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Noninvasive On-Skin Biosensors for Monitoring Diabetes Mellitus 认领 引用 被引量：4
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作者 Ali Sedighi Tianyu Kou +1 位作者 Hui Huang Yi Li 《Nano-Micro Letters》 SCIE EI CAS CSCD 2026年第1期375-437,共63页
Diabetes mellitus represents a major global health issue,driving the need for noninvasive alternatives to traditional blood glucose monitoring methods.Recent advancements in wearable technology have introduced skin-in... Diabetes mellitus represents a major global health issue,driving the need for noninvasive alternatives to traditional blood glucose monitoring methods.Recent advancements in wearable technology have introduced skin-interfaced biosensors capable of analyzing sweat and skin biomarkers,providing innovative solutions for diabetes diagnosis and monitoring.This review comprehensively discusses the current developments in noninvasive wearable biosensors,emphasizing simultaneous detection of biochemical biomarkers(such as glucose,cortisol,lactate,branched-chain amino acids,and cytokines)and physiological signals(including heart rate,blood pressure,and sweat rate)for accurate,personalized diabetes management.We explore innovations in multimodal sensor design,materials science,biorecognition elements,and integration techniques,highlighting the importance of advanced data analytics,artificial intelligence-driven predictive algorithms,and closed-loop therapeutic systems.Additionally,the review addresses ongoing challenges in biomarker validation,sensor stability,user compliance,data privacy,and regulatory considerations.A holistic,multimodal approach enabled by these next-generation wearable biosensors holds significant potential for improving patient outcomes and facilitating proactive healthcare interventions in diabetes management. 展开更多
关键词 Wearable biosensors Multimodal sensors Diabetes monitoring Sweat biomarkers Glucose biosensors
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Development of a whole-cell biosensor for the detection of low concentrations of tetracycline 认领 引用
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作者 Wenxiao Liu Guohao Chen +1 位作者 Yue Li Hongpo Dong 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2026年第2期640-646,共7页
By virtue of genetic engineering technology,we developed a highly sensitive biosensor for tetracycline detection based on the interaction between the tetracycline regulator TetR and tetracycline.In the absence of tetr... By virtue of genetic engineering technology,we developed a highly sensitive biosensor for tetracycline detection based on the interaction between the tetracycline regulator TetR and tetracycline.In the absence of tetracycline,TetR binds to the tetO sequence,inhibiting the expression of the sfGFP reporter gene.When tetracycline is present,it induces TetR to release from tetO,allowing sfGFP expression.The biosensor was optimized through the selection of transcription factors and reporter genes,and the optimization of spacer lengths.Chassis cells were grown to mid-log phase in a tetracycline-supplemented medium for subsequent fluorescence intensity measurement.The biosensor exhibited a strong linear correlation between fluorescence intensity and tetracycline concentration(I=37,620.7×[C(Tc)]+4048.5,R2=0.998),demonstrating high sensitivity with a detection limit of 0.0097 mg/L.The response time of the biosensor ranged from 2 to 4 h within the working concentration range,making it suitable for real-time detection.It shows potential for application in actual water sample analysis and as an early warning technology for water pollution risks. 展开更多
关键词 Whole-cell biosensors Bacterial biosensor Tetracycline repressor Tetracycline antibiotics
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A colorimetric biosensor for Escherichia coli detection in food based on aptamer-enhanced oxidase-mimicking activity of octahedral Ag2O nanoparticles 认领 引用
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作者 Xiaohuan Huang Ling Zhang +6 位作者 Tingshu Lu Jie Li Mian Wang Wei Dai Chengyin Zhou Jia Xu Yuangen Wu 《Food Innovation and Advances》 2026年第2期185-194,共10页
