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High genetic load and recent anthropogenic bottleneck in the endangered Cupressus austrotibetica(Cupressaceae),Asia's tallest tree 认领 引用 被引量：1
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作者 Heng Yang Jialiang Li +10 位作者 Mi Yoon Chung Myong Gi Chung Zhitong Han Dayu Wu Jingge Kuang Xinran Zhang Xi Zhou Linning Bai Jianquan Liu Jian Luo Kangshan Mao 《Journal of Systematics and Evolution》 SCIE CSCD 2026年第1期125-137,共13页
Understanding the genetic diversity and genetic load of endangered species is essential for developing effective conservation strategies,particularly in ecologically sensitive regions such as the Himalayas.Cupressus a... Understanding the genetic diversity and genetic load of endangered species is essential for developing effective conservation strategies,particularly in ecologically sensitive regions such as the Himalayas.Cupressus austrotibetica,a rare conifer and the tallest recorded tree in Asia,reaching up to 102.3 m,faces substantial anthropogenic and environmental threats.To evaluate its genetic status,we sequenced transcriptomes of 54 individuals sampled across its restricted range and compared them with 96 individuals of C.gigantea,a closely related endangered species with broader distribution at higher elevations.Our analysis reveals that C.austrotibetica exhibits higher genetic diversity(π=0.0091)compared to C.gigantea(π=0.0042).Demographic analyses identified three historical bottleneck events in C.austrotibetica and two in C.gigantea,with two of these events coinciding with Quaternary climatic oscillations.Despite its relatively high genetic diversity,C.austrotibetica has a smaller effective population size based on Stairway Plot 2(Ne≈7200)than C.gigantea(Ne≈17600).Furthermore,C.austrotibetica harbors a higher proportion of severe deleterious mutations,while C.gigantea retains more moderate deleterious variants.These findings indicate that a recent anthropogenic bottleneck event has likely driven the reduced population size and increased genetic load in C.austrotibetica,emphasizing the urgent need for conservation priorities for this imperiled species. 展开更多
关键词 conservation endangered species genetic diversity genetic load small population
ddRAD sequencing of 1076 Camellia accessions reveals the genetic diversity and population introgression of the tea plant in China 认领 引用 被引量：3
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作者 Xiao-Yan Tong Jian-Bing Hu +14 位作者 Hui-Lin Wen Chun-Lin Chen Ling-Ling Tao Jun Wu Yi-Ping Tian Hui-Ping Jiang Lin-Bo Chen Da-He Qiao Ming-Tao Shu En-Hua Xia Kun Dong Yue Fei Sheng-Rui Liu Chao-Ling Wei Jun-Yan Zhu 《Plant Diversity》 SCIE CAS CSCD 2026年第3期451-459,共9页
The tea plant(Camellia sinensis)is a widely cultivated and economically significant crop that originated from southwestern China.Owing to its high heterozygosity and complex genetic diversity,the evolutionary history ... The tea plant(Camellia sinensis)is a widely cultivated and economically significant crop that originated from southwestern China.Owing to its high heterozygosity and complex genetic diversity,the evolutionary history of the tea plant and its domestication remain unclear.In this study,we used 1076 Camellia accessions to elucidate the phylogenetic relationships and population structure of Camellia accessions across 16 major tea-producing provinces in China.Six species-specific populations were classified based on these SNPs through phylogenetic trees and population structure.We found that C.taliensis populations have undergone divergent evolution compared to other wild relatives,such as C.kwangsiensis and C.crassicoluma.Population structure and introgression analyses further revealed frequent interspecific genetic exchanges between C.sinensis and its wild relatives.Thus,a transitional C.sinensis population that shared a genetic background with C.taliensis was characterized.Distinct genetic divergence was observed within the two main varieties of C.sinensis.Contrary to the relatively exclusive genetic background within the C.sinensis var.assamica population in southwestern China,extensive regional introgression and gene flow were observed in the C.sinensis var.sinensis population between southwestern and eastern China.Finally,we developed a core collection comprising 160 accessions based on our genetic diversity analyses.Our findings not only provide insights into the evolutionary history and genetic diversity of the tea plant but may facilitate the development of the conservation and utilization of tea plant resources. 展开更多
关键词 Tea plant Population structure ddRAD-seq Genetic diversity Core collection Introgression
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Spatially biased collections and the failure to cover all wild genetic clusters in plant populations under ex situ conservation 认领 引用 被引量：1
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作者 Zhiqiang Xiao Hui Liu +5 位作者 Guiyun Huang Di Wu Liwen Qiu Jinhua Wu Xinzeng Wei Mingxi Jiang 《Plant Diversity》 SCIE CAS CSCD 2026年第1期75-83,共9页
