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Bioaccumulation and function analysis of glutathione S-transferase isoforms in Manila clam Ruditapes philippinarum exposed to different kinds of PAHs 认领 引用
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作者 Yueyao Zhou Linlin Yao +1 位作者 Luqing Pan Hongdan Wang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2022年第2期129-139,共11页
This study analyzed the function of different glutathione S-transferase(GST)isoforms and detoxification metabolism responses in Manila clam,Ruditapes philippinarum,exposed to 4 kinds of polycyclic aromatic hydrocarbon... This study analyzed the function of different glutathione S-transferase(GST)isoforms and detoxification metabolism responses in Manila clam,Ruditapes philippinarum,exposed to 4 kinds of polycyclic aromatic hydrocarbons(PAHs)single,and their mixtures for 15 days under laboratory conditions.13 kinds of GSTs in R.philippinarum were classified,and the results of tissue distribution indicated that 12 kinds of GSTs(except GST sigma 3)expressed most in digestive glands.We detected the m RNA expression levels of aryl hydrocarbon receptor signaling pathway,and detoxification system in digestive glands of clams exposed to benzo[a]pyrene(Ba P),chrysene(CHR),benzo[a]anthracene(Ba A),benzo[b]fluoranthene(Bb F),and Ba P+CHR+Ba A+Bb F,respectively.Among these genes,we selected GST-sigma,GST-omega and GST-pi as potential indicators to Ba P;GST-sigma,GST-A and GST-rho to CHR;GST-pi,GST-sigma,GST-A,GST-rho and GST-microsomal to Ba A;GST-theta and GST-mu to Bb F;while GST-pi and GST-mu to the mixture of Ba P,CHR,Ba A and Bb F.Additionally,the bioaccumulation of PAHs in tissues increased remarkably over time,and showed an obvious doseeffect.Under the same concentration,the bioaccumulation in single exposure group was higher than that in mixture group,and the bioaccumulation of PAHs in tissues with different concentrations of stress was irregular.The results revealed the metabolic differences and bioaccumulation rules in clams exposed to four kinds of PAHs,and provided more valuable information for the PAHs risk assessment. 展开更多
关键词 Ruditapes philippinarum PAHs bioaccumulation Glutathione S-transferase function analysis
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Function Analysis of Urban Rail Transit Smart Station 认领 引用 被引量：1
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作者 Can Chen 《Journal of Architectural Research and Development》 2023年第4期26-31,共6页
In order to make rail transit smart stations serve passengers better,the potential of smart stations should be optimized to reduce time cost,and relieve traffic congestion.In this paper,the construction of smart stati... In order to make rail transit smart stations serve passengers better,the potential of smart stations should be optimized to reduce time cost,and relieve traffic congestion.In this paper,the construction of smart stations based on the management experience of a subway station and the benefits of smart stations were analyzed and discussed.The construction of smart stations,as well as the key technologies for the construction of smart stations,will aid in the automation and intelligent management of subway stations. 展开更多
关键词 Urban rail transit Smart station Function analysis
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Two adult-onset cases of CSF1R gene mutation-related leukoencephalopathy and gain-offunction analysis of the gene mutations 认领 引用
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作者 HUANG Zhengping 《China Medical Abstracts(Internal Medicine)》 2026年第2期126-126,共1页
Objective To investigate the clinical characteristics of 2 cases with adult-onset leukoencephalopathy with axonal spheroids and pigmented glia(ALSP),analyze the genetic features of colony-stimulating factor 1 receptor... Objective To investigate the clinical characteristics of 2 cases with adult-onset leukoencephalopathy with axonal spheroids and pigmented glia(ALSP),analyze the genetic features of colony-stimulating factor 1 receptor(CSFlR)gene mutations in patients,and to perform functional validation.Methods Clinical and imaging data from 2 ALSPpatients diagnosed at the Second Affiliated Hospital of Fujian Medical University between 2020 and 2021 were collected.Whole-exome sequencing was used to identify potential pathogenic mutations.The function of mutated genes was validated through protein function prediction software and in vitro assays. 展开更多
关键词 identify potential pathogen adult onset leukoencephalopathy investigate clinical characteristics clinical imaging data functional validationmethods CSF R gene mutation axonal spheroids gain function analysis
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Sparse Canonical Correlation Analysis with L2,1-Norm for Functional Data 认领 引用
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作者 Zhang Zejiang Yang Zhixia +1 位作者 Ye Junyou Wang Yulan 《新疆大学学报（自然科学版中英文）》 CAS 2026年第3期305-323,共19页