Escherichia coli(E.coli)poses a grave threat to food safety,underscoring the need for expeditious and precise detection methodologies.Conventional colorimetric approaches are simple,but they frequently lack accuracy a... Escherichia coli(E.coli)poses a grave threat to food safety,underscoring the need for expeditious and precise detection methodologies.Conventional colorimetric approaches are simple,but they frequently lack accuracy and stability.This study constructed an aptamer-based colorimetric biosensor,leveraging the enzyme-mimicking activity of octahedral Ag2O nanoparticles(NPs),mediated by oligonucleotides,aiming for highly sensitive foodborne E.coli detection.The P12-55 aptamer can be adsorbed onto the octahedral Ag2O NPs surface,thereby significantly enhancing their oxidase-mimicking activity.The aptamer enhances activity by promoting·O2−generation,and accelerating electron transfer to the 3,3',5,5'-tetramethylbenzidine(TMB)substrate.In the presence of E.coli in the sensing system,the aptamer exhibits a preferential binding affinity for the bacteria,thereby restoring the oxidase-mimicking activity and enabling the transduction of the detection signal.The biosensor exhibited a range of linear detection of 3×102-3×108CFU·mL−1,and the limit of detection(LOD)was as low as 4 CFU·mL−1.Recovery rates of 99.1%to 104%were achieved in milk and tap water samples,demonstrating outstanding practicality and reliability.This study proposes a novel and efficient method for the rapid detection of harmful bacteria in food,which is of significant importance for food safety assurance. 展开更多
关键词 detection methodologiesconventional Escherichia coli detection colorimetric approaches octahedral Ag O nanoparticles colorimetric biosensorleveraging oxidase mimicking activity colorimetric biosensor food safety
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A robust nonfouling electrochemical biosensor with both filtering and antifouling strategies for cortisol detection in human blood 认领 引用
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作者 Xiujuan Qiao Rui Han +3 位作者 Xinru Xu Mingrui Lv Yiting Hou Xiliang Luo 《Chinese Chemical Letters》 SCIE CAS CSCD 2026年第3期456-461,共6页
The heavy biofouling on electrochemical sensor surface poses a formidable challenge for biosensing in human blood.Herein,we designed a multilayer filtering-sensing sandwich patch that served as a versatile platform to... The heavy biofouling on electrochemical sensor surface poses a formidable challenge for biosensing in human blood.Herein,we designed a multilayer filtering-sensing sandwich patch that served as a versatile platform to surmount the substantial fouling constraints for detection in human blood.The patch integrated two functional layers:(i) Inspired by dialysis phenomenon,a filtering-mass transfer hydrophilic membrane with heterogeneous nanostructure was used to filter large-size substances(like cells,bacteria and microorganisms,etc.) and continuously pass through the rest of the biological fluid(like proteins,metabolites and inorganic salts,etc.).(ii) the polypeptide composite hydrogel(r GO/PEPG) on the screenprinted electrode(SPE) surface,with the modulation of-COOH and-NH_2 groups,endowed a strong hydrophilic layer with electric neutrality to further facilitate the antifouling ability.Notably,the integration of the filtering porous membrane with the antifouling hydrogel ensures the strong antifouling ability of the electrochemical sensor in complex human blood.Furthermore,the self-healing property of the r GO/PEPG,relying on the physical π-π stacking forces,aligns the electrochemical sensor with practical needs.The constructed antifouling biosensor based on the filtering-sensing sandwich patch was successfully applied for the sensitive detection of cortisol in human blood,with an acceptable accuracy comparable to the enzyme-linked immunosorbent assay(ELISA) method.The strategy presented herein represent a promising advance along the road to construct effective antifouling biosensing devices with robust operation in diverse complex body fluids. 展开更多
关键词 Nonfouling Electrochemical biosensor Size-sieving Polypeptide complex hydrogel Cortisol Human blood
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Recent advances in nanomaterial-based optical biosensors and their biomedical and biopharmaceutical applications 认领 引用
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作者 Mengjia Xu Lutfun Nahar +5 位作者 Kenneth J.Ritchie Chenxu Wang Li Cheng Zimiao Wu Satyajit D.Sarker Mingquan Guo 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2026年第1期54-75,共22页
Optical biosensors are gaining popularity owing to their portability,miniaturization,no requirement for additional attachments,and rapid responsiveness.These features render them suitable for various applications incl... Optical biosensors are gaining popularity owing to their portability,miniaturization,no requirement for additional attachments,and rapid responsiveness.These features render them suitable for various applications including at-home diagnostics,pharmacology,and continuous molecular monitoring.The integration of functionalized low-dimensional nanomaterials(zero-dimensional(0D),1D,2D,and 3D)has redirected focus towards the design,fabrication,and optimization of optical biosensors.This review summarizes the fundamental mechanisms underlying optical biosensing.The key mechanisms include localized surface plasmon resonance(LSPR),photoluminescence(PL),surface enhancement Raman scattering(SERS),nanozyme-based colorimetric strategies,chemiluminescence,bioluminescence,and electrochemiluminescence.The advantages of various low-dimensional nanomaterials for different types of optical biosensors are presented.This comparison emphasizes their potential superiority in targeted biosensing applications.Therefore,promoting optical biosensing techniques and recent developments in advanced biosensing strategies for biomedical research and biopharmaceutical applications are necessary to establish their future directions. 展开更多