Successful ex situ conservation of plant populations requires a high degree of genetic representativeness.However,spatially biased sampling in ex situ conservation efforts may fail to capture all wild genetic clusters... Successful ex situ conservation of plant populations requires a high degree of genetic representativeness.However,spatially biased sampling in ex situ conservation efforts may fail to capture all wild genetic clusters for species with range-wide genetic structure.To investigate the extent of spatially biased sampling in living collections and the coverage of wild genetic clusters in plant populations under ex situ conservation worldwide,we combined a global synthesis of ex situ conservation efforts with a case study of an endangered riparian plant species,Myricaria laxiflora.Our analysis of ex situ conservation worldwide revealed that the majority(82.6%)of ex situ populations fail to cover all wild genetic clusters,largely due to spatially biased sampling with low geographic coverage.Our case study of M.laxiflora showed that genetic diversity differed between the ex situ and upstream populations,while it was comparable between ex situ populations and other wild populations.However,current ex situ populations did not cover all wild genetic clusters,as the upstream genetic cluster was previously uncollected.Our study suggests that the failure to cover all wild genetic clusters in ex situ populations is a widespread issue,and ex situ populations with high genetic diversity can also fail to cover all wild genetic clusters.In future ex situ conservation programs,both the importance of high genetic diversity and the high coverage of wild genetic clusters should be prioritized. 展开更多
关键词 Conservation genomics Genetic representativeness Ex situ conservation Genetic composition Geographic coverage Spatially biased sampling
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Recent advances in genomic studies for domestication and genetic improvement of traits in goats 认领 引用 被引量：1
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作者 Zhanerke Akhatayeva Hongying Dan +5 位作者 Hosein Salehian-Dehkordi Talgat Seiteuov Abdugani Abdurasulov Rustembay Aitjanov Kejian Lin Songsong Xu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第6期2268-2287,共20页
Goats(Capra hircus)provide a rich source of products,such as meat,milk,and wool,and are important domestic animals in many parts of the world.Goats were one of the first domesticated livestock species during the late ... Goats(Capra hircus)provide a rich source of products,such as meat,milk,and wool,and are important domestic animals in many parts of the world.Goats were one of the first domesticated livestock species during the late Neolithic period,approximately 11,000 years ago,in the Fertile Crescent.In the past decades,genomic studies of goats have provided insight into their domestication and genetic basis of economically important traits.This review outlines the latest advancements that have been made in reference to domestication and genetic improvement of production traits such as meat and carcass quality,reproduction,milk,cashmere,and functional traits such as environmental adaptation and diseaseresistance.Genomic research is entering a new era with the availability of graphical pan-genomics and telomere-totelomere(T2T)gap-free genome assembly,which will extend our understanding of domestication and molecular mechanistic dissection of economic traits in goats.We provide new perspectives and future directions for genomics and suggest how the ever-increasing multi-omics dataset will facilitate future studies and molecular breeding in goat. 展开更多
关键词 goat genomics genes domestication genetic improvement
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Harness the wild:progress and perspectives in wheat genetic improvement 认领 引用 被引量：1
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作者 Xiubin Tian Ziyu Wang +1 位作者 Wenxuan Liu Yusheng Zhao 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2026年第1期1-15,共15页
Bread wheat(Triticum aestivum L.)is a staple hexaploid crop with numerous wild relatives.However,domestication and modern breeding have significantly narrowed its genetic diversity,diminishing its capacity to adapt to... Bread wheat(Triticum aestivum L.)is a staple hexaploid crop with numerous wild relatives.However,domestication and modern breeding have significantly narrowed its genetic diversity,diminishing its capacity to adapt to climate change.Wild relatives of wheat serve as a vital reservoir of genetic diversity,offering traits thatenhance its resistance to various biotic and abiotic stresses.Over recent decades,remarkable progress has been made in utilizing superior genes from wild relatives to bolster wheat's defenses against diseases and pests,though the exploration of genes conferring abiotic stress tolerance has lagged behind.In this review,we summarize key advancements in the utilization of wild relatives for wheat enhancement over the past century,emphasizing both theoretical and technological innovations.Furthermore,we evaluate the potential contributions of wild relatives to address production challenges posed by climate change.We also explore strategies for isolating superior genes and developing prebreeding germplasm to support the future development of climate-resilient wheat varieties. 展开更多
关键词 Bread wheat Wild relatives Biotic stress Abiotic stress Genetic improvement Climate change
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Genetic landscape of hereditary cardiomyopathies and arrhythmias in China 认领 引用
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作者 Yang Lu Zeyuan Wang +4 位作者 Shuyuan Zhang Yaping Liu Ye Jin Zhuang Tian Shuyang Zhang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2026年第2期246-255,共10页
