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A Stable,Reliable,Cost-Effective Technique Route for Ni Detection in Industrial Wastewater via a Microfluidic Paper-Based Platform 认领 引用
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作者 Xiuxia Li Qing’er Yao +11 位作者 Jiangyue Bai Zihang Wang Xiaolu Xiong Zifan Ning Songhe Liu Shiqi Xu Chunpan Zhang Yujiu Jiang Mingxu Chu Yanbo Yang Dong Jiang Junfeng Han 《Journal of Environmental & Earth Sciences》 CAS 2025年第4期280-290,共11页
Nickel(II)as one of the primary categories of heavy metals can lead to serious health problems if achieving the critical levels in the water.Thus,it is vital to propose a stable,reliable,and economical approach for de... Nickel(II)as one of the primary categories of heavy metals can lead to serious health problems if achieving the critical levels in the water.Thus,it is vital to propose a stable,reliable,and economical approach for detecting Ni ions.The microfluidic paper-based analytical devices(µPADs)are potential candidates for the detection of water quality parameters including pH,heavy ions,nitrite and so on.However,it suffers from a huge error caused by the environment and artificial mistakes.In this study,we proposed an improved technique route to increase the stability and reliability of microfluidic paper-based analytical devices.The main technique points include a stable light source,a matched camera,improved reliability of the devices,and effective calculated methods.Finally,we established 15 standard curves that could be used to detect nickel ions and obtained uniform colorimetric results with reliability and repeatability.With those improvements,the relative errors for the five types of real water samples from the Zhongshan industrial parks were reduced to 0.26%,14.78%,24.20%,50.29%and 3.53%,respectively.These results were conducive to exploring this technique for the detection of nickel ions in wastewater from the Zhongshan industrial parks.The results demonstrated that the above technique route is promising for the detection of other heavy metal ions in industrial effluent. 展开更多
关键词 Nickel Detection Industrial Wastewater Detection Microfluidic Paper-Based Chips Analytical Device
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Research Progress on Microfluidic Paper-based Analytical Devices for Point-of-care Testing 认领 引用 被引量：1
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作者 ZHANG Yuji XU Ruicheng SHAN Dan 《激光生物学报》 CAS 2025年第1期1-11,共11页
Point-of-care testing(POCT)refers to a category of diagnostic tests that are performed at or near to the site of the patients(also called bedside testing)and is capable of obtaining accurate results in a short time by... Point-of-care testing(POCT)refers to a category of diagnostic tests that are performed at or near to the site of the patients(also called bedside testing)and is capable of obtaining accurate results in a short time by using portable diagnostic devices,avoiding sending samples to the medical laboratories.It has been extensively explored for diagnosing and monitoring patients’diseases and health conditions with the assistance of development in biochemistry and microfluidics.Microfluidic paper-based analytical devices(μPADs)have gained dramatic popularity in POCT because of their simplicity,user-friendly,fast and accurate result reading and low cost.SeveralμPADs have been successfully commercialized and received excellent feedback during the past several decades.This review briefly discusses the main types ofμPADs,preparation methods and their detection principles,followed by a few representative examples.The future perspectives of the development inμPADs are also provided. 展开更多
关键词 point-of-care testing microfluidic paper-based analytical devices sensor personalized medical treatment portable diagnostic equipment
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Rapid fabrication of modular 3D paper-basedmicrofluidic chips using projection-based 3D printing 认领 引用 被引量：1
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作者 Mingjun Xie Zexin Fu +5 位作者 Chunfei Lu Sufan Wu Lei Pan Yong He Yi Sun Ji Wang 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2024年第5期611-623,共13页