关键词 Optical biosensors Low-dimensional nanomaterials Fundamental mechanisms for biosensing At-home diagnostics Continuous molecular monitoring Biopharmaceutical applications
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A microfluidic biosensor for rapid detection of live bacteria based on immune magnetic separation and photothermal lysis of gold nanorods 认领 引用
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作者 Nan Li Weifei Zhang +3 位作者 Gaowa Xing Zengnan Wu Xiaorui Wang Ling Lin 《Chinese Chemical Letters》 SCIE CAS CSCD 2026年第4期670-674,共5页
Foodborne bacterial infection is a serious threat to food safety,especially live pathogens causing outbreaks of most diseases.Thus developing live bacterial detection methods is important for public health.In this stu... Foodborne bacterial infection is a serious threat to food safety,especially live pathogens causing outbreaks of most diseases.Thus developing live bacterial detection methods is important for public health.In this study,a microfluidic biosensor was developed for rapid detection of live Salmonella typhimurium,in which immune magnetic particle chains in microchannels were used to separate and enrich target bacteria,antibody-conjugated gold nanorods(GNRs)were applied for photothermal lysis of target live bacteria under near-infrared(NIR)irradiation,and a photon-counting detector was used for measuring adenosine triphosphate(ATP)bioluminescence in the presence of firefly luciferin/luciferase.This biosensor was proved to be able to quantitatively detect S.typhimurium from 5.0×102 CFU/mL to 5.0×106 CFU/mL in 60 min(magnetic separation 20 min;GNRs combination 20 min;NIR irradiation,8 min)with limit of detection(LOD)of 495 CFU/mL.This biosensor showed an excellent specificity in the coexistence of other foodborne bacteria,and target bacteria were successfully measured even in the matrix interference of milk sample with mean recovery of 121.17%.This biosensor might be a promising tool for on-site assessment foodborne bacteria. 展开更多
关键词 Microfluidic biosensors Live bacteria detection Immune magnetic separation Photothermal lysis ATP bioluminescence
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In-situ growth of high-crystallinity M3(hexaaminotriphenylene)2(M=Co,Ni)thin film for field-effect transistor-based glucose biosensor 认领 引用 被引量：1
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作者 Pengwei Tan Shuyang Shen +1 位作者 Yuanyuan Luo Guotao Duan 《Chinese Chemical Letters》 SCIE CAS CSCD 2026年第4期461-466,共6页
The integration of advanced sensing materials as channel layers in devices is essential for constructing field-effect transistor(FET)biosensors.In this study,we synthesized high-crystallinity bimetallic M3(hexaamin... The integration of advanced sensing materials as channel layers in devices is essential for constructing field-effect transistor(FET)biosensors.In this study,we synthesized high-crystallinity bimetallic M3(hexaaminotriphenylene)2(M=Co,Ni)thin films as FET channel materials via an in-situ growth method using a mixed solvent system of water and N,N-dimethylformamide(DMF).This bimetallic metalorganic framework(MOF)-based FET then served as a glucose biosensor,achieving a high sensitivity and an ultra-wide detection range from 10 nmol/L to 10 mmol/L.Further studies reveal that the success of in-situ growth of the high-crystalline bimetallic MOF film can be attributed to the coordination solvent exchange reaction between the metal atomic center,DMF,and water.Furthermore,the introduction of bimetallic centers enhances the number of active sites within the MOF,thereby achieving an ultra-low detection limit and an ultra-wide detection range.This work presents a versatile approach for constructing high performance FET biosensors. 展开更多
关键词 M3(hexaaminotriphenylene)2(M=Co Ni)thin film Metal-organic framework(MOF) Field-effect transistor(FET) Glucose biosensors In-situ growth
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Highly sensitive,ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe 认领 引用
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作者 Yufang Chen Wenbo Zhao +5 位作者 Jinxing Xiong Jiashu Zhang Kai Zhang Jiale Xie Menghan Liu Hongdan Wan 《Chinese Physics B》 SCIE EI CAS CSCD 2026年第5期454-463,共10页
