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Increasing the Response Speed Without Redesigning the System:A Reference Input Scheduling Approach 认领 引用
1
作者 Zongli Lin 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2026年第1期1-2,共2页
WE observe that the response speed of a linear timeinvariant system to a step reference input depends not only on the system parameters but also on the magnitude of the step input.Based on this observation,we demonstr... WE observe that the response speed of a linear timeinvariant system to a step reference input depends not only on the system parameters but also on the magnitude of the step input.Based on this observation,we demonstrate a method to schedule the magnitude of the reference input to achieve a faster response. 展开更多
关键词 schedule magnitude reference input reference input scheduling linear timeinvariant system response speed linear time invariant system step input system parameters step reference input
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Optimal beamwidth design of periodic reference signals for integrated sensing and communications in cellular V2X networks 认领 引用
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作者 Chunlei Sun Xuqing Liu +3 位作者 Linpei Li Yuzheng Ren Dongzhe Li Haijun Zhang 《Digital Communications and Networks》 SCIE EI CSCD 2026年第3期550-559,共10页
Recently,the technique of Integrated Sensing and Communication(ISAC)has gained great attentions and is expected to enable more advanced applications in cellular Vehicle-to-Everything(V2X).We intend to use the periodic... Recently,the technique of Integrated Sensing and Communication(ISAC)has gained great attentions and is expected to enable more advanced applications in cellular Vehicle-to-Everything(V2X).We intend to use the periodical reference signals,such as the synchronization signals in 5G,to actively detect targets at different azimuths via beam scanning.This paper mainly aims to optimize the beamwidth of these reference signals in cellular-V2X to facilitate both sensing and communication functions.Firstly,to address the issue of insufficient beam duration for accurate estimation of Doppler and the corresponding velocity as well,we combine multi-ple beam scanning cycles to support improved sensing performance.In this context,an innovative beamwidth optimization algorithm is proposed.Specifically,we deduce the Fisher Information Matrix(FIM)of the suffi-cient statistic associated with target azimuths.Considering the randomness of the target position,we build the objective function based on the expected trace of the derived FIM.Additionally,we formulate the constraint of channel coherent time for efficient communications.It is worth noting that this is the first time to optimize the beamwidth of periodic reference signals for joint communication and sensing in cellular V2X.Experimental results show that the optimal beamwidth varies with the steering azimuth of antennas and reaches its maximum when the beam points at the direction perpendicular to the roadside. 展开更多
关键词 Integrated sensing and communications Cellular V2X Periodic reference signals Beamwidth optimization 5G
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Collaborative ISAC Mechanism for Base Station and Users with Reference Signals 认领 引用
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作者 Sun Chunlei Wang Zhigang +5 位作者 Ren Yuzheng Liu Xuqing Qu Aixi Sun Chen Li Haojin Zhang Haijun 《China Communications》 SCIE EI CSCD 2026年第3期182-198,共17页
Integrated sensing and communication(IS AC)is emerging as a key technology for future cellular networks.This paper focuses on the collaborative ISAC mechanism between base stations(BSs)and users using reference signal... Integrated sensing and communication(IS AC)is emerging as a key technology for future cellular networks.This paper focuses on the collaborative ISAC mechanism between base stations(BSs)and users using reference signals(RSs).The main challenges we address are the joint optimization of downlink communication and sensing resources,the selection of users as sensing anchors,as well as the fusion of estimation data between the BS and users.We formulate the collaborative ISAC problem as a multiobjective programming framework,which can balance system performance in sensing and individual benefits in communication.Particularly,to ensure fairness,we propose minimizing the largest sensing age across all users.On this basis,we put forward an efficient solution algorithm that enables a low-complexity computation of the Pareto front when it exists.Simulation results demonstrate that the proposed collaborative ISAC mechanism is capable of efficiently enhancing the system’s sensing capacity while ensuring fairness in user scheduling for sensing. 展开更多
关键词 collaborative ISAC integrated sensing and communication reference signals resource scheduling sensing anchor selection
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Identifying reliable reference genes for qPCR in skinks under different thermal stress 认领 引用
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作者 Ziwen Yang Lining Chen +4 位作者 Wenning Zhang Huiyuan Wu Jiehong Ji Jiayong Zhang Danna Yu 《Zoological Systematics》 CSCD 2026年第2期150-162,共13页
