Enhancing net ecosystem productivity(NEP)and water yield(WY)services is critical for sustainable ecosystem management and water security.In 2010,China established National Key Ecological Function Zones(NKEFZs)to resto...Enhancing net ecosystem productivity(NEP)and water yield(WY)services is critical for sustainable ecosystem management and water security.In 2010,China established National Key Ecological Function Zones(NKEFZs)to restore ecosystems.However,their impacts on carbon-water services dynamics remains poorly quantified.Using a calibrated process-based model(CEVSA-ES),we assessed the effects of vegetation restoration(greening and vegetation type changes)and global climate changes(climate change,elevated CO2,and nitrogen deposition)on the shifts in NEP and WY trends relative to NKEFZ implementation.Over 2001-2021,both NEP and WY exhibited increasing trends(5.1 Tg C yr-2and 0.3 mm yr-1,respectively),and were the most evident in the water and soil conservation zones,biodiversitymaintenance zones,and water conservation zones,respectively.Notably,following the NKEFZs establishment,NEP growth accelerated remarkably from 1.9 Tg C yr-2(2001-2010)to 5.6 Tg C yr-2(2011-2021),particularly within water conservation zones,whereas WY trends reversed from a decline(-0.5 mm yr-1)to an increase(0.9 mm yr-1).While greening drove NEP growth and precipitation governed WY changes during 2001-2021,the post-2010 NEP acceleration was jointly controlled by vegetation restoration and global climate change.Conversely,theWY trend reversal was primarily attributed to shifts in precipitation trends.These findings provide critical insights into how ecological policies can synergistically enhance carbon and water services under a changing climate,offering important implications for sustainable ecological restoration and natural climate solutions.展开更多
The balance between ecological conservation and economic development holds immense significance in crucial ecological function zones.The framework of Coupled Human and Natural Systems(CHANS)offers a valuable tool to e...The balance between ecological conservation and economic development holds immense significance in crucial ecological function zones.The framework of Coupled Human and Natural Systems(CHANS)offers a valuable tool to evaluate the synchronization between the natural subsystem and the human subsystem.This research presents a case study that analyzes the ecological conservation effectiveness and strategies of Nanling key ecological function zone of China from the perspective ofCHANS.The findings reveal that over the last two decades,the stability of ecosystem services in Nanling has coexisted with a significant increase in human activities.The correlation coefficient between ecosystem services index(ESI)and human activity index(HAI)has decreased from-0.6034 to-0.6139,indicating a continuously strengthening negative correlation.The spatial differentiation between the nature subsystemand the human subsystemhas also intensified.The coupling coordination degree(D)has increased from0.3259 to 0.3667,representing a 12.50%improvement.This suggests that the overall coupling coordination of CHANS in Nanling has been enhancing.The coupling relationships between ESI and HAI were categorized into four groups.Among these,21.3%of the areas fall into the positive trade-off quadrant,while 13.0%fall into the negative trade-off quadrant.Overall,climate factors promote positive trade-offs,while human activities promote negative trade-offs.Therefore,it is crucial to strengthen anthropogenic vegetation restoration and reduce construction land expansion as important measures to promote ecological conservation in Nanling key ecological function zone.As a conclusion,despite a significant increase in human activity intensity,ecosystem services remain stable.The ecological conservation effectiveness in Nanling key ecological function zone has achieved remarkable results.展开更多
Iris japonica,an evergreen ornamental species known for its beautiful flowers and year-round evergreen foliage,is difficult to genetically manipulate due to the lack of an efficient genetic transformation system.This ...Iris japonica,an evergreen ornamental species known for its beautiful flowers and year-round evergreen foliage,is difficult to genetically manipulate due to the lack of an efficient genetic transformation system.This limitation hinders the functional verification of key genes in I.japonica.To address this,a Virus-Induced Gene Silencing(VIGS)system based on Tobacco rattle virus(TRV)was developed,enabling efficient,transient gene silencing in I.japonica.In this system,the phytoene desaturase(PDS)gene,a common reporter gene used for VIGS,was selected to assess the silencing efficiency.The recombinant vector pTRV2-IjPDS was used to infect I.japonica plants.The PDS gene silencing resulted in distinct photobleaching symptoms,demonstrating the successful silencing of the target gene.The presence of the TRV vector was verified by using a GFP-tagged pTRV2-GFP vector,and GFP expression was detected using fluorescence visualization under ultraviolet(UV)light and confirmed by laser confocal microscopy.Real-time PCR was used to quantify the reduction in IjPDS gene expression,confirming the successful silencing.The study further optimized the VIGS system by evaluating the impact of seedling ages on silencing efficiency,identifying one-year-old seedlings as the most effective for gene silencing(36.67%).This TRV-based VIGS system provides a robust tool for functional gene analysis in I.japonica and offers a new approach to studying the rules of key genes in its biological processes,with potential applications in ornamental plant breeding and genomics research.展开更多
This article deals with the reflective function of the differential systems. The results are applied to discussion of the existence and stability of the periodic solutions of these systems.