Functional canonical correlation analysis is a key method in multivariate statistics for identifying optimal linear correlations between two functional datasets.However,some functions within these datasets may exhibit... Functional canonical correlation analysis is a key method in multivariate statistics for identifying optimal linear correlations between two functional datasets.However,some functions within these datasets may exhibit anomalies such as sudden changes or fluctuations that deviate from the overall trend,resulting to inaccurate results.To address this,we propose an improved method:Sparse functional canonical correlation analysis based on the L2,1-norm.This approach reduces outliers by optimizing the selection of orthogonal basis functions,thereby enhancing the accuracy and reliability of the analysis.Numerical experiments show that the L2,1-norm-based method significantly outperforms traditional methods. 展开更多
关键词 functional canonical correlation analysis L2,1-norm functional data outliers orthogonal basis function
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Discriminant Function Analysis for Sex Determination Using Lower Limb Anthropometric Variables in Young Adults of Nigeria Population 认领 引用
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作者 Choy Ker Woon Aliyu Jaafar Abdulhamid Usman 《Journal of Forensic Science and Medicine》 2025年第2期125-130,共6页
Background:Sex determination is a fundamental aspect of forensic anthropology.Anthropometric analyses,particularly of the lower limb,have emerged as valuable tools,offering insights into population-specific for sex cl... Background:Sex determination is a fundamental aspect of forensic anthropology.Anthropometric analyses,particularly of the lower limb,have emerged as valuable tools,offering insights into population-specific for sex classification.While numerous studies have investigated sex dimorphism utilizing various morphometric parameters,limited attention has been directed toward lower limb anthropometry in Nigerian populations.Objective:The objective is to investigate the lower limb anthropometric variables for sex determination among young adults in the Nigerian population.Methodology:A cross-sectional study was conducted with a total of 360 young adults(180 males and 180 females)aged 17-34 years old by random sampling.The anthropometric variables such as femur length,tibia length,foot length,foot width,and foot index were measured.Correlation and discriminant function analysis were performed to ascertain the most discriminative variables for sex classification.Results:The femoral height,knee height,foot length,and foot width among males scored higher mean values than females.Strong positive correlation is seen between femoral height and knee height in males(r=0.851,P<0.05)and females(r=0.781,P<0.05).Foot length shows the highest potential in discriminating sex with percentage accuracy of 72.8%after cross-validation in univariate analysis.However,following multivariate discriminant function analysis,age,knee height,foot length,and foot width were chosen as the significant variables and increased percentage accuracy to 78%after cross-validation.Conclusion:Femoral height,knee height,foot length,and foot width are anthropometric parameters suitable for assessing sexual dimorphism among young adults in the Nigerian population. 展开更多
关键词 Anthropometric discriminant function analysis lower limb Nigeria population sexual dimorphism young adult
Genome-wide identification of SmJAZ gene family in eggplant and functional mechanism analysis of SmJAZ9 modulating high temperature and darkness stress-regulated anthocyanin biosynthesis 认领 引用 被引量：2
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作者 Yue Wang Hong Wang +5 位作者 Yu Xin Mingyue Yuan Tuo Ji Duanyin Gu Fengjuan Yang Jing Li 《Vegetable Research》 2025年第1期314-323,共10页
The biosynthesis of anthocyanin in plants is regulated by temperature,light,jasmonic acid(JA),among others.JASMONATE ZIM-DOMAIN(JAZ)proteins are critical repressors of the jasmonic acid(JA)pathway,the role of JAZ prot... The biosynthesis of anthocyanin in plants is regulated by temperature,light,jasmonic acid(JA),among others.JASMONATE ZIM-DOMAIN(JAZ)proteins are critical repressors of the jasmonic acid(JA)pathway,the role of JAZ proteins on anthocyanin biosynthesis under high-temperature or dark conditions remains unexplored.Here,we identified 16 SmJAZ genes in eggplant and characterized their evolutionary relationships,gene structures,and hightemperature(HT)and darkness stress-responsive expression patterns.Both the HT and darkness treatments significantly inhibit anthocyanin biosynthesis in eggplant peel.Correlation analysis revealed that the SmJAZ9 expression strongly correlated with anthocyanin biosynthesis-related genes under HT and darkness conditions.Transient expression assay in eggplant peels demonstrated that nuclear-localized SmJAZ9 is a positive regulator,enhancing anthocyanin biosynthesis via interaction with the transcription factor SmMYB113,thereby promoting SmCHS and SmDFR transcription.Additionally,SmJAZ9 interacted with SmMYC2 but not SmCOI1,suggesting a JA signaling-independent regulatory mechanism under HT and darkness conditions.This study revealed a novel JAZ-mediated regulatory module integrating JA signaling,environmental stress responses,and anthocyanin biosynthesis,providing potential targets for improving eggplant fruit quality. 展开更多
关键词 smjaz genes functional mechanism analysis jaz proteins genome wide identification smjaz gene family eggplant smjaz high temperature
