüpple样转录因子14(KLF14)水平与溶栓前美国国立卫生研究院卒中量表(NIHSS)评分及静脉溶栓后血管再闭塞的关系。方法选取2021年6月至2025年6月张... 目的探讨急性脑梗死(ACI)患者血清凝集素-1(ITLN-1)、双重特异性磷酸酶1(DUSP1)、Krüpple样转录因子14(KLF14)水平与溶栓前美国国立卫生研究院卒中量表(NIHSS)评分及静脉溶栓后血管再闭塞的关系。方法选取2021年6月至2025年6月张家口市第一医院收治的172例ACI患者作为研究对象,根据溶栓后是否发生血管再闭塞分为未再闭塞组和再闭塞组。检测所有研究对象血清ITLN-1、DUSP1、KLF14水平,收集所有研究对象基线资料。采用Pearson相关分析ACI发生血管再闭塞患者血清ITLN-1、DUSP1、KLF14水平与溶栓前NIHSS评分的相关性;采用多因素Logistic回归分析ACI患者发生血管再闭塞的影响因素;绘制受试者工作特征(ROC)曲线分析血清ITLN-1、DUSP1、KLF14单独及三者联合对ACI患者发生血管再闭塞的预测价值;采用相对危险度分析血清ITLN-1、DUSP1、KLF14水平与ACI患者发生血管再闭塞的关系。结果未再闭塞组130例,再闭塞组42例。再闭塞组溶栓前NIHSS评分高于未再闭塞组,差异有统计学意义(P<0.05)。再闭塞组血清ITLN-1、DUSP1、KLF14水平均低于未再闭塞组,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,ACI发生血管再闭塞患者血清ITLN-1、DUSP1、KLF14水平与溶栓前NIHSS评分均呈负相关(r=-0.486、-0.490、-0.473,P<0.05)。多因素Logistic回归分析结果显示,血清ITLN-1、DUSP1、KLF14水平升高均为ACI患者发生血管再闭塞的保护因素(P<0.05),溶栓前NIHSS评分升高为ACI患者发生血管再闭塞的危险因素(P<0.05)。ROC曲线分析结果显示,血清ITLN-1、DUSP1、KLF14单独预测ACI患者发生血管再闭塞的曲线下面积(AUC)分别为0.860、0.868、0.865,三者联合检测预测ACI患者发生血管再闭塞的AUC为0.976,血清ITLN-1、DUSP1、KLF14联合检测的AUC大于单独预测的AUC(Z=3.962、3.790、3.906,P<0.001)。相对危险度分析结果显示,血清ITLN-1、DUSP1、KLF14高表达ACI患者发生血管再闭塞分别是血清ITLN-1、DUSP1、KLF14低表达ACI患者发生血管再闭塞的0.121、0.147、0.159倍(P<0.05)。结论ACI患者血清ITLN-1、DUSP1、KLF14水平均降低,与溶栓前NIHSS评分具有一定相关性,且ITLN-1、DUSP1、KLF14均对静脉溶栓后血管再闭塞具有一定辅助预测价值。 展开更多
关键词 急性脑梗死 凝集素-1 双重特异性磷酸酶1 Krüpple样转录因子14 美国国立卫生研究院卒中量表 血管再闭塞
暂未订购 下载PDF
lncRNA GAS6-AS1调节miR-326/E2F1轴对神经母细胞瘤细胞增殖、迁移和侵袭影响的实验研究 认领 引用
13
作者 安艳晓 祁艳卫 仲智勇 《现代检验医学杂志》 CAS 2026年第1期46-50,69,共5页
目的探究长链非编码RNA(lncRNA)生长阻滞特异性基因6反义RNA1(GAS6-AS1)通过调节微小RNA-326(miR-326)/E2F转录因子1(E2F1)轴对神经母细胞瘤(NB)细胞增殖、迁移和侵袭的影响。方法收集23例2021年8月~2023年1月在河北省儿童医院接受治疗... 目的探究长链非编码RNA(lncRNA)生长阻滞特异性基因6反义RNA1(GAS6-AS1)通过调节微小RNA-326(miR-326)/E2F转录因子1(E2F1)轴对神经母细胞瘤(NB)细胞增殖、迁移和侵袭的影响。方法收集23例2021年8月~2023年1月在河北省儿童医院接受治疗的NB患儿瘤组织及瘤旁组织,实时荧光定量聚合酶链反应(qRT-PCR)检测NB组织和细胞中lncRNA GAS6-AS1、miR-326、E2F1 mRNA表达水平并用双荧光素酶实验检测三者间的关系。