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Development and application of a novel TaqMan qPCR assay targeting the gN gene for genotyping pseudorabies virus 认领 引用
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作者 Yulan JIN Xuan JI +3 位作者 Weiren DONG Yanming HUANG Jiyong ZHOU Jinyan GU 《Journal of Zhejiang University-SCIENCE B》 SCIE CAS CSCD 2026年第8期923-926,I0033-I0034,I0035,共4页
Pseudorabies virus(PRV),known as porcine her-pesvirus type I,belongs to theα-herpesvirus subfamily of the herpesviridae family.Its virions are oval or round,with a genome of approximately 143000 bp and a high guanine... Pseudorabies virus(PRV),known as porcine her-pesvirus type I,belongs to theα-herpesvirus subfamily of the herpesviridae family.Its virions are oval or round,with a genome of approximately 143000 bp and a high guanine-cytosine(GC)content of 73%(Pomeranz et al.,2005). 展开更多
关键词 Alpha herpesvirus Taqman QPCR Gn gene Pseudorabies virus Porcine herpesvirus type I Herpesviridae Virion pseudorabies virus prv known
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Extracting useful information from sparsely logged wellbores for improved rock typing of heterogeneous reservoir characterization using well-log attributes, feature influence and optimization 认领 引用
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作者 David A.Wood 《Petroleum Science》 SCIE EI CAS CSCD 2025年第6期2307-2311,共5页
The information from sparsely logged wellbores is currently under-utilized in reservoir simulation models and their proxies using deep and machine learning (DL/ML).This is particularly problematic for large heterogene... The information from sparsely logged wellbores is currently under-utilized in reservoir simulation models and their proxies using deep and machine learning (DL/ML).This is particularly problematic for large heterogeneous gas/oil reservoirs being considered for repurposing as gas storage reservoirs for CH4,CO2 or H2 and/or enhanced oil recovery technologies.Lack of well-log data leads to inadequate spatial definition of complex models due to the large uncertainties associated with the extrapolation of petrophysical rock types (PRT) calibrated with limited core data across heterogeneous and/or anisotropic reservoirs.Extracting well-log attributes from the few well logs available in many wells and tying PRT predictions based on them to seismic data has the potential to substantially improve the confidence in PRT 3D-mapping across such reservoirs.That process becomes more efficient when coupled with DL/ML models incorporating feature importance and optimized,dual-objective feature selection techniques. 展开更多
关键词 Petrophysical/geomechanical rock typing Log attribute calculations Heterogeneous reservoir characterization Core-well-log-seismic integration Feature selection influences
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Image-Based Rock Typing Using Specific Surface and Iterative Convolution-Thresholding Method 认领 引用
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作者 Chaoyu Liu Zhonghua Qiao +1 位作者 Shuyu Sun Yuzhu Wang 《Communications in Mathematical Research》 CSCD 2025年第4期354-369,共16页
Image-based rock typing(IBRT)is an effective way to understand the pore scale heterogeneity of the reservoir samples.IBRT is aimed at segmenting a rock sample's image into different regions where each region repre... Image-based rock typing(IBRT)is an effective way to understand the pore scale heterogeneity of the reservoir samples.IBRT is aimed at segmenting a rock sample's image into different regions where each region represents a homogeneous porous medium,also known as rock type.Currently,the phase-field rock typing method has attracted more attention due to its impressive performance in classifying the heterogeneous rock images with highly irregular pore structures.In this paper,a modified specific surface CV(SSCV)model is proposed to realize the IBRT.In the SSCV model,the specific surface of a pixel is calculated within a given size neighborhood to distinguish different rock types,and the iterative convolution-thresholding method(ICTM)is applied as the classifier.Compared to the LHFCV method,an existing phase-field rock typing method,the proposed SSCV is capable of processing the images with more than two rock types and can be solved by ICTM which has higher computational efficiency.The proposed SSCV method has demonstrated remarkable performance in the segmentation of various images of both synthetic and natural rock samples. 展开更多
关键词 Rock typing special surface SSCV ICTM
