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Quench dynamics of Anderson-localized interacting exciton-polaritons 认领 引用
1
作者 Zhi-Hao Sun Yong-Jia Chong +4 位作者 Yu Guan Zheng-Ye Wang Meng Chen Lei Chen Xingran Xu 《Chinese Physics B》 SCIE EI CAS CSCD 2026年第5期146-156,共11页
Exciton-polaritons,formed through strong coupling between photons and excitons,exhibit unique characteristics such as an extremely small effective mass and a propensity to form Bose-Einstein condensates.The dynamics o... Exciton-polaritons,formed through strong coupling between photons and excitons,exhibit unique characteristics such as an extremely small effective mass and a propensity to form Bose-Einstein condensates.The dynamics of these condensates are governed by a coupled system of equations that describe the condensate wavefunction and the excitonic reservoir density.Using imaginary-time evolution,the ground state of the condensate in a quasi-periodic potential is determined,revealing a transition from localized to delocalized states as the nonlinearity increases.After quenching the interaction strength,localized condensates with weak nonlinearity propagate without significant distortion,whereas those with strong nonlinearity exhibit breathing-like oscillations.Higher reservoir decay rates promote localization and suppress chaotic behavior,highlighting the interplay between nonlinearity and dissipation in determining the system's dynamical behavior. 展开更多
关键词 exciton-polariton quench dynamics chaos
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Plant Growth-Promoting and Disease-Preventing Mechanisms of Biocontrol Strain A3-1 Against Root Rot of Polygonatum cyrtonema Hua 认领 引用
2
作者 HE Mei PENG Yi-qun +5 位作者 YAO Xuan HUANG Hai ZHU Qian-xia YANG Song CHEN Yong GUO Kai-fa 《Agricultural Science & Technology》 2026年第1期56-64,共9页
This study aimed to explore the green biocontrol methods for root rot of Polygonatum cyrtonema Hua and alleviate the continuous cropping obstacles of P.cyrtonema in Xinhua County,Hunan Province.Four pathogenic fungal ... This study aimed to explore the green biocontrol methods for root rot of Polygonatum cyrtonema Hua and alleviate the continuous cropping obstacles of P.cyrtonema in Xinhua County,Hunan Province.Four pathogenic fungal species isolated from diseased plants were taken as the target pathogens,and Bacillus subtilis A3-1 screened and identified in our laboratory was used as the biocontrol agent.The biocontrol potential of A3-1 against root rot of P.cyrtonema was systematically evaluated through plate confrontation experiments,evaluation of plant growthpromoting and fungal pathogen-inhibiting functions,antifungal activity evaluation of metabolites,and hyphal morphology observation.The results showed that A3-1 had multiple plant growth-promoting functions such as producing ammonia,fixing nitrogen,secreting siderophores,and solubilzing organic and inorganic phosphorus.Its ACC deaminase activity reached 65.04 U/mL,and it could secrete cellulase,protease,and amylase.The plate confrontation experiments showed that the strain exhibited the best inhibitory effect on Colletotrichum spaethianum,while its volatile metabolites had a high inhibition rate of 91.39%on Fusarium solani,while causing morphological distortion of hyphae.However,the sterile filtrate of this strain did not show significant antifungal activity.In summary,strain A3-1 showed dual functions of promoting plant growth and inhibiting fungal pathogens,with its volatile gas as the key antifungal component,demonstrating promising application prospects in biocontrol. 展开更多
关键词 Polygonatum cyrtonema Hua Root rot Biocontrol Continuous cropping obstacles Plant growth-promoting function
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Physiological Response Mechanisms of Rice to Cadmium,Manganese,and Their Combined Stress 认领 引用
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作者 Zhenqing LIU Dongli LIU +1 位作者 Chenzhong JIN Jing ZHOU 《Agricultural Biotechnology》 2026年第2期8-16,共9页
[Objectives]This study was conducted to comprehensively elucidate the antioxidant response mechanisms of rice to cadmium(Cd)and manganese(Mn)stress and the interactive effects of their combined stress.[Methods]This st... [Objectives]This study was conducted to comprehensively elucidate the antioxidant response mechanisms of rice to cadmium(Cd)and manganese(Mn)stress and the interactive effects of their combined stress.[Methods]This study systematically investigated the effects of individual and combined Cd and Mn stress on oxidative damage indicators,activities of major antioxidant enzymes(SOD,POD,CAT,APX),expression of key antioxidant and detoxification genes(e.g.,SOD,POD,CAT,GSH1,GST),and contents of osmoregulatory substances(soluble sugars,soluble proteins,proline)in rice leaves.[Results](1)Under single or combined stress of Cd and Mn,the accumulation of reactive oxygen species(ROS)in rice leaves exhibited significant dose-dependence and synergistic effects.