With the reduction of sequencing costs,optimization of algorithms,and improvement of multi-omics integration capabilities,transcriptomics,as a core technology for analyzing gene expression dynamics and discovering key...With the reduction of sequencing costs,optimization of algorithms,and improvement of multi-omics integration capabilities,transcriptomics,as a core technology for analyzing gene expression dynamics and discovering key functional molecules,has shown great potential in the field of disease prevention and control[1,2].The multi-continental transcriptomics study of tick-borne poxvirus not only provides a new perspective for understanding the evolution and transmission of vector-mediated viruses,but also reflects the trend of transcriptomics research and highlights its key role in disease prevention and control[3].展开更多
From the club-like limbs of the mantis shrimp to the texture of a cicada's wing,recently reported studies of the structures found in living creatures are fostering innovations in synthetic materials.The work shows...From the club-like limbs of the mantis shrimp to the texture of a cicada's wing,recently reported studies of the structures found in living creatures are fostering innovations in synthetic materials.The work shows how biological research can underpin the development of novel materials that adopt key attributes or functions of natural substances with the goal of applying them in improved,often high-technology products[1].展开更多
Soil erosion is a significant factor in the deterioration of the ecological environment and soil conservation is an important ecological service of National Key Ecological Function Areas in China. Here, climate, terra...Soil erosion is a significant factor in the deterioration of the ecological environment and soil conservation is an important ecological service of National Key Ecological Function Areas in China. Here, climate, terrain, soil and vegetation cover, soil erosion and soil conservation spatial data in 25 National Key Ecological Function Areas in 2010 were analyzed using the Universal Soil Loss Equation by Arc GIS tool. We found that soil conservation effects due to vegetation cover and soil conservation measures are obvious and that micro and slight erosion areas in National Key Ecological Function Areas have increased by 26.2%. The area of intensive erosion decreased by 25.1%, and the soil conservation amount in southern National Key Ecological Function Areas is high. The conservation amount of soil nutrients within National Key Ecological Function Areas is related to amount of soil conservation and content of each nutrient element in soil. The sequence of nutrient conservation amounts from high to low is soil organic carbon, total K, total N and total P in soil. The conservation amount of various soil nutrient elements in the Alkin Grassland function area and Yunnan and Sichuan function area was highest. Based on our findings, we recommend the strengthening of vegetation protection and management in the areas with high per unit area soil erosion, enhancement of vegetation cover and improvement of soil conservation measures in order to improve soil conservation functions and reduce soil nutrient losses. Vegetation protection and soil conservation measures should be consolidated in the areas with high potential erosion to prevent further deterioration.展开更多
Ecosystem services are transferred from the service-providing area to the service-benefiting area to satisfy human needs through some substance, energy or information. Most studies focus on the provision of ecosystem ...Ecosystem services are transferred from the service-providing area to the service-benefiting area to satisfy human needs through some substance, energy or information. Most studies focus on the provision of ecosystem services and few focus on the demands on ecosystem services and their spatial distribution. Here, on the basis of the flow of water conservation services from the providing area to the benefiting area, the benefits produced by water conservation service are investigated and the benefiting areas are identified. The results indicate that in 2010 the water conservation service of key ecological function areas provided irrigation water for 1.67×105 km2 of paddy fields and 1.01×105 km2 irrigated fields, domestic water to urban residents and industrial water to factories, mines and enterprises of 2.64×104 km2 urban construction land and domestic water to rural residents across 3.73×104 km2 of rural settlements and formed 6.64×104 km2 of inland water which can be used for freshwater aquaculture, downstream regions comprise 1.31×104 km of navigable river, which can be used for inland shipping. The benefit areas of the key function areas located in the upper and middle reaches of the Yangtze River are greater and more influential benefit areas. To protect these key function areas, more attention should be paid to the maintenance and improvement of water conservation. Some benefit areas have access to the benefits produced by water conservation of nine key ecological function areas and cover 17% of the overall benefit area and the length of their channels benefited accounts for 7%. Multiple key ecological function areas should be taken into account equally in the formulation of ecological compensation policies. These research findings can serve as a scientific basis for the compensated use of and ecological compensation for ecosystem services provided by key ecological function areas.展开更多