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Identification of Rice Leaf Width Gene FLW11 Through Genome-Wide Association Study and Functional Analysis 认领 引用
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作者 YANG Yulu ZHANG Yanfang +14 位作者 LIU Xiong ZHANG Lihua HUANG Jingfen SHEN Lixing ZHAO Huibo SHEN Lan ZHANG Qiang ZHU Li HU Jiang REN Deyong GAO Zhenyu DONG Guojun QIAO Weihua QIAN Qian ZHANG Guangheng 《Rice science》 SCIE CSCD 2025年第6期756-760,I0018-I0023,共5页
The width of rice leaves determines the size of the photosynthetic area.Optimizing rice leaf width can improve the photosynthetic rate,thereby increasing rice yield.In this study,a genome-wide association study(GWAS)w... The width of rice leaves determines the size of the photosynthetic area.Optimizing rice leaf width can improve the photosynthetic rate,thereby increasing rice yield.In this study,a genome-wide association study(GWAS)was conducted by 225 rice germplasm resources to explore the genetic basis of rice flag leaf width(FLW).We identified nine QTLs associated with FLW(qFLWs),with phenotypic contribution rates ranging from 3.17%to 14.37%.Near-isogenic lines(NILs)were developed for fine-mapping of qFLW11,and the function of FLW11 was further verified.We narrowed down q FLW11 to an 87-kb interval,which contains five genes. 展开更多
关键词 functional analysis genome wide association study rice leaf width flag leaf width rice germplasm resources qtl photosynthetic rate phenotypic contribution rates
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Genome-wide identification and expression profiling of the bZIP gene family in Chrysanthemum indicum and the functional analysis of CibZIP29 under cadmium stress 认领 引用
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作者 Shengyan Chen Yin Zhang +3 位作者 Kaiyuan Zhang Shuguang Liu Yujia Yang Xingnong Xu 《Ornamental Plant Research》 2025年第1期333-349,共17页
Recent industrial activities have increased cadmium(Cd)contamination in soils,negatively affecting crop growth and agricultural development.Chrysanthemum indicum represents a significant wild species within the Chrysa... Recent industrial activities have increased cadmium(Cd)contamination in soils,negatively affecting crop growth and agricultural development.Chrysanthemum indicum represents a significant wild species within the Chrysanthemum genus.Its distinctive resistance and adaptability serve as valuable references for the enhancement of ornamental chrysanthemum breeding programs.However,the role of bZIP family of Cd stress in C.indicum remains unclear.This study conducted a genome-wide identification and expression analysis of bZIP genes in C.indicum,identifying 65 CibZIP genes distributed across nine chromosomes.Phylogenetic analysis grouped them into 12 subgroups,supported by conserved motifs,gene,and protein structure analyses.Tandem and segmental duplications were found to be key drivers of CibZIP gene family expansion,with Ka/Ks analysis indicating purifying selection of duplicated genes.Promoter analysis revealed cis-acting elements involved in plant development and stress response.Transcriptomic data showed that CibZIP genes are highly expressed in different tissues and play significant roles in Cd stress tolerance.RT-qPCR analysis further confirmed that specific CibZIP genes,especially CibZIP29,are upregulated in response to Cd stress.Overexpression of CibZIP29 in transgenic Arabidopsis enhanced Cd tolerance,providing new insights into the molecular mechanisms underlying Cd stress response in C.indicum.These findings contribute to understanding the role of CibZIP genes in Cd tolerance and will support future research on Cd stress mechanisms in Chrysanthemum. 展开更多
关键词 enhancement ornamental chrysanthemum functional analysis bzip family Chrysanthemum indicum BZIP gene family cadmium stress promoter analysis genome wide identification
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Functional analysis of MeFER4 in regulating ROS homeostasis during abiotic stress responses in Arabidopsis 认领 引用 被引量：1
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作者 Mingjuan Zheng Jianjun Li +10 位作者 Chenyu Lin Bin Wang Pingjuan Zhao Feilong Yu Xianhai Xie Ming Peng Fei Chen Chengcheng Si Wenquan Wang Xiaohui Yu Xin Guo 《Tropical Plants》 CSCD 2025年第1期312-324,共13页
The MeFER4 gene in cassava(Manihot esculenta Crantz)encodes a ferritin-like protein involved in stress responses,particularly under drought and oxidative stress.This study explores the role of MeFER4 in regulating oxi... The MeFER4 gene in cassava(Manihot esculenta Crantz)encodes a ferritin-like protein involved in stress responses,particularly under drought and oxidative stress.This study explores the role of MeFER4 in regulating oxidative stress in transgenic Arabidopsis.Overexpression of MeFER4 enhanced sensitivity to various stresses,including drought,oxidative,salt,and osmotic stress,with increased levels of reactive oxygen species(ROS)and altered expression of ROSrelated genes in Arabidopsis.Furthermore,interactions between MeFER4 and antioxidant enzymes(APX1 and APX3),suggest its role in managing ROS homeostasis.These findings highlight MeFER4 as a key regulator of oxidative stress responses,offering the potential for improving stress tolerance in crops. 展开更多