Global warming has emerged as the predominant trend in global climate change,with forecasts suggesting a rise in the occurrence and severity of extreme weather phenomena.As a result,ectothermic animals have become ide... Global warming has emerged as the predominant trend in global climate change,with forecasts suggesting a rise in the occurrence and severity of extreme weather phenomena.As a result,ectothermic animals have become ideal model organisms for investigating the potential impacts of climate change on global biodiversity.Real-time fluorescence quantitative PCR(RT-qPCR)is a widely used technique for gene expression analysis.However,stable reference genes suitable for accurate normalization of transcriptional data across lizard samples have not yet been established.In this study,three skink species-Scincella modesta,S.reevesii,and Ateuchosaurus chinensis-were selected as model organisms to evaluate the expression stability of eight candidate housekeeping genes(18S rRNA,EF1α,ACTB,GAPDH,HPRT1,YWHAZ,RPS15,and RPS18)under nine distinct temperature conditions(6℃,10℃,14℃,18℃,22℃,25℃,28℃,32℃,and 36℃).The Delta Ct method,BestKeeper,NormFinder,GeNorm,and RefFinder were utilized to assess expression consistency and to identify the most suitable reference genes,as well as the ideal number needed for reliable normalization.The results indicated that under varying temperature conditions,the best-performing internal controls were RPS18 and RPS15 for S.modesta;RPS18 and EF1α for S.reevesii;and RPS15 and YWHAZ for A.chinensis.Furthermore,RPS18,RPS15,and EF1α were identified as a stable and commonly applicable set of internal controls for RT-qPCR normalization across all three skink species and are recommended as reliable reference genes for use in related lizards exposed to thermal stress. 展开更多
关键词 Skink normalization reference genes RefFinder
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LPF-free Harmonic Current Control in Multi-synchronous Reference Frames for Dual Three-phase PMSMs 认领 引用
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作者 Yulei Yang Qian Wang +1 位作者 Fangrui Wei Yichen Yu 《CES Transactions on Electrical Machines and Systems》 EI CSCD 2026年第2期216-227,共12页
Harmonic current regulation in multi-phase motor drives remains challenging under dynamic operating conditions due to non-sinusoidal back electromotive force(EMF)and inverter nonlinearities,which introduce dominant lo... Harmonic current regulation in multi-phase motor drives remains challenging under dynamic operating conditions due to non-sinusoidal back electromotive force(EMF)and inverter nonlinearities,which introduce dominant low-order harmonics.Multi-synchronous reference frame(MSRF)based methods are widely adopted for multi-frequency current regulation;however,conventional implementations typically rely on low-pass filters(LPFs)for harmonic decoupling,introducing detection delay and phase distortion that limit transient performance.This paper proposes an LPF-free MSRF-based harmonic current control method for dual three-phase permanent magnet synchronous motor(DTP-PMSM)drives.By introducing harmonic transformation matrix coefficients(HTMCs),the proposed method retains the independent regulation of multiple harmonic components within the MSRF framework,while eliminating the filtering step in the harmonic decoupling path,without the need for harmonic plane reconstruction or additional coordinate transformations.To support adaptive changes,a Goertzel-based harmonic amplitude extraction scheme is employed to periodically update the HTMC.Experimental results demonstrate that the proposed method significantly enhances dynamic performance,achieving a 19.3% reduction in speed transition stabilization time compared to other methods.The proposed LPF-free formulation provides a practical solution for MSRF-based harmonic current control in industrial motor drive applications where fast dynamic response is required. 展开更多
关键词 Low-pass filter(LPF)-free harmonic decoupling Multi-synchronous reference frame(MSRF) Multi-frequency current control Harmonic current suppression Industrial motor drives Dual three-phase permanent magnet synchronous motors(DTP-PMSMs)
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RAGA:a reference-assisted genome assembly tool for efficient population-scale assembly 认领 引用
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作者 Ru-Peng Zhao Yu-Hong Luo +9 位作者 Wen-Zhao Xie Zu-Wen Zhou Yong-Qing Qian Si-Long Yuan Dong-Ao Li Jiana Li Kun Lu Xingtan Zhang Jia-Ming Song Ling-Ling Chen 《Horticulture Research》 SCIE CSCD 2025年第11期123-134,共12页
High-quality reference genomes at the population scale are fundamental for advancing pan-genomic research.However,high-quality genome assembly at the population scale is costly and time-consuming.To overcome these lim... High-quality reference genomes at the population scale are fundamental for advancing pan-genomic research.However,high-quality genome assembly at the population scale is costly and time-consuming.To overcome these limitations,we developed ReferenceAssisted Genome Assembly(RAGA),a hybrid computational tool that combines de novo and reference-based assembly approaches.RAGA efficiently employs existing reference genomes from the same or closely related species in combination with PacBio HiFi reads to produce high-quality alternative long sequences.These sequences can be integrated with de novo assemblies to improve assembly quality across population-scale datasets.The performance of RAGA across various plant genomes demonstrated its ability to reduce the number of contigs,decrease gaps,and correct genome assembly errors.The implementation of RAGA(available at http://gffzz188fe103f8f1460asu55u0kv55nfx6qu9.ffgz.tsg.suse.edu.cn/wzxie/RAGA)significantly streamlines population-scale genome assembly workf lows,providing a robust foundation for comprehensive pan-genomic investigations.This tool represents a substantial advancement in making large-scale genomic studies more accessible and efficient. 展开更多
