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Immunogenicity and Safety of vYF,a Yellow Fever Vaccine-A Phase 2 Trial 认领 引用
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作者 Emmanuel Feroldi 《四川生理科学杂志》 2026年第5期1034-1034,共1页
Background:A next-generation,live-attenuated yellow fever vaccine,vYF,was developed in Vero cells to improve vaccine supply and availability.The safety of and immune response to vYF as compared with those of the licen... Background:A next-generation,live-attenuated yellow fever vaccine,vYF,was developed in Vero cells to improve vaccine supply and availability.The safety of and immune response to vYF as compared with those of the licensed yellow fever vaccine,YF-VAX,are unclear.Methods:In this year 1 interim analysis of a phase 2,observer-blinded,randomized,active-controlled trial,we randomly assigned healthy adults 18 to 60 years of age in a 2:1 ratio to receive vYF or YF-VAX as a single vaccine injection on day 1.Neutralizing antibody titers were measured on day 29,month 6,and year 1. 展开更多
关键词 randomly assigned healthy adults vyf yellow fever vaccineyf vaxare yellow fever safety yellow fever vaccine vero cells immunogenicity
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Immunogenicity and efficacy of an LNP-mRNA prepared from African swine fever virus K205R 认领 引用 被引量：2
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作者 Chuanwen Tian Yingnan Liu +6 位作者 Dongdong Di Zhenhua Xie Yao Li Rongrong Wang Jie Li Jingyi Liu Hongjun Chen 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2025年第10期4026-4033,共8页
African swine fever(ASF),caused by African swine fever virus(ASFV),is a highly contagious swine disease that has spread globally.Effective control strategies are not yet available.In this study,we prepared K205R mRNA,... African swine fever(ASF),caused by African swine fever virus(ASFV),is a highly contagious swine disease that has spread globally.Effective control strategies are not yet available.In this study,we prepared K205R mRNA,which was then formulated using Lipid Nanoparticle(LNP).The resulting K205R mRNA-LNP showed a particle size of approximately 86.27 nm and an mRNA encapsulation efficiency of 96.24%.Efficient expression of the K205R protein was confirmed in both HEK293T and PK15 cells.We further evaluated the immunogenicity of K205R mRNA-LNP in mice and pigs.All immunized animals developed significantly higher levels of IgG antibodies against K205R compared to the control group in the first week after the second immunization,with antibody titers reaching up to 105.Challenge experiments showed that K205R mRNA delayed the time of death.Our results suggested the successful implementation of the mRNA platform in the preparation and application of ASFV mRNA. 展开更多
关键词 ASFV K205R mRNA immunogenicity
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Folic acid ameliorates the immunogenicity of PEGylated liposomes 认领 引用
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作者 Xiying Wu Anze Liu +2 位作者 Yuzhong Yan Ying Lu Huan Wang 《Chinese Chemical Letters》 SCIE CAS CSCD 2025年第6期310-313,共4页
As PEGylated liposomes have witnessed remarkable advancements in drug delivery,their immunogenicity has emerged as a notable challenge.In this study,we discovered that a simple pre-injection of folic acid(FA)effective... As PEGylated liposomes have witnessed remarkable advancements in drug delivery,their immunogenicity has emerged as a notable challenge.In this study,we discovered that a simple pre-injection of folic acid(FA)effectively mitigated the immunogenicity of PEGylated liposomes and enhanced their in vivo performance by tolerating splenic marginal zone B cells.FA specifically inhibited the internalization of PEGylated liposomes by splenic marginal zone B cells,thereby reducing splenic lymphocyte proliferation and specific IgM secretion.This modulation alleviated Ig M-mediated accelerated blood clearance and adverse accumulation of the PEGylated liposomes in the skin.These findings provide new insights into the immunomodulatory effects of FA and promising avenues to enhance the efficacy and safety of PEGylated liposomal nanomedicines. 展开更多
关键词 Folic acid Pegylated liposomes Immunogenicity Marginal zone B cells Accelerated blood clearance
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SITA: Predicting site-specific immunogenicity for therapeutic antibodies 认领 引用 被引量：1
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作者 Yewei Cun Hao Ding +7 位作者 Tiantian Mao Yuan Wang Caicui Wang Jiajun Li Zihao Li Mengdie Hu Zhiwei Cao Tianyi Qiu 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2025年第6期1378-1389,共12页