Clinical settings in which human leukocyte antigen typing is still useful in the diagnosis of celiac disease 认领 引用
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作者 Enrico Schirru Rossano Rossino +4 位作者 Rita D Jores Mara Corpino Sandro Muntoni Francesco Cucca Mauro Congia 《World Journal of Gastroenterology》 SCIE CAS 2025年第14期6-17,共12页
Celiac disease(CD)is a systemic autoimmune disorder triggered by gluten ingestion ingenetically predisposed individuals.It is characterized by intestinal histological damage and the production of specific autoantibodi... Celiac disease(CD)is a systemic autoimmune disorder triggered by gluten ingestion ingenetically predisposed individuals.It is characterized by intestinal histological damage and the production of specific autoantibodies.The latest European Society for Paediatric Gastroenterology,Hepatology,and Nutrition(ESPGHAN)2020 guidelines have excluded human leukocyte antigen(HLA)genotyping from the no-biopsy diagnostic approach due to its weak positive predictive value,limited availability,and high cost in some countries.However,HLA genetic testing remains valuable in certain clinical contexts.This study provided practical indications for when to request and how to interpret HLA genotyping,emphasizing its continued relevance for CD diagnosis in specific cases.We also proposed a strategy for monitoring the risk of developing type 1 diabetes(T1D)in patients with CD,based on the risk stratification carried by different HLA genotypes.A retrospective analysis of 746 patients with CD and 627 controls was conducted at our hospital starting in2012,when HLA genotyping became mandatory for the diagnosis of CD.We identified key clinical scenarios where HLA testing remains useful.Several high risk HLA-DQ genotypes strongly associated with CD were highlighted,including HLA-DQ2.5/HLA-DQ2.2and HLA-DQ2.5/HLA-DQ2.5.Notably,while the HLA-DQ2.5/HLA-DQ2.2 genotype is linked to CD,it appears to confer protection against T1D.To support clinical practice,we presented a table clarifying commonly used HLA terminology,and another summarized the main clinical situations in which HLAgenotyping should still be considered.These findings underscore the dual role of HLA testing:Not only can it help rule out CD in selected cases,but it also identifies patients with CD at risk for T1D,guiding personalized monitoring strategies. 展开更多
关键词 Human leukocyte antigens Celiac disease Type 1 diabetes Guidelines Anti tissue transglutaminase type 2
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Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital 认领 引用 被引量：1
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作者 Nor Syaza Syahirah Amat Junaidi Nik Noorul Shakira Mohamed Shakrin +3 位作者 Mohammad Fahmi Daman Huri Ahmad Zakwan Kamarudin Mohd Nasir Mohd Desa Wan Mohd Zin Wan Yunus 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2023年第5期220-231,共12页
Objective:To determine the antibiotic resistance profile(ARP)of Staphylococcus(S.)aureus isolates and molecular typing of the methicillin-resistant S.aureus(MRSA)isolates from Tuanku Mizan Armed Forces Hospital(TMAFH)... Objective:To determine the antibiotic resistance profile(ARP)of Staphylococcus(S.)aureus isolates and molecular typing of the methicillin-resistant S.aureus(MRSA)isolates from Tuanku Mizan Armed Forces Hospital(TMAFH),Kuala Lumpur.Methods:The ARP and presence of the pvl gene were determined for 209 S.aureus isolates from clinical specimens.Of these,123 were methicillin-susceptible S.aureus(MSSA)isolates and 86 were MRSA isolates.All MRSA isolates were characterized using SCCmec typing and spa typing.Descriptive analysis was performed to compare the demographic data with the phenotypic and genotypic variables of the S.aureus isolates.Results:No vancomycin-intermediate and-resistant S.aureus(VISA and VRSA,respectively)were detected among the study isolates.The MSSA isolates showed low resistance rates to all tested antibiotics,were commonly invasive(28/42,66.7%),and mostly harboured pvl(35/42,83.3%).Meanwhile,MRSA isolates showed high resistance to penicillin(86/86,100%),ampicillin(86/86,100%),sulbactam/ampicillin(86/86,100%),cefuroxime(81/86,94.19%),cefoperazone(76/86,88.37%),azithromycin(56/86,65.12%),and erythromycin(54/86,62.79%).The majority of MRSA isolates were of SCCmec type IVh(65/86,75.58%),spa type t032(55/85,63.95%),and grouped into spaCC-t022(66/85,77.65%).The t032 type was found to be associated with resistance traits to azithromycin and erythromycin(P<0.05).We also found several spa types that are typically associated with hospital-,community-,and livestock-associated MRSA co-existing in our MRSA population.Conclusions:This study reflected the consistent absence of VISA and VRSA and corroborated the clonal shifting of MRSA isolates in the Malaysian MRSA isolates. 展开更多