A highly sensitive,ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe(ROFP)is proposed and demonstrated.Our approach achieves a mass limit of detection of~... A highly sensitive,ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe(ROFP)is proposed and demonstrated.Our approach achieves a mass limit of detection of~10 aM based on the ROFP.A particular single-nucleotide mismatch sequence is also identified.The sensitivity of the proposed DNA biosensor is 1.51 nm/lgaM,about three-fold higher than using single-strand DNA probes(0.47 nm/lgaM)and with high specificity.The proposed ROFP has high compactness(with a length of~3 mm)which is convenient for sample detection with small volume and complex gradients in small spaces with high sensitivity. 展开更多
关键词 DNA biosensor ultra-low detection limit high sensitivity reflective optical fiber probe secondary interference 3-D DNA nanostructure
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Synergistic cell membrane-coated ECL-DNA biosensor for specificity-enhanced drug lead evaluation 认领 引用
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作者 Dan Wu Qi Hu +5 位作者 Qianhui Wu Guoxi Xia Jiabo Wang Yusi Bu Xiaoyu Xie Sicen Wang 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2026年第2期438-451,共14页
Cell membrane coating technology has recently emerged as a promising platform for drug activity assessment due to its unique biointerfacing capabilities.Nevertheless,its integration with conventional detection methods... Cell membrane coating technology has recently emerged as a promising platform for drug activity assessment due to its unique biointerfacing capabilities.Nevertheless,its integration with conventional detection methods such as high performance liquid chromatography(HPLC)and fluorescence probe analysis remains limited by poor specificity and low accuracy,primarily resulting from non-specific adsorption of non-target membrane receptors and interference from background signals.In this study,we presented a collaborative strategy that integrates aptamers with cell membrane coating technology to establish a novel electrochemiluminescence(ECL)-DNA biosensor platform for specifically detecting drug-target receptor interactions.High specificity was achieved through competitive binding between aptamers and drug candidates for membrane receptors,while high accuracy was ensured by employing an ECL detection system incorporating signal cascade amplification and three-dimensional(3D)DNA walkers,enabling reliable performance even with complex biological samples.Using this approach,we demonstrated a linear dynamic range of 1 nmol/L to 2μmol/L for the detection of desloratadine activity,with a limit of detection(LOD)of 0.16 nmol/L.Furthermore,the platform was successfully applied to evaluate the binding activity of eight drugs to angiotensin-converting enzyme 2(ACE2),and their pharmacological activities were further characterized.Overall,this aptamer-cell membrane coating synergistic strategy offered excellent specificity and ultra-high sensitivity,making it a valuable tool for elucidating drug-receptor mechanisms of action and providing a robust reference for preclinical drug activity evaluation. 展开更多
关键词 Cell membrane coating technology Angiotensin-converting enzyme 2 Drug lead evaluation Electrochemiluminescence biosensor Aptamer
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Performance analysis of an in-built N+pocket electrically doped TFET biosensor for biomedical applications 认领 引用
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作者 Chan Shan Qian-nan Wang Ying Liu 《Chinese Physics B》 SCIE EI CAS CSCD 2026年第2期668-678,共11页
An in-built N+pocket electrically doped tunnel field-effect transistor(ED-TFET)-based biosensor has been reported for the first time.The proposed device begins with a PN junction structure with a control gate(CG)an... An in-built N+pocket electrically doped tunnel field-effect transistor(ED-TFET)-based biosensor has been reported for the first time.The proposed device begins with a PN junction structure with a control gate(CG)and two polarity gates(PG1 and PG2).Utilizing the polarity bias concept,a narrow N+pocket is formed between the source and channel without the need for additional doping steps,achieved through biasing PG1 and PG2 at-1.2 V and 1.2 V,respectively.This method not only addresses issues related to doping control but also eliminates constraints associated with thermal budgets and simplifies the fabrication process compared to traditional TFETs.To facilitate biomolecule sensing within the device,a nanogap cavity is formed in the gate dielectric by selectively etching a section of the polarity gate dielectric layer toward the source side.The investigation into the presence of neutral and charged molecules within the cavities has been conducted by examining variations in the electrical properties of the proposed biosensor.Key characteristics assessed include drain current,energy band,and electric field distribution.The performance of the biosensor is measured using various metrics such as drain current(IDS),subthreshold swing(SS),threshold voltage(VTH),drain current ratio(ION/IOFF).The proposed in-built N+pocket ED-TFET-based biosensor reaches a peak sensitivity of 1.08×10~(13)for a neutral biomolecule in a completely filled nanogap with a dielectric constant of 12.Additionally,the effects of cavity geometry and different fill factors(FFs)on sensitivity are studied. 展开更多
