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Effect of nano-selenium loaded with lycium barbarum polysaccharide on the proliferation of lens epithelial cells after UVB damage in vitro 认领 引用 被引量:3
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作者 Jing-Xiang Zhong Shan-Shan Jin +3 位作者 Kang-Sheng Wu Guo-Cheng Yu Lei-Lei Tu Lian Liu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2022年第1期9-14,共6页
AIM:To investigate the effect of nano-selenium loaded with different concentrations of lycium barbarum polysaccharide(LBP-Se NPs)on the proliferation of human lens epithelial cells(HLECs)from UV irradiation.METHODS:LB... AIM:To investigate the effect of nano-selenium loaded with different concentrations of lycium barbarum polysaccharide(LBP-Se NPs)on the proliferation of human lens epithelial cells(HLECs)from UV irradiation.METHODS:LBP-Se NPs were prepared and their particle size was detected.HLECs(SRA01/04)were irradiated with UVB for different time(0,10,20,30,40,50,60 min)to construct a damaged model,the survival rate of cells was determined by methylthiazol tetrazolium(MTT)assay.The 4’,6-Diamidine-2’-phenylindole dihydrochloride(DAPI)staining was used to observe the status of cell nucleus and drug entering cytoplasm through cell membrane in SRA01/04 cells after adding LBP-SENPS loaded with coumarin fluorescence agent 24 h under fluorescence microscope.SRA01/04 normal and UVB-damaged cells were treated with different amounts of LBP-Se NPs at different concentrations,cells proliferation were observed.RESULTS:The particle size of LBP-Se NPs was stable in the range of 150-200 nm.The survival rate changes with time after UVB irradiation were statistically significant.The 10 min of UVB exposure as the time was chosen to construct the cell damage model.With DAPI staining,LBPSe NPs were observed to enter the cytoplasm through the cell membrane under fluorescence inverted microscope.Cytotoxicity of SRA01/04 at different concentrations of LBPSe NPs were measured.Cell survival rate was statistically different compared with the control group.The higher the loading concentration of LBP in nano-Se drugs was,the higher the cell proliferation rate was(P<0.05).The lower the concentration of LBP-Se NPs,the higher the cell proliferation rate,showing a negative growth trend(P<0.05).The group with the highest average cell proliferation rate was 0.5μmol/L 2.0 mg/m L LBP-Se NPs(128.80%).When the 2.0 mg/m L LBP-Se NPs group was selected for cell photography,the cell density was higher at 0.5μmol/L.With the increase of concentration,SRA01/04 cells appeared more cytoplasm dehydration,cell shrinkage and apoptotic bodies,and cell density decreased.CONCLUSION:LBP-Se NPs has moderate particle size and good stability.LBP-Se NPs can protect HLECs(SRA01/04)from UVB-induced damage,and the cell proliferation rate is further increased with increasing the amount of loaded LBP and decreasing nano-selenium concentration. 展开更多
关键词 lycium barbarum polysaccharide nano-selenium human lens epithelial cells ultraviolet irradiation
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Effects of Different Application Methods and Application Rates of Biological Nano-Selenium on the Quality of Passion Fruit 认领 引用 被引量:7
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作者 Wei HUANG Minying SHEN +2 位作者 Zhaoliang LIU Yu ZHANG Daobo WANG 《Medicinal Plant》 2020年第3期68-69,92,共2页