关键词 Staphylococcus aureus Methicillin-resistant Staphylococcus aureus spa typing SCCmec typing Antibiotic resistance
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Identification and Typing of Respiratory Adenoviruses in Guangzhou, Southern China Using a Rapid and Simple Method 认领 引用 被引量：13
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作者 Guiyuan Han Hongling Niu +8 位作者 Suhui Zhao Bing Zhu Changbing Wang Yungang Liu Mingjie Zhang Shu Yang Feitong Liu Chengsong Wan Qiwei Zhang 《Virologica Sinica》 CAS CSCD 2013年第2期103-108,共6页
Human adenoviruses (HAdVs), especially HAdV-B3, -E4 and -B7, are associated with Acute Respiratory Disease in Chinese children, and occasionally in adults. In order to establish and document the profiles of the respir... Human adenoviruses (HAdVs), especially HAdV-B3, -E4 and -B7, are associated with Acute Respiratory Disease in Chinese children, and occasionally in adults. In order to establish and document the profiles of the respiratory adenovirus pathogen among children in Guangzhou, Southern China, a rapid, simple and practical method for identification and typing of respiratory adenoviruses was developed and evaluated. One pair of universal PCR primers was designed according to the conserved region of the hexon gene, which can detect not only HAdV-B3, -E4 and -B7, but also HAdV-B14, -F40 and -F41, with a specific 300bp PCR product. Three pairs of type-specific PCR primers were also designed according to the hypervariable regions of the hexon gene to type HAdV-B3, -E4 and -B7 by three independent PCR reactions, making it easy to optimize the PCR conditions. By using this method, one hundred throat swab specimens collected during Oct 2010 to Dec 2011 and suspected of being positive for adenoviral infection were identified and typed for adenoviruses. Of these samples, fifty-five were adenovirus-positive. The most common HAdV type was HAdV-B3, identified in 92.7% of samples, which is not only consistent with the data reported in 2004-2006, but also consistent with the recent report in Hangzhou, eastern China, indicating that HAdV-B3 has been circulating in Guangzhou, and maybe in eastern China, for many years. The method for the respiratory adenovirus identification and typing we developed is rapid, simple and practical, which has a potential in the real-time surveillance of circulating adenovirus strains and also to provide etiological evidence for the adenovirus-relative disease control and prevention in China. 展开更多
关键词 Molecular epidemiology Identification Typing Southern China
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Molecular Typing of Brucella Suis Collected from 1960s to 2010s in China by MLVA and PFGE 认领 引用 被引量：15
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作者 LI Zhen Jun CUI Bu Yun +9 位作者 CHEN Hai CHEN Jing Diao ZHAO Hong Yan PIAO Dong Ri JIANG Hai ZHANG Li TANG Xu KE Chang Wen YAO zhen TIAN Guo Zhong 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2013年第6期504-508,共5页
Brucellosis is a bacterial anthropozoonosis usually caused by Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. Brucella suis, the causative agent of swine brucellosis, is classified into five b... Brucellosis is a bacterial anthropozoonosis usually caused by Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. Brucella suis, the causative agent of swine brucellosis, is classified into five biovars and preferentially infects different animal hostsIll. In China, brucellosis is a national notifiable communicable disease both in animals and in human. In 2009, 35 816 brucellosis cases were reported. The annual incidence was 2.7 per 100 000 population. 展开更多
关键词 MLVA Molecular Typing of Brucella Suis Collected from 1960s to 2010s in China by MLVA and PFGE 2010
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Pitfalls of restriction enzyme analysis in identifying, characterizing,typing, and naming viral pathogens in the era of whole genome data, as illustrated by HAdV type 55 认领 引用 被引量：2
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作者 Qiwei Zhang Shoaleh Dehghan Donald Seto 《Virologica Sinica》 SCIE CAS CSCD 2016年第5期448-453,共6页