关键词 hybrid computational tool referenceassisted genome assembly raga population scale genome assembly pacbio hifi reads reference assisted assembly high quality reference genomes reference genomes pan genomic research
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Systematic evaluation of reference gene stability across tissues and sexes in cuttlefish(Sepiella japonica) 认领 引用
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作者 LI Shuang GU Min +5 位作者 YE Xiaowen YE Bin LIU Siyu YU Jia ZHOU Xu CHI Changfeng 《水产学报》 CAS CSCD 北大核心 2026年第2期26-49,共24页
Quantitative real-time PCR(qPCR)is widely used for gene expression analysis,but its accuracy critically depends on stable internal reference genes for normalization.In marine invertebrates,especially non-model taxa su... Quantitative real-time PCR(qPCR)is widely used for gene expression analysis,but its accuracy critically depends on stable internal reference genes for normalization.In marine invertebrates,especially non-model taxa such as cephalopods,systematic evaluation of reference genes is limited,leading to potential bias.The cuttlefish Sepiella japonica is ecologically and economically important in China,yet previous molecular studies have often relied on single unvalidated reference genes,which may compromise data reliability.This study aimed to systematically evaluate the stability of five commonly used reference genes(18S,ef-1α,ef-1γ,gapdh,andβ-actin)across multiple tissues and sexes of S.japonica,and to identify the most suitable reference genes and optimal number for qPCR normalization.Fifteen to sixteen tissue types were collected from ten healthy adults(five males and five females).Total RNA was extracted,reverse-transcribed,and analyzed by qPCR.Gene stability was assessed using four algorithms(geNorm,NormFinder,BestKeeper,andΔCt)integrated with RefFinder,and the optimal gene number was determined using geNorm pairwise variation(Vn+1ef-1α>18S>gapdh>β-actin(male)and ef-1α>ef-1γ>18S>gapdh>β-actin(female).geNorm analysis(V2/3<0.15)indicated that two genes,mainly ef-1αand ef-1γ,were generally sufficient for reliable normalization in most tissues.Validation confirmed that normalization using the stable ef-1αand ef-1γaccurately reflected the expression differences among tissues,whereasβ-actin and gapdh can bias or confound statistical analyses.ef-1αand ef-1γare identified as the most reliable reference gene combination for qPCR analysis in S.japonica,while 18S can serve as an auxiliary gene for within-sex comparisons.The use ofβ-actin or gapdh alone is not recommended.This study establishes a systematic framework for selecting reliable reference genes in S.japonica,thereby facilitating robust qPCR normalization and providing a foundation for future gene expression research in S.japonica and other cephalopods. 展开更多
关键词 Sepiella japonica reference genes qPCR expression stability RefFinder
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p-GaN HEMT current reference and current mirror for high temperature application 认领 引用
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作者 Pingyu Cao Kepeng Zhao +5 位作者 Zhengxuan Li Yihao Xu Ping Zhang Harm Van Zalinge Miao Cui Fei Xue 《Journal of Semiconductors》 EI CAS CSCD 2026年第4期93-101,共9页
This paper demonstrates a monolithically integrated current reference and current mirror based on p-GaN gate HEMT technology,designed for high-temperature applications.The p-GaN current reference is composed of one D-... This paper demonstrates a monolithically integrated current reference and current mirror based on p-GaN gate HEMT technology,designed for high-temperature applications.The p-GaN current reference is composed of one D-mode and two E-mode devices.The generated reference current is independent of supply voltage since the proposed circuit incorporates a bias circuit capable of providing a supply-voltage-insensitive bias voltage.Moreover,under the zero-temperature coefficient(ZTC)bias voltage condition,the variation in the generated reference current at 200℃ is reduced by 15.4%,compared to a conventional p-GaN current reference with a bias voltage of 5 V.Experimental results indicate that the generated reference current slightly reduced from 2.53 to 1.70 mA over a broad temperature range of 25-200℃.In addition,a current mirror circuit based on p-GaN HEMT technology was designed to imitate a reference current.The influence of temperature on the output current of the current mirror is mitigated,which could be realised by biasing the gate-to-source voltage at the zero-temperature coefficient voltage.This design sustains the current mirror mismatch error with small variation across a temperature range from room temperature to 200℃.These results indicate that the GaN current reference and current mirror under zero-temperature coefficient bias voltage can ensure stable output current across different temperatures,facilitating the application of fully GaN integrated circuits in high-temperature environments. 展开更多
