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An electrochemical immunosensor based on an antibody-ferrocene-functionalized covalent organic framework 认领 引用
1
作者 Qiang Fang Yingbo Lu +3 位作者 Jianying Huang Cheng Zhang Jing Wu Shijun Li 《Chinese Chemical Letters》 SCIE CAS CSCD 2026年第2期401-406,共6页
High-sensitive quantitative determination of alpha-fetoprotein(AFP)is of crucial importance for early clinical diagnosis of cancers.Herein,an AuNPs-free electrochemical immunosensor(Ab1-Fc-COF)was prepared from a carb... High-sensitive quantitative determination of alpha-fetoprotein(AFP)is of crucial importance for early clinical diagnosis of cancers.Herein,an AuNPs-free electrochemical immunosensor(Ab1-Fc-COF)was prepared from a carboxylic group enriched COF by post-functionalization with detecting antibody(Ab1)and ferrocene(Fc),and used for electrochemical detection of AFP.Due to the small,homogeneous pore size of the COF,Ab1 with a big size was immobilized on the surface of the COF,while Fc with a small size was covalently modified both on the surface and in the pores of COF.The covalently immobilized Ab1 was quite stable and beneficial to specifically detect AFP biomarkers.Meanwhile,the enriched Fc molecules not only improved the conductivity of the COF,but also effectively transferred and amplified the electrochemical signal.This proposed immunosensor exhibited high sensitivity in detecting AFP with a detection limit of 0.39 pg/mL(S/N of 3:1)and a wide linear response range spanning from 1 pg/mL to 100 ng/mL when plotted against logarithmic concentrations.Furthermore,this immunosensor showed excellent selectivity,stability and reproducibility in the testing of real samples.This study presents an innovative prototype for construction of a precious metal-free,antibody-directly-immobilized,simple and stable electrochemical immunoprobe. 展开更多
关键词 Covalent organic frameworks Post-functionalization Ferrocene Electrochemical immunosensors Alpha-fetoprotein
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Rapid and ultrasensitive point-of-care detection of ASFV antibodies using p30-Fc-labeled nanoparticle-based fluorescence strip-assisted portable immunosensor 认领 引用
2
作者 Yang Yang Jiayang Zheng +10 位作者 Yan Zhang Qianming Zhao Yafang Lin Junjie Zhang Zongjie Li Ke Liu Beibei Li Donghua Shao Yafeng Qiu Zhiyong Ma Jianchao Wei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2026年第6期2630-2633,共4页
Highlights A portable immunosensor device assisted by p30-Fc-labeled nanoparticle-based fiuorescence strips was developed for the rapid detection of African swine fever virus(ASFV)antibodies in the field.The fluoresce... Highlights A portable immunosensor device assisted by p30-Fc-labeled nanoparticle-based fiuorescence strips was developed for the rapid detection of African swine fever virus(ASFV)antibodies in the field.The fluorescent test strip prepared in this study has the advantages of low cost,high specificity and sensitivity,good repeatability and stability.The fiuorescent test strip detection method in this study has extremely high sensitivity and antibodies can be detected four days after ASFV infection,which has high potential application value in the monitoring and control of African swine fever. 展开更多
关键词 fiuorescence strips portable immunosensor device african swine fever ultrasensitive detection fluorescence strip rapid detection fiuorescent test strip detection p fc labeled nanoparticle
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Electrochemical immunosensor of graphene oxide/Au/Ag and strawberry-like Au@PtPd for exosome detection 认领 引用
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作者 Rong Yang Xiaorui Zhu +3 位作者 Guidan Wang Jiling Shi Xin Wang Aihua Jing 《Nanotechnology and Precision Engineering》 EI CAS CSCD 2026年第1期52-59,共8页