[Objectives]To study the effects of different application methods and application rates of biological nano-selenium on the quality of passion fruit.[Methods]In this experiment,passion fruit Tainong 1 was sprayed with ... [Objectives]To study the effects of different application methods and application rates of biological nano-selenium on the quality of passion fruit.[Methods]In this experiment,passion fruit Tainong 1 was sprayed with biological nano-selenium.The concentration of spraying refers to the recommended application rate of biological nano-selenium manufacturers.Three treatments with different concentrations were carried out:3,4.5 and 6 L/ha biological nano-selenium was sprayed on 100%root,(50%root+50%leaf surface)and 100%leaf surface of passion fruit,respectively.[Results]From the aspects of economic cost and rational utilization of resources,spraying 3 L/ha selenium fertilizer on the leaves of passion fruit played the best role in improving the quality of passion fruit.[Conclusions]To study the selenium-rich production technology of agricultural products and improve the quality of agricultural products is of great significance for improving human health and producing high-quality agricultural products. 展开更多
关键词 Biological nano-selenium Fertilization method Passion fruit Quality
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Evaluation and Exploration of Se-enriching Effects of Microbial Nano-selenium on Several Kinds of Vegetable 认领 引用 被引量:1
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作者 Qiang LI Jing ZHANG +3 位作者 Ruiyi YANG Zujiang GAO Jiliang SHAN Puxing LIANG 《Agricultural Biotechnology》 2018年第5期26-28,共3页
[Objectives] This study was carried out to explore the selenium-enriching effect of different crops, and provide certain references for production of selenium-enriched agricultural products. [Methods] Four kinds of cr... [Objectives] This study was carried out to explore the selenium-enriching effect of different crops, and provide certain references for production of selenium-enriched agricultural products. [Methods] Four kinds of crops (tomato, leaf mustard, peanut, and carrot) were exogenous selenium fertilizer treated, to explore the selenium-enriching effects on these crops. [Results] Exogenous application of microbial nano-selenium can significantly increase the selenium content of the crops, of which the effects on big tomato (Weiyanuo) were the best. After spraying, the selenium content increased by 1.39 times, and at the same time the sugar content of tomato was also increased. [Conclusions] This study is expected to provide references for increase of the selenium content of agricultural products. 展开更多
关键词 Microbial nano-selenium Tomato Leaf mustard Peanut Carrot Selenium-enriching effects
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Antioxidant and Anti-Inflammatory Effects of Nano-Selenium against Cypermethrin-Induced Liver Toxicity 认领 引用
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作者 Rania H. Abdou Noha Sayed 《CellBio》 2019年第4期53-65,共13页