At the end of the 1990 s China started to construct an ecological compensation system for national key ecological function areas. An eco-compensation system focusing on the "Transfer Payment of National Key Ecologica...At the end of the 1990 s China started to construct an ecological compensation system for national key ecological function areas. An eco-compensation system focusing on the "Transfer Payment of National Key Ecological Function Areas" has been established. The current eco-compensation system has played a positive role in improving the ecological environment of national key ecological function areas; but the ecological effect is weakening due to existing problems in the eco-compensation system such as unreasonable calculation of compensation standards, poor expression of rights and interests of stakeholders, simple sources of capital and modes of compensation. For better development, the eco-compensation system should be improved by(i) adhering to the combination of vertical compensation(oriented) and horizontal compensation(auxiliary) on the whole and establishing a transfer payment system of eco-compensation at national scale;(ii) defining the compensation standard according to ecological protection costs, development opportunity costs and ecological service values and adding indicators that reflect economic green transformation to evaluate compensation effects;(iii) gradually building a utilization system of eco-compensation funds in which multi-stakeholders participate and establishing a green development-oriented system to evaluate the performance of local governments; and(iv) cultivating a new business pattern where ecological services are the product, to grow the ecological service industry.展开更多
In order to establish the sufficient and necessary condition that arbitrarily reliable systems can not be constructed with function elements under interference sources, it is very important to expand set of interferen...In order to establish the sufficient and necessary condition that arbitrarily reliable systems can not be constructed with function elements under interference sources, it is very important to expand set of interference sources with the above property. In this paper, the models of two types of interference sources are raised respectively: interference source possessing real input vectors and constant reliable interference source. We study the reliability of the systems effected by the interference sources, and the lower bound of the reliability is presented. The results show that it is impossible that arbitrarily reliable systems can not be constructed with the elements effected by above interference sources.展开更多
Raw-materials based broad bean paste(rmBBP)is popular among consumers.Though high salinity can select functional microbial community for fermentation,it also impaired the fermentation efficiency of rmBBP and unfavored...Raw-materials based broad bean paste(rmBBP)is popular among consumers.Though high salinity can select functional microbial community for fermentation,it also impaired the fermentation efficiency of rmBBP and unfavored human health.Therefore,this study aimed to construct a synthetic microbial community based on key functional microbes for reduced salt rmBBP fermentation based on the elucidation of the microbial community succession during fermentation.Based on the tracked determination and multivariate statistical analysis of the physicochemical parameters,metabolites and the structure of microbial community,salinity and microbial in-teractions were key drivers for the succession of microbial community.Aspergillus,Staphylococcus,Weissella and Tetragenococcus were predicted to play key roles during fermentation.After that,core species were isolated from rmBBP mash and their salinity tolerance and metabolic characteristics were evaluated.Finally,a synthetic mi-crobial community(Aspergillus oryzae,Staphylococcus carnosus,Tetragenococcus halophilus and Weissella confusa)was constructed and applied in reduced-salt rmBBP fermentation.The bioaugmentation contributed to the accumulation of volatile metabolites while effectively maintaining the acidity during rmBBP fermentation when salinity was reduced from 12%to 10%.In general,this study developed a synthetic microbial community with desired characteristics for successful reduced-salinity rmBBP fermentation.展开更多
基金funded by the and National and Natural Science Foun-dation of China(Grants No.42501597,42141005 and 42030509)the China Postdoctoral Science Foundation(Grants No.2025M770371 and 2025T180098).