将SK-N-SH细胞随机分为对照(control)组、短发夹RNA阴性对照(sh-NC)组、GAS6-AS1短发夹RNA(sh-GAS6-AS1)组、sh-GAS6-AS1+抑制剂阴性对照(anti-NC)组、sh-GAS6-AS1+miR-326抑制剂(anti-miR-326)组。qRT-PCR检测各组细胞中lncRNA GAS6-AS1和miR-326表达水平,克隆形成实验、划痕实验、Transwell实验及蛋白免疫印迹法分别检测NB细胞增殖、迁移、侵袭及E2F1、细胞周期蛋白D1(Cyclin D1)、基质金属蛋白酶(MMP)-2、MMP-9蛋白表达;构建裸鼠移植瘤模型,随机将小鼠分为sh-NC组和sh-GAS6-AS1组,qRT-PCR检测移植瘤组织中lncRNA GAS6-AS1和miR-326水平,免疫组化检测移植瘤组织中E2F1蛋白表达。结果与瘤旁组织和人正常神经细胞视网膜色素上皮细胞D-407比较,NB瘤组织和SK-N-SH细胞中lncRNA GAS6-AS1水平、E2F1 mRNA表达升高,miR-326表达降低,差异具有统计学意义(t=7.221~53.271,均P<0.05);lncRNA GAS6-AS1及E2F1与miR-326存在靶向关系。与sh-NC组比较,sh-GAS6-AS1组细胞克隆形成数目、划痕愈合率、侵袭细胞数目、lncRNA GAS6-AS1、E2F1、Cyclin D1、MMP-2、MMP-9表达下降,miR-326表达上升,差异具有统计学意义(q=8.706~16.489,均P<0.05)。与sh-GAS6-AS1+anti-NC组比较,sh-GAS6-AS1+anti-miR-326组克隆形成数目、划痕愈合率、侵袭细胞数目、E2F1、Cyclin D1、MMP-2、MMP-9表达升高,miR-326表达降低,差异具有统计学意义(q=4.173~13.407,均P<0.05)。裸鼠实验显示,与sh-NC组比较,sh-GAS6-AS1组肿瘤质量、体积均下降,lncRNA GAS6-AS1、E2F1表达下调,miR-326表达上调,差异具有统计学意义(t=8.684~13.494,均P<0.05)。结论抑制lncRNA GAS6-AS1表达可能通过靶向miR-326/E2F1轴抑制NB细胞增殖、迁移、侵袭。 展开更多
关键词 神经母细胞瘤 长链非编码RNA生长阻滞特异性基因6反义RNA1 微小RNA-326 E2F转录因子1 增殖 迁移 侵袭
暂未订购 下载PDF
Specific RNA transcripts(SRTs):From concepts to the clinic 认领 引用
14
作者 Qili Shi Haochen Li +1 位作者 Zhiao Chen Xianghuo He 《Chinese Medical Journal》 SCIE CAS CSCD 2025年第22期2895-2906,共12页
Over the past decade,high-throughput RNA sequencing(RNA-seq)has vastly expanded our understanding of transcriptome dynamics in human physiology and disease.As a powerful tool for investigating systematic changes in RN... Over the past decade,high-throughput RNA sequencing(RNA-seq)has vastly expanded our understanding of transcriptome dynamics in human physiology and disease.As a powerful tool for investigating systematic changes in RNA biology,RNA-seq has facilitated the discovery of novel functional RNA species.Mature RNA transcripts,which transmit genetic information from DNA to proteins,undergo intricate transcriptional and post-transcriptional