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Taurochenodeoxycholic acid promotes abdominal fat deposition by modulating the crosstalk between bile acid metabolism and intestinal microbiota in broilers 认领 引用
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作者 Xi Sun Chaohui Wang +4 位作者 Xiaoying Liu Yun Li Zhouzheng Ren Xiaojun Yang Yanli Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期531-545,共15页
Background The role of bile acids in modulating the gut microbiota and their impact on host metabolism has garnered significant attention.Taurochenodeoxycholic acid(TCDCA)is the predominant bile acid within the chicke... Background The role of bile acids in modulating the gut microbiota and their impact on host metabolism has garnered significant attention.Taurochenodeoxycholic acid(TCDCA)is the predominant bile acid within the chicken bile acid pool and is closely related to metabolic disorders.The current study aims to investigate the potential effects of TCDCA on abdominal fat deposition in broilers.From 14 to 28 days of age,the broilers in the CON group received an oral administration of 1 mL of saline,while those in the treatment groups were administered 1 mL of a solution containing 0.05 g,0.10 g,or 0.20 g of TCDCA.Results The results showed that TCDCA treatments from 14 to 28 d had no significant effects on BW,ADFI,ADG and FCR in broilers at the age of 28 days of age.However,the abdominal fat percentage in the 0.20 g TCDCA group significantly increased,accompanied by higher TBA and HDL-c levels,as well as a reduction in apolipoprotein B levels in serum.In addition,serum triglyceride levels tended to be higher in the 0.20 g TCDCA group(P=0.098).The 0.20 g TCDCA treatment increased the gene expressions of SREBP-1,C/EBP-α,and ELOVL6,while decreasing the mRNA abundance of ATGL and CPT-1 in the abdominal fat.Serum levels of TCDCA,TDCA,and THDCA were significantly higher after 0.20 g TCDCA administration,while TCA levels were significantly lower,as determined by the targeted bile acid metabolomics analysis.Conversely,hepatic mRNA levels of CYP7A1,CYP27A1,BAAT,and BSEP were increased in the 0.20 g TCDCA group.The oral administration of 0.20 g TCDCA also upregulated the expression of FXR,VDR,and FGF19 in abdominal fat.The 16S rRNA analysis of cecal microbiota revealed that a decrease in the Shannon and Simpson indexes in the 0.20 g TCDCA group,and an increase in the Firmicutes/Bacteroidetes ratio.LEfSe analysis revealed that the predominant bacteria in the CON group were Streptococcus and Oscillospira at the genus level,while Lactobacillus,Parabacteroides,Anaeroplasma,and Helicobacter were identified as the dominant genera in the 0.20 g TCDCA group.Functional predictions for the gut microbiota exhibited that lipid metabolism,replication and repair pathway were enhanced in the 0.20 g TCDCA group.Correlation analysis demonstrated that the abundance of Lactobacillus was positively correlated with serum levels of TCDCA,THDCA,and TDCA,while the abundance of Streptococcus and Oscillospira showed a positive correlation with serum TCA levels.Conclusion Overall,this study elucidates that the intervention of 0.20 g TCDCA may promote abdominal fat deposition by activating bile acid receptors in abdominal fat,and concurrent alterations in both the intestinal microbial community and bile acid profile. 展开更多
关键词 Abdominal fat Broiler Cecal microbiota Taurochenodeoxycholic acid
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Testing low dietary crude protein and high fat levels as a strategy to mitigate heat stress in broilers 认领 引用
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作者 Renée De Baets Sofie Van Nerom +3 位作者 Kobe Buyse Gunther Antonissen Jeroen Degroote Evelyne Delezie 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第1期566-582,共17页
Background Fast-growing broilers are poorly adapted to heat.Adjusting feed composition may mitigate heat stress(HS)effects in temperate climates,while maintaining performance and health during cooler days.Methods One ... Background Fast-growing broilers are poorly adapted to heat.Adjusting feed composition may mitigate heat stress(HS)effects in temperate climates,while maintaining performance and health during cooler days.Methods One thousand nine hundred and twenty Ross 308 male broilers were housed in 64 pens in 4 climate-controlled rooms,2 under cyclical HS(d 28–43;32±2℃;60%–70%RH;09:30–15:30)and 2 under thermoneutral(TN)conditions.In the finisher phase,broilers were allocated to 4 dietary treatments,analyzed values are given except for metabolizable energy(ME):low crude protein(CP)and control fat(LowCP-ConF;17.0%CP,5.9%crude fat(CF),2,925 kcal/kg ME),low CP and high fat(LowCP-HighF;17.2%CP,7.9%CF,3,019 kcal/kg ME),control CP and high fat(ConCP-HighF;18.1%CP,8.0%CF,2,992 kcal/kg ME)and a basal control(ConCP-ConF;18.7%CP,6.3%CF,2,913 kcal/kg ME).LowCP diets contained control levels of digestible amino acids.Results During the finisher phase,compared to control CP levels,LowCP increased average daily feed intake(ADFI)(+2.15%;P=0.020)and affected average daily gain(ADG)and feed conversion ratio(FCR)negatively under TN(-3.77%and+6.49%;P=0.003 and P<0.001,respectively),but not during HS.Compared to control CF,HighF decreased ADFI during TN and HS(-3.16%and-3.17%;P<0.001 and P=0.022)and reduced ADG in TN groups(-3.17%;P=0.010),but not during HS.Mortality was higher in broilers receiving HighF during HS(P=0.040).Slaughter weights were unaffected.LowCP decreased plasma uric acid and lactate dehydrogenase levels during TN,but increased plasma glucose during HS.LowCP increased breast meat redness(a*)during TN and HS(P<0.05).HighF decreased fat(-1.68%;P=0.017),but increased protein levels(+1.53%;P<0.001)in breast meat of HS-broilers.Conclusion LowCP and HighF impaired performance under TN but not under HS.HighF increased mortality under HS,yet improved breast meat composition.These findings highlight the challenge of designing an optimal diet for both conditions and underscore the need to better understand amino acid needs and energy-to-protein ratios during HS. 展开更多
关键词 Broiler Crude fat Crude protein Energy Heat stress
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Bacillus velezensis mitigates chronic LPS-induced lung injury of broilers via microbiota-driven isoflavone production and NF-κB/PPAR-γaxis modulation 认领 引用
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作者 Xunbozan Zhang Xuan Liu +5 位作者 Ye Xu Dadan Liu Ying Chen Christopher Peterson Daniel Ruixiao Mao Liang Chen 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2026年第4期2195-2214,共20页