Early detection and treatment of colorectal cancer can effectively reduce the harm caused to patients.However,early symptoms are not obvious.Exosomes are extracellular vesicles that carry information between cells cir... Early detection and treatment of colorectal cancer can effectively reduce the harm caused to patients.However,early symptoms are not obvious.Exosomes are extracellular vesicles that carry information between cells circulating in the human body.Despite the critical importance of tumor-derived exosomes for early cancer detection,prognosis,and treatment guidance,sensitive detection of exosomes remains challenging.We report a novel immunosensor for the detection of exosomes using graphene oxide(GO)/Au/Ag as the electrochemical sensing platform and spherical Au@PtPd porous nanoparticles to provide signal amplification.A large-surface-area GO/Au/Ag-modified screen-printed carbon electrode(SPCE)is used to immobilize CD63 aptamers.The prepared strawberry-like Au@PtPd demonstrates improved sensitivity and electrocatalytic activity with increasing aptamer load.Meanwhile,its good conductivity accelerates the electron transfer rate on the SPCE.The immunosensor exhibits a linear range(100–1.0×106exosomes·μl−1)and a detection limit of 23 exosomes·μl−1.Furthermore,its potential for the detection of exosomes provides a new clinical application for early diagnosis of colorectal tumors. 展开更多
关键词 Au@PtPd Graphene/Au/Ag Exosomes Detection Immunosensor
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Signal amplificated electrochemical immunosensor for β-lactoglobulin allergen detection in dairy products applying AuNPs/Prussian blue/CMK-8 composite 认领 引用
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作者 Jingying Yang Lin Li +2 位作者 Xinmiao Ma Shuo Wang Mingfei Pan 《Food Science and Human Wellness》 SCIE CAS CSCD 2025年第8期3249-3257,共9页
Herein,a novel label-free electrochemical immunosensor was fabricated via immobilizing specific anti-β-lactoglobulin(β-LG)antibodies(Abs)onto an integrated electrode of gold nanoparticles(AuNPs)/Prussian blue(PB)/cu... Herein,a novel label-free electrochemical immunosensor was fabricated via immobilizing specific anti-β-lactoglobulin(β-LG)antibodies(Abs)onto an integrated electrode of gold nanoparticles(AuNPs)/Prussian blue(PB)/cubic Ia3d structured mesoporous carbon(CMK-8).This immunosensor allowed for the quantitative detection of the major milk allergenβ-LG.CMK-8 with excellent electrical conductivity and uniformly adjustable pore structure was modified on the glassy carbon electrode(GCE)and served as the sensitive substrate for the electro-polymerization of PB,forming the redox-active layer.AuNPs were subsequently electrochemically deposited on PB/CMK-8/GCE to improve the electrical conductivity and utilized as the connector for Abs immobilization.Duringβ-LG detection,the Abs-modified AuNPs/PB/CMK-8/GCE exhibited a significant reduction in differential pulse voltammetry current signal when exposed toβ-LG,displaying an inverse dose-dependent relationship.The developed electrochemical immunosensor demonstrated good detection performance forβ-LG,with a wider linear range of 0.01-100 ng/mL and a lower detection limit of 4.72 pg/mL.Meanwhile,the sensor exhibited remarkable repeatability,reproducibility,stability and anti-interference capabilities,which was further applied to detectβ-LG in dairy food,achieving satisfactory recoveries(89.2%-98.8%)and lower relative standard deviation(£3.1%).Therefore,this innovative electrochemical method for food allergen detection holds great potential application in food safety determination and evaluation. 展开更多
关键词 Label-free Electrochemical immunosensor Cubic Ia3d structured mesoporous carbon Prussian blue β-Lactoglobulin
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A novel impedance immunosensor based on O-phenylenediamine modified gold electrode to analyze abscisic acid 认领 引用 被引量：9
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作者 Li, Qian Wang, Ruo Zhong +2 位作者 Huang, Zhi Gang Li, He Song Xiao, Lang Tao 《Chinese Chemical Letters》 SCIE CAS 2010年第4期472-475,共4页
An impedance immunosensor based on O-phenylenediamine modified gold electrode for the determination of phytohormone abscisic acid(ABA) was proposed.The operating pH,absorption time,absorption temperature and concentra... An impedance immunosensor based on O-phenylenediamine modified gold electrode for the determination of phytohormone abscisic acid(ABA) was proposed.The operating pH,absorption time,absorption temperature and concentration of anti-ABA antibody were investigated to optimize the analytical performance.The calibration curve for the determination of ABA was obtained from this impedance immunosensor under optimal conditions.The results showed that the detection limit at about 1 ng/mL in the range of 10-5000 ng/mL... 展开更多
关键词 O-Phenylenediamine Abscisic acid Immunosensor