regulation.This process allows a single gene to produce multiple RNA transcripts,each performing specific functions depending on the physiological or pathological context.Specific RNA transcripts(SRTs)are uniquely expressed in particular tissues or tumors and are closely associated with tissue-specific functions or disease states,particularly cancer.This review explores the generation of SRTs through key mechanisms,such as alternative splicing(AS),transcriptional regulation,polyadenylation(polyA),and the influence of transposable elements(TEs).We also examine their critical roles in normal tissue development and diseases,with an emphasis on their relevance to cancer.Furthermore,the potential applications of SRTs in diagnosing and treating diseases,especially malignancies,are discussed.By serving as diagnostic markers and therapeutic targets,SRTs hold significant promise in the development of personalized medicine and precision therapies.This review aims to provide new insights into the importance of SRTs in advancing the understanding and treatment of human diseases. 展开更多
关键词 Specific RNA transcripts Cancer RNA sequencing Transposable elements Long-read sequencing
特异性蛋白1调控脂肪细胞中弗林蛋白酶表达对白脂素分泌的影响及其机制 认领 引用
15
作者 董振亚 张鑫铬 +3 位作者 邓娅娅 朱井玲 菅辉玲 张云华 《吉林大学学报（医学版）》 CAS CSCD 北大核心 2026年第2期299-307,共9页
目的:探讨肥胖状态下脂肪组织弗林蛋白酶(Furin)基因的转录调控机制及其对脂肪因子白脂素分泌的影响。方法:将24只C57BL/6J雄性小鼠随机分为正常对照饲料(NCD)组和高脂饲料(HFD)组,每组12只。连续喂养12周以构建饮食性肥胖模型。采用实... 目的:探讨肥胖状态下脂肪组织弗林蛋白酶(Furin)基因的转录调控机制及其对脂肪因子白脂素分泌的影响。方法:将24只C57BL/6J雄性小鼠随机分为正常对照饲料(NCD)组和高脂饲料(HFD)组,每组12只。连续喂养12周以构建饮食性肥胖模型。采用实时荧光定量PCR(RT-qPCR)法检测2组小鼠皮下白色脂肪组织(WAT)(sWAT)和附睾WAT(eWAT)中Furin、原纤维蛋白1(FBN1)及候选转录因子特异性蛋白1(SP1)mRNA表达水平。基于真核基因启动子收集与分析联合项目(JASPAR)数据库筛选Furin基因启动子区候选转录因子。在293T细胞中转染特异性蛋白1(SP1)过表达质粒(pcDNA-SP1组)和包含绿色荧光蛋白(GFP)的对照质粒(pcDNA-GFP组),同时将含Furin启动子区的双荧光素酶报告载体与上述质粒共转染,验证SP1与Furin启动子区之间的调控作用。分离sWAT的基质血管组分(SVF)细胞并诱导分化为成熟脂肪细胞,通过腺病毒转导过表达SP1(Ad-SP1组)或GFP对照(Ad-GFP组)。