关键词 Arabidopsis transgenic arabidopsisoverexpression regulating ROS homeostasis functional analysis oxidative stressthis reactive oxygen species ros abiotic stress responses oxidative stress
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Mechanisms driving surface wind speed increases in an ecologically fragile region of Northwest China:Insights from circulation anomalies and geographical detector analysis 认领 引用
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作者 WANG Yongliang ZHANG Weijiang 《Journal of Arid Land》 SCIE CAS CSCD 2026年第6期928-948,共21页
The Shaanxi-Gansu-Ningxia(SGN)border region,as a typical transitional zone between the East Asian monsoon and the westerlies,features a fragile ecological environment.In this study,the spatiotemporal evolution and mul... The Shaanxi-Gansu-Ningxia(SGN)border region,as a typical transitional zone between the East Asian monsoon and the westerlies,features a fragile ecological environment.In this study,the spatiotemporal evolution and multiscale driving mechanisms of surface winds in this ecologically fragile transition zone were investigated by integrating trend analysis,empirical orthogonal function(EOF)decomposition,and geographic detector method.The results indicated that from 1980 to 2022,the annual mean surface wind speeds in the SGN border region significantly increased,with a linear growth rate of 0.003 m/(s•a).However,the anomaly series revealed a clear interdecadal transition:surface wind speed anomalies were predominantly negative from 1980 to 1999 and shifted to persistently positive and increasing anomalies after 2000.Consistent strengthening was observed in summer,autumn,and winter,with the most pronounced increase occurring in autumn.The spatial distribution generally followed a pattern of higher values in the northwest and lower values in the southeast.Spring presented the strongest surface wind speeds and the most extensive areas with high values.EOF analysis revealed two dominant spatial modes:the first mode(variance contribution>73.40%)reflected regionally consistent changes,and its temporal coefficients increased continuously,corresponding to the overall strengthening trend of surface wind speed;and the second mode exhibited an east‑west dipole oscillation pattern,dominated by interannual fluctuations.The geographic detector results revealed that fractional vegetation cover(FVC),temperature,and topographic elevation were key factors influencing the spatial differentiation of surface wind speeds,with all the factors exhibiting enhanced interactive effects—especially the synergistic effect between vegetation cover and temperature.Background circulation analysis indicated that enhanced westerlies and decreased geopotential height in the mid‑to upper‑troposphere provided favourable dynamic conditions for increased surface wind speeds.This study advances the understanding of surface wind speed changes in climate transition zones,providing a scientific basis for regional wind energy planning,ecological protection,and wind erosion control. 展开更多
关键词 surface wind speed Shaanxi-Gansu-Ningxia(SGN)region empirical orthogonal function(EOF)analysis geographical detector atmospheric circulation
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Analysis of the regional spectral properties in northwestern South China Sea based on an empirical orthogonal function 认领 引用 被引量：4
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作者 Dongyang Fu Yuye Huang +3 位作者 Dazhao Liu Shan Liao Guo Yu Xiaolong Zhang 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2020年第7期107-114,共8页
This study presents an analysis of the spectral characteristics of remote sensing reflectance(Rrs)in northwestern South China Sea based on the in situ optical and water quality data for August 2018.Rrswas initially di... This study presents an analysis of the spectral characteristics of remote sensing reflectance(Rrs)in northwestern South China Sea based on the in situ optical and water quality data for August 2018.Rrswas initially divided into four classes,classes A to D,using the max-classification algorithm,and the spectral properties of whole Rrs were characterized using the empirical orthogonal function(EOF)analysis.Subsequently,the dominant factors in each EOF mode were determined.The results indicated that more than 95%of the variances of Rrs are partly driven by the back-scattering characteristics of the suspended matter.The initial two EOF modes were well correlated with the total suspended matter and back-scattering coefficient.Furthermore,the first EOF modes of the four classes of Rrs(A-D Rrs-EOF1)significantly contributed to the total variances of each Rrs class.In addition,the correlation coefficients between the amplitude factors of class A-D Rrs-EOF1 and the variances of the relevant water quality and optical parameters were better than those of the unclassified ones.The spectral shape of class ARrs-EOF1 was governed by the absorption characteristic of chlorophyll a and colored dissolved organic matter(CDOM).The spectral shape of class B Rrs-EOF1 was governed by the absorption characteristic of CDOM since it exhibited a high correlation with the absorption coefficient of CDOM(ag(λ)),whereas the spectral shape of class C Rrs-EOF1 was governed by the back-scattering characteristics but not affected by the suspended matter.The spectral shape of class D Rrs-EOF1 exhibited a relatively good correlation with all the water quality parameters,which played a significant role in deciding its spectral shape. 展开更多