Antibody humanization is critical to reduce immunogenicity and enhance efficacy in the preclinical phase of the development of therapeutic antibodies originated from animal models.Computational suggestions have long b... Antibody humanization is critical to reduce immunogenicity and enhance efficacy in the preclinical phase of the development of therapeutic antibodies originated from animal models.Computational suggestions have long been desired,but available tools focused on immunogenicity calculation of whole antibody sequences and sequence segments,missing the individual residue sites.This study introduces Site-specific Immunogenicity for Therapeutic Antibody(SITA),a novel computational framework that predicts B-cell immunogenicity score for not only the overall antibody,but also individual residues,based on a comprehensive set of amino acid descriptors characterizing physicochemical and spatial features for antibody structures.A transfer-learning-inspired framework was purposely adopted to overcome the scarcity of Antibody-Antibody structural complexes.On an independent testing dataset derived from 13 Antibody-Antibody structural complexes,SITA successfully predicted the epitope sites for Antibody-Antibody structures with a receiver operating characteristic(ROC)-area unver the ROC curve(AUC)of 0.85 and a precision-recall(PR)-AUC of 0.305 at the residue level.Furthermore,the SITA score can significantly distinguish immunogenicity levels of whole human antibodies,therapeutic antibodies and non-human-derived antibodies.More importantly,analysis of an additional 25 thera-peutic antibodies revealed that over 70%of them were detected with decreased immunogenicity after modification compared to their parent variants.Among these,nearly 66%antibodies successfully iden-tified actual modification sites from the top five sites with the highest SITA scores,suggesting the ability of SITA scores for guide the humanization of antibody.Overall,these findings highlight the potential of SITA in optimizing immunogenicity assessments during the process of therapeutic antibody design. 展开更多
关键词 Machine learning Therapeuticantibody Immunogenicity Humanization
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Diphtheria immunogenicity following the administration of pentavalent DTwP-HB-Hib vaccine at 4-and 8-week intervals:A randomized controlled trial 认领 引用
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作者 Ariesti Karmila Muhammad Yudi Rakhmadi +1 位作者 Moretta Damayanti Yulia Iriani 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2025年第9期385-390,共6页
Objective:To evaluate the effectiveness of DTwP-HB-Hib vaccination dosing intervals at eight weeks versus four weeks on the immunogenicity of the diphtheria component.Methods:This is a randomized,open-label,parallel,c... Objective:To evaluate the effectiveness of DTwP-HB-Hib vaccination dosing intervals at eight weeks versus four weeks on the immunogenicity of the diphtheria component.Methods:This is a randomized,open-label,parallel,controlled trial on healthy two-month-old infants who had not received DTwP-HB-Hib vaccinations.The infants received three doses of the vaccine either at an eight-week or four-week interval.The anti-diphtheria toxoid IgG antibody levels before and after three doses of the vaccination were measured using ELISA.Results:Eighty infants were enrolled in this study,with 64 fulfilling the study requirements and randomized into two groups.All study participants exhibited uncertain protection against diphtheria antibodies(0.01-0.99 IU/mL)at the beginning of the study with an average of(0.023±0.009)IU/mL and(0.026±0.009)IU/mL in eight weeks and four weeks groups.Following three doses of the vaccination,the antibody level rose to an average of(1.320±0.234)IU/mL in the eight-week group and(1.307±0.186)IU/mL in the four-week group,with no statistically significant difference in the antibody levels observed(P=0.814).Conclusions:DTwP-HB-Hib vaccinations can be administered at an eight-week or four-week interval,as they do not significantly affect the level of anti-diphtheria IgG antibodies. 展开更多
关键词 Anti-diphtheria toxoid IgG antibody DTwP-HBHib vaccine Dosing interval Immunogenicity
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Rabies Virus Neutralizing Activity,Safety,and Immunogenicity of Recombinant Human Rabies Antibody Compared with Human Rabies Immunoglobulin in Healthy Adults 认领 引用 被引量：4
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作者 ZHANG Jun Nan MENG Ya Juan +16 位作者 BAI Yun Hua LI Yu Feng YANG Li Qing SHI Nian Min HAN Hui Xia GAO Jian ZHU Li Juan LI Shu Ping ZHANG Jing ZHAO Qin Hua WANG Xiu Qin WEI Jing Shuang REN Le Min CAO Chen Hua CHEN Chen ZHAO Wei LI Li 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2022年第9期782-791,共10页
Objective Preliminary assessment of rabies virus neutralizing activity,safety and immunogenicity of a recombinant human rabies antibody(NM57)compared with human rabies immunoglobulin(HRIG)in Chinese healthy adults.Met... Objective Preliminary assessment of rabies virus neutralizing activity,safety and immunogenicity of a recombinant human rabies antibody(NM57)compared with human rabies immunoglobulin(HRIG)in Chinese healthy adults.Methods Subjects were randomly(1:1:1)allocated to Groups A(20 IU/kg NM57),B(40 IU/kg NM57),or C(20 IU/kg HRIG).One injection was given on the day of enrollment.Blood samples were collected on days-7 to 0(pre-injection),3,7,14,28,and 42.Adverse events(AEs)and serious AEs(SAEs)were recorded over a period of 42 days after injection.Results All 60 subjects developed detectable