Paper-based microchips have different advantages,such as better biocompatibility,simple production,and easy handling,making them promising candidates for clinical diagnosis and other fields.This study describes ametho... Paper-based microchips have different advantages,such as better biocompatibility,simple production,and easy handling,making them promising candidates for clinical diagnosis and other fields.This study describes amethod developed to fabricate modular three-dimensional(3D)paper-based microfluidic chips based on projection-based 3D printing(PBP)technology.A series of two-dimensional(2D)paper-based microfluidic modules was designed and fabricated.After evaluating the effect of exposure time on the accuracy of the flow channel,the resolution of this channel was experimentally analyzed.Furthermore,several 3D paper-based microfluidic chips were assembled based on the 2D ones using different methods,with good channel connectivity.Scaffold-based 2D and hydrogel-based 3D cell culture systems based on 3D paper-based microfluidic chips were verified to be feasible.Furthermore,by combining extrusion 3D bioprinting technology and the proposed 3D paper-based microfluidic chips,multiorgan microfluidic chips were established by directly printing 3D hydrogel structures on 3D paperbased microfluidic chips,confirming that the prepared modular 3D paper-based microfluidic chip is potentially applicable in various biomedical applications. 展开更多
关键词 Paper-based microfluidic chip Projection-based 3D printing(PBP) Modularization Cell culture
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Literature Review of the Use of Zinc and Zinc Compounds in Paper-Based Microfluidic Devices 认领 引用
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作者 Amer Charbaji Hojat Heidari-Bafroui +1 位作者 Constantine Anagnostopoulos Mohammad Faghri 《Journal of Minerals and Materials Characterization and Engineering》 2021年第3期257-270,共14页
Zinc and its compounds, alloys and composites play an important role in the modern day world and find application in almost every aspect that can improve the quality of our lives. This ranges from supplements and phar... Zinc and its compounds, alloys and composites play an important role in the modern day world and find application in almost every aspect that can improve the quality of our lives. This ranges from supplements and pharmaceuticals that are meant to improve our health and wellbeing to additives meant to guard or reduce corrosion in metals. However, over the past several years, a new area of technology has been garnering a great deal of attention and has made use of zinc and its compounds. This is with reference to paper-based microfluidic technology that offers several advantages and that keeps expanding in the amount of applications it covers. In this paper, a review is offered for the applications that have used zinc or zinc compounds in paper-based microfluidic devices. 展开更多
关键词 Zinc Zinc Oxide Zinc Compounds Paper-Based Devices Paper Microfluidics Low-Cost Platforms Point-of-Care Diagnostics
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Highly efficient labelling of extracellular vesicles for enhanced detection on a microfluidic platform 认领 引用
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作者 Shi Hu Rui Hao +2 位作者 Zitong Yu Huitao Zhang Hui Yang 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第2期441-445,共5页
Developing precise extracellular vesicles(EVs)labelling techniques with minimal disturbance is of great importance to the follow-up EVs detection and analysis.However,currently available methods such as using probes t... Developing precise extracellular vesicles(EVs)labelling techniques with minimal disturbance is of great importance to the follow-up EVs detection and analysis.However,currently available methods such as using probes to conjugate phospholipids or membrane proteins have certain limitations due to EV steric hindrance,dye aggregation,etc.Here,we present a microfluidic platform to enhance EVs’labelling efficiency and improve their detection.This platform provides excellent sample throughput and high-efficiency EV labelling at lower label concentrations with an optimized flowing rate.Flow cytometry analysis(FCM)and cellular uptake results show that EV labelling by utilizing this platform possesses the merits of a higher labelling efficiency with 64.1%relative improvement than conventional co-incubation method and a lower background noise.Moreover,this technique maintains EVs’size,morphology and biological activities.After the recipient cells uptake the EVs treated by the microfluidic platform,the spatial and temporal distribution of EVs in the cells are clearly observed.These results demonstrate that our method holds great potential in efficient labelling of EVs,which is essential to subsequent EV quantification and analysis. 展开更多
关键词 Microfluidic platform Extracellular vesicles Labelling PKH dye Flow cytometry
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Correction to“Biomimetic chloroplasts:Two-phase microfluidic platforms with selective permeability for artificial photosynthesis” 认领 引用
