期刊文献+
任意字段
题名或关键词
题名
关键词
摘要
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息
共找到773篇文章
< 1 2 … 39 >
每页显示 20 50 100
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
显示方式：
Identification of SRSF2 as a potential immunodiagnostic biomarker for osteosarcoma:A study based on focused protein microarray 认领 引用
1
作者 Yan Ma Lei Wan +7 位作者 Yong-Yong Zhang Ya-Ge Luo Ning Yang Fei-Fei Pu Malik Ihsan Ullah Khan Man Liu Jin-Shan Liu Ji-Tian Li 《World Journal of Clinical Oncology》 2026年第5期153-164,共12页
BACKGROUND Osteosarcoma(OS)is a primary solid tumor of bone.Due to the insidious onset and malignancy of OS,it is necessary to diagnose OS earlier.AIM To search for an optimal diagnostic biomarker,such as autoantibody... BACKGROUND Osteosarcoma(OS)is a primary solid tumor of bone.Due to the insidious onset and malignancy of OS,it is necessary to diagnose OS earlier.AIM To search for an optimal diagnostic biomarker,such as autoantibody against tumor associated antigen(TAA),for early diagnosis of OS.METHODS Differential expression of biomarkers in sera from 28 patients with OS and 49 normal human sera were screened by a focused protein microarray with 154 human recombinant proteins.Enzyme-linked immunosorbent assay(ELISA)was applied to validate the three potential biomarkers from 49 OS patients matched with 49 normal controls and 39 osteochondroma.The diagnostic value of each TAA for OS was analyzed by receiver operating characteristic curve.Western blotting and immunohistochemistry were used to verify the results of ELISA.RESULTS Based on the protein microarray,three differentially expressed TAAs[G-protein subunit alpha 11,serine/arginine-rich splicing factor 2(SRSF2),and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha]were screened out,whose content was higher than in normal human sera.According to the results of ELISA,anti-SRSF2 autoantibody showed higher expression in OS than in normal controls,with sensitivity,specificity,and area under the curve of 16.33%,95.92%,and 0.648,respectively.The ELISA results were confirmed by western blotting and immunohistochemistry.CONCLUSION Autoantibody is potential serological biomarker in detecting OS.Expression of anti-SRSF2 autoantibody has the potential to distinguish OS from the normal individuals. 展开更多
关键词 Osteosarcoma Tumor associated antigen Protein microarray Detection SRSF2 Biomarker
暂未订购 下载PDF
Identification of p63 expression in human lung cancer: analysis by complementary DNA and tissue microarray 认领 引用
2
作者 余永伟 Mitch Garber +2 位作者 Karsten Schlüns Manuela Pacyna-Gengelbach lver Petersen 《Journal of Medical Colleges of PLA(China)》 2004年第1期51-54,共4页
Objective: To evaluate p63 expression at mRNA transcripts and protein levels in lung squamous cell cancer (SCC), adenocarcinoma, large cell lung cancer (LCLC) and small cell lung cancer (SCLC) and their matched metast... Objective: To evaluate p63 expression at mRNA transcripts and protein levels in lung squamous cell cancer (SCC), adenocarcinoma, large cell lung cancer (LCLC) and small cell lung cancer (SCLC) and their matched metastatic tumors. The association between p63 expression and p63 locus at chromosomal 3q27 q29 was also investigated. Methods: p63 mRNA expression levels in a large series of lung cancers including SCC, adenocarcinoma, LCLC, SCLC and their matched metastatic tumors were analyzed by cDNA microarray technology. A tissue microarray from 150 primary lung cancer specimens was constructed and used for immunohistochemical detection of p63 protein expression. Chromosomal imbalances at the p63 locus in 70 primary lung cancers samples were studied by comparative genomic hybridization (CGH) technology. Results: mRNA levels were 10 fold in SCC compared to LCLC, SCLC, and adenocarcinoma. Interestingly, the mRNA expression of p63 in metastatic carcinomas was significantly higher than that in their matched primary tumors ( P <0 001). Immunohistochemistry demonstrated that p63 expression was 94.64% in SCC but only 1 79% in lung adenocarcinoma and 2 of 4 LCLC were positive staining. All the results in of SCLC were negative. There was a statistically significant difference for p63 positivity between pT1 tumors and those of higher stage ( P =0 035). The CGH results indicated that p63 locus at chromosomal 3q27 q29 was overrepresented in SCC. p63 immunopositivity correlated significantly with pronounced gains of the p63 locus at chromosomal 3q27 q29 (P=0.0001), indicating that strong expression of p63 in lung SCC correlated with increased gene amplification. Conclusion: p63 might play an important role not only in squamous differentiation of lung cancer but also in tumor development and progression. 展开更多
关键词 lung cancer cDNA microarray tissue microarray p63, comparative genomic hybridization
暂未订购 下载PDF
COX-2 expression in gastric cancer and its relationship with angiogenesis using tissue microarray 认领 引用 被引量：49
3
作者 Xiao-Yun Mao Xiao-Ge Wang +2 位作者 Xiao-Jun Lv Lei Xu Cheng-Bo Han 《World Journal of Gastroenterology》 SCIE CAS 2007年第25期3466-3471,共6页