Background Chronic exposure to low-dose lipopolysaccharide(LPS)in poultry farming environments induces persistent respiratory inflammation,resulting in lung injury and impaired growth performance in broilers.Bacillus ... Background Chronic exposure to low-dose lipopolysaccharide(LPS)in poultry farming environments induces persistent respiratory inflammation,resulting in lung injury and impaired growth performance in broilers.Bacillus velezensis(BV)is a probiotic with known antibacterial and immunomodulatory activities,yet its role in respiratory health remains poorly understood.This study aimed to assess the potential benefits of BV in alleviating chronic pneumonia triggered by LPS in broilers and to clarify its mechanistic pathways.Results A chronic LPS intratracheal instillation model was established,comprising control,LPS,and BV+LPS groups.BV supplementation significantly ameliorated LPS-induced growth impairment(P<0.05),inhibited the synthesis of key inflammatory mediators,and mitigated oxidative stress in serum and bronchoalveolar lavage fluid(P<0.05).Integrated multi-omics analyses revealed that BV remodeled the pulmonary microbiota,enriching isoflavone-metabolizing taxa including Blautia and unclassified Lachnospiraceae(P<0.05),which was associated with elevated pulmonary concentrations of daidzein,genistein,and glycitein(P<0.05).Transcriptomic together with molecular analyses revealed that BV enhanced the activation of PPAR-γwhile attenuating NF-κB pathway activity,thereby reducing the expression of genes associated with inflammation(P<0.05).In vitro,experiments showed that daidzein and genistein inhibited cellular inflammatory responses through PPAR-γsignaling.BV culture supernatant directly suppressed NF-κB/NLRP3 inflammasome activation in chicken HD11 macrophages,reduced intracellular reactive oxygen species(ROS)generation,and shifted macrophage polarization toward an anti-inflammatory phenotype(P<0.05).Conclusion These findings demonstrate that BV alleviates LPS-induced chronic pneumonia through two complementary pathways,as it remodels the pulmonary microbiota to enhance isoflavone metabolism and thereby suppress inflammation,while its own metabolites also directly inhibit inflammatory signaling.This study provides new insight into probiotic-based interventions for respiratory health in livestock. 展开更多
关键词 Bacillus velezensis Broiler lung Chronic inflammation Isoflavone Lipopolysaccharide NF-κB
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Research progress on plant flavonoids as a strategy against oxidative stress in broilers:mechanistic insights and the potential of Silphium perfoliatum L.:review 认领 引用
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作者 Kabelo Anthony Makatjane Haizhu Zhou 《Animal Diseases》 CAS CSCD 2026年第2期197-225,共29页
The broiler industry faces an urgent demand for the development of sustainable and antibiotic-free methods to com-bat widespread environmental and metabolic stressors,resulting in decreased performance of birds,compro... The broiler industry faces an urgent demand for the development of sustainable and antibiotic-free methods to com-bat widespread environmental and metabolic stressors,resulting in decreased performance of birds,compromised welfare,and substantial financial losses.This complete review focuses on the use of plant flavonoids(PFs)as a mul-ticomponent antistress approach to address the common outcomes of all these stressors:systemic oxidative stress and gut barrier dysfunction.Through the detailed evaluation of over 300 scientific references,this review has demon-strated that PFs have dual-signaling pathway actions to protect animals against these stresses.The first action involves powerful antioxidant activity via the activation of the Keap1-Nrf2 signaling pathway,leading to the upregulation of phase II detoxification enzymes and cytoprotective enzymes(GPX and GST)to restore the cellular redox state.These enhanced defense systems are critical for protecting cells against chronic heat stress and for providing clear-ance mechanisms against highly toxic mycotoxins(Aflatoxin B1;Ochratoxin A).The second action of PFs is strong anti-inflammatory protection via the inhibition of the antagonistic NF-κB signaling pathway,which attenuates proin-flammatory cytokine storms.This attenuation directly supports intestinal barrier function by promoting the expres-sion of tight junction proteins in response to both heat stress and enterotoxic mycotoxins(T-2 toxin),thus preventing leaky gut syndrome.Additionally,PFs can be used to mitigate acute physiological challenges.By modulating the HPA axis,they can reduce the circulating levels of stress hormones(corticosterone/cortisol)in birds subjected to han-dling and transportation stress.Finally,the antioxidant properties of PFs penetrate muscle tissue,thereby preventing oxidation and stabilizing the myofibrillar protein structure postmortem.This action results in higher-quality poultry meat with lower drip loss.Although specific flavonoid compounds such as those found in Silphium perfoliatum L.may hold promise,the real value of PFs lies in their ability to act via these common downstream pathways(Nrf2/NF-kB)to position them as emerging,sustainable alternatives.Therefore,future research should prioritize controlled feeding trials and cost‒benefit analyses to quickly develop PF-based feed additives that improve global growth performance,decrease mortality,and increase the quality of poultry meat. 展开更多
关键词 Plant Flavonoids Broiler Chicken Oxidative Stress Nrf2/NF-κB Heat Stress Mycotoxin Detoxification Gut Barrier Function Neuroendocrine Modulation Meat Quality Feed Additives
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Improvement of antioxidant capability by dietary N‑acetyl cysteine supplementation alleviates bone loss induced by chronic heat stress in finisher broilers 认领 引用 被引量：2
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作者 Huaiyong Zhang Herinda Pertiwi +7 位作者 Joris Michiels Djoere Gaublomme Maryam Majdeddin Yuhuang Hou Matthieu Boone Dirk Elewaut Ivan Josipovic Jeroen Degroote 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第2期777-796,共20页
Background Heat stress(HS) incidence is associated with the accumulation of reactive substances, which might be associated with bone loss. N-Acetylcysteine(NAC) exhibits strong antioxidants due to its sulfhydryl group... Background Heat stress(HS) incidence is associated with the accumulation of reactive substances, which might be associated with bone loss. N-Acetylcysteine(NAC) exhibits strong antioxidants due to its sulfhydryl group and being as the precursor for endogenous glutathione synthesis. Therefore, interplay between oxidative stress and bone turnover of broilers and the effects of dietary NAC inclusion on antioxidant capability and “gut-bone” axis were evaluated during chronic HS.Results Implementing cyclic chronic HS(34 ℃ for 7 h/d) evoked reactive oxygen species excessive production and oxidant stress, which was accompanied by compromised tibia mass. The RNA-seq of proximal tibia also revealed the enrichment of oxidation–reduction process and inflammatory outbursts during HS. Although no notable alterations in the growth performance and cecal microbiota were found, the diet contained 2 g/kg NAC enhanced the antioxidant capability of heat-stressed broiler chickens by upregulating the expression of Nrf2 in the ileum, tibia, and bone marrow. Simultaneously, NAC tended to hinder NF-κB pathway activation and decreased the m RNA levels of the proinflammatory cytokines in both the ileum and bone marrow. As a result, NAC suppressed osteoclastogenesis and osteoclast activity, thereby increasing osteocyte-related gene expression. Furthermore, the inclusion of NAC tended to increase the ash content and density of the whole tibia, as well as improve cortical thickness and bone volume of the diaphysis.Conclusions These findings HS-mediated outburst of oxidant stress accelerates bone resorption and negatively regulates the bone quality of tibia, which is inhibited by NAC in broilers. 展开更多