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A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface 认领 引用 被引量：6
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作者 Xia Sun Lu Qiao Xiangyou Wang 《Nano-Micro Letters》 SCIE EI CAS 2013年第3期191-201,共11页
A novel multilayer film based on Au nanoparticles(AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes-chitosan nanocomposite(PANI/MWCNTs/CS) was exploited to fabricate a highly sensitive immunosensor for de... A novel multilayer film based on Au nanoparticles(AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes-chitosan nanocomposite(PANI/MWCNTs/CS) was exploited to fabricate a highly sensitive immunosensor for detecting chlorpyrifos. PANI-coated MWCNTs were prepared by in situ chemical polymerization and carboxylated MWCNTs played an important role in obtaining the thin and uniform coating of PANI resulting in the improved immunosensor response. Au NPs were used as a linker to immobilize chlorpyrifos antibody. The performance of the immunosensor was characterized by means of cyclic voltammetry(CV), electrochemical impedance spectroscopy(EIS) and scanning electron microscopy(SEM), respectively. All variables involved in the preparation process and analytical performance of the immunosensor were optimized.Under optimal conditions(antibody concentration: 5 μg/mL, working buffer pH: 6.5, incubation time: 40 min,incubation temperature: 25℃), the immunosensor exhibited a wide linear range from 0.1 to 40× 10-6mg/mL and from 40 × 10-6mg/mL to 500 × 10-6mg/mL, and with a detection limit of 0.06 × 10-6mg/mL, which provided a valuable tool for the chlorpyrifos detection in real samples. 展开更多
关键词 Immunosensor AuNPs PANI/MWCNTs/CS nanocomposite Chlorpyrifos
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A MXene-functionalized paper-based electrochemical immunosensor for label-free detection of cardiac troponin I 认领 引用 被引量：5
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作者 Li Wang Yufeng Han +4 位作者 Hongchen Wang Yaojie Han Jinhua Liu Gang Lu Haidong Yu 《Journal of Semiconductors》 EI CAS CSCD 2021年第9期53-60,共8页
Convenient,rapid,and accurate detection of cardiac troponin I(cTnI)is crucial in early diagnosis of acute myocardial infarction(AMI).A paper-based electrochemical immunosensor is a promising choice in this field,becau... Convenient,rapid,and accurate detection of cardiac troponin I(cTnI)is crucial in early diagnosis of acute myocardial infarction(AMI).A paper-based electrochemical immunosensor is a promising choice in this field,because of the flexibility,porosity,and cost-efficacy of the paper.However,paper is poor in electronic conductivity and surface functionality.Herein,we report a paper-based electrochemical immunosensor for the label-free detection of cTnI with the working electrode modified by MXene(Ti3C2)nanosheets.In order to immobilize the bio-receptor(anti-cTnI)on the MXene-modified working electrode,the MXene nanosheets were functionalized by aminosilane,and the functionalized MXene was immobilized onto the surface of the working electrode through Nafion.The large surface area of the MXene nanosheets facilitates the immobilization of antibodies,and the excellent conductivity facilitates the electron transfer between the electrochemical species and the underlying electrode surface.As a result,the paper-based immunosensor could detect cTnI within a wide range of 5-100 ng/mL with a detection limit of 0.58 ng/mL.The immunosensor also shows outstanding selectivity and good repeatability.Our MXene-modified paper-based electrochemical immunosensor enables fast and sensitive detection of cTnI,which may be used in real-time and cost-efficient monitoring of AMI diseases in clinics. 展开更多
关键词 paper-based immunosensor MXene electrochemical detection cardiac troponin I(cTnI)
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Immunosensor Based on Surface Plasmon Resonance for Antigen Recognition 认领 引用 被引量：1
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作者 JIN Wei MU Ying +1 位作者 ZOU Xiang-yu JIN Qin-han 《Chemical Research in Chinese Universities》 SCIE CAS 2008年第1期24-28,共5页