rabies virus neutralizing antibodies(RVNAs)(>0.05 IU/mL)on days 3,7,14,28,and 42.The RVNA levels peaked on day 3 in all three groups,with a geometric mean concentration(GMC)of 0.2139 IU/mL in Group A,0.3660 IU/mL in Group B,and0.1994 IU/mL in Group C.At each follow-up point,the GMC in Group B was significantly higher than that in Groups A and C.The areas under the antibody concentration curve over 0-14 days and 0-42 days in Group B were significantly larger than those in Groups A and C.Fifteen AEs were reported.Except for one grade 2 myalgia in Group C,the other 14 were all grade 1.No SAEs were observed.Conclusion The rabies virus neutralizing activity of 40 IU/kg NM57 was superior to that of 20 IU/kg NM57 and 20 IU/kg HRIG,and the rabies virus neutralizing activity of 20 IU/kg NM57 and 20 IU/kg HRIG were similar.Safety was comparable between NM57 and HRIG. 展开更多
关键词 Recombinant human rabies antibody NM57 Human rabies immunoglobulin Rabies virus neutralizing activity Safety Immunogenicity
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Expression of Outer Capsid Protein VP5 of Grass Carp Reovirus in E.coli and Analysis of its Immunogenicity 认领 引用 被引量：5
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作者 Lan-lan ZHANG Jin-yu SHEN +3 位作者 Cheng-feng LEI Chao FAN Gui-jie HAO Qin FANG 《Virologica Sinica》 CAS 2009年第6期545-551,共7页
Grass carp reovirus (GCRV) is a tentative member of the Aquareovirus genus in the family Reoviridae. The mature virion comprises 11 dsRNA genomes enclosed by two concentric icosahedral proteins shells that is comprise... Grass carp reovirus (GCRV) is a tentative member of the Aquareovirus genus in the family Reoviridae. The mature virion comprises 11 dsRNA genomes enclosed by two concentric icosahedral proteins shells that is comprised of five core proteins and two outer capsid proteins. The genome sequence and 3D structure demonstrate there is a higher level of sequence homology in structural proteins between GCRV and mammalian orthoreoviruses (MRV) compared to other members of the family. To understand the pathogenesis of GCRV infection, the outer capsid protein VP5, a homology of the μ1 protein of MRV, was expressed in E.coli. It was found that the recombinant VP5 was highly expressed, and the expressed His-tag fusion protein was involved in the formation of the inclusion body. Additionally, specific anti-VP5 serum was prepared from purified protein and western blot demonstrated that the expressed protein was able to bind immunologically to rabbit anti GCRV particle serum and the immunogenicity was determined by ELISA assay. Additional experiments in investigating the functional properties of VP5 will further elucidate the role of the GCRV outer capsid protein VP5 during entry into host cells, and its interaction among viral proteins and host cells during the infection process. 展开更多
关键词 Grass carp reovirus （GCRV） Outer capsid protein VP5 Expression in E.coli Immunogenicity
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Expression of Helicobacter pyloriAlpA protein and its immunogenicity 认领 引用 被引量：6
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作者 JingXue YangBai +4 位作者 YeChen Ji-DeWang Zhao-ShanZhang Ya-LiZhang Dian-YuanZhou 《World Journal of Gastroenterology》 SCIE CAS 2005年第15期2260-2263,共4页
AIM:To construct a recombinant strain which expresses adhesin AlpA of Helicobacter pylori (H pylori) and to study the immunogenicity of adhesin AlpA. METHODS: Gene Ab, which was amplified from H pylori chromosomal DNA... AIM:To construct a recombinant strain which expresses adhesin AlpA of Helicobacter pylori (H pylori) and to study the immunogenicity of adhesin AlpA. METHODS: Gene Ab, which was amplified from H pylori chromosomal DNA by PCR technique, was sequenced and the biological information was analyzed, and inserted into the Nco I and Not I restriction fragments of the expression vector pET-22b(+) using T4 DNA ligase. The resulting plasmid pET-AlpA was transformed into competent E.coli BL21(DE3) cells using ampicillin resistance for selection. Recombinant strains were incubated in 5 mL LB with 100 μg/mL ampicillin overnight at 37 ℃. Sonication of BL21(DE3)pET-22b(+)/AlpA was analyzed by Western blot to detect AlpA immunogenicity. RESULTS: The gene encoding AlpA protein was amplified by PCR with chromosomal DNA of H pylori Sydney strain (SS1) as templates. It revealed that AlpA DNA fragment amplified by PCR had approximately 1 500 nucleotides, compatible with the previous reports. The recombinant plasmid pET-22b(+)/AB was successfully constructed. DNA sequencing showed one open reading frame with the length of 588 bp. It encoded seven conservative regions that showed good antigenicity and hydrophobicity by Parker and Welling method. Furthermore, INTERNET EXPASY, NNPREDICT and ISREC predicted that it was a porin-like structure consisting of β-pleated sheets that were embedded in the outer membrane. BLAST analyzed 836 767 protein sequences and found that the similar sequences were all belonging to H pylori OMP sequences. SDS-PAGE and scan analysis showed that the molecular weight of AB was 22.5 ku and recombinant protein amounted to 29% of the total bacterial protein, among which dissolved expression amounted to 21.9% of sonicated supernatant. The rAB purity amounted to 96% through affinity chromatography. Western blot analysis of rAB confirmed that it could be specially recognized by serum form rabbit immunized with AlpA and H pylori infected. CONCLUSION: Adhesin AlpA recombinant protein may be a potential vaccine for control and treatment of H pylori infection. 展开更多