Restriction endonuclease analysis(REA),or restriction fragment length polymorphism(RFLP),was useful for identifying and determining the relatedness and putative identities of microbial strains(Tang et al.,1997)and for... Restriction endonuclease analysis(REA),or restriction fragment length polymorphism(RFLP),was useful for identifying and determining the relatedness and putative identities of microbial strains(Tang et al.,1997)and for characterizing and discriminating large numbers of samples inexpensively in the past。 展开更多
关键词 and naming viral pathogens in the era of whole genome data DLL QS characterizing,typing Pitfalls of restriction enzyme analysis in identifying REA type
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Overview of Molecular Typing Tools for The Characterization of Salmonella enterica in Malaysia 认领 引用 被引量：2
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作者 Soo Tein Ngoi Cindy Shuan Ju Teh +1 位作者 Lay Ching Chai Kwai Lin Thong 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第10期751-764,共14页
Salmonella is a member of the family Enterobacteriaceae. This genus comprises two species, namely Salmonella enterica and Salmonella bongori. Salmonella enterica is further divided into six subspecies, namely enterica... Salmonella is a member of the family Enterobacteriaceae. This genus comprises two species, namely Salmonella enterica and Salmonella bongori. Salmonella enterica is further divided into six subspecies, namely enterica, salamae, arizonae, 展开更多
关键词 Overview of Molecular Typing Tools for The Characterization of Salmonella enterica in Malaysia PFGE MLVA PCR
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RAPID HLA-DRB1 GENERIC TYPING BY PCR-SSP METHOD 认领 引用 被引量：2
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作者 邱长春 赵岩 +3 位作者 陶贞寅 朱席林 徐作军 董怡 《Chinese Medical Sciences Journal》 CAS 1995年第1期34-37,共4页
We report a simple and rapid HLA-DRBI generic typing method, PCRSSP, which is practical and inexpensive. We use 9 sequence-specific primers and 2 group specific Primers to define the HI-ADRB1 specificities DR1,DR2, DR... We report a simple and rapid HLA-DRBI generic typing method, PCRSSP, which is practical and inexpensive. We use 9 sequence-specific primers and 2 group specific Primers to define the HI-ADRB1 specificities DR1,DR2, DR3, DR4,DR5,DR6, DR7, DR8, DR9 and DR10. The HLA DR3, DR5,DR6 and DR8 can be amplified by the two primers of DR3568 and DRB1. The DR6 specificity can be identified by excluding the DR3, DR5 and DR8 when the DR3568 are positive. Any individuals can be typed with some exception: the three pairs of phenotype DR3/DR3 and DR3/DR6, DR5/DRS and DR5/DR6,DR8/DR8 and DR8/DR6 cannot be discriminated from each other. We typed 106 unrelated healthy people from Beijing locations in two weeks. We think this typing method is suitable to replace the error-prone serologic HLA-DR tests in routine clinical practice, including the Prospective typing of cadaveric organ donors. 展开更多
关键词 HLA-DR generic typing PCR-SSP
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Synchrotron infrared spectral regions as signatures for foodborne bacterial typing 认领 引用 被引量：1
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作者 Ya-Di Wang Xue-Ling Li +1 位作者 Jun Hu Jun-Hong Lü 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2019年第2期109-113,共5页
Fourier-transform infrared(FTIR) spectroscopy has emerged as a viable alternative to biochemical and molecular biology techniques for bacterial typing with advantages such as short analysis time, low cost and laborato... Fourier-transform infrared(FTIR) spectroscopy has emerged as a viable alternative to biochemical and molecular biology techniques for bacterial typing with advantages such as short analysis time, low cost and laboratorial simplicity. In this study, synchrotron radiationbased FTIR(SR-FTIR) spectroscopy with higher spectral quality was successfully applied to type 16 foodborne pathogenic bacterial strains. Combined with principal component analysis(PCA) and hierarchical cluster analysis(HCA), we found that the specific spectral region1300-1000 cm-1, which reflects the information of phosphate compounds and polysaccharides, can be used as the signature region to cluster the strains into groups similar with genetic taxonomic method. These findings demonstrated that FTIR spectra combined with HCA have a great potential in quickly typing bacteria depending on their biochemical signatures. 展开更多
关键词 Synchrotron radiation FTIR Spectral signature Bacterial typing PCA HCA