摘要Enhancing net ecosystem productivity(NEP)and water yield(WY)services is critical for sustainable ecosystem management and water security.In 2010,China established National Key Ecological Function Zones(NKEFZs)to restore ecosystems.However,their impacts on carbon-water services dynamics remains poorly quantified.Using a calibrated process-based model(CEVSA-ES),we assessed the effects of vegetation restoration(greening and vegetation type changes)and global climate changes(climate change,elevated CO2,and nitrogen deposition)on the shifts in NEP and WY trends relative to NKEFZ implementation.Over 2001-2021,both NEP and WY exhibited increasing trends(5.1 Tg C yr-2and 0.3 mm yr-1,respectively),and were the most evident in the water and soil conservation zones,biodiversitymaintenance zones,and water conservation zones,respectively.Notably,following the NKEFZs establishment,NEP growth accelerated remarkably from 1.9 Tg C yr-2(2001-2010)to 5.6 Tg C yr-2(2011-2021),particularly within water conservation zones,whereas WY trends reversed from a decline(-0.5 mm yr-1)to an increase(0.9 mm yr-1).While greening drove NEP growth and precipitation governed WY changes during 2001-2021,the post-2010 NEP acceleration was jointly controlled by vegetation restoration and global climate change.Conversely,theWY trend reversal was primarily attributed to shifts in precipitation trends.These findings provide critical insights into how ecological policies can synergistically enhance carbon and water services under a changing climate,offering important implications for sustainable ecological restoration and natural climate solutions.
基金supported by the National Natural Science Foundation of China(No.42201323)Science and Technology Program of Guangzhou,China(No.2023A04J0252)the National Key R&D Program of China(2022YFF1301204).
摘要The balance between ecological conservation and economic development holds immense significance in crucial ecological function zones.The framework of Coupled Human and Natural Systems(CHANS)offers a valuable tool to evaluate the synchronization between the natural subsystem and the human subsystem.This research presents a case study that analyzes the ecological conservation effectiveness and strategies of Nanling key ecological function zone of China from the perspective ofCHANS.The findings reveal that over the last two decades,the stability of ecosystem services in Nanling has coexisted with a significant increase in human activities.The correlation coefficient between ecosystem services index(ESI)and human activity index(HAI)has decreased from-0.6034 to-0.6139,indicating a continuously strengthening negative correlation.The spatial differentiation between the nature subsystemand the human subsystemhas also intensified.The coupling coordination degree(D)has increased from0.3259 to 0.3667,representing a 12.50%improvement.This suggests that the overall coupling coordination of CHANS in Nanling has been enhancing.The coupling relationships between ESI and HAI were categorized into four groups.Among these,21.3%of the areas fall into the positive trade-off quadrant,while 13.0%fall into the negative trade-off quadrant.Overall,climate factors promote positive trade-offs,while human activities promote negative trade-offs.Therefore,it is crucial to strengthen anthropogenic vegetation restoration and reduce construction land expansion as important measures to promote ecological conservation in Nanling key ecological function zone.As a conclusion,despite a significant increase in human activity intensity,ecosystem services remain stable.The ecological conservation effectiveness in Nanling key ecological function zone has achieved remarkable results.
基金supported by the general project of the Zhejiang Provincial Department of Education(Grant No.Y202456763)the Zhejiang Sci-Tech University Start-up Fund(Grant No.24052162-Y)+1 种基金the China Postdoctoral Science Foundation(Grant No.2021M692790)the National Natural Science Foundation of China(NSFC)(Grant No.31901352).