关键词 northwestern South China Sea remote sensing reflectance empirical orthogonal function analysis ocean color control factors
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A simple and efficient gene functional analysis method for studying the growth and development of peach seedlings 认领 引用 被引量：2
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作者 Jun Cheng Yun Shao +11 位作者 Xinyue Hu Liying Gao Xianbo Zheng Bin Tan Xia Ye Wei Wang Haipeng Zhang Xiaobei Wang Xiaodong Lian Zhiqian Li Jiancan Feng Langlang Zhang 《Horticulture Research》 SCIE CSCD 2024年第7期379-387,共9页
Stable genetic transformation of peach[Prunus persica(L.)Batsch]still faces many technical challenges,and existing transient expression methods are limited by tissue type or developmental stage,making it difficult to ... Stable genetic transformation of peach[Prunus persica(L.)Batsch]still faces many technical challenges,and existing transient expression methods are limited by tissue type or developmental stage,making it difficult to conduct functional analysis of genes regulating shoot growth.To overcome this dilemma,we developed a three-step method for efficient analysis of gene functions during peach seedling growth and development.This method resulted in transformation frequencies ranging from 48 to 87%,depending on the gene.From transformation of germinating seeds to phenotyping of young saplings took just 1.5 months and can be carried out any time of year.To test the applicability of this method,the function of three tree architecture-related genes,namely PpPDS,PpMAX4,and PpWEEP,and two lateral root-related genes,PpIAA14–1 and−2,were confirmed.Since functional redundancy can challenge gene functional analyses,tests were undertaken with the growth-repressor DELLA,which has three homologous genes,PpDGYLA(DG),PpDELLA1(D1),and−2(D2),in peach that are functionally redundant.Silencing using a triple-target vector(TRV2-DG-D1-D2)resulted in transgenic plants taller than those carrying just TRV2-DG or TRV2.Simultaneously silencing the three DELLA genes also attenuated the stature of two dwarf genotypes,‘FHSXT’and‘HSX’,which normally accumulate DELLA proteins.Our study provides a method for the functional analysis of genes in peach and can be used for the study of root,stem,and leaf development.We believe this method can be replicated in other woody plants. 展开更多
关键词 transformation frequencies stable genetic transformation gene functional analysis functional analysis peach seedlings analysis gene functions transient expression methods
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Inflorescence characteristics as function-valued traits:Analysis of heritability and selection on architectural effects 认领 引用 被引量：2
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作者 Mason W.Kulbaba Ilona C.Clocher Lawrence D.Harder 《Journal of Systematics and Evolution》 SCIE CAS CSCD 2017年第6期559-565,共7页
Production of multiple flowers in inflorescences allows the reproductive phenotypes of individual plants to include systematic among-flower variation,which could be adaptive.Systematic trait variation within infloresc... Production of multiple flowers in inflorescences allows the reproductive phenotypes of individual plants to include systematic among-flower variation,which could be adaptive.Systematic trait variation within inflorescences could arise from resource competition among flowers,or be a developmentally determined feature of flower position,regardless of resource dynamics.The latter,architectural effect typically manifests as continuous floral variation within inflorescences.For architectural effects to be adaptive,floral trait variation among individuals must covary with reproductive performance and be heritable.However,heritability and phenotypic selection on gradients of variation cannot be estimated readily with traditional statistical approaches,instead,we advocate and illustrate the application of two functional data analysis techniques with observations of Delphinium g/aucum(Ranunculaceae).To demonstrate the parameters-as-data approach we quantify heritability of variation in anthesis rate,as represented by the regression coefficient relating daily anthesis rate to inflorescence age.SNP-based estimates detected significant heritability(h2=0.245)for declining anthesis rate within inflorescences.Functional regression was used to assess phenotypic selection on anthesis rate and a floral trait(lower sepal length).The approach used spline curves that characterize within-inflorescence variation as functional predictors of a plant's fruit set.Selection on anthesis rate varied with inflorescence age and the duration of an individual's anthesis period.Lower sepal length experienced positive selection for basa]and distal flowers,but negative selection for central flowers.These results illustrate the utility and power of functional-data analyses for studying architectural effects and specifically demonstrate that these effects are subject to natural selection and hence adaptive. 展开更多
关键词 architectural effects functional data analysis heritability inflorescence display selection.