关键词 p-GaN HEMTs current reference current mirror high-temperature application supply voltage insensitivity monolithic integration
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Peer-to-Peer IoT Authentication Protocol Based on PUF and Multiple Reference Fuzzy Extractor 认领 引用
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作者 Qingyao Gu Mengqi Hu +2 位作者 Zerui Zhao Liquan Chen Huiyu Fang 《Computers, Materials & Continua》 SCIE EI 2026年第7期321-339,共19页
With the rapid development of the Internet of Things(IoT),the widespread adoption of applications such as smart homes and industrial IoT has raised the demand for secure authentication and key agreement among resource... With the rapid development of the Internet of Things(IoT),the widespread adoption of applications such as smart homes and industrial IoT has raised the demand for secure authentication and key agreement among resource-constrained devices over open communication channels.Traditional authentication protocols often rely on centralized servers for key distribution,which results in high communication overhead and exposes systems to single-point-of-failure risks.Moreover,IoT devices are typically constrained in computational resources and are vulnerable to hardware cloning.These limitations necessitate lightweight yet robust security mechanisms.To address these challenges,we propose a lightweight peer-to-peer authentication protocol based on Physically Unclonable Function(PUF)and Multiple Reference Fuzzy Extractor(MRFE).The proposed protocol enables direct mutual authentication and key agreement between IoT devices without the participation of a trusted third-party server.Formal security analysis,along with evaluations of computation and communication costs,demonstrates that the protocol achieves strong security guarantees while maintaining high efficiency.Therefore,the proposed protocol is well-suited for lightweight peer-to-peer authentication scenarios in IoT environments. 展开更多
关键词 Internet of Things physically unclonable function multiple reference fuzzy extractor peer-to-peer authentication
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Assessing the Performance of 8 AI Chatbots in Bibliographic Reference Retrieval:Grok and DeepSeek Outperform ChatGPT,but None are Entirely Accurate 认领 引用
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作者 Álvaro Cabezas-Clavijo Pavel Sidorenko-Bautista 《Journal of Data and Information Science》 CSCD 2026年第2期102-114,共13页
Purpose:This study evaluates the reliability of eight generative artificial intelligence chatbots—including ChatGPT,Claude,Gemini,and DeepSeek—when functioning as autonomous agents for academic bibliographic generat... Purpose:This study evaluates the reliability of eight generative artificial intelligence chatbots—including ChatGPT,Claude,Gemini,and DeepSeek—when functioning as autonomous agents for academic bibliographic generation,specifically assessing their accuracy within a university research framework.Design/methodology/approach:Using a standardized prompting methodology,400 references were generated and analyzed across five core knowledge areas:Health,Engineering,Experimental Sciences,Social Sciences,and Humanities.Each agent’s output was rigorously audited against five formal criteria(authorship,year,title,source,and location)and categorized by error frequency and document type.Findings:Results indicate a significant reliability gap,with only 26.5%of references entirely accurate and nearly 40%flawed or fabricated;while Grok and DeepSeek avoided hallucinations,Copilot,Perplexity,and Claude showed the highest failure rates,particularly when generating journal article citations.Research limitations:The study focuses on the free versions of these AI agents,so results may vary with paid models or future architectural updates that integrate realtime web browsing more effectively.Practical implications:These findings underscore the critical risks of uncritical reliance on AI agents for academic tasks,highlighting an urgent need for enhanced information literacy and the development of specialized critical thinking skills to navigate AI-mediated research.Originality/value:This original and unpublished research provides a pioneering comparative analysis of multiple AI agents as research intermediaries,revealing structural limitations in their generative logic and offering a unique benchmark for the reliability of AI-driven bibliographic data in higher education. 展开更多
关键词 AI chatbots bibliographic references information literacy hallucination detection academic integrity higher education
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无愧于“参考”之名 聆听ROCKNA Wavedream Reference解码器/数字播放器有感 认领 引用
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作者 剑萍 《视听前线》 2026年第7期2-6,共5页
FPGA和R2R阵列目前,ROCKNA这个罗马尼亚品牌在Hi-End数字音频界如日中天,它的解码器、数字转盘/服务器在发烧圈中拥有崇高的地位,而更令发烧友感兴趣的是--和其它Hi-End产品相比,ROCKNA的价格并不属于高不可攀的类型。ROCKNA的创始人和... FPGA和R2R阵列目前,ROCKNA这个罗马尼亚品牌在Hi-End数字音频界如日中天,它的解码器、数字转盘/服务器在发烧圈中拥有崇高的地位,而更令发烧友感兴趣的是--和其它Hi-End产品相比,ROCKNA的价格并不属于高不可攀的类型。ROCKNA的创始人和设计师Nicolae Jitariu是数码达人,曾是MSB Technology、Goldmund、PS Audio、Wadia、Jadis等多个Hi-End品牌的顾问。Nicolae Jitariu在1999年创立ROCKNA。 展开更多
关键词 Wavedream Reference ROCKNA Hi-End数字音频
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Mango pangenome reveals dramatic impacts of reference bias on population genomic analyses 认领 引用