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作者 Xuming Zhang Xiaowen Huang Yaolei Wang 《Droplet》 EI CSCD 2026年第1期128-128,共1页
Wang G,Hong M,Yang C,et al.Biomimetic chloroplasts:Two-phase microfluidic platforms with selective permeability for artificial photosynthesis.Droplet.2025;4(4):e70019.http://gffzzd3cc09b8251d45dfsnufu9uo0xc0k69bk.ffgz.tsg.suse.edu.cn/10.1002/dro2.70019 The correspondi... Wang G,Hong M,Yang C,et al.Biomimetic chloroplasts:Two-phase microfluidic platforms with selective permeability for artificial photosynthesis.Droplet.2025;4(4):e70019.http://gffzzd3cc09b8251d45dfsnufu9uo0xc0k69bk.ffgz.tsg.suse.edu.cn/10.1002/dro2.70019 The corresponding author information in the published version was incomplete due to an oversight during submission.The correct corresponding authors are. 展开更多
关键词 selective permeability two phase microfluidic platforms microfluidic platforms artificial photosynthesisdroplet e https doiorg dro artificial photosynthesis biomimetic chloroplasts
Integrated 3D microstructured digital microfluidic platform for advanced 3D cell culture 认领 引用
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作者 Xiaojun Chen Xiaodong Lin +1 位作者 Haoran Li Yanwei Jia 《Microsystems & Nanoengineering》 SCIE EI CSCD 2025年第6期725-736,共12页
Three-dimensional(3D)cell culture systems better simulate the in vivo microenvironment by promoting intercellular interactions and functional expression,which are crucial for tissue engineering and regenerative medici... Three-dimensional(3D)cell culture systems better simulate the in vivo microenvironment by promoting intercellular interactions and functional expression,which are crucial for tissue engineering and regenerative medicine.However,conventional two-dimensional(2D)culture platforms fail to mimic the spatial complexity of in vivo tissues,often resulting in altered cellular behavior and limited physiological relevance.In this research,we introduce a 3D cell culture platform based on a digital microfluidic(DMF)system.This platform integrates DMF electrode actuation with 3Dprinted microstructure arrays,enabling precise capture and aggregation of cells within a defined 3D scaffold.While cells initially adhere in a 2D structure,they rapidly self-assemble into a 3D cell spheroid on the chip.The platform’s capabilities for droplet dispersion,fusion,and movement were validated using the 3D-printed DMF chip.The key parameters,such as applied voltage,microstructure height,and electrode spacing,were systematically investigated for their effects on droplet manipulation.Cell viability and proliferation assays in 24,48,and 72 hours confirmed that the 3D microstructured scaffolds exhibit excellent biocompatibility and provide a microenvironment favorable for in vivolike cell growth.Overall,this integrated DMF chip supports robust 3D cell growth and represents a versatile tool for applications in tissue engineering and regenerative medicine. 展开更多
关键词 simulate vivo microenvironment tissue engineering altered cellular behavior regenerative medicinehoweverconventional d cell culture platform digital microfluidic dmf system intercellular interactions functional expressionwhich
A programmable magnetic digital microfluidic platform integrated with electrochemical detection system 认领 引用 被引量：1
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作者 Yong Zhao Shuyue Jiang +3 位作者 Gaozhe Cai Lihua Wang Jianlong Zhao Shilun Feng 《Microsystems & Nanoengineering》 SCIE EI CSCD 2025年第3期495-506,共12页
Digital microfluidic(DMF)technology is widely used in bioanalysis and chemical reactions due to its accuracy and flexibility in manipulating droplets.However,most DMF systems usually rely on complex electrode fabricat... Digital microfluidic(DMF)technology is widely used in bioanalysis and chemical reactions due to its accuracy and flexibility in manipulating droplets.However,most DMF systems usually rely on complex electrode fabrication and high driving voltages.Sensor integration in DMF systems is also quite rare.In this study,a programmable magnetic digital microfluidic(PMDMF)platform integrated with electrochemical detection system was proposed.It enables non-contact,flexible droplet manipulation without complex processes and high voltages,meeting the requirements of automated electrochemical detection.The platform includes a magnetic control system,a microfluidic chip,and an electrochemical detection system.The magnetic control system consists of a microcoil array circuit board,a N52 permanent magnet,and an Arduino control module.N52 magnets generate localized magnetic fields to drive droplet movement,while the Arduino module enables