关键词 Bone mass Broilers Heat stress Intestine N-Acetylcysteine Oxidative stress
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The ameliorative role of phlorotannin on aflatoxin B1‑induced liver oxidative stress and mitochondrial injury is related to the activation of Nrf2 and Nrf1 signaling pathways in broilers 认领 引用 被引量：2
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作者 Xueqing Ye Yuying Yang +4 位作者 Qinghua Yao Mengyi Huang Balamuralikrishnan Balasubramanian Rajesh Jha Wenchao Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2195-2212,共18页
Background Aflatoxin B1(AFB1)risks animal and human health,and the liver is considered the most crucial detoxification organ.Phlorotannin(PT)is a polyhydroxy phenol that has a wide range of biological activities... Background Aflatoxin B1(AFB1)risks animal and human health,and the liver is considered the most crucial detoxification organ.Phlorotannin(PT)is a polyhydroxy phenol that has a wide range of biological activities,including antioxidation and hepatoprotection,which can promote the ability of liver detoxification.This study aimed to elucidate the protective effect of PT on AFB1-induced liver damage in broilers.Results In vivo experiment showed that the PT reduced AFB1 content and AFB1-exo-8,9-epoxide DNA(AFBODNA)concentration in serum and liver(P<0.05),improved the histomorphology of liver and hepatic mitochondria,and activated nuclear factor erythroid 2-related factor 2(Nrf2)-related antioxidant and detoxification pathway by upregulating the activities of antioxidant enzymes(catalase[CAT],glutathione S-transferase[GST])and total antioxidant capacity(T-AOC)level(P<0.05),and inhibited the mRNA expression of CYP1A1(cytochrome P450 family 1 subfamily A member 1)and phase Ⅱ detoxification enzyme related genes(GPX1,GSTT1,and NQO1)of broilers exposed to AFB1(P<0.05).Meanwhile,PT upregulated the Nrf1 pathway-related mitochondrial biosynthetic genes(Nrf1,mitochondrial transcription factor A[TFAM],mitofusin 1[MFN1])in broilers fed AFB1 contaminated diet(P<0.05).In vitro verification study suggested that the use of Nrf2/Nrf1 inhibitors suppressed the ameliorative role of PT on AFB1-induced liver injury of broilers,which was manifested in the mRNA expression of Nrf2,NQO1,GSTT3,Nrf1,TFAM,and other genes decreasing(P<0.05),and down-regulation of the protein expression of Nrf2,total and nucleus p-Nrf2,and total and nucleus p-Nrf1(P<0.05).Conclusion The PT ameliorates oxidative stress and hepatotoxicity by activating the Nrf2-mediated phase Ⅱ detoxification enzymes pathway and maintains mitochondrial homeostasis by activating the Nrf1 signaling pathway in broilers exposed to AFB1. 展开更多
关键词 Aflatoxin B1 Biological detoxification Broiler chickens Liver injury Phlorotannin
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Dihydrosanguinarine enhances tryptophan metabolism and intestinal immune function via AhR pathway activation in broilers 认领 引用 被引量：1
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作者 Yue Su Miaomiao Wang +2 位作者 Zhiyong Wu Peng Huang Jianguo Zeng 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第5期2322-2340,共19页
Background Tryptophan is essential for nutrition,immunity and neural activity,but cannot be synthesized endogenously.Certain natural products influence host health by modulating the gut microbiota to promote the produ... Background Tryptophan is essential for nutrition,immunity and neural activity,but cannot be synthesized endogenously.Certain natural products influence host health by modulating the gut microbiota to promote the production of tryptophan metabolites.Sanguinarine(SAN)enhances broiler immunity,however,its low bioavailability and underlying mechanisms remain unclear.This study aimed to decode the mechanisms by which sanguinarine enhances intestinal immune function in broilers.Methods Liquid chromatography-tandem mass spectrometry(LC-MS/MS)was employed to identify the main metabolites of sanguinarine in the intestine.Subsequently,equal concentrations of sanguinarine and its metabolites were separately added to the diets.The effects of sanguinarine and its metabolites on the intestinal immune function of broiler chickens were evaluated using 16S rRNA gene amplicon sequencing and tryptophan metabolomics approaches.Results We determined that dihydrosanguinarine(DHSA)is the main metabolite of sanguinarine in the intestine.Both compounds increased average daily gain and reduced feed efficiency,thereby improving growth performance.They also enhanced ileal villus height and the villus-to-crypt(V/C)ratio while decreasing crypt depth and upregulating the mRNA expression of tight junction proteins ZO-1,occludin and claudin-1.Furthermore,both compounds promoted the proliferation of intestinal Lactobacillus species,a tryptophan-metabolizing bacterium,stimulated short-chain fatty acid production,and lowered intestinal pH.They regulated tryptophan metabolism by increasing the diversity and content of indole tryptophan metabolites,activating the aryl hydrocarbon receptor(AhR)pathway,and elevating the mRNA levels of CYP1A1,CYP1B1,SLC3A1,IDO2 and TPH1.Inflammatory cytokines IL-1β and IL-6 were inhibited,while anti-inflammatory cytokines IL-10 and IL-22,serum SIgA concentration,and intestinal MUC2 expression were increased.Notably,DHSA exhibited a more pronounced effect on enhancing immune function compared to SAN.Conclusions SAN is converted to DHSA in vivo,which increases its bioavailability.DHSA regulates tryptophan metabolism by activating the AhR pathway and modulating immune-related factors through changes in the gut microbiota.Notably,DHSA significantly increases the abundance of Lactobacillus,a key tryptophan-metabolizing bacterium,thereby enhancing intestinal immune function and improving broiler growth performance. 展开更多
关键词 Broiler Gut microbiota Intestinal immunity Sanguinarine Tryptophan metabolism
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Dietary bile acids supplementation protects against Salmonella Typhimurium infection via improving intestinal mucosal barrier and gut microbiota composition in broilers 认领 引用 被引量：1
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作者 Dan Hu Xiaoran Yang +4 位作者 Ming Qin Li’an Pan Haiyan Fang Pengnan Chen Yingdong Ni 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第2期695-713,共19页