摘要Iris japonica,an evergreen ornamental species known for its beautiful flowers and year-round evergreen foliage,is difficult to genetically manipulate due to the lack of an efficient genetic transformation system.This limitation hinders the functional verification of key genes in I.japonica.To address this,a Virus-Induced Gene Silencing(VIGS)system based on Tobacco rattle virus(TRV)was developed,enabling efficient,transient gene silencing in I.japonica.In this system,the phytoene desaturase(PDS)gene,a common reporter gene used for VIGS,was selected to assess the silencing efficiency.The recombinant vector pTRV2-IjPDS was used to infect I.japonica plants.The PDS gene silencing resulted in distinct photobleaching symptoms,demonstrating the successful silencing of the target gene.The presence of the TRV vector was verified by using a GFP-tagged pTRV2-GFP vector,and GFP expression was detected using fluorescence visualization under ultraviolet(UV)light and confirmed by laser confocal microscopy.Real-time PCR was used to quantify the reduction in IjPDS gene expression,confirming the successful silencing.The study further optimized the VIGS system by evaluating the impact of seedling ages on silencing efficiency,identifying one-year-old seedlings as the most effective for gene silencing(36.67%).This TRV-based VIGS system provides a robust tool for functional gene analysis in I.japonica and offers a new approach to studying the rules of key genes in its biological processes,with potential applications in ornamental plant breeding and genomics research.
摘要This article deals with the reflective function of the differential systems. The results are applied to discussion of the existence and stability of the periodic solutions of these systems.
摘要With the reduction of sequencing costs,optimization of algorithms,and improvement of multi-omics integration capabilities,transcriptomics,as a core technology for analyzing gene expression dynamics and discovering key functional molecules,has shown great potential in the field of disease prevention and control[1,2].The multi-continental transcriptomics study of tick-borne poxvirus not only provides a new perspective for understanding the evolution and transmission of vector-mediated viruses,but also reflects the trend of transcriptomics research and highlights its key role in disease prevention and control[3].
摘要From the club-like limbs of the mantis shrimp to the texture of a cicada's wing,recently reported studies of the structures found in living creatures are fostering innovations in synthetic materials.The work shows how biological research can underpin the development of novel materials that adopt key attributes or functions of natural substances with the goal of applying them in improved,often high-technology products[1].
基金the National Science and Technology Support Program(2013BAC03B05)the National Natural Science Foundation of China(41101539)
摘要Soil erosion is a significant factor in the deterioration of the ecological environment and soil conservation is an important ecological service of National Key Ecological Function Areas in China. Here, climate, terrain, soil and vegetation cover, soil erosion and soil conservation spatial data in 25 National Key Ecological Function Areas in 2010 were analyzed using the Universal Soil Loss Equation by Arc GIS tool. We found that soil conservation effects due to vegetation cover and soil conservation measures are obvious and that micro and slight erosion areas in National Key Ecological Function Areas have increased by 26.2%. The area of intensive erosion decreased by 25.1%, and the soil conservation amount in southern National Key Ecological Function Areas is high. The conservation amount of soil nutrients within National Key Ecological Function Areas is related to amount of soil conservation and content of each nutrient element in soil. The sequence of nutrient conservation amounts from high to low is soil organic carbon, total K, total N and total P in soil. The conservation amount of various soil nutrient elements in the Alkin Grassland function area and Yunnan and Sichuan function area was highest. Based on our findings, we recommend the strengthening of vegetation protection and management in the areas with high per unit area soil erosion, enhancement of vegetation cover and improvement of soil conservation measures in order to improve soil conservation functions and reduce soil nutrient losses. Vegetation protection and soil conservation measures should be consolidated in the areas with high potential erosion to prevent further deterioration.