programmable control for precise manipulation.The maximum average velocity of the droplet is about 3.9 cm/s.The microfluidic chip was fabricated using 3D printing and the superhydrophobic surface of chip was fabricated by spray coating.The electrochemical detection system consists of the MoS2@CeO2/PVA working electrode,Ag/AgCl reference electrode,and carbon counter electrode.To evaluate the practical value of the integrated platform,glucose in sweat was automatically and accurately detected.The proposed platform has a wide linear detection range(0.01–0.25 mM),a lower LOD(6.5μM),a superior sensitivity(7833.54μA·mM−1·cm−2),and excellent recovery rate(88.1-113.5%).It has an extensive potential for future application in the fields of medical diagnostics and point-of-care testing. 展开更多
关键词 digital microfluidic dmf technology electrochemical detection system dmf systems complex electrode fabrication programmable magnetic digital microfluidic platform programmable magnetic digital microfluidic pmdmf platform chemical reactions complex processes
Microfluidic platforms for monitoringcardiomyocyte electromechanical activity 认领 引用 被引量：2
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作者 Wei Wang Weiguang Su +5 位作者 Junlei Han Wei Song Xinyu Li Chonghai Xu Yu Sun Li Wang 《Microsystems & Nanoengineering》 SCIE EI CSCD 2025年第1期23-44,共22页
Cardiovascular diseases account for ~40% of global deaths annually. This situation has revealed the urgent need forthe investigation and development of corresponding drugs for pathogenesis due to the complexity of res... Cardiovascular diseases account for ~40% of global deaths annually. This situation has revealed the urgent need forthe investigation and development of corresponding drugs for pathogenesis due to the complexity of researchmethods and detection techniques. An in vitro cardiomyocyte model is commonly used for cardiac drug screeningand disease modeling since it can respond to microphysiological environmental variations through mechanoelectricfeedback. Microfluidic platforms are capable of accurate fluid control and integration with analysis and detectiontechniques. Therefore, various microfluidic platforms (i.e., heart-on-a-chip) have been applied for the reconstruction ofthe physiological environment and detection of signals from cardiomyocytes. They have demonstrated advantages inmimicking the cardiovascular structure and function in vitro and in monitoring electromechanical signals. This reviewpresents a summary of the methods and technologies used to monitor the contractility and electrophysiologicalsignals of cardiomyocytes within microfluidic platforms. Then, applications in common cardiac drug screening andcardiovascular disease modeling are presented, followed by design strategies for enhancing physiology studies. Finally,we discuss prospects in the tissue engineering and sensing techniques of microfluidic platforms. 展开更多
关键词 cardiac drug screeningand detection techniques microfluidic platforms investigation development corresponding drugs pathogenesis disease modeling accurate fluid control cardiovascular diseases vitro cardiomyocyte model
A paper-based microfluidic biosensor integrating zinc oxide nanowires for electrochemical glucose detection 认领 引用 被引量：7
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作者 Xiao Li Chen Zhao Xinyu Liu 《Microsystems & Nanoengineering》 SCIE 2015年第1期112-118,共7页
This paper reports an electrochemical microfluidic paper-based analytical device(EμPAD)for glucose detection,featuring a highly sensitive working electrode(WE)decorated with zinc oxide nanowires(ZnO NWs).In addition ... This paper reports an electrochemical microfluidic paper-based analytical device(EμPAD)for glucose detection,featuring a highly sensitive working electrode(WE)decorated with zinc oxide nanowires(ZnO NWs).In addition to the common features ofμPADs,such as their low costs,high portability/disposability,and ease of operation,the reported EμPAD has three further advantages.(i)It provides higher sensitivity and a lower limit of detection(LOD)than previously reportedμPADs because of the high surface-to-volume ratio and high enzyme-capturing efficiency of the ZnO NWs.(ii)It does not need any light-sensitive electron mediator(as is usually required in enzymatic glucose sensing),which leads to enhanced biosensing stability.