关键词 electrically doped label-free biosensors PNPN tunnel field-effect transistors(TFETs) sensitivity
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Ultrasensitive biosensor based on a terahertz metasurface 认领 引用
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作者 PRADEEP TIWARI DEEPAK KALA MACIEJ SAKOWICZ 《Photonics Research》 SCIE EI CAS CSCD 2026年第5期2080-2088,共9页
Recently,metamaterial-based biosensors have shown great promise as platforms for terahertz detection that is extremely sensitive.In this work,we present a novel H-split terahertz metasurface that is enabled by structu... Recently,metamaterial-based biosensors have shown great promise as platforms for terahertz detection that is extremely sensitive.In this work,we present a novel H-split terahertz metasurface that is enabled by structural asymmetry to support a quasi-bound state in the continuum(QBIC)mode as well as an electric dipole resonance.The metasurface outperforms a number of previously published results with a sensitivity of 800 GHz/RIU on TPX and 150 GHz/RIU on silicon substrates.By shifting the central gap,a QBIC mode is created to improve sensing performance even more. 展开更多
关键词 shifting central gapa silicon substratesby ultrasensitive biosensor terahertz metasurface structural asymmetry electric dipole resonancethe terahertz detection quasi bound state continuum
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A brief review of photoactive materials for photoelectrochemical biosensors 认领 引用
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作者 Md Hayatul Islam XUE Yingxian +3 位作者 LI Min ZHANG Zuxing SANG Shengbo JIAN Aoqun 《Journal of Measurement Science and Instrumentation》 CAS CSCD 2026年第1期16-35,共20页
Photoelectrochemical(PEC)biosensors have drawn growing interest due to their capability to detect biomolecules with the help of generating photocurrent during oxidation reactions,followed by their high sensitivity,min... Photoelectrochemical(PEC)biosensors have drawn growing interest due to their capability to detect biomolecules with the help of generating photocurrent during oxidation reactions,followed by their high sensitivity,minimal background interference,costefficiency,and portability.This review provides an extensive summary of the photoactive materials that power PEC biosensor performance.We start by outlining the basic ideas and signal-generating processes of PEC biosensing,highlighting the crucial role of charge-carrier dynamics in photocurrent production.The article's main body thoroughly examines several categories of photoactive materials,such as metal oxides,quantum dots,organic materials,plasmonic nanostructures,and two-dimensional nanomaterials.We go over the special qualities,charge-transfer methods,light-harvesting capacities,and effects on biosensor performance of each material type,all supported by current experimental research.To improve sensitivity and selectivity,we also examine key design techniques,including heterojunction formation,surface functionalization,and hot-electron injection.We also discuss the main issues in PEC biosensors,including interference reduction,biocompatibility,material stability,and reproducibility.Lastly,we discuss future directions,emphasizing new materials,innovative device designs,and potential applications in food safety,environmental monitoring,and point-ofcare diagnostics.The goal of this thorough overview is to assist researchers in choosing and creating cutting-edge photoactive materials for high-performance PEC biosensors of the future. 展开更多
关键词 photo electrochemistry biosensors immunodetection sensor technology
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Engineering of l-threonine and l-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening 认领 引用 被引量：2
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作者 Wei Pu Jinhui Feng +9 位作者 Jiuzhou Chen Jiao Liu Xuan Guo Lixian Wang Xiaojia Zhao Ningyun Cai Wenjuan Zhou Yu Wang Ping Zheng Jibin Sun 《Bioresources and Bioprocessing》 SCIE EI CSCD 2025年第1期48-61,共14页