Background Salmonella Typhimurium(S.Typhimurium)is a common pathogenic microorganism and poses a threat to the efficiency of poultry farms.As signaling molecules regulating the interaction between the host and gut mic... Background Salmonella Typhimurium(S.Typhimurium)is a common pathogenic microorganism and poses a threat to the efficiency of poultry farms.As signaling molecules regulating the interaction between the host and gut microbiota,bile acids(BAs)play a protective role in maintaining gut homeostasis.However,the antibacterial effect of BAs on Salmonella infection in broilers has remained unexplored.Therefore,the aim of this study was to investigate the potential role of feeding BAs in protecting against S.Typhimurium infection in broilers.Methods A total of 1441-day-old Arbor Acres male broilers were randomly assigned to 4 groups,including non-challenged birds fed a basal diet(CON),S.Typhimurium-challenged birds(ST),S.Typhimurium-challenged birds treated with 0.15 g/kg antibiotic after infection(ST-ANT),and S.Typhimurium-challenged birds fed a basal diet supplemented with 350 mg/kg of BAs(ST-BA).Results BAs supplementation ameliorated weight loss induced by S.Typhimurium infection and reduced the colonization of Salmonella in the liver and small intestine in broilers(P<0.05).Compared to the ST group,broilers in ST-BA group had a higher ileal mucosal thickness and villus height,and BAs also ameliorated the increase of diamine oxidase(DAO)level in serum(P<0.05).It was observed that the mucus layer thickness and the number of villous and cryptic goblet cells(GCs)were increased in the ST-BA group,consistent with the upregulation of MUC2 gene expression in the ileal mucosa(P<0.05).Moreover,the m RNA expressions of Toll-like receptor 5(TLR5),Toll-like receptor 4(TLR4),and interleukin 1 beta(IL1b)were downregulated in the ileum by BAs treatment(P<0.05).16S rDNA sequencing analysis revealed that,compared to ST group,BAs ameliorated the decreases in Bacteroidota,Bacteroidaceae and Bacteroides abundances,which were negatively correlated with serum DAO activity,and the increases in Campylobacterota,Campylobacteraceae and Campylobacter abundances,which were negatively correlated with body weight but positively correlated with serum D-lactic acid(D-LA)levels(P<0.05).Conclusions Dietary BAs supplementation strengthens the intestinal mucosal barrier and reverses dysbiosis of gut microbiota,which eventually relieves the damage to the intestinal barrier and weight loss induced by S.Typhimurium infection in broilers. 展开更多
关键词 Bile acid Broiler Intestinal barrier Gut microbiota Salmonella Typhimurium
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Dietary Zn proteinate with moderate chelation strength alleviates heat stress‑induced intestinal barrier function damage by promoting expression of tight junction proteins via the A20/NF‑κB p65/MMP‑2 pathway in the jejunum of broilers 认领 引用 被引量：1
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作者 Yangyang Hu Weiyun Zhang +12 位作者 Ke Yang Xi Lin Hsiao‑Ching Liu Jack Odle Miles Todd See Xiaoyan Cui Tingting Li Shengchen Wang Xiudong Liao Liyang Zhang Sufen Li Yun Hu Xugang Luo 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期316-330,共15页
Background The aim of this study was to determine whether and how Zn proteinate with moderate chelation strength(Zn-Prot M)can alleviate heat stress(HS)-induced intestinal barrier function damage of broilers.A complet... Background The aim of this study was to determine whether and how Zn proteinate with moderate chelation strength(Zn-Prot M)can alleviate heat stress(HS)-induced intestinal barrier function damage of broilers.A completely randomized design was used for comparatively testing the effects of Zn proteinate on HS and non-HS broilers.Under high temperature(HT),a 1(Control,HT-CON)+2(Zn source)×2(added Zn level)factorial arrangement of treatments was used.The 2 added Zn sources were Zn-Prot M and Zn sulfate(ZnS),and the 2 added Zn levels were 30 and 60 mg/kg.Under normal temperature(NT),a CON group(NT-CON)and pair-fed group(NT-PF)were included.Results The results showed that HS significantly reduced mRNA and protein expression levels of claudin-1,occludin,junctional adhesion molecule-A(JAMA),zonula occludens-1(ZO-1)and zinc finger protein A20(A20)in the jejunum,and HS also remarkably increased serum fluorescein isothiocyanate dextran(FITC-D),endotoxin and interleukin(IL)-1βcontents,serum diamine oxidase(DAO)and matrix metalloproteinase(MMP)-2 activities,nuclear factor kappa-B(NF-κB)p65 mRNA expression level,and protein expression levels of NF-κB p65 and MMP-2 in the jejunum.However,dietary supplementation with Zn,especially organic Zn as Zn-Prot M at 60 mg/kg,significantly decreased serum FITC-D,endotoxin and IL-1βcontents,serum DAO and MMP-2 activities,NF-κB p65 mRNA expression level,and protein expression levels of NF-κB p65 and MMP-2 in the jejunum of HS broilers,and notably promoted mRNA and protein expression levels of claudin-1,ZO-1 and A20.Conclusions Our results suggest that dietary Zn,especially 60 mg Zn/kg as Zn-Prot M,can alleviate HS-induced intestinal barrier function damage by promoting the expression of TJ proteins possibly via induction of A20-mediated suppression of the NF-κB p65/MMP-2 pathway in the jejunum of HS broilers. 展开更多
关键词 A20/NF-κB p65/MMP-2 pathway Broiler Heat stress Intestinal barrier function Tight junction proteins Zn
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Effects of glyphosate,antibiotics,and an anticoccidial drug on pancreatic gene expression and blood physiology in broilers 认领 引用
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作者 Georgi Yu.LAPTEV Daria G.TIURINA +13 位作者 Elena A.YILDIRIM Elena P.GORFUNKEL Larisa A.ILINA Valentina A.FILIPPOVA Andrei V.DUBROVIN Alisa S.DUBROVINA Evgeni A.BRAZHNIK Natalia I.NOVIKOVA Veronika K.MELIKIDI Kseniya A.SOKOLOVA Ekaterina S.PONOMAREVA Vasiliy A.ZAIKIN Darren K.GRIFFIN Michael N.ROMANOV 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2025年第2期185-199,共15页