(2)Under the Mn500 treatment,the activities of SOD and CAT increased by 7.0%and 19.8%,respectively.Under the Cd5 stress treatment,the activities of POD and APX increased by 22.1%and 55.0%,respectively.The combined stress with low-concentration Mn and Cd significantly increased the activities of SOD and CAT in rice leaves,rising by 18.9%and 45.1%,respectively,compared with single Cd stress.The expression levels of genes encoding CAT and SOD were also significantly upregulated.The combined stress with low-concentration Mn and Cd(Mn500+Cd)significantly upregulated the expression level of glutathione S-transferase(GST).Conversely,under high-concentration Mn and Cd combined stress(Mn3000+Cd20),the expression of glutathione synthesis-related genes was significantly inhibited.(3)Under Cd5 stress,the contents of soluble sugars,soluble proteins,and proline in rice leaves increased by 14.2%,11.5%,and 90.0%,respectively.However,when stress intensity increased(e.g.,under Cd20),leaf soluble protein content decreased significantly.Therefore,it was concluded that high-concentration single stress of either Cd or Mn induced massive accumulation of reactive oxygen species(H 2O 2)and membrane lipid peroxidation(elevated MDA),thereby damaging cell membrane integrity.Under combined stress,high-concentration Mn and Cd synergistically exacerbated oxidative damage,with H 2O 2 and MDA accumulation levels significantly higher than those under individual treatments.The response of the antioxidant system exhibited concentration-dependent characteristics.Specifically,low-concentration Mn alleviated Cd-induced oxidative stress by enhancing the activities of SOD and CAT.In contrast,the combined stress with high-concentration Mn and Cd inhibited the activities of antioxidant enzymes and the expression of their encoding genes(such as SOD,CAT,and the glutathione synthase GSH1),significantly compromising cellular defense capacity.[Conclusions]This study provides a reference for understanding the physiological responses and coping mechanisms of rice to combined stress from other heavy metals. 展开更多
关键词 Rice Cadmium Manganese Stress induction
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Cloning and Expression Vector Construction of BjMADS23 Promoter in Brassica juncea 认领 引用
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作者 JIANG Xiang-yun YUAN Yu-hui +6 位作者 XIE Chun-feng HUANG Jing-yi SUN Zi-yu ZHONG Pin-tian HUANG Wen-jin WANG Ying LIU Xian-jun 《Agricultural Science & Technology》 2026年第1期25-32,共8页
Cloning and analyzing the promoter of BjMADS23 is of great significance for understanding its involvement in regulating stress resistance in Brassica juncea.The promoter sequence of BjMADS23 was cloned via PCR,and cis... Cloning and analyzing the promoter of BjMADS23 is of great significance for understanding its involvement in regulating stress resistance in Brassica juncea.The promoter sequence of BjMADS23 was cloned via PCR,and cis-acting regulatory elements within this promoter were analyzed through the online tool PlantCARE.A plant expression vector pBjMADS23+GUS with fusion of the GUS gene was constructed by seamless cloning.The results showed that the cloned promoter sequence of BjMADS23 was 2450 bp in length,harboring multiple cis-acting elements responsive to light,abscisic acid induction,anaerobic induction,salicylic acid induction,and drought induction.The plant expression vector pBjMADS23+GUS was successfully constructed,providing a foundation for subsequent studies on the transcriptional regulation of BjMADS23 in B.juncea. 展开更多
关键词 Brassica juncea BjMADS23 Promoter Expression vector construction
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Occurrence Investigation and Molecular Biological Identification of Vegetable Thrips in Central Hunan 认领 引用
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作者 Huaibo ZHOU Huixin ZHENG Jun GUO 《Agricultural Biotechnology》 2025年第3期33-35,39,共3页
[Objectives]This study was conducted to better understand the occurrence status of thrips in Central Hunan,clarify thrips species,and provide a basis for scientific control.[Methods]The occurrence of thrips in the Cen... [Objectives]This study was conducted to better understand the occurrence status of thrips in Central Hunan,clarify thrips species,and provide a basis for scientific control.[Methods]The occurrence of thrips in the Central Hunan Region were investigated and analyzed.Through field collection,molecular biological identification and sequencing,thrips populations in the region were identified.[Results]Six species of thrips infesting vegetable crops were identified in the Central Hunan Region,with Megalurothrips usitatus,Thrips palmi,and Frankliniella intonsa being the dominant species.These pests were particularly severe on leguminous and cucurbit crops.[Conclusions]Combined with integrated control strategies,this study provides theoretical and technical support for the scientific management of thrips in the Central Hunan Region,ensuring the sustainable development of the local vegetable industry. 展开更多
关键词 Central Hunan Thrips Species Identification
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Biogeochemical mechanisms of zero-valent iron and biochar for synergistically mitigating antimony uptake in rice 认领 引用 被引量：2