采用Western blotting法和酶联免疫吸附试验(ELISA)法分别检测Ad-GFP组和Ad-SP1组细胞中SP1和Furin蛋白表达水平及细胞上清液中白脂素水平。结果:与NCD组比较,HFD组小鼠体质量、sWAT和eWAT质量均明显升高(P<0.001)。与NCD组比较,HFD组小鼠sWAT和eWAT中Furin和SP1 mRNA表达水平明显升高(P<0.001)。JASPAR数据库预测提示SP1是调控Furin转录的核心候选因子。双荧光素酶实验,与pcDNA-GFP组比较,pcDNA-SP1组293T细胞中荧光素酶活性明显升高(P<0.001)。与Ad-GFP组比较,Ad-SP1组SVF来源的成熟脂肪细胞中SP1和Furin蛋白表达水平明显升高(P<0.001),细胞上清液中白脂素水平明显升高(P<0.001)。结论:肥胖小鼠WAT及脂肪细胞中SP1 mRNA表达上调,SP1可通过直接结合Furin启动子区增强其转录活性;SP1过表达可上调脂肪细胞中Furin蛋白表达水平,进而促进白脂素分泌。 展开更多
关键词 肥胖 弗林蛋白酶 特异性蛋白1 白脂素 转录调控
暂未订购 下载PDF
马铃薯StNST1基因克隆、亚细胞定位与表达分析 认领 引用
16
作者 汪立夏 蔡诚诚 +4 位作者 刘石锋 王涛 张宇 邹雪 王西瑶 《植物生理学报》 CAS CSCD 北大核心 2026年第5期525-534,共10页
本研究从马铃薯(Solanum tuberosum)中克隆并鉴定了调控木质素合成的基因StNST1,分析其序列特征及在不同组织中的表达模式。通过同源比对,基于已报道的拟南芥(Arabidopsis thaliana)AtNST1蛋白序列,获得了马铃薯StNST1的蛋白序列。PCR... 本研究从马铃薯(Solanum tuberosum)中克隆并鉴定了调控木质素合成的基因StNST1,分析其序列特征及在不同组织中的表达模式。通过同源比对,基于已报道的拟南芥(Arabidopsis thaliana)AtNST1蛋白序列,获得了马铃薯StNST1的蛋白序列。PCR扩增和测序结果进一步确认了StNST1的序列。生物信息学分析表明,StNST1属于NAC转录因子家族,编码区为1047 bp,编码348个氨基酸。该蛋白在N端第12至139位含有一个高度保守的NAM结构域,属于NAC(NAM/ATAF/CUC)转录因子家族的典型结构域。GO分析显示,St NST1与DNA结合(GO:0003677)及转录调控(GO:0006355)相关,表明其可能参与基因表达的调控。亚细胞定位分析表明,StNST1融合蛋白定位于细胞核和细胞质,提示其可能参与转录调控过程。系统进化分析显示,StNST1与欧白英(Solanum dulcamara)的同源蛋白具有较近的亲缘关系。为了探讨StNST1的生物学功能,采用qRT-PCR技术检测了其在不同组织中的表达模式。结果表明,StNST1在马铃薯块茎中的表达量较高,尤其在薯皮组织中的表达显著高于其他组织,提示该基因可能在块茎发育及薯皮组织结构形成过程中发挥重要作用。 展开更多
关键词 马铃薯 StNST1 NAC转录因子 亚细胞定位 组织表达分析
暂未订购 下载PDF
茉莉花WRKY基因家族全基因组鉴定及花发育表达分析 认领 引用
17
作者 赵玉磊 杨南琦 +4 位作者 梅鹏 缪媛 何悦佳 齐香玉 姜贝贝 《四川农业大学学报》 CSCD 北大核心 2026年第3期680-692,725,I0003-I0007,共13页
【目的】通过对茉莉花中WRKY基因家族进行鉴定分析,探究其在茉莉花生殖发育过程中发挥的作用。【方法】利用生物学信息分析方法,对茉莉花中WRKY基因家族成员进行理化性质、染色体定位、进化树分析、亚细胞定位预测、启动子顺式作用元件... 【目的】通过对茉莉花中WRKY基因家族进行鉴定分析,探究其在茉莉花生殖发育过程中发挥的作用。【方法】利用生物学信息分析方法,对茉莉花中WRKY基因家族成员进行理化性质、染色体定位、进化树分析、亚细胞定位预测、启动子顺式作用元件分析、基因结构和保守结构域分析以及差异基因荧光定量表达模式分析。