关键词 H pylori Immunogenicity
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Investigation of bone reconstruction using an attenuated immunogenicity xenogenic composite scaffold fabricated by 3D printing 认领 引用 被引量：3
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作者 Qiongxi Pan Chenyuan Gao +8 位作者 Yingying Wang Yili Wang Cong Mao Quan Wang Sophia N.Economidou Dennis Douroumis Feng Wen Lay Poh Tan Huaqiong Li 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2020年第4期396-409,共14页
Bone is known to have a natural function to heal itself.However,if the bone damage is beyond a critical degree,intervention such as bone grafting may be imperative.In this work,the fabrication of a novel bone scaffold... Bone is known to have a natural function to heal itself.However,if the bone damage is beyond a critical degree,intervention such as bone grafting may be imperative.In this work,the fabrication of a novel bone scaffold composed of natural bone components and polycaprolactone(PCL)using 3D printing is put forward.α1,3-galactosyltransferase deficient pigs were used as the donor source of a xenograft.Decellularized porcine bone(DCB)with attenuated immunogenicity was used as the natural component of the scaffold with the aim to promote bone regeneration.The 3D printed DCB-PCL scaffolds combined essential advantages such as uniformity of the interconnected macropores and high porosity and enhanced compressive strength.The biological properties of the DCB-PCL scaffolds were evaluated by studying cell adhesion,viability,alkaline phosphatase activity and osteogenic gene expression of human bone marrow-derived mesenchymal stem cells.The in vitro results demonstrated that the DCB-PCL scaffolds exhibit an enhanced performance in promoting bone differentiation,which is correlated to the DCB content.Furthermore,critical-sized cranial rat defects were used to assess the effect of DCB-PCL scaffolds on bone regeneration in vivo.The results confirm that in comparison with PCL scaffolds,the DCB-PCL scaffolds can significantly improve new bone formation in cranial defects.Thus,the proposed 3D printed DCB-PCL scaffolds emerge as a promising regeneration alternative in the clinical treatment of large bone defects. 展开更多
关键词 Polycaprolactone 3D printing Decellularized porcine bone Cranial bone regeneration Attenuated immunogenicity
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Low immunogenicity of endothelial derivatives from rat embryonic stem cell-like cells 认领 引用 被引量：2
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作者 Juliane Ladhoff Michael Bader +4 位作者 Sabine Brosel Elke Effenberger Dirk Westermann Hans-Dieter Volk Martina Seifert 《Cell Research》 SCIE CAS 2009年第4期507-518,共12页
Embryonic stem cells （ESC） are suggested to be immune-privileged, but they carry the risk of uncontrolled expansion and malignancy. Upon differentiation they lose their tumor-forming capacity, but they become immuno... Embryonic stem cells （ESC） are suggested to be immune-privileged, but they carry the risk of uncontrolled expansion and malignancy. Upon differentiation they lose their tumor-forming capacity, but they become immunogenic by the expression of a normal set of MHC molecules. This immunogenicity might trigger rejection after application in regenerative therapies. In this study MHC expression of and immune responses to endothelial derivatives of rat embryonic stem cell-like cells （RESC） under inflammatory conditions were determined in comparison to primary rat aortic endothelial cells （ECs）. Cellular as well as humoral allo-recognition was analyzed in vitro. In addition, im- mune reactions in vivo were assessed by allo-antibody production and determination of interferon-y （IFNy）-secreting alio-reactive T cells. RESC derivatives expressed low but significant levels of MHC class I, and no MHC class II. In response to IFNy MHC class I expression was enhanced, while class II transactivator induction failed completely in these cells; MHC class II expression remained consistently absent. Functionally, the RESC derivatives showed a reduced allo-stimulatory capacity, protection against humoral allo-recognition in vitro and a slightly diminished sus- ceptibility to cytotoxic T cell lysis. Furthermore, in vivo experiments demonstrated that these ceils do not trigger host immune reactions, characterized by no allo-antibody production and no induction of allo-reactive memory T cells. Our results show that endothelial derivatives of RESC have a distinctive reduced immunogenic potency even under inflammatory conditions. 展开更多
关键词 stem cells cell differentiation endothelial cells MHC CIITA immunogenicity