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作者 Xiaofeng Zhang Jialin Chi +7 位作者 Huanyun Yu Liping Fang Tongxu Liu Yanhong Du Chuanping Liu Xiangqin Wang Qian Xu Fangbai Li 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2025年第7期76-86,共11页
Antimony(Sb)contamination in paddy fields can lead to its accumulation in rice grains,posing a threat to food safety.To address this issue,the combined use of zero-valent iron(ZVI)and biochar(BC)were applied to decrea... Antimony(Sb)contamination in paddy fields can lead to its accumulation in rice grains,posing a threat to food safety.To address this issue,the combined use of zero-valent iron(ZVI)and biochar(BC)were applied to decrease the uptake of Sb in Sb-polluted soils,and their effects on Sb uptake from soil to rice grains were investigated.Our results showed that the combination treatment of 0.05%ZVI and 0.095%BC resulted in a significant decrease(42.8%)in Sb accumulation in rice grains that was comparably more efficient than that by 0.05%ZVI(decrease of 15.8%Sb accumulation)or 0.095%BC(decrease of 12.7%Sb accumulation)alone,demonstrating the synergistic effect of ZVI and BC on mitigating Sb uptake by rice plants.ZVI presence resulted in the formation of iron oxides in the soil and on root surfaces,and the S2-/S22-ascent also increased by 58.7%on day 75 compared with that of the control,facilitating the reduction of Sb(Ⅴ)to less mobile Sb(Ⅲ),thereby decreasing Sb accumulation in rice plants.BC initially increased themobility of Sb owing to its alkaline nature,whereas the electron shuttle properties of BC contributed to a decrease in Sbmobility.The abundance of the arsenite-reducing gene arrA ultimately increased by 203.2% on day 120 compared with the initial phase on day 5,and BC caused a remarkable increase in arrA gene abundance.This study revealed the synergistic mechanisms by combining ZVI and BC to mitigate Sb uptake by rice,which may be useful for the sustainable remediation of contaminated rice paddies. 展开更多
关键词 Antimony Zero-valent iron Biochar Synergistic remediation Soil-rice system
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Occurrence and Control of Weeds in Abandoned Fields in Central Hunan 认领 引用
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作者 Yunyun ZHOU Shunli XIAO +4 位作者 Chenzhong JIN Xiu LIU Jun PAN Kuojie XIAO Qiuyuan DUAN 《Agricultural Biotechnology》 2021年第2期31-33,36,共3页
[Objectives]This study was conducted to clarify the occurrence of weeds,and give relevant comprehensive control suggestions accordingly.[Methods]The species and community characteristics of weeds in abandoned rice fie... [Objectives]This study was conducted to clarify the occurrence of weeds,and give relevant comprehensive control suggestions accordingly.[Methods]The species and community characteristics of weeds in abandoned rice fields in central Hunan were investigated and analyzed.[Results]There were 75 species of weeds in the abandoned farmland in central Hunan,belonging to 66 genera,27 families.Among them,22 species had a relative abundance of more than 5%.According to the different water environments of abandoned fields,there were differences in the types and structures of weeds.They succeeded to wetland vegetation types and dryland vegetation types respectively.Species grown in the original farmland still exist in abandoned fields,but they were mainly invaded by field weeds,such as Digitaria sanguinalis,Eleusine indica,Panicum acroanthum,Bidens pilosa,and Xanthium sibiricum.[Conclusions]This study lays a foundation for fully guaranteeing the actual results of re-cultivation of abandoned land in rural areas. 展开更多
关键词 Central Hunan Abandoned fields Weeds Integrated control
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Differential Expression of ATP6V1D and Its Diagnostic Potential in IgA Nephropathy 认领 引用
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作者 Liang Peng Lin Hu +1 位作者 Yi-qun Peng Dong-guang Wang 《Current Medical Science》 SCIE CAS CSCD 2025年第5期1172-1181,共10页
Objective IgA nephropathy(IgAN)is the most prevalent form of primary glomerular disease.However,its diagnosis is contingent on kidney biopsy.Therefore,noninvasive biomarkers are urgently needed for diagnosis.This stud... Objective IgA nephropathy(IgAN)is the most prevalent form of primary glomerular disease.However,its diagnosis is contingent on kidney biopsy.Therefore,noninvasive biomarkers are urgently needed for diagnosis.This study aims to identify novel urinary biomarkers that differentiate IgAN from other common primary glomerular diseases,specifically membranous nephropathy(MN)and minimal change disease(MCD).Methods The peripheral blood mononuclear cell(PBMC)transcriptome dataset GSE73953 was obtained from the GEO database.Differential gene expression,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment,Gene Ontology(GO)enrichment,and immune infiltration analyses were performed.Protein–protein interaction(PPI)analysis and lysosome-related genes were used to identify hub genes.The expression of the hub gene ATP6V1D in urine and kidney tissues from individuals with IgAN,healthy controls,MCD and MN patients was assessed using enzyme-linked immunosorbent