AIM: To explore the expression and clinicopathological significance of cyclooxygenase-2 (COX-2) and microvessel density (MVD) in gastric carcinogenesis, and to investigate their roles in the invasion and the relations... AIM: To explore the expression and clinicopathological significance of cyclooxygenase-2 (COX-2) and microvessel density (MVD) in gastric carcinogenesis, and to investigate their roles in the invasion and the relationship between biological behaviors and prognosis of gastric cancer. METHODS: Using Envision immunohistochemistry, COX-2 and CD34 expressions in gastric cancer tissue array were examined. MVD was counted and the relationship between the biological behaviors and prognosis was analyzed. RESULTS: The expression of COX-2 in gastric cancer tissue was significantly higher than that in normal mucosa (χ2 = 12.191, P < 0.05). The over-expression of COX-2 in gastric cancer was obviously related to metastasis and depth of invasion (χ2 = 6.315, P < 0.05), but not related to the histological type and Borrmann type (χ2 = 5.391 and χ2 = 2.228, respectively). Moreover, MVD in gastric cancer tissues was significantly higher than that in the normal mucosa (65.49 ± 20.64 vs 36.21 ± 18.47, t/F = 7.53, P < 0. 05). MVD was related to the histologic type and metastasis (t/F = 3.68 and t/F = 4.214, respectively, P < 0. 05), but not related to the depth of invasion and Borrmann type (t/F = 0.583 and t/F = 0.459, respectively). MVD in COX-2-positive tissues was markedly higher compared to COX-2-negative tissues, indicating a positive correlation between COX-2 expression and MVD (t = 13.12, P < 0. 05). CONCLUSION: Tissue microarray (TMA) is a powerful tool for rapid identifi cation of the molecular alterations in gastric cancer. COX-2 expression, via inducingangiogenesis, may play an important role in gastric carcinogenesis. It could be served as a determinant factor for clinical prognosis and curative effect. 展开更多
关键词 Gastric cancer Tissue microarray COX-2 Immunohistochemistry CD34 Microvessel density
暂未订购 下载PDF
Salt-responsive genes in rice revealed by cDNA microarray analysis 认领 引用 被引量：20
4
作者 Dai Yin CHAO Yong Hai LUO +2 位作者 Min SHI Da LUO Hong Xuan LIN 《Cell Research》 SCIE CAS 2005年第10期796-810,共15页
We used cDNA microarrays containing ～9,000 unigenes to identify 486 salt responsive expressed sequence tags (ESTs) (representing ～450 unigenes) in shoots of the highly salt-tolerant rice variety, Nona Bokra (Oryza s... We used cDNA microarrays containing ～9,000 unigenes to identify 486 salt responsive expressed sequence tags (ESTs) (representing ～450 unigenes) in shoots of the highly salt-tolerant rice variety, Nona Bokra (Oryza sativa L. Ssp.Indica pv. Nona). Some of the genes identified in this study had previously been associated with salt stress. Howeverthe majority were novel, indicating that there is a great number of genes that are induced by salt exposure. Analysis of the salt stress expression profile data of Nona provided clues regarding some putative cellular and molecular processes that are undertaken by this tolerant rice variety in response to salt stress. Namely, we found that multiple transcription factors were induced during the initial salt response of shoots. Many genes whose encoded proteins are implicated in detoxification, protectant and transport were rapidly induced. Genes supporting photosynthesis were repressed and those supporting carbohydrate metabolism were altered. Commonality among the genes induced by salt exposure with those induced during senescence and biotic stress responses suggests that there are shared signaling pathways among these processes. We further compared the transcriptome changes of the salt-sensitive cultivar, IR28, with that of Nona rice. Many genes that are salt responsive in Nona were found to be differentially regulated in IR28. This study identified a large number of candidate functional genes that appear to be involved in salt tolerance and further examination of these genes may enable the molecular basis of salt tolerance to be elucidated. 展开更多
关键词 cDNA microarray rice salt stress signaling crosstalk transcriptome.
暂未订购 下载PDF
Detection and identification of Vibrio parahaemolyticus by multiplex PCR and DNA-DNA hybridization on a microarray 认领 引用 被引量：16
5
作者 Rongzhi Wang Jiadong Huang +6 位作者 Wei Zhang Guangmei Lin Junwei Lian Libin Jiang Hongcong Lin Songfa Wang Shihua Wang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2011年第3期129-135,共7页
In this paper, we developed a rapid and accurate method for the detection of Vibrio parahaemolyticus strains, using multiplex PCR and DNA--DNA hybridization. Multiplex PCR was used to simultaneously amplify three diag... In this paper, we developed a rapid and accurate method for the detection of Vibrio parahaemolyticus strains, using multiplex PCR and DNA--DNA hybridization. Multiplex PCR was used to simultaneously amplify three diagnostic genes （tlh, tdh andfla） that serve as molecular markers of V. parahaemolyticus. Biotinylated PCR products were hybridized to primers immobilized on a microarray, and detected by chemiluminesce with avidin-conjugated alkaline phosphatase. With this method, forty-five samples were tested. Eight known virulent strains （tlh＋dh＋/fla＋） and four known avirulent strains （tlh＋dh /fla＋） of the V. parahaemolyticus were successfully detected, and no non-specific hybridization and cross-hybridization reaction were found from fifteen closely-related strains （tlh-dh-/fla＋） of the Vibrio spp. In addition, all the other eighteen strains of non-Vibrio bacteria （tlh-dh /fla-） gave negative results. The DNA microarray successfully distinguished V. parahaemolyticus from other Vibrio spp. The results demonstrated that this was an efficient and robust method for identifying virulent strains of V. parahaemolyticus. 展开更多
关键词 Vibrio parahaemolyticus Multiplex PCR Hybridization Gene microarray
暂未订购 下载PDF
Identification of Annexin A1 protein expression in human gastric adenocarcinoma using proteomics and tissue microarray 认领 引用 被引量：9
6