(iii)The ZnO NWs are directly synthesized on the paper substrate via low-temperature hydrothermal growth,representing a simple,low-cost,consistent,and mass-producible process.To achieve superior analytical performance,the on-chip stored enzyme(glucose oxidase)dose and the assay incubation time are tuned.More importantly,the critical design parameters of the EμPAD,including the WE area and the ZnO-NW growth level,are adjusted to yield tunable ranges for the assay sensitivity and LOD.The highest sensitivity that we have achieved is 8.24μA·mM−1·cm−2,with a corresponding LOD of 59.5μM.By choosing the right combination of design parameters,we constructed EμPADs that cover the range of clinically relevant glucose concentrations(0−15 mM)and fully calibrated these devices using spiked phosphate-buffered saline and human serum.We believe that the reported approach for integrating ZnO NWs on EμPADs could be well utilized in many other designs of EμPADs and provides a facile and inexpensive paradigm for further enhancing the device performance. 展开更多
关键词 electrochemical detection enzymatic biosensor glucose detection paper-based microfluidics zinc oxide nanowire
复杂食品基质中污染物的纸基微流控检测进展 认领 引用
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作者 刘培华 张菊 +3 位作者 董莹 陈子凡 田君飞 张南峰 《包装与食品机械》 CAS 北大核心 2026年第4期12-25,共14页
针对食品污染物现场快速筛查中,基质复杂、检测流程分散和标准化验证不足等问题,系统梳理纸基微流控平台在食品污染物检测中的研究进展。围绕纸基材料与结构构型、信号转导与读出策略、典型污染物检测应用,以及实际部署瓶颈4个方面,对... 针对食品污染物现场快速筛查中,基质复杂、检测流程分散和标准化验证不足等问题,系统梳理纸基微流控平台在食品污染物检测中的研究进展。围绕纸基材料与结构构型、信号转导与读出策略、典型污染物检测应用,以及实际部署瓶颈4个方面,对近年来的相关研究进行归纳分析。现有研究已形成纤维素滤纸、硝化纤维素膜、玻璃纤维纸、办公纸、纳米纤维素纸和复合功能纸等多类基底,构建比色、荧光、化学发光、电化学、表面增强拉曼、光电化学及智能终端辅助读出等多种检测模式;部分代表性研究实现10 min内农药现场检测、4种重金属同步检测,实际样品回收率84.0%~104.1%。但在不同污染物类别、食品基质和信号读出方式下,纸基微流控平台的检出限、回收率、重复性和现场定量能力差异较大。纸基微流控平台具有低成本、便携和可集成等优势,但复杂食品基质干扰、器件批次一致性不足、前处理能力有限和标准化验证不足仍限制其应用。未来应加强模块化前处理、多模态信号验证、智能终端规范化读出和真实样品验证等。 展开更多
关键词 纸基微流控平台 食品污染物 信号机制 现场检测 数字化判读
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A fully automated rotary microfluidic platform for high-throughput multiplex detection of respiratory tract pathogens 认领 引用
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作者 Daoguangyao Zhang Anyi Li +7 位作者 Chuanlong Li Pei Ren Liming Zhang Bin Liu Shiyong Yu Xuefei Lv Wenzhe Si Yulin Deng 《Microsystems & Nanoengineering》 SCIE EI CSCD 2025年第5期489-498,共10页
Rapid,high-throughput,timely,multiplex diagnosis of respiratory-tract infections still relies on laboratory infrastructure,sequential assays,and trained personnel,thereby delaying targeted therapy and outbreak contain... Rapid,high-throughput,timely,multiplex diagnosis of respiratory-tract infections still relies on laboratory infrastructure,sequential assays,and trained personnel,thereby delaying targeted therapy and outbreak containment.In this study,a Fully Automated rotary microfluidic platform(FA-RMP)for high-throughput multiplex respiratory tract pathogens detection was presented.FA-RMP enables a true“sample-in,result-out”workflow through the integration of swab lysis,reagent partitioning,lyophilized reverse transcription loop-mediated isothermal amplification(RT-LAMP),and movingprobe fluorescence read-out,all encapsulated with a disposable microfluidic cartridge and paired with a 9 kg,fourchannel benchtop reader.The FA-RMP enables parallel processing of 16 independent reactions within 30 min,supporting simultaneous detection of up to 4 distinct clinical samples.Analytical validation using serially diluted Mycoplasma pneumoniae(MP)DNA established a limit of detection(LoD)of 50 copies μL-1 and a log-linear correlation between threshold time and template load(R2=0.9528).Testing with eight non-target respiratory pathogens yielded no amplification,confirming high analytical specificity.FA-RMP successfully detected the clinical samples with influenza A,influenza B,and MP,further demonstrating its robust multiplex detection capability.By integrating automated sample preparation,multiplex isothermal amplification and quantitative detection into a portable,high-throughput system,the platform delivers laboratory-grade performance at the point of care,serving as a scalable tool for routine respiratory pathogens screening and rapid epidemic response. 展开更多
关键词 swab lysisreagent partitioninglyophilized reverse transcripti targeted therapy rotary microfluidic platform fa rmp high throughput multiplex detection outbreak containmentin automated rotary microfluidic platform respiratory tract pathogens detection sample result workflow
A microfluidic platform for the co-culturing of microtissues with continuously recirculating suspension cells 认领 引用
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作者 Christian Lohasz Tamara Hafeli +11 位作者 Dzhansu Hasanova Lisa Holting Michal Rudnik Laure-Anne Ligeon Svenja Lützow Avni Mehta Bettina Kritzer Sandra Laternser Javad Nazarian Andreas Hierlemann Olivier Frey Mario M.Modena 《Microsystems & Nanoengineering》 SCIE EI CSCD 2025年第5期499-515,共17页
