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急性髓系白血病患者外周血WT1 mRNA表达水平的检测 认领 引用
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作者 聂常富 王建国 +2 位作者 郭志华 荆晓岳 何蕴韶 《中国工业医学杂志》 CAS 北大核心 2004年第6期353-355,共3页
目的 建立realtimeRT PCR检测WT1基因表达的方法 ,了解急性髓系白血病 (AML)患者外周血WT1基因的表达水平。方法 建立realtimeRT PCR方法 ,用PEABI 770 0PCR仪检测 66例AML患者、 2 6例非白血病患者和3 6例正常人外周血中WT1基因的mRN... 目的 建立realtimeRT PCR检测WT1基因表达的方法 ,了解急性髓系白血病 (AML)患者外周血WT1基因的表达水平。方法 建立realtimeRT PCR方法 ,用PEABI 770 0PCR仪检测 66例AML患者、 2 6例非白血病患者和3 6例正常人外周血中WT1基因的mRNA表达水平。结果  2 2例AML初发患者WT1基因的表达量为 (10 5~ 10 6)拷贝 /μgRNA ,取得部分缓解的 2 6例AML患者的表达水平为 (10 2 ~ 10 4)拷贝 /μgRNA ,而取得完全缓解的 17例AML患者的表达水平为 (0~ 10 2 )拷贝 /μgRNA。 结论 WT1基因在AML外周血中有高的表达水平 ,可作为疗效考核及监测微残留病的指标。 展开更多
关键词 急性髓系白血病 Wilm’s瘤基因 实时 逆转录聚合酶链反应
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《荷华文语类参》同音字汇 认领 引用
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作者 连小铃 《闽南师范大学学报(哲学社会科学版)》 2024年第2期46-61,共16页
一百三十年前荷兰汉学家施莱格编撰的《荷华文语类参》是目前刊行版页最多的闽南方言辞典,反映了十九世纪末漳州府龙溪县方言音系。根据《荷华文语类参》记音,对其声母、韵母和声调系统进行归纳与整理,并整理同音字表约八千字。通过与... 一百三十年前荷兰汉学家施莱格编撰的《荷华文语类参》是目前刊行版页最多的闽南方言辞典,反映了十九世纪末漳州府龙溪县方言音系。根据《荷华文语类参》记音,对其声母、韵母和声调系统进行归纳与整理,并整理同音字表约八千字。通过与现代漳州方言的同音字汇进行对比,可以看出一百三十多年来漳州方言的字音结构相对稳定,语言接触带来的新成分也多以层次重叠的方式呈现,现代漳州方言有部分[i?]韵的字已经演变为[i?]韵。 展开更多
关键词 《荷华文语类参》 漳州方言 闽南方言 同音字汇
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A fully automated rotary microfluidic platform for high-throughput multiplex detection of respiratory tract pathogens 认领 引用
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作者 Daoguangyao Zhang Anyi Li +7 位作者 Chuanlong Li Pei Ren Liming Zhang Bin Liu Shiyong Yu Xuefei Lv Wenzhe Si Yulin Deng 《Microsystems & Nanoengineering》 SCIE EI CSCD 2025年第5期489-498,共10页
Rapid,high-throughput,timely,multiplex diagnosis of respiratory-tract infections still relies on laboratory infrastructure,sequential assays,and trained personnel,thereby delaying targeted therapy and outbreak contain... Rapid,high-throughput,timely,multiplex diagnosis of respiratory-tract infections still relies on laboratory infrastructure,sequential assays,and trained personnel,thereby delaying targeted therapy and outbreak containment.In this study,a Fully Automated rotary microfluidic platform(FA-RMP)for high-throughput multiplex respiratory tract pathogens detection was presented.FA-RMP enables a true“sample-in,result-out”workflow through the integration of swab lysis,reagent partitioning,lyophilized reverse transcription loop-mediated isothermal amplification(RT-LAMP),and movingprobe fluorescence read-out,all encapsulated with a disposable microfluidic cartridge and paired with a 9 kg,fourchannel benchtop reader.The FA-RMP enables parallel processing of 16 independent reactions within 30 min,supporting simultaneous detection of up to 4 distinct clinical samples.Analytical validation using serially diluted Mycoplasma pneumoniae(MP)DNA established a limit of detection(LoD)of 50 copies μL-1 and a log-linear correlation between threshold time and template load(R2=0.9528).Testing with eight non-target respiratory pathogens yielded no amplification,confirming high analytical specificity.FA-RMP successfully detected the clinical samples with influenza A,influenza B,and MP,further demonstrating its robust multiplex detection capability.By integrating automated sample preparation,multiplex isothermal amplification and quantitative detection into a portable,high-throughput system,the platform delivers laboratory-grade performance at the point of care,serving as a scalable tool for routine respiratory pathogens screening and rapid epidemic response. 展开更多
关键词 swab lysisreagent partitioninglyophilized reverse transcripti targeted therapy rotary microfluidic platform fa rmp high throughput multiplex detection outbreak containmentin automated rotary microfluidic platform respiratory tract pathogens detection sample result workflow
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