【结果】茉莉花中鉴定出81个WRKY基因家族成员,命名为JsWRKY1~JsWRKY81,不均匀地分布在13条染色体上,除JsWRKY49位于细胞质外,其余均处于细胞核中,氨基酸数量在86~731之间不等,分子量为10511.03~81075.65 Da,等电点为4.72~10.35,均为不稳定的亲水性蛋白,具有0~5个内含子和1~6个外显子。启动子顺式作用元件包含生长发育、胁迫响应、植物激素等多个顺式作用元件,与拟南芥WRKY基因家族成员共同构建进化树分析表明,茉莉中WRKY基因家族成员在花发育等多种方面发挥作用。表达模式分析结果显示,51个JsWRKY基因在茉莉花花发育不同时期表达,57个基因在花粉-柱头互作中表达。【结论】研究鉴定出81个JsWRKY基因,荧光定量实验表明其中某些差异表达基因(JsWRKY48、JsWRKY54)在茉莉花生殖发育过程中发挥重要的作用。 展开更多
关键词 WRKY转录因子 茉莉花 生物鉴定 花发育表达分析 组织特异性
暂未订购 下载PDF
急性心肌梗死患者血清lncRNA TTTY15、HOTTIP表达及对主要不良心血管事件的预测价值 认领 引用
18
作者 李毅 刘莎 +1 位作者 吴飞妮 刘茜 《国际检验医学杂志》 CAS 2026年第10期1185-1190,1196,共6页
目的检测急性心肌梗死(AMI)患者血清lncRNA睾丸特异性转录本Y连锁15(TTTY15)、HOXA远端转录本(HOTTIP)的表达水平,研究血清TTTY15、HOTTIP对主要不良心血管事件(MACE)的预测价值。方法选取2020年6月至2023年6月安康市中心医院收治的AMI... 目的检测急性心肌梗死(AMI)患者血清lncRNA睾丸特异性转录本Y连锁15(TTTY15)、HOXA远端转录本(HOTTIP)的表达水平,研究血清TTTY15、HOTTIP对主要不良心血管事件(MACE)的预测价值。方法选取2020年6月至2023年6月安康市中心医院收治的AMI患者192例为梗死组,另选取192例该院健康体检者为健康组。梗死组患者出院后接受1年随访,根据随访结果分为MACE组和非MACE组。比较梗死组与健康组、不同Killip心功能患者的血清TTTY15、HOTTIP表达水平。收集梗死组的临床资料,比较MACE组与非MACE组的临床资料,并利用Logistic回归分析筛选梗死组患者发生MACE的影响因素。利用受试者工作特征(ROC)曲线分析血清TTTY15、HOTTIP对梗死组患者发生MACE的预测价值。结果梗死组患者血清TTTY15、HOTTIP表达水平高于健康组(P<0.05)。Ⅲ~Ⅳ级患者血清TTTY15、HOTTIP表达水平高于Ⅰ~Ⅱ级(P<0.05)。MACE组的Killip心功能Ⅲ~Ⅳ级所占比例、心肌肌钙蛋白I(cTnI)、肌酸激酶同工酶(CK-MB)、N末端脑钠肽前体(NT-proBNP)、C反应蛋白(CRP)、TTTY15、HOTTIP表达水平高于非MACE组(P<0.05)。CTnI、NT-proBNP、CRP、TTTY15、HOTTIP是AMI患者发生MACE的危险因素(P<0.05)。血清TTTY15、HOTTIP联合预测AMI患者发生MACE的AUC高于单独预测的曲线下面积(Z=4.063、3.406,均P<0.01)。结论AMI患者血清TTTY15、HOTTIP表达水平较高,是MACE发生的危险因素,联合检测TTTY15、HOTTIP可在一定程度上预测患者MACE发生。 展开更多
关键词 急性心肌梗死 睾丸特异性转录本Y连锁15 HOXA远端转录本 主要不良心血管事件
暂未订购 下载PDF
lncRNA GAS5对人心脏微血管内皮细胞缺血再灌注损伤的影响及机制实验研究 认领 引用
19
作者 范芮 马玉龙 +1 位作者 赖红梅 向阳 《陕西医学杂志》 CAS 2026年第5期605-610,共6页
目的:探讨长链非编码RNA(lncRNA)生长阻滞特异性转录因子5(GAS5)对人心脏微血管内皮细胞(HCMEC)缺血再灌注(IR)损伤的影响及其可能的机制。方法:选取HCMEC,分为正常对照组(常规培养,无干预)、模型组(建立细胞IR损伤模型)、阴性对照组(IR... 目的:探讨长链非编码RNA(lncRNA)生长阻滞特异性转录因子5(GAS5)对人心脏微血管内皮细胞(HCMEC)缺血再灌注(IR)损伤的影响及其可能的机制。方法:选取HCMEC,分为正常对照组(常规培养,无干预)、模型组(建立细胞IR损伤模型)、阴性对照组(IR+转染阴性对照慢病毒)、GAS5过表达组(IR+转染lncRNA GAS5过表达慢病毒)、GAS5干扰组(IR+转染lncRNA GAS5干扰慢病毒)。