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Immunogenicity Analysis of Prokaryotic Expression Products of Kaposi's Sarcoma Associated Herpesvirus orf65 认领 引用 被引量：6
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作者 Bi-shi FU Bao-lin LI Lin-ding WANG 《Virologica Sinica》 CAS 2008年第3期196-202,共7页
To purify the protein encoding the small capsid protein （SCP） of KSHV and analyze its immunogenicity, the carboxyl terminus of orf65 of Kaposi＇s sarcoma associated-herpesvirus （KSHV） was expressed in a prokaryoti... To purify the protein encoding the small capsid protein （SCP） of KSHV and analyze its immunogenicity, the carboxyl terminus of orf65 of Kaposi＇s sarcoma associated-herpesvirus （KSHV） was expressed in a prokaryotic expression system. The expression of recombinant E.coli containing pQE-80L-orf65 was induced by isopropyl-13-D-thiogalactopyranoside （IPTG） and the fusion protein was purified by chromatography. The expressed protein and its purified product were identified by sodium dodecyl sulfate- polyacrylamide gel electrophoresis （SDS-PAGE） and showed that 9 kDa was the expected size of the purified orf65 protein. The antiserum was produced in rabbit which was immunized by purified orf65 protein. An ELISA assay was established to analyze the immunogenicity of the purified orf65 protein. The ELISA analysis demonstrated that orf65 protein has strong immune activity, and the immune activity of polyclonal antibody against orf65 was more than 4 fold higher than that in the serum of the non-immunized rabbit. These results demonstrate that purified orf65 protein has very strong immunogenicity and can be used in screening KSHV infection in the general population using ELISA. 展开更多
关键词 Kaposi＇s sarcoma-associated herpesvirus （KSHV） orf65 Chromatography Immunogenicity
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Immunogenicity of recombinant hepatitis B virus vaccine in patients with and without chronic hepatitis C virus infection:A case-control study 认领 引用 被引量：2
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作者 Naser Ebrahimi Daryani Mohsen Nassiri-Toosi +1 位作者 Armin Rashidi Iman Khodarahmi 《World Journal of Gastroenterology》 SCIE CAS 2007年第2期294-298,共5页
AIM： To compare the response of standard hepatitis B virus （HBV） vaccination between patients with chronic hepatitis C virus （HCV） infection and healthy individuals. METHODS： This is a prospective case-control s... AIM： To compare the response of standard hepatitis B virus （HBV） vaccination between patients with chronic hepatitis C virus （HCV） infection and healthy individuals. METHODS： This is a prospective case-control study. A total of 38 patients with chronic HCV infection and 40 healthy controls were included. Vaccination was performed by injection of 20μg recombinant HBsAg into the deltoid muscle at mo 0,1 and 6. Anti-HBs concentration was determined 3 mo after the last dose and compared between the two groups. The response pattern was characterized as （1） high-response when the anti-HBs antibody titer was 〉 100 IU/L, （2） low-response when the titer was 10-100 IU/L. and （3） no-response when the titer was 〈 10 IU/L. RESULTS： In the patient group, there were 10/38 （26.3%） non-responders, 8/38 （21.1%） Iow-responders and 20/38 （52.6%） high-responders. The corresponding values in the control group were 2/40 （5.0%）, 7/40 （17.5%） and 31/40 （77.5%）, respectively. The response pattern was statistically different between the two groups. In multivariate analysis, smoking was a significant confounder, while HCV infection lost its significant correlation with lower antibody response. CONCLUSION： Patients with chronic HCV infection tend to respond weakly to HBV vaccination compared to healthy individuals, though this correlation is not independent according to multivariate analysis. 展开更多
关键词 Immunogenicity Hepatitis B Vaccine Hepatitis C Antibody response
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Generation and Immunogenicity of a Recombinant Adenovirus Co-Expressing the E2 Protein of Classical Swine Fever Virus and the GP5 Protein of Porcine Reproduction and Respiratory Syndrome Virus 认领 引用 被引量：3
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作者 LI Hong-yu SUN Yuan +5 位作者 ZHANG Xing-juan CHANG Tian-ming WANG Xiang-peng HE Fan HUANG Jun-hua QIU Hua-ji 《Agricultural Sciences in China》 CSCD 2011年第11期1781-1791,共11页