Cypermethrin (Cym) is a synthetic class II pyrethroid that is widely used and has a big risk to health. Cypermethrin produces oxidative stress and enhances inflammatory damage of liver. The present study was designed ... Cypermethrin (Cym) is a synthetic class II pyrethroid that is widely used and has a big risk to health. Cypermethrin produces oxidative stress and enhances inflammatory damage of liver. The present study was designed to investigate the ameliorating effects of NSe against Cym-induced hepatotoxicity in rats. For this purpose, twenty four male rats were divided into three groups. Group (I) was gavaged with Cym (control group), group (II) gavaged daily with Cym (1 mg/kg body weight), and group (III) gavaged with Cym + NSe (2.5 mg kg body weight/day, three times a week) for 21 days. Cypermethrin increased serum liver enzymes, oxidative stress and inflammatory markers. Administration of NSe significantly reduced the increased serum liver enzymes and inflammatory parameters and restored the antioxidant capacity in liver. Our results suggest that Nse exhibits promising hepato-protective effects against Cym-induced oxidative damage and inflammation. 展开更多
关键词 Cypermethrin Oxidative Stress Anti-Inflammatory Hepatoprotective Nano-Selenium
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Cornus officinalis polysaccharides nano-selenium exhibits anti- fatigue effects by regulating gut microbiota in mice 认领 引用 被引量:2
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作者 Jianrui Sun Ruirui Ren +6 位作者 Jinglan Li Jiaqi Fan Linlin Yao Shanshan Tie Jie Zhang Dahong Wang Shaobin Gu 《Food Bioscience》 SCIE 2025年第11期2090-2101,共12页
Cornus officinalis polysaccharides(CSP1)were isolated and purified,which were utilized as a modifier and stabilizer to prepare Cornus officinalis polysaccharides nano-selenium(CSP1-SeNPs).The structural properties of ... Cornus officinalis polysaccharides(CSP1)were isolated and purified,which were utilized as a modifier and stabilizer to prepare Cornus officinalis polysaccharides nano-selenium(CSP1-SeNPs).The structural properties of CSP1-SeNPs were characterized,and their anti-fatigue efficacy was evaluated.The successful preparation of CSP1-SeNPs was verified through material characterization,revealing a 96 nm mean particle size and superior stability.The maximum scavenging rates of CSP1-SeNPs on·DPPH,·OH,and·ABTS+free radicals were 96.5%,99.6%,and 94.7%.After 4 weeks of intervention,CSP1-SeNPs notably extended the exhaustive swimming duration in mice,markedly increased muscle and liver glycogen levels,and enhanced the activities of catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)(p<0.05).Additionally,CSP1-SeNPs significantly reduced the levels of blood urea nitrogen(BUN),blood lactic acid(BLA),creatine kinase(CK),lactate dehydrogenase(LDH),and malondialdehyde(MDA)(p<0.05).Furthermore,CSP1-SeNPs significantly increased the content of short-chain fatty acids(SCFAs)in mice feces.16S rRNA gene sequencing analysis revealed that CSP1-SeNPs reshaped the diversity and composition of the gut microbiota,significantly increasing the abundance of beneficial bacteria such as Roseburia,Bacteroides,Alloprevotella,Lachnospiraceae_uncultured,and Lactobacillus.In summary,CSP1-SeNPs could be utilized by the gut microbiota to exert anti-fatigue effects through promoting the proliferation of beneficial bacteria and the production of SCFAs.These findings provided a scientific foundation for considering CSP1-SeNPs as a potential prebiotic in the development of anti-fatigue functional foods. 展开更多