A novel immunosensor based on surface plasmon resonance（SPR） has been developed for the recognition of antigen. The sensor was designed on the basis of the fixed angle of incidence and measuring the reflected intens... A novel immunosensor based on surface plasmon resonance（SPR） has been developed for the recognition of antigen. The sensor was designed on the basis of the fixed angle of incidence and measuring the reflected intensities in a wavelength range of 430--750 nm in real-time. An ultra-bright white light-emitting diode（LED） was used as the light source. Molecular self-assembling in solution was used to form the sensing membrane on gold substrate. It has been seen that the sensitivity of the SPR sensor with 3-mercaptopropionic acid（MPA）/protein A（SPA） sensing membrane is considerably higher than that with MPA or SPA modified sensing membrane. The kinetic processes on the sensing membrane were studied. The human B factor（Bf）, an activator of complement 3（C3）, was recognized among the other antigens. This sensor can also be used for other antigen/antibody or adaptoreceptor recognition. Under optimized experimental conditions, the sensor has good selectivity, repeatability, and reversibility. 展开更多
关键词 Surface plasmon resonance Immunosensor Molecular recognition
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Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer 认领 引用 被引量：1
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作者 Yongxin Liu Mengda Cao +9 位作者 Zhongxi Huang Changmin Yu Naidi Yang Qiong Wu Liang Shi Wenjie Duan Yi Zhu Jifu Wei Lin Li Wei Huang 《Chinese Chemical Letters》 SCIE CAS CSCD 2022年第4期1855-1860,共6页
Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient ser... Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient serum based on Fe3 O4@Si O2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase(HRP)-labeled antibodies linked gold nanoparticles(Au NPs) as chemiluminescence(CL) signal amplifier for ultrasensitive detection of total Ig E. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 k U/L, which is lowest in comparison with current methods, and far lower than that of Immuno CAP for Ig E detection(0.1 k U/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the Immuno CAP for the quantitative detection of Ig E, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the Ig E traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various Ig E-related diseases. 展开更多
关键词 Gold nanoparticles Magnetic nanocapturer IgE detection Ultrasensitive immunosensor Cancer
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Electrochemical Immunosensor for Detection of Atrazine Based on Polyaniline/Graphene 认领 引用 被引量：2
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作者 Nguyen Van Chu Nguyen Hai Binh +6 位作者 Cao Thi Thanh Nguyen Van Tu Nguyen Le Huy Nguyen Tuan Dzung Phan Ngoc Minh Vu Thi Thu Tran Dai Lam 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2016年第6期539-544,共6页
In this study, a novel layer-by-layer polyaniline/graphene （PANi/Gr） structure for electrochemical detec- tion of atrazine was developed. Gr film was synthesized by thermal chemical vapor deposition （CVD） method a... In this study, a novel layer-by-layer polyaniline/graphene （PANi/Gr） structure for electrochemical detec- tion of atrazine was developed. Gr film was synthesized by thermal chemical vapor deposition （CVD） method and transferred onto the PANi-predeposited microelectrode. The properties of PANi/Gr film were thoroughly investigated by high-resolution transmission electron microscopy and Raman techniques. The most attractive feature of this system is a suitable microenvironment, which could provide an amplifi- cation of the conductive signal, thus may contribute to enhancing electron transfer and subsequently improve the sensitivity in electrochemical measurements. With low detection limit （- 43 × 10 -12 g/L）, ac-ceptable stability and good reproducibility, the proposed electrochemical immunosensor could be advantageously extended for multiplexed detection of other agents of environmental pollution. 展开更多
关键词 Electrochemical immunosensor Polyaniline Graphene Atrazine
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Study on Piezoelectric Immunosensor for the Detection of H9-subtype Avian Influenza Virus 认领 引用 被引量：2
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作者 詹爱军 胡云发 +3 位作者 王新卫 刘靖清 卞红春 陈枝楠 《Agricultural Science & Technology》 CAS 2011年第10期1517-1520,共4页
[Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus（AIV）.[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid（MPA） to be m... [Objective] The aim is to develop the piezoelectric immunosensor to detect H9-subtype avian influenza virus（AIV）.[Method] The immunosensor chip was constructed by self-assembling mercaptopmpionic acid（MPA） to be monolayer on the silver-coated electrode of quartz crystal and coupling the monoclonal antibody to H9 subtype AIV with N-ethy-N＇-（3-dimethyl aminopropyl）carbodiimide hydrochloride（EDC） and N-hydroxysuccinimide（NHS）.The immunosensor to detect H9 subtype AIV was established.[Result] The results showed that the immunosensor displayed better specificity to H9 AIV and had no response to H5AIV and NDV when it was used for detection.The sensitivity test indicated the detection sensitivity for the H9 subtype AIV could reach 20-100 EID50.[Conclusion] The research provided a foundation for further research on the immunosensor for detecting AIV and it could be a new approach to detect other related viruses. 展开更多
关键词 Piezoelectric immunosensor Biological self-assembly method H9 subtype avian influenza virus
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Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor 认领 引用 被引量：2
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作者 Li-jiang WANG Chun-sheng WU Zhao-ying HU Yuan-fan ZHANG Rong LI Ping WANG 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS 2008年第2期121-131,共11页
By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance (QCM) biosensor for Escherichia coli O157:H7 detection was developed in this work. As a suitable surfactant... By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance (QCM) biosensor for Escherichia coli O157:H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid (MHDA) was introduced onto the Au surface of QCM, and then self-assembled with N-hydroxysuccinimide (NHS) raster as a reactive intermediate to provide an active interface for the specific antibody immobilization. The binding of target bacteria with the immobilized antibodies decreased the sensor’s resonant frequency, and the frequency shift was correlated to the bacterial concentration. The stepwise assembly of the immunosensor was characterized by means of the electrochemical techniques. Using the immersion-dry-immersion procedure, this QCM biosensor could detect 2.0×102 colony forming units (CFU)/ml E. coli O157:H7. In order to reduce the fabrication time, a polyelectrolyte layer-by-layer self-assembly (LBL-SA) method was adopted for fast construction. Finally, the reproducibility of this biosensor was discussed. 展开更多
关键词 Biosensor Escherichia coli O157：H7 Immunosensor Layer-by-layer self-assembly （LBL-SA） Quartz crystalmicrobalance （QCM）
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Ultrasensitive electrochemical immunosensor of carcinoembryonic antigen based on gold-label silver-stain signal amplification 认领 引用
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作者 Gaojian Yang Yuxuan Lai +2 位作者 Ziqi Xiao Congli Tang Yan Deng 《Chinese Chemical Letters》 SCIE CAS CSCD 2018年第12期1857-1860,共4页
A novel gold-label silver-stain electrochemical immunosensor was developed based on polythioninegold nanoparticles（PTh-Au） modified glassy carbon electrode（GCE） as a platform and secondary antibody labeled Au NPs... A novel gold-label silver-stain electrochemical immunosensor was developed based on polythioninegold nanoparticles（PTh-Au） modified glassy carbon electrode（GCE） as a platform and secondary antibody labeled Au NPs（Ab;-Au） as immumoprobe for carcinoembryonic antigen（CEA） detection. The sandwich-type biosensor adopted anodic stripping voltammetry to detect silver stripping signal when the Ab;-Au of the formed immunocomplexes were stained with silver. The optimized detection conditions were investigated. The effect of different electrochemical responses at various concentrations of CEA was checked by anodic stripping voltammetry. This immunosensor showed a low detection limit of 0.055 ng/mL and a wide linear calibration of 0.1-120 ng/mL（R;=0.99856）. Moreover, this immunoassay also existed the advantages of good reproducibility, stability and selectivity. Thus, this immunosensing protocol may provide a potential application for effective clinical detection of CEA. 展开更多
关键词 Gold-label silver-stain Carcinoembryonic antigen Sandwich-type immunoassay Electrochemical immunosensor Anodic stripping voltammetry