Amino acids are important bio-based products with a multi-billion-dollar market.The development of efficient high-throughput screening technologies utilizing biosensors is essential for the rapid identification of hig... Amino acids are important bio-based products with a multi-billion-dollar market.The development of efficient high-throughput screening technologies utilizing biosensors is essential for the rapid identification of high-performance amino acid producers.However,there remains a pressing need for biosensors that specifically target certain critical amino acids,such as l-threonine and l-proline.In this study,a novel transcriptional regulator-based biosensor for l-threonine and l-proline was successfully developed,inspired by our new finding that SerE can export l-proline in addition to the previously known l-threonine and l-serine.Through directed evolution of SerR(the corresponding transcriptional regulator of SerE),the mutant SerRF104I which can recognize both l-threonine and l-proline as effectors and effectively distinguish strains with varying production levels was identified.Subsequently,the SerRF104I-based biosensor was employed for high-throughput screening of the superior enzyme mutants of l-homoserine dehydrogenase and γ-glutamyl kinase,which are critical enzymes in the biosynthesis of l-threonine and l-proline,respectively.A total of 25 and 13 novel mutants that increased the titers of l-threonine and l-proline by over 10%were successfully identified.Notably,six of the newly identified mutants exhibited similarities to the most effective mutants reported to date,indicating the promising application potential of the SerRF104I-based biosensor.This study illustrates an effective strategy for the development of transcriptional regulator-based biosensors for amino acids and other chemical compounds. 展开更多
关键词 L-Threonine and l-proline biosensor Transcriptional regulator SerR Directed evolution High-throughput screening l-Homoserine dehydrogenase γ-Glutamyl kinase
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Application of CRISPR-Cas System in Human Papillomavirus Detection Using Biosensor Devices and Point-of-Care Technologies 认领 引用 被引量：1
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作者 Chang He Yongqi Li +6 位作者 Jinkuan Liu Zhu Li Xue Li Jeong-Woo Choi Heng Li Shan Liu Chen-zhong Li 《Biomedical Engineering Frontiers》 EI CAS 2025年第1期392-411,共20页
Human papillomavirus(HPV)is the most common virus for genital tract infections.Cervical cancer ranks as the fourth most prevalent cancer globally,with over 99%of cases in women attributed to HPV infection.This infecti... Human papillomavirus(HPV)is the most common virus for genital tract infections.Cervical cancer ranks as the fourth most prevalent cancer globally,with over 99%of cases in women attributed to HPV infection.This infection continues to pose an ongoing threat to public health.Therefore,the development of rapid,high-throughput,and sensitive HPV detection platforms is important,especially in regions with limited access to advanced medical resources.CRISPR-based biosensors,a promising new method for nucleic acid detection,are now rapidly and widely used in basic and applied research and have received much attention in recent years for HPV diagnosis and treatment.In this review,we discuss the mechanisms and functions of the CRISPR-Cas system,focusing on its applications in HPV diagnostics.The review covers CRISPR technologies such as CRISPR-Cas9,CRISPR-Cas12,and CRISPR-Cas13,along with nucleic acid amplification methods,CRISPR-based signal output systems,and point-of-care testing(POCT)strategies.This comprehensive overview highlights the versatility and potential of CRISPR technologies in HPV detection.We also discuss the numerous CRISPR biosensors developed since the introduction of CRISPR to detect HPV.Finally,we discuss some of the challenges faced in HPV detection by the CRISPR-Cas system. 展开更多
关键词 nucleic acid det genital tract infectionscervical cancer biosensor devices point care technologies nucleic acid amplification human papillomavirus detection CRISPR Cas system human papillomavirus hpv
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Genetically encoded biosensors for spatiotemporal monitoring of plantproteins in growth and stress responses 认领 引用 被引量：1
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作者 Han Xiao Min Li +1 位作者 Nir Ohad Ge-Fei Hao 《Advanced Agrochem》 CAS CSCD 2025年第3期177-187,共11页
Genetically encoded biosensors are powerful tools for monitoring plant proteins,which could offer high spatial and temporal resolution and help reveal the molecular mechanisms underlying plant growth and stress respon... Genetically encoded biosensors are powerful tools for monitoring plant proteins,which could offer high spatial and temporal resolution and help reveal the molecular mechanisms underlying plant growth and stress responses.However,a comprehensive review focused on the spatiotemporal monitoring of plant proteins using these biosensors is still lacking.This review highlights key advancements in the field,evaluates the strengths and limitations of current biosensors,and discusses their applications for tracking plant protein dynamics.We aim to provide a thorough understanding of genetically encoded biosensors for plant proteins,promote the development of these technologies,and foster deeper insights into molecular mechanisms in plant cells.Future research should prioritize overcoming challenges such as interference from plant autofluorescence and enhancing the sensitivity of biosensors,particularly in complex cellular compartments like chloroplasts and cell walls,to further improve spatial and temporal resolution. 展开更多