作者 Zhi-Qiang Zhang Xiu-Juan Li +3 位作者 Gui-Tao Liu Xiang-Yang Zhang Yu Xia Hao Wen 《World Journal of Gastroenterology》 SCIE CAS 2013年第43期7795-7803,共9页
AIM:To study the differential expression of Annexin A1(ANXA1)protein in human gastric adenocarcinoma.This study was also designed to analyze the relationship between ANXA1 expression and the clinicopathological parame... AIM:To study the differential expression of Annexin A1(ANXA1)protein in human gastric adenocarcinoma.This study was also designed to analyze the relationship between ANXA1 expression and the clinicopathological parameters of gastric carcinoma.METHODS:Purified gastric adenocarcinoma cells(GAC)and normal gastric epithelial cells(NGEC)were obtained from 15 patients with gastric cancer by laser capture microdissection.All of the peptide specimens were labeled as18O/16O after trypsin digestion.Differential protein expressions were quantitatively identified between GAC and NGEC by nanoliter-reverse-phase liquid chromatography-mass/mass spectrometry(nanoRPLC-MS/MS).The expressions of ANXA1 in GAC and NGEC were verified by western blot analysis.The tissue microarray containing the expressed ANXA1 in 75 pairs of gastric carcinoma and paracarcinoma specimens was detected by immunohistochemistry(IHC).The relationship between ANXA1 expression and clinicopathological parametes of gastric carcinoma was analyzed.RESULTS:A total of 78 differential proteins were identified.Western blotting revealed that ANXA1 expression was significantly upregulated in GAC(2.17/1,P0.05).CONCLUSION:The upregulated ANXA1 expression may be associated with carcinogenesis,progression,invasion and metastasis of GAC.This protein could be considered as a biomarker of clinical prognostic prediction and targeted therapy of GAC. 展开更多
关键词 Gastric cancer Annexin A1 protein Proteomics Tissue microarray Immunohistochemistry
暂未订购 下载PDF
Microarray microRNA profiling of urinary exosomes in a 5XFAD mouse model of Alzheimer’s disease 认领 引用 被引量：10
7
作者 Zhiqi Song Yajin Qu +8 位作者 Yanfeng Xu Ling Zhang Li Zhou Yunlin Han Wenjie Zhao Pin Yu Yu Zhang Xianglei Li Chuan Qin 《Animal Models and Experimental Medicine》 CAS CSCD 2021年第3期233-242,共10页
Background:Alzheimer's disease(AD)is an incurable and irreversible neurodegen-erative disease,without a clear pathogenesis.Therefore,identification of candidates before amyloid-βplaque(Aβ)deposition proceeds is ... Background:Alzheimer's disease(AD)is an incurable and irreversible neurodegen-erative disease,without a clear pathogenesis.Therefore,identification of candidates before amyloid-βplaque(Aβ)deposition proceeds is of major significance for earlier intervention in AD.Methods:To explore the potential noninvasive earlier biomarkers of AD in a 5XFAD mouse model,microRNAs(miRNAs)from urinary exosomes in 1-month-old pre-Aβaccumulation 5XFAD mice models and their littermate controls were profiled by mi-croarray analysis.The differentially expressed miRNAs were further analyzed via droplet digital PCR(ddPCR).Results:Microarray analysis demonstrated that 48 differentially expressed miRNAs(18 upregulated and 30 downregulated),of which six miRNAs-miR-196b-5p,miR-339-3p,miR-34a-5p,miR-376b-3p,miR-677-5p,and miR-721-were predicted to display gene targets and important signaling pathways closely associated with AD pathogenesis and verified by ddPCR.Conclusions:Urinary exosomal miRNAs showing differences in expression prior to Aβ-plaque deposition were identified.These exosomal miRNAs represent potential noninvasive biomarkers that may be used to prevent AD in clinical applications. 展开更多
关键词 5XFAD mouse model Alzheimer's disease biomarkers microarray miRNA urinary exosome
暂未订购 下载PDF
Screening of Serum Biomarkers for Distinguishing between Latent and Active Tuberculosis Using Proteome Microarray 认领 引用 被引量：10
8
作者 CAO Shu Hui CHEN Yan Qing +4 位作者 SUN Yong LIU Yang ZHENG Su Hua ZHANG Zhi Guo LI Chuan You 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2018年第7期515-526,共12页
Objective To identify potential serum biomarkers for distinguishing between latent tuberculosis infection（LTBI） and active tuberculosis（TB）. Methods A proteome microarray containing 4,262 antigens was used for scr... Objective To identify potential serum biomarkers for distinguishing between latent tuberculosis infection（LTBI） and active tuberculosis（TB）. Methods A proteome microarray containing 4,262 antigens was used for screening serum biomarkers of 40 serum samples from patients with LTBI and active TB at the systems level. The interaction network and functional classification of differentially expressed antigens were analyzed using STRING 10.0 and the TB database, respectively. Enzyme-linked immunosorbent assays（ELISA） were used to validate candidate antigens further using 279 samples. The diagnostic performances of candidate antigens were evaluated by receiver operating characteristic curve（ROC） analysis. Both antigen combination and logistic regression analysis were used to improve diagnostic ability. Results Microarray results showed that levels of 152 Mycobacterium tuberculosis（Mtb）-antigenspecific IgG were significantly higher in active TB patients than in LTBI patients（P 〈 0.05）, and these differentially expressed antigens showed stronger associations with each other and were involved in various biological processes. Eleven candidate antigens were further validated using ELISA and showed consistent results in microarray analysis. ROC analysis showed that antigens Rv2031 c, Rv1408, and Rv2421 c had higher areas under the curve（AUCs） of 0.8520, 0.8152, and 0.7970, respectively. In addition, both antigen combination and logistic regression analysis improved the diagnostic ability. Conclusion Several antigens have the potential to serve as serum biomarkers for discrimination between LTBI and active TB. 展开更多