Classical swine fever(CSF)and porcine reproduction and respiratory syndrome(PRRS)are both economically important,highly contagious diseases of swine worldwide.To develop an effective vaccine to control these two disea... Classical swine fever(CSF)and porcine reproduction and respiratory syndrome(PRRS)are both economically important,highly contagious diseases of swine worldwide.To develop an effective vaccine to control these two diseases,we constructed a recombinant adenovirus rAdV-GP52AE2,using a replication-defective human adenovirus serotype 5 as a delivery vector,to co-express the GP5 protein of highly pathogenic porcine reproduction and respiratory syndrome virus(PRRSV)and the E2 protein of classical swine fever virus(CSFV).Foot-and-mouth disease virus(FMDV)2A peptide was used as a linker between the GP5 and E2 proteins to allow automatic self-cleavage of the polyprotein.The GP5 and E2 genes were expressed as demonstrated by immunofluorescence assay and Western blotting.Immunization of mice resulted in a CSFV-neutralizing antibody titer of 1:128 and a PRRSV-neutralizing antibody titer of 1:16.The lymphoproliferative responses were detected by Cell Counting Kit-8 assay and the stimulation index of CFSV-specific and PRRSV-specific lymphocytes in the rAdV-GP52AE2 group was significantly higher than that in the negative control group.The results show that rAdV-GP52AE2 can induce both effective humoral and cell-mediated immune responses in mice.The protective efficacy of the recombinant virus against CSF was evaluated in immunized rabbits,which were protected from fever induced by challenge with C-strain.Our study provides supporting evidence for the use of FMDV 2A to develop a bivalent genetically-engineered vaccine. 展开更多
关键词 porcine reproductive and respiratory syndrome virus classical swine fever virus recombinant adenovirus,immunogenicity
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The truncated form of fagellin (tFlic) provides the 2dCap subunit vaccine with better immunogenicity and protective efects in mice 认领 引用 被引量：2
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作者 Ying Lu Zehui Liu +3 位作者 Yingxiang Li Zhuofan Deng Weihuan Fang Fang He 《Animal Diseases》 CAS 2022年第2期118-126,共9页
Porcine circovirus type 2(PCV2)is the main causative agent of porcine circovirus-associated diseases,and it causes substantial economic losses in the swine industry each year.It is crucial to develop an efective vacci... Porcine circovirus type 2(PCV2)is the main causative agent of porcine circovirus-associated diseases,and it causes substantial economic losses in the swine industry each year.It is crucial to develop an efective vaccine against the circulating strain PCV2d,which is prone to substantial degrees of mutation.In this study,a truncated form of fagellin(tFlic:85-111 aa)was inserted into the C-terminal sequence of 2dCap,and Western blotting results showed that recombinant Cap-tFlic VLPs were successfully expressed.Transmission electron microscopy(TEM)and dynamic light scattering(DLS)data indicated that purifed recombinant Cap-tFlic fusion proteins existed in the form of polymers and that tFlic could not afect the formation and internalization of VLPs.Integrated Cap-tFlic VLPs induced the expression of antigen presentation-related factors(MHC-II and CD86)by bone marrow-derived dendritic cells(BM-DCs),and the expression of TLR5-related factors(TNF-α)was dramatically elevated.Mice intramuscularly immunized with Cap-tFlic VLPs exhibited signifcantly higher levels of Cap-specifc antibodies and neutralizing antibodies than mice immunized with wild-type Cap VLPs.The data obtained in the current study indicate that Cap-tFlic may be a candidate for a subunit vaccine against PCV2 in the future. 展开更多
关键词 Porcine circovirus type 2(PCV2) Virus-like particles(VLPs) Truncated form of fagellin(tFlic) Immunogenicity
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Immunogenicity of multi-epitope-based vaccine candidates administered with the adjuvant Gp96 against rabies 认领 引用 被引量：1
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作者 Yange Niu Ye Liu +5 位作者 Limin Yang Hongren Qu Jingyi Zhao Rongliang Hu Jing Li Wenjun Liu 《Virologica Sinica》 SCIE CAS CSCD 2016年第2期168-175,共8页
Rabies, a zoonotic disease, causes > 55,000 human deaths globally and results in at least 500 million dollars in losses every year. The currently available rabies vaccines are mainly inactivated and attenuated vacc... Rabies, a zoonotic disease, causes > 55,000 human deaths globally and results in at least 500 million dollars in losses every year. The currently available rabies vaccines are mainly inactivated and attenuated vaccines, which have been linked with clinical diseases in animals. Thus, a rabies vaccine with high safety and efficacy is urgently needed. Peptide vaccines are known for their low cost, simple production procedures and high safety. Therefore, in this study, we examined the efficacy of multi-epitope-based vaccine candidates against rabies virus. The ability of various peptides to induce epitope-specific responses was examined, and the two peptides that possessed the highest antigenicity and conservation, i.e., AR16 and h PAB, were coated with adjuvant canineGp96 and used to prepare vaccines. The peptides were prepared as an emulsion of oil in water(O/W) to create three batches of bivalent vaccine products. The vaccine candidates possessed high safety. Virus neutralizing antibodies were detected on the day 14 after the first immunization in mice and beagles, reaching 5–6 IU/m L in mice and 7–9 IU/m L in beagles by day 28. The protective efficacy of the vaccine candidates was about 70%–80% in mice challenged by a virulent strain of rabies virus. Thus, a novel multi-epitope-based rabies vaccine with Gp96 as an adjuvant was developed and validated in mice and dogs. Our results suggest that synthetic peptides hold promise for the development of novel vaccines against rabies. 展开更多