Drugs and pesticide residues in broiler feed can compromise the therapeutic and production benefits of antibiotic(ANT)application and affect gene expression.In this study,we analyzed the expression of 13 key pancreati... Drugs and pesticide residues in broiler feed can compromise the therapeutic and production benefits of antibiotic(ANT)application and affect gene expression.In this study,we analyzed the expression of 13 key pancreatic genes and blood physiology parameters after administering one maximum residue limit of herbicide glyphosate(GLY),two ANTs,and one anticoccidial drug(AD).A total of 260 Ross 308 broilers aged 1-40 d were divided into the following four groups of 65 birds each:control group,which was fed the main diet(MD),and three experimental groups,which were fed MD supplemented with GLY,GLY+ANTs(enrofloxacin and colistin methanesulfonate),and GLY+AD(ammonium maduramicin),respectively.The results showed that the addition of GLY,GLY+ANTs,and GLY+AD caused significant changes in the expression of several genes of physiological and economic importance.In particular,genes related to inflammation and apoptosis(interleukin 6(IL6),prostaglandin-endoperoxide synthase 2(PTGS2),and caspase 6(CASP6))were downregulated by up to 99.1%,and those related to antioxidant protection(catalase(CAT),superoxide dismutase 1(SOD1)and peroxiredoxin 6(PRDX6))by up to 98.6%,compared to controls.There was also a significant decline in the values of immunological characteristics in the blood serum observed in the experimental groups,and certain changes in gene expression were concordant with changes in the functioning of the pancreas and blood.The changes revealed in gene expression and blood indices in response to GLY,ANTs,and AD provide insights into the possible mechanisms of action of these agents at the molecular level.Specifically,these changes may be indicative of physiological mechanisms to overcome the negative effects of GLY,GLY+ANTs,and GLY+AD in broilers. 展开更多
关键词 Glyphosate Antibiotic Anticoccidial drug Pancreas Broiler Gene expression Blood parameter
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In ovo sodium butyrate administration differentially impacts growth performance, intestinal barrier function, immune response, and gut microbiota characteristics in low and high hatch-weight broilers 认领 引用 被引量：3
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作者 Muhammad Zeeshan Akram Nadia Everaert Aleksandra Dunisławska 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第2期845-865,共21页
Background Hatch weight(HW)affects broiler growth and low HW(LHW)often leads to suboptimal performance.Sodium butyrate(SB)has been shown to promote growth through enhanced intestinal health.This study investi-gated ho... Background Hatch weight(HW)affects broiler growth and low HW(LHW)often leads to suboptimal performance.Sodium butyrate(SB)has been shown to promote growth through enhanced intestinal health.This study investi-gated how broilers with different HW responded to in ovo SB injection and whether SB could enhance gut health and performance in LHW chicks.Ross 308 broiler eggs were injected on incubation d 12 with physiological saline(control)or SB at 0.1%(SB1),0.3%(SB3),or 0.5%(SB5).Post-hatch,male chicks from each treatment were categorized as high HW(HHW)or LHW and assigned to 8 groups in a 4×2 factorial design.Production parameters were recorded periodically.Intestinal weight,length,and gene expression related to gut barrier function and immune response were examined on d 14 and 42.Cecal microbiota dynamics and predicted functionality were analyzed using 16S rRNA gene sequencing.Results SB treatments did not affect hatchability.HHW-control group exhibited consistently better weight gain and FCR than LHW-control group.SB dose-dependently influenced performance and gut health in both HW catego-ries,with greater effects in LHW broilers at 0.3%.LHW-SB3 group attained highest body weight on d 42,exceeding controls but not significantly differing from HHW-SB3 group.LHW-SB3 group showed upregulation of gut-barrier genes CLDN1 in ileum,TJP1 in jejunum and anti-inflammatory cytokine IL-10 in both jejunum and ileum on d 14.Addi-tionally,LHW-SB3 group upregulated mucin-producing MUC6 gene in ileum,while HHW-SB5 group increased pro-inflammatory IL-12p40 cytokine in caecum on d 42.LHW-SB3 group demonstrated shorter relative intestinal lengths,while HHW-SB5 had longer lengths.HHW-control group had higher bacterial diversity and growth-promoting bacte-ria while LHW-control group harbored the potential pathogen Helicobacter.SB reshaped gut microbiota biodiversity,composition,and predicted metabolic pathways in both HW categories.The LHW-SB3 group exhibited highest alpha diversity on d 14 and most beneficial bacteria at all timepoints.HHW-SB5 group presented increased pathogenic Escherichia-Shigella and Campylobacter on d 42.Conclusions HW significantly affects subsequent performance and SB has differential effects based on HW.LHW chicks benefited more from 0.3%SB,showing improvements in growth,intestinal development,health,and gut microbiota characteristics. 展开更多
关键词 Broiler production Flock uniformity Gut health In ovo stimulation Microbiome
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Unraveling the role of long non‑coding RNAs in chronic heat stress‑induced muscle injury in broilers 认领 引用
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作者 Zhen Liu Yingsen Liu +5 位作者 Tong Xing Jiaolong Li Lin Zhang Liang Zhao Yun Jiang Feng Gao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期206-217,共12页
Background Chronic heat stress(CHS)is a detrimental environmental stressor with a negative impact on the meat quality of broilers.However,the underlying mechanisms are not fully understood.This study investigates the ... Background Chronic heat stress(CHS)is a detrimental environmental stressor with a negative impact on the meat quality of broilers.However,the underlying mechanisms are not fully understood.This study investigates the effects of CHS on long non-coding RNA(lncRNA)expression and muscle injury in broilers,with a focus on its implications for meat quality.Results The results showed that CHS diminished breast muscle yield,elevated abdominal fat deposition,induced cellular apoptosis(P<0.05),and caused myofibrosis.Transcriptomic analysis revealed 151 differentially expressed(DE)lncRNAs when comparing the normal control(NC)and HS groups,214 DE lncRNAs when comparing the HS and PF groups,and 79 DE lncRNAs when comparing the NC and pair-fed(PF)groups.After eliminating the confounding effect of feed intake,68 lncRNAs were identified,primarily associated with cellular growth and death,signal transduc-tion,and metabolic regulation.Notably,the apoptosis-related pathway P53,lysosomes,and the fibrosis-related gene TGF-β2 were significantly upregulated by lncRNAs.Conclusions These findings indicate that chronic heat stress induces cellular apoptosis and muscle injury through lncRNA,leading to connective tissue accumulation,which likely contributes to reduced breast muscle yield and meat quality in broilers. 展开更多
关键词 Apoptosis Broiler Chronic heat stress LncRNA Muscle
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Dietary processed former foodstuffs for broilers:impacts on growth performance,digestibility,hematobiochemical profiles and liver gene abundance 认领 引用
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作者 Karthika Srikanthithasan Marta Gariglio +14 位作者 Elena Diaz Vicuna Edoardo Fiorilla Barbara Miniscalco Valeria Zambotto Eleonora Erika Cappone Nadia Stoppani Dominga Soglia Federica Raspa Joana Nery Andrea Giorgino Roser Sala Andrés Luis Martínez Marínz Josefa Madrid Sanchez Achille Schiavone Claudio Forte 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2025年第1期364-376,共13页