关键词 LTBI Active TB Proteome microarray Serum biomarkers
暂未订购 下载PDF
Comparison of gene expression profiles between primary tumor and metastatic lesions in gastric cancer patients using laser microdissection and cDNA microarray 认领 引用 被引量：9
9
作者 Long Wang Jin-Shui Zhu +2 位作者 Ming-Quan Song Guo-Qiang Chen Jin-Lian Chen 《World Journal of Gastroenterology》 SCIE CAS 2006年第43期6949-6954,共6页
AIM. To study the differential gene expression profiles of target cells in primary gastric cancer and its metastatic lymph nodes using laser microdissection （LMD） in combination with cDNA microarray. METHODS： Norma... AIM. To study the differential gene expression profiles of target cells in primary gastric cancer and its metastatic lymph nodes using laser microdissection （LMD） in combination with cDNA microarray. METHODS： Normal gastric tissue samples from 30 healthy individuals, 36 cancer tissue samples from primary gastric carcinoma and lymph node metastasis tissue samples from 58 patients during gastric cancer resection were obtained using LMD in combination with cDNA microarray independently. After P27-based amplification, aRNA from 36 of 58 patients （group 1） with lymph node metastasis and metastatic tissue specimens from the remaining 22 patients （group 2） were applied to cDNA microarray. Semiquantitative reverse transcriptase polymerase chain reaction （RT-PCR） and imrnunohistochemical assay verified the results of microarray in group 2 and further identified genes differentially expressed in the progression of gastric cancer. RESULTS： The expression of 10 genes was up-regulated while the expression of 15 genes was down-regulated in 22 gastric carcinoma samples compared with that of genes in the normal controls. The results were confirmed at the level of mRNA and protein, and suggested that four genes （OPCML, RNASE1, YES1 and ACK1） could play a key role in the tumorigenesis and metastasis of gastric cancer. The expression pattern of 3 genes （OPCML, RNASE1 and YES1） was similar to tumor suppressor genes. For example, the expression level of these genes was the highest in normal gastric epithelium, which was decreased in primary carcinoma, and further decreased in metastatic lymph nodes. On the contrary, the expression pattern of gene ACK1 was similar to that of oncogene. Four genes were further identified as differentially expressed genes in the majority of the cases in the progression of gastric cancer. CONCLUSION： LMD in combination with cDNA microaro ray provides a unique support foe the identification of early expression profiles of differential genes and the expression pattern of 3 genes （OPCML, RNASE1 and YES1） associated with the progression of gastric cancer. Further study is needed to reveal the molecular mechanism of lymph node metastasis in patients with gastric cancer. 展开更多
关键词 Gastric cancer cDNA microarray Laser microdissection Reverse transcriptase polymerase chain reaction P27
暂未订购 下载PDF
Clinical Application of Chromosome Microarray Analysis in Han Chinese Children with Neurodevelopmental Disorders 认领 引用 被引量：10
10
作者 Mingyu Xu Yiting Ji +4 位作者 Ting Zhang Xiaodong Jiang Yun Fan Juan Geng Fei Li 《Neuroscience Bulletin》 SCIE CAS CSCD 2018年第6期981-991,共11页
Chromosome microarray analysis(CMA) is a cost-effective molecular cytogenetic technique that has been used as a first-line diagnostic test in neurodevelopmental disorders in the USA since 2011. The impact of CMA resul... Chromosome microarray analysis(CMA) is a cost-effective molecular cytogenetic technique that has been used as a first-line diagnostic test in neurodevelopmental disorders in the USA since 2011. The impact of CMA results on clinical practice in China is not yet well studied, so we aimed to better evaluate this phenomenon.We analyzed the CMA results from 434 patients in our clinic, and characterized their molecular diagnoses, clinical features, and follow-up clinical actions based on these results. The overall diagnostic yield for our patients was 13.6%(59 out of 434). This gave a detection rate of 14.7%for developmental delay/intellectual disability(DD/ID,38/259) and 12% for autism spectrum disorders(ASDs,21/175). Thirty-three recurrent(n≥2) variants were found, distributed at six chromosomal loci involving known chromosome syndromes(such as DiGeorge, Williams Beuren, and Angelman/Prader-Willi syndromes).The spectrum of positive copy number variants in our study was comparable to that reported in Caucasian populations, but with specific characteristics. Parental origin tests indicated an effect involving a significant maternal transmission bias to sons. The majority of patients with positive results(94.9%) had benefits, allowing earlier diagnosis(36/59), prioritized full clinical management(28/59), medication changes(7/59), a changed prognosis(30/59), and prenatal genetic counseling(15/59). Our results provide information on de novo mutations in Chinese children with DD/ID and/or ASDs. Our data showed that microarray testing provides immediate clinical utility for patients. It is expected that the personalized medical care of children with developmental disabilities will lead to improved outcomes in long-term developmental potential.We advocate using the diagnostic yield of clinically actionable results to evaluate CMA as it provides information of both clinical validity and clinical utility. 展开更多
