An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of a...An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of adverse geological conditions in deep-buried tunnel construction.The installation techniques for microseismic sensors were optimized by mounting sensors at bolt ends which significantly improves signal-to-noise ratio(SNR)and anti-interference capability compared to conventional borehole placement.Subsequently,a 3D wave velocity evolution model that incorporates construction-induced disturbances was established,enabling the first visualization of spatiotemporal variations in surrounding rock wave velocity.It finds significant wave velocity reduction near the tunnel face,with roof and floor damage zones extending 40–50 m;wave velocities approaching undisturbed levels at 15 m ahead of the working face and on the laterally undisturbed side;pronounced spatial asymmetry in wave velocity distribution—values on the left side exceed those on the right,with a clear stress concentration or transition zone located 10–15 m;and systematically lower velocities behind the face than in front,indicating asymmetric rock damage development.These results provide essential theoretical support and practical guidance for optimizing dynamic construction strategies,enabling real-time adjustment of support parameters,and establishing safety early warning systems in deep-buried tunnel engineering.展开更多
Fire emergencies have long posed a serious threat to people's lives,real estate assets,and environmental sustainability in civilized societies,especially when combustible events are detected at late stages of deve...Fire emergencies have long posed a serious threat to people's lives,real estate assets,and environmental sustainability in civilized societies,especially when combustible events are detected at late stages of development.Recent advancements in computer vision-based fire detection have enabled automated real-time monitoring;however,most solutions either detect the existence of fire/smoke or employ binary decision-making,which limits visual monitoring systems from being risk-aware.This work introduces a severity-aware fire/smoke detection model that supports intelligent monitoring systems in detecting visual hazards.The goal is to identify varying levels of fire intensity and smoke density and to detect humans in real time.We design a system capable of monitoring environments using components such as sensing devices,network communication buses,cloud data centers,and computer visionbased detectors.The fire/smoke detection model comprises modern deep learning-based object detectors,with the YOLOvl2 model serving as the detection backbone.Moreover,our work proposes a two-stage training method that first learns coarse representations of fire,smoke,and humans,and then adapts the detector for fine-grained,severityaware classification,thereby enhancing severity discrimination and reducing inter-class confusion.We gathered our dataset to comprise approximately 65oo annotated images,split between coarse-grained and severity-aware detection models.The dataset consists of seven classes indicating human presence,three classes indicating varying levels of fire intensity,and three classes indicating varying levels of smoke density.We conducted experiments comparing three baseline object detection architectures(i.e.,YOLOvl2s,RT-DETR-L,and SSDLite320-MobileNetV3)using identical trainingesting configurations.YOLOvl2 has outperformed other baseline object detection architectures,achieving 0.929 mAP@50,0.884 precision,0.876 recall,0.880 F1-score,and 2.48 ms per-image latency,providing the best balance between detection performance and real-time processing capability.Our results indicate that severity-aware detection can improve the early-stage detection of intelligent monitoring systems.展开更多
Tunnels are essential components of contemporary infrastructure,yet guaranteeing their safety,longevity,and efficiency remains a persistent challenge.Recent breakthroughs in artificial intelligence(AI)and digital twin...Tunnels are essential components of contemporary infrastructure,yet guaranteeing their safety,longevity,and efficiency remains a persistent challenge.Recent breakthroughs in artificial intelligence(AI)and digital twin(DT)technology provide innovative solutions for the real-time monitoring of tunnel systems,suggesting proactive maintenance tactics and improved safety protocols.This review paper offers a comprehensive examination of the application of AI and DT methodologies in tunnel surveillance.We explore the core concepts of AI and DT and their applicability to structural monitoring,encompassing machine learning,computer vision,and sensor integration.Through the utilization of these AI-powered technologies,engineers are equipped with unparalleled insights into the state and behavior of tunnels,facilitating the early identification of irregularities and the optimization of maintenance timelines.We discuss the array of AI techniques utilized for the immediate monitoring of tunnel systems,emphasizing their foundations,benefits,and practical uses.Numerous studies have showcased the effectiveness and adaptability of AI-based monitoring systems in various tunnel settings.Moreover,we address the hurdles and constraints inherent in AI and DT methodologies and suggest strategies for overcoming them,such as data augmentation,interpretable AI,edge computing,and continuous monitoring.Ultimately,the incorporation of AI and DT technologies into tunnel surveillance signifies a paradigm shift,offering substantial advantages over conventional techniques.By adopting AI-driven monitoring systems,tunnel operators can augment safety,prolong the lifespan of infrastructure,and decrease operational expenses,molding the future of subterranean infrastructure management.展开更多
With the accelerated pace of urbanization, structural safety of buildings has garnered significant attention. Addressing the limitations of traditional monitoring methods—such as slow response times and delayed infor...With the accelerated pace of urbanization, structural safety of buildings has garnered significant attention. Addressing the limitations of traditional monitoring methods—such as slow response times and delayed information transmission—researchers have conducted in-depth investigations into real-time building structure monitoring and developed an integrated monitoring system utilizing multiple sensor technologies. The study first examines various risks associated with prolonged building use, emphasizing the critical role of real-time monitoring systems in early defect detection and maintenance management. By employing sensor network technology, the system successfully collects and continuously monitors real-time data on structural vibrations, temperature fluctuations, and humidity levels. Through systematic integration and optimized data acquisition modules, all monitoring data are promptly transmitted to designated locations for immediate visualization. Practical testing demonstrated the system's high accuracy and reliability in rapid response and early fault identification, along with robust adaptability and scalability. These findings not only enhance the intelligence of building structural safety monitoring technologies but also provide robust theoretical foundations and practical guidelines for proactive maintenance and risk mitigation, thereby extending building service life while significantly reducing maintenance costs. This comprehensive planning scheme effectively safeguards building safety while significantly enhancing the operational efficiency of monitoring systems. It holds substantial practical value for widespread application and plays a crucial theoretical and practical role in accelerating the digital transformation of building management and further improving the overall security standards of urban infrastructure.展开更多