Hereditary cardiomyopathies and arrhythmias are major contributors to cardiovascular morbidity and mortality.The advent of next-generation sequencing(NGS)has made genetic testing more accessible,which is crucial for p... Hereditary cardiomyopathies and arrhythmias are major contributors to cardiovascular morbidity and mortality.The advent of next-generation sequencing(NGS)has made genetic testing more accessible,which is crucial for precise diagnosis and targeted therapeutic strategies.The aim of this study is to explore the landscape of genetic variants,the relationship between specific variants and clinical phenotypes,and the impact on clinical decision-making in China.A total of 1536 probands(median age,37 years;1025 males[66.7%])with suspected hereditary cardiomyopathy or arrhythmia(covering 15 clinical phenotypes)are recruited from 146 hospitals across 30 provinces and cities in China.Positive results are confirmed in 390 of 1536 probands,leading to a diagnostic yield of 25.4%.Forty-two and three-tenths percent(n=169)of family members carry the same variants as positive probands.Hypertrophic cardiomyopathy(HCM)and dilated cardiomyopathy(DCM)are the predominant phenotypes,with MYBPC3 variants having the highest frequency in HCM and TTN variants in DCM.In 76.9%of the positive probands,the identified variants are helpful in clinical management,family screening,and fertility.This large-scale study provides significant insights into the genetic landscape of hereditary cardiomyopathies and arrhythmias in China. 展开更多
关键词 Genetic testing Cardiomyopathy Arrhythmia Diagnostic yield Chinese population Cardiovascular genetics
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Shared genetic link and causal inference between blood lipids,lipid-lowering drugs and amyotrophic lateral sclerosis 认领 引用
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作者 Kailin Xia Ninghao Huang +7 位作者 Yajun Wang Gan Zhang Lu Tang Linjing Zhang Minhao Yao Zhonghua Liu Tao Huang Dongsheng Fan 《Neural Regeneration Research》 SCIE CAS CSCD 2026年第8期3824-3830,共7页
Growing evidence suggests that abnormal lipid metabolism occurs in amyotrophic lateral sclerosis,even in the presymptomatic stage,implying an etiologic link.However,the genetic mechanism underlying altered lipid level... Growing evidence suggests that abnormal lipid metabolism occurs in amyotrophic lateral sclerosis,even in the presymptomatic stage,implying an etiologic link.However,the genetic mechanism underlying altered lipid levels in amyotrophic lateral sclerosis remains elusive.Therefore,in this study,we performed genetic correlation analysis,a cross-trait meta-analysis,tissue-specific enrichment analysis,and bidirectional two-sample Mendelian randomization analysis of European population to explore whether there is a genetic and causal relationship between lipids and amyotrophic lateral sclerosis.The effect of lipid-lowering drugs on amyotrophic lateral sclerosis was also evaluated using a drug target Mendelian randomization approach.The results showed a positive genetic correlation between amyotrophic lateral sclerosis and both high-density lipoprotein cholesterol and apolipoprotein A1 and identified 71 independent shared loci between amyotrophic lateral sclerosis and high-density lipoprotein cholesterol,as well as 55 independent shared loci between amyotrophic lateral sclerosis and apolipoprotein A1.These shared loci were enriched in the lipid metabolic pathway and the alcohol metabolic pathway.Further Mendelian randomization analysis targeting lipid-lowering drugs showed that single nucleotide polymorphisms within the ACLY and PCSK9 genes had a protective effect against amyotrophic lateral sclerosis risk by decreasing low-density lipoprotein cholesterol.The combination of ACLY and PCSK9 inhibitors has a greater protective effect on amyotrophic lateral sclerosis risk than that of PCSK9 inhibitors alone.In summary,there is a common genetic structure between lipids and amyotrophic lateral sclerosis.Mendelian randomization analysis supports an association between elevated blood lipids and the risk of developing amyotrophic lateral sclerosis,and the use of ACLY or PCSK9 inhibitors may improve disease prognosis. 展开更多
关键词 amyotrophic lateral sclerosis genetic correlation genetics instrumental variables lipid-lowering drug lipids Mendelian randomization metabolism nerve regeneration neurodegenerative disease risk factor
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The occurrence and genetic diversity of vegetable root-knot nematodes in Xinjiang Uygur Autonomous Region 认领 引用
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作者 Junhui Zhou Yuxuan Zhao +9 位作者 Wenfang Luo Hudie Shao Wei He Deliang Peng Wenkun Huang Huiqin Wang Honghai Zhao Jianjun Xu Huan Peng 无 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第6期2475-2484,共10页
Root-knot nematodes(RKNs)are the most economically damaging plant-parasitic nematodes globally.Xinjiang,encompassing one-sixth of China's landmass,currently lacks comprehensive data regarding the occurrence,distri... Root-knot nematodes(RKNs)are the most economically damaging plant-parasitic nematodes globally.Xinjiang,encompassing one-sixth of China's landmass,currently lacks comprehensive data regarding the occurrence,distribution,and genetic variation of RKNs infecting vegetables within its borders.Hence,identifying RKNs species and genetic diversity is crucial for devising comprehensive management strategies.Between 2021 and 2023,we present a survey of 130 samples,collected from 86 counties across 14 cities in Xinjiang,China,aiming to comprehensively understand the occurrence,distribution,damage,and species of vegetable RKNs.The results indicated that 57 out of 130 samples collected from the regions of Hami,Tulufan,Ili,Bayingol,Hotan,Aksu,Kashgar,and Kizilsu in Xinjiang were infected by RKNs,suggesting an expansion of RKN disease in the vegetable-producing regions of Xinjiang.The infected vegetable roots were found to harbor Meloidogyne incognita and M.hapla,with M.incognita being the most prevalent species.A phylogenetic analysis of the COI region revealed significant evolutionary divergence between M.incognita populations from Xinjiang and those from southeastern provinces.Haplotype analysis of the COI gene revealed that M.incognita populations are categorized into three major lineages:Asia,Europe,and a combined lineage encompassing both America and Africa.Notable gene fiow patterns were observed among M.incognita populations,with significant migrations from Europe and America to Asia,specifically from the southeastern China towards Xinjiang.This study's findings indicate a consistent increase in the detrimental effects of vegetables production caused by RKNs in Xinjiang.Implementing effective prevention and control measures is crucial to mitigate the spread of RKNs. 展开更多