assay(ELISA),Western blotting,and immunostaining techniques.Spearman’s correlation analysis was employed to investigate the relationships between the concentration of ATP6V1D in urine,the concentration of galactose-deficient IgA1(GD-IgA1),and the clinical data of patients.The receiver operating characteristic(ROC)curve was used to assess the role of urine ATP6V1D levels in distinguishing IgAN from MN and MCD.Results ATPase was identified as the principal intracellular structure associated with differentially expressed genes(DEGs)between IgAN patients and healthy controls in PBMCs.ATP6V1D was identified as a hub gene at the intersection of lysosome-related and differential genes.ATP6V1D levels were lower in PBMCs,urine,and kidney samples from IgAN patients than in those from healthy individuals,MCD and MN patients.The decreased urinary ATP6V1D levels and increased GD-IgA1 levels in IgAN patients were further validated.These changes were positively correlated with 24-h urine protein levels.Notably,a negative correlation was observed between ATP6V1D and GD-IgA1 levels.ROC curve analysis demonstrated that urinary ATP6V1D(AUC=0.972)and GD-IgA1(AUC=0.952)had significant discriminative power in distinguishing IgAN patients from MCD and MN patients,with no significant difference in predictive performance between the two biomarkers(P>0.05).Conclusions The findings underscore the potential utility of the urine ATP6V1D concentration as a biomarker to distinguish IgAN from MN and MCD. 展开更多
关键词 IgA nephropathy Peripheral blood mononuclear cells Membranous nephropathy Minimal change disease Biomarker
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Effects of Continuous Cropping of Polygonatum odoratum on Microbial Functional Diversity and Selected Environmental Factors in the Rhizosphere Soil 认领 引用
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作者 Manjiang YU Yihong HU +3 位作者 Fan XIE Xiongmei ZHU Chenzhong JIN Jing YE 《Agricultural Biotechnology》 2025年第5期12-18,21,共7页
[Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-cr... [Objectives]This study was conducted to investigate the mechanism of continuous cropping obstacles in Polygonatum odoratum.[Methods]Three treatments were established:continuous cropping(two consecutive crops),first-crop control(with Phaseolus vulgaris as the preceding crop),and blank control.The effects of continuous cropping on the functional diversity of soil microorganisms,soil enzyme activities,and soil nutrient coordination in the rhizosphere soil of P.odoratum during different growth stages were investigated.[Results](1)Continuous cropping increased the carbon source metabolic capacity,Shannon diversity index,and richness of rhizosphere soil microorganisms by 3.2%-14.7%,0.9%-3.5%,and 1.3%-12.5%,respectively,but the differences were not significant.(2)Principal component analysis indicated that during the middle stage of rhizome expansion,continuous cropping significantly altered the characteristics of microbial carbon metabolism,and the microbial communities utilizing carbohydrates,amino acids,polymers,carboxylic acids and amines as carbon sources exhibited vigorous metabolism.(3)Continuous cropping significantly reduced the activities of urease,polyphenol oxidase,and acid phosphatase in rhizosphere soil,with decreases of 24.4%-39.5%,3.2%-14.8%,and 7.9%-18.2%,respectively.The activities of catalase and invertase sometimes exceeded and sometimes fell below those of the first crop,showing no consistent pattern.(4)Under continuous cropping conditions,nutrient imbalance occurred in the rhizosphere soil,characterized by nitrogen deficiency and phosphorus surplus.(5)Grey correlation analysis indicated that available phosphorus content,alkali-hydrolyzable nitrogen content and polyphenol oxidase activity in rhizosphere soil were the main factors influencing microbial functional diversity.[Conclusions]This study provides a theoretical basis for understanding the formation mechanism of continuous cropping obstacles in P.odoratum. 展开更多
关键词 Polygonatum odoratum Continuous cropping Rhizosphere soil Microbial function
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Joint Toxicity of Fluxapyroxad and Pyraclostrobin on Zebrafish and Their Mitochondrial Targeting Mechanisms 认领 引用
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作者 Dongli LIU Xuanqing KONG +3 位作者 Xiaoming OU Zhenqing LIU WenjingYANG Jianhong LIU 《Agricultural Biotechnology》 2025年第6期46-52,共7页