Objectives To develop and validate a machine learning(ML)model for real-time monitoring of in-hospital mortality(IHM)risk and identification of major risk factors in heart failure(HF)patients admitted to intensive car...Objectives To develop and validate a machine learning(ML)model for real-time monitoring of in-hospital mortality(IHM)risk and identification of major risk factors in heart failure(HF)patients admitted to intensive care units(ICUs).Methods Data from ICU HF patients were extracted from the multicenter eICU-Collaborative Research Database(eICU-CRD.External validation used MIMIC-IV and a real-world Chinese dataset(CHN-dataset).Daily measurements from MIMIC-IV patients staying≥3 days formed a Daily Measurement(DM)dataset.After rigorous preprocessing and feature selection,five ML algorithms were trained and optimized using eICU-CRD data.Model performance was evaluated using AUC,sensitivity,specificity,and balanced accuracy.The optimal model was benchmarked against APACHE and SOFA scores.SHapley Additive ex-Planations(SHAP)interpreted feature contributions.A Windows application was developed for clinical deployment.Results XGBoost emerged as the optimal model(Final-ML model),which incorporated only 17 routinely collected clinical variables:age,non-invasive systolic blood pressure(NI-SBP),heart rate,respiratory rate,Glasgow Coma Scale eye opening score,white blood cell count(WBC),creatinine,bicarbonate,red cell distribution width(RDW),platelet count,glucose,calcium,mean corpuscular hemoglobin concentration(MCHC),sodium,mean corpuscular volume,red blood cell count,and potassium.It achieved high AUCs:0.876(95%CI:0.836-0.915;eICU-CRD test data),0.932(95%CI:0.921-0.942;MIMIC-IV),and 0.879(95%CI:0.846-0.912;CHN-dataset).It significantly outperformed APACHE(AUC=0.740,95%CI:0.720-0.761)and SOFA(AUC=0.717,95%CI:0.694-0.740)scores.The model demonstrated strong generalizability across ethnicities,ward types,and genders within MIMIC-IV.Using daily data(DM dataset),predicted IHM risk accurately tracked patient trajectories:risk decreased progressively for survivors and increased for non-survivors throughout the ICU stay.SHAP analysis identified key predictors:NI-SBP,age,heart rate,WBC,glucose,and notably,RDW and MCHC.Time-dependent Cox regression confirmed RDW increase(HR=3.783,95%CI:2.237-6.398)and MCHC decrease(HR=0.173,95%CI:0.040-0.741)as significant independent risk factors for IHM.Conclusions The developed XGBoost model provides a reliable,generalizable tool for real-time IHM risk quantification and monitoring in ICU HF patients,using only 17 routinely collected clinical variables.It surpasses traditional scoring systems and enables dynamic risk assessment throughout the ICU stay.By identifying patient-specific major risk factors via SHAP values,the model facilitates timely,personalized treatment adjustments.展开更多
Electroacoustic Tomography(EAT)is an imaging technique that detects ultrasound waves induced by electrical pulses,offering a solution for real-time electroporation monitoring.This study presents EAT system using a dua...Electroacoustic Tomography(EAT)is an imaging technique that detects ultrasound waves induced by electrical pulses,offering a solution for real-time electroporation monitoring.This study presents EAT system using a dual-frequency ultrasound array.The broadband nature of electroacoustic signals requires ultrasound detector to cover both the high-frequency range(around 6MHz)signals generated by small targets and the low-frequency range(around 1MHz)signals generated by large targets.In our EAT system,we use the 6 MHz array to detect high-frequency signals from the electrodes,and the 1 MHz array for the electrical field.To test this,we conducted simulations using COMSOL Multiphysics® and MATLAB's k-Wave toolbox,followed by experiments using a custom-built setup with a dual-frequency transducer and real-time data acquisition.The results demonstrated that the dual-frequency EAT system could accurately and simultaneously monitor the electroporation process,effectively showing both the treatment area and electrode placement with the application of 1 kV electric pulses with 100 ns duration.The axial resolution of the 6MHz array for EAT was 0.45 mm,significantly better than the 2mm resolution achieved with the 1MHz array.These findings validate the potential of dual-frequency EAT as a superior method for real-time electroporation monitoring.展开更多
Acetylcholinesterase(AChE)plays a crucial role in the activities of the nervous system,and its abnormal function can lead to the occurrence and development of neurodegenerative diseases.Hence,an effective method for r...Acetylcholinesterase(AChE)plays a crucial role in the activities of the nervous system,and its abnormal function can lead to the occurrence and development of neurodegenerative diseases.Hence,an effective method for real-time monitoring of AChE activity is essential.Very recently,several fluorescence sensors have been developed for the detection of AChE activity,but they are usually imaging in the visible region,relatively small Stokes shifts,or long response times,limiting their application for real-time monitoring in vivo.Inspired by that,a near-infrared(NIR)off-on probe((E)-4-(2-(4-(dicyanomethylene)-4H-chromen-2-yl)vinyl)phenyl dimethylcarbamate,DCM-N)for AChE monitoring with high selectivity and sensitivity is developed.In the probe DCM-N,a bright near-infrared fluorescence emission at 700 nm can be triggered by AChE through the cleavage of amino ester bond in DCM-N,and the resulting fluorescence exhibits a good linear relationship with AChE activity in the range of 0.2–16 U/mL,with a detection limit as low as 0.06 U/mL.For real plasma sample detection,DCM-N demonstrates advantages of accurate detection and fast response compared to the traditional Ellman assay for AChE detection.Moreover,DCM-N can be used for imaging of AChE activity in live cells and tracking of AChE activity in zebrafish models,which is of great significance for medical and physiological research related to AChE.DCM-N possesses several notable features such as light-up NIR emission,fast response,large spectral shifts and strong photostability under physiological conditions.These features enable it to monitor AChE activity both in vivo and in vitro,providing a suitable tool for real-time monitoring and in vivo visualization of AChE activity.展开更多
Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter...Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter.Therefore,the need to monitor disc cutter wear in real-time has emerged as a technical challenge for TBMs.In this study,real-time disc cutter wear monitoring is developed based on sound and vibration sensors.For this purpose,the microphone and accelerometer were used to record the sound and vibration signals of cutting three different types of rocks with varying abrasions on a laboratory scale.The relationship between disc cutter wear and the sound and vibration signal was determined by comparing the measurements of disc cutter wear with the signal plots for each sample.The features extracted from the signals showed that the sound and vibration signals are impacted by the progression of disc wear during the rock-cutting process.The signal features obtained from the rock-cutting operation were utilized to verify the machine learning techniques.The results showed that the multilayer perceptron(MLP),random subspace-based decision tree(RS-DT),DT,and random forest(RF)methods could predict the wear level of the disc cutter with an accuracy of 0.89,0.951,0.951,and 0.927,respectively.Based on the accuracy of the models and the confusion matrix,it was found that the RS-DT model has the best estimate for predicting the level of disc wear.This research has developed a method that can potentially determine when to replace a tool and assess disc wear in real-time.展开更多
Combining the background of modern construction engineering site safety management,this article analyzes the real-time monitoring and alarm strategies for site construction safety under the integration of BIM and AI.T...Combining the background of modern construction engineering site safety management,this article analyzes the real-time monitoring and alarm strategies for site construction safety under the integration of BIM and AI.This includes the analysis of BIM and AI technologies and their integration advantages,real-time monitoring and alarm strategies for construction site safety based on BIM and AI integration,as well as the development direction of BIM and AI integration in real-time monitoring and alarm for construction site safety.It is hoped that through this analysis,a scientific reference can be provided for the digital and intelligent management of construction site safety,promoting the digital and intelligent development of its safety management work.展开更多