关键词 root-knot nematode vegetables occurrence genetic diversity Xinjiang
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Global DNA methylation shifts in a somatic hybrid introgression line underscore the critical role of epigenetic regulation in cauliflower curd development 认领 引用
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作者 Liming Miao Mengmeng Duan +5 位作者 Ning Guo Xin Liu Mei Zong Guixiang Wang Shuo Han Fan Liu 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第5期1250-1254,共5页
Somatic hybridization offers a promising approach to enhance genetic diversity in cauliflower,a crop characterized by a narrow genetic base.In this study,we investigated epigenetic remodeling and associated phenotypic... Somatic hybridization offers a promising approach to enhance genetic diversity in cauliflower,a crop characterized by a narrow genetic base.In this study,we investigated epigenetic remodeling and associated phenotypic changes in the Brassica nigra-derived somatic hybrid introgression line‘Y15-15-116'and its recipient parent‘Korso'.‘Y15-15-116'exhibits early maturity,pale-green curd,and loose texture,whereas‘Korso'displays late maturity with white,compact curd.Combined analysis of wholegenome bisulfite sequencing and transcriptome revealed that somatic hybridization induced significant alterations in CHG and CHH methylation levels,particularly in transposable elements(TEs). 展开更多
关键词 epigenetic remodeling enhance genetic diversity genetic basein epigenetic regulation associated phenotypic changes wholegenome bisulfite sequencing DNA methylation somatic hybridization
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Unraveling genetic underpinnings of purine content in pork 认领 引用
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作者 Cong Huang Min Zheng +8 位作者 Yizhong Huang Liping Cai Xiaoxiao Zou Tianxiong Yao Xinke Xie Bin Yang Shijun Xiao Junwu Ma Lusheng Huang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第3期1099-1113,共15页
The significance of purine base content as an important nutrition indicator in foods arises from its potential to trigger hyperuricemia or gout via high-purine diet.Livestock meats,including pork,generally contain mod... The significance of purine base content as an important nutrition indicator in foods arises from its potential to trigger hyperuricemia or gout via high-purine diet.Livestock meats,including pork,generally contain moderate to high total purine content(TP).Recent research revealed substantial variations within and across pig breeds,implying genetic factors influencing this trait.Thus,this study aimed to unravel the genetic underpinnings governing purine base content in pork.The heritability estimates(h~2)for the four purine traits ranged from 0.14 to 0.35.A total of 14,36,19 and 25 quantitative trait loci(QTLs)were identified for guanine,adenine,hypoxanthine,and TP,respectively.Our comprehensive gene set enrichment analysis and gene network analysis revealed 15 promising candidate genes intricately interwoven within diverse purine metabolism pathways,such as purine ribonucleotide metabolic process,purine nucleotide metabolism and transport,and purine salvage pathways,all contributing to TP.Strikingly,most genetic variants significantly associated with TP displayed analogous effects on multiple purine bases.Two distinct and highly significant QTLs(P<10–12)emerged on Sus scrofa chromosome(SSC)12:one impacting guanine content and the other concurrently influencing adenine and hypoxanthine levels.The peak of the guanine QTL on SSC12 resided 1.1 kb downstream of the transmembrane protein 238 like(TMEM238L)gene and is encapsulated within a genomic segment characterized by the histone modification H3K27me3.Focused fine-mapping for the SSC12 QTL associated with adenine and hypoxanthine levels narrowed its scope to around 172 kb,encompassing the growth arrest specific 7(GAS7)and myosin heavy chain 13(MYH13)genes.However,the observed QTL effect was not attributed to any missense mutations within the two genes.This pioneering study unveils the genetic variations and candidate genes associated with purine content in livestock,laying a robust foundation for the selective breeding of pig lines with reduced purine base content. 展开更多
关键词 pig purine content genetic architecture GWAS QTL
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Genetic diversity in Morocco'sunflower“Helianthus annuus L.”gene-bank for autumn-early winter plantation conditions:Agro-morpho-physiological screening 认领 引用
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作者 Karim Houmanat Abdelghani Nabloussi +3 位作者 Atman Adiba Achraf Mamassi Hamid Mazouz Mohamed El Fechtali 《Oil Crop Science》 CAS CSCD 2026年第1期9-19,共11页