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作者 Bilal Ahmad Ying Su +25 位作者 Yani Hao Tayyaba Razzaq Rida Arshad Yi Zhang Yingchun Zhang Xingyi Wang Guizhou Huang Xiangnian Su Ting Hou Chaochao Li Xuanwen Yang Chuanning Li Zhenzhou Chu Qiuyan Wang Yu Zhang Zhongxin Jin Qi Xu Xiaodong Xu Yanling Peng Guiqi Bi Chengjie Chen Yeyuan Chen Hua Xiao Jianfeng Huang Yongfeng Zhou Xinmin Tian 《Horticulture Research》 SCIE CSCD 2025年第9期170-182,共13页
Most genomic studies start by mapping sequencing data to a reference genome.The quality of reference genome assembly,genetic relatedness to the studied population,and the mapping method employed directly impact varian... Most genomic studies start by mapping sequencing data to a reference genome.The quality of reference genome assembly,genetic relatedness to the studied population,and the mapping method employed directly impact variant calling accuracy and subsequent genomic analyses,introducing reference bias and resulting in erroneous conclusions.However,the impacts of reference bias have gained limited attention.This study compared population genomic analyses using four different reference genomes of mango(Mangifera indica),including the two haploid assemblies of haplotype-resolved telomere-to-telomere(T2T)genome assembly,a pangenome,and an older version of the reference genome available on NCBI.The choice of reference genome dramatically impacted the mapping efficiency and resulted in notable differences in calling the genetic variants,particularly structural variations(SVs).Phylogenetic analysis was more sensitive to the reference genome compared to genetic differentiation.Population genomic analyses of artificial selection in domestication and SV hotspot regions varied across reference genomes.Notably,the gene enrichment analyses showed significant differences in the top enriched biological processes depending on the reference genome used.Overall,the mango pangenome outperformed the other reference genomes across various metrics,followed by T2T reference genomes,as they captured greater diversity and effectively reduced reference bias.Our findings highlight the role of the mango pangenome in reducing reference bias and underscore the critical role of reference genome selection,suggesting that it is one of the most important factors in population genomic studies. 展开更多
关键词 mapping sequencing data reference genomethe genomic studies reference genome population genomic analyses mapping method genomic analysesintroducing reference bias
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Optimization and identification of stable reference genes for RT-qPCR analysis in Capsicum annuum L. 认领 引用
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作者 Niu Zhan Sile Liu +5 位作者 Yanfang Zhang Ning Yuan Zhisheng Wu Yiting Wang Yi Liu Zhongyi Wang 《Vegetable Research》 2025年第1期12-21,共10页
As a fundamental methodology in gene expression analysis,reverse transcription quantitative PCR(RT-qPCR)requires rigorous normalization using endogenous reference genes demonstrating expression stability across divers... As a fundamental methodology in gene expression analysis,reverse transcription quantitative PCR(RT-qPCR)requires rigorous normalization using endogenous reference genes demonstrating expression stability across diverse experimental conditions.Although RT-qPCR has become ubiquitous in plant biotechnology,the rapid evolution of genomic technologies and burgeoning omics datasets have imposed stringent requirements for reference gene optimization,particularly in non-model organisms like pepper,in which high-quality genome assemblies have only recently been generated.Leveraging the chromosome-level Capsicum annuum'Zunla-1'genome assembly,the study developed a reference gene validation framework that integrates five complementary algorithms or evaluation parameters(GeNorm,NormFinder,BestKeeper,ΔCt,and Cq values).Through systematic evaluation of nine evolutionarily conserved candidate genes(CaGAPDH1/2,CaUBIQUITIN1/2,CaTUBULIN1/2,CaActin1-3)across six tissue types(root,stem,leaf,flower,ovary,and fruit),and five experimental treatment conditions(IAA,GA3,waterlogging,drought,and pathogen),CaGAPDH2 and CaActin1 were validated as optimal reference genes in pepper.Specifically,CaActin1 exhibited exceptional stability under phytohormone treatments,establishing it as the optimal reference gene for hormonal response studies.CaGAPDH2 demonstrated robust performance across multiple stress conditions,validating its utility for stress-related expression profiling. 展开更多
关键词 reference gene optimizationparticularly stable reference genes genomic technologies identification optimization endogenous reference genes demonstrating expression stability omics datasets gene expression analysisreverse transcription quantitative pcr rt qpcr requires
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Age-and gender-specific dynamics and next-generation reference intervals for pepsinogen in northern China 认领 引用
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作者 Miao-Miao Zhang Dong Zhu +1 位作者 Hai-Bin Zhao Xiu-Ying Zhao 《World Journal of Gastroenterology》 SCIE CAS 2025年第31期63-74,共12页