关键词 Chromosome microarray analysis Neurode velopmental disorder Autism spectrum disorder Chro mosome syndrome Clinical management
暂未订购 下载PDF
Detection of Genetically Modified Crops by Combination of Multiplex PCR and Low-density DNA Microarray 认领 引用 被引量：15
11
作者 PING-PING ZHOU JIAN-ZHONG ZHANG +1 位作者 YUAN-HAI YOU YONG-NING WU 《Biomedical and Environmental Sciences》 SCIE CAS 2008年第1期53-62,共10页
Objective To develop a technique for simultaneous detection of various target genes in Roundup Ready soybean by combining multiplex PCR and low-density DNA microarray. Methods Two sets of the multiplex PCR system were... Objective To develop a technique for simultaneous detection of various target genes in Roundup Ready soybean by combining multiplex PCR and low-density DNA microarray. Methods Two sets of the multiplex PCR system were used to amplify the target genes in genetically modified （GM） soybean. Seventeen capture probes （PCR products） and 17 pairs of corresponding primers were designed according to the genetic characteristics of Rroundup Ready soybean （GTS40-3-2）, maize （MonS10, Nk603, GA21）, canola （T45, MS1/RF1）, and rice （SCK） in many identified GM crops. All of the probes were categorized and identified as species-specific probes. One negative probe and one positive control probe were used to assess the efficiency of all reactions, and therefore eliminate any false positive and negative results. After multiplex PCR reaction, amplicons were adulterated with Cy5-dUTP and hybridized with DNA microarray. The array was then scanned to display the specific hybridization signals of target genes. The assay was applied to the analysis of sample of certified transgenic soybean （Roundup Ready GTS40-3-2） and canola （MS1/RF1）. Results A combination technique of multiplex PCR and DNA microarray was successfully developed to identify multi-target genes in Roundup Ready soybean and MS 1/RF1 canola with a great specificity and reliability. Reliable identification of genetic characteristics of Roundup Ready of GM soybean from genetically modified crops was achieved at 0.5% transgenic events, indicating a high sensitivity. Conclusion A combination technique of multiplex PCR and low-density DNA microarray can reliably detect and identify the genetically modified crops. 展开更多
关键词 Genetically modified organisms Low-density DNA microarray Multiplex PCR Roundup Ready soybean MS 1/RF1 canola
暂未订购 下载PDF
Novel circular RNA expression profile of uveal melanoma revealed by microarray 认领 引用 被引量：8
12
作者 Xuan Yang Yang Li +6 位作者 Yueming Liu Xiaolin Xu Yingzhi Wang Yanni Yan Wenjia Zhou Jingyan Yang Wenbin Wei 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2018年第6期656-668,共13页
Objective: The present study aimed to investigate circular RNA(circRNA) expression in uveal melanoma(UM).Methods: First,we used microarray to compare the expression profiles of circRNA in five UM samples and five norm... Objective: The present study aimed to investigate circular RNA(circRNA) expression in uveal melanoma(UM).Methods: First,we used microarray to compare the expression profiles of circRNA in five UM samples and five normal uvea tissues.Next,bioinformatics analyses,including gene ontology(GO) analysis and pathway analysis,were applied to study these differentially expressed circRNAs to predict pathogenic pathways that may be involved.Quantitative real-time polymerase chain reaction(qRT-PCR) in 20 UM samples and 20 normal uvea samples was used to confirm the circRNA expression profiles obtained from the microarray data.Finally,we analyzed the interaction between validated circRNAs and their potential cancer-associated miRNA targets.Results: In total,50,579 circRNAs [fold change(FC) ≥2.0; P<0.05],including 20,654 up-regulated and 29,925 down-regulated circRNAs,were identified as differentially expressed between UM tissues and normal uvea tissues.We used qRT-PCR to verify seven dysregulated circRNAs indicated by the microarray data,including hsacirc0119873,hsacirc0128533,hsacirc0047924,hsacirc0103232,hsa-circRNA10628-6,hsacirc0032148 and hsacirc0133460,which may be promising candidates to study future molecular mechanisms.Conclusions: This study explored,for the first time,the abnormal expression of circRNAs in UM and described the expression profile of circRNAs,providing a new potential target for the mechanism of UM and future treatment of UM. 展开更多
关键词 Uveal melanoma circular RNA microarray noncoding RNA
暂未订购 下载PDF
Application of restriction display PCR technique in the preparation of cDNA microarray probes 认领 引用 被引量：7
13
作者 Zhao-Hui Sun Wen-Li Ma +2 位作者 Bao Zhang Yi-Fei Peng Wen-Ling Zheng 《World Journal of Gastroenterology》 SCIE CAS 2005年第48期7579-7584,共6页