[Objectives]This study was conducted to investigate the joint toxicity of fungicides on aquatic ecosystems.[Methods]Using zebrafish as a model organism,an LC-MS/MS simultaneous detection method was established for flu... [Objectives]This study was conducted to investigate the joint toxicity of fungicides on aquatic ecosystems.[Methods]Using zebrafish as a model organism,an LC-MS/MS simultaneous detection method was established for fluxapyroxad and pyraclostrobin(with detection limits at ng/L level),and their acute toxicity,joint toxicity and toxic mechanisms were systematically evaluated.[Results]The toxicity of pyraclostrobin(96 h-LC 50=0.052 mg/L)to zebrafish was approximately 25.8 times higher than that of fluxapyroxad(96 h-LC 50=1.34 mg/L).Joint toxicity evaluation using the fixed-ratio ray design revealed that six of the seven mixture ratios exhibited additive effects(AI=0.62-1.47),while the 8:1 ratio showed antagonism(AI=2.14).The analysis of toxicity mechanisms indicated that both fungicides induced oxidative stress,lipid peroxidation,and cellular damage through inhibition of mitochondrial complex III and II,respectively,with pyraclostrobin inducing more pronounced hepatic MDA elevation(2.56-fold)and antioxidant enzyme inhibition.Ecological risk assessment demonstrated that fluxapyroxad posed moderate risk(RQ=0.16-0.90),while pyraclostrobin posed moderate to high risk(RQ=0.56-3.56),and crustaceans faced the highest risk.[Conclusions]This study elucidated the mechanism underlying toxicity differences due to distinct mitochondrial targets,providing a scientific basis for fungicide management. 展开更多
关键词 Fluxapyroxad Pyraclostrobin Zebrafish Joint toxicity Mitochondrion Oxidative stress
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Promoter Expression Vector Construction and Expression Profiling of BjB01.MTP5 in Brassica juncea 认领 引用
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作者 SHENG Jia-lin GONG Sha +3 位作者 ZHONG Pin-tian CHEN Ming-zhe YUAN Yu-hui YOU Liang 《Agricultural Science & Technology》 2025年第4期39-45,80,共7页
In this study,we successfully constructed the promoter expression vector ProBjB01.MTP5-GUS for BjB01.MTP5 in Brassica juncea.Phylogenetic and cis-acting element analyses were performed based on the promoter sequenc... In this study,we successfully constructed the promoter expression vector ProBjB01.MTP5-GUS for BjB01.MTP5 in Brassica juncea.Phylogenetic and cis-acting element analyses were performed based on the promoter sequences of the MTP5 gene from ten Brassicaceae species.The results revealed that the MTP5 promoters of the B subgenome in Brassica formed distinct evolutionary branches compared with those of the A/C subgenomes.Analysis of cis-acting elements indicated that elements responsive to phytohormones(e.g.,abscisic acid,methyl jasmonate,and auxin)and abiotic stresses including anaerobic induction,salicylic acid,drought,and low temperatures were highly conserved in the MTP5 promoters across different species.Tissue-specific expression profiling demonstrated that BjB01.MTP5 was predominantly expressed in roots,flower buds,siliques,and pericarps.Furthermore,qRT-PCR analysis under heavy metal stress showed that the expression of BjB01.MTP5 was significantly induced by various heavy metals,such as Mn2+,Fe2+,Zn2+,and Sb3+.Its expression pattern correlated strongly with the distribution of heavy metal-responsive elements in the promoter region.These findings provided important theoretical insights into the biological functions of MTP genes in response to heavy metal stress. 展开更多
关键词 Brassica juncea BjB01.MTP5 Promoter analysis Heavy metal response
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Tissue Expression Pattern and Bioinformatics Analysis of OsKMP2 Gene in Rice 认领 引用
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作者 Jiaqi DING Ziang YI +2 位作者 Qi QIU Chenzhong JIN Taoli LIU 《Agricultural Biotechnology》 2025年第3期1-5,共5页
Kinesins are a superfamily of proteins widely present in eukaryotes,playing crucial roles in plant cell wall assembly,cell elongation regulation,gravity sensing,and fertility control.In this study,bioinformatics analy... Kinesins are a superfamily of proteins widely present in eukaryotes,playing crucial roles in plant cell wall assembly,cell elongation regulation,gravity sensing,and fertility control.In this study,bioinformatics analysis of the OsKMP2 gene(LOC_Os02g28850)was performed using online tools such as ExPASy-ProtParam,ProtScale,CD-search,and DNAMAN software.Additionally,qRT-PCR was employed to analyze the tissue expression pattern of OsKMP2.The results showed that the molecular weight of the OsKMP2 is 118.39728 kDa,and it is a hydrophilic and unstable acidic protein.Secondary structure prediction revealed that it primarily consists ofα-helices(69.45%),random coils(25.19%),and extended strands(5.36%).The gene was expressed in various rice tissues,with the highest expression level observed in leaves.These results indicate that the OsKMP2 gene exhibits high evolutionary conservation and functional diversity in rice. 展开更多
关键词 Rice OsKMP Tissue expression pattern Bioinformatics analysis
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Effect of Different Addition Amounts of Phellodendri Chinensis Cortex Residue and Inoculation of the Microbial Agent ZG2-3 on the Quality of Organic Fertilizer 认领 引用
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作者 HUANG Hai HE Mei +4 位作者 LIAO Kai ZHU Qian-xia LIU Hong-yan JING Yuan-rong GUO Kai-fa 《Agricultural Science & Technology》 2025年第3期31-37,共7页