关键词 rabies virus multi-epitope-based vaccine immunogenicity evaluation Gp96
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Immunogenicity and protective efficacy of Vibrio harveyi pcFlaA DNA vaccine in Epinephelus awoara 认领 引用 被引量：2
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作者 覃映雪 苏永全 +1 位作者 王世峰 鄢庆枇 《Chinese Journal of Oceanology and Limnology》 2009年第4期769-774,共6页
The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1（＋） （designated as pcFlaA）. Ninety grouper （Epinephelus awoar... The FlaA gene from Vibrio harveyi marker, was cloned into the eukaryotic expression with a short nucleotide sequence encoding the Flag vector pcDNA3.1（＋） （designated as pcFlaA）. Ninety grouper （Epinephelus awoara） were separated into three equal size groups. An experimental group was immunized with pcFlaA, Control I group was immunized with the vector pcDNA3.1（＋）, and Control 1I group was immunized with PBS. The expression of pcFlaA mRNA and protein was examined using reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry. We also evaluated the immunogenicity and protective efficacy of pcFlaA against V. harveyi by measuring the lymphocyte proliferation response and serum levels of specific antibody and conducting a bacterial challenge test. We successfully transfected the fish muscle with pcFlaA. The pcFlaA mRNA and protein was expressed in the muscle cells for up to one month following injection. The proliferation response of lymphocytes in fish immunized with pcFlaA was significantly higher than in control group II. Furthermore, the immunized fish generated specific antibody. The vaccination also resulted in significantly higher survival during the bacterial challenge test. 展开更多
关键词 Vibrio harveyi DNA vaccination immunogenicity protective efficacy
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Immunogenicity and protective role of antigenic regions from five outer membrane proteins of Flavobacterium columnare in grass carp Ctenopharyngodon idella 认领 引用 被引量：3
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作者 罗璋 刘志新 +3 位作者 付建平 张秋胜 黄贝 聂品 《Chinese Journal of Oceanology and Limnology》 CSCD 2016年第6期1247-1257,共11页
Flavobacterium columnare causes columnaris disease in freshwater fi sh. In the present study, the antigenic regions of fi ve outer membrane proteins(OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermo... Flavobacterium columnare causes columnaris disease in freshwater fi sh. In the present study, the antigenic regions of fi ve outer membrane proteins(OMPs), including zinc metalloprotease, prolyl oligopeptidase, thermolysin, collagenase and chondroitin AC lyase, were bioinformatically analyzed, fused together, and then expressed as a recombinant fusion protein in Escherichia coli. The expressed protein of 95.6 k Da, as estimated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was consistent with the molecular weight deduced from the amino acid sequence. The purifi ed recombinant protein was used to vaccinate the grass carp, C tenopharyngodon idella. Following vaccination of the fi sh their Ig M antibody levels were examined, as was the expression of I g M, Ig D and Ig Z immunoglobulin genes and other genes such as MHC Iα and MHC I I β, which are also involved in adaptive immunity. Interleukin genes( IL), including I L- 1β, IL- 8 and I L- 10, and type I and type II interferon(I FN) genes were also examined. At 3 and 4 weeks post-vaccination(wpv), signifi cant increases in Ig M antibody levels were observed in the fi sh vaccinated with the recombinant fusion protein, and an increase in the expression levels of I g M, Ig D and Ig Z genes was also detected following the vaccinations, thus indicating that an adaptive immune response was induced by the vaccinations. Early increases in the expression levels of IL and IFN genes were also observed in the vaccinated fi sh. At four wpv, the fi sh were challenged with F. column a re, and the vaccinated fi sh showed a good level of protection against this pathogen, with 39% relative percent survival(RPS) compared with the control group. It can be concluded, therefore, that the fi ve OMPs, in the form of a recombinant fusion protein vaccine, induced an immune response in fi sh and protection against F. columnare. 展开更多
关键词 Flavobacterium columnare outer membrane protein antigen immunogenicity vaccine immune response grass carp
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Immunogenicity and immunoprotection of recombinant PEB1 in Campylobacter-jejuni-infected mice 认领 引用 被引量：8
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作者 Lian-Feng Du Zhen-Jiang Li Xian-Ying Tang Jun-Qiong Huang Wan-Bang Sun 《World Journal of Gastroenterology》 SCIE CAS 2008年第40期6244-6248,共5页