观察各组细胞形态学特征;RT-qPCR检测各组细胞GAS5 mRNA水平;ELISA法检测各组细胞炎症因子水平;MTT法检测各组细胞增殖能力;TUNEL法检测各组细胞凋亡能力;Western blot检测各组细胞微管相关蛋白1轻链3(LC3)、Beclin1蛋白表达。结果:正常对照组HCMEC贴壁紧密、形态规则、胞质均匀,无细胞脱落现象;模型组细胞贴壁能力显著下降、细胞皱缩变圆、胞质颗粒增多,且可见部分细胞从培养皿壁脱落;阴性对照组细胞形态与模型组无明显差异;GAS5过表达组上述细胞损伤相关形态学改变明显改善;GAS5干扰组细胞损伤形态较阴性对照组进一步加重。与正常对照组比较,模型组细胞凋亡率、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平升高,细胞增殖率、GAS5 mRNA及LC3、Beclin1蛋白表达水平降低(均P0.05)。GAS5过表达组上述指标变化较阴性对照组改善,而GAS5干扰组较阴性对照组加重(均P<0.05)。结论:上调lncRNA GAS5表达可减轻IR造成的HCMEC损伤,其机制可能与激活细胞自噬有关。 展开更多
关键词 长链非编码RNA生长阻滞特异性转录因子5 缺血再灌注 人心脏微血管内皮细胞 细胞增殖 细胞凋亡 自噬
暂未订购 下载PDF
多发性子宫肌瘤剔除术前后彩色多普勒超声参数、lncRNA XIST变化对术后复发评估价值 认领 引用
20
作者 陈果 王荔 +1 位作者 胡科丹 张柯 《中国妇幼健康研究》 CAS 2026年第3期100-106,共7页
目的分析多发性子宫肌瘤剔除术前后彩色多普勒超声(CDFI)参数、长链非编码RNA X染色体失活特异转录本(lncRNA XIST)变化对术后复发的评估价值。方法选取2022年1月至2024年2月泸州市人民医院收治的196例拟行多发性子宫肌瘤剔除术患者作... 目的分析多发性子宫肌瘤剔除术前后彩色多普勒超声(CDFI)参数、长链非编码RNA X染色体失活特异转录本(lncRNA XIST)变化对术后复发的评估价值。方法选取2022年1月至2024年2月泸州市人民医院收治的196例拟行多发性子宫肌瘤剔除术患者作为研究对象,根据术后6个月是否复发分为复发组和未复发组,采用倾向性评分匹配(PSM)来降低组间选择偏倚。采用Voluson E10型彩色多普勒超声诊断仪测定术前、术后1个月舒张期血流速度(EDV)、收缩期血流速度(PSV)、子宫阻力指数(UtA-RI)、子宫搏动指数(UtA-PI),采用实时荧光定量聚合酶链式反应(qRT-PCR)测定术前、术后1个月lncRNA XIST,采用Logistic回归方程分析CDFI参数、lncRNA XIST与多发性子宫肌瘤剔除术后复发的关系,采用受试者工作特征(ROC)曲线分析模型预测价值。结果术后6个月,研究对象中共有5例失访,55例(28.79%)复发;匹配后复发组术前lncRNA XIST、EDV、PSV、UtA-PI高于未复发组,UtA-RI低于未复发组,差异有统计学意义(t值介于11.733~16.918之间,P<0.05);ROC曲线显示,术前lncRNA XIST、EDV、PSV、UtA-PI、UtA-RI预测多发性子宫肌瘤剔除术后复发的曲线下面积(AUC)分别为0.754、0.766、0.792、0.776、0.742,联合预测的AUC为0.918,灵敏度为81.82%,特异度为92.73%,联合预测AUC高于单独预测(Z值介于2.215~3.340之间,P<0.001);按截断值分组,lncRNA XIST、EDV、PSV、UtA-RI、UtA-PI高值患者复发风险是低值患者的1.647、1.875、1.406、0.700、1.664倍。结论lncRNA XIST、EDV、PSV、UtA-PI、UtA-RI异常均会增加多发性子宫肌瘤剔除术后复发风险,五者联合有助于提高预测价值,指导临床诊治。 展开更多
关键词 子宫肌瘤 多发性 腹腔镜子宫肌瘤剔除术 复发 彩色多普勒超声 长链非编码RNA X染色体失活特异转录本
暂未订购 下载PDF
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
上一页 1 2 … 16 下一页 到第 页
在线咨询 使用帮助 返回顶部 意见反馈