Background The present experiment aimed to evaluate the effects of commercially processed former foodstuffs(cFF)as dietary substitutes of corn,soybean meal and soybean oil on the growth performance,apparent total trac... Background The present experiment aimed to evaluate the effects of commercially processed former foodstuffs(cFF)as dietary substitutes of corn,soybean meal and soybean oil on the growth performance,apparent total tract digestibil-ity(ATTD),hematobiochemical profiles,and liver gene abundance in broiler chickens.Two hundred one-day-old male ROSS-308 chicks were assigned to 4 dietary groups(5 replicates of ten birds per replicate)according to their average body weight(BW,38.0±0.11 g).All groups received a two-phase feeding program:starter,d 1–12 and grower,d 12–33.The control group(cFF0)was fed a standard commercial feed based on corn,soybean meal and soybean oil.The other three groups received diets in which the feed based on corn,soybean meal,and soybean oil was partially replaced with cFF at a substitution level of 6.25%(cFF6.25),12.5%(cFF12.5)or 25%(cFF25)for the following 33 d.Results The growth performance data showed no differences in BW or average daily gain among groups,although the average daily feed intake decreased during the grower period(12–33 d)and over entire experimental period(1–33 d)in a linear manner as the cFF inclusion level rose(P=0.026),positively affecting the gain to feed ratio(P=0.001).The ATTD of dry matter of the cFF-fed groups were greater with respect to control group and increased throughout the experimental period,whereas the ATTD of ether extract linearly decreased with increasing levels of cFF-fed groups compared with control group and throughout the experimental period(P<0.05).Additionally,a lin-ear increase in the heterophil to lymphocyte ratio,serum cholesterol,triglycerides and alanine-aminotransferase were observed with increasing dietary levels of cFF(P<0.05);however,no differences were observed in lipoprotein lipase or sterol regulatory element binding transcription factor gene abundance.Conclusions The results of this experiment demonstrate that it is possible to incorporate cFF into nutritionally balanced diets for broiler chickens,even up to 25%substitution levels,for up to 33 d without adversely impacting the overall growth performance of male broiler chickens raised under commercial conditions.Further studies are essential to validate the hematological trait findings. 展开更多
关键词 Alternative feed Broiler chicken Digestibility Former foodstuff Gene abundance Growth performance
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Effects of dietary graded levels of cinnamon essential oil and its combination with bamboo leaf flavonoid on immune function, antioxidative ability and intestinal microbiota of broilers 认领 引用 被引量：21
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作者 YANG Yun-feng ZHAO Lu-lu +4 位作者 SHAO Yu-xin LIAO Xiu-dong ZHANG Li-yang LU Lin LUO Xu-gang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第9期2123-2132,共10页
Cinnamon essential oil(CEO)and its combination with bamboo leaf flavonoid(BLF)have been shown to exhibit an additive antibacterial effect in vitro,but their functions in broilers were not clear.An experiment was condu... Cinnamon essential oil(CEO)and its combination with bamboo leaf flavonoid(BLF)have been shown to exhibit an additive antibacterial effect in vitro,but their functions in broilers were not clear.An experiment was conducted to investigate the effects of dietary graded levels of CEO and its combination with BLF on the growth performance,immune responses,antioxidative ability,and intestinal morphology and microbiota of broilers fed a corn-soybean meal basal diet.A total of 576 1-d-old Arbor Acres commercial male broilers were randomly allotted to 9 treatments with 8 replicates of 8 birds each in a completely randomized design.Birds were fed on a basal corn-soybean meal diet(control,without plant extracts and antibiotics),or the basal diet supplemented with 50 mg of aureomycin kg^-1,50,100,200,400,or 800 mg of CEO kg^-1,a combination of 100 mg of CEO and 16.7 mg of BLF kg^-1,or a combination of 200 mg of CEO kg^-1 and 33.3 mg of BLF kg^-1 for 42 d.Dietary treatment affected(P0.16)on all other measured indices.The addition of 400 mg of CEO kg^-1 or a combination of 200 mg CEO kg^-1 and 33.3 mg BLF kg^-1 increased(P<0.02)serum IgM contents on d 42.Dietary supplementation with 100 or 200 mg CEO kg^-1,or 50 mg aureomycin kg^-1 decreased(P<0.003)liver MDA contents on d 21.In addition,the supplement of 100 mg CEO kg^-1 increased(P<0.002)the Lactobacillus relative abundance in caecum on d 21 and Bifidobacterium relative abundance in caecum on d 42,and decreased(P<0.0001)E.coli relative abundance in caecum on d 42.The results indicated that dietary supplementation with CEO,an alternative to aureomycin,improved the immune status,antioxidantative ability and cecal microbiota of broilers,and dietary supplementation with the combinations of CEO and BLF did not exhibit further effects.Dietary supplementation with 100 mg CEO kg^-1 is beneficial for broilers fed a corn-soybean meal basal diet. 展开更多
关键词 plant extracts immune responses antioxidative ability intestinal morphology cecal microbiota broilers
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Long-term effects of oral tea polyphenols and Lactobacillus brevis M8 on biochemical parameters, digestive enzymes, and cytokines expression in broilers 认领 引用 被引量：13
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作者 Hua-li LI Zong-jun LI +4 位作者 Zhong-shan WEI Ting LIU Xiao-zuo ZOU Yong LIAO Yu LUO 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2015年第12期1019-1026,共8页
This study investigates the long-term effects of oral tea polyphenols （TPs） and Lactobacillus brevis M8 （LB） on biochemical parameters, digestive enzymes, and cytokines expression in broilers. In experiment 1,240 ... This study investigates the long-term effects of oral tea polyphenols （TPs） and Lactobacillus brevis M8 （LB） on biochemical parameters, digestive enzymes, and cytokines expression in broilers. In experiment 1,240 broiler chickens were selected to investigate the effects of 0.06 g/kg body weight （BW） TP and 1.0 ml/kg BW LB on broilers; in experiment 2, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of TP （0.03, 0.06, and 0.09 g/kg BW） combined with 1.0 ml/kg BW LB on broilers; in experiment 3, 180 broiler chickens were assigned randomly to three groups to investigate the effects of different dosages of LB （0.5, 1.0, and 1.5 ml/kg BW） combined with 0.06 g/kg BW TP on broilers. The results showed that TP and LB affected serum biochemical parameters, and TP reduced serum cholesterol （CHO） and low-density lipoprotein cholesterol （LDL-C） abundances in a dosage-dependent manner （P〈0.05） on Day 84. Meanwhile, broilers fed a diet supplemented with TP or LB had a lower intestinal lipase activity on Day 84 compared with the control group （P〈0.05）. Middle and high dosages of TP increased pancreatic lipase and proventriculus pepsin activities （P〈0.05）. Also middle and high dosages of LB significantly enhanced pancreatic lipase activity （P〈0.05）, while high LB supplementation inhibited intestinal trypsase （P〈0.05） on Day 84. Furthermore, both TP and LB reduced intestinal cytokine expression and nuclear factor-K B （NF-KB） mRNA level on Days 56 and 84. In conclusion, long-term treatment of TP and LB improved lipid metabolism and digestive enzymes activities, and affected intestinal inflammatory status, which may be associated with the NF-KB signal. 展开更多