In vitro evaluation of novel therapeutic approaches often fails to reliably predict efficacy and toxicity,especially when recapitulating conditions involving recirculating cells.Current testing strategies are often ba... In vitro evaluation of novel therapeutic approaches often fails to reliably predict efficacy and toxicity,especially when recapitulating conditions involving recirculating cells.Current testing strategies are often based on static co-culturing of cells in suspension and 3D tissue models,where cell sedimentation on the target tissue can occur.The observed effects may then mostly be a consequence of sedimentation and of the corresponding forced cell-tissue interactions.The realization of continuous medium flow helps to better recapitulate physiological conditions and cell-tissue interactions.To tackle current limitations of perfused organ-on-chip approaches,we developed a microfluidic chip and operation concept,which prevents undesired sedimentation and accumulation of suspended cells during multiple days by relying on gravity-driven perfusion.Our platform,which we termed“human immune flow(hiFlow)chip”,enables to co-culture cells in suspension with up to 7 preformed microtissue models.Here,we present the design principle and operation of the platform,and we validate its performance by culturing cells and microtissues of a variety of different origins.Cells and tissues could be monitored on chip via high-resolution microscopy,while cell suspensions and microtissues could be easily retrieved for off-chip analysis.Our results demonstrate that primary immune cells and a range of different spheroid models of healthy and diseased tissues can be maintained for over 6 days on chip.As proof-of-concept cell-tissue interaction assay,we used an antibody treatment against diffuse midline glioma,a highly aggressive pediatric tumor.We are confident that our platform will help to increase the prediction power of in vitro preclinical testing of novel therapeutics that rely on the interaction of circulating cells with organ tissues. 展开更多
关键词 vitro evaluation microfluidic platform microtissues d tissue modelswhere co culturing target tissue continuously recirculating suspension cells continuous medium flow
Biomimetic chloroplasts:Two-phase microfluidic platforms with selective permeability for artificial photosynthesis 认领 引用
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作者 Guanxiong Wang Meiying Hong +8 位作者 Chonghui Yang Menghan Yang Gaozhen Zhao Haixia Liu Rui Shen Siping Wang Xuming Zhang Xiaowen Huang Yaolei Wang 《Droplet》 EI CSCD 2025年第4期51-62,共12页
The development of biomimetic chloroplasts offers significant potential in addressing global energy and environmental challenges.Traditional droplet-based models are limited by transmembrane transport inefficiencies,l... The development of biomimetic chloroplasts offers significant potential in addressing global energy and environmental challenges.Traditional droplet-based models are limited by transmembrane transport inefficiencies,leading to the accumulation of aqueous products that severely hinder reaction performance.In this work,we present a biomimetic chloroplast system that integrates light-dependent and lightindependent reactions,reaction compartments,and selectively permeable interfaces,fabricated using a biphasic microfluidic platform.The permeable interface facilitates continuous substrate–product exchange,mitigating product inhibition and side reactions,thus enhancing reaction efficiency.Furthermore,a quartz spiral tube was engineered to amplify Dean vortex effects,improving mass transfer.This system exhibited a nicotinamide adenine dinucleotide regeneration efficiency during lightdependent reactions that was 5.52 times higher than that of conventional slurry reactors.In the light-independent reaction,the energy conversion efficiency for the transformation ofα-ketoglutaric acid to L-glutamic acid reached 1.45 times that of natural photosynthesis.As the first comprehensive integration of photosynthetic processes within artificial chloroplasts,this work combines biological mechanisms with engineered components to establish a transformative platform for efficient energy conversion and directional biosynthesis.This breakthrough advances the field of photocatalysis and bioinspired technologies,with wide-reaching implications for sustainable energy and synthetic biology applications. 展开更多
关键词 transmembrane transport biphasic mi selective permeability aqueous products microfluidic platforms addressing global energy environmental challengestraditional biomimetic chloroplasts
基于微流控平台和深度学习的多时程细菌特征分析 认领 引用
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作者 廖阳 祖基翔 +3 位作者 俞帆 陈琪凯 滕新能 徐莹 《杭州电子科技大学学报（自然科学版）》 2026年第4期30-43,共14页