To determine the addition amount of Phellodendri Chinensis Cortex residue for composting,this study designed four Phellodendri Chinensis Cortex residue treatments of 100(A1),150(A2),200(A3),and 250(A4).Then,mushroom s... To determine the addition amount of Phellodendri Chinensis Cortex residue for composting,this study designed four Phellodendri Chinensis Cortex residue treatments of 100(A1),150(A2),200(A3),and 250(A4).Then,mushroom substrate residue,rice husks,and pig manure were added,and the cellulose-degrading strain ZG2-3 was inoculated at 5%for composting.The inoculation of a commercially available microbial agent was taken as the positive control(PC),and no inoculation of microbial agent as the blank control(CK).The effect of different treatments on the heavy metal content,nutrients,physicochemical properties,and microbial counts of compost and the seed germination index(GI)were studied and compared.The results showed that except that in CK,the content of heavy metals in other treatments did not exceed the standard limits.With the increase in the addition amount of Phellodendri Chinensis Cortex residue,the content of available nitrogen,available phosphorus,available potassium,and total nutrients in the compost also increased.Adding microbial agent significantly increased the content of total nutrients,available nitrogen,available phosphorus,available potassium,total nitrogen,total phosphorus,and total potassium in the compost,while reducing the content of organic matter.The GI was the highest in A4 treatment,reaching 85.33%,and it followed a trend of A3>PC>CK.This indicated that the inoculation of microbial agent accelerated the maturation of compost,and ZG2-3 outperformed the commercially available Bacillus subtilis.Overall,the A4 treatment(250 g Phellodendri Chinensis Cortex residue+125 g mushroom substrate residue+125 g rice husks+1000 g pig manure+inoculation of 5%ZG2-3)performed well in terms of nutrients content,physicochemical properties,and GI and had good safety,being the optimal formula for composting. 展开更多
关键词 Phellodendri Chinensis Cortex residue Composting Heavy metal Organic matter
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Cloning and Expression Vector Construction of BnOPR1 from Brassica napus 认领 引用
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作者 CHENG Yi-yuan YUAN Yu-hui +5 位作者 WANG Yan-hong GONG Sha LING Hui HUANG Jing-yi ZHONG Pin-tian LIU Xian-jun 《Agricultural Science & Technology》 2025年第3期6-14,共9页
The 12-oxophytodienoate reductase(OPR),a flavin mononucleotide-dependent oxidoreductase,regulates plant responses to stress conditions such as heavy metals,drought,saline-alkali,pests,and diseases by participating in ... The 12-oxophytodienoate reductase(OPR),a flavin mononucleotide-dependent oxidoreductase,regulates plant responses to stress conditions such as heavy metals,drought,saline-alkali,pests,and diseases by participating in the synthesis of plant hormones such as jasmonic acid(JA).In this study,homologous cloning was employed to obtain the Brassica napus‘Zhongshuang 11’OPR1 gene(BnOPR1).Bioinformatics analysis was conducted for the deduced protein BnOPR1,and a plant expression vector for BnOPR1 was constructed.The bioinformatics analysis revealed that BnOPR1 was 1125 bp in length,encoding 375 amino acid residues.The deduced protein had a molecular weight of 41.604 kDa,an isoelectric point of 6.05,a molecular formula of C1853H2855N511O550S16,an aliphatic index of 35.47,a lipophilicity index of 74.87,and an instability index of 39.49.It had 49 phosphorylation sites and lacked transmembrane domains and signal peptides.The phylogenetic analysis indicated that BnOPR1 had a close relationship with the OPR1 protein from Brassica rapa since they shared the same clade,while it had a distant relationship with the OPR1 protein from Raphanus sativus.In this paper,the expression vector for BnOPR1 was successfully constructed by seamless cloning and named pBnOPR1.The findings laid a foundation for further studying the roles of BnOPR1 in the response to antimony stress. 展开更多
关键词 Brassica napus BnOPR1 gene Homologous cloning Vector construction
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Cloning and Expression Vector Construction of BnZAT12 in Brassica napus 认领 引用
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作者 HUANG Xin ZHOU Li-hua +3 位作者 YUAN Yu-hui ZHONG Kai CHEN Dong-long LIU Xian-jun 《Agricultural Science & Technology》 2025年第1期45-54,共10页
To investigate the function of the zinc finger protein BnZAT12 in Brassica napus,bioinformatics analysis was conducted on BnZAT12.The results showed that the open reading frame of BnZAT12 was 477 bp in length,encoding... To investigate the function of the zinc finger protein BnZAT12 in Brassica napus,bioinformatics analysis was conducted on BnZAT12.The results showed that the open reading frame of BnZAT12 was 477 bp in length,encoding 158 amino acid residues.The deduced protein had a molecular weight of 16864.72 Da and a theoretical isoelectric point of 9.68.The phylogenetic tree showed that Brassica napus had the closest relationship with Brassica oleracea belonging to Brassicaceae and the farthest relationship with Oryza sativa.The analysis of the promoter region suggested that BnZAT12 may be regulated by factors such as light,abscisic acid,and methyl jasmonate.Furthermore,the BnZAT12 overexpression vector was constructed by seamless cloning.This study laid a foundation of molecular biology for further elucidating the role of BnZAT12. 展开更多