关键词 Cornus officinalis Polysaccharides Nano-selenium Anti-fatigue Gut microbiota
在饲粮中添加纳米硒对产蛋后期蛋鸡生产性能、蛋品质及抗氧化指标的影响 认领 引用 被引量:2
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作者 李佳博 马巍 +5 位作者 王兴 栗铭谦 常帅 罗冰 刘娜 王春强 《中国饲料》 北大核心 2026年第3期105-110,共6页
为研究基础饲粮中添加纳米硒对蛋鸡产蛋后期生产性能、蛋品质和抗氧化指标的影响,试验选取健康的57周龄京红1号蛋鸡150只,随机分为5组,每组3个重复,每个重复10只鸡,分别为C组(饲喂基础饲粮),NS-2组(饲喂基础饲粮+0.2 mg/kg的纳米硒),NS-... 为研究基础饲粮中添加纳米硒对蛋鸡产蛋后期生产性能、蛋品质和抗氧化指标的影响,试验选取健康的57周龄京红1号蛋鸡150只,随机分为5组,每组3个重复,每个重复10只鸡,分别为C组(饲喂基础饲粮),NS-2组(饲喂基础饲粮+0.2 mg/kg的纳米硒),NS-4组(饲喂基础饲粮+0.4 mg/kg的纳米硒),NS-6组(饲喂基础饲粮+0.6 mg/kg的纳米硒),SS组(饲喂基础饲粮+0.4 mg/kg的亚硒酸钠)。试验期共计7周,第1周为预饲期。结果显示:(1)与C组相比,NS-4组和SS组的产蛋率显著提高6.25%(P0.05)。(2)在蛋品质方面,硒处理组未显著提升鸡蛋品质,但NS-4组的蛋壳厚度、蛋壳强度、哈氏单位和蛋白高度均高于C组(P>0.05),分别提高1.06%、6.80%、36.63%和30.90%。与SS组相比,NS-4组的蛋壳厚度显著增加16.18%(P<0.05)。此外,NS-4组和NS-6组的哈氏单位分别提高63.17%和54.22%,蛋白高度显著提高69.45%和66.55%(P<0.05)。(3)在抗氧化指标方面,与C组相比,NS-2组和NS-4组血清谷胱甘肽过氧化物酶(GSH-Px)水平显著提高,其中NS-2组提高64.48%,NS-4组提高25.41%(P<0.05)。NS-4组肝脏GSH-Px水平显著提升34.92%(P<0.05)。此外,NS-4组血清超氧化物歧化酶(SOD)水平显著高于NS-6组,提高33.34%(P<0.05),肝脏SOD水平显著提高15.72%(P<0.05),而NS-2组和NS-6组的肝脏SOD水平则分别降低20.63%和17.88%(P<0.05)。综上所述,添加一定量的纳米硒可以改善产蛋后期蛋鸡的生产性能、蛋品质和抗氧化性能。 展开更多
关键词 纳米硒 蛋鸡 产蛋后期 生产性能 蛋品质 抗氧化指标
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葛仙米多糖纳米硒结构表征及其体外抗氧化、抑制癌细胞活性 认领 引用 被引量:1
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作者 陈野 寇德正 +2 位作者 商龙臣 李伟 程超 《食品工业科技》 EI CAS 北大核心 2026年第10期120-129,共10页
目的:探究葛仙米多糖(Polysaccharide of Nostoc sphaeroides Kützing,PNSK)对纳米硒(Nano-Selenium Particles,SeNP)的稳定修饰作用机制及修饰前后生理活性的变化。方法:以PNSK为原料,采用亚硒酸钠(Na2SeO3)-抗坏血酸(VC... 目的:探究葛仙米多糖(Polysaccharide of Nostoc sphaeroides Kützing,PNSK)对纳米硒(Nano-Selenium Particles,SeNP)的稳定修饰作用机制及修饰前后生理活性的变化。方法:以PNSK为原料,采用亚硒酸钠(Na2SeO3)-抗坏血酸(VC)氧化还原法制备葛仙米多糖纳米硒(Polysaccharide of Nostoc sphaeroides Kützing Nano-Selenium Particles,PNSK-SeNP),通过紫外可见光谱、高效液相色谱、X射线衍射、红外光谱、X射线光电子能谱等对PNSK及PNSK-SeNP的结构进行了表征,并对比分析了PNSK及PNSK-SeNP的抗氧化、抑制癌细胞活性。结果:PNSK-SeNP的硒含量为52.03±0.20 mg/g,粒径为166.83±4.51 nm,PNSK-SeNP制备前后的单糖摩尔比变化不显著。PNSK-SeNP的X射线光电子能谱图中具有Se 3d信号峰,硒元素以0价态存在,红外光谱和X射线光电子能谱图均表明PNSK-SeNP中葛仙米多糖的主要结构和官能团未发生变化,纳米硒与PNSK间未形成新的化学键。抗氧化测定发现,PNSK和PNSK-SeNP对羟自由基(·OH)的半抑制浓度(Half maximal inhibitory concentration,IC50)分别为4.45和0.55 mg/mL,对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS+)的IC50值分别为4.58和0.79 mg/mL,对A549肺癌细胞的IC50分别为0.87和0.06 mg/mL,同时PNSK-SeNP可以有效抑制A549肺癌细胞迁移,诱导其凋亡。相关性分析发现PNSK-SeNP的硒含量与其抗氧化、抑癌作用具有显著相关性。结论:PNSK可有效稳定纳米硒,且PNSK-SeNP的体外抗氧化、抑制癌细胞活性较PNSK均显著增加。 展开更多
关键词 葛仙米多糖 纳米硒 结构表征 抗氧化 抑制癌细胞
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Nano-selenium alleviates cadmium-induced cerebellar injury by activating metal regulatory transcription factor 1 mediated metal response 认领 引用 被引量:2
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作者 Shao-Shuai Bi Milton Talukder +4 位作者 Hai-Tao Jin Mei-Wei Lv Jing Ge Cong Zhang Jin-Long Li 《Animal Nutrition》 SCIE CAS CSCD 2022年第4期402-412,共11页