AIM： To construct a prokaryotic expression vector carrying Campylobacterjejuni peblA gene and express it in Escherichia coli. Immunoreactivity and antigenicity of rPEB1 were evaluated. The ability of rPEB1 to induce ... AIM： To construct a prokaryotic expression vector carrying Campylobacterjejuni peblA gene and express it in Escherichia coli. Immunoreactivity and antigenicity of rPEB1 were evaluated. The ability of rPEB1 to induce antibody responses and protective efficacy was identified. METHODS： peblA gene was amplified by PCR, target gene and prokaryotic expression ptasmid pET28a （＋） was digested with BamHI and XhoI, respectively. DNA was ligated with T4 DNA ligase to construct recombinant plasmid pET28a（＋）-peblA. The rPEB1 was expressed in E. coli BL21 （DE3） and identified by SDS-PAGE. BALB/c mice were immunized with rPEBI. ELISA was used to detect the specific antibody titer and MTT method was used to measure the stimulation index of spleen lymphocyte transformation. RESULTS： The recombinant plasmid pET28a （＋）-peblA was correctly constructed. The expression output of PEB1 protein in pET28a （＋）-peblA system was approximately 33% of total proteins in E. coil The specific IgG antibody was detected in serum of BALB/c mice immunized with rPEB1 protein. Effective immunological protection with a lower sickness incidence and mortality was seen in the mice suffering from massive C. jejuni infection. CONCLUSION： rPEB1 protein is a valuable candidate for C. jejuni subunit vaccine. 展开更多
关键词 Campy/obacterjejuni Prokaryotic expression Immunogenicity
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Construction and Immunogenicity of Associated DNA Vaccine of PRRS and PCV-2 Disease 认领 引用 被引量：5
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作者 隋慧 杨金生 《Agricultural Science & Technology》 CAS 2009年第2期108-112,141,共5页
[ Objective ] The aim of the study was to construct associated DNA vaccine of PRRS （Porcine reproductive and respiratory syndrome） and PCV-2 （Porcine circovirus type 2） disease and study its immunogenicity. [ Meth... [ Objective ] The aim of the study was to construct associated DNA vaccine of PRRS （Porcine reproductive and respiratory syndrome） and PCV-2 （Porcine circovirus type 2） disease and study its immunogenicity. [ Method] In_ this study, the ORF5 gene of PRRSV isolated in Liaoning was cloned into plRES-neo expression vector, and the neo gene of plRES-neo expression vector was substituted by the ORF2 gene of the PCV-2 Mongolia strain to construct the recombinant expression vector. The expression in BHK cells was detected through Western blot and IFA. Then the ELISA antibody level and the number of spleen T lymphocytes were detected after Balb/c mice were immunized with this DNA vaccine. E Result] The recombinant plasmid plRES-ORF2-ORF5 was constructed successfully and could express the target proteins in BHK cells, as indicated by Western blot and IFA. There was no significant difference in ELISA antibody between plRES-ORF2-ORF5 immunized group and inactived vaccine immunized groups, while the number of spleen T lymphocytes induced by DNA vaccine was higher than that induced by inactived vaccine. [ Conclusion] The recombinant plasmid plRES-ORF2-ORF5 should induce good humoral immune response and cellular immune response in mice, providing the conditions for better prevention and control of PRRS and PCV-2 disease. 展开更多
关键词 PRRSV PCV-2 Associated DNA vaccine Immunogenicity
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Comparative Study on the Immunogenicity between Hsp70 DNA Vaccine and Hsp65 DNA Vaccine in Human Mycobacterium Tuberculosis 认领 引用 被引量：1
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作者 戴五星 黄海浪 +2 位作者 袁野 胡佳杰 皇甫永穆 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 2001年第3期181-183,共3页
The BALB/c mice were immunized with Hsp70 DNA and Hsp65 DNA vaccines in human Mycobacterium tuberculosis. Eight weeks after immunization, the eyeballs were removed, blood and spleen taken, and intraperitoneal macropha... The BALB/c mice were immunized with Hsp70 DNA and Hsp65 DNA vaccines in human Mycobacterium tuberculosis. Eight weeks after immunization, the eyeballs were removed, blood and spleen taken, and intraperitoneal macrophages were harvested. The lymphocytic stimulating index (SI) was used to measure the cellular proliferating ability and NO release to measure the phagocytic activity of the macrophages. With ELISA kit, the levels of interleukin-2 (IL-2) and interferon-γ (IFN-γ) in serum and the splenic lymphocytic cultured supernatant were detected. The results showed that after the mice were immunized with 100 μg/mouse of Hsp70 DNA vaccine intramuscularly, the splenic lymphocytic proliferating ability in the mice was significantly increased as compared with that in the control group, vector group and Hsp65 DNA vaccine group (P0.05); The contents of serum IFN-γ in the mice were significantly higher than in the control group, but significantly lower than in the Hsp65 DNA vaccine group (P<0.05). It was indicated that immunization with Hsp70 DNA vaccine could obviously enhance the immune response, but its intensity seemed inferior to Hsp65 DNA vaccine. The anti-infection mechanisms and clinical use in the future of the vaccines of Hsp70 DNA and Hsp65 DNA are worth further studying. 展开更多
关键词 Hsp70 DNA vaccine Hsp65 DNA vaccine immunogenicity
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