BACKGROUND Pepsinogen(PG)and the PG I/II ratio(PGR)are critical indicators for diagnosing Helicobacter pylori infection and chronic atrophic gastritis,and assessing gastric cancer risk.Existing reference intervals(RIs... BACKGROUND Pepsinogen(PG)and the PG I/II ratio(PGR)are critical indicators for diagnosing Helicobacter pylori infection and chronic atrophic gastritis,and assessing gastric cancer risk.Existing reference intervals(RIs)often overlook age,sex,and demographic variations.Partitioned RIs,while considering these factors,fail to capture the gradual age-related physiological changes.Next-generation RIs offer a solution to this limitation.AIM To investigate age-and sex-specific dynamics of PG and establish next-generation RIs for adults and the elderly in northern China.METHODS After screening,708 healthy individuals were included in this observational study.Serum PG was measured using chemiluminescence immunoassay.Age-and sex-related effects on PG were analyzed with a two-way analysis of variance.RI partitioning was determined by the standard deviation ratio(SDR).Traditional RIs were established using a non-parametric approach.Generalized Additive Models for Location,Scale,and Shape(GAMLSS)modeled age-related trends and continuous reference percentiles for PG I and PG II.Reference limit flagging rates for both RI types were compared.RESULTS PG I and PG II levels were influenced by age(P<0.001)and sex(P<0.001),while PGR remained stable.Age-specific RIs were required for PG I(SDR=0.366)and PG II(SDR=0.424).Partitioned RIs were established for PG I and PG II,with a single RI for PGR.GAMLSS modeling revealed distinct age-dependent trajectories:PG I increased from a median of 39.75μg/L at age 20 years to 49.75μg/L at age 60 years,a 25.16%increase,after which it plateaued through age 80 years.In contrast,PG II showed a continuous rise throughout the age range,with the median value increasing from 5.07μg/L at age 20 years to 8.36μg/L at age 80 years,corresponding to a 64.89%increase.Continuous reference percentiles intuitively reflected these trends and were detailed in this study.Next-generation RIs demonstrated superior accuracy compared to partitioned RIs when applied to specific age subgroups.CONCLUSION This study elucidates the age-and sex-specific dynamics of PG and,to our knowledge,is the first to establish next-generation RIs for PG,supporting more individualized interpretation in laboratory medicine. 展开更多
关键词 Pepsinogen Reference intervals Next-generation reference intervals Age dynamics Gastric biomarkers Personalized medicine
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Systematic identification and validation of the reference genes from 447 transcriptome datasets of moso bamboo(Phyllostachys edulis) 认领 引用 被引量：4
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作者 Yan Liu Chenglei Zhu +4 位作者 Zeming Lin Hui Li Xiaolin Di Xianghua Yue Zhimin Gao 《Horticultural Plant Journal》 SCIE CAS CSCD 2025年第3期1353-1363,共11页
Bamboo was one of the first plants to be cultivated in China and is widely used in industry and daily life.The study of gene function has become an important part of bamboo breeding,whereas quantitative real-time PCR(... Bamboo was one of the first plants to be cultivated in China and is widely used in industry and daily life.The study of gene function has become an important part of bamboo breeding,whereas quantitative real-time PCR(qRT-PCR)is a powerful tool for gene expression analysis.The accuracy of qRT-PCR results largely depends on suitable reference genes.In this study,a transcriptome-wide identification of reference genes was conducted based on 447 transcriptome datasets,comprising 200 tissue samples,107 treated samples,and 140 samples from various moso bamboo(Phyllostachys edulis)forms.A total of 3444,1013,and 3962 stably expressed genes were identified from these three groups,respectively.Functional enrichment analysis revealed significant enrichment of these genes in pathways,including the spliceosome,proteasome,and oxidative phosphorylation.Eight candidate genes(ADPRE,GAPDH,TRX,TUBA,NRP,MBF,UNK,and CAM1),were selected for qRT-PCR validation using 112 samples.To assess their stability,five statistical methods(geNorm,NormFinder,BestKeeper,Delta-Ct,and RefFinder)were employed.The most suitable reference genes were ADPRE and GAPDH for different tissues,GAPDH and CAM1 for different treatments,and GAPDH and TRX for various moso bamboo forms.Overall,ADPRE and GAPDH were the most stable reference genes across all conditions,while TUBA and TRX were the least stable reference genes.In addition,a significant negative correlation was found between the Ct values of RT-qPCR and the log2TPM values from the transcriptome data(Ct=-1.534x+37.221),providing a potential method for estimating gene expression levels.The identified reference genes,particularly ADPRE and GAPDH,provide a robust set of references for gene expression studies in moso bamboo. 展开更多
关键词 Phyllostachys edulis Reference gene qRT-PCR Transcriptome
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Metrological traceable calibration of organic carbon and elemental carbon based on laboratory-generated reference materials 认领 引用 被引量：1
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作者 Yue Liu Junjie Liu +3 位作者 Ji Xiao Jing Ye Lixu Guo Caiqing Yan 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2025年第3期524-534,共11页