The Internet of Things technology provides a comprehensive solution for the real-time monitoring of cold chain logistics by integrating sensors,wireless communication,cloud computing,and big data analysis.Based on thi...The Internet of Things technology provides a comprehensive solution for the real-time monitoring of cold chain logistics by integrating sensors,wireless communication,cloud computing,and big data analysis.Based on this,this paper deeply explores the overview and characteristics of the Internet of Things technology,the feasibility analysis of the Internet of Things technology in the cold chain logistics monitoring,the application analysis of the Internet of Things technology in the cold chain logistics real-time monitoring to better improve the management level and operational efficiency of the cold chain logistics,to provide consumers with safer and fresh products.展开更多
Bioreactors are used to dynamically condition engineered tissues to achieve the required degree of maturation before in vivo implantation.Integrating sensors and imaging capabilities into bioreactors can help us under...Bioreactors are used to dynamically condition engineered tissues to achieve the required degree of maturation before in vivo implantation.Integrating sensors and imaging capabilities into bioreactors can help us understand how the culture environment influences tissue maturation and growth.Additionally,this enables the monitoring of tissue constructs and provides critical information for quality control.This study aimed to develop a standardized,self-contained,uniaxial bioreactor module for the clinical manufacturing of tissue constructs;this system would benefit from unidirectional mechanical or electrical stimulation,or both.We achieved this goal by integrating stimulation and sensing components that provide an optimal culture environment and monitoring capabilities to improve tissue manufacturing.The uniaxial bioreactor module included integrated,user-friendly mechanical and electrical stimulations with force measurement to enhance the preconditioning of the engineered tissues.Also,a sensor loop and media exchange system were integrated to monitor the culture environment and cellular metabolites over time,and the camera system above the tissue construct enabled the macroscopic visualization of tissue maturation.Furthermore,the onboard media exchange system was programmed into the module to maintain aseptic culture conditions in the long term.Subsequently,using native skeletal muscle tissue and tissue-engineered skeletal muscle constructs,the performance of the uniaxial bioreactor module was validated for its application in preconditioning and enhancing tissue maturation.展开更多
Time, cost, and quality are three key control factors in rockfill dam construction, and the tradeoff among them is important. Research has focused on the construction time-cost-quality tradeoff for the planning or des...Time, cost, and quality are three key control factors in rockfill dam construction, and the tradeoff among them is important. Research has focused on the construction time-cost-quality tradeoff for the planning or design phase, built on static empirical data. However, due to its intrinsic uncertainties, rockfill dam construction is a dynamic process which requires the tradeoffto adjust dynamically to changes in construction conditions. In this study, a dynamic time-cost-quality tradeoff (DTCQT) method is proposed to balance time, cost, and quality at any stage of the construction process. A time-cost-quality tradeoff model is established that considers time cost and quality cost. Time, cost, and quality are dynamically estimated based on real-time monitoring. The analytic hierarchy process (AHP) method is applied to quantify the decision preferences among time, cost, and quality as objective weights. In addition, an improved non-dominated sorting genetic algorithm (NSGA-II) coupled with the technique for order preference by similarity to ideal solution (TOPSIS) method is used to search for the optimal compromise solution. A case study project is analyzed to demonstrate the applicability of the method, and the efficiency of the proposed optimization method is compared with that of the linear weighted sum (LWS) and NSGA-II.展开更多
[Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been propo...[Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been proposed for monitoring cow ruminant behavior,including video surveillance,sound recognition,and sensor monitoring methods.How‐ever,the application of edge device gives rise to the issue of inadequate real-time performance.To reduce the volume of data transmission and cloud computing workload while achieving real-time monitoring of dairy cow rumination behavior,a real-time monitoring method was proposed for cow ruminant behavior based on edge computing.[Methods]Autono‐mously designed edge devices were utilized to collect and process six-axis acceleration signals from cows in real-time.Based on these six-axis data,two distinct strategies,federated edge intelligence and split edge intelligence,were investigat‐ed for the real-time recognition of cow ruminant behavior.Focused on the real-time recognition method for cow ruminant behavior leveraging federated edge intelligence,the CA-MobileNet v3 network was proposed by enhancing the MobileNet v3 network with a collaborative attention mechanism.Additionally,a federated edge intelligence model was designed uti‐lizing the CA-MobileNet v3 network and the FedAvg federated aggregation algorithm.In the study on split edge intelli‐gence,a split edge intelligence model named MobileNet-LSTM was designed by integrating the MobileNet v3 network with a fusion collaborative attention mechanism and the Bi-LSTM network.[Results and Discussions]Through compara‐tive experiments with MobileNet v3 and MobileNet-LSTM,the federated edge intelligence model based on CA-Mo‐bileNet v3 achieved an average Precision rate,Recall rate,F1-Score,Specificity,and Accuracy of 97.1%,97.9%,97.5%,98.3%,and 98.2%,respectively,yielding the best recognition performance.[Conclusions]It is provided a real-time and effective method for monitoring cow ruminant behavior,and the proposed federated edge intelligence model can be ap‐plied in practical settings.展开更多
This review explores glucose monitoring and management strategies,emphasizing the need for reliable and userfriendly wearable sensors that are the next generation of sensors for continuous glucose detection.In additio...This review explores glucose monitoring and management strategies,emphasizing the need for reliable and userfriendly wearable sensors that are the next generation of sensors for continuous glucose detection.In addition,examines key strategies for designing glucose sensors that are multi-functional,reliable,and cost-effective in a variety of contexts.The unique features of effective diabetes management technology are highlighted,with a focus on using nano/biosensor devices that can quickly and accurately detect glucose levels in the blood,improving patient treatment and control of potential diabetes-related infections.The potential of next-generation wearable and touch-sensitive nano biomedical sensor engineering designs for providing full control in assessing implantable,continuous glucose monitoring is also explored.The challenges of standardizing drug or insulin delivery doses,low-cost,real-time detection of increased blood sugar levels in diabetics,and early digital health awareness controls for the adverse effects of injectable medication are identified as unmet needs.Also,the market for biosensors is expected to expand significantly due to the rising need for portable diagnostic equipment and an ever-increasing diabetic population.The paper concludes by emphasizing the need for further research and development of glucose biosensors to meet the stringent requirements for sensitivity and specificity imposed by clinical diagnostics while being cost-effective,stable,and durable.展开更多