This study aims to investigate the role of metal regulatory transcription factor 1(MTF1)-mediated metal response in cadmium(Cd)-induced cerebellar injury,and to evaluate the antagonistic effects of nanoselenium(Nano-S... This study aims to investigate the role of metal regulatory transcription factor 1(MTF1)-mediated metal response in cadmium(Cd)-induced cerebellar injury,and to evaluate the antagonistic effects of nanoselenium(Nano-Se)against Cd toxicity.A total of 80 chicks(1 d old,male,Hy-Line Variety White)were randomly allocated to 4 treatment groups for 3 months:the control group(fed with a basic diet,n=20),the Nano-Se group(basic diet with 1 mg/kg nano-Se 1 mg/kg Nano-Se in basic diet,n=20),the NanoSe+Cd group(basic diet with 1 mg/kg Nano-Se and 140 mg/kg Cd Cl2,n=20)and the Cd group(basic diet with 140 mg/kg Cd Cl2,n=20).The results of the experiment showed that the Purkinje cells were significantly decreased with their degradation and indistinct nucleoli after Cd exposure.Moreover,exposure to Cd caused a significant accumulation of Cd and cupper.However,the contents of Se,iron,and zinc were decreased,thereby disturbing the metal homeostasis in the cerebellum.The Cd exposure also resulted in high levels of malondialdehyde(MDA)and down regulation of selenoprotein transcriptome.Furthermore,the expressions of MTF1,metallothionein 1(MT1),MT2,zinc transporter 3(ZNT3),ZNT5,ZNT10,zrt,irt-like protein 8(ZIP8),ZIP10,transferrin(TF),ferroportin 1(FPN1),ATPase copper transporting beta(ATP7B),and copper uptake protein 1(CTR1)were inhibited by Cd exposure.However,all these changes were significantly alleviated by the supplementation of Nano-Se.This study proved that Cd could disorder metal homeostasis and induce oxidative stress,whereas Nano-Se could relieve all these negative effects caused by Cd via activating the MTF1-mediated metal response in the cerebellum of chicken. 展开更多
关键词 Nano-selenium Cadmium Selenoprotein transcriptome MTF1-mediated metal response Cerebellar injury
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Effects of low and high levels of nano-selenium on intestinal microbiota of Chinese tongue sole(Cynoglossus semilaevis) 认领 引用 被引量:2
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作者 Lei Jia Chunxiu Chen +2 位作者 Na Zhao Xiaoxu He Bo Zhang 《Aquaculture and Fisheries》 2022年第6期623-631,共9页
Nano-selenium(Se)has attracted growing attention due to its high bioavailability,low toxicity and its possible applications in the prevention of oxidative damage in animal tissues.Recent research works have conveyed t... Nano-selenium(Se)has attracted growing attention due to its high bioavailability,low toxicity and its possible applications in the prevention of oxidative damage in animal tissues.Recent research works have conveyed that the use of nano-seleniumas food additive could have positive effects on the nutrition of aquatic animals.In this study,the intestinal bacterial communities associated with low and high concentrations of nano-selenium in the diet of Chinese tongue sole were characterized,through 16S rRNA V3–V4 sequencing.Samples were derived from fish fed with diet containing different nano-selenium level,0 mg/kg for the control group,1.6 mg/kg for low dose group,and 2.4 mg/kg for high dose group The intestinal microbial community structure showed a low overall diversity,among which the vibro genus occupied the most important position with more than 90%relative abundance in all three group.Significant differences in specie diversity occurred between the nanoselenium treated(both low dose[IL]and high dose[LH])groups and the control group(IC).Acinetobacter,Arthrobacter,and Streptophytes were the top three enriched genera after the treatment with nano-selenium compared to IC.Meantime,the weight gain rate of the LH group was 9%higher than control.The results revealed the effect of nano-selenium on microbial community structure of gut and growth performance of C.semilaevis,Future research exploring the interaction mechanism between micronutrients and the microbial communities would increase our understanding of the fish nutrition. 展开更多