关键词 Tea polyphenols Lactobacillus brevis Cytokines Broilers
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Effects of Enteromorpha prolifera polysaccharides on growth performance,intestinal barrier function and cecal microbiota in yellow-feathered broilers under heat stress 认领 引用 被引量：9
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作者 Wenchao Liu Huimei Liu +3 位作者 Yaoyao Wang Zhongxiang Zhao Balamuralikrishnan Balasubramanian Rajesh Jha 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第1期344-361,共18页
Background Global warming leading to heat stress(HS)is becoming a major challenge for broiler production.This study aimed to explore the protective effects of seaweed(Enteromorpha prolifera)polysaccharides(EPS)on the ... Background Global warming leading to heat stress(HS)is becoming a major challenge for broiler production.This study aimed to explore the protective effects of seaweed(Enteromorpha prolifera)polysaccharides(EPS)on the intestinal barrier function,microbial ecology,and performance of broilers under HS.A total of 144 yellow-feathered broilers(male,56 days old)with 682.59±7.38 g were randomly assigned to 3 groups:1)TN(thermal neutral zone,23.6±1.8℃),2)HS(heat stress,33.2±1.5℃ for 10 h/d),and 3)HSE(HS+0.1%EPS).Each group contained 6 replicates with 8 broilers per replicate.The study was conducted for 4 weeks;feed intake and body weights were measured at the end of weeks 2 and 4.At the end of the feeding trial,small intestine samples were collected for histomorphology,antioxidant,secretory immunoglobulin A(s Ig A)content,apoptosis,gene and protein expression analysis;cecal contents were also collected for microbiota analysis based on 16S r DNA sequencing.Results Dietary EPS promoted the average daily gain(ADG)of broilers during 3–4 weeks of HS(P<0.05).At the end of HS on broilers,the activity of total superoxide dismutase(T-SOD),glutathione S-transferase(GST),and the content of s Ig A in jejunum were improved by EPS supplementation(P<0.05).Besides,dietary EPS reduced the epithelial cell apoptosis of jejunum and ileum in heat-stressed broilers(P<0.05).Addition of EPS in HS group broilers'diet upregulated the relative m RNA expression of Occludin,ZO-1,γ-GCLc and IL-10 of the jejunum(P<0.05),whereas downregulated the relative m RNA expression of NF-κB p65,TNF-αand IL-1βof the jejunum(P<0.05).Dietary EPS increased the protein expression of Occludin and ZO-1,whereas it reduced the protein expression of NF-κB p65 and MLCK(P<0.01)and tended to decrease the protein expression of TNF-α(P=0.094)in heat-stressed broilers.Furthermore,the proportions of Bacteroides and Oscillospira among the three groups were positively associated with jejunal apoptosis and pro-inflammatory cytokine expression(P<0.05)and negatively correlated with jejunal Occludin level(P<0.05).However,the proportions of Lactobacillus,Barnesiella,Subdoligranulum,Megasphaera,Collinsella,and Blautia among the three groups were positively related to ADG(P<0.05).Conclusions EPS can be used as a feed additive in yellow-feathered broilers.It effectively improves growth performance and alleviates HS-induced intestinal injury by relieving inflammatory damage and improving the tight junction proteins expression.These beneficial effects may be related to inhibiting NF-κB/MLCK signaling pathway activation and regulation of cecal microbiota. 展开更多
关键词 Broilers Cecal microbiota Heat stress Intestinal barrier function Seaweed polysaccharides
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Effects of in ovo delivered xylo-and mannan-oligosaccharides on growth performance,intestinal immunity,cecal short-chain fatty acids,and cecal microbiota of broilers 认领 引用 被引量：4
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作者 Amit Kumar Singh Utsav Prakash Tiwari +1 位作者 Birendra Mishra Rajesh Jha 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2022年第4期1099-1114,共16页
Background:This study investigated a novel in ovo feeding strategy to determine the prebiotic effects of xylo-and mannan-oligosaccharides(XOS and MOS)differing in the degree of polymerization.A total of 192 fertilized... Background:This study investigated a novel in ovo feeding strategy to determine the prebiotic effects of xylo-and mannan-oligosaccharides(XOS and MOS)differing in the degree of polymerization.A total of 192 fertilized eggs were divided into 6 treatment groups:i)normal saline control(NSC),ii)xylotriose(XOS3),iii)xylotetraose(XOS4),iv)mannotriose(MOS3),v)mannotetraose(MOS4),and vi)no injection control(NIC),each containing 4 replicate trays with 8 eggs per replicate.On d 17 of incubation,3 mg of oligosaccharides(except for controls)dissolved in 0.5 mL of 0.85%normal saline were injected into the amnion of Cobb 500 broilers eggs.After hatch,the chicks were raised for 28 d under standard husbandry practices and were fed a commercial broilers diet ad libitum,and samples were collected periodically.Results:The hatchability,growth performance,and relative weights of breast,drumstick,liver,and proventriculus were not different among the treatments(P>0.05).The XOS3 injection increased the total short-chain fatty acid production at d 28 compared with both control groups(P0.05).On the hatch day,the expression level of the CD3 gene(a T cell marker)was increased by XOS3,while the IL-10 gene(a marker of anti-inflammatory cytokine)was reduced by MOS4(P0.05),except XOS4 increased IL-4 compared with NSC(P<0.05).The broilers in the MOS4 group had higher operational taxonomic units(OTUs)and had more differentially abundant taxa,including order Lactobacillales and family Leuconostocaceae(P<0.05)than both controls on d 28.The predictive functional profiling indicated that the linoleic acid metabolism pathway was enriched in the cecal microbiota of the XOS3 group compared with both controls(P<0.05).Conclusion:The effects of these XOS and MOS on ileal mucosa and immunity are transient,but the effects on fermentation and cecal microbiota are prolonged,and further research is warranted to determine their use as a gut health promoter in poultry. 展开更多
关键词 Broilers Gut health Immunity in ovo Mannanoligosaccharides Microbiota Oligosaccharides Prebiotics Xylooligosaccharides
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Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions 认领 引用 被引量：4
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作者 Fan Zhang Jiantao Yang +5 位作者 Qinyi Zhan Hao Shi Yanhe Li Dinggang Li Yingge Li Xiaojun Yang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第5期2079-2092,共14页