AIM: To develop a simplified and efficient method for the preparation of hepatitis C virus (HCV) cDNA microarray probes.METHODS: With the technique of restriction display PCR (RD-PCR), restriction enzyme Sau3A I was c... AIM: To develop a simplified and efficient method for the preparation of hepatitis C virus (HCV) cDNA microarray probes.METHODS: With the technique of restriction display PCR (RD-PCR), restriction enzyme Sau3A I was chosen to digest the full-length HCV cDNAs. The products were classified and re-amplified by RD-PCR. We separated the differential genes by polyacrylamide gel electrophoresis and silver staining. Single bands cut out from the polyacrylamide gel were isolated. The third-round PCR was performed using the single bands as PCR template.The RD-PCR fragments were purified and cloned into the pMD18-T vector. The recombinant plasmids were extracted from positive clones, and the target gene fragments were sequenced. The cDNA microarray was prepared by spotting RD-PCR products to the surface of amino-modified glass slides using a robot. We validated the detection of microarray by hybridization and sequence analysis.RESULTS: A total of 24 different cDNA fragments ranging from 200 to 800 bp were isolated and sequenced,which were the specific gene fragments of HCV. These fragments could be further used as probes in microarray preparation. The diagnostic capability of the microarray was evaluated after the washing and scanning steps. The results of hybridization and sequence analysis showed that the specificity, sensitivity, accuracy, reproducibility,and linearity in detecting HCV RNA were satisfactory.CONCLUSION: The RD-PCR technique is of great value in obtaining a large number of size-comparable gene probes, which provides a speedy protocol in generating probes for the preparation of microarrays. Microarray prepared as such could be further optimized and applied in the clinical diagnosis of HCV. 展开更多
关键词 Restriction display PCR HCV Microarray Probes
暂未订购 下载PDF
Rapid Detection of rpoB Gene Mutations in Rif-resistant M.tuberculosis Isolates by Oligonucleotide Microarray 认领 引用 被引量：9
14
作者 AI-HUA SUN XING-LI FAN +3 位作者 LI-WEI LI LI-FANG WANG WEN-YING AN JIE YAN 《Biomedical and Environmental Sciences》 SCIE CAS 2009年第3期253-258,共6页
Objective To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray. Methods Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DN... Objective To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray. Methods Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray Results Of the 102 rifampin-resistant strains 98 （96.1%） had mutations in the rpoB genes. Conclusion Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates. 展开更多
关键词 Mycobacterium tuberculosis Rifampin resistance rpoB gene / site mutation Oligonucleotide microarray/detection
暂未订购 下载PDF
Microarray Analysis of Gene Expression Changes in Neuroplastin 65-Knockout Mice: Implications for Abnormal Cognition and Emotional Disorders 认领 引用 被引量：6
15
作者 Huanhuan Li Jiujiang Zeng +4 位作者 Liang Huang Dandan Wu Lifen Liu Yutong Liu Qionglan Yuan 《Neuroscience Bulletin》 SCIE CAS CSCD 2018年第5期779-788,共10页
Neuroplastin 65 （Np65） is an immunoglobulin superfamily cell adhesion molecule involved in synaptic formation and plasticity. Our recent study showed that Np65-knockout （KO） mice exhibit abnormal cognition and emo... Neuroplastin 65 （Np65） is an immunoglobulin superfamily cell adhesion molecule involved in synaptic formation and plasticity. Our recent study showed that Np65-knockout （KO） mice exhibit abnormal cognition and emotional disorders. However, the underlying mechanisms remain unclear. In this study, we found 588 differentially- expressed genes in Np65-KO mice by microarray analysis. RT-PCR analysis also revealed the altered expression of genes associated with development and synaptic structure, such as Cdhl, Htr3a, and Kcnj9. In addition, the expression of Wnt-3, a Wnt protein involved in development, was decreased in Np65-KO mice as evidenced by western blotting. Surprisingly, MRI and DAPI staining showed a significant reduction in the lateral ventricular volume of Np65-KO mice. Together, these findings suggest that ablation of Np65 influences gene expression, which may contribute to abnormal brain development. These results provide clues to the mechanisms underlying the altered brain functions of Np65-deficient mice. 展开更多
关键词 Neuroplastin 65 Microarray analysis Gene expression profile Htr3a Wnt
暂未订购 下载PDF
Bioinformatics analyses of differentially expressed genes associated with spinal cord injury:a microarray-based analysis in a mouse model 认领 引用 被引量：5
16
作者 Lei Guo Jing Lv +2 位作者 Yun-Fei Huang Ding-Jun Hao Ji-Jun Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第7期1262-1270,共9页
Gene spectrum analysis has shown that gene expression and signaling pathways change dramatically after spinal cord injury,which may affect the microenvironment of the damaged site.Microarray analysis provides a new op... Gene spectrum analysis has shown that gene expression and signaling pathways change dramatically after spinal cord injury,which may affect the microenvironment of the damaged site.Microarray analysis provides a new opportunity for investigating diagnosis,treatment,and prognosis of spinal cord injury.However,differentially expressed genes are not consistent among studies,and many key genes and signaling pathways have not yet been accurately studied.GSE5296 was retrieved from the Gene Expression Omnibus DataSet.Differentially expressed genes were obtained using R/Bioconductor software(expression changed at least two-fold;P < 0.05).Database for Annotation,Visualization and Integrated Discovery was used for functional annotation of differentially expressed genes and Animal Transcription Factor Database for predicting potential transcription factors.The resulting transcription regulatory protein interaction network was mapped to screen representative genes and investigate their diagnostic and therapeutic value for disease.In total,this study identified 109 genes that were upregulated and 30 that were downregulated at 0.5,4,and 24 hours,and 3,7,and 28 days after spinal cord injury.The number of downregulated genes was smaller than the number of upregulated genes at each time point.Database for Annotation,Visualization and Integrated Discovery analysis found that many inflammation-related pathways were upregulated in injured spinal cord.Additionally,expression levels of these inflammation-related genes were maintained for at least 28 days.Moreover,399 regulation modes and 77 nodes were shown in the protein-protein interaction network of upregulated differentially expressed genes.Among the 10 upregulated differentially expressed genes with the highest degrees of distribution,six genes were transcription factors.Among these transcription factors,ATF3 showed the greatest change.ATF3 was upregulated within 30 minutes,and its expression levels remained high at28 days after spinal cord injury.These key genes screened by bioinformatics tools can be used as biological markers to diagnose diseases and provide a reference for identifying therapeutic targets. 展开更多