关键词 BnZAT12 Brassica napus Bioinformatics analysis Vector construction
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Trends and patterns in agriculture tourism research:A 30-year bibliometric analysis based on Web of Science 认领 引用
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作者 XIE Ai-liang ZHOU Fan-ming 《Ecological Economy》 2020年第2期120-132,共13页
The article uses the information visualization software Citespace5.5 to study the data related to agriculture tourism in the Web of Science database,from macro to micro to the representative countries of the literatur... The article uses the information visualization software Citespace5.5 to study the data related to agriculture tourism in the Web of Science database,from macro to micro to the representative countries of the literature,keywords and co-cited literatures.Through visualization analysis,the article clarifies the key research directions,key literatures and hot spot frontiers in the field of agriculture tourism research,forecasts the future development trends in this field.The results show that the study of agriculture tourism research has become one of the hot topics in academia.The research results of international agriculture tourism are rich.Scholars have many research perspectives.International agriculture tourism research has formed a mature and stable academic community.Mainstream journals in related international fields play an important role in disseminating agriculture tourism research content,methods,and results.Research stage is progressive.In the future,we can focus on the basic theoretical construction of agriculture tourism research,the deep integration of agriculture and tourism,the regional effects of agriculture tourism,agriculture tourism research methods,and multidisciplinary and multi-regional comparative research,etc.This paper can help readers to better understand the status quo and development trend of agriculture tourism and to easier recognize the shortcomings in the development of agriculture tourism,so as to provide a promising direction for future research. 展开更多
关键词 agriculture tourism WoS mapping knowledge domains Citespace 5.6
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Study on the Optimization of Extraction Technology of Rose laevigate Michx. Polysaccharides and Its in vitro Antioxidant Activity 认领 引用
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作者 Guochen HU Han LIU +5 位作者 Qianqian TANG Li CHENG Chenzhong JIN Yunyun ZHOU Xuejiao ZHANG Guiying LIU 《Agricultural Biotechnology》 2021年第6期68-72,共5页
[Objectives]This study was conducted to optimize the optimal process conditions for extracting polysaccharides from Rose laevigate Michx.with hot water.[Methods]The extraction rate of R.laevigate was used as an evalua... [Objectives]This study was conducted to optimize the optimal process conditions for extracting polysaccharides from Rose laevigate Michx.with hot water.[Methods]The extraction rate of R.laevigate was used as an evaluation index,and extraction temperature,material-to-liquid ratio,extraction time and extraction times were selected as influencing factors.On the basis of single factor tests,L 9(34)orthogonal tests were carried out for optimization,and the content of polysaccharides was determined by the phenol-sulfuric acid method.The in vitro antioxidant activity of the extracted R.laevigate polysaccharides was studied.[Results]The effects of various influencing factors on the extraction rate of R.laevigate polysaccharides ranked as material-liquid ratio>extraction temperature>extraction times>extraction time.The optimal extraction process was extraction temperature 80℃,extraction time 2 h,and material-to-liquid ratio 1∶25,extraction times 3 times,under which the extraction rate was(9.55±0.12)%.R.laevigate polysaccharides had certain scavenging capacities of DPPH free radicals and hydroxyl free radicals,and its scavenging capacity of DPPH free radicals was better than that of hydroxyl free radicals.[Conclusions]This study provides a theoretical basis for the optimization of the extraction process of R.laevigate polysaccharides and the in-depth development and application of polysaccharides. 展开更多
关键词 Rose laevigate Michx. Polysaccharides Extraction Antioxidation
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Poaceae Orthologs of Rice OsSGL,DUF1645 Domain-Containing Genes,Positively Regulate Drought Tolerance,Grain Length and Weight in Rice 认领 引用 被引量：4
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作者 LIU Kai LI Minjuan +4 位作者 ZHANG Bin YIN Xuming XIA Xinjie WANG Manling CUI Yanchun 《Rice science》 SCIE CSCD 2022年第3期257-267,共11页