Despite Morocco's reliance on sunflower as an oilseed crop,little is known about its agronomic performance when sown in autumn or early winter.This knowledge gap is critical,as spring-sown varieties have shown dec... Despite Morocco's reliance on sunflower as an oilseed crop,little is known about its agronomic performance when sown in autumn or early winter.This knowledge gap is critical,as spring-sown varieties have shown declining performance in recent years under intensifying climate stress.Therefore,targeted breeding strategies could discover genotypes suitable for autumn or early winter sowing,with cold tolerance as a key selection criterion.Currently,‘Ichraq'is the only autumn-planted sunflower variety officially registered in Morocco,although efforts to release additional tolerant varieties are underway.This study evaluated 31 genotypes(MGB1to MGB31)selected from various environments under autumn planting conditions and conserved in the Moroccan Gene Bank.These genotypes were planted in early winter at a mountainous site known for its pronounced winter cold.Eighteen Morphological,physiological and agronomic parameters including initial vigor,leaf area,seed yield,oil content etc.,were assessed using both univariate and multivariate statistical approaches.Analysis of variance revealed significant genotypic differences across most traits,indicating substantial genetic variation.Notably,seed oil content ranged from 23.28%(MGB26)to 43.88%(MGB5),and seed yield from1400 kg/ha(MGB7)to 5400 kg/ha(MGB8).Principal component analysis(PCA)identified that the first principal component,accounting for over 24%of the total phenotypic variance,exhibits a strong positive loading of yield-related traits and chlorophyll content,while displaying a pronounced negative loading for oil content variables.This opposing gradient indicates a clear trade-off between vegetative productivity and oil accumulation across the evaluated genotypes.Hierarchical cluster analysis resolved the germplasm into two principal clusters with high within-group similarity,each further partitioned into relatively homogeneous subgroups.Notably,several genotypes outperformed the control variety Ichraq,underscoring their potential for autumn or early winter cultivation.Nonetheless,essential multi-environment trials remain to validate their phenotypic stability and to ascertain their value as genetic resources for sunflower breeding programs in Morocco and other Mediterranean agro-ecosystems. 展开更多
关键词 Sunflower Breeding Genetic diversity early planting Clustering
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Optimization of a self-tuning force control system for the milling process using a dynamic enhanced genetic algorithm 认领 引用
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作者 Yao Li Zhengcai Zhao +3 位作者 Ning Qian Lei Zhang Wenfeng Ding Yucan Fu 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2026年第2期33-43,共11页
When milling structural components with varying axial depths and widths,cutting forces tend to fluctuate,negatively impacting tool life and machining accuracy.To mitigate the force fluctuations and enhance tool longev... When milling structural components with varying axial depths and widths,cutting forces tend to fluctuate,negatively impacting tool life and machining accuracy.To mitigate the force fluctuations and enhance tool longevity,developing a simple,reliable,and easy-to-implement force control system for milling is essential,which is an important step toward advancing intelligent manufacturing.This paper explores the use of genetic algorithms(GA) for powerful optimization capabilities in developing self-tuning milling force controllers.A comprehensive framework for optimizing a fuzzy logic controller using an enhanced GA is specifically designed for the milling process.The optimization integrates the GA with a simulation model,fine-tuning membership functions and optimizing fuzzy rule selection.The enhanced GA incorporates the Integral of Time-weighted Absolute Error(ITAE) as the fitness criterion to improve the robustness and responsiveness of the controller.The optimized fuzzy logic controller is implemented within a computer numerical control system,adjusting feed rates in real-time to control milling forces.The performance of the proposed controller is validated through step and slope milling tests,demonstrating an average control accuracy of 95.52%.Comparative evaluations with other controllers show that the proposed system offers a significant improvement,achieving up to 4.58% better control accuracy in step milling tests. 展开更多
关键词 Optimization Self-tuning Force control system Milling process Genetic algorithm
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Evaluation of safety and immunogenicity of a genetically modified rabies virus for use as an oral vaccine in several non-target species 认领 引用
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作者 Xijun Wang Hong Huo +8 位作者 Lei Shuai Jinying Ge Liyan Peng Jinming Wang Shuang Xiao Weiye Chen Zhiyuan Wen Jinliang Wang Zhigao Bu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第2期814-819,共6页
Oral immunization is an alternative or supplementary approach that can significantly improve dog vaccination coverage,especially for free-roaming dogs.Safe and effective oral rabies vaccines for dogs are still being s... Oral immunization is an alternative or supplementary approach that can significantly improve dog vaccination coverage,especially for free-roaming dogs.Safe and effective oral rabies vaccines for dogs are still being sought.In our previous studies,we generated a genetically modified rabies virus(RABV) ERA strain,rERAG333E,containing a mutation from arginine(Arg,R) to glutamic acid(Glu,E) at residue 333 of the G protein(G333E).Our previous results demonstrated that rERAG333E was safe for adult mice and dogs,and oral vaccination with rERAG333E induced a strong and long-lasting protective immune response in dogs.Here,we further investigated the safety and immunogenicity of rERAG333E in nontarget species,including suckling mice,rhesus monkeys,foxes,raccoon dogs,piglets,goats,and sheep.Suckling mice studies demonstrated that the G333E mutation significantly reduced the virulence of the ERA strain.All of the suckling mice aged 10 days and above survived and showed no apparent signs of disease after intracerebral inoculation with rERAG333E.Animal studies demonstrated that rERAG333E was safe in rhesus monkeys,foxes,raccoon dogs,piglets,goats,and sheep.None of those animals inoculated orally with 10 times the intended field dose of rERAG333E showed abnormal clinical signs before and after the booster immunization with Rabvac 3,an inactivated rabies vaccine.Meanwhile,oral inoculation with rERAG333E induced strong neutralizing antibody(NA) responses to RABV in rhesus monkeys,foxes,raccoon dogs,and piglets.These results demonstrated that rERAG333E has the potential to serve as a safe oral rabies vaccine for dogs. 展开更多