关键词 nerve regeneration spinal cord injury differentially expressed genes bioinformatics analyses Database for Annotation,Visualization and Integrated Discovery analysis inflammation Kyoto Encyclopedia of Genes and Genomes pathway microarray transcription factors neural regeneration
暂未订购 下载PDF
Protein microarray analysis of cytokine expression changes in distal stumps after sciatic nerve transection 认领 引用 被引量：5
17
作者 Xiao-Qing Cheng Xue-Zhen Liang +9 位作者 Shuai Wei Xiao Ding Gong-Hai Han Ping Liu Xun Sun Qi Quan He Tang Qing Zhao Ai-Jia Shang Jiang Peng 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第3期503-511,共9页
A large number of chemokines,cytokines,other trophic factors and the extracellular matrix molecules form a favorable microenvironment for peripheral nerve regeneration.This microenvironment is one of the major factors... A large number of chemokines,cytokines,other trophic factors and the extracellular matrix molecules form a favorable microenvironment for peripheral nerve regeneration.This microenvironment is one of the major factors for regenerative success.Therefore,it is important to investigate the key molecules and regulators affecting nerve regeneration after peripheral nerve injury.However,the identities of specific cytokines at various time points after sciatic nerve injury have not been determined.The study was performed by transecting the sciatic nerve to establish a model of peripheral nerve injury and to analyze,by protein microarray,the expression of different cytokines in the distal nerve after injury.Results showed a large number of cytokines were up-regulated at different time points post injury and several cytokines,e.g.,ciliary neurotrophic factor,were downregulated.The construction of a protein-protein interaction network was used to screen how the proteins interacted with differentially expressed cytokines.Kyoto Encyclopedia of Genes and Genomes pathway and Gene ontology analyses indicated that the differentially expressed cytokines were significantly associated with chemokine signaling pathways,Janus kinase/signal transducers and activators of transcription,phosphoinositide 3-kinase/protein kinase B,and notch signaling pathway.The cytokines involved in inflammation,immune response and cell chemotaxis were up-regulated initially and the cytokines involved in neuronal apoptotic processes,cell-cell adhesion,and cell proliferation were up-regulated at 28 days after injury.Western blot analysis showed that the expression and changes of hepatocyte growth factor,glial cell line-derived neurotrophic factor and ciliary neurotrophic factor were consistent with the results of protein microarray analysis.The results provide a comprehensive understanding of changes in cytokine expression and changes in these cytokines and classical signaling pathways and biological functions during Wallerian degeneration,as well as a basis for potential treatments of peripheral nerve injury.The study was approved by the Institutional Animal Care and Use Committee of the Chinese PLA General Hospital,China(approval number:2016-x9-07)in September 2016. 展开更多
关键词 cytokines distal stump gene ontology Kyoto Encyclopedia of Genes and Genomes pathway peripheral nerve injury protein microarray protein-protein interaction network Wallerian degeneration
暂未订购 下载PDF
Analysis of gene expression profiles in pancreatic carcinoma by using cDNA microarray 认领 引用 被引量：11
18
作者 Xian-Jun Yu Jiang Long +2 位作者 De-Liang Fu Qun-Hua Zhang Quan-Xin Ni the Center for Pancreatic Cancer, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China 《Hepatobiliary & Pancreatic Diseases International》 CAS 2003年第3期467-470,共4页
OBJECTIVES: To survey the gene expression profiles in pancreatic carcinoma by using cDNA microarray and detect target genes for further study. METHODS: Three mixed samples from 2 cases of normal pancreatic tissue and ... OBJECTIVES: To survey the gene expression profiles in pancreatic carcinoma by using cDNA microarray and detect target genes for further study. METHODS: Three mixed samples from 2 cases of normal pancreatic tissue and 4 cases of moderate-differentiated pancreatic carcinoma were studied by means of cDNA microarray consisting of 18 000 genes. RESULTS: 1484 and 1353 different expressed genes were observed in two cancer samples respectively. We identified 455 genes altered with the same tendency in both samples, including 102 up-regulated and 353 down-regulated genes. There were 274 known genes and 181 unknown genes; 27.8% and 52.0% genes respectively had an expression level in cancer that was 2-fold higher or lower than that in normal samples. Tumor suppressor genes, growth factors and receptor genes, signal conduction genes, transcription factor genes were identified. CONCLUSIONS: cDNA microarray is an efficient and high-throughout method to investigate gene expression profiles in pancreatic carcinoma. MBD1, EDG1 and gene hypermethylation mechanism would play an important role in the pathogenesis of pancreatic carcinoma. 展开更多