The theory and method of system integration for the real-time monitoring of core rock-fill dam filling con- struction quality are studied in this paper. First, the importance analysis of system integration factors is ...The theory and method of system integration for the real-time monitoring of core rock-fill dam filling con- struction quality are studied in this paper. First, the importance analysis of system integration factors is carried out with the analytic hierarchy process. Then, according to the analysis result of integration factors, the conceptual model of system integration is built based on function integration, index integration, technology integration and information integration, the index structure of core rock-fill dam filling construction quality control is constructed and the method of function integration and technology integration is studied. The mathematical model of process monitoring is built according to monitoring objective, process and indexes. Research results have been applied in Nuozhadu core rock-fill dam construction management, realizing system integration through building appropriate monitoring work flow and comprehensive information platform of digital dam.展开更多
A new system called alternate data acquisition and real-time monitoring system has been developed for long-time discharge in tokamak operation. It can support continuous on-line data acquisition at a high sampling rat...A new system called alternate data acquisition and real-time monitoring system has been developed for long-time discharge in tokamak operation. It can support continuous on-line data acquisition at a high sampling rate and a graphic display of the plasma parameters during the discharge. Thus operators can monitor and control the plasma state in real time. An application of this system has been demonstrated on the HT-7 tokamak.展开更多
As global climate change intensifies,the power industry-a major source of carbon emissions-plays a pivotal role in achieving carbon peaking and neutrality goals through its low-carbon transition.Traditional power pla...As global climate change intensifies,the power industry-a major source of carbon emissions-plays a pivotal role in achieving carbon peaking and neutrality goals through its low-carbon transition.Traditional power plants’carbon management systems can no longer meet the demands of high-precision,real-time monitoring.Smart power plants now offer innovative solutions for carbon emission tracking and intelligent analysis by integrating IoT,big data,and AI technologies.Current research predominantly focuses on optimizing individual processes,lacking systematic exploration of comprehensive dynamic monitoring and intelligent decision-making across the entire workflow.To address this gap,we propose a smart carbon emission monitoring and analysis platform for power plants that integrates IoT sensing,multimodal data analytics,and AI-driven decision-making.The platform establishes a multi-source sensor network to collect emissions data throughout the fuel combustion,auxiliary equipment operation,and waste treatment processes.Combining carbon emission factor analysis with machine learning models enables real-time emission calculations and utilizes long short-term memory networks to predict future emission trends.展开更多
Monitoring the thickness changes of channel siltation is paramount in safeguarding navigation and guiding dredging, This paper presents a novel method for realizing the field monitoring of channel siltation in real ti...Monitoring the thickness changes of channel siltation is paramount in safeguarding navigation and guiding dredging, This paper presents a novel method for realizing the field monitoring of channel siltation in real time. The method is based on the bistatic scattering theory and concerned more with the receiving and processing of multipath signal at high-frequency and small grazing angle. By use of the multipath propagation structure of underwater acoustic channel, the method obtains the silt thickness by calculating the relative time delay of acoustic signals between the direct and the shortest bottom reflected paths. Bistatic transducer pairs are employed to transmit and receive the acoustic signals, and the GPS time synchronization technology is introduced to synchronize the transmitter and receiver, The WRELAX (Weighted Fourier transforul and RELAX) algorithm is used to obtain the high resolution estimation of muhipath time delay. To examine the feasibility of the presented method and the accuracy and precision of the developed system, a series of sea trials are conducted in the southwest coast area of Dalian City, north of the Yellow Sea. The experimental results are compared with that using high-resolution dual echo sounder HydroBoxTM, and the uncertainty is smaller than + 0.06 m. Compared with the existing means for measuring the silt thickness, the present method is innovative, and the system is stable, efficient and provides a better real-time performance. It especially suits monitoring the narrow channel with rapid changes of siltation.展开更多
Klenow fragment(KF)uses the activity of a separate exonuclease to excise nucleotide,which is a crucial step in DNA replication and repair.Here is a novel sensitive and convenient method introduced for real-time monito...Klenow fragment(KF)uses the activity of a separate exonuclease to excise nucleotide,which is a crucial step in DNA replication and repair.Here is a novel sensitive and convenient method introduced for real-time monitoring nucleotide excision by KF with a molecular beacon as a detecting probe in a homogeneous solution.This method,which overcomes the drawbacks of traditional methods such as discontinuity,time consuming and low sensitivity,was used to assay KF activity and the detection limit reached up to 0.4 U/mL.In addition,the method was applied to investigating the effects of metal ions and chemical drugs on the reaction.The results demonstrate that it is a potential high-throughput assay for screening inhibitors and activity analysis of KF in vitro.展开更多
It is of great significance to develop an intelligent monitoring system for weld penetration defects such as incomplete penetration and burn-through in real-time during robotic arc welding process. In this paper, robo...It is of great significance to develop an intelligent monitoring system for weld penetration defects such as incomplete penetration and burn-through in real-time during robotic arc welding process. In this paper, robotic gas metal arc welding experiments are carried out on the mild steel test pieces with Vee-type groove. Through-the-arc sensing method is used to capture the transient values of the welding voltage and current. The raw data of the captured welding current and voltage are processed statistically, and the feature vector SIO is extracted to correlate the welding conditions to the weld penetration information. It lays foundation for intelligent monitoring of weld quality in robotic arc welding.展开更多
基金support of the National Natural Science Foundation of China(No.52274176)the Guangdong Province Key Areas R&D Program(No.2022B0101070001)+5 种基金Chongqing Elite Innovation and Entrepreneurship Leading talent Project(No.CQYC20220302517)the Chongqing Natural Science Foundation Innovation and Development Joint Fund(No.CSTB2022NSCQ-LZX0079)the National Key Research and Development Program Young Scientists Project(No.2022YFC2905700)the Chongqing Municipal Education Commission“Shuangcheng Economic Circle Construction in Chengdu-Chongqing Area”Science and Technology Innovation Project(No.KJCX2020031)the Fundamental Research Funds for the Central Universities(No.2024CDJGF-009)the Key Project for Technological Innovation and Application Development in Chongqing(No.CSTB2025TIAD-KPX0029).