关键词 rabies genetically modified rabies virus oral vaccine
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Global trends in the commercialization of genetically modified crops in 2024 认领 引用
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作者 Haohui Li Xingru Cheng +5 位作者 Liqiang Wang Pei Xie Haiwen Zhang Yadong Yang Tao Liu Youhua Wang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第4期1307-1315,共9页
The ongoing commercialization of genetically modified(GM)crops continues to enhance global grain yields,improve crop quality,and reduce pesticide usage.These technological advancements have effectively propelled agric... The ongoing commercialization of genetically modified(GM)crops continues to enhance global grain yields,improve crop quality,and reduce pesticide usage.These technological advancements have effectively propelled agricultural production systems toward sustainable transformation.Specifically,GM crops address core challenges such as pest infestations,weed proliferation,and arable land constraints,emerging as a pivotal new productive force in agriculture.This study systematically examines the global spatial distribution patterns of GM crops in 2024 and provides an indepth analysis of the driving forces and evolving regional trends,offering critical informational support and strategic guidance for innovation in agricultural science and technology.In 2024,the global GM crop cultivation area reached 209.8 million hectares,a 1.7%year-on-year increase.GM Glycine max(soybean)and Zea mays(maize)dominated the landscape,accounting for 50.0 and 32.5%of the total area,respectively.Among them,maize with stacked traits of insect resistance and herbicide tolerance accounts for 92.5%of GM maize.The share of cultivation in developing countries expanded substantially,with Brazil and Vietnam emerging as regional growth drivers.Policy support and the diffusion of advanced technologies were identified as core driving forces.Concurrently,applications of gene-editing technology accelerated,and several countries approved novel traits such as drought tolerance and disease resistance,marking substantial progress in the commercialization of next-generation GM crops.This research provides multidimensional insights and strategic guidance to support global agricultural biotechnology development,promoting the transition of biotechnology breeding into the‘4.0 era'. 展开更多
关键词 genetically modified crops commercialization global agriculture cultivation area biotechnology
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Efficient regeneration and genetic transformation of fig(Ficus carica)from stem thin cell layer explants 认领 引用
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作者 Xiaojiao Gu Jiajun He +6 位作者 Huaying He Yuan Wang Zhiyi Fan Dan Zhao Kairong Sun Chuanlin Zheng Huiqin Ma 《Horticultural Plant Journal》 SCIE CAS CSCD 2026年第6期1361-1370,共10页
In this study,a rapid,efficient,and stable Agrobacterium-mediated genetic transformation system was successfully developed using stem slices of fig cultivar '117D' as explants.In the Murashige and Skoog(MS) ba... In this study,a rapid,efficient,and stable Agrobacterium-mediated genetic transformation system was successfully developed using stem slices of fig cultivar '117D' as explants.In the Murashige and Skoog(MS) basal medium supplemented with 2 mg·L-1 thidiazuron(TDZ) and 0.05 mg·L-1 1-naphthaleneacetic acid(NAA),the highest shoot-induction rate was 53.33%,while the medium with a hormone ratio of 3 mg·L-1TDZ and 0.05 mg·L-1 NAA achieved a maximum callus-induction rate of 78.89%.The optimal infection parameters for fig callus and stem slices were obtained by infecting thin cell layers(TCLs) with Agrobacterium tumefaciens K599(OD600=0.6) under vacuum for 10 min,and shaking at 120 r·min-1,28℃ for 30 min.Finally,transgenic callus and plants were identified through green fluorescent protein(GFP) screening,β-glucuronidase(GUS) staining,PCR analysis,and Western blot analysis,successfully obtaining positive FcMYB114-overexpressing(FcMYB114-OE) callus and three transgenic plants.Utilizing these FcMYB114 transgenic callus and plants allows us to gain deeper insights into their roles in the growth,development,and metabolism of fig.In the future,we aim to identify new regulatory factors and leverage this efficient transgenic technology to investigate the regulatory mechanisms of key molecules in fig.The advancement of fig regeneration and transgenic systems provides a valuable tool for validating genetic functions in fruit trees and enhancing the agronomic traits of fig. 展开更多
关键词 Ficus carica Tissue culture TCL Agrobacterium Genetic transformation
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Local adaptation sustains genetic differentiation between two varieties of Chinese walnut(Juglans cathayensis)despite extensive hybridization in subtropical China 认领 引用
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作者 Wan Hu Caixia Han +11 位作者 Li Li Chunhua Zang Runzi Li Hong Nie Jianbo Nie Yong Shi Chen Feng Jie Zhang Yixuan Kou Zhiyong Zhang Dengmei Fan Danqi Li 《Plant Diversity》 SCIE CAS CSCD 2026年第4期734-746,共13页