关键词 pancreatic carcinoma cDNA microarray gene expression profiles
暂未订购 下载PDF
Plasma long noncoding RNA expression profile identified by microarray in patients with Crohn's disease 认领 引用 被引量：6
19
作者 Dong Chen Jiang Liu +3 位作者 Hui-Ying Zhao Yi-Peng Chen Zun Xiang Xi Jin 《World Journal of Gastroenterology》 SCIE CAS 2016年第19期4716-4731,共16页
AIM: To investigate the expression pattern of plasma long noncoding RNAs (lncRNAs) in Chrohn&#x02019;s disease (CD) patients.METHODS: Microarray screening and qRT-PCR verification of lncRNAs and mRNAs were perform... AIM: To investigate the expression pattern of plasma long noncoding RNAs (lncRNAs) in Chrohn&#x02019;s disease (CD) patients.METHODS: Microarray screening and qRT-PCR verification of lncRNAs and mRNAs were performed in CD and control subjects, followed by hierarchy clustering, GO and KEGG pathway analyses. Significantly dysregulated lncRNAs were categorized into subgroups of antisense lncRNAs, enhancer lncRNAs and lincRNAs. To predict the regulatory effect of lncRNAs on mRNAs, a CNC network analysis was performed and cross linked with significantly changed lncRNAs. The overlapping lncRNAs were randomly selected and verified by qRT-PCR in a larger cohort.RESULTS: Initially, there were 1211 up-regulated and 777 down-regulated lncRNAs as well as 1020 up-regulated and 953 down-regulated mRNAs after microarray analysis; a heat map based on these results showed good categorization into the CD and control groups. GUSBP2 and {'type':'entrez-nucleotide','attrs':{'text':'AF113016','term_id':'6642755','term_text':'AF113016'}}AF113016 had the highest fold change of the up- and down-regulated lncRNAs, whereas TBC1D17 and CCL3L3 had the highest fold change of the up- and down-regulated mRNAs. Six (SNX1, CYFIP2, CD6, CMTM8, STAT4 and IGFBP7) of 10 mRNAs and 8 ({'type':'entrez-nucleotide','attrs':{'text':'NR_033913','term_id':'299890801','term_text':'NR_033913'}}NR_033913, {'type':'entrez-nucleotide','attrs':{'text':'NR_038218','term_id':'333360887','term_text':'NR_038218'}}NR_038218, {'type':'entrez-nucleotide','attrs':{'text':'NR_036512','term_id':'302393555','term_text':'NR_036512'}}NR_036512, {'type':'entrez-nucleotide','attrs':{'text':'NR_049759','term_id':'388240827','term_text':'NR_049759'}}NR_049759, {'type':'entrez-nucleotide','attrs':{'text':'NR_033951','term_id':'300068996','term_text':'NR_033951'}}NR_033951, {'type':'entrez-nucleotide','attrs':{'text':'NR_045408','term_id':'354548835','term_text':'NR_045408'}}NR_045408, {'type':'entrez-nucleotide','attrs':{'text':'NR_038377','term_id':'335334999','term_text':'NR_038377'}}NR_038377 and {'type':'entrez-nucleotide','attrs':{'text':'NR_039976','term_id':'337756399','term_text':'NR_039976'}}NR_039976) of 14 lncRNAs showed the same change trends on the microarray and qRT-PCR results with statistical significance. Based on the qRT-PCR verified mRNAs, 1358 potential lncRNAs with 2697 positive correlations and 2287 negative correlations were predicted by the CNC network.CONCLUSION: The plasma lncRNAs profiles provide preliminary data for the non-invasive diagnosis of CD and a resource for further specific lncRNA-mRNA pathway exploration. 展开更多
关键词 Crohn’ s disease Long noncoding RNA Inflammatory bowel disease Plasma Microarray
暂未订购 下载PDF
EXPRESSION AND SIGNIFICANCE OF SURVIVIN mRNA IN LUNG CANCER TISSUE MICROARRAY DETECTED BY FISH 认领 引用 被引量：7
20
作者 Xin-yun Wang Zhi Yao +7 位作者 Yan Li Ting Liu Hai-yan Zheng Cong-zhong Zhu Cui-yun Sun Ai-xiang Wang Min Zhao Xing-ye Wu 《Chinese Medical Sciences Journal》 CAS 2005年第3期214-216,共3页
Objective To investigate the expression of Survivin mRNA in lung cancer tissue microarray （TMA） by fluorescence in .situ hybridization （FISH） method, and determine the role and significance of it in lung cancer ge... Objective To investigate the expression of Survivin mRNA in lung cancer tissue microarray （TMA） by fluorescence in .situ hybridization （FISH） method, and determine the role and significance of it in lung cancer genesis and progress. Methods The expression of Survivin mRNA was detected by FISH method and TMA technology. Fifty-four cases of lung cancer and 10 cases of normal lung tissue were examined. Survivin mRNA was expressed in 66.7% （36/54） of lung cancer; the positive ratio of lung cancer was significantly higher than that of normal lung tissue （0/10;X^2= 15.238, P 〈 0.05）. The positive ratio of Survivin mRNA was significantly higher in poor differentiated cancer （20/24, 83.3% ） than moderate and well differentiated cancer （16/30, 53.3%; X^2 = 5.40, P 〈 0.05）. The positive ratio of Survivin mRNA was significantly higher in group with lymph node metastasis （27/32, 84.4%） than without lymph node metastasis （9/22, 40.9%; X^2= 11.084, P 〈 0.05）. The positive ratio of Survivin mRNA was significantly higher in stage Ⅲ-Ⅳ（12/13, 92.3%） than stage Ⅰ- Ⅱ （24/41,58.5%; X^2=5.066, P〈 0.05）. Conclusion Survivin mRNA highly expresses in lung cancer, which is related to the progress and malignant behavior. Survivin may play a promoting role in lung cancer genesis and progress and provide a basis for estimating prognosis and treatment. 展开更多
关键词 fluorescence in situ hybridization tissue microarray Survivin lung cancer
暂未订购 下载PDF
已选0条
引用分析
参考文献
引证文献
 统计分析
检索结果
已选文献
上一页 1 2 … 39 下一页 到第 页
在线咨询 使用帮助 返回顶部 意见反馈