摘要An innovative real-time monitoring method for surrounding rock damage based on microseismic time-lapse double-difference tomography is proposed for delayed dynamic damage identification and insufficient detection of adverse geological conditions in deep-buried tunnel construction.The installation techniques for microseismic sensors were optimized by mounting sensors at bolt ends which significantly improves signal-to-noise ratio(SNR)and anti-interference capability compared to conventional borehole placement.Subsequently,a 3D wave velocity evolution model that incorporates construction-induced disturbances was established,enabling the first visualization of spatiotemporal variations in surrounding rock wave velocity.It finds significant wave velocity reduction near the tunnel face,with roof and floor damage zones extending 40–50 m;wave velocities approaching undisturbed levels at 15 m ahead of the working face and on the laterally undisturbed side;pronounced spatial asymmetry in wave velocity distribution—values on the left side exceed those on the right,with a clear stress concentration or transition zone located 10–15 m;and systematically lower velocities behind the face than in front,indicating asymmetric rock damage development.These results provide essential theoretical support and practical guidance for optimizing dynamic construction strategies,enabling real-time adjustment of support parameters,and establishing safety early warning systems in deep-buried tunnel engineering.
摘要Fire emergencies have long posed a serious threat to people's lives,real estate assets,and environmental sustainability in civilized societies,especially when combustible events are detected at late stages of development.Recent advancements in computer vision-based fire detection have enabled automated real-time monitoring;however,most solutions either detect the existence of fire/smoke or employ binary decision-making,which limits visual monitoring systems from being risk-aware.This work introduces a severity-aware fire/smoke detection model that supports intelligent monitoring systems in detecting visual hazards.The goal is to identify varying levels of fire intensity and smoke density and to detect humans in real time.We design a system capable of monitoring environments using components such as sensing devices,network communication buses,cloud data centers,and computer visionbased detectors.The fire/smoke detection model comprises modern deep learning-based object detectors,with the YOLOvl2 model serving as the detection backbone.Moreover,our work proposes a two-stage training method that first learns coarse representations of fire,smoke,and humans,and then adapts the detector for fine-grained,severityaware classification,thereby enhancing severity discrimination and reducing inter-class confusion.We gathered our dataset to comprise approximately 65oo annotated images,split between coarse-grained and severity-aware detection models.The dataset consists of seven classes indicating human presence,three classes indicating varying levels of fire intensity,and three classes indicating varying levels of smoke density.We conducted experiments comparing three baseline object detection architectures(i.e.,YOLOvl2s,RT-DETR-L,and SSDLite320-MobileNetV3)using identical trainingesting configurations.YOLOvl2 has outperformed other baseline object detection architectures,achieving 0.929 mAP@50,0.884 precision,0.876 recall,0.880 F1-score,and 2.48 ms per-image latency,providing the best balance between detection performance and real-time processing capability.Our results indicate that severity-aware detection can improve the early-stage detection of intelligent monitoring systems.
摘要Tunnels are essential components of contemporary infrastructure,yet guaranteeing their safety,longevity,and efficiency remains a persistent challenge.Recent breakthroughs in artificial intelligence(AI)and digital twin(DT)technology provide innovative solutions for the real-time monitoring of tunnel systems,suggesting proactive maintenance tactics and improved safety protocols.This review paper offers a comprehensive examination of the application of AI and DT methodologies in tunnel surveillance.We explore the core concepts of AI and DT and their applicability to structural monitoring,encompassing machine learning,computer vision,and sensor integration.Through the utilization of these AI-powered technologies,engineers are equipped with unparalleled insights into the state and behavior of tunnels,facilitating the early identification of irregularities and the optimization of maintenance timelines.We discuss the array of AI techniques utilized for the immediate monitoring of tunnel systems,emphasizing their foundations,benefits,and practical uses.Numerous studies have showcased the effectiveness and adaptability of AI-based monitoring systems in various tunnel settings.Moreover,we address the hurdles and constraints inherent in AI and DT methodologies and suggest strategies for overcoming them,such as data augmentation,interpretable AI,edge computing,and continuous monitoring.Ultimately,the incorporation of AI and DT technologies into tunnel surveillance signifies a paradigm shift,offering substantial advantages over conventional techniques.By adopting AI-driven monitoring systems,tunnel operators can augment safety,prolong the lifespan of infrastructure,and decrease operational expenses,molding the future of subterranean infrastructure management.
摘要With the accelerated pace of urbanization, structural safety of buildings has garnered significant attention. Addressing the limitations of traditional monitoring methods—such as slow response times and delayed information transmission—researchers have conducted in-depth investigations into real-time building structure monitoring and developed an integrated monitoring system utilizing multiple sensor technologies. The study first examines various risks associated with prolonged building use, emphasizing the critical role of real-time monitoring systems in early defect detection and maintenance management. By employing sensor network technology, the system successfully collects and continuously monitors real-time data on structural vibrations, temperature fluctuations, and humidity levels. Through systematic integration and optimized data acquisition modules, all monitoring data are promptly transmitted to designated locations for immediate visualization. Practical testing demonstrated the system's high accuracy and reliability in rapid response and early fault identification, along with robust adaptability and scalability. These findings not only enhance the intelligence of building structural safety monitoring technologies but also provide robust theoretical foundations and practical guidelines for proactive maintenance and risk mitigation, thereby extending building service life while significantly reducing maintenance costs. This comprehensive planning scheme effectively safeguards building safety while significantly enhancing the operational efficiency of monitoring systems. It holds substantial practical value for widespread application and plays a crucial theoretical and practical role in accelerating the digital transformation of building management and further improving the overall security standards of urban infrastructure.
基金supported by the Student’s Innovation Capability Improvement Plan Project of Guangzhou Medical University(2023 to B.L.)Guangzhou science and technology plan projects[2023A03J0400 to W.C.O and 2024A03J0940 to B.L.]Guangdong Basic and Applied Basic Research Foundation[2021A1515011364 to W.L.].