Genome-wide identification of bHLH transcription factors and functional analysis in salt gland development of the recretohalophyte sea lavender(Limonium bicolor) 认领 引用 被引量：1
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作者 Xi Wang Baoshan Wang Fang Yuan 《Horticulture Research》 SCIE CSCD 2024年第4期29-39,共11页
Transcription factors with basic helix–loop–helix(bHLH)structures regulate plant growth,epidermal structure development,metabolic processes,and responses to stress extensively.Sea lavender(Limonium bicolor)is a recr... Transcription factors with basic helix–loop–helix(bHLH)structures regulate plant growth,epidermal structure development,metabolic processes,and responses to stress extensively.Sea lavender(Limonium bicolor)is a recretohalophyte with unique salt glands in the epidermis that make it highly resistant to salt stress,contributing to the improvement of saline lands.However,the features of the bHLH transcription factor family in L.bicolor are largely unknown.Here,we systematically analyzed the characteristics,localization,and phylogenetic relationships of 187 identified bHLH family genes throughout the L.bicolor genome,as well as their cis-regulatory promoter elements,expression patterns,and key roles in salt gland development or salt tolerance by genetic analysis.Nine verified L.bicolor bHLH genes are expressed and the encoded proteins function in the nucleus,among which the proteins encoded by Lb2G14060 and Lb1G07934 also localize to salt glands.Analysis of CRISPR-Cas9-generated knockout mutants and overexpression lines indicated that the protein encoded by Lb1G07934 is involved in the formation of salt glands,salt secretion,and salt resistance,indicating that bHLH genes strongly influence epidermal structure development and stress responses.The current study lays the foundation for further investigation of the effects and functional mechanisms of bHLH genes in L.bicolor and paves the way for selecting salt-tolerance genes that will enhance salt resistance in crops and for the improvement of saline soils. 展开更多
关键词 bhlh transcription factor cis regulatory elements salt gland development functional analysis transcription factors genome wide identification Limonium bicolor salt glands
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Functional data analysis-based yield modeling in year-round crop cultivation 认领 引用 被引量：2
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作者 Hidetoshi Matsui Keiichi Mochida 《Horticulture Research》 SCIE CSCD 2024年第7期200-206,共7页
Crop yield prediction is essential for effective agricultural management.We introduce a methodology for modeling the relationship between environmental parameters and crop yield in longitudinal crop cultivation,exempl... Crop yield prediction is essential for effective agricultural management.We introduce a methodology for modeling the relationship between environmental parameters and crop yield in longitudinal crop cultivation,exemplified by strawberry and tomato production based on year-round cultivation.Employing functional data analysis(FDA),we developed a model to assess the impact of these factors on crop yield,particularly in the face of environmental fluctuation.Specifically,we demonstrated that a varying-coefficient functional regression model(VCFRM)is utilized to analyze time-series data,enabling to visualize seasonal shifts and the dynamic interplay between environmental conditions such as solar radiation and temperature and crop yield.The interpretability of our FDA-based model yields insights for optimizing growth parameters,thereby augmenting resource efficiency and sustainability.Our results demonstrate the feasibility of VCFRM-based yield modeling,offering strategies for stable,efficient crop production,pivotal in addressing the challenges of climate adaptability in plant factory-based horticulture. 展开更多
关键词 assess impact factors modeling relationship environmental parameters effective agricultural managementwe varying coefficient functional regression model environmental parameters functional data analysis crop yield prediction functional data analysis fda we
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Protein profile of human hepatocarcinoma cell line SMMC-7721:Identification and functional analysis 认领 引用 被引量：9
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作者 Yi Feng Zhong-Min Tian Ming-Xi Wan Zhao-Bin Zheng 《World Journal of Gastroenterology》 SCIE CAS 2007年第18期2608-2614,共7页