Carbonaceous aerosol,including organic carbon(OC)and elemental carbon(EC),has significant influence on human health,air quality and climate change.Accurate measurement of carbonaceous aerosol is essential to reduce th... Carbonaceous aerosol,including organic carbon(OC)and elemental carbon(EC),has significant influence on human health,air quality and climate change.Accurate measurement of carbonaceous aerosol is essential to reduce the uncertainty of radiative forcing estimation and source apportionment.The accurate separation of OC and EC is controversial due to the charring of OC.Therefore,the development of reference materials(RM)for the validation of OC/EC separation is an important basis for further study.Previous RMs were mainly based on ambient air sampling,which could not provide traceability of OC and EC concentration.To develop traceable RMs with known OC/EC contents,our study applied an improved aerosol generation and mixing technique,providing uniform deposition of particles on quartz filters.To generate OC aerosol with similar pyrolytic property of ambient aerosol,both water soluble organic carbon(WSOC)and water insoluble organic carbon(WIOC)were used,and amorphous carbon was selected for EC surrogate.The RMs were analyzed using different protocols.The homogeneity within the filter was validated,reaching below 2%.The long-term stability of RMs has been validated with RSD ranged from 1.7%–3.2%.Good correlationwas observed between nominal concentration of RMswithmeasured concentration by two protocols,while the difference of EC concentration was within 20%.The results indicated that the newly developed RMs were acceptable for the calibration of OC and EC,which could improve the accuracy of carbonaceous aerosol measurement.Moreover,the laboratory-generated EC-RMs could be suitable for the calibration of equivalent BC concentration by Aethalometers. 展开更多
关键词 Carbonaceous aerosol Split point Reference material Laboratory-generated Traceability
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New Potential Barite Reference Materials for LA-MC-ICP-MS Sulfur Isotope Analysis with Application to Hydrothermal Barite in the Huayangchuan Deposit,Western China 认领 引用 被引量：1
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作者 Jiali Fu Xinqian He +4 位作者 Zhaochu Hu Shuo Yin Jian Ma Kaiyun Chen Wen Zhang 《Journal of Earth Science》 SCIE CAS CSCD 2025年第1期1-10,共10页
Sulfur isotopes of S-bearing materials are powerful tools to trace various geological processes and sulfur sources in earth sciences,especially in ore deposits where sulfide-sulfate pair coprecipitates widely.However,... Sulfur isotopes of S-bearing materials are powerful tools to trace various geological processes and sulfur sources in earth sciences,especially in ore deposits where sulfide-sulfate pair coprecipitates widely.However,in-situ S isotope determination of barite is challenging without natural matrix-matched reference material.In this study,we present two natural barite reference materials(1-YS and 294-YS)for in-situ sulfur isotopic analysis.Independent LA-MC-ICP-MS laboratories were utilized to test theδ34S micron-scale homogeneity of 1-YS and 294-YS barites that have 2s repeatabilities of better than±0.45‰and±0.41‰,respectively.Meanwhile,the in-situ analysis results are consistent with the results of the bulk analysis by GS-IRMS within uncertainty.The grand meanδ~(34)S values of 1-YS(13.37‰±0.42‰,2s)and 294-YS(14.38‰±0.44‰,2s)are the final recommended values obtained from four independent laboratories.All the results confirm the suitability of 1-YS and 294-YS barite used as calibration materials with respect to in-situ S isotopic analysis.Moreover,the new developed barite reference materials were used as matrix-matched standard to calibrate the barite samples from the Huayangchuan carbonatite-hosted U-polymetallic deposit(Qinling orogenic belt,western China)to obtainδ34S values.Utilizing the temperaturedependentδ34S fractionation of barite-pyrite pair,we calculate the formation temperature of barite(i.e.,506 to 537°C)and theδ34S value of mineralizing fluid(i.e.,-7.11‰to-7.59‰)in the Huayangchuan deposit.The results indicate an involvement of sedimentary sulfur,presumably acting as a potential uranium source(e.g.,upper crustal materials)for the giant Huayangchuan deposit. 展开更多
关键词 LA-MC-ICP-MS S isotope reference material barite geothermometry
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Recommendations of Stable Mg,Si,V,Fe,Cu,Zn,Rb,Sr,Ag,Cd,Ba,and U Isotope Compositions for Multiple Geological References 认领 引用 被引量：1
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作者 Jinting Kang Xuqi Chen +16 位作者 Xi Deng Yuan Fang Haichuan Jiang Chengyihong Liu Cuihua Luo Xing Li Yuchao Lin Zhaoqi Ren Jiaru Sheng Xue Tang Liyi Xu Jinyi Yan Yaqi Zhang Zhengyu Hou Fei Wu Huimin Yu Fang Huang 《Journal of Earth Science》 SCIE CAS CSCD 2025年第4期1408-1424,共17页
The Metal Stable Isotope Geochemistry Laboratory(MSIGL)at the University of Science and Technology of China has developed state-of-the-art analytical methods for twelve stable isotope systems,including Mg,Si,V,Fe,Cu,Z... The Metal Stable Isotope Geochemistry Laboratory(MSIGL)at the University of Science and Technology of China has developed state-of-the-art analytical methods for twelve stable isotope systems,including Mg,Si,V,Fe,Cu,Zn,Rb,Sr,Ag,Cd,Ba,and U.Geological and biological samples were first digested by acid dissolution or alkali dissolution.The target element was subsequently purified by the column chromatography method.A Neptune Plus MC-ICP-MS was used to measure isotope compositions and the isotope bias caused during measurements was calibrated by standard bracketing and/or the double spike method.The analytical procedure was carefully checked to ensure the high precision and accuracy of the data.Here,we summarized the protocol of these established methods and compiled the standard data measured at our lab as well as those reported in literature.This comprehensive dataset can serve as a reliable benchmark for calibration,method validation,and quality assurance in metal stable isotope analyses. 展开更多