摘要Objectives To develop and validate a machine learning(ML)model for real-time monitoring of in-hospital mortality(IHM)risk and identification of major risk factors in heart failure(HF)patients admitted to intensive care units(ICUs).Methods Data from ICU HF patients were extracted from the multicenter eICU-Collaborative Research Database(eICU-CRD.External validation used MIMIC-IV and a real-world Chinese dataset(CHN-dataset).Daily measurements from MIMIC-IV patients staying≥3 days formed a Daily Measurement(DM)dataset.After rigorous preprocessing and feature selection,five ML algorithms were trained and optimized using eICU-CRD data.Model performance was evaluated using AUC,sensitivity,specificity,and balanced accuracy.The optimal model was benchmarked against APACHE and SOFA scores.SHapley Additive ex-Planations(SHAP)interpreted feature contributions.A Windows application was developed for clinical deployment.Results XGBoost emerged as the optimal model(Final-ML model),which incorporated only 17 routinely collected clinical variables:age,non-invasive systolic blood pressure(NI-SBP),heart rate,respiratory rate,Glasgow Coma Scale eye opening score,white blood cell count(WBC),creatinine,bicarbonate,red cell distribution width(RDW),platelet count,glucose,calcium,mean corpuscular hemoglobin concentration(MCHC),sodium,mean corpuscular volume,red blood cell count,and potassium.It achieved high AUCs:0.876(95%CI:0.836-0.915;eICU-CRD test data),0.932(95%CI:0.921-0.942;MIMIC-IV),and 0.879(95%CI:0.846-0.912;CHN-dataset).It significantly outperformed APACHE(AUC=0.740,95%CI:0.720-0.761)and SOFA(AUC=0.717,95%CI:0.694-0.740)scores.The model demonstrated strong generalizability across ethnicities,ward types,and genders within MIMIC-IV.Using daily data(DM dataset),predicted IHM risk accurately tracked patient trajectories:risk decreased progressively for survivors and increased for non-survivors throughout the ICU stay.SHAP analysis identified key predictors:NI-SBP,age,heart rate,WBC,glucose,and notably,RDW and MCHC.Time-dependent Cox regression confirmed RDW increase(HR=3.783,95%CI:2.237-6.398)and MCHC decrease(HR=0.173,95%CI:0.040-0.741)as significant independent risk factors for IHM.Conclusions The developed XGBoost model provides a reliable,generalizable tool for real-time IHM risk quantification and monitoring in ICU HF patients,using only 17 routinely collected clinical variables.It surpasses traditional scoring systems and enables dynamic risk assessment throughout the ICU stay.By identifying patient-specific major risk factors via SHAP values,the model facilitates timely,personalized treatment adjustments.
基金supported by the National Institute of Health(R37CA240806,U01CA288351,and R50CA283816)support from UCI Chao Family Comprehensive Cancer Center(P30CA062203).
摘要Electroacoustic Tomography(EAT)is an imaging technique that detects ultrasound waves induced by electrical pulses,offering a solution for real-time electroporation monitoring.This study presents EAT system using a dual-frequency ultrasound array.The broadband nature of electroacoustic signals requires ultrasound detector to cover both the high-frequency range(around 6MHz)signals generated by small targets and the low-frequency range(around 1MHz)signals generated by large targets.In our EAT system,we use the 6 MHz array to detect high-frequency signals from the electrodes,and the 1 MHz array for the electrical field.To test this,we conducted simulations using COMSOL Multiphysics® and MATLAB's k-Wave toolbox,followed by experiments using a custom-built setup with a dual-frequency transducer and real-time data acquisition.The results demonstrated that the dual-frequency EAT system could accurately and simultaneously monitor the electroporation process,effectively showing both the treatment area and electrode placement with the application of 1 kV electric pulses with 100 ns duration.The axial resolution of the 6MHz array for EAT was 0.45 mm,significantly better than the 2mm resolution achieved with the 1MHz array.These findings validate the potential of dual-frequency EAT as a superior method for real-time electroporation monitoring.
基金supported by Jiangsu Innovation Team Program,China,National Natural Science Foundation of China(Grant Nos.:82204339,82473884,82122066,and 81973291)National Key Research and Development Program of Ministry of China(Grant No.:2022YFC2704603)+1 种基金the“Dawn”program of Shanghai Education Commission,China(Grant No.:22SG34)Natural Science Foundation of Sichuan Province of China(Grant No.:2023NSFSC1902).
摘要Acetylcholinesterase(AChE)plays a crucial role in the activities of the nervous system,and its abnormal function can lead to the occurrence and development of neurodegenerative diseases.Hence,an effective method for real-time monitoring of AChE activity is essential.Very recently,several fluorescence sensors have been developed for the detection of AChE activity,but they are usually imaging in the visible region,relatively small Stokes shifts,or long response times,limiting their application for real-time monitoring in vivo.Inspired by that,a near-infrared(NIR)off-on probe((E)-4-(2-(4-(dicyanomethylene)-4H-chromen-2-yl)vinyl)phenyl dimethylcarbamate,DCM-N)for AChE monitoring with high selectivity and sensitivity is developed.In the probe DCM-N,a bright near-infrared fluorescence emission at 700 nm can be triggered by AChE through the cleavage of amino ester bond in DCM-N,and the resulting fluorescence exhibits a good linear relationship with AChE activity in the range of 0.2–16 U/mL,with a detection limit as low as 0.06 U/mL.For real plasma sample detection,DCM-N demonstrates advantages of accurate detection and fast response compared to the traditional Ellman assay for AChE detection.Moreover,DCM-N can be used for imaging of AChE activity in live cells and tracking of AChE activity in zebrafish models,which is of great significance for medical and physiological research related to AChE.DCM-N possesses several notable features such as light-up NIR emission,fast response,large spectral shifts and strong photostability under physiological conditions.These features enable it to monitor AChE activity both in vivo and in vitro,providing a suitable tool for real-time monitoring and in vivo visualization of AChE activity.