AIM： TO investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasi... AIM： TO investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to explore the new drug targets for liver cancer therapy. METHODS： Total proteins from human hepatocarcinoma cell line SMMC-7721 were separated by two-dimensional electrophoresis （2DE）. The silver-stained gel was analyzed by 2DE software Image Master 2D Elite. Interesting protein spots were identified by peptide mass fingerprinting based on matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and database searching. RESULTS： We obtained protein profile of human hepatocarcinoma cell line SMMC-7721. Among the twenty-one successfully identified proteins, mitofilin, endoplasmic reticulum protein ERp29, ubiquinol-cytochrome C reductase complex core protein I, peroxisomal enoyl CoA hydratase, peroxiredoxin-4 and probable 3-oxoacid CoA transferase 1 precursor were the six novel proteins identified in human hepatocarcinoma cells or tissues. Specific functions of the identified heat-shock proteins were analyzed in detail, and the results suggested that these proteins might promote tumorigenesis via inhibiting cell death induced by several cancer-related stresses or via inhibiting apoptosis at multiple points in the apoptotic signal pathway. Other identified chaperones and cancer-related proteins were also analyzed.CONCLUSION： Based on the protein profile of SMMC-7721 cells, functional analysis suggests that the identified chaperones and cancer-related proteins have their own pathways to contribute to the tumorigenesis, tumor growth and metastasis of liver cancer. Furthermore, proteomic analysis is indicated to be feasible in the cancer study. 展开更多
关键词 Human hepatocarcinoma cell line SMMC-7721 Protein identification Functional analysis Heat-shockprotein Tumorigenesis
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Semen analysis and sperm function testing 认领 引用 被引量：11
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作者 Daniel R Franken Sergio Oehninger 《Asian Journal of Andrology》 SCIE CAS CSCD 2012年第1期6-13,174,共8页
Despite controversy regarding the clinical value of semen analysis, male fertility investigation still relies on a standardized analysis of the semen parameters. This is especially true for infertility clinics in both... Despite controversy regarding the clinical value of semen analysis, male fertility investigation still relies on a standardized analysis of the semen parameters. This is especially true for infertility clinics in both developing and developed countries. Other optional tests or sophisticated technologies have not been widely applied. The current review addresses important changes in the analysis of semen as described in the new World Health Organization （WHO） manual for semen analysis. The most important change in the manual is the use of evidence-based publications as references to determine cutoff values for normality. Apart from the above mentioned changes, the initial evaluation and handling methods remain, in most instances, the same as in previous editions. Furthermore, the review evaluates the importance of quality control in andrology with emphasis on the evaluation of sperm morphology. WHO sperm morphology training programmes for Sub-Saharan countries were initiated at Tygerberg Hospital in 1995. The external qualitY control programme has ensured that the majority of participants have maintained their morphological reading skills acquired during initial training. This review reports on current sperm functional tests, such as the induced acrosome reaction, and sperm-zona pellucida binding assays, as well as the impact of sperm quality in terms of DNA integrity, and the relationship of sperm function tests to sperm morphology. 展开更多
关键词 male infertility quality assurance semen analysis spermatozoa sperm concentration sperm function sperm morphology sperm motility
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Isolation,expression and functional analysis of the GbGGPPS1 gene in Ginkgo biloba 认领 引用 被引量：1
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作者 Qingjie Wang Xiya Zhao +1 位作者 Yang Jiang Li Wang 《Fruit Research》 2023年第1期1-9,共9页
Ginkgo biloba is known for its abundance of secondary metabolites,such as terpenoids.However,the identification and functional analysis of key genes in the terpenoid biosynthetic pathway are limited.Geranylgeranyl pyr... Ginkgo biloba is known for its abundance of secondary metabolites,such as terpenoids.However,the identification and functional analysis of key genes in the terpenoid biosynthetic pathway are limited.Geranylgeranyl pyrophosphate synthase(GGPPS)is a crucial enzyme involved in the production of terpenoids.In this study,we successfully cloned the GbGGPPS1 gene from G.biloba.The length of GbGGPPS1 was 1,176 bp,encoding 391 amino acids.The GbGGPPS1 protein contains the GGPPS protein domain,including the FARM and SAR motifs.Phylogenetic analysis revealed that GbGGPPS1 is closely related to gymnosperms.Furthermore,the expression of GbGGPPS1 was higher in leaves,stems,and ovules compared to the roots.Additionally,overexpression of GbGGPPS1 in Arabidopsis increased chlorophyll content and accelerated flowering process,indicating its role as a terpenoid synthase gene and plant development.Notably,the expression of GbGGPPS1 can be induced by salt stress,and overexpression of GbGGPPS1 in Arabidopsis exhibited enhanced seed germination under salt treatment.These results indicate that GbGGPPS1 plays an important role in plant growth,development,and salt stress resistance. 展开更多