Understanding how natural selection sustains genetic differentiation despite ongoing hybridization remains a central question in evolutionary biology.Here,we integrated range-wide whole-genome resequencing data and ec... Understanding how natural selection sustains genetic differentiation despite ongoing hybridization remains a central question in evolutionary biology.Here,we integrated range-wide whole-genome resequencing data and ecological niche modeling to elucidate the evolutionary history,local adaptation,and hybridization patterns of Juglans cathayensis,a walnut species widely distributed across subtropical China.Our analyses revealed that Chinese walnut comprised two genetic clusters corresponding to its two recognized varieties,which were further subdivided into three lineages:the East lineage,consisting exclusively of J.cathayensis var.formosana individuals;and the West and Admixed lineages,comprising genetically pure and admixed individuals of J.cathayensis var.cathayensis,respectively.The admixed populations formed a hybrid zone in the ecotone between the East and West lineages.Divergence between the East and West lineages dates to the Middle Pliocene,with persistent bidirectional gene flowuntil the mid-Pleistocene,likely driven by long-term local adaptation to niche differences between eastern and western China.Genomic regions of differentiation may result from divergent selection under gene flowand divergent sorting of ancient polymorphisms.Moreover,we identifiedpositively selected genes and environment-associated loci involved in ecological adaptation,underscoring their role in promoting intraspecificdifferentiation.Bayesian genomic cline analysis detected limited introgression of adaptive loci in the hybrid zone,suggesting that natural selection sustains divergence in genomic regions associated with local adaptation,while neutral loci are homogenized through hybridization.Together,these findingsprovide novel insights into the evolutionary mechanisms shaping plant diversity in subtropical China,a region recognized as an evolutionary cradle. 展开更多
关键词 Local adaptation Genetic differentiation Hybrid zone Juglans cathayensis Subtropical China
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Integrating parental breeding value,genetic gain,and gamete contribution for elite family selection in Platycladus orientalis 认领 引用
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作者 Si‑Qian Jiao Meiyu Li +6 位作者 Zhi‑Chao Li Yu‑Tao Bao Hui‑Jin Zhang Xiao‑Lei Yang Yousry Aly El‑Kassaby Shi‑Ping Cheng Jian‑Feng Mao 《Journal of Forestry Research》 SCIE EI CAS CSCD 2026年第3期367-384,共18页
Platycladus orientalis(L.)Franco seed orchards play an important role in sustainable forestry in China but balancing genetic gain and genetic diversity remains a significant challenge.Two key factors influence the suc... Platycladus orientalis(L.)Franco seed orchards play an important role in sustainable forestry in China but balancing genetic gain and genetic diversity remains a significant challenge.Two key factors influence the success of seed orchards:parental breeding value and gamete contribution,as they determine both the genetic gain and diversity of the seed crops produced.This study aimed to optimize breeding strategies by analyzing parental breeding value,gamete contribution,and genetic gain across two growth periods(89 families in 2008 and 52 families in 2021).We evaluated height,diameter at breast height,and stem volume of progeny in a primary seed orchard,uncovering significant genetic variation among families.Interestingly,no correlation was found between growth traits and gamete contribution,indicating their independence.Using comprehensive scoring and PCA-biplot analysis,we consistently identified several elite families with superior growth performance in both years.We propose an optimal breeding strategy that combines 30%selective harvesting and 50%selective thinning to effectively balance genetic gain and genetic diversity,addressing a critical goal in tree improvement programs.The selected families and optimized strategy provide a scalable framework not only for P.orientalis but also for other conifer species globally,enhancing both productivity and genetic diversity in afforestation efforts. 展开更多
关键词 Platycladus orientalis Progeny test Comprehensive evaluation Genetic gain Breeding strategy
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Unraveling the bidirectional association between mental disorders and prostatitis:insights from a genetic perspective 认领 引用
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作者 Guancan Liang Jian Pan +5 位作者 Ruixiang Dai Ziyi Lin Xunbao Wang Teng Hou Zhicheng Luo Xiaoming Wang 《The Canadian Journal of Urology》 SCIE 2026年第3期563-571,共9页
Background:The causal link betweenmental illness and prostatitis remains inconclusive,largely due to heterogeneity and potential confounders.This study explored the causal link between mental illness and prostatitis i... Background:The causal link betweenmental illness and prostatitis remains inconclusive,largely due to heterogeneity and potential confounders.This study explored the causal link between mental illness and prostatitis in men using Mendelian randomization(MR),and offered recommendations for enhancing future research.Methods:Publicly accessible genome-wide association study(GWAS)data were accessed via the IEU OpenGWAS platform and FinnGen database for this research.The inverse variance weighted(IVW)approach served as the primaryMendelian randomization analysis,while MR-Egger,weighted median,weighted mode,and simple mode methods were additionally applied to evaluate potential