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DNA TYPING SYSTEM FOR HLA-A2 ALLELES BY POLYMERASE CHAIN REACTION WITH SEQUENCE- SPECIFIC PRIMERS 认领 引用 被引量：1
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作者 张庆瑞 翟宁 +1 位作者 耿龙 宋芳吉 《Chinese Medical Sciences Journal》 CAS 2001年第3期161-164,共4页
Objective. To establish a PCR- SSP method for discriminating as many HLA- A* 02 alleles, which could easily be introduced into a routine laboratory. Methods. In this study we typed HLA- A* 02 polymorphisms by a sequen... Objective. To establish a PCR- SSP method for discriminating as many HLA- A* 02 alleles, which could easily be introduced into a routine laboratory. Methods. In this study we typed HLA- A* 02 polymorphisms by a sequence- specific primer (SSP) method, which involved round 1 and round 2 PCR reactions to detect 17 HLA- A* 02 alleles (they are HLA- A* 0201- 0217 alleles) covering exon 2 and exon 3. Results. We have found that DNA sample concentration and purity were the most important variables in determining the quality of the results. For identifying correct band size, the size marker used was important. We noticed that different PCR machines performed differently. By this method, we detected 20 HLA- A* 02 positive genomic DNA samples and found 4 kinds of HLA- A* 02 alleles. They were HLA- A* 0201, 0203, 0206 and 0210. Conclusion. The HLA- A* 02 PCR- SSP method was proven to be a reliable and easily applicable typing method. Our results suggest that the SSP described here provides an optimal HLA- A* 02 typing technique that may be useful in selecting donor- recipient pairs in bone marrow transplantation between unrelated individuals. 展开更多
关键词 DNA typing PCR- SSP HLA- A* 02 alleles
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Antimicrobial resistance and molecular typing of Salmonella in the chicken production chain in Hubei Province, China 认领 引用
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作者 Cuirong Wu Lihui Liu +6 位作者 Yuxin Sui Lulu Huang Yang Chen Haihong Hao Yuanhu Pan Zhenli Liu Guyue Cheng 《Animal Diseases》 CAS 2023年第3期188-201,共14页
Salmonella is a significant foodborne zoonotic pathogen that endangers both human and animal health.The goal of this research is to gain a preliminary understanding of Salmonella contamination and antimicrobial resist... Salmonella is a significant foodborne zoonotic pathogen that endangers both human and animal health.The goal of this research is to gain a preliminary understanding of Salmonella contamination and antimicrobial resistance in the chicken production chain in Hubei Province,China.1149 animal and environmental samples were collected from chicken farms,slaughterhouses,and retail markets in six cities across Hubei Province in China from 2019 to 2020,yielding Salmonella isolation rates of 4.68%(28/598),12.21%(47/385),and 9.64%(16/166),respectively.Seventeen distinct serotypes were detected among 53 non-clonal Salmonella strains,of which Meleagridis(26.42%,14/53)was the dominant serotype.Almost half of the strains(49.06%,26/53)were multi-drug resistant(MDR).Whole-genome sequencing(WGS)showed that 10 resistance genes(tetA,blaTEM,parC,qnrs1,floR,aac(6'-ly,aph(6)-ld,aph(3")-b,aac(6')-laa and sul2)and 7 categories of virulence genes were present in all three links in 22 non-clonal dominant serotype strains.It was shown that Salmonella in the chicken production chain in Hubei Province had a high resist-ance rate to Tetracycline(TET,73.58%),Ofloxacin(OFL,69.81%),Florfenicol(FFC,60.38%)and Ampicillin(AMP,39.62%)which was consistent with the widespread use of these drugs in the husbandry industry in China.Salmonella ST types determined by MLST and serotypes determined by WGS had a one-to-one correlation.Minimum spanning tree analysis revealed that there was cross contamination of Salmonella in farms and slaughterhouses,slaughterhouses and markets,animal samples and environmental samples.This work provides useful information for the prevention and control of contamination and antimicrobial resistance of Salmonella in the chicken production chain,as well as demonstrating the dependable role of WGS in Salmonella molecular typing. 展开更多
关键词 Salmonella Serotype Antimicrobial resistance Multi-locus sequence typing Whole genome sequencing
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Genotyping of amino acid-producing Corynebacterium glutamicum strains based on multi-locus sequence typing(MLST)scheme 认领 引用
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作者 Junjie Yang Yimeng Kong Sheng Yang 《Bioresources and Bioprocessing》 EI 2015年第1期1-5,共5页