关键词 Genetically encoded biosensors Protein activity Protein-protein interaction Plant growth and development Stress response
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High-performance Fructosyl Amino Acid Oxidase Biosensor Based on Polyaniline/Polyindole-Mn2O3 Nanofibers for Rapid Monitoring of Diabetes Mellitus 认领 引用
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作者 Nasim Kharazmi Nejad Amir Amirabadi +1 位作者 Laleh Maleknia Hassan Tavakoli 《Journal of Bionic Engineering》 SCIE EI CSCD 2025年第6期3154-3173,共20页
Hemoglobin A1c(HbA1c),a key biomarker for long-term glucose regulation,is essential for diagnosing and managing diabetes mellitus.However,conventional HbA1c detection methods often suffer from limited sensitivity,narr... Hemoglobin A1c(HbA1c),a key biomarker for long-term glucose regulation,is essential for diagnosing and managing diabetes mellitus.However,conventional HbA1c detection methods often suffer from limited sensitivity,narrow detection ranges,slow response times,and poor long-term stability.In this study,we developed a high-performance amperometric biosensor for the selective detection of Fructosyl Valine(FV),a model compound for HbA1c,by immobilizing Fructosyl Amino Acid Oxidase(FAAO)onto a glassy carbon electrode modified with electrospun polyaniline/polyindole-Mn2O3 nanofibers.Operating at an applied potential of 0.27 V versus Ag/AgCl,the biosensor achieved a rapid detection time of 2 s for FV concentrations up to 50µM,with a signal-to-noise ratio of 3.Under optimized conditions(pH 7.0 and 35℃),the biosensor exhibited a wide linear detection range from 0.1 to 3 mM and a high sensitivity of 38.42µA/mM.Importantly,the sensor retained approximately 70% of its initial activity after 193 days of storage at 4℃,demonstrating excellent long-term stability.These results suggest that the FAAO/polyaniline/polyindole-Mn2O3 nanocomposite-based biosensor offers a promising platform for sensitive,rapid,and durable detection of HbA1c,providing significant potential for improving diabetes monitoring and management. 展开更多
关键词 Biosensor Electrospun nanofibers Enzymatic immobilization HbA1c Polyaniline Polyindole Diabetes monitoring
Simultaneous detection of inflammatory process indicators via operando dual lossy mode resonance-based biosensor 认领 引用
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作者 Desiree Santano Abian B.Socorro +2 位作者 Ambra Giannetti Ignacio Del Villar Francesco Chiavaioli 《Opto-Electronic Science》 CAS 2025年第10期14-29,共16页
Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An ... Detecting multiple analytes simultaneously,crucial in disease diagnosis and treatment prognosis,remains challenging.While planar sensing platforms demonstrate this capability,optical fiber sensors still lag behind.An operando dual lossy mode resonance(LMR)biosensor fabricated on a D-shaped single-mode fiber(SMF)is proposed for quantification of clinical indicators of inflammatory process,like in COVID-19 infection.Dual LMRs,created via two-step deposition process,yield a nanostructure with distinct SnO2 thicknesses on the flat surface of the fiber.Theoretical and experimental analyses confirm its feasibility,showing a sensitivity around 4500 nm/RIU for both LMRs.A novel insight in spatially-separated biofunctionalization of the sensitive fiber regions is validated through fluorescence assays,showcasing selectivity for different immunoglobulins.Real-time and label-free detection of two inflammatory markers,C-reactive protein and Ddimer,empowers the platform capability with a minimum detectable concentration below 1μg/mL for both biomolecules,which is of clinical interest.This proof-of-concept work provides an important leap in fiber-based biosensing for effective and reliable multi-analyte detection,presenting a novel,compact and multi-functional analytical tool. 展开更多
关键词 fiber optic biosensors multi-analyte detection inflammatory process indicators dual lossy mode resonance(LMR) label-free detection
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Plasmonic dual-parameter biosensor with self-referencing capability based on one-dimensional metal gratings 认领 引用
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作者 LIU Jun LUO Jianhua 《Optoelectronics Letters》 EI 2025年第8期449-454,共6页