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A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle 认领 引用
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作者 Ying Zhang Cong Li +5 位作者 Yi Liu Lin Chu Juncheng Zou Youqi Yan Xin Hu Xiaomei Ling 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2021年第3期239-245,共7页
Human apolipoprotein E4(APOE4)is an important risk factor for late-onset Alzheimer’s disease(AD).However,little progress has been made for the detection of APOE4,and most of existing detection methods suffer from tim... Human apolipoprotein E4(APOE4)is an important risk factor for late-onset Alzheimer’s disease(AD).However,little progress has been made for the detection of APOE4,and most of existing detection methods suffer from time-consuming process and expensive instruments.This study firstly proposed a simple and sensitive electrochemical method for detection of APOE4 based on carboxyl-rich CeZnO nanoparticles.Under the optimal conditions,the fabricated immunosensor exhibited a good linear relationship ranging from 10 to 100 ng/mL with the detection limit of 1.8 ng/mL(S/N=3).The proposed electrochemical immunosensor had excellent selectivity,reproducibility and stability.Good performance was observed for sensitive determination of APOE4 in human serum sample,which provided a strong support for the detection of APOE4 and early clinical prevention of AD. 展开更多
关键词 APOE4 CeZnO nanoparticle Alzheimer’s disease Label-free immunosensor
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A competitive microcystin-LR immunosensor based on Au NPs@metal-organic framework(MIL-101) 认领 引用 被引量：2
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作者 Kunlei Zhang Kun Dai +6 位作者 Ruyan Bai Yuchan Ma Yan Deng Delei Li Xi Zhang Rong Hu Yunhui Yang 《Chinese Chemical Letters》 SCIE CAS CSCD 2019年第3期664-667,共4页
An electrochemical immunosensor was developed for ultrasensitive detection of microcystin-LR in water. MIL-101, a porous metal-organic frameworks(MOFs) material based on trivalent chromium skeleton were synthesized by... An electrochemical immunosensor was developed for ultrasensitive detection of microcystin-LR in water. MIL-101, a porous metal-organic frameworks(MOFs) material based on trivalent chromium skeleton were synthesized by hydrothermal synthesis method, and loaded with Au nanoparticles(Au NPs) to prepare Au NPs@MIL-101 composite materials which were used as a marker to label anti microcystin-LR(Anti-MC-LR). The composite materials have strong catalytic properties to the oxidation of ascorbic acid. Anti-MC-LR was immobilized on glassy carbon electrode surface using electrodeposition graphene oxide(GO) as an immobilization matrix to construct a competitive microcystin-LR immunosensor. The electrochemical immunosensor display linear relationship in the range of 0.05 ng/mL-75 μg/mL with linear correlation coefficient of 0.9951 and detection limit of 0.02 ng/mL(S/N = 3). This sensor was used to detect microcystin-LR in the water sample. The recovery was 102.43%,which is satisfied. The good testing results indicate the sensor has a great prospect in practical application. 展开更多
关键词 Microcystin-LR Metal-organic frameworks MIL-101 Competitive Electrochemical immunosensor
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A novel α-fetoprotein-MIP immunosensor based on AuNPs/PTh modified glass carbon electrode 认领 引用 被引量：2
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作者 Yuxuan Lai Chuanxiang Zhang +4 位作者 Yan Deng Gaojian Yang Song Li Congli Tang Nongyue He 《Chinese Chemical Letters》 SCIE CAS CSCD 2019年第1期160-162,共3页
A new α-fetoprotein-MIP(AFP-MIP) immunosensor based on glass carbon electrode(GCE) modified with polythionine(PTh) and gold nanoparticles(AuNPs) was successfully fabricated for sensitive detection ofα-fetoprotein(AF... A new α-fetoprotein-MIP(AFP-MIP) immunosensor based on glass carbon electrode(GCE) modified with polythionine(PTh) and gold nanoparticles(AuNPs) was successfully fabricated for sensitive detection ofα-fetoprotein(AFP). Through controlling electropolymerization, A "polydopamine(PDA)-AFP" complex was achieved applying AFP as template and dopamine(DA) as imprinted monomers. After elution, the specific cavities can adsorb the target molecules. Using differential pulse voltammetry(DPV) detection,the peak current decreased with the increase in concentration of AFP, and the linear response range of the AFP-MIP immunosensor was from 0.001 ng/mL to 800 ng/mL with the detection limit as low as0.8138 pg/mL. The MIP immunosensor could become a new promising method for the detection of AFP.Furthermore, this MIP sensor was demonstrated in testing AFP in human serum samples with satisfactory results. 展开更多