Background:Confusing parental information may hinder to dissect mechanisms of amino acid hyper-producing Corynebacterium glutamicum strains.Thus,an efficient method for genotyping of the C.glutamicum is heavily called... Background:Confusing parental information may hinder to dissect mechanisms of amino acid hyper-producing Corynebacterium glutamicum strains.Thus,an efficient method for genotyping of the C.glutamicum is heavily called.Results:Multi-locus sequence typing(MLST)is currently the most popular molecular typing technique.But currently this method is not available for C.glutamicum.In this study,a MLST scheme was established based on sequences of seven housekeeping genes,for genotyping of C.glutamicum.The MLST method performed an efficient discrimination of 17 strains and helps to understand the population structure of this bacterium.Conclusions:This work has expanded the MLST method to C.glutamicum and developed an efficient technique to discriminate strains of uncertain origin. 展开更多
关键词 Corynebacterium glutamicum Multi-locus sequence typing Amino acid producing Corynebacterium crenatum 16S rDNA
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Molecular Typing of Aeromonas hydrophila Isolated from Common Carp in Northeast China 认领 引用
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作者 Li Shao-wu Wang Di +1 位作者 Liu Hong-bai Lu Tong-yan 《Journal of Northeast Agricultural University(English Edition)》 CAS 2013年第1期30-36,共7页
A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic character... A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic characteristics were investigated. All of the isolates were identified as A. hydrophila by traditional biochemical method and yielded a 686-bp DNA fragment of the 16S rDNA gene in the PCR experiments. Collected strains were also evaluated for their susceptibility to 17 different antibiotics. The isolates showed an even trend of the resistance and sensitivity to drugs, highly sensitive to antibiotics, such as Levofloxacin, PolymyxinB, Ofloxacin and resistant to antibiotics, such as Bristopen, Lincomycin, Ampicillin, Teicoplanin. Evaluation of genetic diversity was performed on all isolates by molecular typing with enterobacterial repetitive intergenic consensus （ERIC-PCR） method. The results showed that three different types, i.e. type I, ]I, and type ~I, were found in 59 isolates and type III accounted for a large proportion of 54.84%. There was no dominant difference between the tendency of the isolates of Heilongjiang Province and Jilin Province in these three types, which showed DI 〉 I 〉 I, while the isolates of Liaoning Province showed III 〉 II 〉 I. The percentage of different types in different provinces varied in each other; however, they didn＇t show any obvious regional or cluster-specific branches. In conclusion, the ability to distinguish Aeromonas hydrophila strains from diseased common carp with ERIC-PCR would be useful for epidemiological investigation and population genetic analysis of this pathogen in China. 展开更多
关键词 Aeromonas hydrophila Northeast China ERIC-PCR typing
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EXPERIENCE ON HLA TYPING FOR ALLOGENEIC MARROW TRANSPLANTATION IN 217 MEMBERS OF 30 FAMILIES 认领 引用
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作者 王耀平 朱亚忠 +1 位作者 钱龙华 应大明 《Medical Bulletin of Shanghai Jiaotong University》 1993年第1期56-62,共7页
From April 1978 to Oct 1990, 217 members of 30 families who requiredallogeneic marrow transplantation were examined for HLA typing, with HLA standardantiserum and mixed lymphocyte culture (MLC). The following conclusi... From April 1978 to Oct 1990, 217 members of 30 families who requiredallogeneic marrow transplantation were examined for HLA typing, with HLA standardantiserum and mixed lymphocyte culture (MLC). The following conclusions could bedrawn: (1) when the HLA-A, B, C loci between donor and recipient were identical, theD locus also was generally identical too, but, in some rare cases the HLA-A, B, C wereidentical while the D locus remained different detected by MLC SI which wassignificantly increased; (2) in addition to twin, the sib were the main candidates of donorsin allogeneic marrow transplantation, however, if the phenotype of children was compati-ble to that of their parents with no significant stimulation in MLC, the parents could alsobe considered as a donor; (3) if the HLA-A, B, C typing was identical between fatherand motaher, the parents can also be considered as an eligible donor. Using HLA-A, B,C, DR and D loci identical sibs as donor, 9 of 11 cases (aplastic anemia 9 cases andANLL-M2 2 cases who received allogeneic marrow transplantation) had the evidence ofengraftment. Much attention should be paid to the difference of the minorhistocompatibility antigens, such as sex associated antigen between donor and recipient, be-cause even in 11 cases with HLA-A, B, C, DR and D loci identical sibs as donors formarrow transplantation, 5 cases still had the manifestation of GVHD in various degree,4/5 died of severe GVHD. 展开更多