There is limited amount of research on surface plasmon resonance(SPR)sensors with self-referencing capabilities which are based on dielectric gratings.In the short-wavelength range,a metal grating sensor is capable of... There is limited amount of research on surface plasmon resonance(SPR)sensors with self-referencing capabilities which are based on dielectric gratings.In the short-wavelength range,a metal grating sensor is capable of simultaneously measuring liquid refractive index under proposed temperature.A fabricated gold grating is placed on one side of a thin gold film for refractive index measurement,while the other with polydimethylsiloxane(PDMS)is deposited on the other side for temperature measurement.We use finite element analysis to research its sensing characteristics.Due to the high refractive index sensitivity of SPR sensors and thermo-optic coefficient of PDMS,we discovered the maximum spectral sensitivity of the sensor is 564 nm/RIU and-50 pm/℃when the liquid refractive index ranges from 1.30 to 1.40 with temperature ranging from 0℃ to 100℃.Numerical results indicate that there may not be mutual interference between two channels for measuring refractive index and temperature,which reduces the complexity of sensor measurements. 展开更多
关键词 thin gold film fabricated gold grating plasmonic biosensor metal grating sensor liquid refractive index self referencing capability refractive index dielectric gratingsin
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Biosensors: Types, features, and application in biomedicine 认领 引用
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作者 Elmira Karami Fatemeh Kazemi-Lomedasht 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2022年第9期367-373,共7页
Fast and precise diagnostic techniques are required for the treatment of many disorders.Biosensors are one of the diagnostic devices that are applicable in biological and medical sciences.Biosensors could be utilized ... Fast and precise diagnostic techniques are required for the treatment of many disorders.Biosensors are one of the diagnostic devices that are applicable in biological and medical sciences.Biosensors could be utilized to recognize biological molecules with high sensitivity.Biosensors are consisted of different components and have different types.Each type of biosensor is used in a particular field according to its specific features.Nanobodies are a novel class of antibodies with small size,high affinity,and specificity to their target.The unique properties of nanobodies make them appropriate tools for diagnostic applications.In this paper,we review biosensors,and their features and roles in medicine.Antibodyanobody-based biosensors are also specifically discussed. 展开更多
关键词 Biosensor Nanobody-based biosensor Antibodybased biosensor Biomedicine Biotechnology
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Recent advances in the design of biosensors based on novel nanomaterials:An insight 认领 引用 被引量：5
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作者 Nidhi Chauhan Kirti Saxena +1 位作者 Mayukh Tikadar Utkarsh Jain 《Nanotechnology and Precision Engineering》 EI CAS CSCD 2021年第4期71-88,共18页
Biosensors have acquired much importance in drug discovery,medical diagnostics,food safety,defense,security,and monitoring of environmental conditions.Furthermore,there has been great progress in the potential applica... Biosensors have acquired much importance in drug discovery,medical diagnostics,food safety,defense,security,and monitoring of environmental conditions.Furthermore,there has been great progress in the potential applications of advanced nanomaterials in biosensors.Every year there are several advances in sensing techniques that can be attributed to nanomaterials,biorecognition elements,or their related fabrication techniques.The further development of nanotechnology-based sensors provides a wide variety of opportunities to modern research.Advanced nanomaterials can provide remarkable optical,electrical,mechanical,and catalytic properties.For example,transition metals and organic polymers have been used in the fabrication of powerful,sensitive,and precise biosensors.The distinctive properties of advanced nanomaterials have been widely incorporated into biosensors.However,fabrication techniques also play important roles in the development of these devices.Therefore,we present a review of some of the advanced nanomaterials that have been widely used over the last few years and discuss their fabrication techniques.The focus of this review is to provide a directional perspective of recently fabricated advanced nanomaterial-based biosensors in the diagnosis of various diseases. 展开更多
关键词 Biosensors Advanced nanomaterials Transition metals Fabrication of biosensors Fabrication techniques
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