关键词 Intestinal microbiota Nano-selenium Cynoglossus semilaevis 16S rRNA sequencing
茶多糖的提取及茶多糖-纳米硒体外抗氧化活性研究 认领 引用
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作者 王清 胡文静 +5 位作者 孙鹤 耿协强 王威 雷磊 朱静 涂剑秋 《粮食与油脂》 北大核心 2026年第7期130-137,共8页
以信阳毛尖茶梗为原料,采用超声波法提取茶多糖,在单因素试验基础上,以多糖得率为指标,采用响应面法进行工艺优化。经化学还原法制备茶多糖-纳米硒,分析其结构特征,并通过测定DPPH自由基、羟基自由基、ABTS自由基的清除能力评价其体外... 以信阳毛尖茶梗为原料,采用超声波法提取茶多糖,在单因素试验基础上,以多糖得率为指标,采用响应面法进行工艺优化。经化学还原法制备茶多糖-纳米硒,分析其结构特征,并通过测定DPPH自由基、羟基自由基、ABTS自由基的清除能力评价其体外抗氧化活性。结果表明:茶多糖的最佳提取工艺为超声时间25 min、超声温度60℃、超声功率259 W、料液比1∶24(g/mL),在此条件下多糖得率为9.64%。茶多糖可以很好地稳定纳米硒,且茶多糖和纳米硒之间存在相互作用,在1.0 mg/mL的质量浓度下,茶多糖-纳米硒对DPPH自由基、羟基自由基、ABTS自由基的清除率分别为82.17%、83.10%、80.67%。茶多糖-纳米硒的体外抗氧化能力显著优于茶多糖,有望将其开发为多功能补硒制剂和潜在的天然抗氧化剂。 展开更多
关键词 茶多糖 纳米硒 工艺优化 抗氧化活性
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叶面喷施纳米硒对小麦硒吸收、转运及分布的影响 认领 引用
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作者 刘家君 王德福 +2 位作者 秦娜娜 刘靖涛 刘小容 《麦类作物学报》 CAS CSCD 北大核心 2026年第7期943-952,共10页
富硒小麦是缺硒地区人群膳食补硒的有效途径。为探究硒在小麦各部位的吸收、转运和分布规律,以小麦品系川文2401为供试材料,叶面喷施清水(CK)和7.5 g·hm-2(C1)、15.0 g·hm-2(C2)、30.0 g·hm-2(C3)纳米... 富硒小麦是缺硒地区人群膳食补硒的有效途径。为探究硒在小麦各部位的吸收、转运和分布规律,以小麦品系川文2401为供试材料,叶面喷施清水(CK)和7.5 g·hm-2(C1)、15.0 g·hm-2(C2)、30.0 g·hm-2(C3)纳米硒,分析不同处理对小麦硒吸收、转运、籽粒硒形态、营养元素、生态风险、膳食硒摄入和产量及其构成因素的影响。结果表明,与CK相比,叶面喷施纳米硒后小麦各组织的硒含量显著提高,籽粒硒含量增加25.5~126.8倍;硒转运系数(TF)发生显著变化,其中旗叶与第1茎节、第2叶与第2茎节、第3叶与第3茎节及第4叶与第4茎节的TF分别降低85.5%~91.3%、64.7%~82.4%、75.0%~75.0%和50.0%~83.3%,穗轴与颖壳、穗轴与籽粒的TF分别增加209.4%~718.8%和111.4%~640.0%。CK的籽粒仅检测出硒代胱氨酸(SeCys)和硒代蛋氨酸(SeMet),SeCys占比较高;C1、C2和C3处理籽粒中新增1种硒形态,为甲基硒代半胱氨酸(MeSeCys),SeMet占比最高。与CK相比,C1、C2和C3处理籽粒的锰、锌、铜和蛋白质含量显著下降,降幅分别为12.7%~15.6%、4.1%~18.2%、6.8%~16.1%和6.9%~22.7%;铁含量显著增加,增幅46.4%~111.1%。C1处理的生态风险指数(Er)为51.6,最接近Er安全阈值(40.0),成人每日膳食硒摄入量(EDI)为297.2μg,处于推荐范围(60~400μg);CK的Er在安全阈值内,但EDI远低于推荐范围的最低值;C2、C3处理的Er和EDI均高于安全/推荐范围。与CK相比,C1、C2、C3处理的产量和千粒重显著提高,增幅分别为3.5%~8.5%和2.8%~4.4%;有效穗数和穗粒数无显著变化。综上,叶面喷施纳米硒可显著增加小麦籽粒硒含量和产量,并通过改善硒形态、占比及其他营养元素含量提高其品质,其中C1处理(7.5 g·hm-2)为纳米硒最优喷施浓度。 展开更多
关键词 小麦 纳米硒 硒转运 营养元素 产量
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芽孢杆菌合成纳米硒的物理化学表征及其应用于富硒番茄栽培 认领 引用
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作者 马镝 盛琪 +1 位作者 阚国仕 陈红漫 《湖北农业科学》 2026年第2期113-119,共7页
从富硒土壤中筛选一株纳米硒合成细菌MC-3,利用形态学观察和16S rDNA序列分析进行菌株鉴定,通过扫描电镜(Scanning electron microscope,SEM)、X射线能谱(Energy dispersive spectrometer,EDS)、X射线衍射(X-ray diffraction,XRD)及傅... 从富硒土壤中筛选一株纳米硒合成细菌MC-3,利用形态学观察和16S rDNA序列分析进行菌株鉴定,通过扫描电镜(Scanning electron microscope,SEM)、X射线能谱(Energy dispersive spectrometer,EDS)、X射线衍射(X-ray diffraction,XRD)及傅立叶红外光谱(Fourier transform infrared spectroscopy,FTIR)技术探究其所合成纳米硒(Selenium nanoparticles,SeNPs)的理化特性,并将其应用于富硒番茄栽培试验。结果表明,菌株MC-3为蜡样芽孢杆菌(Bacillus cereus),可还原亚硒酸钠生成球形的非晶态及晶态纳米硒颗粒,粒径范围在100~300 nm。SeNPs主要含C、O、Se等元素,同时含有-OH、C=O和C-O等官能团。将SeNPs作为叶面肥应用于富硒番茄栽培,当SeNPs浓度为70 mg/L时,番茄果实中硒含量可达0.090 mg/kg FW,同时,果实中可溶性蛋白质和维生素C含量分别较CK提高41.25%和35.00%。以上研究表明,适宜浓度的SeNPs作为叶面肥喷施可用于富硒番茄栽培,并可改善番茄果实品质。 展开更多
关键词 芽孢杆菌(Bacillus cereus) 纳米硒 物理化学表征 富硒番茄
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饲粮中添加不同剂量纳米硒对蛋鸡生产性能、免疫与抗氧化指标的影响 认领 引用 被引量:1