关键词 Molecular imprinting polymer α-Fetoprotein Electropolymerization Differential pulse voltammetry Immunosensor
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Diagnostic tests for hepatitis C: Recent trends in electrochemical immunosensor and genosensor analysis 认领 引用 被引量：3
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作者 Carolina V Uliana Carla S Riccardi Hideko Yamanaka 《World Journal of Gastroenterology》 SCIE CAS 2014年第42期15476-15491,共16页
Hepatitis C is a liver disease that is transmitted through contact with the blood of an infected person. An estimated 150 million individuals worldwide have been chronically infected with the hepatitis C virus(HCV). H... Hepatitis C is a liver disease that is transmitted through contact with the blood of an infected person. An estimated 150 million individuals worldwide have been chronically infected with the hepatitis C virus(HCV). Hepatitis C shows significant genetic variation in the global population, due to the high rate of viral RNA mutation. There are six variants of the virus(HCV genotypes 1, 2, 3, 4, 5, and 6), with 15 recorded subtypes that vary in prevalence across different regions of the world. A variety of devices are used to diagnose hepatitis C, including HCV antibody test, HCV viral load test, HCV genotype test and liver biopsy. Rapid, inexpensive, sensitive, and robust analytical devices are therefore essential for effective diagnosis and monitoring of disease treatment. This review provides an overview of current electrochemical immunosensor and genosensortechnologies employed in HCV detection.There are a limited number of publications showing electrochemical biosensors being used for the detection of HCV.Due to their simplicity,specificity,and reliability,electrochemical biosensor devices have potential clinical applications in several viral infections. 展开更多
关键词 Hepatitis C virus Immunosensors Genosensors Electrochemical detection Diagnostic tests
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Novel photoelectrochemical immunosensor for MCF-7 cell detection based on n-p organic semiconductor heterojunction 认领 引用 被引量：1
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作者 Qin Zeng Qingya Wei +4 位作者 Jiarong Luo Yong Qian Minghui Yang Yingping Zou Limin Lu 《Chinese Chemical Letters》 SCIE CAS CSCD 2022年第6期2954-2958,共5页
In this work,we developed a novel photoelectrochemical(PEC)sensor based on n-p organic semiconductor heterojunction for sensitive detecting MCF-7 cancer cells.BTA-C4 Ph and PM6 were designed as photoactive materials t... In this work,we developed a novel photoelectrochemical(PEC)sensor based on n-p organic semiconductor heterojunction for sensitive detecting MCF-7 cancer cells.BTA-C4 Ph and PM6 were designed as photoactive materials to form n-p heterojunction,which greatly enhanced the photoelectric conversion efficiency.Antibody-modified magnetic nanoparticles were utilized to capture and separate MCF-7 cells from samples.Detection of MCF-7 is ascribed to the loading of MCF-7 onto BTA-C4 Ph-PM6 modified electrode that resulted in the decrease of photocurrent intensity.The PEC immunosensor displayed a linear concentration ranging from 50 cell/m L to 1×104 cell/m L with a limit of detection(LOD)of 41 cell/m L(S/N=3)for MCF-7.Additionally,the senor also exhibited good stability,excellent selectivity and prominent reproducibility.Furthermore,the sensor was successfully applied to detect MCF-7 in whole blood.This work illustrates that n-p heterojunction of organic semiconductor may find wide applications for the preparation of different photoelectrochemical sensors. 展开更多
关键词 Photoelectrochemical immunosensor Organic semiconductors n-p Heterojunction MCF-7
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printed electrode immunosensors for Schistosoma japonicum 认领 引用 被引量：7
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作者 ZENG Shaohua TIAN Zhi +7 位作者 CHE Hongli YANG Huihui CHEN Xiuchun FENG Qimei ZHOU Yunfei ZHANG Shuju WU Zhaoyang WANG Shiping 《中南大学学报（医学版）》 CAS CSCD 北大核心 2012年第6期541-548,共8页