Grain yield is a polygenic trait that can be influenced by environmental factors and genetic compositions at all plant growth stages.Currently,the molecular mechanisms behind the coordination of the interaction betwee... Grain yield is a polygenic trait that can be influenced by environmental factors and genetic compositions at all plant growth stages.Currently,the molecular mechanisms behind the coordination of the interaction between grain yield-related traits remain unknown.In this study,we characterized the function of four STRESS_tolerance and GRAIN_LENGTH(Os SGL)Poaceae ortholog genes that are transcribed into DUF1645 domain-containing proteins in relation to the grain length,grain weight,and drought stress-tolerance of rice.The transgenic plants with overexpressing or heterologous high levels of Poaceae OsSGL ortholog genes exhibited longer grain size than the wild type plants.Larger cells were seen in panicles of the four transgenic lines with paraffin sectioning and scanning electron microscopy analyses.In addition,four Poaceae OsSGL ortholog genes positively affected the drought tolerance of rice.Four transgenic plants displayed higher resistance to drought stress at the seedling and vegetative stages.RNA-sequencing and qRT-PCR results indicated that over-or heterologous-expression of four Poaceae OsSGL ortholog genes also affected the transcriptome of rice plants.These genes may play a role in auxin and cytokinin biosynthesis and their transduction pathways.Taken together,these results suggested that the four OsSGL orthologs have a conserved function in the regulation of stress-tolerance and cell growth by modulating hormonal biosynthesis and signaling. 展开更多
关键词 graminaceous cereal crop Os SGL gene drought resistance grain length grain weight
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Control Effects of Mixture of Metamifop and Cyhalofop-butyl on Annual Weeds Barnyard Grass in Direct-seeding Paddy Field 认领 引用 被引量：1
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作者 ZHU Xiong-mei DENG Hui-fang +4 位作者 TAN Xian-sheng GUO Kai-fa JIN Chen-zhong HAN Qing ZHOU You 《Agricultural Science & Technology》 CAS 2022年第1期24-27,共4页
In order to explore the control effects of 10% metamifop EC and 20%cyhalofop-butyl OD on barnyard grass(Echinochloa crusgalli(L.) Beauv.) as well as their safety to rice in rice direct-seeding paddy field, field effic... In order to explore the control effects of 10% metamifop EC and 20%cyhalofop-butyl OD on barnyard grass(Echinochloa crusgalli(L.) Beauv.) as well as their safety to rice in rice direct-seeding paddy field, field efficacy experiments were carried out in Taoyuan County and Hanshou County of Hunan Province. The results showed that the control effect of different pesticide treatments on barnyard grass was good, and the control effect reached more than 93%. Of which, 10% metamifop EC 2 250 mL/hm2+20% cyhalofop-butyl OD 1 500 mL/hm2had the best control effect,exceeding 99% and each treatment had no significantly ponisonous to rice. However,considering cost-saving and effect-increasing, it was recommended to use 10%metamifop EC 1 050 mL/hm2mixed with 20% cyhalofop-butyl OD 1 050 mL/hm2for the control of barnyard grass, which not only had the effect of controlling barnyard grass, but also was safety to rice. 展开更多
关键词 Barnyard grass Mixture Metamifop Cyhalofop-butyl Control effect
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Synergistic Effects of Herbicide Adjuvants on Weeds Control and Safety Determination in Field Application 认领 引用 被引量：1
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作者 MENG Gui-yuan CHEN Yan-yan +3 位作者 HU Zeng-you DAI Bing ZHOU Jing JIN Chen-zhong 《Agricultural Science & Technology》 CAS 2019年第2期34-41,共8页
The effects of 9 plant adjuvants on the control of Stellaria japonica and Poa pratensis by 3 herbicidesor mixtures,including MCPA-Na+Clethodim,Atrazine and Bentazon,were studied by pot experiments.The results showed t... The effects of 9 plant adjuvants on the control of Stellaria japonica and Poa pratensis by 3 herbicidesor mixtures,including MCPA-Na+Clethodim,Atrazine and Bentazon,were studied by pot experiments.The results showed that all these 9 plant adjuvants could significantly promote the control effects of MCPA-Na+Clethodim on 2 weeds,except Jiabajin for Poa pratensis.The control effect on plant number and fresh weight of Stellaria japonica increased by 90.6%~100.0%and 47.6%~56.2%respectively 10 d after the treatment,and 25.1%~96.4%and 17.7%~90.4%for Poa pratensis respectively.Pharmacodynamics,Doctor lemon oil,Pamiere,Beirunsu and Wynote had better synergistic effects.Plant adjuvants had little synergistic effect on Atrazine and Bentazon in the early stage which was 10 d after the treatment.However,when it was 20 d after the treatment,adjuvants like Yaoxiaoling,Pamiere and Beirunsu could significantly increase the control effect of Atrazine on Stellaria japonica.Yaoxiaoling,Jiabajin and Beirunsu could promote the synergistic effect of Bentazon on Poa pratensis.Adding herbicide Yaoxiaoling could increase the herbicide control effect,fresh weight control effect,plant height and fresh weight inhibition rate of weeds in maize and flax fields.And the herbicide phytotoxicity was not obvious.The combinations of plant adjuvants with 4 herbicides including Atrazine,Nicosulfuron Mesotrione Atrazine,Nicosulfuron Atrazine,and Bentazon;had better control effects on weeds in maize fields,while MCPA-Na+Clethodim+adjuvants had better control over weeds in flax fields. 展开更多
关键词 Plant adjuvants Weeds Control Synergy
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