关键词 non-traditional stable isotope metal stable isotope analytical methods MC-ICP-MS standards geological references
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Identification and validation of reference genes for qRT-PCR analyses under different experimental conditions in Dendrobium nobile 认领 引用
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作者 Kaijie Li Xianfen Deng +6 位作者 Yao Wu Jinling Li Mingjin Huang Chunli Luo Hualei Wang Hongchang Liu Dandan Li 《Medicinal Plant Biology》 2025年第1期430-439,共10页
Dendrobium nobile L.(D.nobile),a perennial epiphytic herb belonging to the Orchidaceae family,is widely utilized in traditional Chinese medicine.Identifying stable reference genes(RGs)in D.nobile under various experim... Dendrobium nobile L.(D.nobile),a perennial epiphytic herb belonging to the Orchidaceae family,is widely utilized in traditional Chinese medicine.Identifying stable reference genes(RGs)in D.nobile under various experimental conditions is essential for accurate quantitative real-time polymerase chain reaction(qRT-PCR)analysis.In this study,eight genes were selected as the candidate RGs based on the transcriptome data from different tissues of D.nobile.The qRT-PCR experiments were conducted under diverse experimental conditions,including various tissues and developmental stages,temperature stress,drought stress,and methyl jasmonate(MeJA)stress.The software programs GeNorm,NormFinder,BestKeeper,ΔCt,and RefFinder were employed to evaluate the stability of the candidate RGs.The results indicated that Actin was the most stable reference gene(RG)across different tissues,UBCE2 was the most stable RG under MeJA stress,while ADF7 exhibited the highest stability in various developmental stages,low-temperature stress,and drought stress.The reference gene that demonstrated the most stable expression under high-temperature stress was 60SRPL26.The experiment also validated the selection of optimal reference genes by assessing the expression levels of the farnesyl pyrophosphate synthase gene(FPPS)in different tissues and developmental stages of D.nobile.Furthermore,the expression levels of DnFPPS were analyzed under various stress conditions using qRT-PCR with the most stable reference genes,and the results were consistent with RNA-seq data.These findings provide valuable guidance for future gene expression studies involving qRT-PCR in D.nobile. 展开更多
关键词 reference genes transcriptome data dendrobium nobile stress response transcriptome analysis stable reference genes rgs gene expression quantitative real time polymerase chain reaction
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A chromosome-level reference genome facilitates the discovery of clubroot-resistant gene Crr5 in Chinese cabbage 认领 引用 被引量：5
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作者 Shuangjuan Yang Xiangfeng Wang +8 位作者 Zhaojun Wang Wenjing Zhang Henan Su Xiaochun Wei Yanyan Zhao Zhiyong Wang Xiaowei Zhang Li Guo Yuxiang Yuan 《Horticulture Research》 SCIE CSCD 2025年第3期54-66,共13页
Brassica rapa includes a variety of important vegetable and oilseed crops,yet it is significantly challenged by clubroot disease.Notably,the majority of genotypes of B.rapa with published genomes exhibit high suscepti... Brassica rapa includes a variety of important vegetable and oilseed crops,yet it is significantly challenged by clubroot disease.Notably,the majority of genotypes of B.rapa with published genomes exhibit high susceptibility to clubroot disease.The present study presents a high-quality chromosome-level sequence of the genome of the DH40 clubroot-resistant(CR)line,a doubled haploid line derived from the hybrid progeny of a European turnip(ECD01)and two lines of Chinese cabbage.The assembled genome spans 420.92 Mb,with a contig N50 size of 11.97 Mb.Comparative genomics studies revealed that the DH40 line is more closely related to the Chinese cabbage Chiifu than to the turnip ECD04.The DH40 genome provided direct reference and greatly facilitate the map-based cloning of the clubroot resistance gene Crr5,encoding a nucleotide-binding leucine-rich repeat(NLR)protein.Further functional analysis demonstrated that Crr5 confers clubroot resistance in both Chinese cabbage and transgenic Arabidopsis.It responds to inoculation with Plasmodiophora brassicae and is expressed in both roots and leaves.Subcellular localization shows that Crr5 is present in the nucleus.Notably,the Toll/interleukin-1 receptor(TIR)domain of Crr5 can autoactivate and trigger cell death.In addition,we developed two Crr5-specific Kompetitive allele-specific PCR(KASP)markers and showcased their successful application in breeding CR Chinese cabbage through marker-assisted selection.Overall,our research offers valuable resources for genetic and genomic studies in B.rapa and deepens our understanding of the molecular mechanisms underlying clubroot resistance against P.brassicae. 展开更多
关键词 brassica rapa CRR assembled genome hybrid progeny clubroot resistant gene doubled haploid line chromosome level reference genome map based cloning
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