摘要Large portions of the tunnel boring machine(TBM)construction cost are attributed to disc cutter consumption,and assessing the disc cutter's wear level can help determine the optimal time to replace the disc cutter.Therefore,the need to monitor disc cutter wear in real-time has emerged as a technical challenge for TBMs.In this study,real-time disc cutter wear monitoring is developed based on sound and vibration sensors.For this purpose,the microphone and accelerometer were used to record the sound and vibration signals of cutting three different types of rocks with varying abrasions on a laboratory scale.The relationship between disc cutter wear and the sound and vibration signal was determined by comparing the measurements of disc cutter wear with the signal plots for each sample.The features extracted from the signals showed that the sound and vibration signals are impacted by the progression of disc wear during the rock-cutting process.The signal features obtained from the rock-cutting operation were utilized to verify the machine learning techniques.The results showed that the multilayer perceptron(MLP),random subspace-based decision tree(RS-DT),DT,and random forest(RF)methods could predict the wear level of the disc cutter with an accuracy of 0.89,0.951,0.951,and 0.927,respectively.Based on the accuracy of the models and the confusion matrix,it was found that the RS-DT model has the best estimate for predicting the level of disc wear.This research has developed a method that can potentially determine when to replace a tool and assess disc wear in real-time.
基金“Research on AI-Intelligent Management Technology for Construction Safety Based on BIM Technology and Smart Construction Site Scenarios”(Project No.:KJQN202401904)“Research on Intelligent Monitoring System for Construction Quality and Safety Based on BIM and AI Technologies”(Project No.:202412608006)。
摘要Combining the background of modern construction engineering site safety management,this article analyzes the real-time monitoring and alarm strategies for site construction safety under the integration of BIM and AI.This includes the analysis of BIM and AI technologies and their integration advantages,real-time monitoring and alarm strategies for construction site safety based on BIM and AI integration,as well as the development direction of BIM and AI integration in real-time monitoring and alarm for construction site safety.It is hoped that through this analysis,a scientific reference can be provided for the digital and intelligent management of construction site safety,promoting the digital and intelligent development of its safety management work.
摘要The Internet of Things technology provides a comprehensive solution for the real-time monitoring of cold chain logistics by integrating sensors,wireless communication,cloud computing,and big data analysis.Based on this,this paper deeply explores the overview and characteristics of the Internet of Things technology,the feasibility analysis of the Internet of Things technology in the cold chain logistics monitoring,the application analysis of the Internet of Things technology in the cold chain logistics real-time monitoring to better improve the management level and operational efficiency of the cold chain logistics,to provide consumers with safer and fresh products.
基金possible by the US Army Medical Research and Development Command through the Medical Technology Enterprise Consortium under Contract#W81XWH-15-9-0001.
摘要Bioreactors are used to dynamically condition engineered tissues to achieve the required degree of maturation before in vivo implantation.Integrating sensors and imaging capabilities into bioreactors can help us understand how the culture environment influences tissue maturation and growth.Additionally,this enables the monitoring of tissue constructs and provides critical information for quality control.This study aimed to develop a standardized,self-contained,uniaxial bioreactor module for the clinical manufacturing of tissue constructs;this system would benefit from unidirectional mechanical or electrical stimulation,or both.We achieved this goal by integrating stimulation and sensing components that provide an optimal culture environment and monitoring capabilities to improve tissue manufacturing.The uniaxial bioreactor module included integrated,user-friendly mechanical and electrical stimulations with force measurement to enhance the preconditioning of the engineered tissues.Also,a sensor loop and media exchange system were integrated to monitor the culture environment and cellular metabolites over time,and the camera system above the tissue construct enabled the macroscopic visualization of tissue maturation.Furthermore,the onboard media exchange system was programmed into the module to maintain aseptic culture conditions in the long term.Subsequently,using native skeletal muscle tissue and tissue-engineered skeletal muscle constructs,the performance of the uniaxial bioreactor module was validated for its application in preconditioning and enhancing tissue maturation.
基金Project supported by the Innovative Research Groups of the National Natural Science Foundation of China (No. 51621092), the National Basic Research Program (973 Program) of China (No. 2013CB035904), and the Natural Science Foundation of China (No. 51439005)
摘要Time, cost, and quality are three key control factors in rockfill dam construction, and the tradeoff among them is important. Research has focused on the construction time-cost-quality tradeoff for the planning or design phase, built on static empirical data. However, due to its intrinsic uncertainties, rockfill dam construction is a dynamic process which requires the tradeoffto adjust dynamically to changes in construction conditions. In this study, a dynamic time-cost-quality tradeoff (DTCQT) method is proposed to balance time, cost, and quality at any stage of the construction process. A time-cost-quality tradeoff model is established that considers time cost and quality cost. Time, cost, and quality are dynamically estimated based on real-time monitoring. The analytic hierarchy process (AHP) method is applied to quantify the decision preferences among time, cost, and quality as objective weights. In addition, an improved non-dominated sorting genetic algorithm (NSGA-II) coupled with the technique for order preference by similarity to ideal solution (TOPSIS) method is used to search for the optimal compromise solution. A case study project is analyzed to demonstrate the applicability of the method, and the efficiency of the proposed optimization method is compared with that of the linear weighted sum (LWS) and NSGA-II.
摘要[Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been proposed for monitoring cow ruminant behavior,including video surveillance,sound recognition,and sensor monitoring methods.How‐ever,the application of edge device gives rise to the issue of inadequate real-time performance.To reduce the volume of data transmission and cloud computing workload while achieving real-time monitoring of dairy cow rumination behavior,a real-time monitoring method was proposed for cow ruminant behavior based on edge computing.[Methods]Autono‐mously designed edge devices were utilized to collect and process six-axis acceleration signals from cows in real-time.Based on these six-axis data,two distinct strategies,federated edge intelligence and split edge intelligence,were investigat‐ed for the real-time recognition of cow ruminant behavior.Focused on the real-time recognition method for cow ruminant behavior leveraging federated edge intelligence,the CA-MobileNet v3 network was proposed by enhancing the MobileNet v3 network with a collaborative attention mechanism.Additionally,a federated edge intelligence model was designed uti‐lizing the CA-MobileNet v3 network and the FedAvg federated aggregation algorithm.In the study on split edge intelli‐gence,a split edge intelligence model named MobileNet-LSTM was designed by integrating the MobileNet v3 network with a fusion collaborative attention mechanism and the Bi-LSTM network.[Results and Discussions]Through compara‐tive experiments with MobileNet v3 and MobileNet-LSTM,the federated edge intelligence model based on CA-Mo‐bileNet v3 achieved an average Precision rate,Recall rate,F1-Score,Specificity,and Accuracy of 97.1%,97.9%,97.5%,98.3%,and 98.2%,respectively,yielding the best recognition performance.[Conclusions]It is provided a real-time and effective method for monitoring cow ruminant behavior,and the proposed federated edge intelligence model can be ap‐plied in practical settings.