关键词 typing of histocompatibility bone marrow transplantation mixed lymphocyte culture
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Low resolutation fluorotyping of HLA-DRBl locus 认领 引用
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作者 李贤羿 《外科研究与新技术（中英文）》 2003年第2期113-113,共1页
Objective To investigate and evaluate a novel fluorotyping procedure for HLA-DRB1 locus. Methods On the basis of low resolutation SSP technique, the fluorogenic probe was used to establish the fluorotyping procedure o... Objective To investigate and evaluate a novel fluorotyping procedure for HLA-DRB1 locus. Methods On the basis of low resolutation SSP technique, the fluorogenic probe was used to establish the fluorotyping procedure of HLA-DRB1 which was carried out for 46 samples. Results The fluorotyping has been sucessful for all the 46 samples and was completely coincident to the SSP results. As compared to the serological typing of DR locus,the coincidence rate was 66. 3% (61/92) whereas the coincidence rate of both the 2 specific genes was 43. 5% (20/46). Conclusion Fluorotyping is accurate, sensitve, reproducible, depends less on manual manipulation and eliminates the problems related to contamination. 10 refs. 展开更多
关键词 locus serological coincidence contamination manipulation typing manual
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Characterization of Xanthomonas citri pv.citri from China based on spoligotyping 认领 引用 被引量：2
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作者 Wenting Li Zhenxi Li +3 位作者 Jiaquan Huang Meirong Xu Zheng Zheng Xiaoling Deng 《Horticultural Plant Journal》 SCIE CAS CSCD 2022年第6期727-736,共10页
Xanthomonas citri pv.citri(Xcc),a gram-negative bacterium,is the causal agent of citrus canker,one of the most devastating diseases threatening the citrus industry worldwide.Understanding the diversity and population ... Xanthomonas citri pv.citri(Xcc),a gram-negative bacterium,is the causal agent of citrus canker,one of the most devastating diseases threatening the citrus industry worldwide.Understanding the diversity and population structure of Xcc is a prerequisite for disease epidemiological monitoring and effective disease management.Recent characterization of the clustered regularly interspaced short palindromic repeats(CRISPR)/cas(CRISPR-associated proteins genes)system with a highly variable repeat number among species provides a new molecular typing method for bacterial genetic analysis.In this study,we performed systematic in silico analyses of 28 Xcc genomes and identified a credible CRISPR/cas in Xcc strains.Further analysis of CRISPR polymorphisms(repeat number and spacer types)in 129 Xcc A strains collected from six provinces in China identified 15 types of CRISPR arrays with 25 spacers.Phylogenetic analysis of Xcc strains based on the CRISPR locus produced a more reliable and accurate typing result compared to the commonly used loci.In addition,seven associated cas genes—cas1,cas2,cas3,cas4,cas5,cas7(csd2),and cas8(csd1)—were found located adjacent to the CRISPR array.BLAST results showed>99%similarity of seven cas genes among Xcc strains.Homology analysis of spacer sequences showed that six spacers had possible phage/prophage origin.The characterization of the CRISPR/cas system among Xcc strains provided an updated strain typing method for Xcc diversity analysis and yielded a panoramic view of CRISPR evolution for further studies of Xcc-phage interactions. 展开更多
关键词 Xanthomonas citri pv.citri CRISPR Diversity Spacers Typing method
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Implementation of a Petrographical and Petrophysical Workflow Protocol for Studying the Impact of Heterogeneity on the Rock Typing and Reservoir Quality of Reefal Limestone:A Case Study on the Nullipore Carbonates in the Gulf of Suez 认领 引用 被引量：2
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作者 Mona G.SAFA Bassem S.NABAWY +2 位作者 Ahmed M.K.BASAL Mohammad A.OMRAN Aref LASHIN 《Acta Geologica Sinica(English Edition)》 SCIE EI CAS CSCD 2021年第5期1746-1762,共17页