显微视野下多定点细菌生长图像的定时程分析对于测定最低抑菌浓度(Minimum Inhibitory Concentration,MIC)及研究抗生素对细菌生长的微观影响具有重要意义。为探究抗生素对细菌的抑制效果,研究人员构建了一种基于微观图像特征的微流控... 显微视野下多定点细菌生长图像的定时程分析对于测定最低抑菌浓度(Minimum Inhibitory Concentration,MIC)及研究抗生素对细菌生长的微观影响具有重要意义。为探究抗生素对细菌的抑制效果,研究人员构建了一种基于微观图像特征的微流控机械显微分析平台。针对数据采集过程中图像对比度较低及模型在检测细菌等小目标时性能不足的问题,本文提出了基于限制对比度的自适应直方图均衡化的图像增强方法,并设计了高效的跨阶段瓶颈结构、基于注意力的多尺度特征融合的改进模型。改进后的模型在细菌图像的mAP0.5指标上由74.9%提升至78.5%,在BCCD数据集上亦提升了1.5%。融合改进的YOLOv8(You Only Look Once Version 8)模型与分割一切模型(Segment Anything Model,SAM),构建了YOLO-SAM二阶段模型,用于提取细菌微观图像的面积特征。该模型进一步应用于分析不同浓度硫酸阿米卡星作用下细菌的面积大小分布变化。同时,比较了宏观光密度(Optical Density,OD)值和微观图像特征在多浓度抗生素条件下的分布梯度。通过对3550帧数据的分析,确定硫酸阿米卡星的最低抑菌浓度约为7.5μg/mL。不同浓度抗生素对细菌生长的动态影响可在约2 h内显现,显著快于传统OD值变化方法。该方法可实现对任意目标样本的不同时程生长情况的分析,具备准确、快速、可追踪及样本数据容量大的优势。 展开更多
关键词 微流控机械显微平台 YOLO-SAM二阶段模型 细菌生长定量分析 抗生素抑菌浓度评估
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Scalable Methods for Device Patterning as an Outstanding Challenge in Translating Paper-Based Microfluidics from the Academic Benchtop to the Point-of-Care 认领 引用
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作者 Jessica C.Brooks Charles R.Mace 《Journal of Analysis and Testing》 EI 2019年第1期50-60,共11页
Paper-based microfluidic devices offer unparalleled adaptability for the development of low-cost,point-of-care analytical tests.The potential for these devices to drastically improve access to healthcare around the gl... Paper-based microfluidic devices offer unparalleled adaptability for the development of low-cost,point-of-care analytical tests.The potential for these devices to drastically improve access to healthcare around the globe is obvious,but very few tests have found success in clinical environments.Here,we identify manufacturing-specifically,methods to pattern paper devices at large scales-as a major barrier to translating prototype paper-based devices from the academic benchtop to the field.We introduce current methods used to pattern papers and discuss their utility as means to prototype and manufacture paper-based devices. 展开更多
关键词 Paper-based microfluidics μPAD Point-of-care Diagnostics Manufacturing
Recent advances in high-throughput droplet-based microfluidic screening platforms for food analysis 认领 引用 被引量：1
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作者 Qianqian Zhang Shiqiang Yue +3 位作者 Dongyuan Zhang Fuping Lu Shuhong Mao Hui-Min Qin 《Food Bioscience》 SCIE 2024年第6期1192-1203,共12页
As a novel type of miniaturized instrumentation,high-throughput droplet-based microfluidics is playing an increasingly significant role in food safety inspection and quality control.The popularization of traditional i... As a novel type of miniaturized instrumentation,high-throughput droplet-based microfluidics is playing an increasingly significant role in food safety inspection and quality control.The popularization of traditional instrumental analysis is largely limited by the need for lengthy analysis time and costly instrumentation.The novel high-throughput droplet-based microfluidic screening platform(DMSP)has advantages of high screening rate,single-cell packaging,and less reagent consumption that solves the drawbacks of traditional instruments.In this review,we introduce the generation and manipulation of high-throughput DMSP,after which we summarize their recent applications in food analysis.These applications were demonstrated in the directed evolution of microorganisms for fermented food,improving the catalytic efficiency of enzymes for food processing,screening of nutraceutical ingredients,and detection of hazardous substances.We also provide a critical evaluation of the state of high-throughput DMSP and suggestions for future development directions. 展开更多
关键词 Droplet-based microfluidic screening platform High-throughput screening Single-cell packaging Food safety inspection Food quality control
Microfluidic platform serves as controllable fabrication of binary Mo/Ir nanodots/carbon hetero-material for efficient electrocatalytic nitrogen reduction 认领 引用 被引量：1
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作者 Hengyuan Liu Xingjiang Wu Jianhong Xu 《Particuology》 SCIE EI CAS CSCD 2023年第8期1-9,共9页