基金supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIT) (No.2022M3J7A1062940,2021R1A5A6002853,and 2021R1A2C3011585)supported by the Technology Innovation Program (20015577)funded by the Ministry of Trade,Industry&Energy (MOTIE,Korea)。
摘要This review explores glucose monitoring and management strategies,emphasizing the need for reliable and userfriendly wearable sensors that are the next generation of sensors for continuous glucose detection.In addition,examines key strategies for designing glucose sensors that are multi-functional,reliable,and cost-effective in a variety of contexts.The unique features of effective diabetes management technology are highlighted,with a focus on using nano/biosensor devices that can quickly and accurately detect glucose levels in the blood,improving patient treatment and control of potential diabetes-related infections.The potential of next-generation wearable and touch-sensitive nano biomedical sensor engineering designs for providing full control in assessing implantable,continuous glucose monitoring is also explored.The challenges of standardizing drug or insulin delivery doses,low-cost,real-time detection of increased blood sugar levels in diabetics,and early digital health awareness controls for the adverse effects of injectable medication are identified as unmet needs.Also,the market for biosensors is expected to expand significantly due to the rising need for portable diagnostic equipment and an ever-increasing diabetic population.The paper concludes by emphasizing the need for further research and development of glucose biosensors to meet the stringent requirements for sensitivity and specificity imposed by clinical diagnostics while being cost-effective,stable,and durable.
基金National Key Technology R&D Program in the 12th Five Year Plan of China (No. 2011BAB10B06)Independent Innovation Foundation of Tianjin University (No. 1102119)
摘要The theory and method of system integration for the real-time monitoring of core rock-fill dam filling con- struction quality are studied in this paper. First, the importance analysis of system integration factors is carried out with the analytic hierarchy process. Then, according to the analysis result of integration factors, the conceptual model of system integration is built based on function integration, index integration, technology integration and information integration, the index structure of core rock-fill dam filling construction quality control is constructed and the method of function integration and technology integration is studied. The mathematical model of process monitoring is built according to monitoring objective, process and indexes. Research results have been applied in Nuozhadu core rock-fill dam construction management, realizing system integration through building appropriate monitoring work flow and comprehensive information platform of digital dam.
基金Meg-science Program of the Chinese Academy of Sciences (No. 19981303)
摘要A new system called alternate data acquisition and real-time monitoring system has been developed for long-time discharge in tokamak operation. It can support continuous on-line data acquisition at a high sampling rate and a graphic display of the plasma parameters during the discharge. Thus operators can monitor and control the plasma state in real time. An application of this system has been demonstrated on the HT-7 tokamak.
摘要As global climate change intensifies,the power industry-a major source of carbon emissions-plays a pivotal role in achieving carbon peaking and neutrality goals through its low-carbon transition.Traditional power plants’carbon management systems can no longer meet the demands of high-precision,real-time monitoring.Smart power plants now offer innovative solutions for carbon emission tracking and intelligent analysis by integrating IoT,big data,and AI technologies.Current research predominantly focuses on optimizing individual processes,lacking systematic exploration of comprehensive dynamic monitoring and intelligent decision-making across the entire workflow.To address this gap,we propose a smart carbon emission monitoring and analysis platform for power plants that integrates IoT sensing,multimodal data analytics,and AI-driven decision-making.The platform establishes a multi-source sensor network to collect emissions data throughout the fuel combustion,auxiliary equipment operation,and waste treatment processes.Combining carbon emission factor analysis with machine learning models enables real-time emission calculations and utilizes long short-term memory networks to predict future emission trends.
基金supported by the National Key Technology Research and Development Program of China(863 Program, Grant No.2009BAG18B03)
摘要Monitoring the thickness changes of channel siltation is paramount in safeguarding navigation and guiding dredging, This paper presents a novel method for realizing the field monitoring of channel siltation in real time. The method is based on the bistatic scattering theory and concerned more with the receiving and processing of multipath signal at high-frequency and small grazing angle. By use of the multipath propagation structure of underwater acoustic channel, the method obtains the silt thickness by calculating the relative time delay of acoustic signals between the direct and the shortest bottom reflected paths. Bistatic transducer pairs are employed to transmit and receive the acoustic signals, and the GPS time synchronization technology is introduced to synchronize the transmitter and receiver, The WRELAX (Weighted Fourier transforul and RELAX) algorithm is used to obtain the high resolution estimation of muhipath time delay. To examine the feasibility of the presented method and the accuracy and precision of the developed system, a series of sea trials are conducted in the southwest coast area of Dalian City, north of the Yellow Sea. The experimental results are compared with that using high-resolution dual echo sounder HydroBoxTM, and the uncertainty is smaller than + 0.06 m. Compared with the existing means for measuring the silt thickness, the present method is innovative, and the system is stable, efficient and provides a better real-time performance. It especially suits monitoring the narrow channel with rapid changes of siltation.
基金Supported by the Key Project of Natural Science Foundation of China(No.90606003)the Hunan Provincial Natural Science Foundation,China(No.08JJ1002)+1 种基金the National High-Tech Research and Development Program(No.2007AA022007)the Changjiang Scholars and Innovative Research Team in University,China
摘要Klenow fragment(KF)uses the activity of a separate exonuclease to excise nucleotide,which is a crucial step in DNA replication and repair.Here is a novel sensitive and convenient method introduced for real-time monitoring nucleotide excision by KF with a molecular beacon as a detecting probe in a homogeneous solution.This method,which overcomes the drawbacks of traditional methods such as discontinuity,time consuming and low sensitivity,was used to assay KF activity and the detection limit reached up to 0.4 U/mL.In addition,the method was applied to investigating the effects of metal ions and chemical drugs on the reaction.The results demonstrate that it is a potential high-throughput assay for screening inhibitors and activity analysis of KF in vitro.
摘要It is of great significance to develop an intelligent monitoring system for weld penetration defects such as incomplete penetration and burn-through in real-time during robotic arc welding process. In this paper, robotic gas metal arc welding experiments are carried out on the mild steel test pieces with Vee-type groove. Through-the-arc sensing method is used to capture the transient values of the welding voltage and current. The raw data of the captured welding current and voltage are processed statistically, and the feature vector SIO is extracted to correlate the welding conditions to the weld penetration information. It lays foundation for intelligent monitoring of weld quality in robotic arc welding.