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作者 陈宇琪 姚先赐 +4 位作者 郑凯天 史永浩 李慧 王翀 杨彩梅 《饲料研究》 CAS 北大核心 2026年第5期28-33,共6页
试验旨在探究饲粮添加不同剂量纳米硒对蛋鸡生产性能、蛋品质、鸡蛋硒沉积量、免疫指标及抗氧化指标的影响。选取1440只同批次、健康状况良好的280日龄荣达凤达1号蛋鸡,随机分为4组,每组6个重复,每个重复60只。对照组、低剂量组、中剂... 试验旨在探究饲粮添加不同剂量纳米硒对蛋鸡生产性能、蛋品质、鸡蛋硒沉积量、免疫指标及抗氧化指标的影响。选取1440只同批次、健康状况良好的280日龄荣达凤达1号蛋鸡,随机分为4组,每组6个重复,每个重复60只。对照组、低剂量组、中剂量组及高剂量组分别在基础饲粮中添加0.35 mg/kg亚硒酸钠(T0组)、0.25 mg/kg纳米硒(T1组)、0.35 mg/kg纳米硒(T2组)、0.45 mg/kg纳米硒(T3组)。预试期3 d,正式试验期28 d。结果显示,T2组和T3组产蛋率显著高于T0组(P<0.05),料蛋比显著低于T0组(P<0.05)。T1组、T2组和T3组蛋白高度、哈氏单位显著高于T0组(P<0.05)。T2组和T3组的鸡蛋硒含量显著高于T0组(P<0.05)。T2组和T3组血清中免疫球蛋白A(IgA)含量及T2组免疫球蛋白G(IgG)含量显著高于T0组(P<0.05),T1组、T2组和T3组血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)含量显著低于T0组(P<0.05)。T2组血清中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性及总抗氧化能力(T-AOC)显著高于T0组(P<0.05),丙二醛(MDA)含量显著低于T0组(P<0.05)。研究表明,饲粮中添加适宜剂量的纳米硒可以有效改善蛋鸡生产性能和蛋品质,促进硒沉积,增强机体免疫与抗氧化能力,建议在蛋鸡饲粮中添加0.35 mg/kg纳米硒。 展开更多
关键词 蛋鸡 纳米硒 生产性能 蛋品质 硒沉积 免疫指标 抗氧化指标
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发状念珠藻胞外多糖纳米硒的制备及体外抗氧化和降血糖能力 认领 引用
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作者 段东洁 张炜 +3 位作者 张国栋 张浩 王晓辰 邢凤杰 《精细化工》 EI CAS CSCD 北大核心 2026年第7期1571-1579,共9页
以发状念珠藻(Nostoc flagelliforme)为原料,提取发状念珠藻胞外多糖(EPS),后经硝酸-亚硒酸钠法制备了发状念珠藻胞外多糖纳米硒(EPS-SeNPs)。采用单因素实验和响应面法优化了EPS-SeNPs的制备工艺。采用UV-Vis、FTIR、SEM和TGA对EPS和EP... 以发状念珠藻(Nostoc flagelliforme)为原料,提取发状念珠藻胞外多糖(EPS),后经硝酸-亚硒酸钠法制备了发状念珠藻胞外多糖纳米硒(EPS-SeNPs)。采用单因素实验和响应面法优化了EPS-SeNPs的制备工艺。采用UV-Vis、FTIR、SEM和TGA对EPS和EPS-SeNPs的理化性质和结构进行了表征,考察了EPS和EPS-SeNPs的抗氧化及降血糖能力。结果表明,在m(Na2SeO3)/m(EPS)=0.55、反应时间10.5 h、反应温度77℃的最佳制备条件下,EPS-SeNPs的硒含量为7.40 mg/g。硒元素的引入使EPS形貌改变,平均粒径(129.59 nm)变小、Zeta电位(–47.45 mV)的绝对值变大,从而提高了EPS在溶液体系中的稳定性,但其原始结构并未受到影响。EPS-SeNPs的UV-Vis吸收光谱在300 nm处出现硒元素的新峰。EPS-SeNPs对羟基自由基(·OH)、α超氧基自由基的清除能力和对α-葡萄糖苷酶、α-淀粉酶的活性抑制能力均与其质量浓度呈正相关,质量浓度为5.0 g/L的EPS-SeNPs溶液对·OH、·O2的清除率分别为50.98%和68.43%,对α-葡萄糖苷酶、α-淀粉酶的活性抑制率分别为72.42%和72.57%,显著高于EPS的自由基清除能力和酶活性抑制能力(P<0.05)。 展开更多
关键词 发状念珠藻 胞外多糖 纳米硒 抗氧化活性 降血糖活性 中药现代化技术
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生物纳米硒对刺参幼参生长性能、抗氧化能力及肠道菌群的影响 认领 引用
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作者 徐文昊 刘锟声 +5 位作者 王颖婕 邱惠龙 李梓萌 王连顺 黄家强 靳晓敏 《广东海洋大学学报》 CAS CSCD 北大核心 2026年第2期65-73,共9页
【目的】研究饲料中添加不同水平生物纳米硒对刺参(Apostichopus japonicus)幼参生长、硒沉积、抗氧化能力及肠道菌群的影响,为提升刺参养殖效率并培育高附加值富硒海参苗种提供科学依据。【方法】在基础饲料中分别添加0、0.5、1.0、2.0... 【目的】研究饲料中添加不同水平生物纳米硒对刺参(Apostichopus japonicus)幼参生长、硒沉积、抗氧化能力及肠道菌群的影响,为提升刺参养殖效率并培育高附加值富硒海参苗种提供科学依据。【方法】在基础饲料中分别添加0、0.5、1.0、2.0和4.0 mg/kg生物纳米硒(分别记为Se0、Se0.5、Se1.0、Se2.0和Se4.0),分别投喂初始体质量(11.33±0.21)g的幼参30 d,采样分析刺参生长性能、组织硒含量、抗氧化指标和肠道菌群。【结果】随生物纳米硒添加量增大,Se1.0组增质量率和特定生长率较对照组显著升高(P<0.05);与对照组相比,各实验组肥满度显著增加(P<0.05),脱水率显著降低(P<0.05);除Se0.5组,其他实验组饲料系数均显著降低(P<0.05)。体壁、肠道与体腔液中硒含量随生物纳米硒添加量增大均呈升高趋势。与对照组相比,体壁、肠道和体腔液中Se0.5和Se1.0组超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性显著升高(P<0.05);除体腔液中Se4.0组,其他实验组在各组织中丙二醛(MDA)含量均显著降低(P<0.05)。肠道菌群在门水平上,各组共有的优势菌群为假单胞菌门(Pseudomonadota);在属水平上,Sedimentitalea和Fluviibacterium是各组共有的优势菌群,其中Se1.0组有益菌属Fluviibacterium相对丰度最高。【结论】饲料中生物纳米硒的适宜添加质量分数为1.0 mg/kg,有利于提高刺参幼参生长性能、组织硒沉积,增强抗氧化能力,改善肠道菌群环境。 展开更多
关键词 刺参 生物纳米硒 生长性能 抗氧化能力 肠道菌群
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不同外源硒对豇豆的品质和硒代谢相关基因的影响 认领 引用
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作者 杜赛男 汪园 +4 位作者 肖春梅 周圣利 张晓芸 张宝晨 李丽 《长江蔬菜》 2026年第2期42-49,共8页