relationships between prostatitis and four psychiatric disorders(schizophrenia,depression,bipolar disorder,and anxiety).Results:The analysis indicated a signicant causal association between depression and prostatitis(OR=1766.294,p=0.01),whereas no evidence of a causal relationship was observed for schizophrenia,bipolar disorder,or anxiety with prostatitis(p>0.05).In the reverse-direction MR analysis,prostatitis showed no evidence of a causal effect on psychiatric disorders.Further sensitivity analyses did not reveal pleiotropy or heterogeneity,and leave-one-out analyses indicated that the overall results were not signicantly affected by any single instrumental variable.Sensitivity analyses provided no indication of pleiotropy or heterogeneity,and leave-one-out testing suggested that the overall results remained stable regardless of the exclusion of any single instrumental variable.Conclusion:The present study provides genetic evidence that depression may increase the risk of prostatitis,highlighting the need for early preventive strategies.Additional studies are needed to clarify the mechanisms connecting depression and prostatitis in men. 展开更多
关键词 Prostatitis mental disorders mendelian randomization genetics causal effect
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Genetic dissection of an extremely early heading mutant in Aegilops tauschii 认领 引用
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作者 Shengke Li Laibin Zhao +14 位作者 Xiaolu Wang Ke Zheng Chaolan Fan Lin Huang Ming Hao Xuejiao Chen Xue Chen Lianquan Zhang Zhongwei Yuan Bo Jiang Qingqi Fan Cheng Liu Takao Komastuda Dengcai Liu Shunzong Ning 《The Crop Journal》 SCIE CSCD 2026年第3期1079-1084,共6页
The heading date is an important trait that affects crop adaptability and significantly affects crop yield.The discovery and functional understanding of genes related to heading are crucial for wheat breeding in vario... The heading date is an important trait that affects crop adaptability and significantly affects crop yield.The discovery and functional understanding of genes related to heading are crucial for wheat breeding in various environments.Aegilops tauschii,the D-genome donor of wheat,serves as an important model for exploring genetic regulatory mechanisms in common wheat.In this study,extremely early heading mutants were identified after treatment with 0.4%ethyl methanesulfonate(EMS)and compared with the wild type.Genetic analysis revealed that a single recessive gene was responsible for early heading,and gene mapping revealed a mutation in a 10.3-Mb region on the short arm of chromosome 6D.Bulked segregant exon capture sequencing revealed a missense mutation within the highly conserved nucleotide-binding domain of AET6 Gv20469900,which encodes a protein phosphatase 2C(PP2C).This gene was in the mapping interval and associated with early heading.Transcriptomic profiling and quantitative validation analyses suggest that PP2C likely modulates vernalization pathways,thereby affecting heading date.This work provides a novel genetic resource for breeding early heading wheat cultivars. 展开更多
关键词 Alien genetic resource Heading date PP2C Aegilops tauschii
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Genetic model of intraplate volcanic high-temperature geothermal system in Yanggao-Tianzhen Basin,Datong 认领 引用
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作者 Shaochuan Sun Xinwei Wang +3 位作者 Yanxin Wang Xiang Mao Jian Liu Lu Luo 《Energy Geoscience》 EI CAS CSCD 2026年第3期112-127,共16页
The Yanggao-Tianzhen Basin comprises three stratigraphic sequences from top to bottom,namely the Quaternary,Neogene-Paleogene,and Archean.The Quaternary serves as the regional caprock,and heat transfer within the basi... The Yanggao-Tianzhen Basin comprises three stratigraphic sequences from top to bottom,namely the Quaternary,Neogene-Paleogene,and Archean.The Quaternary serves as the regional caprock,and heat transfer within the basin occurs primarily via thermal conduction,with a higher geothermal gradient in the eastern part than in the west.Heat sources are distributed in the northern and eastern regions at a burial depth ranging from 4000 to 8000 m.Shallow-buried heat sources dominate the western part,while the eastern part features distinct magmatic channels connecting to shallow strata.The main fault system in the basin,trending NE-SW,provides pathways for deep heat sources.Unerupted concealed volcanic clusters are mainly distributed in the Yanggao area(central basin),consistent with the basin's structural strike;in contrast,concealed volcanic craters in the Tianzhen area are scattered in isolation,with visible magmatic channels.The hydrochemical type of shallow geothermal water is HCO3-Ca·Mg,while that of deep geothermal water is HCO3·Cl·SO4-Na.Geothermal water originates from atmospheric precipitation and exhibits poor circulation and renewal conditions.The formation and distribution of intraplate volcanic high-temperature geothermal resources are controlled by the combination of concealed volcanic distribution and deep/large faults.By characterizing the spatial structure of unerupted concealed volcanoes as well as the dynamic processes of heat conduction in the fault system and hydrothermal circulation,we propose a trinity genetic model of"magmatic heat source-structural conduction-shallow local thermal anomaly".Moreover,it is believed that the intraplate hightemperature geothermal system is activated and formed due to the long-range effect of plate subduction and collision caused by the"modification of supergene structures by deep processes". 展开更多
关键词 Datong volcanic group Yanggao-Tianzhen Basin Intraplate Concealed volcano Genetic model
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