关键词 pyrophosphate synthase ggpps ginkgo biloba expression amino acidsthe functional analysis gbggpps gene farm sa secondary metabolitessuch
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Comprehensive Reliability Allocation Method for CNC Lathes Based on Cubic Transformed Functions of Failure Mode and Effects Analysis 认领 引用 被引量：9
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作者 YANG Zhou ZHU Yunpeng +1 位作者 REN Hongrui ZHANG Yimin 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2015年第2期315-324,共10页
Reliability allocation of computerized numerical controlled(CNC)lathes is very important in industry.Traditional allocation methods only focus on high-failure rate components rather than moderate failure rate componen... Reliability allocation of computerized numerical controlled(CNC)lathes is very important in industry.Traditional allocation methods only focus on high-failure rate components rather than moderate failure rate components,which is not applicable in some conditions.Aiming at solving the problem of CNC lathes reliability allocating,a comprehensive reliability allocation method based on cubic transformed functions of failure modes and effects analysis(FMEA)is presented.Firstly,conventional reliability allocation methods are introduced.Then the limitations of direct combination of comprehensive allocation method with the exponential transformed FMEA method are investigated.Subsequently,a cubic transformed function is established in order to overcome these limitations.Properties of the new transformed functions are discussed by considering the failure severity and the failure occurrence.Designers can choose appropriate transform amplitudes according to their requirements.Finally,a CNC lathe and a spindle system are used as an example to verify the new allocation method.Seven criteria are considered to compare the results of the new method with traditional methods.The allocation results indicate that the new method is more flexible than traditional methods.By employing the new cubic transformed function,the method covers a wider range of problems in CNC reliability allocation without losing the advantages of traditional methods. 展开更多
关键词 CNC lathes reliability failure modes and effects analysis(FMEA)cubic transformed function comprehensive allocation
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Genome-wide analysis of the S-phase kinase-association protein1(ClSKP1) family and the role of S-RNase targeting by an SCF(Cullin1-SKP1-F-box) complex in the self-incompatibility of‘Xiangshui' lemon 认领 引用 被引量：1
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作者 Yuze Li Wei Lin +9 位作者 Jiawei Zhu Moying Lan Cong Luo Yili Zhang Rongzhen Liang Liming Xia Wangli Hu Xiao Mo Guixiang Huang Xinhua He 《Horticultural Plant Journal》 SCIE CAS CSCD 2025年第2期593-607,共15页
The SKP1 gene is an important component of the SCF(SKP1-Cullin1-F-box)complex and serves as a bridge connecting the F-box and Cullin1genes(F-box-SKP1-Cullin1).The pattern of S-RNase being ubiquitously labelled by the ... The SKP1 gene is an important component of the SCF(SKP1-Cullin1-F-box)complex and serves as a bridge connecting the F-box and Cullin1genes(F-box-SKP1-Cullin1).The pattern of S-RNase being ubiquitously labelled by the SCF complex and degraded by the 26S protease accounts for the bulk of the available self-incompatibility studies.In this study,15 ClSKP1s from the‘Xiangshui'lemon genome and ubiquitome exist in the same SKP1 conserved domain(CD)as SKP1s in other species.The q PCR results showed that SKP1-6 and SKP1-14 have tissue expression patterns specific for expression in pollen.In addition,SKP1-6 and SKP1-14 in the stigma,style and ovary were significantly upregulated after self-pollination compared to those after cross-pollination.A subcellular location showed that SKP1-6 and SKP1-14 were located in the nucleus.In addition,yeast two-hybrid(Y2H)assays,bimolecular fluorescence complementation(BiFC)and luciferase complementation imaging(LCI)assays showed that SKP1-6 interacted with F-box1,F-box33,F-box34,F-box17,F-box19,Cullin1-2 and 26S proteasome subunit 4 homolog A(26S PS4HA).SKP1-14 interacted with F-box17,F-box19,F-box35,Cullin1-2 and 26S PS4HA.The interaction of Cullin1-2 and the F-box with SKP1 as a bridge was verified by a yeast three-hybrid experiment.The ability of S3-RNase to inhibit pollen and pollen tube growth and development was assessed using in vitro pollen co-culture experiments with recombinant S3-RNase proteins.Overall,this study provides important experimental evidence and theoretical basis for understanding the mechanism of self-incompatibility in plants by revealing the key role of the SCF complex in‘Xiangshui'lemon,which is bridged by ClSKP1-6,in self-incompatibility.The results of this study are of great significance for the future indepth exploration of the molecular mechanism of the SCF complex and its wide application in the self-incompatibility of plants. 展开更多
关键词 Lemon SKP1 SCF Self-incompatibility Expression analysis Functional analysis
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