Objective： To improve the sensitivity and the linear range of electrochemical immunosensor to detect Schistosoma ]aponicum （S. ]aponicum） antibody.Methods： Carbon inks and silver/silver chloride inks were printed ... Objective： To improve the sensitivity and the linear range of electrochemical immunosensor to detect Schistosoma ]aponicum （S. ]aponicum） antibody.Methods： Carbon inks and silver/silver chloride inks were printed on a polyethylene terephthalate （PET） board to make a two-electrode test strip, where carbon was the working electrode and S. japonicum soluble egg antigen （SEA） was fixed at one end of working electrode by different methods; silver/silver chloride electrode was used as control We tested the valency of the antibody by cyclic voltammetry （CV） and differential pulse voltammetry （DPV） in an electrochemistry workstation, and conducted comparison with the results of ELISA. Two new immunosensing electrodes have been developed, based on glutaraldehyde cross-linked （GA） or chitosan-glutaraldehyde cross-linked （Chit-GA） transducer fixing S. japonicum antigen. We tested the titer of the antibody by means of CV and DPV.Results： Our experimental S. japonicum antigen （50 μg/L） is the optimal test concentration for the GA sensor, and 10 μg/L for Chit-GA sensors. The immune reaction time of both electrodes is all essentially complete in 1 minute. The linear range for S.japonicum antibody in human positive serum sample detection by the glutaraldehyde cross-linked immunosensor is 1：1000 to 1：400, and by the chitosan-glutaraldehyde cross-linked immunosensor is 1：1000 to 1：500. As the concentration of dilution ratio of S. japonicum antibody in human positive serum sample increased, the test value of DPV increased proportionally.Conclusion： GA sensor and Chit-GA cross-linked S. jaloonicum sensors have high sensitivity and broad linear range response, and both exhibited a good linear relationship between the DPV signal and the test antibody titer. 展开更多
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Monitoring Domoic Acid in Marine Phytoplankton by Disposable Immunosensors 认领 引用
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作者 Roberta Congestri Laura Micheli Giuseppe Palleschi 《American Journal of Plant Sciences》 CAS 2017年第5期1077-1091,共15页
Advances in sensor technology have allowed the significant progress in the monitoring of noxious compounds in the sea, providing real-time detection so as to prevent risks associated with the diffusion and dispersion ... Advances in sensor technology have allowed the significant progress in the monitoring of noxious compounds in the sea, providing real-time detection so as to prevent risks associated with the diffusion and dispersion of toxic substances in the environment. An important element in the overall picture is the harmful algal blooms which pose serious threats to marine ecosystems through the production of toxins that accumulate in filter-feeders and ultimately impact both human health and fisheries. Domoic acid is a neurotoxic amino acid produced by marine planktonic diatoms of the genus Pseudo-nitzschia. Here we monitored domoic acid production by natural Pseudo-nitzschia populations in phytoplankton samples collected along the Middle Tyrrhenian coast, over the course of one year, using selective immunosensors based on screenprinted electrodes, using differential pulse voltammetry as the electrochemical technique, to yield quantitative outputs. In this work, disposable devices have been applied for monitoring the production of domoic acid on algal extracts and the results have been validated by conventional high pressure liquid chromatography-ultraviolet detection methods. The data obtained revealed the presence of domoic acid in Italian phytoplankton, especially in coastal impacted areas, highlighting the potential risk of toxin entering into marine food webs and the environment. Immunosensors based on screen-printed electrodes prove to be effective tools for annual monitoring of domoic acid in seawater samples, thus providing a reliable early warning system relative to health and economic impact of algal toxins. 展开更多
关键词 Domoic Acid Monitoring Immunosensors Screen Printed Electrodes Toxic Pseudo-nitzschia
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