硒(Se)肥可促进植物生长发育并改善其营养品质。为探究硒肥对豇豆(Vigna unguiculata)品质的影响以及硒在植物体内的吸收、转运与转化机制,于大田条件下,以豇豆为试验材料,选取生物纳米硒和亚硒酸钠2种外源硒源,于豇豆初花期进行叶面喷... 硒(Se)肥可促进植物生长发育并改善其营养品质。为探究硒肥对豇豆(Vigna unguiculata)品质的影响以及硒在植物体内的吸收、转运与转化机制,于大田条件下,以豇豆为试验材料,选取生物纳米硒和亚硒酸钠2种外源硒源,于豇豆初花期进行叶面喷施处理。硒肥设置0、0.1、0.5、1.0、2.5、3.0 mmol/L 6个浓度梯度,测定豇豆豆荚中可溶性糖、可溶性蛋白、纤维素、维生素C等营养指标,以及硒含量与硒形态,并开展转录组测序分析。结果表明,适宜浓度的硒处理可提高上述营养物质含量;相同浓度下,亚硒酸钠对可溶性糖和可溶性蛋白含量的提升作用更为显著。转录组测序共获得91513条转录本序列,从中挖掘出35个硒相关基因(如甲硫氨酸γ-裂解酶等)及锌指蛋白基因,这些基因与硒形态变化密切相关。综合各项指标,在豇豆初花期叶面喷施1.0 mmol/L亚硒酸钠或2.5 mmol/L纳米硒,可有效改善豆荚营养品质,并通过调控硒代谢相关基因的表达揭示其潜在机理。试验结果为豇豆富硒品质优化提供了依据,也为进一步理解不同硒源在植物体内的响应机制提供了参考。 展开更多
关键词 纳米硒 亚硒酸钠 营养指标 转录组
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外源纳米硒和亚硒酸钠对魔芋生长的影响 认领 引用
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作者 杜慧娟 李奕斐 +3 位作者 王郑琪 刘晨钰 周郅昊 贺茂昌 《陕西农业科学》 2026年第4期63-67,共5页
以盆栽花魔芋为试验材料,分析不同浓度化学硒NaSeO3和生物纳米硒SeNPs对魔芋农艺性状、病虫害发生率及产量的影响。结果表明,化学硒和纳米硒对魔芋的株高和地径影响不显著。化学硒处理中,5 mg/kg、25 mg/kg和125 mg/kg NaSeO3处... 以盆栽花魔芋为试验材料,分析不同浓度化学硒NaSeO3和生物纳米硒SeNPs对魔芋农艺性状、病虫害发生率及产量的影响。结果表明,化学硒和纳米硒对魔芋的株高和地径影响不显著。化学硒处理中,5 mg/kg、25 mg/kg和125 mg/kg NaSeO3处理的魔芋软腐病发病率均较对照增加100%,但125mg/kg、200 mg/kg NaSeO3处理的魔芋虫伤率均较对照降低66.7%。5 mg/kg纳米硒处理的魔芋软腐病发病率和虫伤率较对照分别降低100%和66.7%。不同浓度纳米硒处理的魔芋鲜质量均高于相同浓度的化学硒处理。5 mg/kg纳米硒处理显著提高了魔芋球茎鲜质量和根茎个数,其分别较对照显著增加41.2%和46.2%。综上所述,纳米硒较化学硒对魔芋的防病促生效果更好,其中5~125 mg/kg纳米硒处理能够明显促进魔芋生长发育。 展开更多
关键词 花魔芋 农艺性状 纳米硒 化学硒 NaSeO3
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纳米硒在反刍动物体内的吸收与转化机制研究进展 认领 引用 被引量:1
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作者 谢建明 王锌和 《养殖与饲料》 2026年第1期22-28,共7页
与传统硒源相比,纳米硒(nano-selenium)在反刍动物体内具有更高的稳定性和生物利用度,纳米硒独特的吸收与转化机制对反刍动物的健康与生产性能具有积极的调控作用。本文以“高稳定性-高效吸收-精准转化”为机制框架,阐述纳米硒在反刍动... 与传统硒源相比,纳米硒(nano-selenium)在反刍动物体内具有更高的稳定性和生物利用度,纳米硒独特的吸收与转化机制对反刍动物的健康与生产性能具有积极的调控作用。本文以“高稳定性-高效吸收-精准转化”为机制框架,阐述纳米硒在反刍动物体内的代谢过程与生理效应。纳米硒在瘤胃中稳定性显著高于无机硒,主要通过小肠跨细胞(网格蛋白/窝蛋白介导内吞)和旁细胞(紧密连接调控)途径高效吸收,随后转化为硒化物并借助硒代半胱氨酸插入序列(SECIS)整合入GPx等硒蛋白,从而增强机体的抗氧化与免疫功能,并优化瘤胃发酵模式(如丙酸产量显著提高)。未来研究应着重解析纳米硒与反刍动物肠道微生物的互作机制,建立品种特异性剂量模型,并发展靶向递送技术,以进一步推动纳米硒在畜牧业中的高效安全应用。 展开更多
关键词 纳米硒 反刍动物 吸收机制 生物转化 生物利用度 硒蛋白
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海藻酸钠/壳聚糖/纳米硒复合膜的制备及其在草莓保鲜中的应用 认领 引用 被引量:8
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作者 王清 陈秋怡 +4 位作者 雷磊 孙鹤 赵艺飞 朱静 刘涛 《食品与发酵工业》 EI CAS CSCD 北大核心 2025年第17期317-325,共9页
以海藻酸钠、壳聚糖和纳米硒为成膜基材,通过溶液共混流涎成膜法制备一种新型复合膜,考察复合膜的厚度、机械性能、表面形貌和抑菌特性以及在草莓保鲜方面的应用效果。结果表明,当海藻酸钠与壳聚糖的质量比为1∶2时,海藻酸钠-壳聚糖-纳... 以海藻酸钠、壳聚糖和纳米硒为成膜基材,通过溶液共混流涎成膜法制备一种新型复合膜,考察复合膜的厚度、机械性能、表面形貌和抑菌特性以及在草莓保鲜方面的应用效果。结果表明,当海藻酸钠与壳聚糖的质量比为1∶2时,海藻酸钠-壳聚糖-纳米硒复合膜的工艺最佳,此时膜厚度为0.053 mm,水分含量为20.27%,抗拉强度为141 MPa,对大肠杆菌的抑菌圈直径为19.99 mm。用该复合膜对草莓进行覆膜保鲜,与未覆膜组对比,能够有效地减缓草莓的失水和硬度的下降,抑制维生素C含量的下降和微生物的生长。因此该复合膜作为一种环保的复合材料,在保鲜领域展现出良好的应用前景,为水果保鲜提供了新策略。 展开更多
关键词 海藻酸钠 壳聚糖 纳米硒 草莓 保鲜
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叶面喷施纳米硒对猕猴桃果实氨基酸含量及代谢组的影响 认领 引用 被引量:3
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作者 刘亚男 鲍丹丹 +4 位作者 张四普 牛佳佳 许志飞 杨永锋 鲁云风 《园艺学报》 CAS CSCD 北大核心 2025年第6期1575-1587,共13页
为探讨纳米硒对猕猴桃氨基酸营养成分的影响及机制,以‘徐香’猕猴桃为试材,采用0、10、15、20和25 mg·L-1纳米硒进行叶面喷施,测定氨基酸含量的变化,并对猕猴桃果实进行代谢组测序。结果发现:15 mg·L-1纳米硒喷施后苏... 为探讨纳米硒对猕猴桃氨基酸营养成分的影响及机制,以‘徐香’猕猴桃为试材,采用0、10、15、20和25 mg·L-1纳米硒进行叶面喷施,测定氨基酸含量的变化,并对猕猴桃果实进行代谢组测序。结果发现:15 mg·L-1纳米硒喷施后苏氨酸、缬氨酸、异亮氨酸、亮氨酸、苯丙氨酸等氨基酸含量显著升高,鲜味氨基酸占比提升。非靶向代谢组学共鉴定出12861种代谢物,通过KEGG、HMDB数据库,进一步分析了成熟猕猴桃果实中脂质和类脂质分子、苯丙烷和聚酮、有机氧化合物的生物合成和代谢,主要富集在氨基酸代谢、次生代谢产物、萜类化合物和聚酮代谢等代谢途径。纳米硒可能通过刺激次级代谢水平提高猕猴桃果实氨基酸含量。 展开更多
关键词 猕猴桃 纳米硒 氨基酸 营养评价 代谢组学
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