Background Intestinal health plays a pivotal role in broiler chicken growth.Oregano aqueous extract(OAE)effec-tively exerts anti-inflammatory and antibacterial effects.However,the protective effects of OAE on intestin... Background Intestinal health plays a pivotal role in broiler chicken growth.Oregano aqueous extract(OAE)effec-tively exerts anti-inflammatory and antibacterial effects.However,the protective effects of OAE on intestinal health in broilers and the underlying mechanism remain unclear.This study aimed to investigate the potential effects of OAE on growth performance,the gut microbiota and intestinal health.A total of 8401-d-old male and female broilers(Arbor Acres)were randomly allocated into 6 groups as follows:basal diet(Con),Con+antibiotics(Anti,colistin sulfate 7 g/kg,roxarsone 35 g/kg),Con+400,500,600 and 700 mg/kg OAE(OAE400,OAE500,OAE600 and OAE700).Subse-quently,fermentation in vitro together with oral administration trials were carried out to further assess the function of OAE on intestinal health of broilers.Results Dietary 700 mg/kg OAE supplementation resulted in an increase(P<0.05)in body weight and a decrease(P<0.05)in feed conversion ratio when compared with the control during d 22 to 42 of the trial.OAE addition resulted in lower(P<0.05)jejunal crypt depth and mRNA expression of IL-4 and IL-10 at d 42.In addition,dietary OAE addition increased the abundance of Firmicutes(P=0.087)and Lactobacillus(P<0.05)in the cecum,and increased(P<0.05)the content of acetic acid and butyric acid.In the in vitro fermentation test,OAE significantly increased(P<0.05)the abundance of Lactobacillus,decreased(P<0.05)the abundance of unspecified_Enterobacteriaceae,and increased the content of acetic acid(P<0.05).In the oral administration trial,higher(P<0.05)IL-4 expression was found in broilers when oral inoculation with oregano fermentation microorganisms at d 42.And SIgA content in the ileum was significantly increased(P=0.073)when giving OAE fermentation supernatant.Conclusions Dietary OAE addition could maintain intestinal health and improve growth performance through enhancing intestinal mucosal immunity and barrier function mediated by gut microbiota changes. 展开更多
关键词 Broilers Gut microbiota Intestinal health Oregano aqueous extract
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Nonphytate Phosphorus Requirement and Efficacy of a Genetically Engineered Yeast Phytase for Yellow Broilers at 22-to 42-d-Old Age 认领 引用 被引量：6
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作者 JIANG Zong-yong ZHOU Gui-lian +4 位作者 LIN Ying-cai JIANG Shou-qun LI De-zhong XI Peng-bin ZHANG Han-xing 《Agricultural Sciences in China》 CSCD 2011年第2期304-311,共8页
An experiment was conducted to investigate the requirement of nonphytate phosphorus(nPP)and efficacy of a genetically engineered yeast phytase(PHY A)for Lingnan yellow broilers from 22-to 42-d-old age.A total of 13201... An experiment was conducted to investigate the requirement of nonphytate phosphorus(nPP)and efficacy of a genetically engineered yeast phytase(PHY A)for Lingnan yellow broilers from 22-to 42-d-old age.A total of 13201-d-old male chicks were randomly divided into 11 dietary treatment groups,which consisted of 4 replicate floor pens with 30 birds per pen.The control group(treatment 1)was fed with basal diet of nPP 0.08%without dicalcium phosphate or phytase supplementation.Dietary levels of nPP were 0.16,0.24,0.32,0.40,0.48,and 0.56%,respectively,for treatments 2 to 7,through addition of dicalcium phosphate(chemistry grade)to the basal diet.Diets of treatments 8 to 11 were supplemented with PHY A at 200,400 and 600 U kg-1,a commercial phytase product(PHY B)at 400 U kg-1 level,respectively.The birds in 0.32-0.56%nPP groups gained more than those of the other groups(P0.05).The nPP supplementation significantly improved feed intake(P0.05).The feed gain ratio was significantly decreased by 0.40%nPP diet compared to the control birds(P0.05).The level of 0.48%nPP was required for optimum tibia development.The additions of PHY A at 400 and 600 U kg-1 level and PHY B all significantly improved ADG(P0.05),ADFI(P0.05),and dry defatted tibia weight(P0.05).Similarly,the percentage of tibia ash was increased by 600 U kg-1 PHY A supplementation(P0.05).The requirement of nPP for maximal ADG and highest percentage tibia ash both was 0.40%.The phosphorus equivalency value of PHY A was estimated as 685 U kg-1 for male yellow broilers of 22-to 42-d-old age. 展开更多
关键词 nonphytate phosphorus growth tibia ash genetically engineered yeast phytase phosphorus equivalency value yellow broilers
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Effects of Dietary Isoflavone Supplementation on Meat Quality and Oxidative Stability During Storage in Lingnan Yellow Broilers 认领 引用 被引量：4
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作者 JIANG Shou-qun JIANG Zong-yong +2 位作者 ZHOU Gui-lian LIN Ying-cai ZHENG Chun-tian 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第2期387-393,共7页
To investigate the effects of a synthetic isoflavone （SI） on meat quality and oxidative stability, 1 500 43-d-old Lingnan yellow male broilers were randomly assigned to five dietary treatments： diets supplemented w... To investigate the effects of a synthetic isoflavone （SI） on meat quality and oxidative stability, 1 500 43-d-old Lingnan yellow male broilers were randomly assigned to five dietary treatments： diets supplemented with 0, 10, 20, 40, or 80 mg SI kg-1, fed ad libitum for a period of 3 wk. After refrigerated storage, a＊ value of the meat increased with dietary SI supplementation using 10 and 40 mg kg-1 level （P〈0.05）, L＊ value decreased （P〈0.05）, and 40 mg kg-j increased the pH （P〈0.05）. Supplementation with SI, at all levels, increased water holding capacity （P〈0.05） and decreased lactic acid content of meat （P〈0.05）. The concentration of malondialdehyde at 72 h decreased linearly （P=0.005） and quadratically （P=0.004） with increasing levels of SI. Dietary SI at 20 and 40 mg kg-1 levels enhanced total superoxide dismutase activity in meat （P〈0.05）. Meat pH quadratically decreased as the storage time increased （P〈0.05）, with the highest value at 24 h （P〈0.05）. Lactic acid and malondialdehyde concentrations of meat increased over time, with value at 96 h being far higher than at earlier times （P〈0.05）. Supplemental SI linearly and quadratically increased the mRNA abundance ofglutathione peroxidase （GPX） （P=0.001 and P=0.002） and catalase （CAT） （P=0.003 and P=0.006） in breast muscle. The results from this study indicate that dietary supplementation with SI can improve meat quality during refrigerated storage by decreasing lipid peroxidation and enhancing oxidative stability and, for male broilers from 43 to 63 d of age, the optimal level of SI was 40 mg kg-1. 展开更多
关键词 Lingnan yellow broilers isoflavone meat quality oxidative stability glutathione peroxidase mRNA
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