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Molecular Characterization of a Dehydroascorbate Reductase from Pinus bungeana 认领 引用 被引量：5
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作者 Hai-Ling Yang Ying-Ru Zhao +3 位作者 Cai-Ling Wang Zhi-Ling Yang Qing-Yin Zeng Hai Lu 《Journal of Integrative Plant Biology》 SCIE CAS 2009年第11期993-1001,共9页
Dehydroascorbate reductase （DHAR） plays a critical role in the ascorbate-glutathione recycling reaction for most higher plants. To date, studies on DHAR in higher plants have focused largely on Arabidopsis and agric... Dehydroascorbate reductase （DHAR） plays a critical role in the ascorbate-glutathione recycling reaction for most higher plants. To date, studies on DHAR in higher plants have focused largely on Arabidopsis and agricultural plants, and there is virtually no information on the molecular characteristics of DHAR in gymnosperms. The present study reports the cloning and characteristics of a DHAR （PbDHAR） from a pine, Pinus bungeana Zucc. ex Endl. The PbDHAR gene encodes a protein of 215 amino acid residues with a calculated molecular mass of 24.26 kDa. The predicted 3-D structure of PbDHAR showed a typical glutathione S-transferase fold. Reverse transcripUon-polymerase chain reaction revealed that the PbDHAR was a constitutive expression gene in P. bungeana. The expression level of PbDHAR mRNA in P. bungeana seedlings did not show significant change under high temperature stress. The recombinant PbDHAR was overexpressed in Escherichia coil following purification with affinity chromatography. The recombinant PbDHAR exhibited enzymatic activity （19.84 i.mnol/min per mg） and high affinity （a Krn of 0.08 mM） towards the substrates dehydroascorbate （DHA）. Moreover, the recombinant PbDHAR was a thermostable enzyme, and retained 77% of its initial activity at 55℃. The present study is the first to provide a detailed molecular characterization of the DHAR in P. bungeana. 展开更多
关键词 cloning dehydroascorbate reductase enzyme activity gymnosperm.
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Role of endogenous wheat enzymes on gluten cross-linking and dough and bread properties:a review 认领 引用
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作者 Ying Liang Chunlei Zheng +3 位作者 Yaping Zhang Hao Liu Penghui Zhang Jinshui Wang 《Food Innovation and Advances》 2025年第3期321-333,共13页
The quality of wheat-based products is significantly influenced by the structure and properties of gluten,and these are affected by various endogenous enzymes.However,the specific mechanisms of these enzymes,such as s... The quality of wheat-based products is significantly influenced by the structure and properties of gluten,and these are affected by various endogenous enzymes.However,the specific mechanisms of these enzymes,such as sulfhydryl oxidase,protein disulfide isomerase,ascorbate oxidase,and dehydroascorbate reductase have not been well studied.The role of these enzymes in enhancing gluten network formation and dough properties is not yet fully understood.This review examines the types,structures,and mechanisms of these key enzymes,with a focus on their roles in promoting disulfide bond formation and improving dough rheology and bread quality.By elucidating the mechanisms through which these enzymes directly or indirectly influence the structure,function,and physicochemical properties of gluten proteins,this review provides critical insights that advance understanding of gluten crosslinking and lay the groundwork for practical strategies to enhance the quality and safety of wheat-based products in food processing. 展开更多
关键词 endogenous enzymes sulfhydryl oxidase ascorbate oxidase endogenous enzymeshoweverthe protein disulfide isomerase sulfhydryl oxidaseprotein disulfide isomeraseascorbate oxidaseand dehydroascorbate reductase enhancing gluten
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青花菜雄性不育相关基因BoDHAR的克隆与表达分析 认领 引用 被引量：8
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作者 张国裕 康俊根 +2 位作者 张延国 程智慧 王晓武 《生物工程学报》 CAS 北大核心 2006年第5期751-756,共6页
以一个与甘蓝显性核不育相关的差异表达片段的序列为信息探针,通过在NCBI与TAIR网站数据库中进行同源EST序列搜索,经人工拼接、RT-PCR、PCR克隆与序列分析,获得了青花菜脱氢抗坏血酸还原酶DHARdehydroascorbatereductase基因的cDNA与DN... 以一个与甘蓝显性核不育相关的差异表达片段的序列为信息探针,通过在NCBI与TAIR网站数据库中进行同源EST序列搜索,经人工拼接、RT-PCR、PCR克隆与序列分析,获得了青花菜脱氢抗坏血酸还原酶DHARdehydroascorbatereductase基因的cDNA与DNA全长序列,命名为BoDHAR。并利用双链接头介导PCR的染色体步行技术(genomewalking)克隆了其上游644bp的5′端序列。所获的BoDHAR基因全长1486bp,存在两个内含子,DNA编码区序列633bp,编码210个氨基酸;序列分析表明BoDHAR与同源基因AT1G19570.1cDNA序列有82.3%的一致性,推导的氨基酸序列有79.6%的一致性;编码的水溶性蛋白存在多个磷酸化位点;5′端上游区存在明显的转录调控序列。半定量RT-PCR结果表明BoDHAR在可育系花蕾中的表达量明显高于不育系花蕾,在花药中的表达明显高于其它部位。 展开更多
关键词 青花菜(Brassica oleracea L. var. italica DHAR(dehydroascorbate reductase) 基因克隆 RT-PCR
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GLUT1-mediated effective anti-miRNA21 pompon for cancer therapy 认领 引用 被引量：8
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作者 Qin Guo Chao Li +8 位作者 Wenxi Zhou Xinli Chen Yu Zhang Yifei Lu Yujie Zhang Qinjun Chen Donghui Liang Tao Sun Chen Jiang 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2019年第4期832-842,共11页
Oncogenic microRNAs are essential components in regulating the gene expression of cancer cells. Especially miR21, which is a major player involved of tumor initiation, progression, invasion and metastasis in several c... Oncogenic microRNAs are essential components in regulating the gene expression of cancer cells. Especially miR21, which is a major player involved of tumor initiation, progression, invasion and metastasis in several cancers. The delivery of anti-miR21 sequences has significant potential for cancer treatment. Nevertheless, since anti-miR21 sequences are extremely unstable and they need to obtain certain concentration to function, it is intensely difficult to build an effective delivery system for them.The purpose of this work is to construct a self-assembled glutathione(GSH)-responsive system with tumor accumulation capacity for effective anti-miR21 delivery and cancer therapy. A novel drug delivery nanosphere carrying millions of anti-miR21 sequences was developed through the rolling circle transcription(RCT) method. GSH-responsive cationic polymer polyethyleneimine(pOEI) was synthesized to protect the nanosphere from degradation by Dicer or other RNase in normal cells and optimize the pompon-like nanoparticle to suitable size. Dehydroascorbic acid(DHA), a targeting molecule, which is a substrate of glucose transporter 1(GLUT 1) and highly expressed on malignant tumor cells, was connected to pOEI through PEG, and then the polymer was used for contracting a RNA nanospheres into nanopompons. The anti-miR21 nanopompons showed its potential for effective cancer therapy. 展开更多
关键词 MiRNA21 Dehydroascorbic acid Rolling circle transcription Self-assembly RNAi nanoparticle Tumor-targeting Nanomedicine Cancer treatment
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