Electrocatalytic nitrogen reduction reaction(NRR)is regarded as a potential routine to achieve environment-friendly ammonia production,because of its abundant nitrogen resources,clean energy utilization and flexible o... Electrocatalytic nitrogen reduction reaction(NRR)is regarded as a potential routine to achieve environment-friendly ammonia production,because of its abundant nitrogen resources,clean energy utilization and flexible operation.However,it is hindered by low activity and selectivity,in which con-dition well-designed catalysts are urgently in need.In this work,a binary Mo/Ir nanodots/carbon(Mo/Ir/C)hetero-material is efficiently constructed via microfluidic strategy,of which the nanodots are ho-mogeneously distributed on the carbon skeleton and the average size is approximately 1 nm.Excellent performance for NRR is obtained in 1 mol L-1 KOH,of which the optimized ammonia yield and faradic efficiency are 7.27μg h-1 cm-2 and 2.31%respectively.Moreover,the optimized ammonia yield of 6.20μg h-1 cm-2 and faradic efficiency of 10.59%are also obtained in 0.005 mol L-1 H2SO4.This work achieves the continuous-flow synthesis and controllable adjustment of hetero-materials for favorable morphologies,which provides an innovative pathway for catalyst design and further promotes the development of ammonia production field. 展开更多
关键词 Binary Mo/Ir nanodots Microfluidic platform Controllable fabrication Flexible modification Electrocatalytic nitrogen reduction
Facile and Low-Cost Fabrication of a Thread/Paper-Based Wearable System for Simultaneous Detection of Lactate and pH in Human Sweat 认领 引用 被引量：8
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作者 Gang Xiao Jing He +6 位作者 Yan Qiao Feng Wang Qingyou Xia Xin Wang Ling Yu Zhisong Lu Chang-Ming Li 《Advanced Fiber Materials》 SCIE EI CAS 2020年第5期265-278,共14页
Wearable devices have received tremendous interests in human sweat analysis in the past few years.However,the widely used polymeric substrates and the layer-by-layer stacking structures greatly influence the cost-effi... Wearable devices have received tremendous interests in human sweat analysis in the past few years.However,the widely used polymeric substrates and the layer-by-layer stacking structures greatly influence the cost-efficiency,conformability and breathability of the devices,further hindering their practical applications.Herein,we report a facile and low-cost strategy for the fabrication of a skin-friendly thread/paper-based wearable system consisting of a sweat reservoir and a multi-sensing component for simultaneous in situ analysis of sweat pH and lactate.In the system,hydrophilic silk thread serves as the micro-channel to guide the liquid flow.Filter papers were functionalized to prepare colorimetric sensors for lactate and pH.The smartphone-based quantitative analysis shows that the sensors are sensitive and reliable.Although pH may interfere the lactate detection,the pH detected simultaneously could be employed to correct the measured data for the achievement of a precise lactate level.After being integrated with a hydrophobic arm guard,the system was successfully used for the on-body measurement of pH and lactate in the sweats secreted from the volunteers.This low-cost,easy-to-fabricate,light-weight and flexible thread/paper-based microfluidic sensing device may hold great potentials as a wearable system in human sweat analysis and point-of-care diagnostics. 展开更多
关键词 Wearable sensors Thread/paper-based microfluidics Sweat analysis Point-of-care diagnostics Multi-sensing system
Downregulation of EphB2 by RNA interference attenuates glial/fibrotic scar formation and promotes axon growth 认领 引用 被引量：4
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作者 Jian Wu Zhen-Yu Zhu +3 位作者 Zhi-Wei Fan Ying Chen Ri-Yun Yang Yi Li 《Neural Regeneration Research》 SCIE CAS CSCD 2022年第2期362-369,共8页
The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent intera... The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent interaction is a trigger for glial/fibrotic scar formation. In the present study, a new in vitro model was produced by coculture of fibroblasts and astrocytes wounded by scratching to mimic glial/fibrotic scar-like structures using an improved slide system. After treatment with RNAi to downregulate Eph B2, changes in glial/fibrotic scar formation and the growth of VSC4.1 motoneuron axons were examined. Following RNAi treatment, fibroblasts and astrocytes dispersed without forming a glial/fibrotic scar-like structure. Furthermore, the expression levels of neurocan, NG2 and collagen I in the coculture were reduced, and the growth of VSC4.1 motoneuron axons was enhanced. These findings suggest that suppression of Eph B2 expression by RNAi attenuates the formation of a glial/fibrotic scar and promotes axon growth. This study was approved by the Laboratory Animal Ethics Committee of Jiangsu Province, China(approval No. 2019-0506-002) on May 6, 2019. 展开更多
关键词 astrocyte EphB2 fibroblast glial/fibrotic scar microfluidic platform RNAi spinal cord injury VSC4.1 motoneuron
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