基金the National Science and Technology Support Program(2013BAC03B05)the National Natural Science Foundation of China(31400411)
摘要Ecosystem services are transferred from the service-providing area to the service-benefiting area to satisfy human needs through some substance, energy or information. Most studies focus on the provision of ecosystem services and few focus on the demands on ecosystem services and their spatial distribution. Here, on the basis of the flow of water conservation services from the providing area to the benefiting area, the benefits produced by water conservation service are investigated and the benefiting areas are identified. The results indicate that in 2010 the water conservation service of key ecological function areas provided irrigation water for 1.67×105 km2 of paddy fields and 1.01×105 km2 irrigated fields, domestic water to urban residents and industrial water to factories, mines and enterprises of 2.64×104 km2 urban construction land and domestic water to rural residents across 3.73×104 km2 of rural settlements and formed 6.64×104 km2 of inland water which can be used for freshwater aquaculture, downstream regions comprise 1.31×104 km of navigable river, which can be used for inland shipping. The benefit areas of the key function areas located in the upper and middle reaches of the Yangtze River are greater and more influential benefit areas. To protect these key function areas, more attention should be paid to the maintenance and improvement of water conservation. Some benefit areas have access to the benefits produced by water conservation of nine key ecological function areas and cover 17% of the overall benefit area and the length of their channels benefited accounts for 7%. Multiple key ecological function areas should be taken into account equally in the formulation of ecological compensation policies. These research findings can serve as a scientific basis for the compensated use of and ecological compensation for ecosystem services provided by key ecological function areas.
摘要At the end of the 1990 s China started to construct an ecological compensation system for national key ecological function areas. An eco-compensation system focusing on the "Transfer Payment of National Key Ecological Function Areas" has been established. The current eco-compensation system has played a positive role in improving the ecological environment of national key ecological function areas; but the ecological effect is weakening due to existing problems in the eco-compensation system such as unreasonable calculation of compensation standards, poor expression of rights and interests of stakeholders, simple sources of capital and modes of compensation. For better development, the eco-compensation system should be improved by(i) adhering to the combination of vertical compensation(oriented) and horizontal compensation(auxiliary) on the whole and establishing a transfer payment system of eco-compensation at national scale;(ii) defining the compensation standard according to ecological protection costs, development opportunity costs and ecological service values and adding indicators that reflect economic green transformation to evaluate compensation effects;(iii) gradually building a utilization system of eco-compensation funds in which multi-stakeholders participate and establishing a green development-oriented system to evaluate the performance of local governments; and(iv) cultivating a new business pattern where ecological services are the product, to grow the ecological service industry.
基金Tsinghua University Research Foundation(JC2000025)
摘要In order to establish the sufficient and necessary condition that arbitrarily reliable systems can not be constructed with function elements under interference sources, it is very important to expand set of interference sources with the above property. In this paper, the models of two types of interference sources are raised respectively: interference source possessing real input vectors and constant reliable interference source. We study the reliability of the systems effected by the interference sources, and the lower bound of the reliability is presented. The results show that it is impossible that arbitrarily reliable systems can not be constructed with the elements effected by above interference sources.
基金supported by the National Natural Science Foundation of China(No.32272282)Yunnan Province Science and Technology department(No.202305AF150007)Key Laboratory of Industrial Biotechnology of Ministry of Education in Jiangnan Univer-sity(No.KLIB-KF202208).
摘要Raw-materials based broad bean paste(rmBBP)is popular among consumers.Though high salinity can select functional microbial community for fermentation,it also impaired the fermentation efficiency of rmBBP and unfavored human health.Therefore,this study aimed to construct a synthetic microbial community based on key functional microbes for reduced salt rmBBP fermentation based on the elucidation of the microbial community succession during fermentation.Based on the tracked determination and multivariate statistical analysis of the physicochemical parameters,metabolites and the structure of microbial community,salinity and microbial in-teractions were key drivers for the succession of microbial community.Aspergillus,Staphylococcus,Weissella and Tetragenococcus were predicted to play key roles during fermentation.After that,core species were isolated from rmBBP mash and their salinity tolerance and metabolic characteristics were evaluated.Finally,a synthetic mi-crobial community(Aspergillus oryzae,Staphylococcus carnosus,Tetragenococcus halophilus and Weissella confusa)was constructed and applied in reduced-salt rmBBP fermentation.The bioaugmentation contributed to the accumulation of volatile metabolites while effectively maintaining the acidity during rmBBP fermentation when salinity was reduced from 12%to 10%.In general,this study developed a synthetic microbial community with desired characteristics for successful reduced-salinity rmBBP fermentation.