This study focuses on the heterogeneity of the middle Miocene syn-rift Belayim nullipore(reefal)marine sequences in the Gulf of Suez and its impacts on reservoir quality.The sequences consist of coralline algal reef l... This study focuses on the heterogeneity of the middle Miocene syn-rift Belayim nullipore(reefal)marine sequences in the Gulf of Suez and its impacts on reservoir quality.The sequences consist of coralline algal reef limestones with a highly complex dual-porosity system of primary and secondary porosities of widely varying percentages.To achieve a precise mathematical modeling of these reservoir sequences,a workflow protocol was applied to separate these sequences into a number of hydraulic flow units(HFUs)and reservoir rock types(RRTs).This has been achieved by conducting a conventional core analysis on the nullipore marine sequence.To illustrate the heterogeneity of the nullipore reservoir,the Dykstra-Parsons coefficient(V)has been estimated(V=0.91),indicating an extremely heterogeneous reservoir.A slight to high anisotropy(λk)has been assigned for the studied nullipore sequences.A stratigraphic modified Lorenz plot(SMLP)was applied to define the optimum number of HFUs and barriers/baffles in each of the studied wells.Integrating the permeability-porosity,reservoir quality index-normalized porosity index(RQI-NPI)and the RQI-flow zone indicator(RQIFZI)plots,the discrete rock types(DRT)and the R35 techniques enable the discrimination of the reservoir sequences into 4 RRTs/HFUs.The RRT4 packstone samples are characterized by the best reservoir properties(moderate permeability anisotropy,with a good-to-fair reservoir quality index),whereas the RRT1 mudstone samples have the lowest flow and storage capacities,as well as the tightest reservoir quality. 展开更多
关键词 reservoir heterogeneity nullipore rock typing hydraulic flow units Dykstra-Parsons coefficient stratigraphic modified Lorenz plot(SMLP)
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Mycobacteria Interspersed Repetitive Units-Variable Number of Tandem Repeat, Spoligotyping and Drug Resistance of Isolates from Pulmonary Tuberculosois Patients in Kenya 认领 引用
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作者 Perpetual W. Ndungu Samuel Kariuki +2 位作者 Gunturu Revathi Zipporah Ng’ang’a Stefan Niemann 《Advances in Microbiology》 CAS 2017年第3期205-216,共12页
Background: Molecular typing allows a rapid and precise species differentiation and is essential in investigating the spread of specific genotypes and any relationship with drug resistance. Methodology: To compare the... Background: Molecular typing allows a rapid and precise species differentiation and is essential in investigating the spread of specific genotypes and any relationship with drug resistance. Methodology: To compare the discrimination power of 24-loci Mycobacteria interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) to spoligotyping in determining the circulating genotypes of Mycobacterium tuberculosis in isolates from pulmonary tuberculosis patients in Kenya, a total of 204 isolates were typed. Results: Spoligotyping identified 22 spoligo lineages;while 36(17.6%) isolates were not determined. MIRU-VNTR typing identified 12 genotypes;Kenya H37_Rv_ like, S-like that had never been reported before and which were not identified by spoligotyping were identified. Others were Uganda I and II, LAM, Beijing, TUR, EAI, Delhi/C, S and Haarlem. Only 8 (3.9%) were not defined by MIRU-VNTR. Delhi/CAS, EAI, S, S-like, LAM and Beijing had strains that showed resistance to all the five drugs tested. Two strains of EAI and 2 of S genotypes were resistant to all the five drugs tested. Beijing genotype commonly associated with drug resistance was found to be third in drug resistance (14.7%) after Delhi/CAS (28.9%) and LAM (17.6%) with the highest resistance towards isoniazid and pyrazinamide (3.9% each). MIRU-VNTR typing was more discriminative than spoligotyping;identifying 10 unique H37_Rv-like isolates designated KeniaH37_Rv_like genotype and 14 S-like genotype. Conclusion: MIRU-VNTR typing has not been reported in any other study in Kenya and its higher discrimination can help identify genotypes that cannot be determined by spoligotyping. Association of Beijing genotype drug resistance particularly isoniazid should be of concern since it may result in multidrug resistance in the patients. 展开更多
关键词 Tuberculosis MIRU-VNTR Typing Spoligotyping
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